Electrodeposited Biocoatings, Their Properties and Fabrication Technologies: A Review

Coatings deposited under an electric field are applied for the surface modification of biomaterials. This review is aimed to characterize the state-of-art in this area with an emphasis on the advantages and disadvantages of used methods, process determinants, and properties of coatings. Over 170 articles, published mainly during the last ten years, were chosen, and reviewed as the most representative. The most recent developments of metallic, ceramic, polymer, and composite electrodeposited coatings are described focusing on their microstructure and properties. The direct cathodic electrodeposition, pulse cathodic deposition, electrophoretic deposition, plasma electrochemical oxidation in electrolytes rich in phosphates and calcium ions, electro-spark, and electro-discharge methods are characterized. The effects of electrolyte composition, potential and current, pH, and temperature are discussed. The review demonstrates that the most popular are direct and pulse cathodic electrodeposition and electrophoretic deposition. The research is mainly aimed to introduce new coatings rather than to investigate the effects of process parameters on the properties of deposits. So far tests aim to enhance bioactivity, mechanical strength and adhesion, antibacterial efficiency, and to a lesser extent the corrosion resistance.


Introduction
The coatings are widely applied for different purposes in the national economies and households. The metallic and polymeric coatings as paints and lacquers are used for the protection of metallic ferrous and nonferrous constructions, in buildings and houses for long term anti-corrosion protection and esthetic effects. The hard coatings are applied to increase the wear resistance. Many functional coatings have been so far proposed and developed. Just recently, for example, gas-barrier thin film coatings for food and beverages [1], the coatings for automotive brake discs [2], tribological coatings [3], functional textile coatings [4], super-hydrophobic anticorrosion coatings [5], and cermet coatings for erosion-corrosion-protection [6] were investigated.
The various coatings have been applied for many years in medicine to improve the properties of the interface between implants and tissues, which determine the biocompatibility and healing time. In past years, the reviews on such functional coatings for dental implantology [7], biocompatible coatings for bone implants [8], ion substituted hydroxyapatite thin films [9], titanium implants polymeric coatings [10], ceramic coatings for osteoporotic bones [11], and pulse laser deposited animal-originated calcium coatings [12] can be found.
The coatings for biological applications, called biocoatings, may be made of metals, polymers, ceramics, and bioglasses, or can be composite coatings, co-deposited, or formed layer-by-layer (hybrid or The HAp and nanoHAp coatings were deposited by EPD on different substrates, for example on NiTi [39], Ti [40][41][42], oxidized Ti [43][44][45][46], and Ag [47]. The Mg-containing hydroxyapatite coatings were also obtained on Ti alloy by MEO with destination for dental materials [48]. For the same purpose, in [49], the HAp nanoparticles were formed on Ti-Nb-Zr alloys by PED followed previous oxidation to nanotubular oxide surface layer. The simultaneous precipitation and electrodeposition of HAp were claimed to be useful for dentistry and orthopedic applications [50]. The HAp coatings can be formed on porous surfaces (scaffolds). After deposition [51] of the Ca-P coatings on the 3D surface of Ti scaffolds made by selective laser melting, the coating morphology was found to depend on the distance from the surface. The top and the bottom surfaces of SLM-Ti scaffolds exhibited continuous and instantaneous nucleation, but the EPD processes at different depth of SLM-Ti scaffolds did not follow the surface processes because of the non-uniform distribution of the potential and the current inside porous structures.
As a treatment positively influencing adhesion, the presence of a nanotubular oxide layer on titanium was extensively applied. For example, the nanotubular surface with the tube length of 560 nm coated with HAp showed higher cell density, higher live cells, and more spreading of MC3T3-E1 cells than that growing on titanium plate surface [52].
The use of HAp-based composite coatings with antibacterial properties is an important research direction. In [53], the calcium phosphate coatings were prepared and then loaded with an antiseptic agent, chlorhexidine digluconate, by a single-step co-deposition. The coating was effective against Staphylococcus aureus and Escherichia. coli bacteria strains.
Among other metallic biomaterials, the stainless steels were the object of some investigations. In [54], the HAp coating was deposited on 316L stainless steel (SS) in the presence of a significant amount of H 2 O 2 by both the direct and pulsed current electrodeposition methods. In [55], the effects of temperature and H 2 O 2 content on the morphology, structure, and composition of the coating were confirmed. Surprisingly, in [56], the HAp coatings plated by ECD on 316L steel, but also Ti and Ti6Al4V alloy, increased corrosion rate, likely because of highly porous coating and crevice corrosion. The PED seems a more efficient method as shown in [57], in which the perfect coatings of biocompatible HAp were obtained at various current densities over the SS316 steel. In later research [58], the phosphate coatings, transformed to hydroxyapatite, had thickness from 300 nm up to 2 µm, depending on the deposition mode (continuous or pulsed reverse), and voltage or current parameters, and microstructure of the flake type demonstrating the spontaneous passivation under anodic polarization, and the corrosion potential improved with the increasing presence of hydroxyapatite phase in the coating. An interesting process to form bi-layer (hybrid) coatings [59], the nanoHAp and phosphorus-rich electroless nickel composite coating as an interlayer, was developed before electrodeposition of pure HA coating on stainless steel.
Another group of biometals deposited with HAp was CoCrMo alloys. In [60], the Ca-P coatings were obtained consisting of fine crystallized HAp. On the other hand, for such coatings [61], the coating was weakly hydrophilic, with the phase angle over 85 • .
The next group of biometals deposited with HAp coatings was Ni-Ti alloys. In earlier work [62], such coatings were developed to improve the cardiovascular stents with less restenosis than drug-eluting stents. In another study [63], a bipolar pulsed current was used for the electrochemical deposition resulting in the crystalline coating composed of pure HAp nanowalls. Thorough studies of microstructure [64] showed for PED of Ca-P coatings that plate-like and needle-like morphologies were formed in dilute and concentrated solutions, respectively, and HAp appeared at increasing the pulse current density. The coatings obtained in dilute solution showed the best biocompatibility, the highest cell density, and cell proliferation, explained by the stability of the plate-like coating in biological environments. In [65], the surface modification of the NiTi alloy was accomplished before deposition by anodic oxidation and subsequent heat treatment.
Magnesium alloys have become promising materials in the medical field, particularly in tissue engineering applications. In [66], the HAp coating, electrodeposited on AZ91D magnesium alloy and comprising of brushite, lowered the biodegradation rate of Mg alloy in SBF, being the main aim of use of such coatings. The deposited coatings on Mg-1Ca alloy demonstrated improved corrosion resistance in Hank's SBF [67]. In another work [68], three kinds of Ca-P coatings, brushite, HAp, and the most stable and corrosion-resistant fluoridated hydroxyapatite (FHAp) were fabricated by electrodeposition on a biodegradable Mg-Zn alloy. To effectively control the degradation of magnesium alloys, a uniform nano-hydroxyapatite (nanoHAp) coating was applied on AZ31 magnesium alloy using both direct and pulse voltage electrodeposition methods [69]. In similar research on nanoHAp coatings [70] obtained by PED on the porous Mg-2 wt.% Zn scaffold, the corrosion current density reduced from 8 to 0.15 mA/cm 2 . The next study on nanoHAp deposition [71] on Mg-2Zn scaffolds performed by PED and post-treatment with alkaline solution demonstrated better biodegradation behavior and biocompatibility, and adherence and proliferation of the MG63 cells when compared to an uncoated alloy. In a more recent report [72], four electrodeposition methods, i.e., the constant potential method, the pulsed potential method, the constant current method, and the current pulsed method, were evaluated. Using the constant current and the current pulsed techniques, smooth uniform coatings were obtained onto AZ31 alloy for both low and high-frequency pulse. Finally, in [73], the Ca-P coatings on Mg implants were subjected to hydrothermal treatment, anodization, and plasma electrolytic oxidation.
The HAp coatings were seldom electrodeposited on ceramics. In [74], HAp coatings, from 1 to 10 µm in thickness, were prepared on human enamel by electrodeposition. They exhibited an acicular morphology and had a tight contact with the substrate.
The HAp coatings were sometimes deposited on polymer surfaces and scaffolds. By the templateassisted PED, the calcium-deficient polycrystalline HAp nanowires and nanotubes were obtained, nucleating preferentially on gold nanoparticles polycarbonate membrane, as nanowires on smaller Au particles and as nanotubes on 400 nm nanoparticles [75]. HAp, carbonate apatite, and dicalcium phosphate dihydrate (brushite) were deposited on carbon/carbon (C/C) composites [76]. A uniform and dense nanoHAp coating with the nanorod-shaped structure were fabricated on carbon nanotubes (CNTs), applied to reinforce the coating [77]. The ultrasonic-assisted electrodeposition was attempted to prepare uniform and smooth hydroxyapatite HAp coating on the surface of the PLA/PVA filaments, which can be potentially applied as biodegradable bone scaffolds [78]. The crystal's growth stages of HAp went through spherical particles, plate-like, and needle-like crystals when electrodeposited from 15 to 60 min.

