Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals, Ejaculates and Seminal Plasma Collection
2.2. Cytokine Measurements in Seminal Plasma Samples
2.3. Experimental Design
2.3.1. Experiment 1: Interval Ejaculation–Seminal Plasma Harvesting
2.3.2. Experiment 2: Storage and Transport Conditions for Seminal Plasma before Analysis
2.3.3. Experiment 3: Effects of Frozen Storage Time on Seminal Plasma Cytokine Contents
2.4. Statistical Analysis
3. Results
3.1. Experiment 1: Interval Ejaculation–Seminal Plasma Harvesting
3.2. Experiment 2: Storage and Transport Conditions for Seminal Plasma before Analysis
3.3. Experiment 3: Effects of Frozen Storage Time on Seminal Plasma Cytokine Contents
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cytokine (pg/mL) | Median (25th, 75th Percentiles) | Range |
---|---|---|
GM-CSF | 31.00 (27.50, 34.88) | 27.50 |
IFNγ | 1317.00 (1073, 1496) | 667.50 |
IL-1α | 10.75 (4.00, 25.38) | 37.50 |
IL-1β | 18.75 (15.63, 99.50) | 480.50 |
IL-1ra | 626.30 (293.90, 1763.00) | 3621.00 |
IL-2 | 5.25 (3.62, 9.00) | 11.00 |
IL-4 | 21.75 (18.63, 23.50) | 9.50 |
IL-6 | 83.25 (64.25, 151.90) | 347.50 |
IL-8 | 32.75 (22.50, 54.88) | 99.80 |
IL-10 | 9.50 (8.62, 13.63) | 10.50 |
IL-12 | 3.00 (3.00, 4.25) | 68.20 |
IL-18 | 20.50 (4.25, 77.63) | 195.00 |
TNFα | 18.25 (13.75, 23.50) | 19.00 |
Cytokine | Spearman Correlation (p-Value) | ||||||
---|---|---|---|---|---|---|---|
Experiment 1 | Experiment 2 | Experiment 3 | |||||
2 h vs. 0 h | 24 h vs. 0 h | 5 °C vs. Fresh | −20 °C vs. Fresh | −80 °C vs. Fresh | −20 °C vs. Fresh | −80 °C vs. Fresh | |
GM-CSF | 0.83 (<0.001) | 0.70 (0.001) | 0.89 (<0.001) | 0.74 (<0.001) | 0.81 (<0.001) | 0.48 (0.032) | 0.56 (0.010) |
IFNγ | 0.62 (0.004) | 0.74 (0.002) | 0.79 (<0.001) | 0.56 (ns) | 0.72 (0.006) | 0.32 (ns) | 0.62 (0.003) |
IL-1α | 0.98 (<0.001) | 0.99 (<0.00) | 0.90 (<0.001) | 0.90 (<0.001) | 0.88 (<0.001) | 0.81 (<0.001) | 0.85 (<0.001) |
IL-1β | 0.86 (<0.001) | 0.81 (<0.00) | 0.92 (<0.001) | 0.93 (<0.001) | 0.95 (<0.001) | 0.93 (<0.001) | 0.85 (<0.001) |
IL-1ra | 0.97 (<0.001) | 0.99 (<0.00) | 0.96 (<0.001) | 0.96 (<0.001) | 0.97 (<0.001) | 0.94 (<0.001) | 0.96 (<0.001) |
IL-2 | 0.88 (<0.001) | 0.89 (<0.00) | 0.70 (<0.001) | 0.71 (0.006) | 0.70 (0.003) | 0.57 (0.008) | 0.83 (<0.001) |
IL-4 | 0.79 (<0.001) | 0.76 (<0.00) | 0.81 (<0.001) | 0.73 (0.011) | 0.70 (<0.011) | 0.60 (0.005) | 0.72 (<0.001) |
IL-6 | 0.95 (<0.001) | 0.91 (<0.00) | 0.96 (<0.001) | 0.97 (<0.001) | 0.96 (<0.001) | 0.98 (<0.001) | 0.97 (<0.001) |
