Traditional Herbal Remedies with a Multifunctional Therapeutic Approach as an Implication in COVID-19 Associated Co-Infections

: Co-infection in patients with viral infection as a predisposing factor is less focused on during epidemic outbreaks, resulting in increased morbidity and mortality. Recent studies showed that patients with coronavirus disease 2019 (COVID-19) often have both bacterial and fungal co-infections. In this study, sputum samples of 120 OPD (outdoor patients) su ﬀ ering from respiratory tract infection (RTI) but negative for tuberculosis infection were collected with informed consent. Morphological, biochemical, and resistance criteria were used to classify isolates and to distinguish multidrug resistant (MDR) isolates, which were further classiﬁed on a molecular basis. We found that the isolates, including MDR strains, showed remarkable sensitivity against acetone and methanol extracts of Moringa oleifera, Adhatoda vasica , and Cassia ﬁstula . The results strongly conﬁrmed that multifactorial infections can produce MDR characteristics against antimicrobial drugs, which gave insight into the use of herbal drugs with their age-old traditional importance as having antiviral, antibacterial, antifungal, anti-inﬂammatory, and immunomodulatory e ﬀ ects. We conclude that apart from this, the anti-infective potential of these plants can be used in the future in the form of products such as cosmetics, pharmaceutical coatings, surface coatings, drug delivery vehicle coatings, and other bioengineered coatings for public use. Future studies are required to assess therapeutics for co-infective resistant strains and nosocomial infections with immune-enhancing e ﬀ ects, thereby promoting their function in holistic treatment and therapy of COVID-19 patients.


Introduction
Recently, a novel coronavirus named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was identified as the causative agent of a respiratory disease referred to as  is the most revered species [19]. Moringa oleifera (Lam) has high nutritional value and is considered a complete food due to its effective range of medicinal uses. The extracts of the leaves are considered to have biological properties and are normally found to differ with the type of solvent used to isolate the active compounds [20,21]. Moringa oleifera seed oil (MOSO) is considered for the production of organic surface coating resins, which showed excellent antimicrobial inhibitive properties with a thermal stability profile that is best use in the surface coating industry also [22]. Adhatoda vasica (Malabar nut) is a perennial plant that is well known in Ayurveda for its medicinal properties. Adhatoda vasica has been well known for its action against serious respiratory infections, with its leaves used for centuries as an expectorant with demonstrated antispasmodic activity against severe respiratory infections such as asthma and chronic bronchitis [23]. A previous study documented the efficient use of the methanolic extract of Adhatoda vasica in the production of antibacterial Adhatoda liquid soap [24]. One excellent use of Adhatoda vasica extract was shown by its inhibition efficiency of corrosion of steel surface [25].
This study shows the antibacterial and antifungal activity of the aforementioned well-known medicinal plant extracts on clinically relevant antibacterial and antifungal strains isolated from sputum samples. The results from this study implicate that medics can diagnose bacterial or fungal infections in COVID-19 patients with their drug sensitivity antibiogram, in turn providing them evidence-based detailed insight into specific prognosis and therapy, leading to marked precision in the prevention and control of infection-associated complications, which in turn contributes to lessening the number of deaths amongst COVID-19 patients [2]. Thus, the Indian system of medicine traditionally known as Ayurveda comes to the rescue at the forefront by providing all the necessary armamentarium, including a holistic approach in combating this global viral pandemic.

Sample Collection
Non-AFB (Non-acid fast bacilli) sputum samples were collected from 120 OPD (outdoor patients) suffering from respiratory tract infection with informed consent and ethical clearance from India. Samples were collected aseptically into well-labeled sterile wide-mouthed glass bottles. A patient's registering proforma with brief medical histories such as name, age, sex, hospitalization records within previous years, antibiotics prescribed, personal hygiene status, and other medication data, etc. were maintained.

Processing of Samples
Sputum samples were inoculated on different commercial media (HiMedia) such as nutrient agar, MacConkey agar, mannitol salt agar, Sabouraud dextrose agar, while chocolate agar and blood agar were prepared in the lab using sheep blood. Inoculated agar plates were incubated at 37 • C for bacterial isolates and 26 • C for fungal isolates for 24 to 48 h.