Substituted Phosphate Coatings
The substituted hydroxyapatites include fluoridated hydroxyapatite (FHAp) and some other inorganic ions substituting calcium ions. The main reason for such design is an expectation of antibacterial effects and better bioactivity. Different substrates and electrodeposition techniques were applied.
As concerns, the titanium alloys, the Sr, Mg, and Zn substituted hydroxyapatite coatings, achieved by PED, exhibited excellent corrosion resistance on Ti6Al4V alloy, in particular at the prolonged pulse off time [79] and strontium-substituted calcium phosphate coatings on Ti6Al4V alloy demonstrated the best osteoblast cells activity and the osteoclast cells proliferation at 5% Sr [80]. In [81], magnesium-doped HAp (MgHAp) was obtained on titanium with a previously formed nanotubular oxide layer, showing only slightly increasing the bond strength. For the strontium/copper substituted hydroxyapatite (SrCuHAp) coatings on titanium, Cu improved its antimicrobial properties, and Sr enhanced the biocompatibility [82]. In other work [83], strontium and manganese co-substituted hydroxyapatite (SrMnHA) crack-free and dense coatings on titanium were prepared by ECD resulting in the decrease in the corrosion current density, strongly hydrophilic surface and better cell morphology, adhesion, spreading, and proliferation, and expression of alkaline phosphatase (ALP) better than on HAp. In similar research [84], the presence of strontium in nanostructured SrHAp coating deposited by ECD was shown again to positively influence the behavior of cells on a titanium surface, i.e., cell viability, adhesion, cell morphology, and the cytoskeletal structure of bone marrow mesenchymal stem cells (MSCs). For the hydroxyapatite doped with silver and manganese (AgMnHAp), deposited on TiO 2 nanotubes [85], corrosion resistance decreased by almost two orders of magnitude, and an excellent antimicrobial efficacy was observed with a 100% reduction in viable cells. The lanthanum and copper substituted hydroxyapatite (La/Cu-HAP) coatings made on titanium by ECD demonstrated excellent biocompatibility and bioactivity [86]. The cobalt-substituted Ca-P coatings on Ti22Nb6Zr alloy [87], protected the surface against corrosion in the physiological environment and promoted the development of the cells. The most recently [88], the Ca-P coatings were developed by pulse current deposition onto Ti6Al4V alloy, and after doped with Zn 2+ , Mg 2+ , Sr 2+ , and Ag + ions showed better biocompatibility, but also lowered corrosion resistance compared to no-doped Ca-P coating or bare metal.
In the case of steels as substrates, the deposition of (Zn 2+ , Cu 2+ , and Ag + ) substituted FHAp coatings were obtained on the 316L steel by ECD to further enhance the antibacterial efficiency of FHAp [5].
For Mg alloys [89], PED in the presence of H 2 O 2 was applied to improve the corrosion resistance and bioactivity of (nano)FHAP coating on Mg-Zn-Ca alloy. A silicon doped calcium phosphate coating was obtained [90] on AZ31 alloy by pulse electrodeposition demonstrating slow degradation rate in SBF, an enhanced corrosion resistance, good cell growth, and enhanced cell proliferation of MG63 osteoblast-like cells, as well as increased activity of ALP. The magnesium-substituted coatings had excellent biocompatibility and no adverse effect. Recently [91], a Si-HAp coating was achieved by PED on an Mg-Zn-Ca alloy to improve biocompatibility, bioactivity, and osteoconductivity.
The substituted HAp coatings were also deposited on polymer substrates. Strontium and magnesium substituted Ca-P coatings were formed on C/C composites by PED, showing the flake-like morphology with a dense and uniform structure that could induce the formation of apatite layers and decrease the corrosion rate of the C/C composites in simulated body fluid [92]. In other research [93], nanocrystalline zinc HAp was prepared by ECD on a titanium-coated silicone provided bioactive properties based on the fibroblast ingrowth and limiting the number of viable E. coli.