IL-8 | 0.68 (<0.001) | 0.75 (<0.00) | 0.83 (<0.001) | 0.91 (<0.001) | 0.94 (<0.001) | 0.77 (<0.001) | 0.86 (<0.001) |
IL-10 | 0.82 (<0.001) | 0.84 (<0.00) | 0.66 (0.003) | 0.84 (0.010) | 0.71 (<0.010) | 0.53 (0.016) | 0.65 (0.002) |
IL-12 | 0.70 (<0.001) | 0.90 (<0.00) | 0.86 (<0.001) | 0.84 (<0.001) | 0.83 (<0.001) | 0.34 (ns) | 0.84 (<0.001) |
IL-18 | 0.95 (<0.001) | 0.93 (<0.00) | 0.82 (<0.001) | 0.78 (<0.001) | 0.80 (<0.001) | 0.63 (0.003) | 0.86 (<0.001) |
TNFα | 0.92 (<0.001) | 0.93 (<0.00) | 0.83 (<0.001) | 0.71 (0.009) | 0.72 (<0.001) | 0.40 (ns) | 0.60 (0.005) |
Cytokine (pg/mL) | Median (25th, 75th Percentiles) | Range |
---|---|---|
GM-CSF | 44.34 (40.17, 54.42) | 34.66 |
IFNγ | 2457.00 (2227.00, 2699.00) | 1223.00 |
IL-1α | 4.83 (4.16, 7.08) | 12.00 |
IL-1β | 16.17 (14.83, 19.58) | 19.67 |
IL-1ra | 147.80 (57.42, 288,3) | 449.00 |
IL-2 | 10.33 (7.58, 11.17) | 7.00 |
IL-4 | 32.67 (29.34, 34.75) | 15.67 |
IL-6 | 82.17 (47.58, 225.20) | 290.00 |
IL-8 | 34.00 (29.50, 53.50) | 63.33 |
IL-10 | 13.67 (12.00, 14.42) | 7.67 |
IL-12 | 6.50 (4.91, 7.00) | 4.34 |
IL-18 | 15.34 (9.25, 27.00) | 32.67 |
TNFα | 23.67 (20.33, 25.00) | 11.33 |
Cytokine (pg/mL) | Median (25th, 75th Percentiles) | Range |
---|---|---|
GM-CSF | 39.67 (36.67, 47.33) | 42.33 |
IFNγ | 2004.00 (1881.00, 2219.00) | 1097 |
IL-1α | 4.00 (4.00, 5.75) | 26.67 |
IL-1β | 17.33 (16.00, 22.00) | 38.33 |
IL-1ra | 138.00 (51.25, 225.20) | 757.00 |
IL-2 | 7.67 (6.00, 8.00) | 13.00 |
IL-4 | 29.33 (27.00, 31.67) | 14.00 |
IL-6 | 77.17 (40.33, 209.10) | 278.70 |
IL-8 | 37.17 (25.83, 56.67) | 342.00 |
IL-10 | 11.67 (10.50, 12.58) | 9.67 |
IL-12 | 5.00 (4.08, 5.67) | 14.33 |
IL-18 | 15.33 (10.08, 27.00) | 62.67 |
TNFα | 20.50 (18.67, 21.59) | 32.34 |
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Padilla, L.; Barranco, I.; Parrilla, I.; Lucas, X.; Rodriguez-Martinez, H.; Roca, J. Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage. Biology 2020, 9, 276. https://doi.org/10.3390/biology9090276
Padilla L, Barranco I, Parrilla I, Lucas X, Rodriguez-Martinez H, Roca J. Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage. Biology. 2020; 9(9):276. https://doi.org/10.3390/biology9090276
Chicago/Turabian StylePadilla, Lorena, Isabel Barranco, Inmaculada Parrilla, Xiomara Lucas, Heriberto Rodriguez-Martinez, and Jordi Roca. 2020. "Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage" Biology 9, no. 9: 276. https://doi.org/10.3390/biology9090276