Development of Polylactic Acid Films with Selenium Microparticles and Its Application for Food Packaging

Selenium is a natural element which exists in the human body and plays an important role in metabolism. Along with this, selenium also possesses antibacterial and antioxidant properties. Using selenium microparticles (SeMPs) in food packaging films is exceptional. In this experiment, a solution casting method was used to make film. For this purpose, we used polylactic acid (PLA) as a substrate for the formation of a film membrane while SeMPs were added with certain ratios to attain antibacterial and antioxidant properties. The effects of SeMPs on the PLA film and the value of SeMPs in food packaging film production were investigated. The effects of the SeMPs contents on the features of the film, such as its mechanical property, solubility, swelling capacity, water vapor permeability, antioxidant activity, and the antibacterial activity of the composite membrane against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative) strains, were studied. The results manifest that the PLA/SeMPs films showed higher water resistance, UV resistance, antioxidant activity, and antibacterial activity than pure PLA film. When the concentration of SeMPs was 1.5 wt%, the composite membrane showed the best comprehensive performance. Although the tensile strength and elongation at break of the membrane were slightly reduced by the addition of SeMPs, the results show that PLA/SeMPs films are still suitable for food packaging and would be a very promising material for food packaging.


Introduction
In recent years, due to environmental protection and the sustainability of renewable resources, the demand for biodegradable packaging is rising [1,2]. Plastics are multifaceted materials that are extensively used in food packaging as well as in non-food items. Polylactic acid (PLA) is a new material derived from corn, sugar beets, potato starch or other plants that can regenerate [3,4]. Many studies have shown that it is biodegradable into CO 2 , H 2 O and other small molecules in composting conditions [5]. In addition, it also has many excellent properties, such as strong applicability, low cost, good biocompatibility, optical clarity, biodegradability, good processing properties, thermoplasticity and excellent mechanical properties [6,7]. Due to these excellent performances, PLA is not only used in packaging but also used in various products like cups, bathtubs, packaging films, pallets and other products [8,9]. The Food and Drug Administration (FDA) also recognizes PLA as GRAS (generally recognized as safe), such that it can encounter food [10]. It is one of the most promising polymeric materials with high application value [11]. added to 20 g of chloroform at room temperature and kept under vigorous stirring with a stirrer until the PLA was completely dissolved, and thus a 10% (w/w) PLA solution was prepared. Span-80 was added to the solution with a mass fraction of 2% as a plasticizer. The solution was then poured onto a clean and flat glass plate (12 × 12 cm 2 ). The glass plate containing the solution was put into the oven and dried at 50 °C to prepare pure PLA membranes. SeMPs (0.1 g; 0.2 g; 0.3 g; 0.4 g) were added up to the mixed solution (10% PLA and 2% Span-80) and stirred intensely for 20 min, after which the PLA/SeMPs membrane solution was obtained. The solutions of different proportions were poured on the glass plate and dried at 50 °C until the solvent completely evaporated to prepare composite membranes (Table 1), which were then stored in dry conditions until use for further testing.

Morphology Studies
The morphological analysis of PLA and PLA/SeMPs composite membranes was done by scanning electron microscopy (SEM; JSM-639OLV, JEOL, Tokyo, Japan) and transmission electron microscopy (TEM; 2100 microscope, JEOL, Tokyo, Japan). For SEM analysis, the sample was coated

Morphology Studies
The morphological analysis of PLA and PLA/SeMPs composite membranes was done by scanning electron microscopy (SEM; JSM-639OLV, JEOL, Tokyo, Japan) and transmission electron microscopy (TEM; 2100 microscope, JEOL, Tokyo, Japan). For SEM analysis, the sample was coated with the gold particles through the physical vapor deposition (PVD) technique. The coating was done by Baltec SCD Coatings 2020, 10, 280 4 of 17 005 sputter coater, using a 30-mA current from 50 mm distance for 180 s. The sample was prepared by placing drops of suspension containing particles on the carbon films which are supported by 300-mesh grid of coper for the transmission electron microscope (TEM) analysis.

Fourier-Transform Infrared Experiment (FTIR)
The attenuated total reflectance Fourier transform infrared (which is denoted by the abbreviation (ATR-FTIR)) spectrometry technique was used to study the chemical structure of the PLA and PLA/SeMPs composite membranes and to observe possible interactions between PLA and SeMPs. The specimens were cut into small sections, each for different composite membranes, and they were analyzed in resolution at 4 cm −1 . The velocity for scanner was 2.2 kHz, the aperture setting was set as 6 mm, the scan time was 32 s for the background, the scan time for the sample was 32 s, the total scans per sample were 100, and the range was 400-4000 cm −1 . The spectral output was recorded in the absorption mode, as a function of the wavenumber, a Bruker 66 spectrometer (Jena, Germany) was used.