Oxides' Coatings
The metal oxides for biological applications were seldom deposited. In [94], PED was applied to co-deposit iridium oxide and human plasma proteins. In [95], a novel electrochemical sensor of creatinine was constructed on the glassy carbon electrode. The graphene oxide (GO) and dopamine were transformed to polydopamine and reduced graphene oxide (rGO) film and finally decorated by PED with the nanoparticles Cu by cyclic voltammetry.

Polymer Coatings
The most applied polymer for electrodeposited biocoatings was chitosan. In [96], the chitosan layer was deposited by ECD on a previously formed porous TiO 2 film layer. It is noteworthy that there was an exciting attempt [97] of electrodeposition of bioabsorbable polymer on NP-eluting stent made of stainless steel. Chitosan-mussel protein coatings were proposed to decrease the biodegradation of Mg in body fluids [98]. Photo cross-linking copolymer based on methacrylates deposited by EPD decreased the release of nickel ions from NiTi reducing cytotoxicity inducing by nickel [99]. The polyether ether ketone (PEEK) coatings [100] demonstrated excellent wear resistance, more than two hundred times greater compared with that of the uncoated Ti-13Nb-13Zr alloy.

Ceramics-Ceramics Coatings
The highest group of such coatings is based on hydroxyapatite-phosphate composition with often some ceramics. In [101], a coating composed of HAp and Ca-P phosphate was synthesized over 316L stainless steel through PED showing an increased corrosion resistance and good bioactivity. HAp and HAp/ZrO 2 composite coatings were [102] electrodeposited on 316L stainless steel to improve electrochemical behavior and bonding strength. When adding ZrO 2 at a concentration of 10 g/L, the corrosion resistance was improved 30 times, and bonding strength between the coating and the substrate increased from 11.6 MPa in pure HAp to 20.8 MPa in composite coatings. Another approach [103] was a deposition of a composite coating comprised of HAp, Ca-P, and multi-walled carbon nanotubes (MWCNTs) on SS316 steel surface by PED showing increased the overall coating modulus of elasticity in the range of 6-10 GPa, a uniform and fast spreading of cells over the coating surfaces and five times higher corrosion resistance.
The Hap-silicate coatings were also developed. In [104], such porous composite coating was obtained demonstrating higher bond strength and better corrosion resistance, and enhanced proliferation of MC3T3-E1 osteoblast cells grown on the HAp/CaSiO 3 coating compared to pure HAp. More complex [105] the electroplated zinc-doped hydroxyapatite-silicate coatings presented porous structure, higher corrosion resistance than bare Ti, and improved cell morphology, adhesion, spreading the proliferation of the MC3T3-E1 cells, and expression of ALP alkaline phosphatase comparing to HAp coating.
The nanocomposite coating comprising of calcium titanate, sodium titanate nanotubes, and rutile was prepared on a titanium substrate by a combination of ECD and chemical post-treatment [106] resulting in similar adhesion strength and increasing hardness and corrosion resistance were increased.
The zinc-halloysite nanotubes/(Sr 2+ , Sm 2+ ) substituted HAp hybrid coating was obtained on Ti6Al4V alloy by the ECD. The enhanced corrosion resistance due to Zn nanotubular layer, significant in-vitro antibacterial efficiency and cell viability studies, and anti-corrosion stability in SBF was observed [107,108].
The coatings composed of HAp and graphene oxide (GO) [109,110] were deposited by ultrasoundassisted PED on the anodized heat-treated surface of the titanium bringing out the highest nano-hardness (3.08 GPa) and Young's modulus (41.26 GPa). Similar hybrid coating composed of the GO inner layer and GO/MgHAp outer layer was prepared on carbon/carbon composites [111] with the bonding strength between the coating and carbon composite over 80% higher than without the oxide.
Carbon nanotubes (CNTs) were also proposed as material for brittle HAp. In earlier research [112], the CNTs reinforced HAp on the Ti substrate demonstrated high nanohardness, enhanced corrosion resistance, and cell viability. In more recent work [113], the addition of CNTs increased nanohardness over 52% and Young's modulus over 41%, and 16% in the adhesion strength thanks to improving integrity, crystallinity, and decreasing Young's modulus mismatch of this coating with titanium substrate. Such coatings were also prepared on AZ31 magnesium alloy by ECD and EPD [114], resulting in decreasing seriously corrosion density from 44.2 to 0.7 µA/cm 2 . In [115], different carbon nanostructures, namely exfoliated graphene, RGO, and MWCNTs were co-deposited along with in-situ formed HAp-Ca-P phases. The MWCNTs were deposited together with HAP also by the EPD technique [116,117].
Some oxides were applied for composite HAp coatings. In [118], HAp-ZrO 2 -TiO 2 nanocomposite coatings were synthesized with new morphologies of electrodeposited HAp, such as micro-nanorods. The corrosion current density of coatings was equal to 0.01 µA/cm 2 while the bare substrate was 1.5 µA/cm 2 , and porosity was decreased from 46% for HAp coating to 6% for composite coating. A ZnO nanoprism/Zn substituted HAp hybrid layer was prepared on carbon fibers by a two-step electrodeposition method [119].

