Critical Impact of Different Conserved Endoplasmic Retention Motifs and Dopamine Receptor Interacting Proteins (DRIPs) on Intracellular Localization and Trafficking of the D2 Dopamine Receptor (D2-R) Isoforms

The type 2 dopamine receptor D2 (D2-R), member of the G protein-coupled receptor (GPCR) superfamily, exists in two isoforms, short (D2S-R) and long (D2L-R). They differ by an additional 29 amino acids (AA) in the third cytoplasmic loop (ICL3) of the D2L-R. These isoforms differ in their intracellular localization and trafficking functionality, as D2L-R possesses a larger intracellular pool, mostly in the endoplasmic reticulum (ER). This review focuses on the evolutionarily conserved motifs in the ICL3 of the D2-R and proteins interacting with the ICL3 of both isoforms, specifically with the 29 AA insert. These motifs might be involved in D2-R exit from the ER and have an impact on cell-surface and intracellular localization and, therefore, also play a role in the function of dopamine receptor signaling, ligand binding and possible homo/heterodimerization. Our recent bioinformatic data on potential new interaction partners for the ICL3 of D2-Rs are also presented. Both are highly relevant, and have clinical impacts on the pathophysiology of several diseases such as Parkinson’s disease, schizophrenia, Tourette’s syndrome, Huntington’s disease, manic depression, and others, as they are connected to a variety of essential motifs and differences in communication with interaction partners.


Dopamine Receptors
G-protein-coupled receptors (GPCRs), also termed seven-transmembrane receptors (7TMRs) are by far the largest family of membrane-bound receptors, which are involved in the regulation of the neurotransmitter dopamine effects as one of their targets [1]. Based on functional, structural, and pharmacological properties, five types of dopamine receptors have been described, that belong to

Dopamine Receptor Type 2 (D 2 -R)
The D 2 -R is a key component of the dopamine system that is present in two alternatively spliced transcripts of the Drd2 gene and classified as short (D 2S -R) and long (D 2L -R) receptor isoforms. The long isoform differs from the short one only by the presence of an additional 29 amino acids (AA) encoded by exon 5 in the ICL3 of the D 2L -R [17][18][19]. The inclusion is interspersed between the AA lysine (K241) and glutamic acid (E271). D 2S -R in mice and rats are made up of 415 AA and D 2L -R is made up of 444 AAs. Human D 2S -R and D 2L -R are shorter than murine and rat equivalents by one AA, consisting of 414 and 443 AAs, respectively. The isoleucine is missing between lysine (K331) and aspartic acid (D332). This region might have an essential role in the functional differences between both D 2 -R isoforms such as interactions related to G-proteins [20][21][22], post-translation modification and cell localization [11,23]. D 2 -R isoforms also indicate different in vivo functions, whereby D 2L -R primarily acts at postsynaptic and D 2S -R in presynaptic dopaminergic transmissions [24,25]. Data acquired on genetically engineered D 2 -R mouse model indicates additional evidence for different roles of two isoforms in cognitive and motor functions [24], responsiveness to cocaine exposure [26], and therapeutic effects of antipsychotic drugs [27]. Furthermore, they are expressed in the same cell types with more abundant expression of the D 2L -R isoform over D 2S -R, but with differences in their intracellular localization. While D 2S -R is primarily localized on the plasma membrane (PM), a substantial fraction of D 2L -R is located intracellularly, especially in the perinuclear compartments around the Golgi apparatus (GA) [14] and endoplasmic reticulum (ER) [23].
The D 2 -R is the most commonly studied dopamine receptor subtype since the majority of antipsychotic drugs act as D 2 -R antagonists in the mesolimbic dopaminergic system [28]. As a primary target for atypical and typical antipsychotic drugs and treatment of the Parkinson's disease, many of those agents can cause potentially life-threatening and severe side effects due to the promiscuous activities against related D 2 -Rs [29]. Precisely because of this reason, it is necessary to be familiar with the details of the dopamine receptor's complex structure and functions.

