Active Biopolymeric Films Inoculated with Bdellovibrio bacteriovorus , a Predatory Bacterium

: The objective of the present work was to evaluate novel active ﬁlms made with biopolymeric matrices as carriers of a living Bdellovibrio bacteriovorus HD100 strain, a predatory bacterium with antimicrobial potentials against pathogens. Biopolymer ﬁlms were prepared by a casting method using the following mixtures: collagen/sodium alginate/sorbitol (CA-S), collagen/sodium alginate/glycerol (CA-G), and tapioca starch/sodium alginate/glycerol (StA-G). The effects of the ﬁlm formulations on the viability of the B. bacteriovorus was investigated by using Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC), and Scanning Electron Microscopy (SEM). SEM showed that Bdellovibrio bacteriovorus morphology was not altered in the polymeric ﬁlms. FTIR spectroscopy provided information about the structural composition of the ﬁlms. CA-S showed less reduction in the viability of B. bacteriovorus after its entrapment; thus, CA-S proved to be a better agent for the immobilization and preservation of B. bacteriovorus to enhance its predatory activities during application against Escherichia coli. mers. The CA-S ﬁlm showed a T g = − 24.94 ◦ C ± 1.19 with a ∆ C p = 0.88 ± 0.05 J/(g · ◦ C), and T g = 40.84 ± 0.48 ◦ C with a ∆ C p = 0.16 ± 0.01 J/(g · ◦ C). The CA-G ﬁlm showed a T g = − 21.83 ± 1.08 ◦ C with a ∆ C p = 0.19 ± 0.03 J/(g · ◦ C), and a T g = 33.70 ± 1.60 with a ∆ C p = 0.13 ± 0.03 J/(g · ◦ C). The high degree of crosslinking between hydrolyzed collagen and sodium alginate in the CA-S and CA-G ﬁlms, together with the plasticizer during ﬁlm formation showed a decrease in the ﬁrst T g with respect to the T g of collagen anhydrous (82.51 ± 0.21 ◦ C), and a second T g allied to the T g of sodium alginate anhydrous (41.85 ± 1.47 ◦ C). Sodium alginate presented the following melting parameters: T 0 = 145.75 ± 0.01, T p = 145.84 ± 0.07, T c = 152.67 ± 0.4 ◦ C, and ∆ H = 7.09 J/g ± 0.14, which are lower than those obtained for the CA-S and CA-G ﬁlms, except for enthalpy of fusion. These results show a greater thermal stability of the ﬁlms containing both polymers than sodium alginate itself. In it was reported that the addition of sodium alginate to collagen ﬁlms the polymeric interactions in the ﬁlms. Regarding the CA-S and CA-G ﬁlms zoomed in thermal properties the type of plasticizer used; the CA-G ﬁlm melted at a higher temperature than CA-S, and CA-S showed the highest melting enthalpy.


