Effects of Chitosan-Based Coatings Enriched with Cinnamaldehyde on Mandarin Fruit cv. Ponkan during Room-Temperature Storage

: The current work aimed to evaluate the efﬁcacy of four coating formulations—chitosan coating (CH), 0.5% cinnamaldehyde + chitosan (0.5% CI–CH), 1.0% cinnamaldehyde + chitosan (1.0% CI–CH), and 1.5% cinnamaldehyde + chitosan (1.5% CI–CH)—on fresh mandarin fruit cv. Ponkan quality maintenance (weight loss, decay rate, total soluble solids, titratable acidity, vitamin C, color index, malondialdehyde, and antioxidant activity) over 100 days of storage at 20 ◦ C. Compared to the control, chitosan treatment effectively reduced the decay and weight loss rates of mandarin fruit cv. Ponkan during storage at room temperature, delayed the decline of nutritional quality in fruits, increased the antioxidant capacity, and inhibited the accumulation of malondialdehyde (MDA). In comparison to chitosan coating, 1.5% CI–CH did not improve the fruit storage effect, but inhibited the normal color change of fruits and increased the accumulation of MDA. Both 0.5% CI–CH and 1.0% CI–CH effectively reduced the rate of fruit decay, improved the quality of fruits after harvest, and delayed fruit aging. Our study suggests that 0.5% CI–CH and 1.0% CI–CH might be good formulations for maintaining the quality of mandarin fruit cv. Ponkan during room-temperature storage.


Introduction
Citrus is the most important commodity fruit in the world and the most widely cultivated fruit tree type in the subtropics of China.The genus citrus includes orange, grapefruit, kumquat, lemon, and many others, including various varieties in each species [1].Mandarin fruit cv.Ponkan (Citrus reticulata Blanco cv.Ponkan) is a widely cultivated citrus variety in southern China.Its fruit matures in mid-November to late December.The skin of mature Ponkan is orange and easy to peel, and the pulp is crisp and juicy, with a strong flavor [2].In actual production, based on cost and technical constraints, the fruit growers usually store the picked Ponkan fruits at room temperature.However, Ponkan has a thin skin, and the quality of the fruit during storage at room temperature is highly prone to deterioration and decay over time [3].Improving the storage performance and reducing fruit decay during storage and transportation are major problems for the citrus industry.At present, the postharvest preservation technology for citrus mainly includes low-temperature storage, coating,

Decay Rate
To determine the rate of decay, 300 fruits were processed and divided into three replicates.The number of rotting fruits was counted every 20 days.The following equation was used to calculate the decay rate of fruits: decay rate = rotting fruits total fruits × 100% 2.4.3.Total Soluble Solids, Titratable Acidity, and Vitamin C A homogeneous sample of 10 fruits was prepared for each treatment to measure the content of total soluble solids (TSS), titratable acidity (TA), and vitamin C (Vc).TSS content was measured with an RA-250WE digital brix meter (Kyoto, Tokyo, Japan).TA content was determined by the acid-base titration method.Vc content was determined by 2-6-dichloroindophenol titration [27].

