In Vitro Anti-Inflammatory, Anti-Oxidant, and Cytotoxic Activities of Four Curcuma Species and the Isolation of Compounds from Curcuma aromatica Rhizome

The genus Curcuma is part of the Zingiberaceae family, and many Curcuma species have been used as traditional medicine and cosmetics in Thailand. To find new cosmeceutical ingredients, the in vitro anti-inflammatory, anti-oxidant, and cytotoxic activities of four Curcuma species as well as the isolation of compounds from the most active crude extract (C. aromatica) were investigated. The crude extract of C. aromatica showed 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity with an IC50 value of 102.3 μg/mL. The cytotoxicity effect of C. aeruginosa, C. comosa, C. aromatica, and C. longa extracts assessed with the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 200 μg/mL were 12.1 ± 2.9, 14.4 ± 4.1, 28.6 ± 4.1, and 46.9 ± 8.6, respectively. C. aeruginosa and C. comosa presented apoptosis cells (57.7 ± 3.1% and 32.6 ± 2.2%, respectively) using the CytoTox-ONE™ assay. Different crude extracts or phytochemicals purified from C. aromatica were evaluated for their anti-inflammatory properties. The crude extract of C. aromatica showed the highest potential to inhibit NF-κB activity, followed by C. aeruginosa, C. comosa, and C. longa, respectively. Among the various purified phytochemicals curcumin, germacrone, curdione, zederone, and curcumenol significantly inhibited NF-κB activation in tumor necrosis factor (TNF) stimulated HaCaT keratinocytes. Of all compounds, curcumin was the most potent anti-inflammatory.


Introduction
The genus Curcuma is part of the family Zingiberaceae and over 120 species have been identified [1]. Many Curcuma species have been used as traditional medicine for the treatment of various diseases [2], or

Plant Material
The rhizome of C. aromatica (N: 20.1924

Extraction
The rhizomes of the four Curcuma species were cleaned, chopped, and air-dried at room temperature for three days. The air-dried rhizomes (1 kg) of each plant were macerated in EtOAc (3 × 10 L) at room temperature. The extracts were filtered and evaporated under reduced pressure to obtain the EtOAc extracts of C. aromatica (21.67 g), C. comosa (24.49 g), C. aeruginosa (20.21 g), and C. longa (19.76 g). Additionally, dried powder (100 g) of each plant was extracted with 80% ethanol (3 × 500 mL) at room temperature. Removal of the solvent under reduced pressure yielded the crude ethanolic extracts of C. aromatica (2.2 g), C. comosa (2.5 g), C. aeruginosa (2.0 g), and C. longa (2.1 g).

Characterization of Curcuma Extracts by UPLC-HRMS
Crude extracts of the four Curcuma species, prepared with 80% ethanol/20% water were analyzed by Ultra-Performance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS) together with 8 of the 15 purified compounds isolated from C. aromatica, in order to determine whether these compounds were present in C. longa, C. comosa and C. aeruginosa too. Liquid chromatography analysis was performed on an Acquity ® UPLC System (Waters, Milford, MA, USA). Detection was carried out on an LTQ-Orbitrap ® XL hybrid mass spectrometer equipped with an Electrospray Ionization (ESI) source (Thermo Scientific, Waltham, MA, USA) for accurate mass. Separation was achieved on an Acquity UPLC ® Peptide BEH C18 column (2.1 × 100 mm, 1.7 µm, Waters corporation ® , Wexford, Ireland) using a gradient containing water with 0.1% (v/v) formic acid (A) and acetonitrile (B). The gradient elution was performed as follows: 0-2 min eluent B 2%; 2-18 min eluent B 2-100%; 18-20 min eluent B 100%; 21-25 min column equilibration-eluent B 2%. A flow rate of 0.4 mL/min was employed for elution. The column was maintained at 40 • C, the samples at 7 • C, and the flow rate was set to 0.4 mL/min. The 80% ethanol extracts (10 µL at 300 µg/mL) were injected. All samples were analyzed in the full scan m/z range of 115-1000, in negative and positive mode at a resolving power of 30,000 and data-dependent MS/MS events were acquired. In both modes the data-dependent acquisition was simultaneously performed using a collision induced dissociation C-trap (CID) with normalized collision energy at 35 V and a mass resolution of 10,000. In negative mode capillary temperature was set to 350 • C and the source voltage was 2.7 kV. Tube lens and capillary voltage were respectively tuned at −100 V and −30 V. In positive mode capillary temperature was set to 350 • C and the source voltage was 3.50 kV. Tube lens and capillary voltage were respectively tuned at +120 V and +40 V. In both modes the arbitrary units were used for sheath gas, auxiliary gas, and sweep gas was nitrogen at (40, 10, 0 arbitrary units, respectively). The control of the system and the spectral interpretation was performed using the XcaliburTM (Version 2.2, Thermo Scientific, Waltham, MA, USA) software.