Density Test
The flat and smooth composite membranes were cut into samples with area s. The mass of each sample was measured with electronic scales with an accuracy of 0.1 mg, denoted as m. The thickness of the samples was measured with a thickness gauge. We measured the thickness from one center point and four surrounding points of the sample, and then recorded the average thickness d, with an accuracy of 1 µm. The above steps were repeated three times for each membrane sample and the average value was used. The membrane density 'ρ' was computed according to the following formula [39]:

Mechanical Property Analysis
Each film was cut into rectangles 100 mm in length and 10 mm in width. A universal testing machine (UTM, Instron 5583, Norwood, MA, USA) was used for tensile strength tests with crosshead speeds of 250 mm/min and an 80 mm gauge length. Each film was tested five times, and the average value was recorded for subsequent analysis [40].

Swelling Capacity and Solubility of PLA/SeMP Composite Membranes
The swelling capacity and solubility of the PLA and PLA/SeMP composite membranes were tested using the method exploited by Gontard et al. [41]. The membranes were cut into rectangles 40 mm in length and 10 mm in width, and then weighed, denoted as the initial weight m 1 of the film. The rectangular sample was immersed in distilled water (50 mL) for 24 h, and allowed to settle at room temperature. Then, we removed the samples from the water, drained the surface carefully with filter paper and then weighed the samples. We repeated these steps until we obtained a constant weight, which was recorded as m 2 . Then, the samples were placed in an oven at 100 • C to dry, until the weight stabilized, and the weight was recorded as m 3 . Each value was the average of three samples for parallel experiments. The following formula was used for the calculation of swelling capacity and solubility: where m 1 (g) is the initial weight of a rectangular sample, m 2 (g) refers the weight of the rectangular sample after water absorption, and m 3 (g) represents the weight of the rectangular sample after drying.

Permeability Experiments
Referring to the method reported previously by Fabra et al. [42], we first fixed the samples then placed the silica gel in a drying tower (with a temperature of 25 • C, with a relative humidity of 0%). The control sample was a cup covered with aluminum foil to estimate the solvent loss during the sealing. Once the stable state was obtained, the analytical balance (accuracy 0.0001 g) was used for weighing the cup every 2 h. The slope of steady-state permeability with time in the weight loss curve was used to calculate the water vapor permeability coefficient. The mass loss was obtained by subtracting the loss through sealing from the total mass loss. Finally, the thickness of the samples was measured to calculate the water vapor permeability. Each value was the average of three samples for parallel experiments.

Color Measurements
The color parameters of the composite membranes were measured by a CR-400 Chroma-Meter (Konica Minolta, Barcelona, Spain) [43]. A composite membrane was placed on the surface of a white standard plate (calibration plate values: L* = 88.04, a* = 0.72, and b* = −4.34). Color parameter measurement used the CIELAB color scale: L* = 0 (black) to L* = 100 (white), −a* (greenness) to +a* (redness), −b* (blueness) to +b* (yellowness), and the ∆E* (color difference) was calculated as: where the white standard plate was the control sample. Each sample was measured 10 times.

DPPH Free Radical Scavenging Activity
The antioxidant activity of the PLA and PLA/SeMP composite membranes was evaluated by assaying the DPPH free radical scavenging [44], with a slight modification. All composite membranes were cut into 20 mm × 20 mm size samples, which were placed in a beaker filled with 50 mL of absolute ethyl alcohol, and then mixed until complete dissolution. The DPPH solution was prepared by mixing 1 mL of membrane solution with 4 mL of absolute ethyl alcohol of DPPH (75 × 10 −3 mol/L). The DPPH solution was kept in the dark for 30 min at room temperature. The UV absorbance of the determination of DPPH solution was determined at 517 nm. The following formula was used to calculate the DPPH scavenging activity: where A DPPH is the UV absorbance of the absolute ethyl alcohol solution of DPPH at 517 nm and A S is the UV absorbance of the determination of DPPH solution at 517 nm. Detections were tested three times for each sample, and the average values were considered.