Ceramics-Polymer Coatings
A novel implant coating material containing graphene oxide and collagen, and HAp was fabricated on Ti16Nb electrooxidized alloy [122] with increased corrosion resistance, superhydrophilic property, antibacterial resistance, and adhesion strength. In other research [123], a novel poly(3,4-ethylene dioxythiophene) based nanocomposite coatings with different contents of FHAp nanoparticles on a Ti-Nb-Zr alloy were developed demonstrating enhanced hardness, a contact angle, and corrosion protection than the pure polymer coatings. The fabricated nanocomposite coating supported the cell adsorption and proliferation of MG-63 cells and showed antibacterial performance. Another research showed [124] for a composite coating of HAp-polypyrrole, synthesized by PED over stainless steel, the increased corrosion resistance by ten-fold as compared to bare polymer coating and supported the growth of MG63 cells. Finally, in [94], a composite coating of iridium oxide and human plasma proteins was obtained by PED.
Some antibacterial nanocomposite coatings were developed [125] as the HAp-Ag-chitosan monolayers or multilayers containing HAp-chitosan and Ag-chitosan layers, with a thickness in the range of 0-20 µm. As assumed, the Ag + release rate from the Ag-chitosan layer can be reduced in the layered structure using a HAp-chitosan layer. The multilayer coatings provided corrosion protection of the stainless steel in physiological solutions. Similar biocomposite coating containing chitosan, silver, and hydroxyapatite was developed on anodized titanium substrate by ECD [126]-6-µm thick. The exhibited antibacterial activity against Gram-positive and Gram-negative bacterial strains was due to the synergistic effect of silver and chitosan, with no toxicity concerning MC3T3-E1 cells.
The electrodeposition of a chitosan/layered double MgAl hydroxides was applied to obtain hydro-membranes for protein release triggered by an electrical signal [127]. Chitosan was also co-deposited with HAp, ZNHAp, and MWCNTS [128].
Another approach based on HAp was attempted by the addition of the poly-l-lysine (PLL) + 3,4-dihydroxy benzyl aldehyde (DHBA) and TiO 2 [129] or PEEK [130]. The octadecyltrichlorosilane layer on HAp coatings was shown to be effective in preventing the acid corrosion of dental samples [131].

Metal-Ceramics Coatings
The nickel-MWCNTs coatings were co-electrodeposited [132]. The chemically shortened nanotubes were behaved as inert particles and embedded into the nickel matrix, while the long functionalized nanotubes showed metallic behavior, and during electrodeposition, they were incorporated into the nickel matrix. The hydroxyapatite-zinc coating was attained [133] using ECD possessing excellent adhesion to the substrate. In [134], a two-stage electrochemical synthesis method was used to prepare antimicrobial Ag-HAp composite coatings. In the first stage, a titanium substrate was coated with HAp by ECD, and in the second stage, silver nanoparticles were deposited onto the HAp layer through electrochemical reduction of aqueous Ag + to Ag 0 . Similar research was described by Bartmanski et al. for nanoAg [135,136] and nanoCu [137]. The coating composed of nanoHAp, MWCNTs, nanoAg, and nanoCu were developed to obtain antibacterial efficiency, high adhesion, and mechanical strength of coatings [138] also with additions of nanoAg and nanoCu. The ultra-fine Pd-Ag-HAp nanoparticles electrodeposited on protruded TiO 2 layer [139] were considered as plausible for dental implants as increasing their biocompatibility.
The influence of the silver nanoparticle (AgNPs) concentration in solution on the electrodeposition of Zn-Cu/AgNP composite coatings was studied by PED [140]. Antimicrobial tests revealed 95%-100% inhibition of bacterial growth after 10 min of contact for the Zn-Cu/AgNP coating with an AgNPs content of 0.28 wt.% against the E. coli.

Metal-Polymer Coatings
In [141], composite coatings consisting of polypyrrole (PPy) and Nb 2 O 5 nanoparticles were obtained by ECD on 316L stainless steel resulting in enhanced hardness, superior biocompatibility and enhanced corrosion protection as compared to pure PPy coatings. Other research [142] developed the polyethyleneimine film decorated with gold nanoparticles, electrodeposited on glassy carbon electrode, and forming the platform adequate to immobilize proteins for applications as a tool in biotechnology.

Polymer-Polymer Coatings
In [143], the alginate/chitosan layer-by-layer composite coatings were prepared on titanium substrates. No cytotoxicity was observed, and the alginate coating was more beneficial for cells' growth than chitosan coating. More recently [144], bioactive composite coatings based on polypyrrole (PPy)/chitosan were obtained by in situ electrochemical polymerizations with enhanced surface hydrophilicity and enhanced protective performance compared to pure PPy. Recently, partly biodegradable coating composed of chitosan and Eudragit (copolymer of methacrylic acid used for controlling drug release) was developed [145]. The gallium-modified chitosan/poly(acrylic acid) bilayer was proposed for titanium dental implants to prevent their failures [146]. Polymerized microemulsions of PMMA and soy lecithin on 316L stainless steel were also elaborated [147].