Localization Differences Between D 2S -R and D 2L -R
D 2 -R isoforms localization is neither species nor tissue specific. They were found in different tissues, but in highly variable ratios [25,30,31]. D 2S -R is predominantly localized in the PM [14,22,32], whereas an intracellular D 2L -R reservoir has been reported in the primates brain [33] and several cell lines [14,22,23].
The primary intracellular localization of the D 2L -R in transiently transfected HEK-293, COS-7, and HeLa is the ER [23], whereas in transfected NG108-15 cells intracellularly localized D 2L -R is predominantly co-localized with the GA matrix protein marker GM130 [14]. At the level of confocal microscopy, higher proportions of D 2L -R than D 2S -R were retained intracellularly in heterologous cell lines [22,23]. This finding could be due to the retention of overexpressed or incorrectly folded tagged receptors in the ER. However, immunoelectron microscopy also revealed the predominant intracellular localization of the D 2L -R [34] in monkey dopaminergic neurons, and that these sites are cisterns of GA and ER. Ligand-promoted recruitment of the D 2L -R [35], D 4 -R [36] and other GPCRs, such as thrombin receptors (PAR1 and PAR2), D 1 -Rs, and opioid receptors on the PM presented additional evidence for the existence of functional, pre-existing intracellular stores (reviewed by Achour, 2008) [37]. N-terminal glycosylation of different GPCR's has a vital role in cell surface receptor expression. Mutations of potential N-terminal glycosylation sites found for D 2 -R (N5, N17, and N23) lead to decreased surface expression for D 2 -R, showing their important role in receptor distribution [38]. Fishburn et al. performed studies regarding post-translational processing of the D 2L -R and D 2S -R isoforms [39]. Three post-translational states were observed in both receptor isoforms: a newly synthesized protein (35 kDa), a partially glycosylated product (45 kDa) and a fully glycosylated receptor (70 kDa) [39].
A difference in the processing of the mature receptor was observed. The initial N-glycosylation of the newly synthesized receptor protein occurred shortly after synthesis in both D 2 -R isoforms, suggesting that a rapid and efficient maturation towards partially glycosylated product occurs in D 2 -R. However, a marked difference was observed in subsequent N-linked glycosylation, where D 2L -R showed slower production of fully glycosylated proteins in comparison to the D 2S -R. Additionally, they showed that 20% of the D 2L -R remains in the partially processed form and never undergoes the second stage of N-linked glycosylation.

Functional Differences between D 2S -R and D 2L -R
Identifying probable functional differences between D 2S -R and D 2L -R has been the subject of numerous studies. Depending on the site of action and the effect on D 2 -R-mediated responses, isoforms have different and likely antagonistic functions in vivo [24]. D 2S -R is mainly a presynaptic receptor, but at the postsynaptic level, it negatively modulates D 1 -R-dependent responses. In contrast, D 2L -R is found predominantly at postsynaptic sites where it acts synergistically with D 1 -Rs [24].
The location of the inclusion in ICL3 also led to the assumption that it may affect the specificity of the interaction with G-proteins and the sequential activation of specific effector proteins.
Several studies have shown that structural differences between isoforms can determine the specificity of interactions with G-proteins [10,40]. A recent study showed the preferential coupling of both D 2 -R isoforms with G-proteins (G i1 and G i2 ), due to the differences in ICL3, which affect receptor behavior [11,41]. Results obtained with the messenger gene construct controlled by the cAMP response promoter suggest constitutive, i.e., agonist-independent D 2L -R activity [42]. Studies with D 2 -R knockout mice provided additional evidence for their diverse roles in motor and cognitive functions [43], sensitivity to cocaine [44], and therapeutic/side effects of antipsychotic agents [45].

The ER Retention Motifs in GPCRs and Both D 2 -R Isoforms
The synthesis and transfer of newly formed proteins via secretory pathways from the ER to the PM is a complex process involving different mechanisms and many additional proteins and motifs that are important in protein interaction and enable their proper formation, quality control, selective retention, and transport [46]. Mechanisms that regulate the secretory transport of GPCRs or their transfer to the cell surface are poorly elucidated [47,48]. It is known that the interconnection of the same or different GPCRs, homo-and heterodimerization, is essential in the transport of GPCRs in families A and C, but not for representatives of family B on the PM [49]. Transfer of the protein to the PM requires control of transport from the ER via the GA to the PM. This process is regulated by COPI and COPII vesicles [50]. COPII-coated transfer vesicles serve anterograde transport from the ER to GA, whilst transport between GA cisternae and retrograde transport from GA to ER takes place with COPI-coated vesicles. Improperly synthesized proteins or those having exposed sequences encoding motives for retention in the ER are transported retrogradely into the ER by COPI-coated vesicles [51]. Moreover, correctly folded proteins might be retained in the ER because they hold ER retention motifs, which prevent their export from the ER. Three types of ER retention motifs have been identified in the intracellular domains of various proteins: KDEL, KKXX, and RXR type motifs [46]. The presence of specific conserved sequences, so-called ER retention signals, could be responsible for preventing D 2 -R proteins from leaving the ER. Since the level of GPCR expression dictates the magnitude of cellular responses elicited by a signal at the PM, which is the balance of elaborately regulated endocytic and exocytic trafficking, it is crucial to know the motifs as well as the proteins involved in this interplay.

