Next Article in Journal
High-Density Lipoprotein Cholesterol in Age-Related Ocular Diseases
Next Article in Special Issue
Vitamin K in Vertebrates’ Reproduction: Further Puzzling Pieces of Evidence from Teleost Fish Species
Previous Article in Journal
Phenolic Compounds Exerting Lipid-Regulatory, Anti-Inflammatory and Epigenetic Effects as Complementary Treatments in Cardiovascular Diseases
Previous Article in Special Issue
New Opportunities for Endometrial Health by Modifying Uterine Microbial Composition: Present or Future?
Open AccessReview

Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems

1
Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic
2
Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, São Paulo State University, Botucatu, SP 18618-970, Brazil
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(4), 644; https://doi.org/10.3390/biom10040644
Received: 9 March 2020 / Revised: 13 April 2020 / Accepted: 14 April 2020 / Published: 22 April 2020
(This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization)
Spermatogenesis is a continuous and dynamic developmental process, in which a single diploid spermatogonial stem cell (SSC) proliferates and differentiates to form a mature spermatozoon. Herein, we summarize the accumulated knowledge of SSCs and their distribution in the testes of teleosts. We also reviewed the primary endocrine and paracrine influence on spermatogonium self-renewal vs. differentiation in fish. To provide insight into techniques and research related to SSCs, we review available protocols and advances in enriching undifferentiated spermatogonia based on their unique physiochemical and biochemical properties, such as size, density, and differential expression of specific surface markers. We summarize in vitro germ cell culture conditions developed to maintain proliferation and survival of spermatogonia in selected fish species. In traditional culture systems, sera and feeder cells were considered to be essential for SSC self-renewal, in contrast to recently developed systems with well-defined media and growth factors to induce either SSC self-renewal or differentiation in long-term cultures. The establishment of a germ cell culture contributes to efficient SSC propagation in rare, endangered, or commercially cultured fish species for use in biotechnological manipulation, such as cryopreservation and transplantation. Finally, we discuss organ culture and three-dimensional models for in vitro investigation of fish spermatogenesis. View Full-Text
Keywords: spermatogonial stem cell (SSC); fish; spermatogenesis; florescence-activated cell sorting (FACS); magnetic-activated cell sorting (MACS); germ cell culture spermatogonial stem cell (SSC); fish; spermatogenesis; florescence-activated cell sorting (FACS); magnetic-activated cell sorting (MACS); germ cell culture
Show Figures

Figure 1

MDPI and ACS Style

Xie, X.; Nóbrega, R.; Pšenička, M. Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems. Biomolecules 2020, 10, 644.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop