Probing the Molecular Interactions of Chitosan Films in Acidic Solutions with Di ﬀ erent Salt Ions

: Understanding the interaction mechanisms of chitosan ﬁlms plays a central role in a wide range of its applications, such as bioadhesive, drug delivery, wound healing, tissue engineering, and wastewater treatment for heavy metal ions. Here, we investigated the molecular interactions between chitosan ﬁlms in acidic solutions with di ﬀ erent salt ions using a surface forces apparatus (SFA). The results showed that chitosan can be adsorbed to mica surfaces by electrostatic interaction under acidic conditions. The force measurements demonstrated that the interactions depend on the salt types, concentrations, and contact time. With the addition of 1 mM LaCl 3 and NaCl into the acetic acid (HAc) bu ﬀ er solution, the cohesion between chitosan ﬁlms enhanced by about 45% and 20%, respectively, after a contact time of 60 min. The enhanced cohesion induced by the combination of partly intermolecular complexation formation in a bridge model and conformation adjustment of chitosan under contact time in 1 mM LaCl 3 solution. However, the cohesion reduced rapidly and even disappeared when the salt concentration increased to 10 mM and 100 mM. We proposed that the cross-linked structures of chitosan mainly contribute to the signiﬁcant reduction of chitosan cohesion in LaCl 3 solution. In comparison, the decrease in cohesion capacity in NaCl solution mainly results from the enhanced hydration e ﬀ ect. Our ﬁndings may provide insights into the interaction mechanisms of chitosan ﬁlms under nanoconﬁnement in acidic conditions and suggestions for the development of chitosan-based materials. erent salt concentrations, including 1, 10, and 100 mM. The pH values of all solutions were titrated to 3.2 after solution preparations to exclude the negative inﬂuence of high pH on chitosan interactions. Ultrapure water was obtained from a water puriﬁcation system, with the resistivity of 18.2 M Ω · cm. All the solutions were ﬁltered using the syringe ﬁlters with 0.22- µ m pore size (Whatman, Guangzhou, China) before use.


Introduction
Chitosan is a linear polysaccharide composed of randomly distributed β-(1-4)-linked D-glucosamine and N-acetyl-D-glucosamine units [1]. Generally, chitosan is the deacetylated derivative of chitin, an essential component of fungal cell walls, crustacean shells, and some parasites [2]. The structure and physicochemical properties of chitosan mainly depend on its molecular weight, the degree of deacetylation (DDA), the pH and ionic strength in solution [3]. The solubility of chitosan is strongly related to the protonation degree of amino groups. Chitosan behaves as a weak cationic polyelectrolyte in dilute acidic solutions with most amino groups being protonated. As a natural biopolymer, chitosan possesses excellent biocompatibility and admirable biodegradability with ecological safety, low toxicity with versatile biological activities, and susceptibility to enzymatic degradation [4]. Therefore, chitosan is considered to be a promising functional material in various applications such as drug delivery [5], pharmaceutical industry [6], food science [7], implants [8], bioadhesives [9,10], and biomaterial [11]. Chitosan is particularly advantageous because it contains plenty of reactive sites other than amino groups, such as hydroxyl and acetyl groups. These reactive sites are easily modified and functionalized, and can interact with other molecules (e.g., polymer, However, the molecular mechanisms of the interactions between chitosan films under aqueous conditions with different salt ions are scarce. A detailed molecular-level understanding of the complex interplay between chitosan and salt ions as well as its influences on surface interactions is essential for applications of chitosan and chitosan-based materials. SFA has been extensively used in studying the governing forces of many biological and non-biological systems [41], due to its unique ability to measure simultaneously the interaction force and the local geometry of two interacting surfaces in situ and in real-time. Coupling with our previous work [42], here we investigated the interaction and cohesion forces between two chitosan films using an SFA in acidic conditions (pH = 3.2) with salt-free, 1, 10, and 100 mM LaCl 3 and NaCl. The results showed obvious cohesion enhancement firstly with the addition of 1 mM salt ions in acidic solutions, whereas the strength of cohesion reduced gradually and finally disappeared when the salt concentrations increased to 10 and 100 mM. This work provides a better insight into the applications of chitosan biopolymer both in biomedical applications and the development of chitosan-based materials.

Materials and Methods
Chemicals. All chemicals were used without further purification. Highest grade (Grade 1) muscovite mica was purchased from S&J Trading Inc. (New York City, NY, USA). Chitosan (product number 448869) with a molecular weight about 50-190 kDa was purchased from Sigma-Aldrich (Shanghai, China), the structure is illustrated in Figure 1. The DDA of chitosan was more than 75.0%. The glassware was washed in advance with sulfuric acid to remove any organic contaminant. Chitosan solution (10 µg/mL) was prepared by dissolving chitosan solute in 100 mM acetic acid (HAc) buffer (Sigma-Aldrich). The solution was then gently stirred overnight at room temperature. NaCl and LaCl 3 (99.9%, Sigma-Aldrich) were dissolved in 100 mM HAc buffer to obtain buffer solutions with different salt concentrations, including 1, 10, and 100 mM. The pH values of all solutions were titrated to 3.2 after solution preparations to exclude the negative influence of high pH on chitosan interactions. Ultrapure water was obtained from a water purification system, with the resistivity of 18.2 MΩ·cm. All the solutions were filtered using the syringe filters with 0.22-µm pore size (Whatman, Guangzhou, China) before use.