DPPH Radical-Scavenging Activity Assay
The antioxidant activity was determined by the DPPH radical scavenging assay as described previously, with slight modifications [24]. In brief, 100 µL of extracts and compounds at different concentrations were mixed with 100 µL of 60 µM DPPH methanol solution in a 96-well microplate. The solution was incubated at room temperature in darkness for 30 min, then absorbance was measured at 517 nm. Ascorbic acid was used as positive control. The DPPH radical scavenging activity was expressed as the concentration at 50% inhibition (IC 50 ), which was calculated by plotting percent inhibition against concentration of the sample.

ABTS Radical Cation Scavenging Assay
The ABTS radical cation scavenging activity of extracts and compounds was determined using the method described previously [24] with some modifications. The ABTS ·+ solution was prepared from the reaction of equal volumes of 7 mM of ABTS and 2.45 mM potassium persulfate in a dark place at room temperature for 16 h before use. Prior to the assay, the ABTS ·+ solution was adjusted to the absorbance of 0.70 ± 0.05 at 734 nm with EtOH. Twenty microliters of extracts and compounds at different concentrations were mixed with 180 µL of ABTS ·+ solution in a 96-well microplate and incubated at room temperature for 5 min. Next, the absorbance was measured at 734 nm. Ascorbic acid was used as positive control. The ABTS radical cation scavenging activity was expressed as the concentration at 50% inhibition (IC 50 ), which was calculated by plotting percent inhibition against concentration of the sample.

MTT Assay
Adverse anti-proliferative or toxic effects of various extracts and purified phytochemicals compounds on HaCaT cells were evaluated by MTT colorimetric assay. Cells were seeded into 96-well plates at 2 × 10 4 cells/well and incubated under the abovementioned conditions for 24 h. The extracts or pure compounds at different concentrations were added for another 24 h, after which 10 µL of MTT reagent (5 mg/mL) was added to each well and incubated for 4 h. Cells were lysed with 90 µL 10 mM HCl solution containing 10% SDS and OD value was measured at 595 nm with the Envision Plate Reader (Perkin Elmer, USA). Withaferin A was used as positive control.

CytoTox-ONE™ Cytotoxicity Assay
Cell cytotoxicity was measured by determining membrane integrity of HaCaT cells following treatment with crude extracts or purified phytochemicals by means of the CytoTox-ONE™ Assay according to the manufacturer's instructions (Promega, WI, USA). In brief, cells were plated at 2 × 10 4 cells/well in 96-well plates and incubated under the above-mentioned conditions for 24 h. Extracts or pure compounds at different concentrations were added to the cells and left to incubate for 24 h at 37 • C and 5% CO 2 . After incubation, the assay plates were transferred to 22 • C for 5 min, 100 µL of the CytoTox-ONE™ reagent was added to all wells and incubated at 22 • C for 10 min. After that, 50 µL of stop solution was added to all wells and plates were shaken at 500 rpm for 10 s. The fluorescence signal was measured with an excitation wavelength of 560 nm and an emission wavelength of 590 nm with the Tecan GENios Microplate Reader (Tecan Trading AG, Männedorf, Switzerland). Withaferin A was used as positive control. The triplicate wells without cells were used as negative control to determine background fluorescence. Vehicle control was triplicate cells with untreated cells, the same solvent used to deliver the test compounds. In addition, 2 µL of lysis solution was used as maximum LDH release control.

