Treatment of Head Louse Infestation with a Novel Mixture Made of Semi-Crystalline Polymers and Plant Extracts: Blind, Randomized, Controlled, Superiority Trial

The rate of head louse infestations is increasing. Most of the neurotoxic treatments are not reliably ovicidal and are faced with genetic resistance. The treatments based on a mechanical mode of action show no sufficient efficacy, transferring the chances of a cure on a tedious combing or leaving the hair fatty. This double-blinded, randomized, controlled, superiority trial evaluates the efficacy and safety of a novel mixture made of semi-crystalline polymers and plant extracts. Forty-five participants received a single application of either 1% Permethrin lotion or the novel lotion, applied for 15 minutes. Untreated and treated nits were collected and placed in an incubator during 10 days. The worst case intent-to-treat analysis found the novel lotion significantly more effective than 1% Permethrin, with 21/22 (95.5%) participants cured and 9/23 (39.1%), respectively (p < 0.0001). Rate of viable nymphs was 0.5 (2.1) for the new lotion and 40.8 (20.4) for the 1% Permethrin. The new lotion cures head louse infestations, offering an effective alternative treatment, with a high efficacy for inhibiting the hatching of eggs. Its physical actions on lice and their eggs should not be affected by resistance to neurotoxic insecticides.


Introduction
Head louse infestations are frequent among children 3-11 years of age and may induce skin irritation, superinfection from scratching, social stigmatization, and psychological distress [1]. Moreover, head louse infestations cause high levels of anxiety among the parents of school-aged children [2]. An increased rate of head louse infestations in recent years has been reported from North and South America, Australia, and in some countries in Europe and Asia [3]. For remedies, various pediculicidal treatments in external use exist across the world. monoterpenes, such as limonene (16.9%), α-phellandrene (16.7%), terpinolene (28.5%), and by α-pyrene (10.5%). More specifically, the resin is composed of a mixture of triterpenes from the α-amyrin (ursane) and β-amyrin (oleane) series and an essential oil, rich in monoterpenes and sesquiterpenes [25,28,29] (see Figure 1).

Figure 1. Chemical information about Protium heptaphyllum.
The resin is often used in cosmetics as a perfume or as a make-up product, forming a kind of lacquer obtained by kneading the resin with a dye and a dissolving grease. The resin, transferred in a lipophilic support, is also suitable for skin care, hair care, and nail care applications. It is a filmforming agent and a tensor.

Materials and Methods
Participants were recruited from a resource-poor community in Fortaleza (capital of Ceará State, Northeast Brazil). Population-based studies in European countries show diverging prevalence, ranging from 1% to 20% [30]. Studies conducted in Northeastern Brazil report a high prevalence of pediculosis capitis among the general population, over 40% [31]. These data represented a strong argument in favor of conducting robust clinical trials to test the effectiveness of a pediculicidal treatment. Participants were recruited via school visits and an oral presentation of the study during a week one month before the start of the clinical trial.

Treatments Used in This Study
In this study, the efficacy and the safety profile of a new anti-lice lotion were compared to a neurotoxic standard treatment in Brazil.
This standard treatment is composed by Permetrin 1% (10 mg) and excipients: carbomer, isopropyl alcohol, methylparaben, propylparaben, cocoamidopropyl betaine, simethicone, sodium hydroxide, sodium lauryl sulfate, propylene glycol, and purified water (manufacturer: CIMED INDÚSTRIA DE MEDICAMENTOS LTDA, Av. Cel. Armando Rubens Storino, 2750 -CEP 37550-000 -Pouso Alegre -MG). The resin is often used in cosmetics as a perfume or as a make-up product, forming a kind of lacquer obtained by kneading the resin with a dye and a dissolving grease. The resin, transferred in a lipophilic support, is also suitable for skin care, hair care, and nail care applications. It is a film-forming agent and a tensor.

Materials and Methods
Participants were recruited from a resource-poor community in Fortaleza (capital of Ceará State, Northeast Brazil). Population-based studies in European countries show diverging prevalence, ranging from 1% to 20% [30]. Studies conducted in Northeastern Brazil report a high prevalence of pediculosis capitis among the general population, over 40% [31]. These data represented a strong argument in favor of conducting robust clinical trials to test the effectiveness of a pediculicidal treatment. Participants were recruited via school visits and an oral presentation of the study during a week one month before the start of the clinical trial.