Identification of the Pathogens
The bacterial isolates were maintained in a nutrient broth (NB) at 37 • C while fungal isolates of candida spp. were maintained on Sabouraud dextrose agar (SDA) at 26 • C. The isolates were identified by cultural, morphological, gram-staining, and biochemical characteristics (according to Bergey's Manual of Determinative Bacteriology) [26], and the selected multidrug resistant (MDR) bacteria were further subjected to molecular identification by Sanger sequencing using the 3500XL genetic analyzer (Applied Biosystems, Waltham, MA, USA) for bacterial 16S rRNA and fungal 18S rRNA. The sequences obtained were used to create a phylogenetic tree using MEGA software. The sequences obtained were also submitted to the NCBI database with available accession numbers.

Antibiotic and Antifungal Susceptibility Test
Antibacterial and antifungal susceptibility testing was performed by Kirby Bauer's disc diffusion method according to the National Committee for Clinical Laboratory Standards guidelines of the Clinical Laboratory Standards Institute, USA. Antibiotic discs were placed on Mueller-Hinton agar plates, previously swabbed with the target bacterial isolate at a concentration of 10 5 CFU/mL (McFarland standard [27]), and were incubated for 24 h at 37 • C (bacteria) and 25 • C for Candida spp. Antimicrobial activity is defined as the diameter (mm) of the clear inhibitory zone formed around the discs [28].

Preparation of Plant Extracts
Fresh leaves of Moringa oleifera, Justicia adhatoda (Syn. Adhatoda vasica), and Cassia fistula were taken, identified by a taxonomist at the University of Allahabad. Fresh leaves from selected species of plants were washed preliminarily 2-3 times with tap water followed by distilled water and dried at 50 • C before being ground to a fine powder in a blender machine. Following this, 10 g of powdered material was soaked in a 100 mL solvent such as acetone and methanol and kept at 30 • C for 6-7 days in a rotary shaker. After that, it was filtered through Whatman filter paper No. 1. The filtrate was then evaporated using a rotatory evaporator to obtain concentrated, powdered extracts. All the extracts were concentrated as per need and stored in sterile vials at 4 • C for further use [29,30].

Test for Antimicrobial Activity of Selected Plant Extracts
The antimicrobial potential of the selected plant extracts was screened by the agar well diffusion method [31]. An inoculum suspension concentration of 10 5 CFU/mL (McFarland standard) was swabbed uniformly to solidified Muller-Hinton Agar (MHA) media. Holes of 5 mm in diameter were made in seeded agar using a sterile cork borer. An aliquot of 100 µL from each plant extract (100 mg/mL DMSO) was added into each well and was allowed to stand for 1h for proper diffusion, and thereafter incubated at 37 • C for 24 h (bacteria) and 26 • C for Candida isolates. The antimicrobial activity was evaluated by measuring the zone of inhibition (mm).

Determination of Minimum Inhibitory Concentration (MIC)
The minimum inhibitory concentration was assessed by the broth dilution method. The plant extracts were diluted serially to obtain concentrations of 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.90, and 1.95 mg/mL, respectively in separate tubes. Here, 100 µL of 10 5 CFU/mL (McFarland standard) of the tested strains of equal volume was transferred into each tube of nutrient broth and plant extracts, and incubated at 37 • C for 24 h respectively. Control tubes with one having nutrient broth was inoculated with bacteria as a positive control, and the other one containing nutrient broth with plant extract was used as a negative control. The test compounds that inhibit the visible microbial growth (no turbidity) at the lowest concentration after incubation of 24 h were taken as the MIC value [32,33].

Statistical Analysis
Data obtained from the study were analyzed using the Graph Pad Prism Version 5.0 for a Windows (Graph Pad Software) statistical package program. All experiments were performed in triplicates, and values were expressed as mean ± SEM (standard error of mean). Results obtained were statistically analyzed by using one-way Analysis of Variance (ANOVA).

