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Article

A Simplified Iohexol-Based Method to Measure Renal Function in Sheep Models of Renal Disease

1
Department of Nephrology and Hypertension, IIS-Fundacion Jimenez Diaz, 28040 Madrid, Spain
2
Dpto. de Produccion y Sanidad Animal, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, C/Tirant lo Blanc, 7, 46115 Alfara del Patriarca Valencia, Spain
3
Research Unit, Hospital Universitario de Canarias, 38320 La Laguna, Tenerife, Spain
4
Internal Medicine Department, Hospital Universitario de Canarias, 38320 La Laguna, Tenerife, Spain
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Laboratorio Función Renal, Universidad de La Laguna, 38320 La Laguna, Tenerife, Spain
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Department of Pharmacy, University of Oslo, 0316 Oslo, Norway
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Department of Transplantation Medicine, Oslo University Hospital Rikshospitalet, 0424 Oslo, Norway
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Avda Pta. de Hierro s/n, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Biology 2020, 9(9), 259; https://doi.org/10.3390/biology9090259
Received: 21 July 2020 / Revised: 25 August 2020 / Accepted: 26 August 2020 / Published: 31 August 2020
Sheep are highly adequate models for human renal diseases because of their many similarities in the histology and physiology of kidney and pathogenesis of kidney diseases. However, the lack of a simple method to measure glomerular filtration rate (GFR) limits its use as a model of renal diseases. Hence, we aimed to develop a simple method to measure GFR based on the plasma clearance of iohexol by assessing different pharmacokinetic models: (a) CL2: two-compartment (samples from 15 to 420 min; reference method); (b) CL1: one-compartment (samples from 60 to 420 min); (c) CLlf: CL1 adjusted by a correction formula and (d) SM: simplified CL2 (15 to 300 min). Specific statistics of agreement were used to test the models against CL2. The agreement between CL1 and CL2 was low, but both CL1f and SM showed excellent agreement with CL2, as indicated by a total deviation index of ~5–6%, a concordance correlation of 0.98–0.99% and a coverage probability of 99–100%, respectively. Hence, the SM approach is preferable due to a reduced number of samples and shorter duration of the procedure; two points that improve animal management and welfare. View Full-Text
Keywords: renal function; iohexol plasma clearance; sheep model renal function; iohexol plasma clearance; sheep model
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MDPI and ACS Style

Luis-Lima, S.; Mas-Sanmartin, C.; Rodríguez-Rodríguez, A.E.; Porrini, E.; Ortiz, A.; Gaspari, F.; Diaz-Martin, L.; Åsberg, A.; Jenssen, T.; Jiménez-Sosa, A.; Martinez-Ros, P.; Gonzalez-Bulnes, A. A Simplified Iohexol-Based Method to Measure Renal Function in Sheep Models of Renal Disease. Biology 2020, 9, 259. https://doi.org/10.3390/biology9090259

AMA Style

Luis-Lima S, Mas-Sanmartin C, Rodríguez-Rodríguez AE, Porrini E, Ortiz A, Gaspari F, Diaz-Martin L, Åsberg A, Jenssen T, Jiménez-Sosa A, Martinez-Ros P, Gonzalez-Bulnes A. A Simplified Iohexol-Based Method to Measure Renal Function in Sheep Models of Renal Disease. Biology. 2020; 9(9):259. https://doi.org/10.3390/biology9090259

Chicago/Turabian Style

Luis-Lima, Sergio, Carolina Mas-Sanmartin, Ana E. Rodríguez-Rodríguez, Esteban Porrini, Alberto Ortiz, Flavio Gaspari, Laura Diaz-Martin, Anders Åsberg, Trond Jenssen, Alejandro Jiménez-Sosa, Paula Martinez-Ros, and Antonio Gonzalez-Bulnes. 2020. "A Simplified Iohexol-Based Method to Measure Renal Function in Sheep Models of Renal Disease" Biology 9, no. 9: 259. https://doi.org/10.3390/biology9090259

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