Color Index
Ten fruits from each treatment time were used for the determination of surface color change.This was carried out with a chromameter (CR-400, Konica Minolta, Osaka, Japan) at four evenly distributed equatorial sites of each fruit [28].Citrus color index was calculated according to methods reported before [29].
CCI = 1000 × a * /(L * ×b * ) 2.4.5.Content of Malondialdehyde of Fruit Peel MDA content was determined by the thiobarbituric acid colorimetric method [30].First, 1 g of the sample was weighed, and after thorough grinding, 8 mL of 10% trichloroacetic acid was added and centrifuged at 10,000× g for 15 min.Then 2 mL of the supernatant was placed into the test tube, 2 mL of 0.6% thiobarbituric acid was added, water was boiled for 15 min, the mixture was rapidly cooled and centrifuged, and the absorbance of the supernatant was measured at 450, 532, and 600 nm wavelengths.
CAT activity was determined by the ultraviolet absorption method.The reaction mixture consisted of 1.0 mL of 0.3% hydrogen peroxide, 1.9 mL of buffered substrate (0.05 M sodium phosphate buffer, pH 7.0), and 0.1 mL of the enzyme solution.Enzyme activity was measured at 240 nm by using a spectrophotometer (UV-2600, Shimadzu, Kyoto, Japan).The increase in absorbance was recorded every 30 s for 3 min.One unit of POD activity was defined as an increase in absorbance at 0.001/min.
POD activity was determined by the guaiacol method.The reaction mixture consisted of 0.1 mL of 0.4% guaiacol, 0.1 mL of 0.46% H 2 O 2 , 2 mL of buffered substrate (0.05 M sodium phosphate buffer, pH 7.0), and 1 mL of the enzyme solution.Enzyme activity was measured at 470 nm by using a spectrophotometer (UV-2600, Shimadzu, Kyoto, Japan).The increase in absorbance was recorded every 30 s for 3 min.One unit of POD activity was defined as an increase in absorbance at 0.001/min.SOD activity was determined by the nitroblue tetrazolium photoreduction method.First, 0.1 mL of the enzyme solution was added to 5 mL of the reaction medium (containing 15 mmol/L Met, 65 µmol/L NBT, 2.0 µmol/L riboflavin, and 0.1 mmol/L EDTA, prepared with 50 mmol/L pH 7.8 phosphate buffer).After 15 min of illumination (4000 lx), the absorbance was measured immediately at 240 nm using a spectrophotometer (UV-VIS 04834, Shimadzu, Kyoto, Japan), and the buffer solution was used as a blank.The enzyme activity was determined by inhibiting the NBT photochemical reaction by 50% as 1 enzyme activity unit (U).

Data Analysis
The experiment was designed in a completely randomized manner by measuring three independent duplicate datasets, and all data are expressed as means + standard deviation (SD).One-way ANOVA was performed using Duncan's multivariate trial in SPSS Statistical 17 software (SPSS, Chicago, IL, USA).A value of p < 0.05 was considered to be statistically significant.

Weight Loss
The weight loss of the control and coated samples during storage were determined (Figure 1).During storage at room temperature, the weight loss rate of each sample gradually increased and reached the maximum at 100 days of storage (Figure 1).This could be due to a water vapor pressure gradient in different compartments of the fruit cell tissue, through which moisture was transferred from the fruit into the immediate environment [32].Compared with the controls, the weight loss rate of Coatings 2018, 8, 372 5 of 13 the coated samples slowed down at the late storage stage.At 100 days of storage, the weight loss rate of the coated samples was significantly lower than that of the control fruit (p < 0.05), and they exhibited weight reductions of up to 12.46-33.71%.These results are in agreement with previous studies, which reported that weight loss in navel orange coated with 1.5% chitosan was only 46.52% of the control [33].This behavior can be explained by the fact that chitosan forms a film on the surface of the fruit, which hinders the exchange of water vapor, thereby reducing water loss through transpiration [34].

Weight Loss
The weight loss of the control and coated samples during storage were determined (Figure 1).During storage at room temperature, the weight loss rate of each sample gradually increased and reached the maximum at 100 days of storage (Figure 1).This could be due to a water vapor pressure gradient in different compartments of the fruit cell tissue, through which moisture was transferred from the fruit into the immediate environment [32].Compared with the controls, the weight loss rate of the coated samples slowed down at the late storage stage.At 100 days of storage, the weight loss rate of the coated samples was significantly lower than that of the control fruit (p < 0.05), and they exhibited weight reductions of up to 12.46-33.71%.These results are in agreement with previous studies, which reported that weight loss in navel orange coated with 1.5% chitosan was only 46.52% of the control [33].This behavior can be explained by the fact that chitosan forms a film on the surface of the fruit, which hinders the exchange of water vapor, thereby reducing water loss through transpiration [34].