Prevalence of Pathogens in Sputum Samples
To determine the pathogen in the sputum, 120 non-AFB sputum samples were examined in the present study. Out of 120 samples, 85 (70.83%) samples were reported positive, having different pathogens that caused respiratory tract infection. All isolates were analyzed biochemically, and the MDR pathogens were further analyzed by molecular sequencing as shown in Supplementary Table S1A,B. The sequences were also submitted to the NCBI database (Supplementary Table S2), and a phylogenetic analysis was performed (Supplementary Figure S1A-D). The accession number of pathogens whose sequences were submitted to NCBI are as follows: Pseudomonas aeruginosa KY471721, Escherichia coli KY471722, Morexella catarrahalis KY484918, and Candida tropicalis KY484919. All the isolated organisms showed pigmentation in nutrient agar media, which is shown in Supplementary Figure S2. The study reported sputum samples to be AFB negative, showing presence of pathogenic microbes such as Escherichia coli, Pseudomonas aeruginosa, Candida tropicalis, Moraxella catarrhalis, Staphylococcus aureus, Serratia spp., Enterobacter spp., and Candida spp. These isolates possess a demonstrated history of medical importance in causing severe infections to humans. The data of sputum samples from respiratory tract infection with the total sample size were recorded as 120, out of which 85 (70.83%) isolates were found to be positive. The percentage of occurrence of different pathogens amongst positive isolates is mentioned in Figure 1.
Windows (Graph Pad Software) statistical package program. All experiments were performed in triplicates, and values were expressed as mean ± SEM (standard error of mean). Results obtained were statistically analyzed by using one-way Analysis of Variance (ANOVA).

Prevalence of Pathogens in Sputum Samples
To determine the pathogen in the sputum, 120 non-AFB sputum samples were examined in the present study. Out of 120 samples, 85 (70.83%) samples were reported positive, having different pathogens that caused respiratory tract infection. All isolates were analyzed biochemically, and the MDR pathogens were further analyzed by molecular sequencing as shown in Supplementary Table S1A,B. The sequences were also submitted to the NCBI database (Supplementary Table S2), and a phylogenetic analysis was performed (Supplementary Figure S1A-D). The accession number of pathogens whose sequences were submitted to NCBI are as follows: Pseudomonas aeruginosa KY471721, Escherichia coli KY471722, Morexella catarrahalis KY484918, and Candida tropicalis KY484919. All the isolated organisms showed pigmentation in nutrient agar media, which is shown in Supplementary Figure S2. The study reported sputum samples to be AFB negative, showing presence of pathogenic microbes such as Escherichia coli, Pseudomonas aeruginosa, Candida tropicalis, Moraxella catarrhalis, Staphylococcus aureus, Serratia spp., Enterobacter spp., and Candida spp. These isolates possess a demonstrated history of medical importance in causing severe infections to humans. The data of sputum samples from respiratory tract infection with the total sample size were recorded as 120, out of which 85 (70.83%) isolates were found to be positive. The percentage of occurrence of different pathogens amongst positive isolates is mentioned in Figure 1.

Antibiotic Susceptibility Pattern of Isolates (Antibiogram)
All the isolated organisms were examined for antibiotic sensitivity, which showed marked differences in their sensitivity against different antibiotics, viz. broad spectrum to narrow spectrum (Supplementary Figure S3A-G). The pathogenic isolates, including some strains of Pseudomonas aeruginosa, Escherichia coli, and Moraxella catarrhalis, showed MDR characteristics ( Table 1). Strains of Staphylococcus aureus (gram positive) showed maximum resistance against vancomycin and erythromycin while appearing to be sensitive against the other antibiotics ( Table 2). The zone of inhibition in mm was measured for the isolated organisms against the different antibiotics and is shown as an antibiogram. Serratia spp. and Enterobacter spp. show maximum susceptibility against different antibiotics used. Candida spp. was only identified by gram staining, which showed gram-positive budding yeast cells, out of which one strain was further analyzed on a molecular basis and identified as Candida tropicalis, which showed partial resistance. Candida tropicalis was resistant against CLT and KTC, intermediate against NYT and FLC while sensitive against ITC and AMB antifungals ( Table 3). Table 1. Antibiotic susceptibility pattern of isolates in percentage is calculated according to the number of positive isolates present in the examined samples (S-Sensitive, R-Resistance).

Candida Tropicalis
CLT (10 µg Table 4. The antimicrobial activity of isolates along with MDR isolates was checked against acetone and methanol extracts of the selected medicinal plants. The broad-ranging antimicrobial activity with 100% sensitivity against the isolates, including MDR pathogens, was very well exhibited by the three selected genera of medicinal plants. The highest zone of inhibition (18.6 mm) was shown by the acetone extract of Cassia fistula against S. aureus, whereas for the methanol extract, the highest zone of inhibition (17.0 mm) was against Candida tropicalis. The methanol extract of Moringa oleifera gave more potent activity against different isolates than acetone extract did. Acetone and methanol extracts of Adhatoda vasica gave a wide range of antimicrobial activity against different isolates. Moraxella catarrhalis showed significant sensitivity towards all the 3 plant extracts. The antibiogram of the selected plant extracts is given in (Table 5).  The study shows that the antibacterial activity of acetone and methanol extracts of Moringa oleifera, Cassia fistula, and Adhatoda vasica is found to be effective against all the pathogenic isolates along with MDR strains, which is shown in Figure 2A-G.