Treatments Used in This Study
In this study, the efficacy and the safety profile of a new anti-lice lotion were compared to a neurotoxic standard treatment in Brazil.
In the new lotion, the resin is treated. Once the resin is harvested, the essential oil, rich in volatile terpene compounds, is separated by hydrodistillation. So, the resin is deterpenated for a first time, which makes it possible to evaporate the essential oil. Then, the undistilled paste is recovered. The extracted resin presents a crude mixture of pentacyclic triterpenes, represented mostly by amyrine in the α and β form. This mixture is solubilized in alcohol (ethanol) at 96 • with stirring at 40 • C in the ratio 1:3. This process allows a very fine filtering of the dissolved resin. The ethanolic extract is filtered again and concentrated by evaporation of the ethanol under vacuum in order to obtain an extract containing 62.3% of the resin. Finally, the resin is transferred to vegetable oil (mixture of resin and oil), stirred, and decanted for 2 h, which allows the obtention of an optimal transfer rate of 80%. The resin in oil is passed through molecular distillation in order to remove the last volatile compounds. So, it is deterpenated again. The objective of this step is to remove all of the monoterpenes, because the latter have a plasticizing effect, which counteracts the stiffening of the resin. Consequently, there is no more residual monoterpenes (e.g. limonene or linalool) in the mixture, which has no skin-sensitizing, no allergens.

Ethics
A participant information booklet (PIB) was submitted to responding families. Those who wished to enroll came to the school for a detailed oral presentation on pediculosis and the study steps 48 h before the recruitment day (Day 0). The day of recruitment, verbal consent has been asked to check for the presence of live head lice. Each eligible participant had signed consent and assent procedure was followed. A free and enlightened consent form was provided to parents or guardians of children younger than 16 years, and oral assent was asked of children too. A special form has been provided to explain the objective and the course of the study, with a vocabulary adapted to the age of the participants. All participants stated that they understood the purpose and requirements of the investigation before giving written consent. For any child identified as infested by lice, but who was unable to participate in the study for any reason, a standard treatment was offered. All adverse events were monitored during the study, and all changes in concomitant illness and medication were recorded. A completion/withdrawal form was completed at the end of the study period. At the end of the clinical trial, patients not 100% cured were properly treated by a rigorous combing and were provided with a standard treatment. No payment was offered for participation.
The study was conducted under local institutional review and in accordance with the Declaration of Helsinki, the European standard DIN EN ISO 14155:2012-01 (clinical investigation of medical devices for human subjects-good clinical practice), the GCP Regulation, the National Commission for Research Ethics (C.O.N.E.P.), the CNS Resolution number 466 of 2012 and the CNS Operational Norm number 001 of 2013. This is a study of CE-marked medical devices, which were not modified and not used outside of their CE-marked intended purposes and do, therefore, not require ethic approval, which is also applicable for studies in Brazil.