Introduction
In recent years, active packagings for increasing the shelf life of foods has attracted much attention in the field of food preservation due to increased demand for foods that are free of synthetic preservatives [1,2]. Biological control of bacterial pathogens using Bdellovibrio bacteriovorus (B. bacteriovorus) and other bacteria [3] is a new alternative to preservatives, considering that synthetic chemical preservatives and additives should be avoided because many of these could cause health damage [4].
B. bacteriovorus is a deltaproteobacterium that preys especially upon other Gramnegative bacteria. B. bacteriovorus invade the periplasm of their prey, forming a structure called a bdelloplast [5]. Within the bdelloplast, B. bacteriovorus grows, multiplies, and finally lyses its prey, releasing progenies that can continue the attack [6]. As a prokaryotic predator, B. bacteriovorus has the potential to serve as an agent for the biocontrol of bacterial pathogens. Additionally, it is safe for humans as a therapeutic agent and found in abundance in healthy human beings [7,8]; however, for the effective use of B. bacteriovorus as a biocontrol agent in food packaging, it is paramount to keep it viable and stable.
The selection and development of a suitable encapsulating agent are important for the viability of B. bacteriovorus. Moreover, encapsulating agents of natural polymer origin can serve as adjuvants for the protection and stability of B. bacteriovorus when applied to control pathogens during food storage. The sources of natural polymers used for edible packaging films include gums (e.g., arabic, xanthan, gellan, and curdlan), plants (e.g., starch, cellulose, pectin, and gum acacia), animals (e.g., whey protein, collagen, keratin, and gelatin), algae (e.g., alginate and carrageenan), and fungi (e.g., chitin, pullulan, and scleroglucan).
In this study, the viability of B. bacteriovorus was tested in three of the natural polymers, and these include collagen, alginate, and starch. Collagen is a long and fibrous protein, which constitutes approximately 30% of the body's total protein mass in mammals. Collagen fibrils are present in tendons, skin, bones, cornea, ligaments, muscle, and dentin [9]. Various authors have reported collagen films derived from various sources, including bovine [10], cow [11], and fish skins [12]. Furthermore, collagen films have wide applications because of their good flexibility and strong mechanical properties due to the self-aggregation and crosslinking of collagen fibrils [10]. Sodium alginate is a natural polysaccharide derived from brown algae, which when combined with collagen, forms films with suitable mechanical and barrier properties [13]. Sodium alginate has application in food packaging industries. Starch is a natural polymer of plant origin that has found its application in the food industries. For instance, L. acidophilus has been successfully encapsulated in cassava starch edible films [14]. In anticipation of finding alternative preservation methods to combat the proliferation of food spoilage and pathogenic bacteria, B. bacteriovorus, a predatory bacterium, has been recently considered as a biocontrol agent.
To the best of our knowledge, there has been no report on the use of biopolymeric films for immobilization of B. bacteriovorus. This work is focused on the production of active biopolymeric films, and the paper is aimed at: (1) trapping the B. bacteriovorus HD100 strain using the following mixtures: collagen/sodium alginate/sorbitol (CA-S), collagen/sodium alginate/glycerol (CA-G), and tapioca starch/sodium alginate/glycerol (StA-G); (2) determining the entrapment yield of the B. bacteriovorus HD100 strain in these formulations; and (3) characterizing the mechanical and thermal properties of biopolymeric films.

Biopolymers, Media, and Bacterial Strains
Sodium alginate and hydrolysed collagen were acquired from D'xilou (Mexico city, Mexico). Tapioca starch was acquired from Bob's Red Mill Natural Foods (Milwaukie, OR, USA).Sorbitol, glycerol and all media were acquired from Sigma-Aldrich ® (St. Louis, MO, USA). Bdellovibrio bacteriovorus HD100 strain and Escherichia coli DH5α were provided by the CBG-IPN (Reynosa Tamps, Mexico).

Inoculum Preparation
The host bacteria, E. coli DH5α, was cultured in 40 mL of LB broth at 37 • C under agitation for the duration of a night. After that, the E. coli culture was centrifuged at 3000 rpm and the pellet was washed with 10 mL of HEPES buffer containing 3 mmol/L of CaCl 2 ·2H 2 O and 2 mmol/L of MgCl 2 ·6H 2 O (pH 7.4). The predatory bacteria of the B. bacteriovorus HD100 strain were cultured in the presence of the host bacteria on DNB agar using the double agar overlay method [15]. After that, a lytic plaque was obtained and purified by repeating the double agar overlay method.
The purified plaque was inoculated with 50 mL of E. coli contained in HEPES buffer and after predatory activity, 40 mL of the cleared lysate were filtered (MF-Millipore™ of 0.45 µm) in order to purify B. bacteriovorus. Purified B. bacteriovorus cells in co-culture with washed E. coli cells were incubated at 30 • C under agitation for 7 d. B. bacteriovorus was purified again, and was concentrated by centrifugation (15,700 rpm) and washed twice with 50 mL of phosphate-buffered saline. The resulting concentration contained 1 × 10 8 PFU/mL of B. bacteriovorus cells, which were used to inoculate biopolymeric films.

B. bacteriovorus Entrapment in Biopolymeric Films
Inoculation of B. bacteriovorus in the films was carried out by the casting method; three different biopolymeric films were prepared. Biopolymeric films were prepared by the casting method using two plasticizers and combined polymers: a collagen-sodium alginate film plasticized with sorbitol (CA-S), a collagen-sodium alginate film plasticized with glycerol (CA-G), and a tapioca starch-sodium alginate film plasticized with glycerol (StA-G).
For CA-S, 25 g of hydrolyzed collagen, 2.5 g of sodium alginate, 17.5 g of sorbitol, and 230 mL of distilled water were used. For CA-G, 25 g of hydrolyzed collagen, 2.5 g of sodium alginate, 10 g of glycerol as a plasticizer, and 230 mL of tap water were used. For StA-G, 8.75 g of tapioca starch, 3.75 g sodium alginate, 10 g of glycerol as a plasticizer and 230 mL of distilled water were used. The amounts chosen for starch formulation were based on the results reported in [13].
All of the prepared mixtures were homogenized by mechanical stirring at 110 • C for 20 min and later sterilized in an autoclave for 15 min at 121 • C. The films were obtained by casting the suspension into Teflon moulds (11 × 21.5 cm 2 ). The filmogenic solution (10 mL) at 40 • C was poured into a Teflon mold, and subsequently a concentrated pellet of the B. bacteriovorus HD100 strain (1 × 10 8 PFU/mL) was added to the solution. The film was dried out in a ventilated oven at 37 • C overnight. The thin biofilms were removed from the molds and stored in desiccators until their use.