Light Transmittance
The composite membranes with smooth surface and no mechanical damage were selected to measure the light transmittance. Measurements were performed using the method developed by Yu et al. [45]. The composite membrane was cut into rectangles 10 mm in width and 40 mm in length. Then, the rectangular specimens were placed tightly on the inner wall of a 10-mm colorimetric ware for light transmittance measurements (600 nm using a UV-visible spectrophotometer). Blank colorimetric ware was used as a control. Each value used for analysis was the average of three parallel experiments.

Seal strength Determination
Referring to ASTM Standard Method F88 for seal strength measurement of the composite membranes with slight modifications, the composite membranes were cut into strip samples 100 mm in length and 15 mm in width. A 10 mm heat-sealed area was sealed at 130 • C and 450 KPa for 3 s. The 2 unsealed edges of the sealed membranes were clamped separately to the universal testing machine (UTM, Instron 5583, Norwood, MA, USA) and held perpendicular to the test direction. A crosshead speed of 300 mm/min and a gauge length of 80 mm were used. Seal strength is the maximum force required to cause seal failure, expressed in N/m:

Antimicrobial Properties
The antimicrobial properties of the membranes were determined using the technique provided by Otoni et al. [46] with some modifications. E. coli and S. aureus (0.2 mL 10 5 CFU/mL) were inoculated in nutrient agar medium, respectively, and cultured for 24 h. Then, small disks 12 mm in diameter were cut from the samples (PLA and PLA/SeMP composite membrane, respectively) and were placed on the corresponding medium, cultured at 37 • C for 24 h. The inhibition zones were measured with a caliper, recorded in mm. All tests were performed in triplicate.

Statistical Analysis
All experiments tested multiple samples, and the final value was expressed as the mean ± standard deviation. SPSS software was used to conduct one-way analysis of variance (ANOVA). A value of p < 0.05 was considered significant.  Figure 2b shows the SEM micrographs of the corresponding films. The surfaces of the PLA present a continuous microstructure, which smooth and homogeneous, without holes or cracks. The PLA/SeMP composite membranes have no cracks, air bubbles, droplets and pores. Changes in the morphology of the PLA/SeMP membranes were significant compared to those noted for the PLA membranes. The surface of pure PLA was smooth and dense, while the surface of the PLA/SeMP membranes showed a slightly rough texture and the nanoparticles were distributed in the PLA matrix. The surface roughness arose from the SeMP molecules disrupting the compact structure of the PLA matrix. As the concentration of SeMP increased, the surface roughness of the membranes increased. As the SeMPs were dispersed in the chloroform solution for PLA dissolution without any surface treatment, the SeMPs appeared on the surface of the membrane. When the dosage was less than 1.5%, the distribution of nanoparticles was more uniform. Particle agglomeration occurred when the dosage of nanoparticles was increased further. Similar events have been reported in the previous literature [47]. To determine the morphology of SeMPs in the polymer, TEM was performed, and the microstructure images of the PLA/2% SeMP films are presented in Figure 2c. As seen in the figure, SeMPs were randomly distributed in the PLA matrix. At this concentration, the SeMPs began to agglomerate, corresponding to our intuitive assumption.

Morphology and Surface Characteristics of PLA/SeMPs Films
distribution of nanoparticles was more uniform. Particle agglomeration occurred when the dosage of nanoparticles was increased further. Similar events have been reported in the previous literature [47].
To determine the morphology of SeMPs in the polymer, TEM was performed, and the microstructure images of the PLA/2% SeMP films are presented in Figure 2c. As seen in the figure, SeMPs were randomly distributed in the PLA matrix. At this concentration, the SeMPs began to agglomerate, corresponding to our intuitive assumption.

Infrared Spectra
FTIR spectrum is an effective technique to research the interaction between functional groups based on the vibrational band shifts. Further characterization of the PLA and PLA/SeMP composite membranes was done by applying FTIR spectra. The band of PLA at 2923 cm −1 represents the asymmetric stretching vibration of CH; the band at 1747 cm −1 represents the stretching vibration of C=O; the band at 1455 cm −1 is the bending vibration in the s-CH(CH 3 ) plane; the peak value at 1081 cm −1 stands for C-O stretching vibration and the peak values at 870 cm −1 and 753 cm −1 represent C-C stretching vibrations. Based on analysis of the spectra obtained for PLA, PLA/SeMPs, and SeMPs (Figure 3), the FTIR spectra of the membranes with or without nanoparticles showed similar general characteristics. There was no indication of chemical interactions between PLA and the SeMPs. No supplemental peak formation was observed, suggesting that no chemical bonds were formed between the PLA and SeMPs. This suggests that SeMPs were physically trapped within the polymer matrix, in agreement with the previously discussed results [48,49].

