Potent Antibacterial Prenylated Acetophenones from the Australian Endemic Plant Acronychia crassipetala

Acronychia crassipetala is an endemic plant species in Australia. Its phytochemistry and therapeutic properties are underexplored. The hexane extract of the fruit A. crassipetala T. G. Hartley was found to inhibit the growth of the Gram-positive bacteria Staphylococcus aureus. Following bio-activity guided fractionation, two prenylated acetophenones, crassipetalonol A (1) and crassipetalone A (2), were isolated. Their structures were determined mainly by NMR and MS spectroscopic analyses. This is the first record of the isolation and structural characterisation of secondary metabolites from the species A. crassipetala. Their antibacterial and cytotoxic assessments indicated that the known compound (2) had more potent antibacterial activity than the antibiotic chloramphenicol, while the new compound (1) showed moderate cytotoxicity.


Introduction
As a result of geographic isolation and a vast array of geographical and environmental habitats, Australia is one of the most megadiverse countries in the world with 84% of terrestrial plants being classified as endemic species [1]. Analysis of Australian flora from Australian tropical habitats showed these regions are particularly rich in plant diversity and species endemism [2]. Despite accounting for only 0.3% of the Australian continent, Queensland's tropical rainforests are internationally recognised as one of the global biodiversity hotspots [3]. This unique ecological resource is a distinct and relatively untapped source of novel and new natural products with therapeutic potential [4]. For example, the Queensland tropical native species Duboisia myoporoides R. Brown is a source of tropane alkaloids including scopolamine, which is an important precursor for the synthesis of scopolamine butylbromide, an anticholinergic and antispasmodic drug with the brand name Buscopan [5]. Another example is a novel indolizidine alkaloid, grandisine A, from the Queensland rainforest tree Elaeocarpus grandis F. Muell, which exhibited binding affinity for the human δ-opioid receptor and has been considered as a potential lead for developing an analgesic agent [6]. More recently, an epoxy-tigliane diterpene, tigilanol tiglate (formerly EBC46), from the Queensland tropical endemic species Fontainea picrosperma C. T. White, has been discovered and shown to have significant anticancer activity [7]. Tigilanol tiglate Medicines Authority [8] as a novel canine therapy for mast cell tumours [9] and is currently in clinical trials to assess its potential as a human anticancer therapeutic [10].
With the success of discovering and developing the novel anticancer natural compound tigilanol tiglate from QBiotics's plant extract library (EcoLogic TM ), a new drug discovery campaign has been launched to discover potent antibacterial natural products [11]. A hexane extract from the fruit of Acronychia crassipetala T. G. Hartley was shown to be active against the Gram-positive (G+ve) bacteria Staphylococcus aureus.
The genus Acronychia belonging to the family Rutaceae comprises over 40 species, which have a broad distribution from India, China, Malesia, New Caledonia to Australia [12]. Some Acronychia spp. have been used in folk medicines of indigenous Asian and Australian populations for the treatments of diarrhoea, asthma, ulcers, rheumatism, fever and parasitic infections [13][14][15]. About 60 compounds including acetophenones, flavonoids, quinoline and acridone alkaloids have been identified from approximately half of the Acronychia spp. plants [12,16]. Several compounds have been found with potential biological activities such as acronine, an anticancer agent from A. baueri [17]; acrophyllodine, an antiarrhythmic agent from A. haplophylla [18]; and acrovestone, an antityrosinase compound from A. pedunculata [19]. The species A. crassipetala is one of the 19 Acronychia spp. recognised in Australia [20]. This shrub is endemic to the wet tropical rainforests of northeastern Queensland and found exclusively at altitudes between 400 and 1250 m [20]. So far, chemical investigation of A. crassipetala has been limited to the study of essential oil extracted from A. crassipetala leaves [20].
This research reports the isolation, structural elucidation, antibacterial and cytotoxic properties of the two prenylated acetophenones from the fruit of A. crassipetala, crassipetalonol A (1) and crassipetalone A (2), of which crassipetalonol A (1) was found as a new compound ( Figure 1).

