Ethyl Acetate Fraction of Punica granatum and Its Galloyl-HHDP-Glucose Compound, Alone or in Combination with Fluconazole, Have Antifungal and Antivirulence Properties against Candida spp.

Candidiasis is the most common fungal infection among immunocompromised patients. Its treatment includes the use of antifungals, which poses limitations such as toxicity and fungal resistance. Plant-derived extracts, such as Punica granatum, have been reported to have antimicrobial activity, but their antifungal effects are still unknown. We aimed to evaluate the antifungal and antiviral potential of the ethyl acetate fraction of P. granatum (PgEA) and its isolated compound galloyl-hexahydroxydiphenoyl-glucose (G-HHDP-G) against Candida spp. In silico analyses predicted the biological activity of G-HHDP-G. The minimum inhibitory concentrations (MIC) of PgEA and G-HHDP-G, and their effects on biofilm formation, preformed biofilms, and phospholipase production were determined. In silico analysis showed that G-HHDP-G has antifungal and hepatoprotective effects. An in vitro assay confirmed the antifungal effects of PgEA and G-HHDP-G, with MIC in the ranges of 31.25–250 μg/mL and 31.25 ≥ 500 μg/mL, respectively. G-HHDP-G and PgEA synergistically worked with fluconazole against planktonic cells. The substances showed antibiofilm action, alone or in combination with fluconazole, and interfered with phospholipase production. The antifungal and antibiofilm actions of PgEA and G-HHDP-G, alone or in combination with fluconazole, in addition to their effects on reducing Candida phospholipase production, identify them as promising candidates for therapeutics.


Introduction
The incidence of fungal infections is increasing considerably in humans, due to the indiscriminate use of antibiotics, immunosuppressants, and increased use of invasive procedures, such as catheters. These infections can be debilitating, persistent, and result in costly treatments. Many of these microorganisms are natural colonizers of the human microbiota. However, they have an arsenal of factors and virulence properties that are associated with disorders in the host, such as immunodeficiency, trauma, and surgical procedures, which enable them to be opportunistic infections [1][2][3].

In Silico Analysis of the Biological Activities of G-HHDP-G and Its Hepatotoxic Action
The biological activity spectra of G-HHDP-G were determined using an online version of Prediction of Activity Spectra for Substances (PASS) software. Table 1 shows the values obtained for the probable activity (Pa) and probable inactivity (Pi). Several activities were predicted for G-HHDP-G, including anti-infective, antioxidant, hepatoprotective, antiinflammatory, immunostimulant, and antifungal. The highest Pa value was obtained for the anti-infective activity (0.962). The biological activities of FCZ were also determined for comparison. Table 2 shows the biological activity spectra of FCZ. The highest FCZ Pa value was for its antifungal activity (0.726).  The in silico predictions of chemical toxicity are shown in Table 3. The G-HHDP-G did not show any possible damage to the analyzed cytochromes, but FCZ showed probable hepatotoxicity. NT (0.704) * T (0.572) ** NT, non-toxic; T, toxic; * 0.7-0.9, no expected toxicity; ** 0.5-0.7, predicted toxicity.

Antifungal Activity
We evaluated the antifungal activity of PgEA and G-HHDP-G against a panel of two clinical and two reference strains of Candida spp. Both PgEA and G-HHDP-G exhibited antifungal activity against all the tested strains. However, the inhibitory concentrations varied among the isolates. The minimum inhibitory concentration (MIC) ranges were from 31.25 to 250 µg/mL for PgEA, from 31.25 to > 500 µg/mL for G-HHDP-G, and from 4 to 16 µg/mL for FCZ (Table 4).