Infrared Spectra
FTIR spectrum is an effective technique to research the interaction between functional groups based on the vibrational band shifts. Further characterization of the PLA and PLA/SeMP composite membranes was done by applying FTIR spectra. The band of PLA at 2923 cm −1 represents the asymmetric stretching vibration of CH; the band at 1747 cm −1 represents the stretching vibration of C=O; the band at 1455 cm −1 is the bending vibration in the s-CH(CH3) plane; the peak value at 1081 cm −1 stands for C-O stretching vibration and the peak values at 870 cm −1 and 753 cm −1 represent C-C stretching vibrations. Based on analysis of the spectra obtained for PLA, PLA/SeMPs, and SeMPs (Figure 3), the FTIR spectra of the membranes with or without nanoparticles showed similar general characteristics. There was no indication of chemical interactions between PLA and the SeMPs. No supplemental peak formation was observed, suggesting that no chemical bonds were formed between the PLA and SeMPs. This suggests that SeMPs were physically trapped within the polymer matrix, in agreement with the previously discussed results [48,49].  Table 2 lists the densities of the PLA and PLA/SeMP composite membranes. The density of the   Table 2 lists the densities of the PLA and PLA/SeMP composite membranes. The density of the PLA was higher than all the composite membranes, which was 1.14 ± 0.09 g/cm 3 . Therefore, we can consider that the addition of SeMPs reduces the density of the PLA matrix. This may be due to the interactions between the two constituent materials and the violent agitation destroyed the original dense structure of the PLA matrix [39]. Moreover, on further addition of SeMPs, a trend of increase followed by a decrease was noted. The density increase may have been due to the increase in the solid content associated with SeMPs and the uniform distribution of SeMPs among PLA molecules [50]. When the concentration of SeMPs was so high that agglomeration occurred, the density decreased again [51]. When the mass fraction of SeMPs was 1.5%, the density reached the highest value for the composite membrane, at 1.10 ± 0.04 g/cm 3 -only 3.51% lower than that of the PLA film. As the mass score of SeMPs continues to increase to 2%, the density of the composite membrane suddenly decreased to a minimum of (0.91 ± 0.02) g/cm 3 , which is 20.18% lower than that of the pure PLA.

Tensile Properties
The membrane, which is used to keep food fresh, needs to remain intact under external pressure: therefore, excellent mechanical properties are essential [52]. Furthermore, elongation at break (%E) and tensile strength (TS) are major features in mechanical properties [53,54]. Table 2 shows the effects of adding different concentrations of SeMPs on the mechanical properties of the PLA membrane; the addition of SeMPs reduced the overall %E and TS for the membranes.
The TS of the composite film tended to worsen with an increase in the SeMPs content. When the mass fraction of SeMPs was 0.5%, the TS was 13.45 ± 1.13 MPa. When the mass fraction of SeMPs was 2%, the TS of the film was the lowest, having decreased by 70.48% compared to that of the PLA membrane. The decrease in TS could be due to the high-speed magnetic agitation that caused some of the SeMPs to enter the PLA macromolecule chain during the film formation process in the composite film solution [55,56]. This damaged the original structure of the PLA, resulting in an increase in the brittleness and decrease in the binding force [57].
The lowering of %E on addition of additives and/or a filler to polymers is also a common trend observed in thermoplastic composites [58]. In this case, the %E of the PLA/SeMP composite film showed a tendency of first improvement and then deterioration. The trend noted for the %E might mainly be attributable to the particle shape [59]. When the mass fraction of SeMPs was 1%, the best %E of 181.70% ± 20.51% was noted. Then, the performance began to deteriorate, which might have been due to the aggregation of SeMPs [51].
Although the addition of SeMPs resulted in an overall decrease in the mechanical properties of the membrane, it still meets the requirements of food safety packaging [56]. The composite membrane with SeMPs content of 1% showed the best mechanical properties: its TS and %E were 11.45 ± 0.84 MPa and 181.70% ± 20.51%, respectively.