Luciferase Assay
NFκB-luciferase-dependent reporter assays were performed in HaCaT cells stably expressing p(NFκB) 3 50-luc as previously described [25]. In brief, cells were plated at a density of 10 5 cells/well in 24-well plates and grown overnight. Cells were subsequently treated with a dose range of crude extracts or purified compounds for 2 h, followed by TNF stimulation (2 ng/mL) for 6 h. Finally, cells were lysed in 1 X lysis buffer (25 mM Tris-phosphate (pH 7.8), 2 mM DTT, 2 mM CDTA, 10% glycerol, and 1% Triton X-100) and 25 µL of lysate was assayed for luciferase activity by adding 50 µL of luciferase substrate (1 mM luciferin or luciferin salt, 3 mM ATP, and 15 mM MgSO 4 in 30 mM HEPES buffer, pH 7.8). After 10 s of mixing, bioluminescence was measured for 1 s using the Envision multilabel reader (Perkin Elmer, Waltham, MA, USA). Withaferin A was used as positive control.

Data Analysis
All analyses were performed in triplicate and data were expressed as means ± standard deviation (SD) from at least three independent biological experiments. The results were analyzed by one-way analysis of variance (ANOVA) with the Dunnett test, significant difference (p < 0.05) using IBM SPSS Statistics, version 23 (IBM Crop.).
Biomolecules 2020, 10, x FOR PEER REVIEW 5 of 14 °C and 5% CO2. After incubation, the assay plates were transferred to 22 C for 5 min, 100 μL of the CytoTox-ONE™ reagent was added to all wells and incubated at 22 C for 10 min. After that, 50 μL of stop solution was added to all wells and plates were shaken at 500 rpm for 10 s. The fluorescence signal was measured with an excitation wavelength of 560 nm and an emission wavelength of 590 nm with the Tecan GENios Microplate Reader (Tecan Trading AG, Männedorf, Switzerland). Withaferin A was used as positive control. The triplicate wells without cells were used as negative control to determine background fluorescence. Vehicle control was triplicate cells with untreated cells, the same solvent used to deliver the test compounds. In addition, 2 μL of lysis solution was used as maximum LDH release control.

Luciferase Assay
NFκB-luciferase-dependent reporter assays were performed in HaCaT cells stably expressing p(NFκB)350-luc as previously described [25]. In brief, cells were plated at a density of 10 5 cells/well in 24-well plates and grown overnight. Cells were subsequently treated with a dose range of crude extracts or purified compounds for 2 h, followed by TNF stimulation (2 ng/mL) for 6 h. Finally, cells were lysed in 1 X lysis buffer (25 mM Tris-phosphate (pH 7.8), 2 mM DTT, 2 mM CDTA, 10% glycerol, and 1% Triton X-100) and 25 µ L of lysate was assayed for luciferase activity by adding 50 µ L of luciferase substrate (1 mM luciferin or luciferin salt, 3 mM ATP, and 15 mM MgSO4 in 30 mM HEPES buffer, pH 7.8). After 10 s of mixing, bioluminescence was measured for 1 s using the Envision multilabel reader (Perkin Elmer, Waltham, MA, USA). Withaferin A was used as positive control.

Data Analysis
All analyses were performed in triplicate and data were expressed as means  standard deviation (SD) from at least three independent biological experiments. The results were analyzed by one-way analysis of variance (ANOVA) with the Dunnett test, significant difference (p < 0.05) using IBM SPSS Statistics, version 23 (IBM Crop.).

