Improving the Shelf Life of Peeled Fresh Almond Kernels by Edible Coating with Mastic Gum

: Coating, as a process in which fruits, vegetables, kernels, and nuts are covered with an edible layer, is an environmentally friendly alternative to plastic wrapping, which has been considered the most effective way to preserve them over the long term. On the other hand, prolonging the shelf life results in a reduction of spoilage and therefore achieving a goal that is very important nowadays— the reduction of food waste. The quality of preserved almonds kernels depends on factors such as grain moisture, storage temperature, relative humidity, oxygen level, packaging, and the shape of the stored nuts (along with being peeled, unpeeled, roasted, etc.). The commercial importance of the almond fruit is related to its kernel. Almonds that are peeled (without the thin brown skin) and stored have a shorter shelf life than unpeeled almonds since the reddish-brown skin, rich in antioxidants, may protect the kernels against oxidation. In this study, a bioactive edible coating has been tested, which may provide an effective barrier against oxygen permeation and moisture, thus preserving the quality of peeled fresh almonds by extending their shelf life. Mastic gum, as a natural coating agent, was used to coat the peeled fresh almond kernels in four different concentrations (0.5%, 1.0%, 1.5%, and 2.0% w / v ). The effect of mastic gum coating on the quality parameters of the peeled fresh almonds (moisture uptake, oil oxidation, total yeast and mold growth, and Aspergillus species development) was studied during four months of storage. The results showed that mastic gum, as a coating agent, signiﬁcantly ( p < 0.05) reduced moisture absorption, peroxide and thiobarbituric acid indices, total yeast and mold growth, and Aspergillus species development in the peeled and coated fresh almonds, compared to the control, i.e., uncoated fresh almonds, during 4 months of storage, packed at room temperature (25–27 ◦ C) inside a cabinet at 90% humidity. Therefore, mastic gum can be used as a great natural preservative coating candidate with antioxidant and antimicrobial effects.