Swelling Capacity and Solubility
The swelling capacity and solubility are very important factors for membranes, affecting their water resistance, especially in humid environments [49]. As shown in Figure 4, the PLA membranes were characterized by a higher swelling capacity than that of the PLA/SeMPs composite membranes. The addition of SeMPs significantly (p < 0.05) reduced the swelling degree of the membrane in water. This may be because the SeMPs molecules entered the PLA molecules and blocked many gaps between the PLA molecules, so that water molecules could not enter these gaps and be adsorbed [60]. When the mass fraction of SeMPs was 2%, the swelling rate of the composite film was 0.15 ± 0.01%, which was the lowest, and 94.81% lower than that of PLA membrane.

Water Vapor Permeability
The water vapor permeability (WVP) reflects the ability of a film to prevent moisture transfer [61]. It is one of the important indices that help to evaluate the functions of edible membranes, which is influenced by numerous factors, such as the relative proportions of the ingredients in the formula, the thickness of the membrane, humidity and water activity [62]. A difference in the WVP of nanoparticle-containing films may be due to various factors, such as compatibility between the polymer matrix and the nanoparticles, the filler concentration, and the type of polymer used [63].
The WVP of the PLA and composite membranes is shown in Figure 5. The WVP of the pure PLA was 5.12 × 10 −14 g·cm/cm 2 ·s·Pa. The WVP first decreased at low SeMP concentrations (below 1.5%) and then increased with further addition of SeMPs. The WVP of the pure PLA was always higher than that of PLA/SeMP composite membranes. The PLA/1.5%-SeMP composite membrane showed the lowest WVP of 3.55 × 10 −14 g·cm/cm 2 ·s·Pa, which is 30.6% lower than that of the pure PLA. This may be due to the SeMPs entering the pores of the PLA molecular chain, resulting in the formation of a tortuous diffusion channel for the water vapor molecules in the polymer matrix [64]. This is like results obtained previously. Shankar et al. [65] reported that when AgNPs were mixed with PLA to prepare the composite membranes, the WVP value of the PLA/AgNPs membrane decreased.
When the mass fraction of SeMPs was 2% (higher concentrations), the WVP of the composite membrane increased: it reached a value of 4.13 × 10 −14 g·cm/cm 2 ·s·Pa, which is only 19.34% lower than that of the pure PLA. It is likely because the nanofillers at high concentrations formed an agglomerated structure without homogeneous dispersion in the polymer matrix [2,66]. No significant difference (p > 0.05) was noted in the solubility of the composite membrane and pure PLA membrane with a change in the SeMP concentration. Since the overall swelling capacity and solubility of the membrane are very low, the material can be considered to protect the food in a humid environment.

Water Vapor Permeability
The water vapor permeability (WVP) reflects the ability of a film to prevent moisture transfer [61]. It is one of the important indices that help to evaluate the functions of edible membranes, which is influenced by numerous factors, such as the relative proportions of the ingredients in the formula, the thickness of the membrane, humidity and water activity [62]. A difference in the WVP of nanoparticle-containing films may be due to various factors, such as compatibility between the polymer matrix and the nanoparticles, the filler concentration, and the type of polymer used [63].
The WVP of the PLA and composite membranes is shown in Figure 5. The WVP of the pure PLA was 5.12 × 10 −14 g·cm/cm 2 ·s·Pa. The WVP first decreased at low SeMP concentrations (below 1.5%) and then increased with further addition of SeMPs. The WVP of the pure PLA was always higher than that of PLA/SeMP composite membranes. The PLA/1.5%-SeMP composite membrane showed the lowest WVP of 3.55 × 10 −14 g·cm/cm 2 ·s·Pa, which is 30.6% lower than that of the pure PLA. This may be due to the SeMPs entering the pores of the PLA molecular chain, resulting in the formation of a tortuous diffusion channel for the water vapor molecules in the polymer matrix [64]. This is like results obtained previously. Shankar et al. [65] reported that when AgNPs were mixed with PLA to prepare the composite membranes, the WVP value of the PLA/AgNPs membrane decreased.

Color Measurements
Optical properties such as the color and gloss are related features, because they directly influencing the acceptance of the product by consumers [67,68]. Visually, both the PLA and PLA/SeMP membranes were transparent, although they darkened with the addition of SeMP. As noted in previous experiments, when the SeMP concentration reached 2%, agglomeration occurs, leading to this darkening. The effect of the SeMP concentration on the color of the films is shown in Table 3. As can be seen from the table, the addition of SeMP reduced the membrane lightness (L*) and resulted in membranes with a more bluish and reddish color, which are determined by the values of a* and b*, respectively. These changes lead to an increase in the total color difference (ΔE*), compared to the SeMPs membranes. Similar changes in color parameters have been reported for chitosan-BSSCE films [69].