Design
This clinical trial was designed to evaluate the efficacy and confirm the safety of an anti-lice lotion in a single application with a new mechanical mode of action. The product was tested in comparison with a WHO standard treatment acting by neurotoxic mode (Permethrin 1%) in order to discriminate if one treatment was superior to another (superiority test). Our study was double-blinded, and the products looked sufficiently similar to allow double-blinding.
The first objective was to evaluate the efficacy on lice immediately after treatment removal (Day 1), the day after (Day 2), and one week later (Day 8). In vivo data were compared with data from an in vitro study, designed to examine the specific efficacy of the two lotions on eggs hatching, in comparison with a control group consisting of untreated nits. The second objective was to confirm the safety profile of the new product with a dermatologist examination of every participant scalp at each stage of the study.
All enrolled participants ( Figure 2) provided baseline data on age, gender, hair characteristics, siblings, and previous pediculicide use. Three years old was the lower age limit. There was no upper limit.
Cosmetics 2020, 7, x FOR PEER REVIEW 5 of 18 The first objective was to evaluate the efficacy on lice immediately after treatment removal (Day 1), the day after (Day 2), and one week later (Day 8). In vivo data were compared with data from an in vitro study, designed to examine the specific efficacy of the two lotions on eggs hatching, in comparison with a control group consisting of untreated nits. The second objective was to confirm the safety profile of the new product with a dermatologist examination of every participant scalp at each stage of the study.
All enrolled participants ( Figure 2) provided baseline data on age, gender, hair characteristics, siblings, and previous pediculicide use. Three years old was the lower age limit. There was no upper limit. Trained investigators used a visual and manual protocol to examine participants for head lice presence. Infestation degree was determined by the number of alive lice found on each participant after standardized combs strokes, using a fine-toothed plastic detection comb as recommended by Balcioglu et al. [31]. Light infestation was defined as 1 louse to 3 lice found after 3 comb strokes, moderate infestation as 4 to 6 lice found after 3 comb strokes, and heavy infestation as more than 7 lice found after 3 comb strokes.
Participants who, after examination, were confirmed to be infested by lice and who had at least 5 visible nits found in the part of the hair closer than 1.0 cm from the skull were eligible. Participants were also required to confirm their availability for the duration of the study (8 days following the treatment) in order to be included in the study. Exclusion criteria were known to be sensitivity to any of the ingredients; secondary bacterial infestation of the scalp (e.g., impetigo) or presence of any disease of the scalp (e.g., psoriasis or eczema); pregnant or breast-feeding; participation in another Trained investigators used a visual and manual protocol to examine participants for head lice presence. Infestation degree was determined by the number of alive lice found on each participant after standardized combs strokes, using a fine-toothed plastic detection comb as recommended by Balcioglu et al. [31]. Light infestation was defined as 1 louse to 3 lice found after 3 comb strokes, moderate infestation as 4 to 6 lice found after 3 comb strokes, and heavy infestation as more than 7 lice found after 3 comb strokes.
Participants who, after examination, were confirmed to be infested by lice and who had at least 5 visible nits found in the part of the hair closer than 1.0 cm from the skull were eligible. Participants were also required to confirm their availability for the duration of the study (8 days following the Cosmetics 2020, 7, 25 6 of 18 treatment) in order to be included in the study. Exclusion criteria were known to be sensitivity to any of the ingredients; secondary bacterial infestation of the scalp (e.g., impetigo) or presence of any disease of the scalp (e.g., psoriasis or eczema); pregnant or breast-feeding; participation in another clinical trial within 1 month before entry to this study; use of hair bleach, color, or permanent wave products within the previous four weeks; use of any type of pediculicide during the 15 days before the initiation of the treatment, and treatment by antibiotics.
All procedures, including preliminary tests, were conducted at school. Experimental and positive products were supplied in 120 mL bottles. The day of the intervention (Day 1), a standard allergy test was performed on the right and left arms on each participant. Experimental and positive products were left in contact to the skin of the elbow during 20 minutes. Then, the participants were randomly allocated into group "treatment A" (new product) or into group "treatment B" (Permethrin 1% lotion). Assignment was consigned on a specific form by an independent observer, in case of adverse event. Patients, practitioners, and assessors were blind to the treatment group.
Each treatment was applied at the same time in two separate rooms. Members of the school staff applied the treatments under supervision of members of the study team, after a good understanding of each practitioner was checked. Each product was applied on dry hair, using enough to thoroughly moisten the hair and scalp. Treatments were applied to the full hair length and left for 15 minutes. After the application time, the hair of participants was wetted (water spray) during one minute. Finally, each treatment was removed using a normal shampoo, and only 15-20 comb strokes were given on half the head of each participant, in order to examine lice conditions.
To provide diagnostic snapshot data of the status of infestation, lice found were removed and fixed to the case record (participant's Case Report Form -CRF) using clear tape. Each louse was taped, mentioning the development stage (immature or adult) and state (live or dead). Death was appreciated using the observation grid established by the Department of Parasitology of the University of Tours (France). Only K-D3+ (no visible movement, except some sporadic intestinal movements) and K-D4+ (no visible movement, so external as internal, except a tiny contraction of the digestive tract after stimulation by a clamp) were registered as dead, whereas lice presenting strong anomalies of movements compared as still capable of turning around when on their backs were considered as alive. Lice still capable of some movements internal or external and turning around with difficulty when on their backs were also considered as alive.
In order to determine the cure rate, a meticulous combing was performed on Day 2 (one day after treatment). Examinations were made using the comb across the whole scalp. At Day 8 (final assessment), a more extensive examination was made to try to ensure no lice were present. At each assessment, the participant's hair and scalp were visually examined and combed with a head louse comb (plastic teeth), and, as at Day 1, any louse found were taped (with clear tape) into the participant's CRF. All participants were examined at days 1, 2, and 8, unless they were lost to follow up.
Criterion specified for a cure was "no living lice at D1 and D2 and no living immature at D8". We also performed analyses on the following separate criteria: "absence of alive lice on D8", "absence of living immature on D8", "absence of alive lice and alive immature on day 2", and "absence of alive lice and immature lice on D1". Criterion for exogenous reinfestation was "no alive lice found after the treatments at day 1 and day 2; and, on day 8, no more than four adult lice and no nymph found during combing".
Considering that ovicidal efficacy outcomes of pediculicides are often not reliable because they are only assessed indirectly (e.g., observing the number of lice at premature stages found on a head several day after treatment, indicating inefficacy on nits), we designed an in vitro study in order to alleviate this difficulty. To examine the specific efficacy of the two lotions on the eggs, several nits (depending on the infestation degree) were collected on each participant before treatment (during the diagnosis) and then put in an incubator at 32 ± 1 • C and 54% relative humidity, in order to be the control group. Several nits (depending on the infestation degree) were collected on each participant after treatment removal at day 1. To respect the parsimony principle, only visible full nits were taken by cutting a Cosmetics 2020, 7, 25 7 of 18 hair lock. Each day, during ten days, the hatching rate was examined (counting hatched eggs and determining the viability of nymphs by their survival 3 h after hatching) in the two treatment groups and compared with the control group. The number of hatches was clearly taped in a specific file.