Scanning Electron Microscopy (SEM)
Bacteria trapped within the polymeric matrices were observed in a Dual-Beam SEM FEI Nova 200 (Nova NanoLab™, Tustin, CA, USA), using a low acceleration voltage of 3-5 kV and a chamber pressure of 0.60 mbar. The active biopolymeric films were fixed on aluminum stubs with double-faced adhesive tape. Magnifications at 1500×, 5000×, and 10,000× were obtained by image analysis.

Evaluation of the Entrapment Yield of B. bacteriovorus in the Biopolymeric Films
The viability of the predatory bacteria was quantified after drying the films by measuring the number of plaque-forming units produced. Dry films loaded with B. bacteriovorus HD100 were rehydrated in 5 mL of sterile distilled water for 10 min and were serially diluted ten-fold. The diluted film solution was cultured on dilute nutrient broth agar medium (DNB) using the double-layered agar plating technique and E. coli DH5α as prey. The number of plaques that developed after 72 h from the rehydrated filmogenic solution was recorded and expressed in PFU/mL. The entrapment yield (EY) of biopolymeric films was calculated as follows: where N 0 is the inoculum concentration in PFU/g of the film and N (PFU/g) is the viable cells' concentration extracted from the films after employing the casting method. The total count of the viable cells was analyzed in triplicate. The statistical analysis was carried out using the Mann-Whitney U test (p < 0.01) by using SigmaPlot 14.5 software (SYSTAT, San Jose, CA, USA).

Lytic Activity of B. bacteriovorus HD100 against E. coli DH5α in Co-Culture Assay
The predatory activity of Bdellovibrio bacteriovorus HD100 to reduce the concentration of Escherichia coli DH5α in the co-culture was assessed after 24 and 48 h. The reduction of E. coli was determinate by CFU/mL count as follows: serial dilutions (10 2 to 10 9 ) were prepared from one milliliter aliquot collected from the co-culture (Section 2.2) after 24 and 48 h. Subsequently, 100 µL of each dilution were spread-plated onto LB agar to determine the CFU/mL of the respective prey cell cultures. In addition, the double-layered agar plating technique was used to count the PFU/mL of B. bacteriovorus HD100. The measurements were performed in triplicate.
Lytic activity of predatory bacteria was observed in the form of clear areas in plaques containing a lawn of prey cells; photographs were captured by using a smartphone camera. Molecular interactions in biopolymeric films were studied by FTIR spectroscopy using a Jobin-Yvon LabRam HR800 spectrometer (HORIBA Scientific, Piscataway, NJ, USA). The individual polymers and biofilms were analyzed over the range of 4000-4500 cm −1 , with 4 cm −1 of spectral resolution and 40 scans per sample, using an attenuated total reflectance objective.

X-ray Diffraction (XRD) Analysis of the Biopolymeric Films
The XRD analysis of biopolymeric films was determined on a Rigaku Miniflex 600 diffractometer (Rigaku ® , Tokyo, Japan). The instrument was operated at 40 kV and 15 mA with CuKα radiation (λ= 1.54 Å) in 2θ sweeping from 0 • to 60 • with a step size of 0.01 and 0.03 • /min. The degree of crystallinity (% Cr) was determined according to the method described in [16]. OriginPro 10 software (OriginLab Corporation, Northampton, MA, USA) was used to analyze the spectrum.