DPPH Free Radical Scavenging Activity
DPPH free radical scavenging activity is often used to evaluate the antioxidant activity of specific compounds or food items [40]. The higher the DPPH free radical scavenging rate, the greater the antioxidant activity. As shown in Figure 6, a significant increase was noted in the DPPH radical scavenging activity of PLA/SeMP films with SeMP concentrations of 0.5-2% (p < 0.05), indicating that the antioxidant property of the composite membrane was gradually enhanced. The pure PLA film does not show any antioxidant properties: thus, the antioxidant property of the composite membrane arises from SeMPs. When the concentration of SeMPs in the composite membrane was 2%, the When the mass fraction of SeMPs was 2% (higher concentrations), the WVP of the composite membrane increased: it reached a value of 4.13 × 10 −14 g·cm/cm 2 ·s·Pa, which is only 19.34% lower than that of the pure PLA. It is likely because the nanofillers at high concentrations formed an agglomerated structure without homogeneous dispersion in the polymer matrix [2,66].

Color Measurements
Optical properties such as the color and gloss are related features, because they directly influencing the acceptance of the product by consumers [67,68]. Visually, both the PLA and PLA/SeMP membranes were transparent, although they darkened with the addition of SeMP. As noted in previous experiments, when the SeMP concentration reached 2%, agglomeration occurs, leading to this darkening. The effect of the SeMP concentration on the color of the films is shown in Table 3. As can be seen from the table, the addition of SeMP reduced the membrane lightness (L*) and resulted in membranes with a more bluish and reddish color, which are determined by the values of a* and b*, respectively. These changes lead to an increase in the total color difference (∆E*), compared to the SeMPs membranes. Similar changes in color parameters have been reported for chitosan-BSSCE films [69].

DPPH Free Radical Scavenging Activity
DPPH free radical scavenging activity is often used to evaluate the antioxidant activity of specific compounds or food items [40]. The higher the DPPH free radical scavenging rate, the greater the antioxidant activity. As shown in Figure 6, a significant increase was noted in the DPPH radical scavenging activity of PLA/SeMP films with SeMP concentrations of 0.5-2% (p < 0.05), indicating that Scavenging activity(%) Figure 6. DPPH radical scavenging activities of different composite membranes. Each value represents mean ± standard deviation (SD) of triplicates.

Light Transmittance
Foods spoils easily when exposed to ultraviolet light [70]. Prodpran et al. [71] found that lipid oxidation in foods could be prevented by reducing the light transmittance of the membrane. Thus, the ultraviolet light resistance is the key to food packaging membranes.
As presented in Table 4, the pure PLA was transparent, with a high transmittance value at 660 nm (62.51 ± 2.58%) and a lower transmittance value at 280 nm (19.77 ± 0.86%). The PLA/SeMPs films showed remarkably lower UV-vis light transmittance than that of the PLA film. When SeMPs (2%) were mixed with PLA, the light transmittance values of membranes at 280 nm decreased from 19.77% ± 0.86% to less than 5.51% ± 0.19%, which means that the nanocomposite membranes screened about 72.13% more UV light than that screened by pure PLA. The transmittance values at 660 nm decreased down to 57.18% ± 1.38%, 43.68% ± 1.32%, 40.30% ± 0.93%, and 34.97% ± 0.91% for PLA/0.5% SeMP, PLA1% SeMP, PLA/1.5% SeMP, and PLA/2% SeMP nanocomposite films, respectively. This result is consistent with the physical morphology of the membrane. When the mass fraction of SeMPs was 2%, the light transmittance of the composite membrane was the lowest, reduced by 59.22% compared to that of the PLA membrane.
This indicated that the ultraviolet light barrier properties of the PLA/SeMPs membranes were much stronger than those of pure PLA. Moreover, the light transmittance of the composite films was significantly (p < 0.05) affected by the increase in the SeMP concentration: the ultraviolet light barrier properties of the PLA/SeMPs membranes were promoted with increase in the SeMP content. The above results show that the PLA/SeMPs membranes could effectively protect food against ultraviolet light [69]. The nanocomposite films can be expected to be used as a UV screening for food packaging materials.