Results and Discussion
Compound 1 was isolated as a yellowish amorphous powder and had a molecular ion peak at (+) m/z 321.1694 in HR-ESI-MS corresponding to the molecular formula C18H24O5 with seven degrees of unsaturation.
The two isolated acetophenones were tested for their antibacterial activity towards several ESCAPE pathogens (Table 2 and Table S1, Supplementary materials). While crassipetalonol A (1) was found to have low or no activity towards the pathogens at the tested concentration of 156 µM, crassipetalone A (2) potently inhibited the G+ve bacteria S. aureus and Entercoccus faecium with minimum inhibitory concentration (MIC) 75 values of 2.6-20.6 µM. Importantly, compound 2 displayed 2-4 fold more inhibition against S. aureus compared to the antibiotic chloramphenicol. Replacing the acetyl in 2 by the hydroxymethyl ketone in 1 reduced potency against the S. aureus strains 30-fold. Although activity against fungi and G+ve bacteria has previously been reported for acetophenone and its derivatives [24][25][26], the presence of the phenolic hydroxy groups with acidity resulted in increased biological activity by uncoupling oxidative phosphorylation [27]. Moreover, the hydrophilic/lipophilic balance of the molecule was found to play an important role in the penetration of the antibacterial agent through a bacterial cell surface [24]. A certain degree of lipophilicity produced by the iso-prenyl and other substituents in the acetophenone molecule enhanced the antimicrobial activity [26,27]. The higher lipophilicity of compound 2 compared to 1 was predicted by their octanol-water partition coefficient (ClogP) values (4.63 of 2 versus 3.77 of 1) [28]. Therefore, compound 2 could penetrate more easily through the cell wall and exert its bactericidal activity. This study also revealed that the isolated acetophenones selectively inhibited the growth of the tested G+ve bacteria rather than the Gram-negative (G-ve) ones (Table S1, Supplementary materials). These results were in accordance with previous reports of the antibacterial activity of related prenylated acetophenones [26,29,30]. The selective activity of 2 might be related to cell wall disruption or to another specific target present only in G+ve bacteria. Cytotoxicity of compounds 1 and 2 was evaluated using a panel of five human cell lines including immortalised keratinocyte cells (HaCaT), adult dermal fibroblast cells (HDF), neonatal foreskin fibroblast cells (NFF), immortalised embryonic kidney cells (HEK293) and hepatoma cells (HepG2) ( Table 3). The data suggested that crassipetalonol A (1) was 1-5 fold less cytotoxic than crassipetalone A (2). However, comparing the antibacterial and cytotoxic activities suggested that compound 2 had more potential as an antibiotic than compound 1. This difference further supported that the acetyl group contributes significantly to the antibacterial property of the Acronychia-type acetophenone skeleton. Although the selectivity indices between human cancer cells and bacterial cells of compound 2 ranged from 1 to 5, which is relatively low, its potent inhibition against the growth of S. aureus compared to other prenylated acetophenones reported previously [26,[29][30][31] warrants further investigation, including in vivo trials to confirm the value of this compound. In addition, compound 2 could be modified using medicinal chemistry approaches with an aim to further improve the activity/toxicity window.

General Experimental Procedures
IR spectra were obtained on a PerkinElmer Spectrum 400 FT-IR spectrometer (Waltham, MA, USA). NMR spectra were acquired on a Bruker Ascend 400 spectrometer (Billerica, MA, USA) equipped with a 5 mm room temperature probe operating at 400 MHz for 1 H and 100 MHz for 13 C. 1 H and 13 C spectra were referenced to the residual deuterated solvent peaks of DMSO-d 6 at δ H 2.50 and δ C 39.5 ppm. HR-ESI-MS data were acquired on a Sciex X500R Q-TOF mass spectrometer (Framingham, MA, USA). HPLC purifications were performed on a preparative Agilent 1200 system equipped with a diode array detector and processed by ChemStation software (C.01.07). All solvents used for extraction and chromatography were HPLC grade and the H 2 O used was Mili-Q water.

Plant Material
Fruits of Acronychia crassipetala T. G. Hartley (Rutaceae) were sampled from four mature trees growing in lower montane tropical rainforest at Upper Barron, Queensland, Australia, and combined into a single collection for subsequent analysis. Voucher specimens were also collected from each individual tree and held in the QBiotics Limited herbarium (specimen numbers YA1028a to d).

Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentrations (MBC)
MICs and MBCs of plant extracts and fractions were evaluated using standard methodologies carried out in microdilution formats [32,33]. Bacterial isolates were grown aerobically in Mueller Hinton broth (Oxoid) overnight at 37 • C without shaking. After 16 h, the bacteria were centrifuged, the supernatant removed, and the pellet washed in 5 mL of phosphate buffered saline (PBS). The bacteria were then resuspended in Mueller Hinton broth to a concentration of approximately 1 × 10 6 CFU/mL. One hundred microliters of serially diluted plant extract or fractions (max concentration 500 µg/mL) were added to the wells of a 96-well plate (Nunc™ MicroWell™, Sigma Aldrich, Sydney, New South Wales, Australia) containing 100 µL of bacterial suspension. Negative controls (containing 1% DMSO only) were also loaded. In addition, serially diluted plant extracts/fractions (no inoculum) were set up to determine background. The plates were then incubated for 24 h at 37 • C. Following incubation, relative bacterial growth in experimental wells compared to control wells was determined by the optical density of the solution at a wavelength of 600 nm measured by a PerkinElmer EnSpire Multimode plate reader (Waltham, MA, USA). We used MIC 75 (75% inhibition), in conjunction with analysis of dose dependency as the major criteria for identifying fractions/extracts of interest. All extracts were tested in triplicate. For MBC assays, extract/bacterial combinations that displayed significant MIC 75 activity were also plated onto growth media to assess viability. Chloramphenicol was used as a positive control for E. faecium 35667, E. faecium C15, S. aureus 25923, S. aureus 29247, K. pneumoniae 13883, K. pneumoniae 12657, A. baumannii 19606, A. baumannii 17978, E. aerogenes 13048 and E. cloacae 13047. Kanamycin was used as a positive control for P. aeruginosa 10145 and P. aeruginosa 49189. Each compound was assayed in triplicate with at least three biological replicates.

Cell Culture and Reagents
HaCaT (immortalised human keratinocytes), neonatal foreskin fibroblasts (NFF) and HEK293 (immortalised human embryonic kidney cells) were cultured in RPMI media supplemented with 10% foetal calf serum (FCS). HepG2 (human hepatocellular carcinoma) were cultured in DMEM media supplemented with 10% FCS. Adult human dermal fibroblasts (HDF-ThermoFisher Scientific, Waltham, MA, USA) were cultured in Medium 106 (ThermoFisher Scientific, Waltham, MA, USA) supplemented with low serum growth supplement (LSGS) and gentamycin. Cells were maintained in a humidified incubator at 37 • C with 5% CO 2 and passaged using trypsin/versene. All cell lines were confirmed mycoplasma negative prior to use, using MycoAlert (Promega, Madison, WI, USA). HDF and NFF were used between p3 and p10 for all assays.

Cell Growth/Survival Assays
All cells were seeded into clear 96-well plates (Corning #3595, Sigma Aldrich, Sydney, New South Wales, Australia) in 100 µL of media at the following cell concentrations: HaCaT and HEK293, 1000 cells per well; NFF and HDF, 2000 cells per well; and HepG2, 3000 cells per well. After 24 h, the media was removed and 90 µL of fresh media was inserted into each well. Compounds were prepared via serial dilution in the media (to 10× final assay concentration). Vehicle (DMSO)-only controls were also prepared. An amount of 10 µL of compound/vehicle dilutions were subsequently added to cells in duplicate and the resultant plate/s were incubated in a humidified incubator at 37 • C and 5% CO 2 for either 4 (HaCaT), 5 (HEK293) or 7 (HDF, NFF and HepG2) days. Cell growth/viability was measured in each well using a CellTiter 96 ® AQ ueous One Solution Cell Proliferation Assay kit according to the manufacturer's instructions (Promega). Absorbance values were recorded at 490 nm with a H4 Hybrid Synergy plate reader (Biotek). A media-only control was also compiled for background subtraction. Modified absorbance values from compound treated wells were normalised to vehicle-treated samples and the %growth/survival in each sample was determined. The %growth/survival was plotted against Log 10 [Compound] µM to generate absolute IC 50 curves for each compound using PRISM 6.0. Doxorubicin (Sigma Aldrich, Sydney, New South Wales, Australia) was used as a positive control. Each compound was tested in duplicate with at least three biological replicates.

Conclusions
In conclusion, two prenylated acetophenones, crassipetalonol A (1) and crassipetalone A (2), were successfully isolated from the fruit of the Australian endemic plant A. crassipetala. The assessments of their biological activities indicated that the new acetophenone (1) showed relatively high levels of cytotoxicity, while the known compound (2) exhibited relatively high levels of antibacterial activity. With these findings, this study broadens our understanding of the secondary metabolites of the underexplored species A. crassipetala and the therapeutic potential of prenylated acetophenones.