Coatings 2020, 10, x FOR PEER REVIEW 3 of 14 essential for applications of chitosan and chitosan-based materials. SFA has been extensively used in studying the governing forces of many biological and non-biological systems [41], due to its unique ability to measure simultaneously the interaction force and the local geometry of two interacting surfaces in situ and in real-time. Coupling with our previous work [42], here we investigated the interaction and cohesion forces between two chitosan films using an SFA in acidic conditions (pH = 3.2) with salt-free, 1, 10, and 100 mM LaCl3 and NaCl. The results showed obvious cohesion enhancement firstly with the addition of 1 mM salt ions in acidic solutions, whereas the strength of cohesion reduced gradually and finally disappeared when the salt concentrations increased to 10 and 100 mM. This work provides a better insight into the applications of chitosan biopolymer both in biomedical applications and the development of chitosan-based materials.

Materials and Methods
Chemicals. All chemicals were used without further purification. Highest grade (Grade 1) muscovite mica was purchased from S&J Trading Inc. (New York City, NY, USA). Chitosan (product number 448869) with a molecular weight about 50-190 kDa was purchased from Sigma-Aldrich (Shanghai, China), the structure is illustrated in Figure 1. The DDA of chitosan was more than 75.0%. The glassware was washed in advance with sulfuric acid to remove any organic contaminant. Chitosan solution (10 μg/mL) was prepared by dissolving chitosan solute in 100 mM acetic acid (HAc) buffer (Sigma-Aldrich). The solution was then gently stirred overnight at room temperature. NaCl and LaCl3 (99.9%, Sigma-Aldrich) were dissolved in 100 mM HAc buffer to obtain buffer solutions with different salt concentrations, including 1, 10, and 100 mM. The pH values of all solutions were titrated to 3.2 after solution preparations to exclude the negative influence of high pH on chitosan interactions. Ultrapure water was obtained from a water purification system, with the resistivity of 18.2 MΩ·cm. All the solutions were filtered using the syringe filters with 0.22-μm pore size (Whatman, Guangzhou, China) before use. Sample preparation. Atomically smooth mica sheets with a thickness from 2 to 5 μm were coated with a 55-nm-thick silver layer. Two back-silvered mica surfaces were then glued on cylindrical silica disks using EPON1004F (Shell Chemicals, Shanghai, China). Chitosan was incubated by dipping glued mica surfaces into 10 μg/mL chitosan HAc buffer solution for 20 min. Then, the surfaces were washed extensively with 100 mM HAc buffer to remove those free or loosely adsorbed chitosan molecules. A droplet of HAc was left on each surface at the end of surface preparation. The whole procedure was performed in a laminar flow hood.
Interaction forces measurements. Interactions between two chitosan-coated mica surfaces across different aqueous buffer solutions were directly measured by an SFA-2000 system from SURFORCE, LLC at Santa Barbara (CA, USA), which has been previously described [43][44][45]. The schematic of the experimental setup in this work was shown in Figure 1. A pair of chitosan-coated mica surfaces were transferred into the SFA chamber in a crossed-cylinder geometry, which corresponds to a sphere of radius R approaching a flat surface. Based on the Derjaguin approximation, the interaction energy per unit area between two flat surfaces (E(D)) can be obtained Sample preparation. Atomically smooth mica sheets with a thickness from 2 to 5 µm were coated with a 55-nm-thick silver layer. Two back-silvered mica surfaces were then glued on cylindrical silica disks using EPON1004F (Shell Chemicals, Shanghai, China). Chitosan was incubated by dipping glued mica surfaces into 10 µg/mL chitosan HAc buffer solution for 20 min. Then, the surfaces were washed extensively with 100 mM HAc buffer to remove those free or loosely adsorbed chitosan molecules. A droplet of HAc was left on each surface at the end of surface preparation. The whole procedure was performed in a laminar flow hood.
Interaction forces measurements. Interactions between two chitosan-coated mica surfaces across different aqueous buffer solutions were directly measured by an SFA-2000 system from SURFORCE, LLC at Santa Barbara (CA, USA), which has been previously described [43][44][45]. The schematic of the Coatings 2020, 10, 1052 4 of 14 experimental setup in this work was shown in Figure 1. A pair of chitosan-coated mica surfaces were transferred into the SFA chamber in a crossed-cylinder geometry, which corresponds to a sphere of radius R approaching a flat surface. Based on the Derjaguin approximation, the interaction energy per unit area between two flat surfaces (E(D)) can be obtained by E(D) = F(D)/2πR, where F(D) is the force between the two curved surfaces, R is the radius of the cylindrical silica disk (usually ∼2 cm), and D is the surface separation distance between two bare mica surfaces. The lower surface was connected to a motor, which allows the two surfaces to move relative to each other at a controlled speed (∼2 nm/s in this study). During the experiments, a droplet of 100 mM HAc buffer (pH = 3.2) was injected into the gap of surfaces when the chitosan-coated mica surfaces were mounted into the SFA chamber. The chamber was sealed and the whole system remained until equilibrium was reached, at which point the force profiles were measured. The two chitosan-coated mica surfaces were approached to contact firstly, external compression remained on them for at least 2 min to reach a closer contact, followed by leaving the surfaces unperturbed for a contact time of 1, 5, 30, and 60 min. Finally, the surfaces were driven apart to investigate the effect of contact time on cohesion forces of the chitosan films. Interaction (cohesion) energy E ad between two surfaces can be determined from the pull-off force (F pull ) taken at the jump-out position by E ad = 2F pull /3πR, which is described by the Johnson−Kendall−Roberts (JKR) model for soft materials with large deformations. The force measurements were also performed in 1, 10, and 100 mM LaCl 3 or NaCl (pH = 3.2) buffer solutions, respectively, to study the influence of salts and concentration on surface interactions. In these measurements, F(D) profiles were measured three times at each condition to confirm the reproducibility. A calibration procedure was performed before each experiment to determine the distance of zero (D = 0) between two bare mica surfaces. The forces F(D)/R were measured with an accuracy of about 10 nN/m and distances with an accuracy of ±0.1 nm. All the measurements were conducted at room temperature of 22 • C.