Decay Rate
During storage at room temperature, the decay rate of citrus fruit gradually increased with storage time.The control fruits showed rot on the 20th day of storage, and the decay rate reached 60.67% on day 100 of storage.The main postharvest diseases of citrus fruit decay were blue mold, green mold, and sour rot, which were caused by the infection of Penicillium italicum, Penicillium digitatum, and Oospora citriaurantii, respectively.Compared with the control, the single chitosan coating (CH) significantly reduced the fruit decay rate at the end of storage (Figure 2), mainly because of the antifungal properties of chitosan.Due to its antimicrobial properties, chitosan has been widely used in antimicrobial films to provide edible protective coatings for foods by dipping and spraying [10].Citrus fruits treated with postharvest chitosan had increased resistance to anthracnose, and the incidence of lesions was directly reduced [35].The fruit was treated with 0.5% and 1.0% cinnamaldehyde in the chitosan coating, and the rot rate was significantly reduced (p < 0.05).At day 100 of storage, the fruit decay rates were only 30.10% and 22.82% of the control decay rate, which was significantly lower than that of the chitosan coating (p < 0.05).Cinnamaldehyde showed the ability to control postharvest pathogens of citrus fruit [15].Chitosan reacts with cinnamaldehyde, forming

Decay Rate
During storage at room temperature, the decay rate of citrus fruit gradually increased with storage time.The control fruits showed rot on the 20th day of storage, and the decay rate reached 60.67% on day 100 of storage.The main postharvest diseases of citrus fruit decay were blue mold, green mold, and sour rot, which were caused by the infection of Penicillium italicum, Penicillium digitatum, and Oospora citriaurantii, respectively.Compared with the control, the single chitosan coating (CH) significantly reduced the fruit decay rate at the end of storage (Figure 2), mainly because of the antifungal properties of chitosan.Due to its antimicrobial properties, chitosan has been widely used in antimicrobial films to provide edible protective coatings for foods by dipping and spraying [10].Citrus fruits treated with postharvest chitosan had increased resistance to anthracnose, and the incidence of lesions was directly reduced [35].The fruit was treated with 0.5% and 1.0% cinnamaldehyde in the chitosan coating, and the rot rate was significantly reduced (p < 0.05).At day 100 of storage, the fruit decay rates were only 30.10% and 22.82% of the control decay rate, which was significantly lower than that of the chitosan coating (p < 0.05).Cinnamaldehyde showed the ability to control postharvest pathogens of citrus fruit [15].Chitosan reacts with cinnamaldehyde, forming imine linkages; moreover, cinnamaldehyde crosslinks the chitosan chains [36,37], so the antifungal activity of the composite film was increased, the disease resistance of the fruit was increased, and the fruit rot incidence rate was reduced [20].The decay rate of fruits treated with 1.5% CI-CH was significantly lower than that of the control but there was no significant difference compared with chitosan-treated fruits (p < 0.05).This may be due to the excessive concentration of cinnamaldehyde, increased cracks and roughness on the surface of the composite film, decreased toughness, and the occurrence of voids [38], which may have a certain effect on fruit rot.
Coatings 2018, 8, 372 6 of 13 activity of the composite film was increased, the disease resistance of the fruit was increased, and the fruit rot incidence rate was reduced [20].The decay rate of fruits treated with 1.5% CI-CH was significantly lower than that of the control but there was no significant difference compared with chitosan-treated fruits (p < 0.05).This may be due to the excessive concentration of cinnamaldehyde, increased cracks and roughness on the surface of the composite film, decreased toughness, and the occurrence of voids [38], which may have a certain effect on fruit rot.