In Vitro Interaction between PgEA/FCZ and G-HHDP-G/FCZ
The interactions between PgEA/FCZ and G-HHDP-G/FCZ were evaluated using a checkerboard assay. The fractional inhibitory concentration index (FICI) showed a synergistic interaction between PgEA/FCZ ( Figure 1A-D) and G-HHDP-G/FCZ ( Figure 1E,F) against different Candida species. According to the assay, the combination of PgEA and FCZ showed synergistic effects against all Candida isolates. On the other hand, the combination of G-HHDP-G/FCZ had a synergistic effect against two Candida strains (Table 5). Furthermore, there was a drastic reduction in the MIC values of compounds when they were used in combination, compared to the MICs obtained for each compound alone. For example, the MIC values for PgEA against C. albicans ATCC 90028 reduced from 125 µg/mL to 3.9 µg/mL when the fraction was used in combination with FCZ. Against C. albicans CAS, the reduction was from 250 µg/mL to 7.8 µg/mL. In case of G-HHDP-G, the values reduced from 125 µg/mL and 31.25 µg/mL to 31.2 µg/mL and 7.8 µg/mL against C. glabrata ATCC 2001 and C. glabrata FJF, respectively. Even the MIC values for FCZ reduced for most strains when it was used in combination the fraction and compound (Table 5).    Figure 2 shows the effects of PgEA, G-HHDP-G, and FCZ on biofilm form preformed biofilms. All tested strains formed biofilms. PgEA and G-HHDP-G the biofilm formation and significantly interfered with the preformed biofilms albicans and C. glabrata (p < 0.05), both at sub-inhibitory and higher concentration   Figure 2 shows the effects of PgEA, G-HHDP-G, and FCZ on biofilm formation and preformed biofilms. All tested strains formed biofilms. PgEA and G-HHDP-G reduced the biofilm formation and significantly interfered with the preformed biofilms of both C. albicans and C. glabrata (p < 0.05), both at sub-inhibitory and higher concentrations (Figures 2 and 3). FCZ was not able to interfere with the biofilm formation process of the strains, except at MIC concentrations. FCZ also did not interfere with the preformed biofilms of any strain. In contrast, all synergistic concentrations used were able to inhibit both stages of biofilm formation.

Antibiofilm Effect
2 and 3). FCZ was not able to interfere with the biofilm formation process of the strains, except at MIC concentrations. FCZ also did not interfere with the preformed biofilms of any strain. In contrast, all synergistic concentrations used were able to inhibit both stages of biofilm formation.    Effect of PgEA, G-HHDP-G, and FCZ on the preformed biofilm of the clinical isolate C. glabrata FJF, using inhibitory, higher than inhibitory, and synergistic concentrations. *p < 0.05, **p < 0.01, and ***p < 0.0001. Data represent the mean ± SD of three independent experiments carried out in triplicate.

Time Kill-Curve Assay
To evaluate the time-kill activity, this assay was performed over a period of 36 h with C. glabrata ATCC 2001, and C. albicans ATCC 90028 in the presence of G-HHDP-G. The G-HHDP-G concentration of 2 × MIC and 3 × MIC can inhibit C. glabrata ATCC 2001 cell viability from 24-30 h when compared to 1 × MIC and the negative control (4A). Interestingly, for C. albicans ATCC 90028, 3 × MIC (2000 μg/mL) was responsible for completely eliminating viable cells within 12 h of exposure (6Log of cells/mL) ( Figure 4B). The killing activity of G-HHDP-G appears to be dependent on the yeast species or strain; in turn, there is a degree of time and concentration dependence for microbial inhibition. (C) Effect of PgEA, G-HHDP-G, and FCZ on the preformed biofilm of the reference strain C. glabrata 2001, using inhibitory, higher than inhibitory, and synergistic concentrations. (D) Effect of PgEA, G-HHDP-G, and FCZ on the preformed biofilm of the clinical isolate C. glabrata FJF, using inhibitory, higher than inhibitory, and synergistic concentrations. * p < 0.05, ** p < 0.01, and *** p < 0.0001. Data represent the mean ± SD of three independent experiments carried out in triplicate.

Time Kill-Curve Assay
To evaluate the time-kill activity, this assay was performed over a period of 36 h with C. glabrata ATCC 2001, and C. albicans ATCC 90028 in the presence of G-HHDP-G. The G-HHDP-G concentration of 2 × MIC and 3 × MIC can inhibit C. glabrata ATCC 2001 cell viability from 24-30 h when compared to 1 × MIC and the negative control (4A). Interestingly, for C. albicans ATCC 90028, 3 × MIC (2000 µg/mL) was responsible for completely eliminating viable cells within 12 h of exposure ( 6 Log of cells/mL) ( Figure 4B). The killing activity of G-HHDP-G appears to be dependent on the yeast species or strain; in turn, there is a degree of time and concentration dependence for microbial inhibition.