Interactions between Chitosan Films in HAc Buffer Solutions
Before the tests with the effect of salt ions, the interactions between two chitosan-coated mica surfaces in salt-free HAc buffer solutions were obtained firstly as control references. The surface interactions between chitosan-coated mica surfaces in 100 mM salt-free HAc buffer, at pH 3.2, was shown in Figure 2a. The force profiles exhibit a long-range and exponentially decay electrostatic double-layer repulsion, which was observed at~30 nm separation. Steric repulsion emerges at a separation of D s ≈ 3.6 ± 0.4 nm, indicating the beginning of the interpenetration of adsorbed chitosan molecules. At pH 3.2, the electrostatic repulsion between protonated amino groups prevents the chitosan chains from aggregating, the chitosan exhibits a flattened structure intimately adsorbed on a mica surface. When the initially adsorbed chitosan was slightly pressed, the hardwall thickness (D h ), which represents the thickness of the chitosan films confined between two micas surfaces, reduced to ∼2.5 ± 0.3 nm as the load reaching~10 mN/m. Here, the D h of ∼2.5 ± 0.3 nm indicated that a chitosan film of thickness 1.1-1.4 nm was deposited on each mica substrate in pure HAc solutions. The thickness of adsorbed chitosan films is consistent with other reports [14] and thicker than that on the silica surface [20]. The D h reduced to 1.6 ± 0.2 nm at a higher load (about 30 mN/m), corresponding to a 0.7-0.9 nm adsorbed film on each mica surface. It is difficult to obtain detailed configurations of chitosan under nanoconfinement in our experiments, but the D h indicated a flat configuration of chitosan film on mica. By taking HAc buffer as a 1:1 electrolyte solution with about 0.6% of acetic acid molecules being dissociated [46], the Debye length of the double-layer structure at mica surface can be calculated. We also fitted the forces-distance profiles with the DLVO theory (Derjaguin−Landau−Verwey−Overbeek), which summed the electrostatic double-layer forces and van der Waals forces in the additive [44]. The fitting parameters are the Debye length and surface potential for a general estimation. The solid line in Figure 2a represents the prediction of the DLVO model. The calculation results showed a negligible deviation from the measured decay lengths. (about 10 mN/m) in this experiment than that (about 4 mN/m) in the report. With the increase in contact time, the steric hardwall decreases from ~2.5 ± 0.3 to ~1.6 ± 0.2 nm gradually. That is, the chitosan films adjust their conformation under the prolonged contact time. Increasing contact time also changed the jump-out distance, where the separation between two mica surfaces happened. The jump-out distance points increased from 6.5 ± 0.2 (1 min) to 9.1 ± 0.5 nm (60 min), which is likely due to the enhanced interdigitations and strengthened cohesions with prolonged contact time. It is well known that chitosan contains both hydrophobic methyl groups and hydrogen bond sites, which can contribute to the formation of hydrophobic interaction and hydrogen bonds, respectively [25]. According to previous work [14], chitosan molecules appear as both a relaxed twofold helix and extended twofold helix in acidic solutions. Generally, the relaxed twofold helix structure does not easily form hydrophobic interaction and hydrogen bonds, whereas the extended twofold helix structure could promote the formation of these interactions. The conformation of chitosan molecules may be adjusted as the local surroundings around the chitosan molecules change. As proposed in previous work [14], the contact time effect on chitosan cohesion could be explained by the fact that the chitosan molecule may rearrange itself from a relaxed twofold helix to an extended structure. Additionally, the conformation arrangement of chitosan chains can also be monitored from the decrease in Dh with the increase in contact time. After certain contact time, the Dh was found to decrease from 2.5 ± 0.3 to 1.6 ± 0.2 nm for 60 min. These observations are typical characters of the arrangements of chitosan conformation and formation of a physical interaction bond. The self-adapted conformations can easily form hydrogen bonds and hydrophobic interaction between chitosan-coated surfaces, resulting in stronger cohesion interactions. Furthermore, the decrease in electrostatic and steric repulsion during confinement between chitosan-coated surfaces can increase the cohesion force slightly. Therefore, the conformation change and rearrangement of To assess the contact time effect on the interactions of chitosan films, the pull-off forces F pull between two surfaces during the separation process were measured with different contact times. Two chitosan-coated mica surfaces were firstly brought into contact until the load reached up tõ 10 mN/m, followed by leaving the contacted surfaces unperturbed for different contact times of 1, 5, 30, and 60 min. Then, the surfaces were separated. During the separation process, the lower surface jumps away from the upper surface at certain distances, which was contributed by the cohesion between the two surfaces. The force profiles are presented in Figure 2b. It can be found that the cohesion forces between chitosan films depend strongly on the contact time. The measured cohesion increases by about three times when increasing the contact time from 1 to 60 min. During separation, a strong pull-off force of F pull /R ≈ −14.4 ± 0.9 mN/m was measured between chitosan films with the contact time of 1 min, corresponding to cohesion energy of E ad ≈ −3.0 ± 0.2 mJ/m 2 . By extending the contact time to 5, 30, and 60 min, the pull-off force F pull /R values were enhanced to −22.3 ± 1.1 mN/m (E ad ≈ −4.7 ± 0.2 mJ/m 2 ), −32.8 ± 1.3 mN/m (E ad ≈ −7.0 ± 0.3 mJ/m 2 ), and −42.6 ± 1.8 mN/m (E ad ≈ −9.1 ± 0.4 mJ/m 2 ), respectively. The measured pull-off forces and cohesions are consistent with the previous report [14] except for the contact time of 1 min. This discrepancy may be induced by the higher load (about 10 mN/m) in this experiment than that (about 4 mN/m) in the report. With the increase in contact time, the steric hardwall decreases from~2.5 ± 0.3 to~1.6 ± 0.2 nm gradually. That is, the chitosan films adjust their conformation under the prolonged contact time. Increasing contact time also changed the jump-out distance, where the separation between two mica surfaces happened. The jump-out distance points increased from 6.5 ± 0.2 (1 min) to 9.1 ± 0.5 nm (60 min), which is likely due to the enhanced interdigitations and strengthened cohesions with prolonged contact time.