Light Transmittance
Foods spoils easily when exposed to ultraviolet light [70]. Prodpran et al. [71] found that lipid oxidation in foods could be prevented by reducing the light transmittance of the membrane. Thus, the ultraviolet light resistance is the key to food packaging membranes.
As presented in Table 4, the pure PLA was transparent, with a high transmittance value at 660 nm (62.51 ± 2.58%) and a lower transmittance value at 280 nm (19.77 ± 0.86%). The PLA/SeMPs films showed remarkably lower UV-vis light transmittance than that of the PLA film. When SeMPs (2%) were mixed with PLA, the light transmittance values of membranes at 280 nm decreased from 19.77% ± 0.86% to less than 5.51% ± 0.19%, which means that the nanocomposite membranes screened about 72.13% more UV light than that screened by pure PLA. The transmittance values at 660 nm decreased down to 57.18% ± 1.38%, 43.68% ± 1.32%, 40.30% ± 0.93%, and 34.97% ± 0.91% for PLA/0.5% SeMP, PLA1% SeMP, PLA/1.5% SeMP, and PLA/2% SeMP nanocomposite films, respectively. This result is consistent with the physical morphology of the membrane. When the mass fraction of SeMPs was 2%, the light transmittance of the composite membrane was the lowest, reduced by 59.22% compared to that of the PLA membrane. This indicated that the ultraviolet light barrier properties of the PLA/SeMPs membranes were much stronger than those of pure PLA. Moreover, the light transmittance of the composite films was significantly (p < 0.05) affected by the increase in the SeMP concentration: the ultraviolet light barrier properties of the PLA/SeMPs membranes were promoted with increase in the SeMP content. The above results show that the PLA/SeMPs membranes could effectively protect food against ultraviolet light [69]. The nanocomposite films can be expected to be used as a UV screening for food packaging materials.

Seal Strength Determination
The seal strength is a key index used to evaluate the quality of packaging materials [57]. Due to the addition of SeMPs, the composite film, a smooth surface was formed on one side and a rough surface on the other on the smooth glass plate. After careful observation, it was determined that the side of the pure PLA film close to the glass plate was smoother than the other side, farther away from the glass plate. Figure 7 displays the sealing strengths of the pure PLA and PLA/SeMP composite membranes.
side of the pure PLA film close to the glass plate was smoother than the other side, farther away from the glass plate. Figure 7 displays the sealing strengths of the pure PLA and PLA/SeMP composite membranes.
During the sealing process, the membranes are in the viscous flow state, and the high molecular weight polymer chains interpenetrate under heat and pressure [57]. Figure 7 shows that the seal strength of the rough surface was significantly higher than that of the smooth surface, irrespective of the film composition. This may be due to the uneven surface increasing the specific surface area of the film during melting, making it easier for the polymer chains to interpenetrate and intertwine and thus increasing the film's sealing performance.
Moreover, the sealing strength of the composite film decreased first and then increased with the addition of SeMPs, regardless of whether it was a rough surface or smooth surface. The sealing strength was the lowest when the mass fraction of SeMPs was 1.5%: it reached values of 945.60 ± 18.62 N/m and 561.60 ± 10.05 N/m for the rough surface and smooth surface, respectively, and then began to increase.
The decrease in the sealing strength may be due to the fact that the SeMPs particles were placed in the gap between PLA molecules after addition, thus blocking the interpenetration and intertwining of the high-molecular-weight polymer chains under heat and pressure [40,57]. The re-increase in the sealing strength was due to the agglomeration of SeMPs.
Although the addition of SeMPs resulted in an overall decrease in the sealing strength of the membranes, they still meet the food safety packaging requirements [72,73]. During the sealing process, the membranes are in the viscous flow state, and the high molecular weight polymer chains interpenetrate under heat and pressure [57]. Figure 7 shows that the seal strength of the rough surface was significantly higher than that of the smooth surface, irrespective of the film composition. This may be due to the uneven surface increasing the specific surface area of the film during melting, making it easier for the polymer chains to interpenetrate and intertwine and thus increasing the film's sealing performance.
Moreover, the sealing strength of the composite film decreased first and then increased with the addition of SeMPs, regardless of whether it was a rough surface or smooth surface. The sealing strength was the lowest when the mass fraction of SeMPs was 1.5%: it reached values of 945.60 ± 18.62 N/m and 561.60 ± 10.05 N/m for the rough surface and smooth surface, respectively, and then began to increase.
The decrease in the sealing strength may be due to the fact that the SeMPs particles were placed in the gap between PLA molecules after addition, thus blocking the interpenetration and intertwining of the high-molecular-weight polymer chains under heat and pressure [40,57]. The re-increase in the sealing strength was due to the agglomeration of SeMPs.
Although the addition of SeMPs resulted in an overall decrease in the sealing strength of the membranes, they still meet the food safety packaging requirements [72,73].