MIC Values of the Plant Extract, Viz. Moringa Oleifera, Adhatoda Vasica, and Cassia Fistula, against the Isolated Pathogens
The selected plant extracts showed various degrees of antimicrobial activity against all the pathogenic strains. The acetone extract of Cassia fistula was the most effective against the microbial pathogens with low MIC values as compared to the other extracts. Acetone extract of M. oleifera gave a MIC range between 31.25 to 62.5 mg/mL, whereas methanol extract showed a MIC range between 31.25 to 125 mg/mL. The acetone extract of A. vasica gave MIC values, viz. 15.62 to 125 mg/mL, while methanol extract gave 31.25 to 125 mg/mL. The MIC values for C. fistula were the lowest, viz. 15.62 to 62.5 mg/mL for acetone and 7.81 to 62.5 mg/mL for methanol. The selected plant extracts showed MIC values in the range of 7.81 to 125 mg/mL against the isolated pathogens, which are presented in Table 6.   Table 6.
The present work confirms the medicinal value of plant extracts over the available commercial antibiotics against microbial pathogens reported in co-infection, especially for MDR pathogens. The study strongly correlates co-infections with a bacterial and fungal origin, which can be the best possible niche for viral superinfections in the context with COVID-19. The possible treatments against these superinfections at times are not effective due to the MDR co-infections, making it almost untreatable. Hence, the strategy in fighting co-infections by the use of natural products with almost the least toxicity status can bring major advancement in treating viral superinfections. The best advantage of the study is that apart from being antimicrobial, the selected natural products have demonstrated their history as having antiviral, antioxidant, anti-inflammatory, as well as immunomodulatory potential. Therefore, the products used in the study define a multifunctional holistic approach with evidence-based results for immediate use in clinical settings for rapid results.