Introduction
Currently, researchers are testing innovative protective layers that are supposed to keep the fruit, vegetables, and kernels fresher longer. The layers, also called edible coating, are colorless, odorless, tasteless, and harmless to health, and they can even be consumed without hesitation since they consist of natural or microbiological sources. The edible coating can reduce packaging waste, food waste, and greenhouse gases. It is sprayed or spread on fruit, vegetables, and kernels and creates a barrier that controls how much water Moreover, almonds that are peeled (skinless, without the thin brown skin) and stored have a shorter shelf life than unpeeled almonds since the reddish-brown skin, rich in antioxidants, may have a protective role against oxidation, but many food recipes call for skinless almonds [25,30]. Packaging and storing conditions are very important factors to prevent oxidation and maintain optimum flavor, avoiding, at the same time, concealed damage.
In order to extend the shelf life of nuts, including almonds, and retard lipid oxidation, research has been carried out extensively to study the use of bio-based edible coatings. The edible coating may provide an effective barrier against oxygen permeation and moisture, thus preserving the quality of nuts by extending their shelf life [31][32][33][34].
Due to the increasing demand for sources of bioactive biodegradable/edible films and coatings, there is a need to explore the novel and underutilized sources of raw materials with functional properties for preservation and shelf-life extension of foods [35]. Therefore, natural tree resins, excreted by trees or bushes when injured, and natural tree gums, may be promising alternatives for coating, as substitutes for materials from nonrenewable sources [36].
Mastic gum, a plant resin, is obtained from a special type of pistachio, a variety of Pistacia lentiscus (family: Anacardiaceae). The viscous resin emerges in tear shaped droplets from the bark of the mastic bush and therefore also called "tears".
Several studies on the biological properties of mastic gum have shown positive therapeutic effects in anticancer activity, improving the immune system, cardiovascular disease, inhibiting free radicals, inhibiting lipid peroxidation, and stimulating the activity of antioxidant enzymes. Ancient doctors recommended this sap to treat periodontal disease or pimples. The sap was also used for digestive problems, such as stomach pain, heartburn, and stomach ulcers, as well as for wound treatment or rheumatic complaints. It has been shown that mastic acts throughout the gastrointestinal system or that it lowers cholesterol, blood sugar, and blood pressure. New studies confirm its antibacterial effectiveness: germs that are responsible for the formation of caries and periodontitis can be successfully reduced with mastic. There are also studies on the effects on oral hygiene, for example, in the case of tooth decay, xerostomia (dry mouth or oral dryness), or gingivitis, and on the skin [37][38][39][40][41].
Due to the numerous positively proven bioactive properties, mastic gum received increasing attention, and the demand for it has increased in recent years. Moreover, in different areas such as food supplements, general cosmetic products, toothpaste or mouthwash products, industrial uses, animal nutrition, and for natural flavors in food, confectionery, and beverages, there is a need for bio-based materials, such as natural materials, as substitutes for materials from nonrenewable sources replacing traditional petroleum polymers in several industrial sectors, which provide additional properties, such as antimicrobial, antioxidant, anti-inflammatory effects. Almond kernels during storage are highly exposed to fungi contamination, resulting in the production of toxins (metabolites produced by Aspergillus species) and increased mold and yeast counts. However, most of the cases, contaminated almonds leave no visually visible wounds or discoloration, posing a risk to public health can lead in the seemingly defect-free final product [42].
A low temperature (around 4 • C), as an ideal storage parameter, seems to reduce aflatoxin levels and mold and yeast count for 3-6 months [43].
The aim of this study was to investigate the effect of bioactive edible coating of mastic gum on fresh almond kernels (variety Carmel cultivar from Artin Azma, Lorestan Province, Iran), and to evaluate the quality parameters (moisture uptake, oil oxidation, total yeast, and mold growth, and Aspergillus species development) during 4 months of the storage period, packed at room temperature (25-27 • C) inside a cabinet and at 90% humidity.

Materials
To conduct the present research, mastic gum (Pistacia lentiscus) was purchased from the medicinal plants' store city (Artin Azma, Lorestan Province, Iran), and the fresh almond from cultivar Carmel was purchased from Lorestan Province, Iran. All chemicals needed and plate count agar (PCA) medium were purchased from Merck (Hamburg, Germany).

Edible Coating Preparation
In order to prepare mastic gum solutions with concentrations of 0.5%, 1%, 1.5%, and 2% (w/v), respectively, 5, 10, 15, and 20 g of mastic gum powder were added to one liter of distilled water, slowly and in several steps. The solution was then stirred with a glass stirrer. The solution was filtered afterward with Whatman grade 3 qualitative filter paper Ø 185 mm.

Covering, Packing, and Storage Study of Fresh Almond Kernels
First, after raw almonds were dehulled (separating the green skins), the whole fresh almond seeds were dried using a cabinet dryer (Artin Azma, Yavari st, Madani Ave, Resalat.Ave Tehran, Iran) at 43 • C until a moisture content of 5% (AOAC, 2005) was achieved [44]. Then, the almonds were crushed manually, to remove the shell, and peeled (skinless, without the thin brown skin), and healthy kernels were selected. The hull, shell, and skin are considered almond by-products [45]. The peeled fresh almond kernels were then weighed and placed in lattice containers.
Afterward, they were immersed in containers with the corresponding coating solution for 30 s and removed. After coating, to remove excess moisture, the almonds were left for the excess solution to drop off, and to dry off the excess moisture, they were dried in an oven at 40 • C for 4 h until they reached an average humidity of 2.5% [46,47].
Almonds were completely randomly divided into 50 g units using a digital scale (A&D Co, LTD, Lorestan, Japan) with an accuracy of 0/001 and packed in commercial plastic bags with dimensions of 6 cm × 20 cm. Packed almonds were stored for 4 months at room temperature (25-27 • C) inside a cabinet and at 90% humidity.
The percentage of moisture was calculated according to the following equation: where, m w = mass of the water; m sample = mass of the sample.
Assessment of Oxidation Measurement of Peroxide Index (PI) Almond kernel oil was extracted under room temperature (25-27 • C) condition in a dark place using normal hexane solvent [50], and peroxide index, as an indicator of primary oxidation product, was measured by an iodometric method in accordance with the standard of the American Oil Chemists' Society (AOCS, 2003) [51].