Thermal Analysis by Differential Scanning Calorimetry (DSC)
The melting temperature (T p ), the melting enthalpy (∆H) in J/g, and the glass transition (T g ) temperature of casting films, as well as the T g and ∆C p of film-forming polymers, were determined using a Discovery DSC calorimeter (TA Instruments, New Castle, DE, USA) calibrated with indium [17]. Samples of approximately 2.1 mg were weighed into Tzero ® Hermetic Aluminum pans, and after that the samples were subjected to a cooling program from 25 to −75 • C and to a heating program from −75 to 170 • C (10 • C/min). The analysis was performed in triplicate using the software TRIOS 5.1.1 (TA Instruments, New Castle, DE, USA).

Mechanical Analysis of the Biopolymeric Films
The biopolymeric films were stored in a desiccator at 57% relative humidity for 72 h before analysis. Subsequently, the samples were cut at dimensions of 100 × 10 mm (ASTM D882-02 method). The tensile strength (MPa), the elongation at break (%), and the Young's modulus (Mpa) were determined using a TA.TX2i texture analyzer (Stable Micro Systems, Surrey, UK) following the methodology of a previous work [18]. The analysis was performed by tenfold. Figure 1 shows FTIR spectra of the individual polymers. Tapioca starch exhibited absorption band characteristics at the band of 3692-3000 cm −1 , where the maximum absorption was found at 3300 cm −1 ; this band is associated with the tensile stress of the vibrational free bonds of inter-and intramolecular hydroxyl groups of the anhydroglucose units. The peaks observed at 2923, 1411, and 1350 cm −1 are attributed to CH stretching vibrations and bending between methylene groups [19]. The peak at 1635 cm −1 is associated with the bending vibration of H 2 O and H-O-H, which presumably originates from tightly bound water present in the starch granules [20,21]. Three characteristic peaks of -C-O-stretching were observed in the fingerprint region of the starch spectrum. The peak at 1152 cm −1 is associated with the C-O bond stretching of the C-O-H groups, and the peaks at 1080 and 1027 cm −1 are attributed to covalent bond stretching of the C-O-C group in the anhydroglucose ring [22].

Molecular Interactions
In the case of the sodium alginate spectrum, the band located between 3692 and 2974 cm −1 was associated with the -O-H and C-H aliphatic chain stretching vibrations, respectively [23]. The signal observed at 1597 cm −1 corresponds to the asymmetric stretching vibration of the carbonyl bond -C=O, while that peaks of 1411 and 1025 cm −1 are attributed to carboxyl stretching (-COO and -C-O).
The observed spectrum for collagen is similar to that reported for buffalo skin [24]. The band located between 3684 and 3113 cm −1 is attributed to the N-H stretching vibration of Amide A, 1635 cm −1 (primary amide), which is associated with stretching vibrations of carbonyl groups (C=O bond) along the polypeptide backbone, and the 1529 cm −1 (secondary amide) signal results from N-H bending vibration, coupled with the stretching vibration of C-N. The signal at 1243 cm −1 (tertiary amide) is associated with C-N stretching, and the NH peak is related with the triple helix structure of collagen.  Concerning the StA-G film, spectra showed bands corresponding to starch, sodium alginate, and glycerol; a strong signal was observed at 1025 cm −1 assigned to -C-O groups ( Figure 2). This suggests that hydroxyl and carboxylate groups from sodium alginate and tapioca starch form a very strong intramolecular hydrogen bonding with glycerol. In the spectra of sodium alginate, the bands at 1597 and 1411 cm −1 were displaced to 1610 and 1413 cm −1 , respectively. The observed change at a higher wave number value shows a reduction in bond strength and stretch frequency. The band associated with stretching vibrations (3692 and 2974 cm −1 ) of sodium alginate was reduced in the StA-G sample (3671 and 2997 cm −1 ). The observed changes suggest intermolecular attraction through the formation of hydrogen bonds between groups in sodium alginate (-COO − ) and groups in tapioca starch (-OH).