Effects of Component(s) on Properties of Biocoatings
The coatings used or developed for medical purposes can be made of different materials. That is determined by existing specific needs or, more often, by a demand to increase biocompatibility and bioactivity, and implement antibacterial efficiency. Therefore, the coatings are in a great number designed for metallic implants made of stainless steels, titanium, NiTi alloys.
The one-component metallic coatings were proposed for particular applications, mainly for construction of biosensors and, only in single cases, for others such as the creation of ferromagnetic coatings or as additional layers. This main application is related to the dependence of electrochemical potential of metals (half-cells) on the environmental signals like pH, temperature, and oxygen or hydrogen amount. The use of some oxides as single layers was scarce.
The ceramic coatings, in the past, were developed as simple phosphates (Ca-P: triphosphate (TCP), octacalcium phosphate (OCP), hydroxyapatite (HAp), and others). However, such coatings, even cheap and of good bioactivity, were prone to brittle cracking, often weakly adjacent to the base, and did not prevent the development of biofilm. Four solutions were then proposed: to modify the chemical formula of phosphate, to substitute phosphates by other substances, to create the composite HAp-based coatings, or to look for the best deposition technology and process parameters (as discussed in next section).
Among substituted phosphates, the fluorohydroxyapatite in which a fluoro group substituted a hydroxyl group, was often investigated. Besides, the hydrogen atoms were substituted by several metallic elements such as Zn, Sr, Cu, Ag, and Mn, mainly for titanium made implants. The attempts to decrease the solubility rate of biodegradable Mg alloys for implants by the development of SiHAp should also be noted.
Among one-component polymeric coatings, they were used for different purposes: for example, chitosan for increasing the bioactivity or to decrease the Mg degradation, methacrylates for prevention of Ni release from NiTi alloys, and PEEK as an anti-wear coating for biopairs.
The one-component coatings are usually relatively cheap, but their applications are limited as the obtained surface properties are not fully satisfactory. Therefore, the two or three or even four-component coatings were extensively developed.
The mostly applied are coatings composed of two ceramics such as HAp (or, more and more often, nanoHAp), silicates, oxides (mainly reduced graphene oxide rGO), CNTs (mainly MWCNTs), sometimes other substances as BN or halloysite. These coatings often demonstrated enhanced bioactivity and hardness, but their resistance to cracking, adhesion, and antibacterial action could be acknowledged as satisfactory only in part.
The promising direction is developing the metal-polymer coatings, which are usually based on HA with additions of chitosan, collagen, polypyrrole (Py), and others, sometimes implemented also with oxides. Such coatings may be well adjacent to the surface, stronger compared to pure ceramic coatings, and possess better biological properties. This research direction seems not so far developed, but very promising.
Other composite coatings were less investigated. It noteworthy that the metal-ceramics coatings based on nanoHAp implemented with antibacterial nanometals Ag and Cu were used for orthopedic and dental implants. Among the polymer-polymer coatings it is worth noting the appearance of several biodegradable or protective coatings.
Summarizing, there are two most promising development directions of research coatings. The first scientific goal is to create the coating highly bioactive, biocompatible, of high adhesion to both orthopedic and dental implants, but also resistant to corrosion and mechanical stresses, and antibacterial. Such coatings could be based on (substituted or no) nanoHAp and may include several other components: polymer for better crack resistance and additional biological properties (chitosan mainly recommended as a cheap and efficient component), nanoAg (or AgHAp, SrAgHAp, ZnAgHAp, and MnAgHAp). The second objective, less developed even if very promising, is to create the coatings with some specific functions as smart coatings for biosensors and long-term antibacterial protection, thin multifunctional coatings for stents, and wear-resistant coatings. The effect of used material on coating properties and possible applications is then highly significant, and the influence of applied technology, as demonstrated in other sections, is sometimes important and in other cases rather minor. Figure 1 demonstrates the electrodeposition method made (EDM) biocoatings. They have been divided at first according to whether they are on or multicomponent coatings, and then they have been listed based on the main materials.
Coatings 2020, 10, x FOR PEER REVIEW 10 of 33 ZnAgHAp, and MnAgHAp). The second objective, less developed even if very promising, is to create the coatings with some specific functions as smart coatings for biosensors and long-term antibacterial protection, thin multifunctional coatings for stents, and wear-resistant coatings. The effect of used material on coating properties and possible applications is then highly significant, and the influence of applied technology, as demonstrated in other sections, is sometimes important and in other cases rather minor. Figure 1 demonstrates the electrodeposition method made (EDM) biocoatings. They have been divided at first according to whether they are on or multicomponent coatings, and then they have been listed based on the main materials.

Electrocathodic Deposition (ECD)
Among the electrodeposition methods, the electrochemical deposition [16], also called (ECAD) or cathodic deposition, seems the most popular. The crucial determinants of microstructure and

Electrocathodic Deposition (ECD)
Among the electrodeposition methods, the electrochemical deposition [16], also called (ECAD) or cathodic deposition, seems the most popular. The crucial determinants of microstructure and properties of coatings include an electrolyte composition, voltage or current, depending on electrodeposition mode, and process time. Some research designs and results are described below. The specific determinants for many investigations are shown in Table 1 for one-component metallic, ceramic and polymer, and composite coatings.   RT-room temperature. Empty spaces mean that no data have been reported in references.

Effect of an Electrolyte Composition
As shown in Table 1, the regular baths for the deposition of Ca-P compounds contained such salts as Ca(NO 3 ) 2 , CaCl 2 , CaSiO 3 , and (NH 4 )H 2 PO 4 , often H 2 O 2 , more seldom KH 2 PO 4 . The typical concentration of calcium salt was 0.042 mol/L and 0.025 mol/L of (NH 4 )H 2 PO 4 . Hydrogen peroxide was added in concentration from 2000 ppm to even 2% [61]. As concerns the other additives, in [114] the addition of 1% MWCNTs to HAp showed the most positive effect on corrosion resistance.

Effect of Deposition Potential
In deposition performed under constant voltage, its value was usually between 1 and 3 V. The impact of potential was investigated in a few works. In [58], at 1 V of voltage, 300 to 400 nm thick flake type of deposition with random orientation was observed together with flakes either parallel or perpendicular to the coating surfaces, and under 2 and 3 V potential, an average thickness of flakes increased to around 2 µm. In [33,60], calcium phosphate coatings on the CoNiCrMo substrate were prepared by electrodeposition at different voltages and the moderate potential sample (−1.8 V vs. AgCl) they exhibited the most uniform coating. In [30], the calcium phosphates were electrodeposited on titanium and the coating electrodeposited at −2.0 V (SCE; Saturated Calomel Electrode) in the modified SBF containing CO 3 2− ions was the most bioactive, showing transformation into carbonate apatite similar to bone apatite. As stated in [152], the desired potential range for HAp electrodeposition may be comprised between −1.5 and −2.2 V (Ag/AgCl). These results are like each other and the effect of potential seems moderate.

Effect of Deposition Current
Such studies were carried out usually at current densities beginning well below 1 mA/cm 2 up to even 10 mA/cm 2 or more, as shown in Table 1. In [58], under constant cathode current density of 5 mA/cm 2 , the broom-like shape of deposition structure was observed, with flakes precisely perpendicular to the coating surfaces, with one after another originated from a single point with a wall thickness of around 400-500 nm, and spherical-shaped porous apatite structure with a diameter around 800-900 nm along with 50-100 nm pores throughout the outer peripheral surface appeared. At 10 mA/cm 2 current density, the continuous coating, and random flake type deposits were observed with around 1-2 µm wall thickness. In the ultrasonic-assisted electrodeposition of hydroxyapatite [78], when the current density increased from 2.5 to 7.5 mA/cm 2 , the grain size decreased from 414 to 265 nm. With the current density increasing from 2.5 to 5.0 mA/cm 2 , the Ca/P ratio rises from 1.53 to 1.58. Conversely, the Ca/P ratio drastically decreases to 1.25 at a current density of 7.5 mA/cm 2 .

Effect of Deposition Time
The result of deposition time was not often investigated, and applied values were diverse. In [34], by increasing the electrodeposition time from 1 to 30 min, the coating thickness increased, but also the surface morphology of the Ca-P coatings was greatly affected going from smooth to plate-like, featuring elongated plates, ribbon-like to finally sharp needle structures. The optimum electrodeposition procedures leading to both good cell-material interaction and sufficient mechanical properties could be achieved with relatively thin coatings produced at short electrodeposition times. The loading time increased the HAp crystallinity [149].