Total Yeast and Mold Counts (TYMC)
To perform the total yeast and mold counts, 10 g of each uncoated and coated almonds were pounded with a sterile mortar under the flame and the microbial hood (Karen Pouya Noavar, Tehran, North Kargar St Boulevard Saman, Tower Iran). Almonds powder, along with 90 mL of 0.9% sterilized sodium chloride solution, was transferred to a stomacher bag and mixed thoroughly in the stomacher device (Stomacher Bag Mixer 400 SW Interscience, Artin Azma, Iran). Dilutions 10 −2 and 10 −3 were obtained from dilutions of 10 −1 . A total of 0.1 mL of each dilution was added to the PCA solid culture medium using a sampler and completely spread on the culture medium using a special glass rod (Arian Company, Artin Azma, Iran).
After a few minutes, the plates were heated upside down at 20 to 25 • C. After 5 days, the number of grown mold and yeast were counted and reported in cfu/gr unit.

Antifungal Characteristic of Mastic Gum against the Growth of Aspergillus Species
For this purpose, 10 g of almond kernels from each of the coated and uncoated samples were placed in large glass plates containing wet filter paper and all of which were sterilized and then incubated at 25 • C. After 5 days, the number of plates infected with fungi was counted, and the rate of fungi development was determined based on the percentage in each sample (coated almond kernels), compared to the control sample (uncoated almond kernels).
If necessary, the fungi grown on almond kernels were cultured on a culture slide, and the growth of Aspergillus species was ensured under an electronic microscope.
All measurements were measured at intervals of 1 month to 4 months in three replications.

Statistical Analysis
The experiments were performed in three replications in a completely randomized design, and the obtained data were statistically analyzed. After analysis of variance, the means were compared using Duncan's multiple range test at the level of 0.05 by SAS (Statistical Analysis System) software (version 9.2). Word Excel 2013 software was used to draw the graphs.

Fresh Skinless Almond Kernels' Nutritional Chemical Composition
The nutritional composition of fresh almond kernels used in the current study, before the coating, is shown in Table 1. Almond kernels composition is of great interest from the nutritional points of view and therefore very important for commercial and industrial applications. It seems that the almond kernels used in this study present compounds in concentrations reported already with high nutritional value [14][15][16][17][18][19]. According to Table 1, the fresh almond kernels are very fatty, with-62.0% ± 01.06% of fat, and if their storage conditions are unsuitable, this aspect makes them susceptible to lipid oxidation, rancidity development, and concealed damage. This may influence shelf life, resulting in a very bitter off-flavor and a low acceptance rate among consumers [9][10][11][22][23][24][25][26][27][28][29][30].

On Moisture Content
Humidity is one of the important factors in maintaining the quality of kernels. The rate of moisture transfer between food and the surrounding atmosphere is reduced by completely covering the food with film or by coating [54]. Figure 1 shows the changes in the moisture content (%) of fresh almond kernels uncoated and coated with different mastic gum concentrations over a 4-month storage period conditions. As can be observed, although the average amount of moisture absorption in all samples during the storage period is associated with an increasing trend, the coating with mastic gum significantly reduced moisture absorption, compared to uncoated samples, during the storage period.