Statistical Analysis
For sample size determination, we anticipated that there was likely to be a disparity in efficacy between the new semi-crystalline lotion and Permethrin 1% lotion, based on previous data (in vitro studies for the experimental product and data available in the literature for permethrin). For a randomize-by-individual approach, a sample size of 17 per group was estimated to have at least 80% power with a type I error rate (one-sided) of 2.5% to detect a difference of 38% between the success rates for the experimental lotion and 1% Permethrin, based on a 98% success rate for the experimental lotion (in vitro test) and evidence suggesting lower success rates with 1% Permethrin (mean of 62.5% according to available data) [33][34][35][36][37][38][39][40][41][42][43].
Recoding of inclusion criteria was: Comparisons of the two populations were performed by a Student's t-test or Mann-Whitney test for continuous variables (the normality of the variables was tested by a Shapiro-Wilk test) and by chi-square tests or Fisher for categorical variables.
For in vivo outcomes, the endpoints are presented as numbers (proportions) and the difference in rates (A Treatment-B Treatment) was estimated by a 95% bilateral confidence interval. It was possible to conclude that the superiority of treatment A vs. treatment B if the lower limit of the confidence interval was strictly positive.
For in vitro outcomes, the evolution over time of the cumulative hatching rate and the cumulative rate of viable nymphs were investigated by a generalized linear model with repeated measures (PROC GLIMMIX), including the time from the collection, and with an autoregression(1) covariance matrix structure for repeated measurement. The odds-ratio of treatment A vs. treatment B for hatching and viable nymphs was estimated by a bilateral confidence interval of 95%, and we could conclude to the superiority of treatment A vs. treatment B if the upper bound of the confidence interval was strictly less than 1. The type I error rate was set at 2.5% (one-sided) for the superiority analysis and 5% (two-sided) for other analyses. The analyses were performed by intent-to-treat; per protocol analysis was also conducted to supplement the results. All analyses were performed with SAS software (SAS v9.4 SAS Institute).