KDEL and KKXX Motifs
The KDEL motif is a short C-terminal retrieval signal (Lys-Asp-Glu-Leu) identified in ER luminal chaperone proteins, such as immunoglobulin heavy chain-binding protein (BiP) and other soluble ER resident proteins [20,52,53]. The KDEL receptor recognizes this motif in the post-ER compartments, which mediates retrograde transport to the ER by COPI coatomer structures [54]. For the proper sorting of cargo into COPI vesicles, a Ras-like small GTPase ADP-ribosylation factor 1 (ARF1) activation is required [55]. ARFGAP1 activates ARF1 by hydrolysis of GTP to GDP [56]. Ligand binding on the luminal side of the KDEL receptor induces interaction with ARFGTP1 on the cytoplasmic side of the receptor, resulting in the recruitment of ARFGTP1 from the cytosol to the PM leading to ARF1 activation [57].
In zebrafish D 2 -Rs and D 3 -Rs, the expression of gene Hsp47 was identified, which is an ER-resident collagen-specific chaperone with a C-terminal KDEL retention motif and plays a fundamental role in the folding, stability, and intracellular transport of procollagen triple helices [58].
The KDEL receptor cycles between the ER and the GA and its affinity for KDEL containing proteins changes between these two compartments. Retrieval of proteins mediated by the KDEL receptor can occur from different sites, ranging from early Golgi complex locations to trans Golgi networks [30]. In the GA, the KDEL receptor could associate with Gα o , one of the abundant Gα subunit and regulate receptor trafficking through G-proteins [59].
In addition to KDEL, the di-lysine motif (KKXX), have been identified as retrieval signal, important for recycling proteins from the GA back to the ER [60]. These signals are necessary for determining the localization of modified secretory and PM proteins in the ER [30].
Type I integral membrane proteins, ERGIC53, and p24 family proteins contain di-lysine KKXX motifs [46]. This carboxyl-terminal retrieval signal usually consists of two lysine residues on positions -3 and -4 relative to the C-terminus, followed by any amino acid [61]. The KKXX signal is evolutionarily conserved as it also appears in yeast [20]. Like KDEL, the KKXX motif also serves as a retrieval signal for the transport of proteins from GA by COPI vesicles, although it binds directly with coatomer structures and does not require a receptor [62].