On Moisture Content
Humidity is one of the important factors in maintaining the quality of kernels. The rate of moisture transfer between food and the surrounding atmosphere is reduced by completely covering the food with film or by coating [54]. Figure 1 shows the changes in the moisture content (%) of fresh almond kernels uncoated and coated with different mastic gum concentrations over a 4-month storage period conditions. As can be observed, although the average amount of moisture absorption in all samples during the storage period is associated with an increasing trend, the coating with mastic gum significantly reduced moisture absorption, compared to uncoated samples, during the storage period.

On Peroxide Index
Peroxide index (PI) is the most common sign of oxidative spoilage in kernels [55]. Oxidation of lipids in food is one of the most important factors in food degradation during processing, storage, and distribution and leads to adverse effects on aroma, color, nutritional value, and the production of toxic compounds [56].
The presence of unsaturated fatty acids makes almond oil susceptible to oxidation, as previously mentioned. The effect of coating with mastic gum in different concentrations on the trend of changes in almond kernel peroxide index during four months of storage period and conditions, based on mill equivalents of oxygen per kg of almond oil, is presented in Table 2. The results show that the trend of changes in the peroxide index Figure 1. Changes in the moisture content (%) of uncoated (control) and coated almond kernels during 4 months of storage. Different lowercase letters indicate a statistically significant differences (p < 0.05) between the samples during each month; share a lowercase letter are not statistically different, while different letters denote for statistical differences at least 95% confidence.

On Peroxide Index
Peroxide index (PI) is the most common sign of oxidative spoilage in kernels [55]. Oxidation of lipids in food is one of the most important factors in food degradation during processing, storage, and distribution and leads to adverse effects on aroma, color, nutritional value, and the production of toxic compounds [56].
The presence of unsaturated fatty acids makes almond oil susceptible to oxidation, as previously mentioned. The effect of coating with mastic gum in different concentrations on the trend of changes in almond kernel peroxide index during four months of storage period and conditions, based on mill equivalents of oxygen per kg of almond oil, is presented in Table 2. The results show that the trend of changes in the peroxide index of all samples increased significantly during the storage period. The PI of fresh almond kernel uncoated (control) was 0.24 mill equivalents of oxygen per kilogram, and after 4 months under storage conditions, PI reached 1.12 mill equivalents of oxygen per kilogram of oil. The highest and lowest PI values, after 4 months of storage, were related to the control sample (uncoated almonds) and the sample of almonds coated with 2% (w/v) mastic gum, respectively. The PI values of the almond samples coated with 1.5% and 2% (w/v) of mastic gum were not significantly different, as can be seen; concluding that coating with 1.5% mastic gum offers good almonds protection as well.

On Thiobarbituric Acid Index (TBA)
Measurement of TBA index is a method for detecting by-products of fat oxidation in fatty food products [57]. By-products change the taste of the product, cause food spoilage, and have adverse effects on the nutritional value of the product, which makes it difficult to use kernels in other products [58]. The changes in the value of the TBA index in the tested uncoated and coated almonds samples are shown in Table 3. Table 3. Changes in the peroxide value of uncoated (control) and coated almonds during 4 months storage in mill equivalents of oxygen per kilogram of oil. As can be observed in Table 3, the TBA index increased significantly during the storage period in uncoated almonds samples, compared to almonds coated with 1%, 1.5%, and 2% (w/v) mastic gum. There is no significant difference between control and coating with 0.5% mastic gum, thus providing slight protection.

Microbiological Analysis Results Total Yeast and Mold Counts (TYMC)
The results of the TYMC in almond kernels uncoated (control) and coated with different concentrations of mastic gum during the 4-month storage period are shown in Figure 2. According to the results obtained during the storage period, the growth rate of mold and yeast in the control sample increased significantly, while coated almonds with mastic gum significantly reduced the overall mold and yeast, compared to the control sample, at each stage of sampling during the storage period. In the middle of the storage period (day 105), differences in the mean values among treatments began to appear.