It is well known that chitosan contains both hydrophobic methyl groups and hydrogen bond sites, which can contribute to the formation of hydrophobic interaction and hydrogen bonds, respectively [25]. According to previous work [14], chitosan molecules appear as both a relaxed twofold helix and extended twofold helix in acidic solutions. Generally, the relaxed twofold helix structure does not easily form hydrophobic interaction and hydrogen bonds, whereas the extended twofold helix structure could promote the formation of these interactions. The conformation of chitosan molecules may be adjusted as the local surroundings around the chitosan molecules change. As proposed in previous work [14], the contact time effect on chitosan cohesion could be explained by the fact that the chitosan molecule may rearrange itself from a relaxed twofold helix to an extended structure. Additionally, the conformation arrangement of chitosan chains can also be monitored from the decrease in D h with the increase in contact time. After certain contact time, the D h was found to decrease from 2.5 ± 0.3 to 1.6 ± 0.2 nm for 60 min. These observations are typical characters of the arrangements of chitosan Coatings 2020, 10, 1052 6 of 14 conformation and formation of a physical interaction bond. The self-adapted conformations can easily form hydrogen bonds and hydrophobic interaction between chitosan-coated surfaces, resulting in stronger cohesion interactions. Furthermore, the decrease in electrostatic and steric repulsion during confinement between chitosan-coated surfaces can increase the cohesion force slightly. Therefore, the conformation change and rearrangement of chitosan molecules are the main reason for the cohesion enhancement between chitosan-coated surfaces with the increase in contact time.

Effects of Salt Ions Types
The conformation and interaction of chitosan may be changed with different types of salt ions. To examine the effect of salt ions on chitosan films, the interactions of chitosan-coated mica surfaces were measured in HAc buffer solutions with different salt ions. Figure 3a shows the typical force-distance profiles between chitosan-coated mica surfaces in HAc buffer solutions with 1 mM LaCl 3 during approach and separation. The other experimental conditions herein are the same as the salt-free case.
Coatings 2020, 10, x FOR PEER REVIEW 6 of 14 chitosan molecules are the main reason for the cohesion enhancement between chitosan-coated surfaces with the increase in contact time.

Effects of Salt Ions Types
The conformation and interaction of chitosan may be changed with different types of salt ions. To examine the effect of salt ions on chitosan films, the interactions of chitosan-coated mica surfaces were measured in HAc buffer solutions with different salt ions. Figure 3a shows the typical force-distance profiles between chitosan-coated mica surfaces in HAc buffer solutions with 1 mM LaCl3 during approach and separation. The other experimental conditions herein are the same as the salt-free case. As shown in Figure 3a, both the surface forces and the Dh vary significantly with the addition of salt ions. Once 1 mM LaCl3 was introduced through buffer exchange, the Dh reduced from ∼2.5 ± 0.3 to ∼1.7 ± 0.2 nm at the steric repulsion of ~10 mN/m during the surfaces approach, indicating that the adsorbed chitosan layer on each mica got thinner by 0.4 nm. The thickness of Dh stabilized at ∼1.2 ± 0.2 nm after the contact time of 60 min, corresponding to about ∼0.6 ± 0.1 nm thickness of chitosan films on each mica surface. Therefore, the conformation of chitosan films became flattened on mica surfaces with the addition of 1 mM LaCl3. The repulsion force was initiated at ~17 nm, which is shorter than that in pure HAc buffer solution. As the addition of 1 mM LaCl3 increased the ionic strength in buffer solutions, the decay length was expected to be shortened and electrostatic repulsion was weakened. The cohesion forces were greatly enlarged with the addition of 1 mM LaCl3 at a given contact time compared to those in salt-free HAc buffer. The puff-off force increased from −14.4 ± 0.9 mN/m (Ead ≈ −3.05 ± 0.2 mJ/m 2 ) in pure HAc buffer to −23.7 ± 0.8 mN/m (Ead ≈ −5.8 ± 0.2 mJ/m 2 ) in 1 mM LaCl3 HAc buffer with only 1-minute contact. By prolonging the contact time to 5, 30 and 60 min, the maximum puff-off forces were enhanced to −28.6 ± 1.0 mN/m (Ead ≈ −7.7 ± 0.2 mJ/m 2 ), −53.9 ± 1.4 mN/m (Ead ≈ −11.4 ± 0.3 mJ/m 2 ) and −61.8 ± 1.8 mN/m (Ead ≈ −13.1 ± 0.3 mJ/m 2 ), respectively. With respect to the film thickness, the Dh under different contact times were measured to slightly fluctuate within the error.