Phospholipase Assay
We measured the extracellular phospholipase activity of C. albicans ATCC 90028, C. albicans CAS, C. glabrata ATCC 2001, and C. glabrata FJF. C. glabrata FJF did not produce phospholipases. The higher the phospholipase activity (as measured according to the calculated phospholipase precipitation zone (Pz)), the lower the Pz value. Thus, both PgEA and G-HHDP-G significantly reduced phospholipase production (p < 0.05), by interfering with the enzyme production levels ( Figure 5 and Table 6).

Phospholipase Assay
We measured the extracellular phospholipase activity of C. albicans ATCC 90028, C. albicans CAS, C. glabrata ATCC 2001, and C. glabrata FJF. C. glabrata FJF did not produce phospholipases. The higher the phospholipase activity (as measured according to the calculated phospholipase precipitation zone (Pz)), the lower the Pz value. Thus, both PgEA and G-HHDP-G significantly reduced phospholipase production (p < 0.05), by interfering with the enzyme production levels ( Figure 5 and Table 6).

Phospholipase Assay
We measured the extracellular phospholipase activity of C. albicans ATCC 90028, C. albicans CAS, C. glabrata ATCC 2001, and C. glabrata FJF. C. glabrata FJF did not produce phospholipases. The higher the phospholipase activity (as measured according to the calculated phospholipase precipitation zone (Pz)), the lower the Pz value. Thus, both PgEA and G-HHDP-G significantly reduced phospholipase production (p < 0.05), by interfering with the enzyme production levels ( Figure 5 and Table 6).