Effect of Deposition pH
The pH value, a rule, was buffered between 4 and 6. The HAp crystallization process was favored by an increase in pH from 2.5 to 6 [149].

Effect of Deposition Temperature
The deposition processes were conducted either at room temperature or, seldom, about 60-70 • C. In [149], with increasing temperature, the deposited hydroxyapatite was occasionally of plate-like shape, and the width and the length of the deposited calcium phosphates at 65 • C were more extensive than those at 55 • C.

Pulse Electrocathodic Deposition (PED)
The important determinants are shown in Table 2 for one-component metallic, ceramic and polymer, and composite coatings.   Cyclic voltammetry from 0.1 to −1.6 V vs.SCE - [140] RT-room temperature. Empty spaces mean that no data have been reported in references.
Hydroxyapatite, carbonate apatite, and dicalcium phosphate dehydrate were deposited on carbon/carbon (C/C) composites [76]. The supersaturation degrees of Ca 2+ and PO 4 3− changed the crystalline habit of HAp, led to large crystal size, and transformed crystal shape from belt-like to a plant-like structure. The H 2 O 2 was added to prevent hydrogen bubbles formation during electrodeposition and to favor better calcium phosphate crystals nucleation [152]. For HAp coatings deposited on NiTi alloy by PED, the morphology of the coating changed from needle-like to plate-like structure as the electrolyte concentration decreased about five times [64].

Effect of Deposition Potential
The deposition under constant voltage was usually run by cyclic voltammetry between to fixed potential value, come and back. In some reports [152], the pulsed mode was applied, in which the voltage was imposed for 60 s followed by break-time for 120 s. During the deposition of hydroxyapatite, carbonate apatite, and dicalcium phosphate dehydrate on C/C composites [76], the increasing voltage resulted in promoting bioactivity by altering the morphologies and phases. For silver nanoparticles in solution during the electrodeposition of Zn-Cu/AgNP composite coatings by cyclic voltammetry [140], the process was shown to occur through two stages with different energies. The first stage occurred in the potential range from −0.4 to −0.7 V vs. SCE and was mainly associated with the electrodeposition of a copper film, while the second stage corresponded to the bulk deposition of Zn-Cu/AgNPs and occurred from −1.4 to −1.6 V vs. SCE. The increasing HAp nucleation rate vis-à-vis increasing deposition potential was observed, and the presence of calcium orthophosphate phase was decreased along [58] with an increased rate of hydroxyapatite phase in the coating.

Effect of Deposition Current
Using the current pulsed methods, smooth uniform coatings were obtained onto Mg alloy when a current density in a specific range was used (10-30 mA/cm 2 ) [72]. The growth of particles was observed when low frequencies (50 Hz) and high frequencies (1000 Hz) were applied.
In [58], the effect of current parameters was observed. In other research [91], when Si-HAp coating was deposited on Mg-5Zn-0.3Ca alloy substrate by pulse electrodeposition, at a low current density of 20 mA/cm 2 a coarse and non-uniform coating was deposited, and at a high current density of 60 mA/cm 2 , high amounts of hydrogen gas at the interface were produced, and a non-uniform coating was formed. At the middle current density of 40 mA/cm 2 , nano-needle like coating was observed. For higher peak current density, the Ca-P coating consisting of needle-shaped crystals was formed with pores in between [54]. At lower current density of 0.5 mA/cm 2 a more compact uniform plate-like morphology was observed than those at a higher current density of 3 mA/cm 2 .
In [152], by the template-assisted pulsed electrodeposition method, the calcium-deficient hydroxyapatite (CDHA) particles in aqueous baths with hydrogen peroxide were used by both applying pulsed current density and pulsed potential in cathodic electrodeposition. Deposition in the membrane was successful only for higher values of current density, so a valid value of 120-250 mA/cm 2 was applied. In other research [101], a composite coating of hydroxyapatite and calcium hydrogen phosphate over 316L grade of stainless steel performed by PED at 10 mA/cm 2 demonstrated the highest weight percentage and crystallinity of hydroxyapatite phase and a continuous, faster and interconnected cell growth. For a composite coating of hydroxyapatite-polypyrrole, the coating deposited with moderate current density (10 mA/cm 2 ) seems to be the optimum one regarding the faster-interconnected growth of MG63 cells over the coating surface along with highest corrosion resistance and anodic passivation capability [124]. In [65], the electrodeposition at the higher current densities of 15 and 20 mA/ cm 2 increased the possibility of the hydroxyapatite phase formation in the coating rather than the other less stable calcium phosphate phases. The optimum conditions to create a uniform nano-hydroxyapatite coating on the Mg-Zn scaffold was found at 40 mA/cm 2 and 0.1 duty cycle [71]. Finally, in research [63], in which reverse current densities of 0.1 mA/cm 2 were applied, the porosity was increased by increasing the current density of the reverse pulse. Also, in this condition, the resulted film was composed of nanosized HA crystals. It seems that the reverse current step can influence the porosity and the structure of the coatings by the dissolution of the unstable phases, which are formed during the direct current stage of the bipolar pulse deposition. For the production of acceptable quality Ca-P coatings on Mg alloy, the current density should be between 10 and 30 mA/cm 2 [72]. In [49], the observed HAp morphologies consisted of particles, a mixture of particles and plate-like shapes, and entirely plate-like shapes as related to the increasing number of deposition cycles.

Effect of Deposition pH
The pH value was generally acidic. For deposited SiHAp coating at pH 5, the needle-like morphology, but the coatings deposited at pH 4 and 6, the plate-like morphology with micro-size thickness were observed [91].

Effect of Deposition Temperature
The applied were either room temperature or elevated temperatures, like 40-60 • C [53], 85 • C [70], or even 200 • C [25] temperatures were reported. In [91], tests were made at temperatures ranging from 25 to 100 • C, at temperatures up to 85 • C, the morphology of the SiHAp coating changed to the nano-sized needle-like blades. Table 3 illustrates the process determinants of several investigations performed by EPD. Chitosan + Eudragit acetic acid 10 and 30 V 1 and 3 min [145] PMMA + soy lecithin the microemulsion of coconut oil and water 1 mA (4-15 V) 30 min RT [147] RT-room temperature. Empty spaces mean that no data have been reported in references.