Results
Our study was carried out in August 2016. Forty-five young people aged 6 to 10 years agreed to take part and met the eligibility criteria. The two groups were similar in age, sex, intensity of infestation, and hair length and degree of curl. No adverse events occurred. Before treatment, 13 (29%) participants had heavy louse infestations, 12 (27%) medium infestations, and 20 (44%) light infestations (Table 1). According to the weighing of the bottles after application, 67mL of product were used on average, with a maximum of 92mL for the experimental product and 90mL for the treatment with Permethrin 1%. The primary analysis was an intent-to-treat and involved all patients who were randomly assigned (45 participants). The secondary analysis was per-protocol. One participant in the treatment B group was lost to follow-up at Day 2 and at Day 8. One patient was considered a protocol violator in the treatment B group; consequently, forty-three patients remained for the per protocol analyses.
We performed analyses on each different healing criterion: absence of live lice on D8, absence of living immature on D8, absence of live lice and live immature on D2, absence of live lice and live immature on D1 and, then, absence of live lice on D1 and on D2 and absence of live immature on D8. We also performed specific analyses on the hatching rate of nits and the rate of viable immatures.
3.1.1. Results for the Criterion "Absence of Live Lice on D8" Four children with exogenous re-infestations according to the protocol definition were identified #19, #22, #27 (treatment A), and #52 (treatment B). They were considered either as successes (intent-to-treat (ITT) with re-infestations) or as failures for a sensitivity analysis (ITT, without re-infestations).
In ITT, regarding the "worst case" and under the efficacy criterion "no living lice at D8", data show a 95.5% success for the new pediculicide against 52% for the standard treatment (Permethrin 1%). The difference (IC95%) between the two treatments is 43.3 (16.0; 67.3), with p-value A vs. B of 0.0005. For per protocol (PP), results are the same for the new treatment and 47.6% for the Permethrin 1% lotion ( Table 2).
The multivariate binary logistic regressions show an adjusted treatment effect whatever the set of analyses (Table 3): treatment A multiplies the chances of recovery by 11, 42, or 53 depending on the samples considered, regardless of the degree of hair infestation. Results also highlight an effect of the degree of infestation: a heavy infestation divides the risk of healing by 1/0.077 = 13, 1/0.062 = 16, or 1/0.059 = 17, depending on the samples considered, regardless of the treatment. No interaction between the treatment and the degree of infestation has been demonstrated, which means that it has not been possible to show that the effectiveness of a treatment varies with the degree of infestation. The results show that treatment A cured 100% of the children. It was not possible to build logistic regression models with this treatment, since all the children treated with A were successful. In ITT regarding the "worst case" and under the efficacy criterion "no living immature lice at D8", data showed 100% efficacy for the new treatment and 39.1% for the standard treatment, with difference between the two products of 60.9 (35.8; 80.9), p sup A vs. B < 0.0001 (Table 4). Since we are at D2 and at D1, the notion of exogenous re-infestation is not to be considered. The results show 21/22 (95.5%) cured for treatment A, 12/23 (52.2%) and 11/22 (50/0%) for treatment B at Day 2 and Day 1, respectively ( Table 5). The results are the same for both strategies. In ITT, regarding the "worst case" and under the efficacy criterion "no living lice at D1 and D2 and no living immature at D8", data show 95.5% success for the new pediculicide and 39.1% for the 1% Permethrin, with a difference of 56.3% (30.7; 77.6), p sup A vs. B < 0.0001 (Table 6).  Table 7 summarizes the results for each criterion, for the primary endpoint and secondary endpoints in vivo, intent-to-treat, and per-protocol analyses.

Additional Analyses
We noticed that the early results were already very satisfactory for treatment A. We crossed the results "absence of live lice and live immature D1" with "absence of live lice on D1 and D2 and absence of live immatures on D8". The results were as follows (Tables 8 and 9): Thus, of the 21 children having neither immature living nor louse living on D1, 21 (therefore, 100%) were considered as cured on D8 (absence of live lice on D1 and D2 and absence of living immature on D8).
Among the 12 children with neither immature living nor louse living on D1, 9 (or 75%) were considered to be cured on D8 (absence of live lice on D1 and D2 and absence of living immatures on D8). We can conclude that the rate of children cured on D8 differs depending on the treatment (p = 0.04). The results were exactly the same with D2.

Ovicidal Efficacy of the Two Treatments, in Vitro Part of the Study
The outcome was the frequency of ovicidal failure, measured by the percentage of hatched eggs in the incubator and measured by the imago viability at three hours after hatching.

Comparison of Cumulative Hatching Rates on D10
If we look at D10 and compare the rates (total number of outbreaks/total number removed) of children with treatment (A vs. B), we obtain the following results: cumulative hatching rate at D10 is 1.8 (6.6) for the new pediculicide and 53.7 (26.5) for the 1% Permethrin. Rate of viable nymphs is 0.5 (2.1) for the new pediculicide and 40.8 (20.4) for the 1% Permethrin. Thus, on D10, the risk of hatching with treatment A is multiplied by 0.017 (divided by 60). In the same way, the risk of viable hatching with treatment A is multiplied by 0.007 (divided by 140) (Table 10).