Thermal Analysis by DSC
The thermal analysis plays an important role in determining the thermal stability and mechanical performance of films. Figure 3 shows the T g of the biopolymeric films manufactured by the casting process, and Figure 4 shows the T g of film-forming poly-mers. The CA-S film showed a T g = −24.94 • C ± 1.19 with a ∆C p = 0.88 ± 0.05 J/(g· • C), and T g = 40.84 ± 0.48 • C with a ∆C p = 0.16 ± 0.01 J/(g· • C). The CA-G film showed a T g = −21.83 ± 1.08 • C with a ∆C p = 0.19 ± 0.03 J/(g· • C), and a T g = 33.70 ± 1.60 with a ∆C p = 0.13 ± 0.03 J/(g· • C). The high degree of crosslinking between hydrolyzed collagen and sodium alginate in the CA-S and CA-G films, together with the plasticizer during film formation showed a decrease in the first T g with respect to the T g of collagen anhydrous (82.51 ± 0.21 • C), and a second T g allied to the T g of sodium alginate anhydrous (41.85 ± 1.47 • C). Sodium alginate presented the following melting parameters: T 0 = 145.75 ± 0.01, T p = 145.84 ± 0.07, T c = 152.67 ± 0.4 • C, and ∆H = 7.09 J/g ± 0.14, which are lower than those obtained for the CA-S and CA-G films, except for enthalpy of fusion. These results show a greater thermal stability of the films containing both polymers than sodium alginate itself. In a previous work [10], it was reported that the addition of sodium alginate to collagen films increased the polymeric interactions in the films. Regarding the CA-S and CA-G films (see zoomed plot in Figure 3), remarkable differences in thermal properties were observed with the type of plasticizer used; the CA-G film melted at a higher temperature than CA-S, and CA-S showed the highest melting enthalpy. In the StA-G sample, the T g could not be detected during the DSC experiment (see Figure 3). Sodium alginate is a polysaccharide polymer, which contains two hydroxyl (OH-) segments and one carboxylate (-COO − ) segment in every repeating unit, and each anhydroglucose unit in starch contains three hydroxyl groups. The tapioca starch sample showed a T g = 53.65 ± 8.3 • C (see Figure 4); the starch was completely gelatinized and the enthalpy of fusion of the film was very small at 150 • C. Due to the dynamic range of the DSC we could not observe transitions greater than 160 • C; it has been reported [21] that incorporation of starch into sodium alginate films led to melting temperatures in the range of 219-226 • C.

XRD Analysis
The diffractogram patterns for each biofilm formulation and relative crystallinity are shown in Figure 5. X-ray diffraction patterns of biopolymeric films showed absorption peaks and an amorphous zone. The collagen-alginate films (CA-S, CA-G) showed a higher intensity in XRD peaks at 2θ = 21 • and 2θ = 40 • than the starch-alginate-film (StA-G). The diffraction peak at 2θ = 21 • is related to structural layers of collagen fibrils [25], and the broad peak at 2θ = 40 • is attributed to sodium alginate [26]. The type of plasticizer lightly affected the XRD pattern of the biofilms; the biofilm plasticized with glycerol showed an additional shoulder at 2θ = 31 • , indicating enhancement of the film's crystalline structure. Films plasticized with glycerol showed higher Cr than those plasticized with sorbitol.

Mechanical Properties
The results regarding the mechanical properties of the prepared biofilms are shown in Table 1. TS represents the maximum strength of a film against applied tensile stress, E represents the ability of a biofilm to stretch, and YM indicates the rigidity of a film [27]. It should be noted that the starch-sodium alginate biofilm plasticized with sorbitol was not analyzed in this study because the biofilm was brittle, thus inhibiting the analysis. The TS of the collagen-alginate biofilms was highest in the CA-S film plasticized with sorbitol (4.78 MPa) and lowest in CA-G film (0.96 MPa). This could be due to the higher enthalpy needed to break the interaction formed between polymer-sorbitol (5.446 J/g) than polymer-glycerol (4.6569 J/g).
It was observed that the use of sorbitol in the CA-S film resulted in a stronger network structure than when glycerol was used in CA-G; these results agree with those obtained in FTIR (see Section 2). Similarly, Elango et al. [28], showed that catfish collagen-sorbitol casting films had a higher TS (4.8 MPa) than those obtained for catfish collagen-pglycerol casting films (TS: 4.1 MPa). In a recent work [29], it was shown that sodium alginate can improve the mechanical properties of collagen, which tends to become brittle during storage. This research showed that sodium alginate strengthens the collagen film structure, and therefore we decided to use the sodium alginate-collagen mixture in this research. Comparatively, the performance of protein films against polysaccharide films using glycerol indicated that polysaccharide films (StA-G) have a higher TS than films containing protein (CA-G). These results agree with those of Wang et al. (2017) [13].
StA-G films showed the highest E (83%) followed by CA-G glycerol (E: 73%) and CA-S (E: 41%). The compatibility of glycerol with starch through hydrogen bonding reduced the intramolecular attractions between starch chains, thereby promoting the mobility of the polymer chains in the StA-G film. Although StA-G presented the best E, its performance was less than the TS (10 MPa) reported for polyethylene films [30,31]; for this reason a coating with kraft paper [32] is suggested to improve the mechanical properties of the films for its application as packaging material. The E of CA-S was lower than the value obtained for StA-G; however, its therapeutic characteristics suggest that CA-S may have biomedical applications, since collagen and sodium alginate have been widely studied for tissue regeneration [33][34][35].