Characterization of Curcuma Extracts by UPLC-HRMS
Eight of the purified compounds, germacrone (1), curdione (2), dehydrocurdione (3), zederone (5) curcumenol (6), zedoarondiol (12), curcumin (14), and β-sitosterol (15), were analyzed by UPLC-HRMS, together with the 80% EtOH extracts of C. aromatica, C. longa, C. comosa, and C aeruginosa ( Figure S1, Supplementary Material). Except for compounds 12 and 15, all compounds were detected in ESI + mode, while 5 and 13 could be detected in ESI + and ESI − mode. Table 1 shows the retention time and MS data obtained for the purified compounds. In addition, it is indicated whether these compounds could be detected in the crude extracts. Compounds 12 and 15 were not clearly detected in either of the detection modes, possibly due to poor ionization properties or their low abundance.
As expected, all six detected compounds were found in the crude extract of C. aromatica, since the compounds were purified from this Curcuma species as described in Sections 2.1 and 3.1 Also C. longa was found to contain these six compounds. Five out of six compounds could be identified in the 80% EtOH extracts of C. aeruginosa; only curcumin (13) was found to be absent in this Curcuma species. The C. comosa extract did not contain curcumin either, nor did it contain curcumenol. Our results about the phytochemical composition of different Curcuma species are in line with results reported by other groups [26,27].
Since curcumin was only detected in C. aromatica and C. longa and not in C. comosa and C. aeruginosa, the activity of the first two extracts may in part be attributed to the presence of curcumin. However, since C. comosa showed antioxidant activity similar to C. longa, and C. aeruginosa showed significant antioxidant activity too, curcumin cannot be the only active compound and other constituents might also contribute too to overall antioxidant activity. Values are the mean ± SD, n = 3; DPPH: 2,2-diphenyl-1-picrylhydrazyl; ABTS: 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt. the 80% EtOH extracts of C. aeruginosa; only curcumin (13) was found to be absent in this Curcuma species. The C. comosa extract did not contain curcumin either, nor did it contain curcumenol. Our results about the phytochemical composition of different Curcuma species are in line with results reported by other groups [26,27].

Antioxidant Activity
The antioxidant radical scavenging activity of extracts were evaluated using DPPH and ABTS assays (Table 2), and purified compounds were tested in the DPPH assay as shown in Figure 2. Regarding antioxidant activity, the C. aromatica extract showed the most promising IC50 values (102.4  1.9, 127.0  1.9 μg/mL), followed by C. longa (134.9  1.5, 170.8  1.6 μg/mL), C. comosa (137.7  5.2, 171.9  1.9 μg/mL), and C. aeruginosa (187.4  22.1, 217.9  1.8 μg/mL). Ascorbic acid was used as positive control, with IC50 values of 1.80  0.01 and 5.2  0.8 for DPPH and ABTS assay, respectively. In addition, curcumin exhibited strong antioxidant activity with 68.9%  0.6% percent inhibition of at 25 μg/mL, whereas other compounds showed moderate activities, see Figure 2. Since curcumin was only detected in C. aromatica and C. longa and not in C. comosa and C. aeruginosa, the activity of the first two extracts may in part be attributed to the presence of curcumin. However, since C. comosa showed antioxidant activity similar to C. longa, and C. aeruginosa showed significant antioxidant activity too, curcumin cannot be the only active compound and other constituents might also contribute too to overall antioxidant activity.