Discussion
In the present study, we report the antifungal activities of PgEA and one of its phenolic compounds G-HHDP-G. P. granatum has attracted the interest of researchers due to its main biological activities, including antioxidant, anti-inflammatory, antibacterial, anticancer, and antiviral [17][18][19][20][21]. Phytochemical analysis performed previously by our research group showed that a richness of phytochemical compounds is present in the PgEA fraction, which corroborates its biological properties [18][19][20]. Among these compounds, we highlight G-HHDP-G in the present study.
In silico analysis of G-HHDP-G indicated a potential antifungal effect of the compound, with a Pa value of 0.692. Pa and Pi values range from 0.000 to 1.000. When Pa is greater than Pi, the compound is believed to be experimentally active. Pa values ranged from 0.5 to 0.7, indicating that the compound will likely show considerable pharmacological effects experimentally [22], which corroborates the data obtained herein. We also evaluated the in silico effects and toxicity of G-HHDP-G and compared the results obtained with those for FCZ, which is the drug of choice for the treatment of fungal infections. The results highlighted low hepatotoxicity of G-HHDP-G in comparison to that of FCZ. Furthermore, the analysis showed that the compound has a potential hepatoprotective effect (Pa = 0.883), which was Antibiotics 2022, 11, 265 9 of 17 not observed in case of FCZ. The examinations were based on the structure-activity ratio of approximately 200,000 compounds and 4000 types of pharmacological activities [23].
Some polyphenolic molecules derived from gallic acid have recently been studied to understand their biological properties. Zhang et al. [24] reviewed 1,2,3,4,6-penta-O-galloylβ-D-glucose, a gallotanin derivative, and drew attention to its attractive pharmacological and physiological activities, such as anticancer, apoptosis-inducing, anti-inflammatory, and antioxidative. Antiviral, antibacterial, and antibiofilm activities have also been attributed to some galotannin derivatives [25,26]. Similarly, Al-Sayed and Esmat [22] verified the hepatoprotective and antioxidant effects of pentagalloyl glucose and other galloyl esters isolated from the extract of Melaleuca styphelioides. However, studies on the antifungal effect of PgEA are scarce, with no reports in the literature about the potential of G-HHDP-G against Candida. Therefore, we decided to demonstrate the promising antifungal activity of PgEA and G-HHDP-G in vitro.
We confirmed the results obtained in silico by estimating the MIC. MIC values for PgEA ranged from 31.25 µg/mL to 250 µg/mL, which was effective against all the tested strains. However, G-HHDP-G was only effective against C. glabrata strains, with MIC values ranging from 31.25 µg/mL to 125 µg/mL. These results are extremely relevant because C. glabrata is intrinsically resistant to azoles [26]. The highest G-HHDP-G value tested against C. albicans was 500 µg/mL, and this concentration was not growth inhibitory. One possibility is that G-HHDP-G has an antifungal effect against C. albicans when combined with one of the other compounds present in PgEA, since this fraction inhibited the growth of this species.
Most of the studies related to the antifungal effects of P. granatum refer to extracts from the fruit, bark, or peel. Lavaee et al. [17] verified that the methanolic and ethanolic extracts of the bark and root of P. granatum had anti-Candida activity. P. granatum peel ethanol extract also showed antifungal activity against oral Candida isolates when tested using the agar well diffusion method [27], and there are few reports on the activities of the leaf extract against Candida. In a recent study [28], the authors found that after fractionation of the hydroalcoholic extract of P. granatum leaves, the ethyl acetate fraction was the richest in polyphenols. However, this fraction did not inhibit the growth of C. albicans, which differs from the results obtained in the present study.
To the best of our knowledge, there are no studies in the scientific literature that verify the antifungal activity of G-HHDP-G from PgEA. It is well known that P. granatum extracts have antifungal activity; however, the compounds responsible for this effect have not yet been identified, and whether they have antivirulence activity is still poorly understood. An interesting investigation was conducted by Brighenti et al. [29], who verified the effect of phenolic compounds from P. granatum, such as punicalin, punicalagin, ellagic acid, and gallic acid, on clinical and reference strains of C. albicans and found punicalagin to be the most active. Other studies have also identified punicalagin as the bioactive compound responsible for the antimicrobial activity of pomegranate peel [30,31].
In this study, both PgEA and G-HHDP-G inhibited most Candida strains at very low MIC values. However, combination therapies are increasingly being used in clinical trials, with the aim of decreasing conventional antimycotic side effects or toxicity and selection of resistant isolates [32]. Therefore, we decided to evaluate whether PgEA and G-HHDP-G, in combination with FCZ, would present a synergistic interaction against Candida strains. The FICI values obtained were very low, and the combinations displayed increased antifungal efficacy against C. albicans and C. glabrata, over the compounds alone. The MIC values of all the compounds against the tested strains reduced drastically by 50%-97% and 75%-88% for PgEA and G-HHDP-G, respectively, when used in combination. Synergistic interactions overcome the limitations of traditional antifungals by reducing the associated side effects and increasing their spectrum of action [33].
Endo et al. [34], when evaluating the synergistic effect of P. granatum fruit extract + FCZ against C. albicans isolates, verified that the MIC values for FCZ decreased two-fold when combined with the fruit extract. Similar results were obtained by Silva et al. [35], who evaluated the effects of the combinations of nystatin and punicalagin against C. albicans. Combined concentrations increased the antifungal efficacy as compared to the compounds alone, and the two of them reduced punicalagin's MIC-50 by four-and eight-fold, increasing Candida inhibition and abrogating the cytotoxicity of punicalagin. These findings support our results since the MIC values for PgEA, G-HHDP-G, and FCZ decreased when they were combined with each other.
An alternative therapy for fungal infection treatment could be the use of compounds with action against. Candida spp. possesses several virulence factors that contribute to its high pathogenicity, including the ability to form biofilms. Candida species are capable of forming biofilms on both biotic (such as plant/animal cells and tissues) and abiotic surfaces (catheters, prosthetic devices, and dentures) [36]. Biofilms protect against cellular phagocytosis and make Candida cells resistant to antifungal drugs [37][38][39]. In our study, we evaluated the effects of PgEA, G-HHDP-G, and FCZ on biofilm formation and preformed biofilms.
PgEA and G-HHDP-G inhibited biofilm formation and reduced preformed biofilms of both C. albicans and C. glabrata, showing greater effectiveness than FCZ. In addition, the synergistic combinations of PgEA/FCZ and G-HHDP-G/FCZ were more efficient against C. albicans and C. glabrata biofilms than the substances alone. These results are extremely relevant because studies involving the effect of P. granatum on Candida biofilms are rare, and it is difficult to find effective compounds that efficiently inhibit biofilms. In a similar study, Bakkiyaraj et al. [40] also observed an antibiofilm action of the methanolic extract of P. granatum and its major compound ellagic acid, but at higher concentrations than those identified in our study. Almeida et al. [41] reported the antibiofilm activity of enriched fractions of Equisetum giganteum and P. granatum associated with and incorporated in a denture adhesive against C. albicans. The mixture was effective against the formation of biofilms on the surface of previously treated polymerized acrylic resin specimens. Villis et al. [42] used the same PgEA fraction as that in this study and verified that this fraction reduced the pre-formed biofilm of some Cryptococcus isolates, while showing better activity than FCZ. We highlight the importance of our findings in significantly reducing Candida biofilms, because these structures are generally associated with the majority of Candida infections and treatment failures, due to their drug-resistant biostructure [43][44][45]. Furthermore, this is the first study to assess the ability of PgEA, G-HHDP-G, and their combinations with FCZ to inhibit Candida biofilms.
Phospholipases are also relevant virulence factors produced by Candida spp. These are enzymes capable of breaking the phospholipid membranes or destroying proteins of the host immune system, and therefore, serve as relevant targets for antivirulence therapies [46]. In general, PgEA and G-HHDP-G significantly reduced phospholipase production (p < 0.5), as compared to FCZ, by interfering with the enzyme production levels. Liu et al. [32] showed that use of licofelone in combination with FCZ decreased the phospholipase activity at low concentrations, as compared to FCZ alone, with the inhibitory effect being positively correlated with the drug concentration. In turn, Nciki et al. [47] tested the effect of tannin-rich extracts in reducing phospholipase production in Candida, which required concentrations that were up to three times higher than those used in this study, suggesting that PgEA and G-HHDP-G have high antivirulence activity.
A limitation of our study is that the action of G-HHDP-G, alone and in association with fluconazole, was evaluated only against species of C. albicans and C. glabrata, since this was our main objective. Thus, there is a need to investigate a greater number of isolates of clinical origin from different anatomical sites and from different species in order to have a broader assessment of the findings of the present study. Additionally, we intend to carry out an evaluation of the effectiveness of the association of the compounds in experimental animal models.