Given that the cohesion between chitosan films was significantly enhanced with the addition of 1 mM LaCl3 in the HAc buffer, it seemed appropriate to examine the effect of type of salt ions on chitosan film interactions. To further clarify the effects of salt ions on the cohesion of chitosan, the force-distance profile between two chitosan-coated mica surfaces was investigated by using the acetic solutions containing 1 mM NaCl, as shown in Figure 3b. In 1 mM NaCl solution, the repulsion between chitosan films is observed at ~19 nm on the approach process. The force range is shorter As shown in Figure 3a, both the surface forces and the D h vary significantly with the addition of salt ions. Once 1 mM LaCl 3 was introduced through buffer exchange, the D h reduced from ∼2.5 ± 0.3 to ∼1.7 ± 0.2 nm at the steric repulsion of~10 mN/m during the surfaces approach, indicating that the adsorbed chitosan layer on each mica got thinner by 0.4 nm. The thickness of D h stabilized at ∼1.2 ± 0.2 nm after the contact time of 60 min, corresponding to about ∼0.6 ± 0.1 nm thickness of chitosan films on each mica surface. Therefore, the conformation of chitosan films became flattened on mica surfaces with the addition of 1 mM LaCl 3 . The repulsion force was initiated at~17 nm, which is shorter than that in pure HAc buffer solution. As the addition of 1 mM LaCl 3 increased the ionic strength in buffer solutions, the decay length was expected to be shortened and electrostatic repulsion was weakened. The cohesion forces were greatly enlarged with the addition of 1 mM LaCl 3 at a given contact time compared to those in salt-free HAc buffer. The puff-off force increased from −14.4 ± 0.9 mN/m (E ad ≈ −3.05 ± 0.2 mJ/m 2 ) in pure HAc buffer to −23.7 ± 0.8 mN/m (E ad ≈ −5.8 ± 0.2 mJ/m 2 ) in 1 mM LaCl 3 HAc buffer with only 1-minute contact. By prolonging the contact time to 5, 30 and 60 min, the maximum puff-off forces were enhanced to −28.6 ± 1.0 mN/m (E ad ≈ −7.7 ± 0.2 mJ/m 2 ), −53.9 ± 1.4 mN/m (E ad ≈ −11.4 ± 0.3 mJ/m 2 ) and −61.8 ± 1.8 mN/m (E ad ≈ −13.1 ± 0.3 mJ/m 2 ), respectively. With respect to the film thickness, the D h under different contact times were measured to slightly fluctuate within the error.
Given that the cohesion between chitosan films was significantly enhanced with the addition of 1 mM LaCl 3 in the HAc buffer, it seemed appropriate to examine the effect of type of salt ions on chitosan film interactions. To further clarify the effects of salt ions on the cohesion of chitosan, the force-distance profile between two chitosan-coated mica surfaces was investigated by using the acetic solutions containing 1 mM NaCl, as shown in Figure 3b. In 1 mM NaCl solution, the repulsion between chitosan films is observed at~19 nm on the approach process. The force range is shorter than that in 100 mM salt-free HAc buffer. With the increase in the contact time, the D h decreased from~1.8 ± 0.2 to 1.3 ± 0.1 nm, which corresponds to an adsorbed chitosan film with a thickness of~0.6 ± 0.1 nm on each mica surface. Slight cohesion capacity decrease was observed after introducing 1 mM NaCl between chitosan films with 1-min contact time, but prolonged contact time (60 min) resulted in a significant enhancement cohesion, which reached F pull /R ≈ −51.3 ± 1.6 mN/m (E ad ≈~10.8 ± 0.5 mJ/m 2 ). Compared with the addition of 1 mM LaCl 3 , the magnitude of cohesion enhancement is smaller.
To make a comparison, a summary of puff-off forces is shown in Figure 4. The cohesion between chitosan films can be enhanced with the addition of 1 mM LaCl 3 and 1 mM NaCl, especially the case with long contact time. Generally, the binding of chitosan molecules to the mica surfaces relies on the protonated amino groups under acidic conditions. With the addition of salt ions, the competition effect of H + makes these binding sites inaccessible for the metal cations in acidic solution. However, as reported in previous work [45], the cation with a high valence can overcompensate the opposite surface charges under some circumstances. The strong ion-ion correlation effect of multivalent ions can result in charge reversion of the mica surface under acidic conditions. It is expectable that the La 3+ cations have a higher affinity with an electronegative nitrogen atom to partly replace the proton in the protonated amino group [47,48]. This exchange process was analogous to ion exchange but at a slow rate due to the electrostatic barriers [42]. The exchanged chitosan molecules can be even more tightly bound with the mica substrate. Thus, the conformation of adsorbed chitosan on the mica surface becomes flatter in 1 mM LaCl 3 solution than that in pure HAc buffer. The intermolecular hydrogen bonds and hydrophobic interactions can be formed easily under flat conformations. Moreover, Cl − anions may also adsorb on the positively charged chitosan molecules as counterions under acidic conditions. Scanning electron microscopy-energy dispersive X-ray spectrometry (SEM-EDX) analysis [49] revealed that the salt cations and anions were simultaneously adsorbed on the chitosan surface from aqueous solution. In this experiment, the existence of Cl − anions in chitosan solution has considerable potential to partly screen the positive charges of chitosan. As a consequence, the net charge of some amino groups is partly neutralized. Under a prolonged contact time, the multivalent salt ions can partly bind with several functional groups from the opposing sides' chitosan chains by intermolecular complexation in a bridge model [31]. Previous works [50,51] reported that the small amount of multivalent ion can enhance the adhesion between catechol protein surfaces by ion bridging. In our case, we speculated that the intermolecular complexation bridge combining with the hydrogen bond and hydrophobic interaction enhanced the cohesion of chitosan films in acidic solutions with the presence of 1 mM LaCl 3 . For the case of 1 mM NaCl, the affinity of Na + ion is lower than La 3+ ion, it is difficult to partly replace the proton in the protonated amino group. However, the ionic strength of the solution was enhanced by the introduction of a small amount of salt ions, which was companied with the compression of electrostatic barrier between the chitosan films from opposing sides. This can promote the adhesive contact as well as conformation adjustment of chitosan molecules chains under prolonged contact time. Therefore, the slight increase in cohesion between chitosan film with the addition of 1 mM NaCl is likely to be related to the electrostatic screening effect in the chitosan network.