Discussion
According to a cohort study, 20 out of 90 people with severe acute respiratory syndrome reported additional lower respiratory tract infections in 2003, and these infections were responsible for the need of a major invasive operative procedure in 70.6% of critical SARS patients. The pathogens causing secondary infections in SARS patients were diverse. The negative bacilli, including Candida, were the most common of them [6]. Isolates along with selected MDR characteristics have been identified based on culture, morphological, biochemical, and molecular characteristics. If any pathogen shows resistance against three or more antibiotics, it is called a multidrug-resistant pathogen. Bacterial isolates such as Pseudomonas aeruginosa, Escherichia coli, and Moraxella catarrhalis (gram negative) showed MDR characteristics. Transmission of MDR bacteria to the community is a crucial process of triggering infectious disease outbreak and is correlated with elevated morbidity, death, healthcare expenses, and antibiotic usage [34]. Isolates have been tested for antibiotic resistance using different antibiotics that displayed differing degrees of susceptibility and resistance to various antibiotics and antifungals. In many cases, clinicians have misinterpreted fungal pulmonary infection due to a lack of specific clinical symptoms, which causes a high rate of morbidity and mortality. Different studies on pulmonary tuberculosis reported the prevalence of around 15-32% Candida spp. co-infection [35]. Immunocompromised patients with invasive pneumonia reported significant Candida spp. infection [36]. Bacterial and fungal co-infections with marked drug resistance were confirmed after ruling out for tuberculosis (TB) infection in the respiratory tract infected (RTI) patients. This made them the ultimate model for superinfection. According to the Centre for Disease Control (CDC), a superinfection is an infection following a previous infection that is specifically caused by microorganisms that are or have become antibiotic-resistant even before the superinfection, while a co-infection is an infection occurring in tandem with an initial infection [37]. Thus, the difference is clear that co-infections occur simultaneously, whereas superinfections develop following the initial infection [38]. A rapid diagnosis of co-infection is required; this emphasizes the need for methods that are capable of detecting a wide array of potential pathogens with drug-resistant strains as well as subsequent monitoring for infection development. Such a study would provide valuable surveillance data, thereby helping in the antibiotic prescribing policy. Nevertheless, very little importance has been given to secondary bacterial and fungal infections with a scarcity of standardized diagnostic procedures. Likewise, few challenges prevail in diagnosing secondary infection in COVID-19 patients [39]. A rapid characterization of co-infection in the most severe COVID-19 patients is essential in management and treatment, which in turn will also improve antimicrobial stewardship throughout the pandemic, saving more lives than causing deaths [3]. Various pathogenic bacteria present in respiratory tract infections have shown marked resistance against a wide range of commonly used antibiotics. The increase in resistance of gram-negative bacteria (faster than in gram-positive bacteria) is higher than active drug candidates or antimicrobials with a few drug development programs, making an insufficient therapeutic cover for almost the next two decades [40].
In this study, 120 sputum samples were collected, out of which 85 (70.83%) patients showed a positive incidence for different bacterial and fungal pathogens, which included S. aureus, Pseudomonas aeruginosa, and Candida spp., and were also reported by [41]. All samples were non-AFB sputum samples but also showed the presence of other pathogens, which has been already confirmed in a Cambodian study by [42]. Hence, it was concluded from the study that the sputum samples that were negative for AFB also showed the incidence of pathogenic bacterial isolates such as Escherichia coli, Pseudomonas aeruginosa, Moraxella catarrhalis, Staphylococcus aureus, Serratia spp., Enterobacter spp., and Candida spp. as fungal isolates. Data regarding the bacterial or fungal infection associated with viral pneumonia caused by coronavirus are limited. All the isolated pathogens show sensitivity against the selected plant extracts used. Plant-based products have recently demonstrated massive potential in designing therapeutics and diagnostics [43,44]. Herbal intervention for respiratory illness associated with cough provides estimates of the effectiveness of treatments and their associated side effects [45]. Increasing demand for the bioprospection of plants for drugs is steadily escalating, which entails screening medicinal plants with promising biological activity. Medicinal plants are the gift of nature to cure a number of diseases amongst human beings [46]. Three medicinal plants are used in our study, viz. Moringa oleifera, Cassia fistula, and Adhatoda vasica. Moringa oleifera seed oil (MOSO) can be considered for the production of organic surface coating resins. The polyester-amide (MOPEA) of MOSO was successfully synthesized and characterized, and it showed excellent antimicrobial inhibitive properties. The remarkable solubility property of MOPEA in most tested solvents reveals its thermal stability and indicates how promising the resin will become when applied in the surface coating industry [22]. The use of Cassia fistula as standardized formulations in the form of hand sanitizers and soaps has been widely used recently, and it needs further optimization for it to be used as a disinfectant and antiseptic. Apart from that, Cassia fistula seed gum has been used as a film coating material for drugs as well as for a variety of other pharmaceutical and bioengineering coating purposes [18]. The applicability of the Adhatoda vasica methanol extract in the preparation of medicinal liquid-based soaps, which demonstrated substantial antibacterial activity in liquid form compared to the dry extract type, has been well documented in previous studies [24]. The extract of the Adhatoda vasica effectively prevented the corrosion of a steel surface in a 0.5 M H 2 SO 4 solution. The anticorrosion activity of the Adhatoda vasica extract increased significantly on increasing the concentration of extract and decreased with a rise in temperature while the potentiodynamic polarization measurement study confirmed its mixed-type inhibition activity [25].
Pathogenic isolates are tested against acetone and methanol extracts from selected medicinal plants, which showed a broad range of antimicrobial activity, marking a 100% sensitivity against the isolated pathogens including MDR isolates. Acetone and the methanol extract of Moringa oleifera showed the highest zone of inhibition (17.3 ± 0.3, 17.6 ± 0.6) against MDR isolates. M. oleifera extracts from seeds, roots, leaves, and a mixture in ethyl acetate, acetone, and ethanol as solvents were respectively assessed for antibacterial and antifungal potential on infected dental patients [47]. However, Rani et al. exhibited the Moringa extract to be a stronger inhibitor against gram-positive species than gram-negative species. The compound 4-[(-l-rhamnosyloxy)-benzyl] isothiocyanate is responsible for the antimicrobial activity of M. oleifera against Staphylococcus aureus, Staphylococcus epidermis, Bacillus subtilis, Epidermophyton floccosum, and Trichophyton rubrum, as shown by [19]. Acetone extract of Cassia fistula gives the highest zone of inhibition (18.6 ± 0.3) against S. aureus. Different parts of C. fistula are used as an important source of naturally occurring bioactive compounds and show activity against a wide range of pathogenic microorganisms [48]. However, the acetone extract of Adhatoda vasica gives the highest zone of inhibition (17.0 ± 0.0) against Pseudomonas aeruginosa. A. vasica has been used indigenously by natives as folkloric medicine for ages, especially in the treatment of respiratory disorders such as coughs, colds, asthma, etc. [23]. Bag et al. showed that different parts of Adhatoda vasica are used against a wide range of chronic infections, amongst which the leaves show potential activity in COVID-19 patients as shown by [49], consequently manifesting strong evidence about plant extracts as more effective than the available antibiotics by showing their full sensitivity against respiratory tract infected microbial pathogens. Herbal drugs present more effective and safer options for upper respiratory tract infection (URTI) patients and are recognized as key materials for future research [45]. A recent increase in antibiotic resistance amongst major pathogenic groups including respiratory tract infections (RTI) has made alarming concerns in the diagnosis and treatment with the existing therapeutics. Hence, alternative treatment strategies and effective prognosis of RTI is the urgent need of the hour. Significant immunomodulatory and anti-inflammatory activities by plants used in our study also have been reported, which increase in manifolds their efficacy in treating patients with multifactorial disorders, thereby proving their multifunctional potential [50]. The medicinal plants used in the study has tremendous potential to be used by industry experts in various forms, such as toothpaste, mouth wash, water purifying agents, tablet coatings, tissue-engineered wound dressing materials, as well as in the form of surface coatings of steel and other biomedical engineered devices and nanotechnological based bioproducts [19].
As per the Indian system of medicine, the present protocol by the Ministry of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy (AYUSH) in India has recommended the use of plants such as Moringa oleifera and Adhatoda vasica for administration to patients with mild infection of COVID-19 who are in quarantine [13]. As per our study, we also suggest and recommend the use of a magical trio of Moringa oleifera (antiviral) and Adhatoda vasica (antibacterial) along with Cassia fistula (antifungal) in asymptomatic as well as symptomatic patients along with patients having any secondary or respiratory co-infections. Accordingly, our study will provide a scientific evidence-based proactive strategy in treating COVID-19 cases in the future, thereby supplementing the present AYUSH protocol with cheaper and effective dosages easily available within a given treatment regimen. In the context with the recent Covid-19 pandemic, more multidisciplinary approaches such as artificial intelligence [51,52] and nanotechnology-enabled approaches could be front-runners to fight against the transmission as well as advocate the prevention and therapeutics of such cases. Further, we have to understand the recently emerging complex cellular physiology of the bacteria [53,54] and infectious viruses to implement better preventive foresight.