Preparation of PgEA and Isolation/Identification of G-HHDP-G
The hydroalcoholic extract and ethyl acetate fraction of P. granatum leaves were obtained as described by Marques et al. [48] and Pinheiro et al. [18]. The ethyl acetate fraction was subjected to a silica gel chromatography column (230-400 mesh; 8 × 100 cm) and eluted with increasing polarities of mixtures of n-hexane/ethyl acetate and ethyl acetate/methanol, to obtain subfractions. The chromatographic separation resulted in 660 fractions. These fractions were grouped into 6 groups according to the similarity of the chromatographic profile. Group 6 was subjected to another round of chromatography and the compound was isolated in galloyl-HHDP-glucose of > 95% purity.
The structure was determined using HPLC-DAD-ESI-IT/MS analysis, as previously described by Pinheiro et al. [18] (Figure 6). The compound G-HDP-G was characterized with data obtained by fragmentation by mass spectrometry and compared with an authentic standard.

Prediction of the Biological Activities of G-HHDP-G In Silico
The biological activities of G-HHDP-G and FCZ (standard drug) were evaluated using PASS Online [version 2.0, Way2Drug.com© 2011-2022, Moscow, Russia] (www.way2drug.com/passonline/, accessed on September 16th, 2021), which provides several characteristics of the biological action of a substance. The PASS program describes biological activity as "active" (Pa) or "inactive" (Pi), in which the estimated probability varies from zero to one. The chances of finding a particular activity increase when the Pa values are higher and Pi values are lower. The results of PASS prediction were interpreted as follows: (i) only biological activities with Pa > Pi were considered possible for a particular compound; (ii) if Pa > 0.7, the substance is likely to exhibit biological activity and the probability of the compound being an analog of a known pharmaceutical drug is also high; (iii) if 0.5< Pa <0.7, the compound is likely to present biological activity, but the substance is not similar to known drugs; (iv) if Pa < 0.5, the chance of finding a biological activity is lower, but the chance to find a structurally new compound is greater.