Effect of Salt Ions Concentrations
Chitosan can form gels of a complex structure under certain conditions by cross-link agent or multivalent cations, similar to other polysaccharides such as in alginate gels [52]. The binding capacities of chitosan to salt ions were influenced by many factors, such as the DDA, concentration, pH, and salt ions concentrations and types. In order to investigate the effect of concentrations of salt ions on the molecular interactions between chitosan films, the concentrations of LaCl3 and NaCl in 100 mM HAc buffer solutions were increased to 10 and 100 mM, respectively.
As shown in Figure 5a,b, when the concentration of LaCl3 increased from 1 to 10 and 100 mM, the interactions between the chitosan films were obviously different. The decay length of repulsion decreased below 10 nm with the addition of 10 mM LaCl3. The Dh increased to ~3.0 ± 0.3 nm. By extending the contact time to 60 min, the Dh decreased to ~1.4 ± 0.2 nm, which is thicker than those measured in salt-free and 1 mM salt ions buffer solutions. The cohesion was totally eliminated after the contact time of 1 min. Keeping the surfaces at contact for a longer time, the puff-off forces achieve the valves of −8.2 ± 0.8 mN/m (Ead ≈ −1.7 ± 0.2 mJ/m 2 ) for 10 min contact, −22.6 ± 1.1 mN/m (Ead ≈ −4.7 ± 0.2 mJ/m 2 ) for 30 min contact, and −23.7 ± 1.0 mN/m (Ead ≈ −4.8 ± 0.2 mJ/m 2 ) for 60 min contact. The cohesion capacity of the chitosan film was reduced about 55% in acidic solution compared with that measured in 1 mM LaCl3 for the contact time of 60 min.

Effect of Salt Ions Concentrations
Chitosan can form gels of a complex structure under certain conditions by cross-link agent or multivalent cations, similar to other polysaccharides such as in alginate gels [52]. The binding capacities of chitosan to salt ions were influenced by many factors, such as the DDA, concentration, pH, and salt ions concentrations and types. In order to investigate the effect of concentrations of salt ions on the molecular interactions between chitosan films, the concentrations of LaCl 3 and NaCl in 100 mM HAc buffer solutions were increased to 10 and 100 mM, respectively.
As shown in Figure 5a,b, when the concentration of LaCl 3 increased from 1 to 10 and 100 mM, the interactions between the chitosan films were obviously different. The decay length of repulsion decreased below 10 nm with the addition of 10 mM LaCl 3 . The D h increased to~3.0 ± 0.3 nm. By extending the contact time to 60 min, the D h decreased to~1.4 ± 0.2 nm, which is thicker than those measured in salt-free and 1 mM salt ions buffer solutions. The cohesion was totally eliminated after the contact time of 1 min. Keeping the surfaces at contact for a longer time, the puff-off forces achieve the valves of −8.2 ± 0.8 mN/m (E ad ≈ −1.7 ± 0.2 mJ/m 2 ) for 10 min contact, −22.6 ± 1.1 mN/m (E ad ≈ −4.7 ± 0.2 mJ/m 2 ) for 30 min contact, and −23.7 ± 1.0 mN/m (E ad ≈ −4.8 ± 0.2 mJ/m 2 ) for 60 min contact. The cohesion capacity of the chitosan film was reduced about 55% in acidic solution compared with that measured in 1 mM LaCl 3 for the contact time of 60 min. Moreover, as the concentration of LaCl3 was further elevated to 100 mM, the cohesion nearly disappeared. The puff-off force can only reach to −1.9 ± 0.3 mN/m (Ead ≈ −0.4 ± 0.1 mJ/m 2 ) even for 60 min contact. With respect to the film thickness, the Dh increased to ~6.9 ± 0.5 nm, which is larger than that (∼1.7 ± 0.2 nm) in 1 mM LaCl3 solution. The Dh fluctuates with the contact time, decreasing to ~2.8 ± 0.4 nm for 60 min contact. A similar cohesion reduction tendency was observed in 100 mM HAc buffer solutions with the addition of 10 and 100 mM NaCl, except the changing magnitudes, as shown in Figure 5c,d. The repulsion force interaction range becomes much shorter due to the increase in ionic strength. The Dh increased to ~1.8 ± 0.4 nm and ~2.6 ± 0.4 nm in HAc buffer with the addition of 10 and 100 mM NaCl, respectively; with the prolonged contact time, the Dh reduced to ~1.4 ± 0.2 nm and ~1.7 ± 0.3 nm, respectively. At the small separation, the confined chitosan films become more compressed and the repulsive forces are dominated by steric repulsion alone. During separation, we observe obviously decreased cohesion forces with increasing NaCl concentration at a given contact time. The puff-off forces between chitosan films in acidic solutions with the presence of 10 and 100 mM NaCl were −7.3 ± 0.4 (Ead ≈ −1.7 ± 0.1 mJ/m 2 ) and −0.9 ± 0.2 mN/m (Ead ≈ −0.2 ± 0.1 mJ/m 2 ), respectively, for 1-min contact. The cohesion can achieve to −6.1 ± 0.2 and −2.4 ± 0.1 mJ/m 2 for 60 min contact, respectively. The cohesion force in 100 mM NaCl buffer solution almost disappears for the contact time at 1 min.