RXR and RSRR Motifs
The RXR motif, and in some proteins, RSRR, has been found on different proteins where it disables the exit of proteins from the ER. Initially, they were found in ion channels and also in several GPCRs [46,63]. The first discovered of two arginine retention signals (the RXR and RSRR) are known to be located at the C-terminal end of the Kir 6.2 potassium channel (RXR signal) and a GPCR representative, the gamma-aminobutyric acid type B1 receptor (GABA B1 ; RSRR signal) [27]. The GABA B1 receptor contains the C-terminal RXR type ER retention motif RSRR, which prevents protein release from the ER. GABA B1 is functionally impaired in terms of ligand binding when expressed alone, whereas GABA B2 is nonfunctional in its signaling properties. Only when co-expressed with GABA B2 , GABA B1 receptor releases from the ER and translocates to the cell surface [27,64]. Upon co-expression, the RSRR retention signal of GABA B1 is proposed to be masked by interaction of the C-terminus of both subunits due to highly stable α coil-coil interactions. Therefore, GABA B1 and GABA B are functionally combined of distinct subunits as obligatory constitutive heterodimers [27,[64][65][66].
There are more examples of the RXR-type retention motif in GPCR receptors. A published study showed that in the C-terminal end of the type 2c α-adrenergic receptor (α2C-AR), there is a set of five arginine residues (RRRRR), which represent a possible retention signal of the ER type RXR [67]. The RXR-type retention motif has also been described in the ICL3 of the kainate receptor, which is a ligand-dependent ion channel [68]. Disease-causing vasopressin type 2 receptors (V2R) mutations are retained in different compartments of the early secretory pathway [69]. V2R mutants connected to nephrogenic diabetes insipidus in the contrast with the wild-type V2R are less expressed on the cell surface. Additionally, the D 2 -R RXR motifs have been revealed. In our study [70], we showed that the evolutionarily conserved arginine cluster in the insert of ICL3 of D 2L -R (R267-R269) acts as an ER retention signal and is potentially crucial for anterograde trafficking of the D 2 -R and receptor PM availability. However, we must take into account that ER exit is a highly regulated process and that one motif is not responsible solely for it. Other proposed mechanisms involving interaction with the ER-resident gatekeeper prenylated Rab acceptor 1 domain family member 3 (PRAF3) or other D 2L -R binding and interaction proteins, such as fatty acid-binding protein 3 (FABP3), which possibly bind to 29 AA in ICL3 in D 2L -R may be included (Figure 1) [71].
Additionally, the mutational analysis revealed that heteromers between the dopamine D 2 -R, adenosine A 2A and cannabinoid CB 1 receptors are stabilized by electrostatic interactions between arginine-rich motifs in the ICL3 of D 2 -R and A 2A receptors and phosphorylated casein kinase 1/2 sites in ICL3 and C-tail of the CB1 receptor, and the C-terminus of the A 2A receptor [72]. The RXR-rich motif in ICL3 of the D 2 -R is also involved in stabilizing electrostatic interactions with a di-glutamate motif in the C-terminus of the serotonin 5-HT 2A and D 1 -R [73,74]. Rabex-5-Rabaptin-5 interacting protein; TRAPPC9-Trafficking protein particle complex subunit 9.

ER Export Motifs in GPCRs and D2-R
GPCRs originate in the ER, where they are synthesized, folded, and assembled. Properly folded receptors are recruited and packaged into ER-derived COPII-coated vesicles. Transport vesicles carrying cargo receptors then migrate from the ER to the ER-Golgi intermediate complex (ERGIC), the GA, and the trans Golgi network (TGN). During their transport, receptors undergo posttranslational modifications (e.g., glycosylation). Mature receptors then move from the TGN to their destination at the PM [77]. For this process, conserved sequences and motifs essential for the exit of Likhite, N. et al. [75] used bioinformatics analysis to identify 583 RGG and RXR-type motifs in GPCRs. Approximately 34% of those were conserved in human GPCRs within the ICL3 and could serve as arginine methylation motifs. They showed that R217 and R219 within the ICL3 N-terminal end common to both D 2 -R isoforms serve as an arginine N-methyltransferase 5 (PRMT5) methylation motif important for modulating receptor signaling [75]. This corroborates with R217 and R219 location of within the Gα interaction domain (reviewed in [75]).
Furthermore, Table 1 summarizes selected motifs from the eukaryotic linear motif (ELM) [76] resources, which are potentially involved in ER-GA trafficking of the D 2 -R. The experimentally validated short linear motifs (SLiM) were manually curated after globular domain filtering, structural filtering, and context filtering.

ER Export Motifs in GPCRs and D 2 -R
GPCRs originate in the ER, where they are synthesized, folded, and assembled. Properly folded receptors are recruited and packaged into ER-derived COPII-coated vesicles. Transport vesicles carrying cargo receptors then migrate from the ER to the ER-Golgi intermediate complex (ERGIC), the GA, and the trans Golgi network (TGN). During their transport, receptors undergo post-translational modifications (e.g., glycosylation). Mature receptors then move from the TGN to their destination at the PM [77]. For this process, conserved sequences and motifs essential for the exit of GPCRs from the ER are critical. Export from the ER is the first step in the intracellular trafficking of GPCRs These motifs are found on the C-or N-terminal tail of the receptors and are therefore common to both D 2 -R isoforms. A triple phenylalanine motif [F(X) 3 F(X) 3 F] [14] has been identified in the membrane-proximal C-terminus of the D 1 -R that is required for receptor cell-surface expression [78]. However, no motifs have yet been identified on the D 2 -R. A newly identified ER-membrane-associated protein, DRiP78, binds to this motif, as described later.