Total Soluble Solids, Titratable Acidity, and Vitamin C
The effects of postharvest treatment on the main TSS, TA, and Vc contents of Ponkan fruit were different during room-temperature storage (Figure 3).The TSS content increased initially but later decreased (Figure 3a).The TSS content of the control fruit reached the highest value (12.47%) on day 40 and then decreased.The TSS content at the end of storage was only 11.00%.Coating treatment prolonged the appearance of TSS peaks and delayed the decline of TSS content in the later storage period.At the end of storage, the TSS content of all coated fruits was significantly higher than that of the control.This could be explained by the coating changing the composition of the gas within the fruit (oxygen and carbon dioxide), thereby affecting the respiratory metabolism and delaying the formation and degradation of carbohydrates in the fruit [39].
During storage at room temperature, the TA content of Ponkan fruit gradually decreased (Figure 3b), which was consistent with the changes observed on other citrus fruit, such as Newhall navel orange [40] and Nanfeng mandarin [41].Their organic acids and TA content were observed to decrease during storage.Compared with the control, CH and CI-CH effectively delayed the decrease of TA content in Ponkan fruit, so that the TA contents in coated samples were significantly higher than that in the control at the end of storage.This may be because the coating inhibited the enzyme activity related to organic acid metabolism in citrus fruit, resulting in a decrease in organic acid content.Chitosan coating inhibited the expression of genes that regulate malate metabolism enzyme activity, and delayed the degradation of acid in pear fruit during the storage period [42].
The Vc content of Ponkan fruit gradually increased in the early storage period, and decreased in the middle and late storage periods (Figure 3c).Each coating treatment delayed the degradation of Vc content of the fruit.On the 100th day of storage, the Vc content of the control group was 26.01 mg•kg −1 , and of the fruit treated with CH and 0.5%, 1.0%, and 1.5% CI-CH was 8.66%, 13.85%, 14.72%, and 3.46%, respectively, higher than that of the control.Except for 1.5% CI-CH, the Vc

Total Soluble Solids, Titratable Acidity, and Vitamin C
The effects of postharvest treatment on the main TSS, TA, and Vc contents of Ponkan fruit were different during room-temperature storage (Figure 3).The TSS content increased initially but later decreased (Figure 3a).The TSS content of the control fruit reached the highest value (12.47%) on day 40 and then decreased.The TSS content at the end of storage was only 11.00%.Coating treatment prolonged the appearance of TSS peaks and delayed the decline of TSS content in the later storage period.At the end of storage, the TSS content of all coated fruits was significantly higher than that of the control.This could be explained by the coating changing the composition of the gas within the fruit (oxygen and carbon dioxide), thereby affecting the respiratory metabolism and delaying the formation and degradation of carbohydrates in the fruit [39].
During storage at room temperature, the TA content of Ponkan fruit gradually decreased (Figure 3b), which was consistent with the changes observed on other citrus fruit, such as Newhall navel orange [40] and Nanfeng mandarin [41].Their organic acids and TA content were observed to decrease during storage.Compared with the control, CH and CI-CH effectively delayed the decrease of TA content in Ponkan fruit, so that the TA contents in coated samples were significantly higher than that in the control at the end of storage.This may be because the coating inhibited the enzyme activity related to organic acid metabolism in citrus fruit, resulting in a decrease in organic acid content.Chitosan coating inhibited the expression of genes that regulate malate metabolism enzyme activity, and delayed the degradation of acid in pear fruit during the storage period [42].
The Vc content of Ponkan fruit gradually increased in the early storage period, and decreased in the middle and late storage periods (Figure 3c).Each coating treatment delayed the degradation of Vc content of the fruit.On the 100th day of storage, the Vc content of the control group was 26.01 mg•kg −1 , and of the fruit treated with CH and 0.5%, 1.0%, and 1.5% CI-CH was 8.66%, 13.85%, 14.72%, and 3.46%, respectively, higher than that of the control.Except for 1.5% CI-CH, the Vc content of other coated fruits was significantly higher than that of the control (p < 0.05), which was consistent with the changes observed in navel orange [33].
These results indicate that cinnamaldehyde-chitosan coating helped to delay the decline of nutritive quality of Ponkan fruit.content of other coated fruits was significantly higher than that of the control (p < 0.05), which was consistent with the changes observed in navel orange [33].
These results indicate that cinnamaldehyde-chitosan coating helped to delay the decline of nutritive quality of Ponkan fruit.