According to the results from this control experiment, it would take an apparent variance in cohesion between chitosan surfaces with the addition of 10 and 100 mM salts in 100 mM HAc buffer solution. As pointed out above, the enhanced cohesion between chitosan films in 100 mM HAc buffer with the addition of 1 mM LaCl3 was induced by the bridge intermolecular complexation. The formation of multivalent protein-ion complexes can mediate unusual, reversible and strong interactions by regulating the ion concentrations [50,53]. With the addition of high concentration multivalent ions, the multivalent ions can cross-link the chitosan molecules with a concentration of about 10 mM multivalent counterions [12,36]. Polyelectrolytes can even transform from extended brushes to collapsed brushes with saturated trivalent counterions [54]. In this case, more and more La 3+ ions bind to the amino group through ion exchange and are eventually saturated with the increasing concentrations of LaCl3. The thicker Dh by strong compression in HAc buffer with 100 Moreover, as the concentration of LaCl 3 was further elevated to 100 mM, the cohesion nearly disappeared. The puff-off force can only reach to −1.9 ± 0.3 mN/m (E ad ≈ −0.4 ± 0.1 mJ/m 2 ) even for 60 min contact. With respect to the film thickness, the D h increased to~6.9 ± 0.5 nm, which is larger than that (∼1.7 ± 0.2 nm) in 1 mM LaCl 3 solution. The D h fluctuates with the contact time, decreasing to~2.8 ± 0.4 nm for 60 min contact. A similar cohesion reduction tendency was observed in 100 mM HAc buffer solutions with the addition of 10 and 100 mM NaCl, except the changing magnitudes, as shown in Figure 5c,d. The repulsion force interaction range becomes much shorter due to the increase in ionic strength. The D h increased to~1.8 ± 0.4 nm and~2.6 ± 0.4 nm in HAc buffer with the addition of 10 and 100 mM NaCl, respectively; with the prolonged contact time, the D h reduced to~1.4 ± 0.2 nm and~1.7 ± 0.3 nm, respectively. At the small separation, the confined chitosan films become more compressed and the repulsive forces are dominated by steric repulsion alone. During separation, we observe obviously decreased cohesion forces with increasing NaCl concentration at a given contact time. The puff-off forces between chitosan films in acidic solutions with the presence of 10 and 100 mM NaCl were −7.3 ± 0.4 (E ad ≈ −1.7 ± 0.1 mJ/m 2 ) and −0.9 ± 0.2 mN/m (E ad ≈ −0.2 ± 0.1 mJ/m 2 ), respectively, for 1-min contact. The cohesion can achieve to −6.1 ± 0.2 and −2.4 ± 0.1 mJ/m 2 for 60 min contact, respectively. The cohesion force in 100 mM NaCl buffer solution almost disappears for the contact time at 1 min.
According to the results from this control experiment, it would take an apparent variance in cohesion between chitosan surfaces with the addition of 10 and 100 mM salts in 100 mM HAc buffer solution. As pointed out above, the enhanced cohesion between chitosan films in 100 mM HAc buffer with the addition of 1 mM LaCl 3 was induced by the bridge intermolecular complexation. The formation of multivalent protein-ion complexes can mediate unusual, reversible and strong interactions by regulating the ion concentrations [50,53]. With the addition of high concentration multivalent ions, the multivalent ions can cross-link the chitosan molecules with a concentration of about 10 mM multivalent counterions [12,36]. Polyelectrolytes can even transform from extended brushes to collapsed brushes with saturated trivalent counterions [54]. In this case, more and more La 3+ ions bind to the amino group through ion exchange and are eventually saturated with the increasing concentrations of LaCl 3 . The thicker D h by strong compression in HAc buffer with 100 mM LaCl 3 may be viewed as a sign of the cross-linked chitosan molecules. For cross-linked chitosan films, the surface interactions revealed longer range and more repulsive forces, due probably to the network formation resulting in a higher effective modulus of the layers [12]. The obvious force hysteresis existed between chitosan films in HAc buffer with 100 mM LaCl 3 during the compression and decompression process at different contact times, which was absent in pure HAc buffer. Thus, we proposed that the chitosan films can be partly or even totally cross-linked in the highly concentrated salts. The formation of such cross-linked chitosan results in the extraordinary cohesion decrease in the chitosan and the increase in the film's thickness. Meanwhile, the ionic strength of solution was also increased with numbers of extra LaCl 3 salt, making the chitosan films become more accessible for the adsorption of electrolyte ions. Note that La 3+ ion is highly hydrated. More and more hydrated ions with strong hydration shells as well as hydrated Cl − ions may lead to a barrier for the cohesion of chitosan films. The hydration effect on the chitosan films is expected to become stronger with the increase in salt concentrations, which can contribute to the almost vanished cohesion in 100 Mm LaCl 3 . However, the underlying mechanism of cohesion loss may be different for the case of the addition of 10 and 100 mM NaCl. The monovalent Na + ions are more difficult to induce significant cross-linking. The steric hardwall shifts out by about 0.3 nm upon increasing the NaCl concentrations from 10 to 100 mM, implying the permeation of hydrated ions (mainly Cl − ) into the confined chitosan films at a higher salt concentration. The hydrated ions can also bring the disruption of the hydrogen bonds and hydrophobic interactions, which induced the cohesion capacity loss of chitosan films with increasing NaCl concentration at a given contact time. Comparing with the same concentration of LaCl 3 salt ions, the ionic strength of NaCl solution is lower, and either Na + or Cl − possesses weaker hydration energy than La 3+ ions. Thus, it is reasonable that the magnitude of cohesion decrease is larger for LaCl 3 salt than that for same concentration of NaCl salt. By extending the contact time at a given load (about 10 mN/m), the water molecules can be squeezed out from the confined hydrated ions. The decrease in hardwall thickness was an obvious mark. Thus, the cohesion capacity can rescue to some extent by prolonging contact time. Figure 6 shows the contact time evolution of the pull-off forces which reveals important implications for the underlying mechanism of chitosan film interactions. It demonstrates a correlation of cohesion increasing with contact time for each buffer condition. The introduction of salt ions can tune the cohesion between chitosan-adsorbed mica surfaces.