D 2 -R Interaction Proteins (DRIPs)
More than 20 dopamine receptor-interacting membrane-associated or cytoplasmic D 2 -R interaction proteins (DRIPs) are known and several of them bind the ICL3 of the D 2 -R [79]. Using the informational spectrum method (ISM), a virtual spectroscopy method for investigating protein-protein interactions, the analysis of known interaction partners of IC3 of D 2 -R [79] was performed as previously described [80,81] and obtained the results presented in Table 2. ISM analysis of the IC3 D 2 -R interaction with protein partners corroborates with published data (reviewed in [79]) (Table 2, Figure 1). However, in addition to previously identified protein partners it has also been suggested that there are some new potential interaction partners.
Among previously described interaction partners, the highest affinity for the interaction with the D 2 -R was ascribed to N-methyl-D-aspartate (NMDA) receptor NR2B subunits. It was shown that a distinct region within the first 32 AA of the D 2 -R ICL3 interacts with the NR2B and disrupts the association of Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) with NR2B, reduces NR2B phosphorylation at a CaMKII-sensitive site (Ser1303), and inhibits NMDA receptor-mediated currents in medium-sized striatal neurons. The D 2 -R-NR2B interaction is therefore critical for modulating NMDA receptor-mediated currents and behavioral responsiveness to cocaine [82]. The second highest propensity for interaction with the D 2 -R was observed for prostate apoptosis response-4 (Par-4). Par-4 is a protein expressed in the nervous system, where it is known to be a regulatory component in dopaminergic signaling. It is a mediator of neuronal degeneration, and is associated with the pathogenesis of Alzheimer's disease [83]. Par-4 directly interacts with the D 2 -R via the calmodulin-binding motif in the ICL3. Furthermore, Par-4 constitutes a molecular link between impaired dopaminergic signaling and depression [84]. The N-terminal segment of the D 2 -Rs and D 3 -R was also shown to interact with neuronally enriched 4.1N protein; an interaction that contributes to the localization and stability of D 2 -Rs at the neuronal PM [85]. Similarly, filamin-A (FLN-A) also interacts with the N-terminal segment of the ICL3 of the D 2 -R and D 3 -R, and connects D-Rs with some other GPCRs, such as rhodopsin and, metabotropic glutamate receptors to the cytoskeleton, and therefore participate in their final subcellular localization [86]. The dopamine transporter (DAT) is a membrane-spanning protein that facilitate the reuptake of extracellular dopamine to the cytosol and is therefore, an essential target for cocaine, amphetamine, and some other drugs of abuse. One study showed a direct interaction between the DAT and the ICL3 (I340-Q373) of both D 2 -R isoforms. However, D 2L -R is more capable of physically interacting with the DAT [87].
The Ca 2+ -binding protein calmodulin (CaM) binds to the N-terminal portion of the ICL3 of the D 2L -R, within an Arg-rich epitope (VLRRRRKRVN) that is also involved in the binding to G i/o proteins and the adenosine A 2A receptor, with the formation of A 2A -D 2 -R heteromers [88,89]. N-ethylmaleimide-sensitive factor (NSF) is an ATPase and an essential part of the protein network responsible for different membrane fusion events, including transport through the GA and exocytosis [90]. Using immunoprecipitation and in vitro binding assays, it has been shown that NSF binds to the ICL3 of D-R (F341-Q373) and has a putative role in the interaction of D 2 -R and the Glu2 AMPA receptor [91]. Agonist stimulation of D 2 -R promotes the formation of direct protein-protein interactions between the ICL3 of the D 2 -R and the ATPase N-ethylmaleimide-sensitive factor (NSF). Spinophilin is F-actin and protein phosphatase-1-binding protein with a single PDZ domain that was identified as a protein associated with the ICL3 region of the D 2 -R. It is hypothesized to be necessary for establishing signaling complexes for dopaminergic neurotransmission through D 2 -Rs by linking receptors to downstream signaling molecules and the actin cytoskeleton [92].
Three additional hypothetical ICL3 D 2 -R interaction partners were suggested by ISM: prolactin regulatory element-binding protein (PREB), chromaffin granule amine transporter (CGAT) and trafficking protein particle complex subunit 9 (TRAPPC9). Among prospective partners, CGAT displayed the highest affinity for interacting with the ICL3 D 2 -R, followed by TRAPPC9 and PREB. For all three prospective interaction partners we were unable to find experimental evidence for the direct interaction with the ICL3 of the D 2 -R but only some indirect indication for their involvement in dopamine synthesis, transport, or D 2 -R binding. PREB is an ubiquitously expressed protein and, a member of the WD-repeat protein family, that acts as a transcriptional regulator and suppresses the expression of the adiponectin gene [93], regulates prolactin (PRL) gene expression [94] and functions as a transcriptional regulator of PRL promoter activity, and therefore might be involved in thyrotropin-releasing hormone (TRH)-induced PRL gene transcription [95]. PRL gene expression and secretion are regulated by various hormones and growth factors, including dopamine, epidermal growth factor, and thyrotropin-releasing hormone (TRH) [95]. PREB is highly expressed in the anterior pituitary. Prolactinomas are the most common pituitary tumors and are treated with the selective dopamine D 2 -R agonist cabergoline [96]. Mutation of the PREB-binding site within the promoter abrogated the ability of cabergoline to inhibit PRL promoter activity. The chromaffin granule amine transporter (CGAT), also named the vesicular monoamine transporter 1 (VMAT1), is involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells. It has a positive impact on dopamine synthesis, secretion, and transport to storage vesicles, which releases neurotransmitters into synapses as chemical messages to postsynaptic neurons [97]. The pharmaceutical industry also targets VMATs for treating hypertension, drug addiction, psychiatric disorders, Parkinson's disease, and other neurological disorders. The trafficking protein particle complex subunit 9 (TRAPPC9), also known as NIBP, belongs to the TRAPPII multiprotein complex. TRAPPC9 is involved in vesicular trafficking from the ER to the GA and promotes the activation of NFκB signaling. It is highly expressed in the postmitotic neurons of the cerebral cortex [98].
To the best of our knowledge, only two proteins have been identified that specifically interact only with the D 2L -R i.e., 29 AA within its ICL3. These proteins are fatty acid-binding protein 3 (FABP3) [99] and Rabaptin-5 interacting protein (Rabex-5) [23]. Fatty acid-binding protein 3 (FABP3), also named the heart-type FABP (H-FAB), is one of the novel 29 AA insert binding protein on the position (G242-V270), which also alters D 2L -R function [99]. D 2L -R, when activated with a ligand, is known to activate the mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) pathways, which are enhanced by FABP3 in FABP3-overexpressed cells, showing that FABP3 enhances D 2L -R signaling [14]. A co-expression study of D 2L -R and D 2S -R with this protein in NG108-15 cells shows overexpression and colocalization of endogenous FABP only with the D 2L -R in the GA and ER but not in the PM [100]. Dysfunction of FABP3 protein binding to D 2L -R was shown in FABP3 KO mice [100], which affects emotional behavior, and is characteristic of neurodegenerative diseases such as schizophrenia and Alzheimer's disorder. These KO mice, which showed altered sensory, motor, and emotional behaviors, also exhibited decreased methamphetamine-induced sensitization and enhanced haloperidol-induced catalepsy due to D 2 -R dysfunction. Impaired FABP brain function was observed as an essential factor in the perturbation of D 2 -R signaling [101]. Rabaptin-5 interacting protein (Rabex-5) was identified in mouse brain lysates as another protein binding the 29 AA of D 2L -R and has been shown to promote the early-endosome formation and Rab5 activation [71]. Both proteins are essential for prolonged D 2L -R mediated ERK signaling.
DRIPs have the propensity to bind to conserved motifs in receptors. For D 1 -R it was shown that the ER-membrane-associated protein DRiP78 binds to a FXXXFXXXF motif in the C-terminus of D 1 -R and other GPCRs. Overexpression or down-modulation of this putative two-TM domain protein leads to ER retention of D 1 -Rs, reduced ligand binding, and impaired kinetics of receptor glycosylation [48]. This mechanism acts as a chaperone and may control PM receptor targeting without traveling to the cell surface.
Some of the DRIPs are also possible "private" chaperones with other functions, escorting proteins for D 2L -R or proteins of the quality-control machinery involved in its retention within intracellular compartments [37] and facilitating receptor cell surface expression by enabling their trafficking to the PM. Pools of intracellular D 1 -R exist in renal tubular cells, and receptor recruitment to the PM is independent of agonist activation elicited by the activation of cell surface receptors and via atrial natriuretic peptide-dependent heterologous activation [102,103].