Comparison of Cumulative Hatching Rates from D2 to D10
Including in the analysis, the observations of children with treatments from D2 to D10, we highlight a treatment effect and a time effect (Table 11; Figure 3; Figure 4), but we cannot include an interaction between the two factors, because the model does not converge. The risk of hatching with treatment A is divided by 1/0.023 (a little more than 40), whatever the time considered. The risk of viable hatching with treatment A is divided by 1/0.010 (100), whatever the time considered. interaction between the two factors, because the model does not converge. The risk of hatching with treatment A is divided by 1/0.023 (a little more than 40), whatever the time considered. The risk of viable hatching with treatment A is divided by 1/0.010 (100), whatever the time considered.

Discussion
This study has shown that a single application of the new lotion made by semi-crystalline polymers and plant extracts is effective to eliminate in 15 min head louse infestation and to inhibit eggs hatching. However, as with all treatments, it is possible to miss some louse eggs during treatment, requiring post-treatment vigilance for emerging nymphs more than seven days after treatment. In this study, four infestations in the ITT group without nymphs (one in group B and three in group A) were attributable to reinfestation from contacts either within the family or the local community.
As the participants went back home each night, a high risk of reinfestation after a cure was expected. Knowledge of family circumstances (siblings) helped us to identify some reinfestation risks, but for statistical purposes, we specified criteria for exogenous reinfestation: no live lice found after the treatments at days 1 and day 2 and, on day 8, no more than four adult lice and no nymphs found during combing. We acknowledge these criteria could give false outcomes either way, but we believe they addressed the issue of reinfestation without presenting an unreasonably optimistic view of the product efficacy. The presence of small lice (nymphs) was evidence that not all the eggs were killed by the treatment.
Furthermore, in vitro analysis of the hatching rate of lice eggs helped us determine whether the presence of any louse was due to surviving phenomenon or due to the survival of any egg, from which nymph was able to emerge. In vitro experiment was useful to determine the specific efficacy of each treatment on the hatching rate of eggs in order to maximize the power of the study. This in vitro study had a strong interest in order to discriminate between a treatment failure and a recontamination by the environment and to ensure that the protocol was not broken by families at home. An occasional adult louse found after the initial infestation was clearly the indicator of an exogenous reinfestation. Criteria for a cure of infestation was defined with the most demanding criteria, as no evidence of living head lice after treatment at day 1 and no evidence of living head lice at day 2 and no evidence of living immature head lice at day 8. Any participant not fitting the cure criteria or exogenous reinfestation criteria after the cure was categorized as a treatment failure.
Regarding the study protocol of intervention, we chose to harmonize the application for the two products tested, in order to optimize the comparison conditions and to permit a double-blind trial. We have taken as standard the recommendations for use of the manufacturer of the new treatment, because the permethrin is not known for basing its mode of action and, therefore, its efficacy on the mechanical manipulations linked to the product application, contrary to the technology and the mechanical mode of action claimed for the new lotion (Deparaz®ParaPio®). Contrary to the manufacturer's recommendations for the Permethrin 1% (Pediletan®), no rigorous combing was performed before treatment removal. Furthermore, for the two treatments, no rigorous combing was made immediately after treatment removal, because this modality would lead to a serious bias regarding the efficacy measure of each lotion themselves.
The efficiency of this new lotion seems to consist in the plant's resin Protium heptaphyllum mechanical action, easily distributed along the hair and on the parasites thanks to Eur-Amid N2®, by decreasing the surface tension. Citrus aurantium dulcis peel wax consists of a complex mixture of special esters (ca. 50%-65%, C44-C58), free fatty acids (6%-15%, C14-C22), hydrocarbons (8%-15%, C21-C33), free phytosterols (ca. 4%-8%), and free alcohols. It is used to stabilize the mixture. Eur-Amid N2®and phytosterols help to dissolve the epicuticular waxes that cover the shells of the parasites. As a result, the chitin becomes accessible to the resin. We observed that the Protium heptaphyllum resin, usually used as a film-forming agent in cosmetics, seems to have some specific affinities for the chitin (polysaccharides) of the parasites. There is a phenomenon of coating of the shell of lice and their nits.
The liquefied resin has the property to stiffen under contact with aqueous polar environment. That is the reason why the mixture has to be put in contact with water after the exposure time. In contact with water, the resin hardens and shrinks by a few microns. This phenomenon seems to cause cracking of the rigid shell of the parasites. This mechanism leads to a breakdown of external defenses and normal biological functions of lice and nits. It seems to happen as a perforation and dislocation of the rigid shell and the paws of lice ( Figures 5 and 6), and the operculum of the nits is covered with resin, which seems to destroy its opening-closing mechanism (Figures 7-9). Under these conditions, lice are paralyzed, unable to move, feed, and reproduce. So, parasites are easily eliminated by the shampoo, because they cannot hang on to the hair anymore, and nits still hanging in the hair are no longer viable because the exchanges between them and their environment were disrupted by the destruction of the protective articulated operculum.     Cosmetics 2020, 7, x FOR PEER REVIEW 15 of 18 with resin, which seems to destroy its opening-closing mechanism (Figure 7, Figure 8 and Figure 9). Under these conditions, lice are paralyzed, unable to move, feed, and reproduce. So, parasites are easily eliminated by the shampoo, because they cannot hang on to the hair anymore, and nits still hanging in the hair are no longer viable because the exchanges between them and their environment were disrupted by the destruction of the protective articulated operculum.    Cosmetics 2020, 7, x FOR PEER REVIEW 15 of 18 with resin, which seems to destroy its opening-closing mechanism (Figure 7, Figure 8 and Figure 9). Under these conditions, lice are paralyzed, unable to move, feed, and reproduce. So, parasites are easily eliminated by the shampoo, because they cannot hang on to the hair anymore, and nits still hanging in the hair are no longer viable because the exchanges between them and their environment were disrupted by the destruction of the protective articulated operculum.       This product does not damage the hair and the scalp. After shampoo, the hair benefits from the resin residue, playing the role of conditioner. Furthermore, grapeseed oil nourishes the hair thanks to the presence of fatty acids such as Omega-6; orange peel wax is often used as hair care in cosmetics, and Eur-Amid N2® is a mild nonionic surfactant used for its good foaming and viscosity-building properties, usually employed in shampoos. These surfactants are designed for imparting smoothness to skin and appreciated for children who present itching scalps due to repeated lice bites. Other benefits are the potential reduction of the irritation of anionic surfactants to skin and eyes, being a safer liquid alternative to cocamide DEA and having a vegetable origin. Finally, the antiinflammatory properties of amyrin can also soothe itchy, irritated scalps in children.