Conclusions
From our study, it can be concluded that traditional herbal remedies from the Indian system of medicine, i.e., Ayurveda, can be implemented to treat co-infections in this ongoing pandemic. This research connected evidence-based data to co-infections in respiratory tract infections and their role as potential predisposing factors in the COVID-19 infection and potential medication strategies. This study very specifically proves the multifunctional potential of selected medicinal plants as antimicrobials in treating co-infections, including the MDR strains with the least toxicity status. The study provides a paradigm shift by providing scientific evidence to help in noninterventional treatment at low cost and easy availability as these medicinal plants have a long history where potential bioactive compounds were provided from them, which are in use for decades and are marketed as active pharmaceutical ingredients. Since the plants used in this study have well documented antimicrobial potential, advanced therapeutics with increased efficacy can be produced by using a nanotechnological approach. In the future, drug coating and scaffolding using these medicinal plant extracts synergistically can be used significantly for better and effective treatment strategies. We can finally say that Moringa oleifera, Adhatoda vasica, and Cassia fistula can be very effective in managing COVID-19 associated co-infections in asymptomatic as well as predisposed patients with a holistic approach. The data here are preliminary, and further clinical trials on larger sample size are needed to validate these and bring them into action for public use.  Figure S2: Isolated microorganisms in Nutrient agar media, Figure S3: (A-G) Antibiotics and antifungal susceptibility pattern of isolates.
Author Contributions: Conceived and designed the experiments, A.T.P., I.P., A.V.S., and M.P.S.; performed the experiments, A.T.P.; analyzed the data: A.T.P., I.P., and A.K.; contributed to reagents/materials/analysis tools: P.Z., D.G., A.V.S., and M.P.S.; wrote the paper: A.T.P. and I.P.; directed the research: M.P.S. and A.V.S. All authors have read and agreed to the published version of the manuscript.
Funding: This research received no external funding.