Interaction with ER-Resident Gatekeeper Proteins
ER gatekeeper proteins tightly control receptor cell-surface export. GTRAP3-18, an integral ER membrane protein, was introduced as a protein both in vivo and in vitro, and is dynamically induced by retinoic acid and inhibits the activity of EAAC1 in a dose dependent manner. GTRAP3-18 forms an oligomeric complex with D 2 -R before exiting the ER, increasing the population of high-mannose oligosaccharide state proteins, and restricting its subcellular localization to the ER [104]. There is evidence that the specific gatekeeper protein PRAF2 binds to subunit GABA 1 of the GABA B receptor and prevents its progression in the biosynthetic pathway [47]. Dupre, J.D. et al. showed that one of the ER-resident proteins, which is known to regulate trafficking via a FXXXFXXXF motif of D-Rs and interact mostly with the Gγ subunit and not Gα or Gβ subunits in HEK-293 cells, is dopamine-receptor interaction protein 78 (DRiP78) [105]. Another ER-gatekeeper candidate of the D 2 -D 3 heterodimer is an activator of G protein signaling 3 (AGS3), which binds to G iα GDP and inhibits GDP dissociation in the prefrontal cortex during late withdrawal from repeated cocaine administration. However, this actual mechanism in D 2 -R signaling is still unknown [106]. In familiar, as well as in sporadic Parkinson's disease, a mutation in the leucine-reach repeat kinase 2 gene (LRRK2) represents the most frequent genetic cause of disease. LRRK2 is a member of the Roco superfamily of proteins, a novel multi-domain family of Ras-like G-proteins, involved in vesicle-mediated transport to the cell membrane. Because LRRK2 could affect D 2 -R turnover by decreasing this rate of trafficking from the GA to the CM, the LRRK2 could have an essential function in one of the possible retention mechanisms [107].