Antimicrobial Properties
Although SeMPs are not considered to be powerful antibiotics, an increase in scientific interest in this subject has been noted in the past few years [48]. One of the important reasons for this growing interest is the finding that elemental nano-Se has the lower toxicity compared to Se compounds and that this microelement is often found in our bodies and is important for our health compared to other popular antimicrobial agents, such as Ag. Although the exact mechanism of SeMPs' antibacterial action has not been fully elucidated, a number of possible mechanisms have been proposed. Induction of reactive oxygen species and oxidative stress are the main mechanisms that have been posited. Another possible mechanism leading to antimicrobial activity is attributable to the smaller sizes of SeMPs, which can spread through the bacterial membrane [74]. It could also be that the bactericidal mechanisms of SeMPs in PLA/SeMPs membranes are different.
The antibacterial activity of the prepared composite membranes with different concentrations of SeMPs were determined against S. aureus (Gram-positive) and E. coli (Gram-negative bacteria) via the agar disc diffusion assay ( Table 5). The potential inhibitory effect of PLA membranes (without SeMPs) was used as a control to investigate. As expected, the PLA membrane showed no antimicrobial activity against E. coli and S. aureus. The antibacterial activity of PLA/SeMP membranes showed significant inhibitory effect on E. coli and S. aureus. The antibacterial activities of the membranes were thus revealed to be directly related to the SeMP content. The bacteriostatic halo of the PLA/SeMP membrane was significantly higher for Gram-negative bacteria (E. coli) than for the Gram-positive bacteria (S. aureus), indicating that Gram-negative bacteria (E. coli) were more susceptible to the PLA/SeMP antimicrobial membranes. However, with the increased SeMPs concentration, the inhibition zone diameters of all tested bacterial strains increased significantly. This indicates that the higher the concentration of SeMPs is, the better the antibacterial performance of the composite membrane. In addition, the PLA/2% SeMP membranes showed the largest inhibition zone against E. coli (13.65 ± 0.06 mm). Jamróz et al. [49] prepared binary-blend membranes of furcellaran and gelatin (FUR/GEL) and FUR/GEL composite membranes reinforced with SeMPs by solution casting. The effect of SeMPs on the antimicrobial activity of the composite membrane was also studied and a similar conclusion was obtained.
The results show that the main antibacterial component of PLA/SeMP composite membranes was SeMP, and the antibacterial ability of the PLA/SeMP composite membranes was better toward E. coli than against S. aureus. This is the first study to analyze the antibacterial activity of PLA membranes incorporated with SeMP. However, the release of SeMPs from PVA/SeMP membranes and the antibacterial mechanism of SeMPs in PLA membrane should be investigated in a future study.

Conclusions
A successful preparation of PLA/SeMP packaging materials was done in this study by the film-casting method. The effects of SeMPs on the properties of the PLA membranes were studied. FT-IR analysis showed that SeMPs were physically trapped within the polymer matrix, and no chemical bond formation was found in the PLA and SeMPs. Although the addition of SeMPs reduced the TS and %E for these membranes in the early stages, it could improve the water resistance, ultraviolet resistance, and antibacterial and oxidation resistance of the material. The optimal composition of PVA/1.5% SeMPs had a significant antibacterial ability on E. coli and S. aureus. Overall, it is feasible to use SeMPs to enhance PLA material, the properties of which were proved. As far as the indicators studied, the properties of PLA/SeMP materials have great application potential and value in the field of food packaging, and it possess great properties for the preparation of food packaging using the SeMPs material. As for the practical value of this material in food packaging, further experiments are needed to verify it. For example, continued investigations are required to research the antifungal activities of these membranes and to evaluate the possible interactions between the food products and packaging membranes. The safety associated with the use of metal nanocomposite membrane materials in food packaging has been a matter of continued concern, owing to queries such as whether the metal nanoparticles in the composite membranes will migrate into the food and whether the migration amount will be within a safe range. These questions need to be resolved by subsequent studies. Finally, further experiments are needed to confirm whether the decline in the mechanical properties of the composite membrane is related to the particle size of the SeMPs added.