Cell Viability and Cytotoxicity
Cell viability and cytotoxicity of crude extracts and pure compounds were assessed by MTT assay and the CytoTox-ONE™ Homogeneous Membrane Integrity Assay using HaCaT keratinocyte cells, respectively. The MTT colorimetric assay estimates the number of viable cells based on the ability of mitochondrial enzymes to reduce the tetrazolium dye MTT to a purple colored formazan [37], whereas the CytoTox-ONE™ assay is a fluorometric-based method to detect loss of membrane integrity of dying cells. MTT results showed that exposure to 200 µg/mL of C. aeruginosa, C. comosa, C. aromatica, or C. longa extract inhibited the growth of cells, with relative percentages of cell viability being 12.1 ± 2.9, 14.4 ± 4.1, 28.6 ± 4.1, and 46.9 ± 8.6, respectively (Figure 3a). Interestingly, CytoTox-ONE™ showed a slightly different outcome with estimated cell death being lower compared to the MTT results. Treatment of HaCaT cells with 200 µg/mL concentrations of C. aeruginosa and C. comosa extract resulted in 57.7 ± 3.1% and 32.6 ± 2.2% cell death respectively, while no cytotoxicity could be observed with C. aromatica and C. longa treatments at the same concentration (Figure 4a). This suggests that all extracts mainly affect mitochondrial reduction capacity and cell proliferation, and that only C. aeruginosa and C. comosa extracts negatively impact membrane integrity at concentrations above 100 µg/mL [38][39][40]. In contrast, none of the purified phytochemicals inhibit cell viability (MTT) or cytotoxicity (CytoTox-One™) at concentrations 1-20 µM, whereas a reference cytotoxic anti-cancer compound withaferin A [28] dose dependently kills the HaCaT cells, as shown in Figures 3b and 4b. and the CytoTox-ONE™ Homogeneous Membrane Integrity Assay using HaCaT keratinocyte cells, respectively. The MTT colorimetric assay estimates the number of viable cells based on the ability of mitochondrial enzymes to reduce the tetrazolium dye MTT to a purple colored formazan [37], whereas the CytoTox-ONE™ assay is a fluorometric-based method to detect loss of membrane integrity of dying cells. MTT results showed that exposure to 200 μg/mL of C. aeruginosa, C. comosa, C. aromatica, or C. longa extract inhibited the growth of cells, with relative percentages of cell viability being 12.1  2.9, 14.4  4.1, 28.6  4.1, and 46.9  8.6, respectively (Figure 3a). Interestingly, CytoTox-ONE™ showed a slightly different outcome with estimated cell death being lower compared to the MTT results. Treatment of HaCaT cells with 200 µg/mL concentrations of C. aeruginosa and C. comosa extract resulted in 57.7  3.1% and 32.6  2.2% cell death respectively, while no cytotoxicity could be observed with C. aromatica and C. longa treatments at the same concentration (Figure 4a). This suggests that all extracts mainly affect mitochondrial reduction capacity and cell proliferation, and that only C. aeruginosa and C. comosa extracts negatively impact membrane integrity at concentrations above 100 μg/mL [38][39][40]. In contrast, none of the purified phytochemicals inhibit cell viability (MTT) or cytotoxicity (CytoTox-One™) at concentrations 1-20 μM, whereas a reference cytotoxic anti-cancer compound withaferin A [28] dose dependently kills the HaCaT cells, as shown in Figures 3b and 4b. (a) (b)

Anti-Inflammatory Activity
HaCaT NF-κB reporter gene cells were left untreated or pretreated for 2 h with various crude extracts or its purified phytochemicals, followed by 3 h combination treatment with the proinflammatory stimulus TNF. After 5 h treatment, cells were lysed and corresponding luciferase reporter gene activity was measured in lysates in presence of ATP/luciferin reagent (Promega, WI, USA) by measuring the total emitted bioluminescence (relative light units, RLU) during 30s (Envision multiplate reader, Perkin Elmer). As expected, and as shown in Figure 5a, the proinflammatory NF-κB activator TNF strongly increases luciferase gene expression in HaCaT NF-κB reporter cells, as compared to the control samples without TNF. Upon combination treatment of the different extracts with TNF, we observed dose dependent decrease of luciferase gene expression for all four extracts, suggesting anti-inflammatory effects on NF-κB activity. C. aromatica showed the strongest antiinflammatory NF-κB effects, followed by C. aeruginosa, C. comosa, and C. longa, respectively.
Next, stable phytochemicals isolated in sufficient quantities isolated from C. aromatica were further evaluated for their NF-κB inhibiting activity in TNF stimulated HaCaT keratinocytes, as compared to the reference inhibitor compound withaferin A [41]. As shown in Figure 5b, curcumin was found to be the most potent NF-κB inhibitor, although less potent the reference NF-κB inhibitor withaferin A, in line with previous research [11,41]. C. aromatica, which contains curcumin, indeed was the most potent NF-kB inhibiting extract. Thus, it's traditional use in the prevention and treatment of inflammatory diseases may be justified. However, the other three extracts, of which C. longa contains curcumin, whereas C. comosa and C. aeruginosa do not, show a comparable activity. This suggests that besides curcumin, additional constituents may be responsible for NF-κB inhibition in C. comosa and C. aeruginosa extracts. Indeed, germacrone, curdione, zederone, and curcumenol