Clinical Relevance
This review examines the role of different types of conserved retention motifs and DRIPs on D 2 -R and roles in routing regulation. The impact of both is of high importance for the physiological functions of D 2L -R and its export trafficking and precise localization in the cell. Defective transport of D 2L -R, as well as many other GPCRs from the ER to the cell surface, is a highly regulated, dynamic process and is associated with the pathogenesis of a variety of human diseases, therefore advances in our understanding of GPCR export. Thus, the secretory pathway and its role in proper cell function are of high importance.
So far, we are not aware of any known mutation in the 29 AA region that would be associated with a disease. No variant of D 2L -R has been linked or associated with schizophrenia, substance abuse, or alcoholism, including the most extensively investigated Ser311Cys polymorphisms of the D 2 -R gene.
In vitro studies showed that the Cys311-type D 2 -R impairs dopamine-induced sequestration, which appears to be consistent with the dopamine hypothesis [108]. A naturally occurring synonymous mutation of the human D 2 -R gene (C957T, P319P) is postulated to correlate with the schizophrenia phenotype, and was shown to markedly change mRNA stability via changes in mRNA secondary structure and reduced dopamine-induced up-regulation of D 2 -R expression [109]. We also conducted a GPCR database (GPCRdb) search to find additional mutants of the D 2 -R and presented them in Table 3. Table 3. A summary of mutations within the ICL3 of the D 2 -R (source: G protein-coupled receptor (GPCR) database (GPCRdb); http://gpcrdb.org/mutations/render). We have not found additional mutations located within the insert (AA 241-270). Further elucidation of the regulatory mechanism underlying GPCR export trafficking may provide an essential foundation for developing new therapeutic strategies in treating diseases. The described differences in the evolutionarily preserved region within the 29 AA insertion in the ICL3 of D 2L -R influences the regulation of D 2L -R cellular trafficking. A thorough search through motifs showed a conserved arginine cluster within the 29-AA insert of ICL3 of the D 2L -R, which appears to be the ER retention signal. Identifying possible candidates for DRIPs may also reveal "private" chaperones, which often display different functions or escort proteins for D 2L -R or proteins of the quality-control machinery that play a role in GPCR retention within intracellular compartments. We speculate that other specific retention mechanisms for D 2L -R exist. Thus, improving our knowledge of the routing regulation of these critical receptors will probably elicit the development of new therapeutic approaches in controlling the targeting of D 2L -R at the PM.