Total Yeast and Mold Counts (TYMC)
The results of the TYMC in almond kernels uncoated (control) and coated with different concentrations of mastic gum during the 4-month storage period are shown in Figure 2. According to the results obtained during the storage period, the growth rate of mold and yeast in the control sample increased significantly, while coated almonds with mastic gum significantly reduced the overall mold and yeast, compared to the control sample, at each stage of sampling during the storage period. In the middle of the storage period (day 105), differences in the mean values among treatments began to appear. The total number of mold and yeast increased from 1,150 to 11,700 cfu/gr after 4 months of storage in the control sample, while the total number of mold and yeast in the samples coated with 2% (w/v) mastic gum decreased from 11,700 to 3,700 cfu/gr, compared to the uncoated sample.

Antifungal Characteristic of Mastic Gum Coating against the Growth of Aspergillus Species
Aflatoxins may occur in contaminated almonds, and, in general, in contaminated foods, with fungi from Aspergillus species as carcinogenic secondary metabolites. Factors such as environmental conditions (e.g., temperature, relative humidity) affect mold growth and aflatoxin production [59].
The contamination with Aspergillus spp. of almonds, as in other nuts (such as peanut, hazelnut, walnut), occurs generally during harvest, processing, and storage. It is very important to prevent the postharvest mold contamination and growth of the susceptible almonds, and nuts. [1,42].
The results of the present study regarding Aspergillus spp. mold development on fresh almond kernels uncoated and coated with different mastic gum concentrations during the 4 months of storage period are shown in Figure 3. As can be seen in the figure, mastic gum coating significantly reduced the growth rate of Aspergillus spp. Mold, compared to control samples. The total number of mold and yeast increased from 1150 to 11,700 cfu/gr after 4 months of storage in the control sample, while the total number of mold and yeast in the samples coated with 2% (w/v) mastic gum decreased from 11,700 to 3700 cfu/gr, compared to the uncoated sample.

Antifungal Characteristic of Mastic Gum Coating against the Growth of Aspergillus Species
Aflatoxins may occur in contaminated almonds, and, in general, in contaminated foods, with fungi from Aspergillus species as carcinogenic secondary metabolites. Factors such as environmental conditions (e.g., temperature, relative humidity) affect mold growth and aflatoxin production [59].
The contamination with Aspergillus spp. of almonds, as in other nuts (such as peanut, hazelnut, walnut), occurs generally during harvest, processing, and storage. It is very important to prevent the postharvest mold contamination and growth of the susceptible almonds, and nuts [1,42].
The results of the present study regarding Aspergillus spp. mold development on fresh almond kernels uncoated and coated with different mastic gum concentrations during the 4 months of storage period are shown in Figure 3. As can be seen in the figure, mastic gum coating significantly reduced the growth rate of Aspergillus spp. Mold, compared to control samples.  The contamination with aflatoxins produced by Aspergillus spp. represents a major public health concern and, in addition to the possible contamination with other kinds of mold and yeast, causes food spoilage. Investigations on minimizing or eliminating such contaminants are an ongoing research topic, and the results of the present study contribute with new results to this area of research.