Conclusion
Considering the results provided by this study for the killing of lice and eggs rates and regarding its directions for use (no necessary combing), the new lotion could be an interesting way to eradicate lice from the communities. It is possible that this treatment can be used frequently, because the parasites are killed mechanically and not by some neurotoxic agents. Lice may not be able to develop genetic resistance to this type of mechanical action treatment. This approach seems to be a promising alternative also considering its natural composition and the anti-inflammatory properties of some of its components.  This product does not damage the hair and the scalp. After shampoo, the hair benefits from the resin residue, playing the role of conditioner. Furthermore, grapeseed oil nourishes the hair thanks to the presence of fatty acids such as Omega-6; orange peel wax is often used as hair care in cosmetics, and Eur-Amid N2®is a mild nonionic surfactant used for its good foaming and viscosity-building properties, usually employed in shampoos. These surfactants are designed for imparting smoothness to skin and appreciated for children who present itching scalps due to repeated lice bites. Other benefits are the potential reduction of the irritation of anionic surfactants to skin and eyes, being a safer liquid alternative to cocamide DEA and having a vegetable origin. Finally, the anti-inflammatory properties of amyrin can also soothe itchy, irritated scalps in children.

Conclusions
Considering the results provided by this study for the killing of lice and eggs rates and regarding its directions for use (no necessary combing), the new lotion could be an interesting way to eradicate lice from the communities. It is possible that this treatment can be used frequently, because the parasites are killed mechanically and not by some neurotoxic agents. Lice may not be able to develop genetic resistance to this type of mechanical action treatment. This approach seems to be a promising alternative also considering its natural composition and the anti-inflammatory properties of some of its components.