Color Index
Changes of the color index (CCI) of Ponkan fruit peel during room-temperature storage were determined (Figure 4).Ponkan is a late-ripening citrus variety.Its peel coloration time is late and the degree of coloration is relatively weak.In order to avoid low-temperature damage, the fruits were harvested when the pulp had reached maturity and the skin was not partially colored [29].When storage began, the fruit's CCI value was lower than 1 (Figure 4).With longer storage time, the color of the peel turned from green to pale yellow, and the CCI values gradually increased.This is due to the decrease in chlorophyll content in the citrus peel and the increase in carotenoid content during storage at room temperature [43].There was no significant difference between the color difference of the control fruit and CH and 0.5% or 1.0% CI-CH fruit.However, the CCI value of the 1.5% CI-CH fruit was significantly lower than that of the control during storage (p < 0.05).This indicated that the chitosan coating and the low concentration of cinnamaldehyde in the coating did not affect the color change of the citrus peel after harvest.However, a high concentration of cinnamaldehyde-chitosan coating (1.5% CI-CH) did affect the normal color change of the citrus peel, and made the CCI value of 1.5% CI-CH fruit significantly lower than that of the control at the end of storage (p < 0.05).This may be due to the phytotoxic effect of an excessive concentration of cinnamaldehyde in 1.5% CI-CH [44].

Color Index
Changes of the color index (CCI) of Ponkan fruit peel during room-temperature storage were determined (Figure 4).
Ponkan is a late-ripening citrus variety.Its peel coloration time is late and the degree of coloration is relatively weak.In order to avoid low-temperature damage, the fruits were harvested when the pulp had reached maturity and the skin was not partially colored [29].When storage began, the fruit's CCI value was lower than 1 (Figure 4).With longer storage time, the color of the peel turned from green to pale yellow, and the CCI values gradually increased.This is due to the decrease in chlorophyll content in the citrus peel and the increase in carotenoid content during storage at room temperature [43].There was no significant difference between the color difference of the control fruit and CH and 0.5% or 1.0% CI-CH fruit.However, the CCI value of the 1.5% CI-CH fruit was significantly lower than that of the control during storage (p < 0.05).This indicated that the chitosan coating and the low concentration of cinnamaldehyde in the coating did not affect the color change of the citrus peel after harvest.However, a high concentration of cinnamaldehyde-chitosan coating (1.5% CI-CH) did affect the normal color change of the citrus peel, and made the CCI value of 1.5% CI-CH fruit significantly lower than that of the control at the end of storage (p < 0.05).This may be due to the phytotoxic effect of an excessive concentration of cinnamaldehyde in 1.5% CI-CH [44].

Malondialdehyde
During storage at room temperature, the MDA content of Ponkan fruit gradually increased (Figure 5).This was because the fruit gradually aged as the storage time went on, and the degree of membrane lipid oxidation of the fruit increased.The MDA contents of CH, 0.5% CI-CH, and 1.0% CI-CH were significantly lower than that of the control fruit (p < 0.05).On day 100 of storage, the MDA contents of CH and 0.5% and 1.0% CI-CH fruits were 79.71%, 62.00%, and 58.60%, respectively, of that of the control fruits (3.00 mmol•g -1 ).This was because the chitosan and low-concentration cinnamaldehyde coating delayed the senescence of Ponkan fruit during postharvest storage, reduced membrane lipid peroxidation, and hence reduced the MDA content.Hong and colleagues [39] also showed that treatment with 2.0% chitosan coating delayed the changes of MDA content in Pearl guava fruit during cold storage and induced a significant increase in resistant enzyme activity.However, the MDA content of 1.5% CI-CH fruits increased sharply in the early storage period, and was significantly higher than that of other treated fruits in the late storage period (Figure 5).This could be due to the higher concentration of cinnamaldehyde in the coating treatment, which can

Malondialdehyde
During storage at room temperature, the MDA content of Ponkan fruit gradually increased (Figure 5).This was because the fruit gradually aged as the storage time went on, and the degree of membrane lipid oxidation of the fruit increased.The MDA contents of CH, 0.5% CI-CH, and 1.0% CI-CH were significantly lower than that of the control fruit (p < 0.05).On day 100 of storage, the MDA contents of CH and 0.5% and 1.0% CI-CH fruits were 79.71%, 62.00%, and 58.60%, respectively, of that of the control fruits (3.00 mmol•g -1 ).This was because the chitosan and low-concentration cinnamaldehyde coating delayed the senescence of Ponkan fruit during postharvest storage, reduced membrane lipid peroxidation, and hence reduced the MDA content.Hong and colleagues [39] also showed that treatment with 2.0% chitosan coating delayed the changes of MDA content in Pearl guava fruit during cold storage and induced a significant increase in resistant enzyme activity.However, the MDA content of 1.5% CI-CH fruits increased sharply in the early storage period, and was significantly higher than that of other treated fruits in the late storage period (Figure 5).This could be due to the higher concentration of cinnamaldehyde in the coating treatment, which can damage the epidermal cells of the fruit, increase the permeability of the cell membranes, and rapidly accumulate MDA content [45].damage the epidermal cells of the fruit, increase the permeability of the cell membranes, and rapidly accumulate MDA content [45].