Discussion
Plant-based foods that are consumed raw are susceptible to changes in quality during processing. They can be contaminated with germs and thus represent a source of food infections. Dry foods such as almonds, nuts, dried fruits, spices, milk powder, or dried teas are not excluded. Almond kernels are very fatty, rich in oleic (main monounsaturated fatty acids) and linoleic acids (polyunsaturated fatty acids). The ratio of these two fatty acids is at a higher level than in the various common nut oils, vegetable oils (e.g., soybean), and seed (e.g., pomegranate, grape, date, and sesame) oils [28,60].
Moreover, the high content of these fatty acids in almonds, contributing to shorter shelf life, compared to other nuts, in addition to the presence of riboflavin as a photosensitizer in almonds, makes almonds susceptible to auto-oxidation, which is the main cause of off-flavor development in almonds, both in wild or domestic species of almonds. Environmental factors (e.g., storage temperature, packaging techniques, moisture, and light) play an active role in influencing lipid oxidation [28,61].
Several suitable packaging materials and techniques (e.g., modified atmosphere packaging or vacuum packaging) and storage temperatures have been tested and proposed to extend the shelf-life period of various types of nuts and almond kernel products [28,[62][63][64][65][66]. Major challenges during storage and limitations for usage, for example, as snack mix nuts and almond products, may be considered.
The quality of almonds and other nuts can be affected by moisture, another important factor to be taken into consideration. When moisture is too high, it influences the deteriorative reactions, and therefore, mold growth occurs. However, too low levels of moisture have another kind of influence on the kernel shrivel [67].
Recently the application of edible coating based on bio-based antimicrobial and bioactive materials, such as natural plant materials or from different renewable resources, Figure 3. Percentage of the growth of Aspergillus species on almond uncoated (control) and coated with different concentrations of mastic gum during 4 months of storage. Different lowercase letters indicate a statistically significant differences (p < 0.05) between the samples during each month; share a lowercase letter are not statistically different, while different letters denote for statistical differences at least 95% confidence.
The contamination with aflatoxins produced by Aspergillus spp. represents a major public health concern and, in addition to the possible contamination with other kinds of mold and yeast, causes food spoilage. Investigations on minimizing or eliminating such contaminants are an ongoing research topic, and the results of the present study contribute with new results to this area of research.

Discussion
Plant-based foods that are consumed raw are susceptible to changes in quality during processing. They can be contaminated with germs and thus represent a source of food infections. Dry foods such as almonds, nuts, dried fruits, spices, milk powder, or dried teas are not excluded. Almond kernels are very fatty, rich in oleic (main monounsaturated fatty acids) and linoleic acids (polyunsaturated fatty acids). The ratio of these two fatty acids is at a higher level than in the various common nut oils, vegetable oils (e.g., soybean), and seed (e.g., pomegranate, grape, date, and sesame) oils [28,60].
Moreover, the high content of these fatty acids in almonds, contributing to shorter shelf life, compared to other nuts, in addition to the presence of riboflavin as a photosensitizer in almonds, makes almonds susceptible to auto-oxidation, which is the main cause of off-flavor development in almonds, both in wild or domestic species of almonds. Environmental factors (e.g., storage temperature, packaging techniques, moisture, and light) play an active role in influencing lipid oxidation [28,61].
Several suitable packaging materials and techniques (e.g., modified atmosphere packaging or vacuum packaging) and storage temperatures have been tested and proposed to extend the shelf-life period of various types of nuts and almond kernel products [28,[62][63][64][65][66]. Major challenges during storage and limitations for usage, for example, as snack mix nuts and almond products, may be considered.
The quality of almonds and other nuts can be affected by moisture, another important factor to be taken into consideration. When moisture is too high, it influences the deterio-rative reactions, and therefore, mold growth occurs. However, too low levels of moisture have another kind of influence on the kernel shrivel [67].
Recently the application of edible coating based on bio-based antimicrobial and bioactive materials, such as natural plant materials or from different renewable resources, as substitutes for materials from nonrenewable sources, has received attention, contributing to extending the shelf life of natural perishable products while developing healthy and natural foods. The use of edible films (e.g., polysaccharides and proteins) for extending the shelf life of different nuts, including almonds, has been studied, and research is still ongoing [1,[31][32][33][34]52,[68][69][70].
The main objective of the present study was to enhance the durability of stored fresh almond kernels without becoming spoiled by pathogens and affected by moisture, which are the routes that cause food spoilage. Almond kernels, as the main commercial importance of almond fruit, are widely used in different food products; lately, snack mix nuts and almonds are the top products preferred. Even athletes appreciate almond kernels for their plant-based proteins and their essential amino acids. Moreover, recent studies have explored the effect of other nutritional compounds of almonds such as fiber on gut microbiota or the antioxidant capacity of the protein fraction [71][72][73][74][75].
In the present study, mastic gum was analyzed to be used as an alternative for fresh almonds coating for preservation, against contamination with molds, yeast, and fungi during storage over 4 months. Many countries determined the toxin limits for some foods such as pistachio, almonds, peanut, walnut, and hazelnut. Additionally, most importantly is that EU imports of different Iranian nuts decreased due to the increasing contamination risk incidents originated from Iran [76,77]. If contaminated almonds enter the production facilities after harvest, they can also contaminate other batches.
Evaluation of the sample's moisture content during the storage period showed a statistically significant difference (p < 0.05) between the samples with mastic gum coating and the control sample. By increasing the concentration of mastic gum, the moisture content of almond kernels during the storage period was decreased.
These results indicate that the mastic gum coating, as a barrier, reduces the transfer of moisture to the almond kernel tissue and increases its inhibitory effect by increasing the thickness of the coating. Similar results were achieved on the effect of coating almonds or other nuts kernels with different kinds of edible coating materials, and with increasing the thickness of the coating, its inhibitory effect increases [68][69][70][78][79][80][81].
Moreover, the oxidation potential is increased, and the fats have a longer shelf life, i.e., the almonds do not go rancid as quickly [82].
It can be stated that the surface coating of the product reduces the passage of water molecules and improves the durability of the product. It is believed that the bioactive phytochemical compounds (phytosterols, tocopherols, squalene, stanols, sphingolipids, phospholipids, chlorophylls, carotenoids, phenols, and volatile compounds) are present as active antioxidant compounds in the composition of mastic gum [14][15][16][17][18][19]. The decrease in peroxide and thiobarbituric acid index can be attributed to the presence of triterpenics. The simultaneous presence of antioxidant compounds may create a boosting state and increase total antioxidant activity.
On the other hand, studies have confirmed its efficacy as a functional food for gastrointestinal disturbances or several modern lifestyle-related diseases of consumers who have been using mastic daily for many years in the form of chewing gum for oral health, or in powder or capsule [83][84][85].
Therefore, using mastic gum as a coating material could have double benefits, extending the shelf-life of a food product and, at the same time, promoting health benefits associated with its compounds. This could lead to a decrease in their nutritional content because the products with a protective layer can be stored longer.