Visualization of Bdellovibrio bacteriovorus HD100 Strain by SEM
The micrographs observed by SEM depict the integrity of B. bacteriovorus after its entrapment in biofilms. Figure 6 shows the rod-shaped B. bacteriovorus HD100 strain completely embedded in the film matrix, a single bacteria (Figure 6e), and a clump of bacterial cells that is enclosed in a red circle (Figure 6f). The size of the B. bacteriovorus HD 100 strain observed by SEM was approximately 1 µm in length, similar to the value of 0.8 µm observed for the B. bacteriovorus 109J strain [36]. A cross section of the CA-S film is shown in Figure 6b, where specimens with unaltered morphology were observed. In the micrographs, smoother surfaces were observed in StA-G than in CA-G and CA-S, indicating the higher homogeneity of the StA-G films. Contrarily, CA-S and CA-G showed some bumps in their surface (Figure 6d). These bumps could be attributed to undenatured collagen fibrils, which are between approximately 0.5 and 1 µm in length. A study [37] has shown that collagen fibril diameters are between 0.1 and 0.3 µm. Table 1 shows the cell density of B. bacteriovorus HD100 assessed by the number of plaques formed at 72 h in the plaque, and its EY in biopolymeric films. The PFUs produced by B. bacteriovorus cells extracted from the films are shown in Figure 7. The growing inhibition zone is due to the movement of the predator within the soft top agar; this is a prominent feature of predatory bacteria [38]. In the present work, all double layer agar plates exhibited an inhibitory effect against Escherichia coli DH5α (Figure 7). A significant variation (p < 0.01) in the viability of B. bacteriovorus HD100 was observed in all treatments; CA-S was more effective than CA-G and StA-G in maintaining the viability of the B. bacteriovorus HD100 strain. Therefore, the highest EY of 93.46% resulted for the film plasticized with sorbitol (CA-S). This result agrees with the work of Dashiff and Kadouri [39], who showed that high concentrations of glycerol can inhibit the viability of the B. bacteriovorus HD100 strain and predation of the oral pathogens Aggregatibacter actinomycetemcomitans and Eikenella corrodens. In a similar study, Bdellovibrio sp. strain F16 was encapsulated in gelatin microparticles by spray drying [40]. The cell density obtained in the present work for the CA-S film (see Table 1) was higher than the cell density (5.4 × 10 7 PFU/g) reported for B. bacteriovorus microcapsules [40] produced by spray drying at 140 • C (inlet temperature). In the aforementioned study, no encapsulation yield was reported for B. bacteriovorus.  The concentration of E. coli bacterial cells (CFU/mL) obtained after 48 h upon exposure to the predator in HEPES buffer is shown in Figure 8. A decrease in cell concentration was observed compared to the initial inoculum (p < 0.05), and after 48 h the concentration of E. coli decreased by 1.42 logs. The results presented in this test confirm that active films have potential application in food preservation in forthcoming work.

Conclusions
To the best of our knowledge, this was the first time in which B. bacteriovorus HD100 strain was trapped into three different biopolymeric films by a casting process. The best suitable biopolymer formulation for the entrapment of the B. bacteriovorus HD100 strain was CA-S, which protected and enhanced the survival of B. bacteriovorus HD100 compared to CA-G and StA-G.
The CA-S film showed the highest TS, as was supported by FTIR analysis. It showed new chemical interactions between sorbitol and biopolymers. Moreover, the CA-S film had the highest TS, and required the highest melting enthalpy.
B. bacteriovorus showed effective predatory activity against Escherichia coli DH5α; thus, biopolymeric films containing the B. bacteriovorus HD100 strain can be used as biocontrol agents against pathogens in packaging materials to increase the shelf life of food products, for instance.

Data Availability Statement:
The data used to support the findings of this study are available from the corresponding author upon request.

Acknowledgments:
The authors acknowledge the experimental support of the CNMN-IPN in the realization of the present work.

Conflicts of Interest:
The authors declare no conflict of interest.