Anti-Inflammatory Activity
HaCaT NF-κB reporter gene cells were left untreated or pretreated for 2 h with various crude extracts or its purified phytochemicals, followed by 3 h combination treatment with the pro-inflammatory stimulus TNF. After 5 h treatment, cells were lysed and corresponding luciferase reporter gene activity was measured in lysates in presence of ATP/luciferin reagent (Promega, WI, USA) by measuring the total emitted bioluminescence (relative light units, RLU) during 30s (Envision multiplate reader, Perkin Elmer). As expected, and as shown in Figure 5a, the proinflammatory NF-κB activator TNF strongly increases luciferase gene expression in HaCaT NF-κB reporter cells, as compared to the control samples without TNF. Upon combination treatment of the different extracts with TNF, we observed dose dependent decrease of luciferase gene expression for all four extracts, suggesting anti-inflammatory effects on NF-κB activity. C. aromatica showed the strongest anti-inflammatory NF-κB effects, followed by C. aeruginosa, C. comosa, and C. longa, respectively.
Biomolecules 2020, 10, x FOR PEER REVIEW 11 of 14 show moderate inhibition of NF-κB reporter gene expression in TNF stimulated HaCaT keratinocytes too. In addition, zedoarondiol and β-sitosterol show strong NF-κB inhibition, although they may be low abundant, since UPLC-HRMS analysis failed to detect significant amounts in the four extracts.

Conclusions
Sesquiterpenes are major bioactive constituents in the rhizome extract of C. aromatica. Of the four Curcuma species, C. aromatica, with its secondary metabolite curcumin, showed the highest Next, stable phytochemicals isolated in sufficient quantities isolated from C. aromatica were further evaluated for their NF-κB inhibiting activity in TNF stimulated HaCaT keratinocytes, as compared to the reference inhibitor compound withaferin A [41]. As shown in Figure 5b, curcumin was found to be the most potent NF-κB inhibitor, although less potent the reference NF-κB inhibitor withaferin A, in line with previous research [11,41]. C. aromatica, which contains curcumin, indeed was the most potent NF-kB inhibiting extract. Thus, it's traditional use in the prevention and treatment of inflammatory diseases may be justified. However, the other three extracts, of which C. longa contains curcumin, whereas C. comosa and C. aeruginosa do not, show a comparable activity. This suggests that besides curcumin, additional constituents may be responsible for NF-κB inhibition in C. comosa and C. aeruginosa extracts. Indeed, germacrone, curdione, zederone, and curcumenol show moderate inhibition of NF-κB reporter gene expression in TNF stimulated HaCaT keratinocytes too. In addition, zedoarondiol and β-sitosterol show strong NF-κB inhibition, although they may be low abundant, since UPLC-HRMS analysis failed to detect significant amounts in the four extracts.

Conclusions
Sesquiterpenes are major bioactive constituents in the rhizome extract of C. aromatica. Of the four Curcuma species, C. aromatica, with its secondary metabolite curcumin, showed the highest antioxidant activity and most potent anti-inflammatory properties with the lowest toxicity. Besides curcumin, we purified additional anti-inflammatory bioactives in C. aromatica, C. aeruginosa, C. comosa, and C. longa, such as germacrone, curdione, zederone, curcumenol, zedoarondiol, and β-sitosterol present, which deserve further investigation.
In conclusion, our results suggest that the rhizome of C. aromatica holds promise to be developed as a safe cosmeceutical or functional skin care products for anti-aging and to reduce inflammatory skin irritation.