Prediction of the Biological Activities of G-HHDP-G In Silico
The biological activities of G-HHDP-G and FCZ (standard drug) were evaluated using PASS Online [version 2.0, Way2Drug.com©2011-2022, Moscow, Russia] (www.way2drug. com/passonline/, accessed on 16 September 2021), which provides several characteristics of the biological action of a substance. The PASS program describes biological activity as "active" (Pa) or "inactive" (Pi), in which the estimated probability varies from zero to one. The chances of finding a particular activity increase when the Pa values are higher and Pi values are lower. The results of PASS prediction were interpreted as follows: (i) only biological activities with Pa > Pi were considered possible for a particular compound; (ii) if Pa > 0.7, the substance is likely to exhibit biological activity and the probability of the compound being an analog of a known pharmaceutical drug is also high; (iii) if 0.5 < Pa < 0.7, the compound is likely to present biological activity, but the substance is not similar to known drugs; (iv) if Pa < 0.5, the chance of finding a biological activity is lower, but the chance to find a structurally new compound is greater.

In Silico Analysis of G-HHDP-G Hepatic Toxicity
To assess the hepatic toxicity of G-HHDP-G and FCZ, we used the Super-CYPsPred [©Structural Bioinformatics Group 2019, Berlin, Germany] (http://insilico-cyp.charite.de/ SuperCYPsPred/, accessed on 16 September 2021) web server, which includes machine learning models based on the random forest algorithm and different types of data sampling methods. The models presented in SuperCYSPred discriminate between inhibitors and non-inhibitors for the five main CYP450 isoforms. Fragment-based and structural similarity approaches were used to evaluate the applicability domain of the models, in addition to predicting a specific compound as active (inhibitor) or inactive (non-inhibitor) for a defined CYP isoform. Following that, 100 µL of Candida inoculum (1 × 10 3 CFU/mL) was added to each well and incubated at 37 • C for 48 h in RPMI-1640 medium. After the incubation period, MIC was defined as the lowest concentration that visibly inhibited fungal growth. FCZ was used as a positive control and RPMI (100 µL) plus standardized inoculum was used as a negative control. The results were obtained from three independent assays performed in triplicate.
One hundred microliters of the inoculum (1 × 10 3 CFU/mL), 50 µL of PgEA or G-HHDP-G, and 50 µL of FCZ were added to 96-well plates. For sterility control, RPMI was used alone (100 µL), and growth was observed in RPMI (100 µL) plus standardized inoculum. Antimicrobial activity was assessed as described for MIC. After data normalization, the FICI was calculated for each compound, according to the general formula: In addition, interaction curves were also constructed. The interaction between compounds was classified as synergism if FICI ≤ 0.5, indifferent if 0.5 > FICI ≤ 4.0, and antagonism if FICI > 4.0 [50]. Three independent assays were performed in triplicate.

Effect of PgEA and G-HHDP-G on Candida Biofilms
Candida biofilms were developed using a slightly modified method [51,52]. To verify the interference of substances on biofilm formation, 200 µL of each substance at MIC, subinhibitory concentrations of MIC/4 and MIC/2, and established synergistic concentrations was used. The interference of substances on preformed biofilms was analyzed using the concentrations of MIC, 4× MIC, 8× MIC, and synergistic concentrations. PgEA and FCZ were tested against C. albicans (ATCC 90028 and CAS) and C. glabrata (ATCC 2001 and FJF). G-HHDP-G was tested against C. glabrata (ATCC 2001 and FJF).
Candida cells previously grown in SDA were transferred to Yeast Nitrogen Base Broth (YNB) (Sigma-Aldrich ® , St. Louis, MO, USA) and incubated for 18 h at 37 • C. The cell pellet was washed three times with sterile phosphate-buffered saline (PBS). A standard cell suspension (1 × 10 6 CFU/mL, 200 µL) was added to 96-well plates and allowed to adhere for 90 min. After the adhesion phase, the microplates were gently washed three times with PBS to remove planktonic cells. To evaluate the interference on biofilm development, 200 µL of PgEA, G-HHDP-G, or FCZ, diluted in YNB + 100 mM glucose, were added to the corresponding wells, and the microplates were incubated for 24 h. For analysis of the interference on preformed biofilm, after the adhesion period, the wells were washed and each well was replaced with 200 µL of YNB. The microplates were then incubated for 24 h. Later, the wells were washed three times, 200 µL of PgEA, G-HHDP-G, and FCZ was added to the wells, and biofilms were incubated for a further 24 h. In all the experiments, biofilms without substances were used as controls.
After the final incubation, biofilms were evaluated for cell viability using 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT; Sigma-Aldrich) method [53]. Briefly, biofilms were washed with PBS, and 100 mL of MTT (5 mg/mL) was added to each sample and incubated for 4 h under light. Supernatants were then removed, 100 mL of Dimethyl Sulfoxide(DMSO) was added to each well, and the samples were incubated for another ten minutes. Readings were performed using a microplate reader (Softmax ® Pro-Molecular Devices General Counsel, USA) at the wavelength of 490 nm. Each experiment was conducted three times in triplicate.