Conclusions
The results of the present study indicated that mastic gum coating used in four different concentrations (0.5%, 1%, 1.5%, and 2% w/v) containing different bioactive compounds was effective significantly (p < 0.05) against moisture absorption and oxidative reactions, total yeast and mold growth, and Aspergillus species development in peeled and coated fresh almonds, compared to uncoated fresh almonds as the control sample, during 4 months of storage, packed at room temperature (25-27 • C) inside a cabinet at 90% humidity. By increasing the concentration of mastic gum, the protection against environmental factors during the storage period increased.
It could be concluded that mastic gum coatings could be effectively utilized to extend the shelf life of peeled fresh almonds and other nuts (containing high fatty acids content), on the one hand, and promoting health benefits, on the other hand, due to its valuable bioactive components. To our knowledge, there is no information in the literature on the use of mastic gum neither as edible coating nor on coating fresh almonds using mastic gum as edible coating to maintain the quality of the product during storage.
Due to the increase in the oxidation potential of fats, it is of potential interest for those with increased sensitivity to oxidation.
In addition, existing products in the market with coatings are around twice as expensive as those without, and so far, the consumer has paid for these additional costs, not the supermarket. Therefore, an edible coating such as natural mastic gum will open new perspectives.
Mastic gum belongs to the next generation of bio-based materials, such as natural plant materials, as substitutes for materials from nonrenewable sources, replacing traditional petroleum polymers in several industrial sectors.

Data Availability Statement:
The data used to support the findings of this study are included within the article.

Conflicts of Interest:
The authors declare no conflict of interest.