Effect of Contact Time
Coatings 2020, 10, x FOR PEER REVIEW 10 of 14 mM LaCl3 may be viewed as a sign of the cross-linked chitosan molecules. For cross-linked chitosan films, the surface interactions revealed longer range and more repulsive forces, due probably to the network formation resulting in a higher effective modulus of the layers [12]. The obvious force hysteresis existed between chitosan films in HAc buffer with 100 mM LaCl3 during the compression and decompression process at different contact times, which was absent in pure HAc buffer. Thus, we proposed that the chitosan films can be partly or even totally cross-linked in the highly concentrated salts. The formation of such cross-linked chitosan results in the extraordinary cohesion decrease in the chitosan and the increase in the film's thickness. Meanwhile, the ionic strength of solution was also increased with numbers of extra LaCl3 salt, making the chitosan films become more accessible for the adsorption of electrolyte ions. Note that La 3+ ion is highly hydrated. More and more hydrated ions with strong hydration shells as well as hydrated Cl − ions may lead to a barrier for the cohesion of chitosan films. The hydration effect on the chitosan films is expected to become stronger with the increase in salt concentrations, which can contribute to the almost vanished cohesion in 100 Mm LaCl3. However, the underlying mechanism of cohesion loss may be different for the case of the addition of 10 and 100 mM NaCl. The monovalent Na + ions are more difficult to induce significant cross-linking. The steric hardwall shifts out by about 0.3 nm upon increasing the NaCl concentrations from 10 to 100 mM, implying the permeation of hydrated ions (mainly Cl − ) into the confined chitosan films at a higher salt concentration. The hydrated ions can also bring the disruption of the hydrogen bonds and hydrophobic interactions, which induced the cohesion capacity loss of chitosan films with increasing NaCl concentration at a given contact time.
Comparing with the same concentration of LaCl3 salt ions, the ionic strength of NaCl solution is lower, and either Na + or Cl − possesses weaker hydration energy than La 3+ ions. Thus, it is reasonable that the magnitude of cohesion decrease is larger for LaCl3 salt than that for same concentration of NaCl salt. By extending the contact time at a given load (about 10 mN/m), the water molecules can be squeezed out from the confined hydrated ions. The decrease in hardwall thickness was an obvious mark. Thus, the cohesion capacity can rescue to some extent by prolonging contact time. Figure 6 shows the contact time evolution of the pull-off forces which reveals important implications for the underlying mechanism of chitosan film interactions. It demonstrates a correlation of cohesion increasing with contact time for each buffer condition. The introduction of salt ions can tune the cohesion between chitosan-adsorbed mica surfaces. According to the results reported previously [13,14], the increase in contact time allowed structural rearrangements of chitosan molecules and the enhancement of cohesion, due to the formation of hydrogen bonds and hydrophobic interactions of methyl groups by structure changes. According to the results reported previously [13,14], the increase in contact time allowed structural rearrangements of chitosan molecules and the enhancement of cohesion, due to the formation of hydrogen bonds and hydrophobic interactions of methyl groups by structure changes. Similar time-dependent cohesion behavior was observed in another polysaccharide biopolymer [55]. Here, from the film thinning with cohesion increasing as discussed above, we could draw a picture of the contacted chitosan films that undergo a diffusion process at prolonged contact time. When the adsorbed chitosan molecules were exposed to 1 mM LaCl 3 HAc buffer solutions, there were conformation changes in the chitosan films, and a longer relaxation time was required for the formation of effective binding. The prolonged contact time can not only adjust the conformation of chitosan molecules but may also induce the formation of intermolecular complexation in a bridge model, which can enhance the cohesion. When chitosan molecules were exposed to 100 mM LaCl 3 solutions, the chitosan molecules can form cross-linked structures containing many hydrated salt ions. Under this condition, the contact time effect is negligible on its cohesion interactions. Nevertheless, the decrease in the chitosan film thickness with the passing of contact time indicates the adsorbed chitosan films are soft and compressible. The hydrated salt ions absorbed inside the polymer chains can be squeezed out. However, for the case of the high concentration of NaCl buffer solutions, the prolonged contact time mainly squeezes out the hydrated salt ions between the chitosan films that can disturb the formation of the hydrogen bonds and hydrophobic interaction. The results discussing above clearly showed the cohesion capacity recovery by means of extending the contact time. Substantially, the cohesion loss can be considered as the hydrated salt ions combined with the decrease in the number of binding groups. Therefore, this dependence of cohesion between chitosan films on contact time reminds us that chitosan, with enough relaxation time or external compression, can partly rescue its adhesive capacity which has been disrupted by salt ions.

Conclusions
The surface and cohesion forces between chitosan films adsorbed on mica surfaces in HAc buffer with different salt ions were measured using an SFA. The effects of salt concentration and cation valence were also investigated. It is found that chitosan can adsorb on the mica surface with a stable and relatively smooth configuration in HAc buffer at pH~3.2. Extending contact time can promote the molecular conformation changes of chitosan and formation of cohesion bonds (such as hydrogen bonds, hydrophobic interactions), therefore the cohesions were enhanced. The surface cohesion of chitosan films was varied by the introduction of different concentrations and types of salts. The introduction of 1 mM LaCl 3 salts can enhance the cohesion of chitosan films by the combination of partly intermolecular complexation formation in a bridge model and conformation adjustment of chitosan under contact time. However, the introduction of 10 and 100 mM LaCl 3 salt ions confined in chitosan can reduce cohesion interactions by cross-linked chitosan and strong hydration effects. In comparison, the loss of cohesion capacity between chitosan films in 10 and 100 mM NaCl salt solution is mainly induced by the hydration effect. The enhanced hydration effect of highly concentrated salt ions may impede the formation of effective binding between the chitosan from opposing films. Moreover, the bonds mediated by salt ions are not always stable. By applying mechanical compression on the chitosan film, part of the cohesion loss can be rescued after the release of hydrated ion and metal-ion bonds. These investigations provide insights into the interaction mechanism of chitosan molecules under acid conditions with salt ions and similar polyelectrolytes under nanoconfinement and their potential applications.

Conflicts of Interest:
The authors declare no conflict of interest.