Time Kill-Curve Assay
The time-curve experiments were carried out in plastic tubes with screw caps in RPMI medium (Sigma-Aldrich), with a final volume of 500 µL at 37 • C for 36 h. The cells to the start of the experiment to obtain fungal cultures in early logarithmic phase growth. Cells were suspended in sterile distilled water to achieve a starting inoculum size of 1-5 × 10 6 colony forming units (CFU)/mL and added to the tubes containing G-HHDP-G at concentrations 0.5, 1, 2, and 3 times the MIC. Growth control was also measured by adding the inoculum to tubes containing RPMI medium without drug. Sample for viable counts was taken at 0, 6, 12, 24, 30, and 36 h, plated in triplicate onto Sabouraud dextrose agar (SDA, Difco), and incubated for 24-48 h at 37 • C. After, incubation samples were first diluted in sterile saline (NaCl, 0.9%) and plated in the culture medium. Experiments were performed in duplicate for each isolate at different times. The results of the counts of the yeasts C. albicans ATCC 90028 C. glabrata ATCC 2001 were expressed in Log 10 CFU/mL.

PgEA, G-HHDP-G, and FCZ Interference in Phospholipase Production
The phospholipase activity of Candida spp. was determined using egg yolk agar medium. Both C. albicans and C. glabrata cultures (1 × 10 3 CFU/mL) were treated with PgEA, G-HHDP-G, and FCZ at MIC, MIC/2, and MIC/4. A control group without substances was also included. The cultures were transferred into separate microtubes and incubated at 37 • C for 4 h. Subsequently, 10 µL of the suspension from each tube was inoculated into egg yolk agar medium and the plates were incubated at 37 • C for 72 h. After that, the diameters of the precipitation zones (a) and diameter of the precipitation zone plus diameter of the colony (b) were measured. The Pz was designated as Pz = a/b, as described by Price et al. [54] and Liu et al. [32]. According to this definition, the phospholipase production index was scored and categorized as follows: negative (Pz = 1), very low (Pz = 0.90 to 0.99), low (Pz = 0.80 to 0.89), high (Pz = 0.70 to 0.79), and very high (Pz ≤ 0.69) [50]. Each experiment was conducted three times in triplicate.

Statistical Analysis
All experiments were performed in triplicate, and the values have been expressed as mean ± standard deviation. The results were analyzed using one-way ANOVA, followed by Tukey's test. Statistical analyses were performed using Prism 7.00 software (GraphPad, San Diego, CA, USA), and differences were considered significant when p < 0.05.

Conclusions
The present study provides a substantial advance over recent studies on P. granatum and its compounds, and, to the best of our knowledge, is the first to discover the antifungal effects of G-HHDP-G. We are also pioneers in verifying the synergistic effect of PgEA and G-HHDP-G, in combination with FCZ, against Candida spp. planktonic cells and biofilms. These results indicate that both the PgEA fraction and the compound G-HHDP-G are potential candidates that could serve as antifungal agents and promising synergists with FCZ for the development of new drugs against Candida. However, more in-depth studies need to be conducted to uncover the mechanisms of action of these compounds.