Effect of an Electrolyte Composition
The electrolyte composition affected biological properties. Galindo et al. [93] studied the effect of different Zn/Ca ratio on cytocompatibility and antibacterial efficiency demonstrating the best results at the 5% and 10% content of Zn in the carbonated hydroxyapatite (CHAp). In-vitro bioactivity of PEEK-HAp coatings was enhanced by increasing the amount of HAp particles, but such coatings had inferior adhesion to the substrate [130].
The electrolyte also affected corrosion resistance, adhesion, and wettability. The corrosion current, between 1 and 10 nA/cm 2 , was significantly higher for undecorated nanoHAp coatings and close to that of the substrate for decorated nanoHAp coatings [135]. The compact structure of hydroxyapatite with 20 wt.% silicon and hydroxyapatite with 20 wt.% silicon-1 wt.% multi-walled carbon nano-tubes coatings could efficiently increase the corrosion resistance of NiTi substrate [153].
The presence of nanosilver particles in the coating improved adhesive properties and reduce contact angle values [136]. Besides, the presence of nanosilver particles has a significant effect on homogeneity and quality of coating. In the presence of the silver nanoparticles, a smaller number of cracks and a smaller dimension of cracks were observed [40]. On the other hand, in [120] the increase in the amount of BN was insignificant as concerns the coating thickness.
The size of HPa seems to play an important role in biological behavior; the cell adhesion was better on the small-and middle-sized nanocrystals (SHA, MHA > LHA), which was interestingly opposite in cell spreading (LHA > SHA, MHA), and then the cell proliferation was again up-regulated on SHA and MHA [42].

Effect of Deposition Potential
In [42], the increasing potential at EPD of nanoHAp resulted in increasing coating thickness, the release rate of silver in SBF, resistance to scratch, but in decreasing hardness. On the contrary, in other reports [120], the voltage effect was insignificant as concerns the thickness.

Effect of Deposition Time
The increase in deposition time results in an increased thickness, decreasing hardness and increasing adhesion strength, and significantly decreasing the contact angle in wettability tests [137]. When EPD was made on the nanotubular TiO 2 surface, at a shorter 1 min EPD time, hydroxyapatite was deposited inside the nanotubes, between nanotubes, and also formed a thin layer over the nanotubes. At a longer 2 min, EPD time, the layer of HAp over the HAp-TiO 2 composite was thicker. The increasing thickness with deposition time was observed earlier [120].

Plasma Electrochemical Oxidation (PEO)
The plasma electrochemical oxidation, also called micro-arc oxidation (MAO), is generally assumed as a tool to generate a surface with high roughness affecting the adhesion of osteoblasts. However, PEO, when made in an electrolyte composed of different compounds, may significantly change the surface chemical and phase composition. Therefore, the attempts were made to obtain bioactive and well-adjacent to the substrate coatings using this approach. The significant part of such research was performed on titanium substrates. In [156], the coatings, consisting of HAp and titanium oxide, were produced on Ti6Al4V alloy in a solution containing calcium acetate and β-calcium glycerophosphate. The phases of anatase, rutile, TCP (Ca 3 (PO 4 ) 2 ), perovskite-CaTiO 3, and HAp were detected, their crystallinity more profound with increasing treatment time. The friction and wear resistance properties, and corrosion resistance of the PEO coatings were substantially improved compared to Ti6Al4V alloy. In similar research [157], the HAp/TiO 2 composite coating was prepared on a titanium surface. The flocculent structures were obtained during the early stages of treatment and, as the treatment period extended, increasing amounts of Ca-P precipitate appeared on the surface, and the flocculent morphology transformed into a plate-like morphology, and finally into flower-like apatite. The Ca/P atomic ratio gradually decreased, and the adhesive strength between the apatite and TiO 2 coating was improved. The search for a new complexing agent was described in [158]. The barium titanate coatings were also obtained [159] on Ti6Al4V alloy, and the coatings presented lower friction coefficient, higher wear, and corrosion resistance than uncoated Ti alloys. In [160], the coatings formed in a solution containing Ca and P achieved a thickness of 11.78 µm, and bond strength 33.69 MPa. Recently [161], two-layer hydroxyapatite CuHAp-TiO 2 composite coating was prepared on titanium in an aqueous electrolyte containing Ca, P, and Cu at a constant current density of 100 mA/cm 2 . Yu et al. [48] obtained Mg-containing hydroxyapatite coatings on Ti-6Al-4V alloy for dental materials by plasma electrolytic oxidation. The increasing Mg content in an electrolyte resulted in decreasing the irregularity of the surface, pore size, and the number of pores decreased as the Mg concentration increased.
After 20 min of process, Cu species incorporated into the TiO 2 as CuO and Cu 2 O, resulting in the formation of Cu-doped TiO 2 -based coating. Dziaduszewska et al. [162] found the best process parameters in a similar electrolyte as of 300 V for 15 min, at 32 or 50 mA of current value, which resulted in the proper microstructure, high Ca/P ratio, hydrophilicity, early-stage bioactivity. The coating mechanical properties, Young's modulus, and hardness were close to the values characteristic for bones, and adhesion was also better than at 200 V of voltage.
In addition to HAp coatings on Ti, also other substrates were covered with HAp for biological applications. The HAp coatings were grown on Ta samples by PEO at the potential in a range of 350 to 500 V, and treatment time from 60 to 600 s [163]. The formation of crystalline HAp was detected for samples treated over 180 s at 500 V. In [164], the coating was obtained by PEO on Zr in for process duration of 2 to 30 min. The increasing oxidation resulted in thicker and rougher coatings. The increasing oxidation resulted in thicker and rougher coatings. In [165], a hydrophilic surface was obtained by PEO on Mg with a biosynthesized hyaluronic acid and carboxymethylcellulose to improve bonding function.

Electro-Spark Deposition (ESD)
The ESD method was carried out on Ti6Al4V to deposit a layer on the steel substrates at the first step, and then the PEO process was employed to improve properties of the alloyed titanium layer at the second step [166]. The duplex coating consisted of α-Al 2 O 3 (corundum) and γ-Al 2 O 3 phases were formed after the PEO process while AlFe 3 , TiN, and AlTi 3 phases were detected in the ESD coating. In the next research [167], the HAp coatings were formed on steel again by ESD and PEO. The HAp-based surface was rough and porous, indicated a hydrophilic character, and improved bioactivity in SBF.