Activities of Catalase, Peroxidase, and Superoxide Dismutase
In response to stress, plants normally increase the activity of catalase, peroxidase, and superoxide dismutase.Moreover, a decrease in enzymatic potential may be associated with a reduction in the capacity to prevent damage [26].
The change of CAT activity of Ponkan fruits during storage at room temperature was determined (Figure 6a), and the activity of the control fruit, which decreased slowly in the early storage period and reached the maximum (37.6%) at 60 days of storage, was higher than the initial value (0.27 U/g min).Compared with the control fruit, the CAT activity of CH-treated fruits decreased significantly in the late storage period and was significantly higher than its counterpart control at 100 days of storage (p < 0.05).This was similar in the case of the study of the effect of calcium chloride, chitosan, and pullulan coatings on the antioxidant activity of Huangguan pears during storage.Chitosan coating treatment slowed the accumulation of total phenolic and flavonoid content, chlorogenic acid, arbutin, catechin, and caffeic acid, and reduced SOD and CAT activity as well as total antioxidant activity in pear fruits during storage [46].The CAT activity in 0.5% and 1.0% CI-CH fruit significantly increased in the early storage period, and remained at a higher level during subsequent storage.The CAT activity of 0.5% and 1.0% CI-CH was significantly higher compared to that of the control and CH fruits at the end of storage (p < 0.05).This was due to the low concentration of cinnamaldehyde, which increased the antioxidant enzyme activity in the fruits during storage [15].
The changes of POD and SOD activities of Ponkan fruit were basically the same during storage at room temperature-they increased initially and later decreased (Figure 6b,c).The POD and SOD activities of control, CH, and 1.5% CI-CH fruits peaked at day 40 of storage.The 0.5% and 1.0% CI-CH treatments were able to delay the highest peaks of POD and SOD activities, which peaked at 60 days of storage.The maximum values of POD and SOD activities of CH and 0.5% and 1.0% CI-CH fruits were significantly higher than those of the control (p < 0.05).The coating treatment significantly delayed the decrease of POD and SOD activities in Ponkan fruit during storage, which made the POD and SOD activities of CH and 0.5% and 1.0% CI-CH fruits significantly higher than those of the control (p < 0.05).On day 100 of storage, the POD and SOD activities of the control fruits were 48.05 u/g•min and 13.39 u/g FW, respectively.In the fruits treated with CH and 0.5% and 1.0% CI-CH, the POD activity was 3.57, 4.83, and 4.47 times that of the control, while the SOD activity was 1.65, 2.54,

Activities of Catalase, Peroxidase, and Superoxide Dismutase
In response to stress, plants normally increase the activity of catalase, peroxidase, and superoxide dismutase.Moreover, a decrease in enzymatic potential may be associated with a reduction in the capacity to prevent damage [26].
The change of CAT activity of Ponkan fruits during storage at room temperature was determined (Figure 6a), and the activity of the control fruit, which decreased slowly in the early storage period and reached the maximum (37.6%) at 60 days of storage, was higher than the initial value (0.27 U/g min).Compared with the control fruit, the CAT activity of CH-treated fruits decreased significantly in the late storage period and was significantly higher than its counterpart control at 100 days of storage (p < 0.05).This was similar in the case of the study of the effect of calcium chloride, chitosan, and pullulan coatings on the antioxidant activity of Huangguan pears during storage.Chitosan coating treatment slowed the accumulation of total phenolic and flavonoid content, chlorogenic acid, arbutin, catechin, and caffeic acid, and reduced SOD and CAT activity as well as total antioxidant activity in pear fruits during storage [46].The CAT activity in 0.5% and 1.0% CI-CH fruit significantly increased in the early storage period, and remained at a higher level during subsequent storage.The CAT activity of 0.5% and 1.0% CI-CH was significantly higher compared to that of the control and CH fruits at the end of storage (p < 0.05).This was due to the low concentration of cinnamaldehyde, which increased the antioxidant enzyme activity in the fruits during storage [15].
The changes of POD and SOD activities of Ponkan fruit were basically the same during storage at room temperature-they increased initially and later decreased (Figure 6b,c).The POD and SOD activities of control, CH, and 1.5% CI-CH fruits peaked at day 40 of storage.The 0.5% and 1.0% CI-CH treatments were able to delay the highest peaks of POD and SOD activities, which peaked at 60 days of storage.The maximum values of POD and SOD activities of CH and 0.5% and 1.0% CI-CH fruits were significantly higher than those of the control (p < 0.05).The coating treatment significantly delayed the decrease of POD and SOD activities in Ponkan fruit during storage, which made the POD and SOD activities of CH and 0.5% and 1.0% CI-CH fruits significantly higher than those of the control (p < 0.05).On day 100 of storage, the POD and SOD activities of the control fruits were 48.05 u/g•min and 13.39 u/g FW, respectively.In the fruits treated with CH and 0.5% and 1.0% CI-CH, the POD activity was 3.57, 4.83, and 4.47 times that of the control, while the SOD activity was 1.65, 2.54, and 2.74 times that of the control, respectively.The chitosan coating lessened the aging of fruit during storage, and the antiaging ability of the fruit was enhanced by adding the antifungal component cinnamaldehyde to the chitosan coating.Consistent with Xing and co-workers [23], we found that the chitosan-cinnamaldehyde coating could maintain a high level of antioxidant activity of enzymes such as SOD and POD in sweet pepper preserved for 35 days.
Coatings 2018, 8, x FOR PEER REVIEW 10 of 13 and 2.74 times that of the control, respectively.The chitosan coating lessened the aging of fruit during storage, and the antiaging ability of the fruit was enhanced by adding the antifungal component cinnamaldehyde to the chitosan coating.Consistent with Xing and co-workers [23], we found that the chitosan-cinnamaldehyde coating could maintain a high level of antioxidant activity of enzymes such as SOD and POD in sweet pepper preserved for 35 days.

2. 4 .
Quality Determinations 2.4.1.Weight Loss Weight loss was determined with samples of 20 fruits per treatment.The fruits from each treatment were weighed at 0, 20, 40, 60, 80, and 100 days of storage.The weight loss was calculated as follows: weight loss = initial weight − weighing weight initial weight × 100%

Figure 1 .
Figure 1.Effect of cinnamaldehyde composite coating on weight loss of mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.

Figure 1 .
Figure 1.Effect of cinnamaldehyde composite coating on weight loss of mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.

Figure 2 .
Figure 2. Effect of cinnamaldehyde composite coating on the decay rate of mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.

Figure 2 .
Figure 2. Effect of cinnamaldehyde composite coating on the decay rate of mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.

Figure 3 .Figure 3 .
Figure 3.Effect of cinnamaldehyde composite coating on (a) total soluble solids (TSS), (b) titratable ability (TA), and (c) vitamin C (Vc) of mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with different lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.

Figure 4 .
Figure 4. Effect of cinnamaldehyde composite coating on the color composition index (CCI) of mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with different lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.

Figure 4 .
Figure 4. Effect of cinnamaldehyde composite coating on the color composition index (CCI) of mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with different lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.

Figure 5 .
Figure 5.Effect of cinnamaldehyde composite coating on the malondialdehyde (MDA) content of mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with different lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.

Figure 5 .
Figure 5.Effect of cinnamaldehyde composite coating on the malondialdehyde (MDA) content of mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with different lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.

Figure 6 .Figure 6 .
Figure 6.Effect of cinnamaldehyde composite coating on activities of (a) CAT, (b) POD, and (c) SOD in mandarin fruits cv.Ponkan during room-temperature storage.Each value represents the mean ± SD of three replicates; those marked with different lowercase letters are significantly different at p ≤ 0.05 according to Duncan's multiple range test.