Electro-Discharge Method (EDM)
In [168], the hydroxyapatite coating was synthesized on the Zr-based bulk metallic glass surface to promote cell proliferation. The formation of the nanoporous coating of about 27.2 µm thick appeared, firmly adhered to adhesive failure at 132 N. A significant rise in cell viability was also shown. In [169], the surface modification of the β-type titanium substrate was made by EDT to incorporate MWCNTs and µ-hydroxyapatite (µHAp) powders in the dielectric medium to promote cell proliferation.

Electropolymerization (EP)
The electropolymerization is a method applied for some organic coatings. In [170], MWCNTspolyaniline composite films were prepared by in situ electrochemical polymerizations on the titanium from an aniline solution containing a small content of well-dispersed CNTs. Then these composite films were employed as a substrate for the electrodeposition of platinum nanoparticles forming the surface highly active for electrocatalytic oxidation of glycerol. In a more recent work [171], the polypyrrole (PPy)/chitosan composite coating on titanium was obtained by EP method showing enhanced microhardness and adhesion strength and higher corrosion resistance compared to the PPy coating.
Some specific environments were also utilized. In [172], the enzyme laccase was immobilized during the potentiostatic deposition of a thin polydopamine film (PDA) on carbon surfaces.

Effects of Electrodeposition Method on Properties of Coatings
The electrodeposition methods are important, even not prevalent (the sprayed coatings are the mostly applied for joint implants) for coatings on orthopedic and dental implants, maxillofacial implants, and stents. However, the choice of method depends on the designed coating, its predicted properties, and applications.
The most popular are electrocathodic deposition at either constant or pulse voltage/current, and as the third, electrophoretic deposition. The same coatings, mainly ceramic or composite ceramics-ceramics, ceramics-polymer, or ceramics-metal can be obtained by each of these methods. Therefore, the research was conducted on similar coatings by these methods even if there were few attempts to compare the coatings of the identical composition obtained by all EDM techniques.
ECD is the simplest and most developed technique in the past. However, it is excellent only for some metals. As concerns ceramics which cannot dissociate to ions, their deposition on the metal base is difficult, the surfaces are often non-stoichiometric and non-homogenous. Despite that, it is still the most popular deposition method, thanks to its simplicity, and it is widely used for Ca-P and HAp coatings, one or multicomponent layers. The applied voltage is between −1.5 to 2.5 V (SCE) for the Ti based materials and a little more for Mg alloys. The process is usually made under voltage control, and for current control, the current values are 0.1-3 mA/cm 2 . Deposition time depends on a used compound, and it is very short for metals, 15-60 s, and much longer for ceramics, For Ca-P and HAp coatings, even up to 1 h. The used bath is based mainly on two substances, Ca(NO 3 ) 2 and NH 4 H 2 PO 4 . The process parameters seem to play a less important role: the electrolyte composition is determined by the ionic components of designed coating, the voltage must be maintained within some limits, pH is usually neutral or slightly acidic. The ECD gives then no great possibility to adjust the properties of coatings by process parameters.
The pulse electrocathodic deposition (PED) gives more possibilities to control the coating. The voltage or, more seldom, current, is the obvious process parameter, but a duty cycle and number of cycles (deposition time) allow creating of previously design coating. The electric parameters are like those used under direct current, the duty cycle is between 0.1 and 0.5, deposition time reaches even 1 h.
The electrophoretic deposition is exceptionally plausible for no dissociated compounds such as ceramics and polymers. For the PED, process parameters such as duty cycle and the number of cycles need some preliminary tests. For the EPD, the most significant difficulty is to use the proper bath components (together with their contents in a bath) and solvent, in which a stable suspension is obtained and the species possess the proper charge. Ethanol, butanol, and isopropanol are mainly applied, but this list can likely be extended in the future affecting the process efficiency and coating adhesion and thickness. It is noteworthy that for the attempts to look for the most optimum electrochemical conditions: voltage was changed between 10 and 100 V, and once even it was 380 V, and deposition time was usually short, between 15 s and 2 min, sometimes longer. The use of such a short time allows obtaining the thin coatings, particularly suitable for coatings on metallic implants, for which an excessive thickness may result in a weak adhesion and failure. Therefore, it is a great advantage of EPD.
The critical factor for adhesion is the surface roughness. As a rule, it is adjusted by mechanical polishing, chemical etching, oxidation of Ti to nanotubular structure, and others. However, the micro-arc oxidation, MAO (called also plasma electrochemical oxidation, PEO) is the unique among electrodeposition methods, as it brings out the rough surface rich in biological elements due to high voltage oxidation in electrolytes containing Ca and P compounds. For that reason, it is commonly used for dental implants.
Other methods, such as electro-spark, electrodeposition, and electropolymerization techniques are less used. Despite that, they can be more intensively developed in the future as they allow creating the duplex coatings, nanoporous coatings, and polymer coatings.

Conclusions
The coatings proposed for medical applications are, to a greater extent, either ceramic or ceramic-ceramic or ceramic-polymer and, to a lesser extent, either polymer, metallic, and other composite coatings such as ceramic-metallic, ceramic-polymer, polymer-polymer, and others.
The most applied compounds are phosphates, and the most used substrates are titanium and its alloys and, to a lesser extent, NiTi alloys.
Three electrocrystallization methods are preferred for deposition of biocoatings such as direct and pulse electrocathodic deposition, and electrophoretic deposition. Plasma electrochemical oxidation, electro-spark, electro-discharge, and electropolymerization are not often applied.
The main process determinants include potential or current, and electrolyte composition, more seldom deposition time, pH, and temperature.
The current progress in biocoatings focuses on an enhancement of their bioactivity, biocompatibility (hydrophilicity), adhesion to substrates, mechanical strength, and corrosion resistance. Such properties are determined by microstructure and thickness of coatings, and characteristics of the interface.
Future research is to be directed towards the development of biocoatings possessing efficient and long term antibacterial efficiency, with no short and long term cytotoxicity, enhancing adhesion, proliferation, and viability of cells, well adjacent to the substrate, while being resistant to shear and compressive stresses, environmentally friendly, and economically justified.