New Antibacterial Secondary Metabolites from a Marine-Derived Talaromyces sp. Strain BTBU20213036

New polyketide-derived oligophenalenone dimers, bacillisporins K and L (1 and 2) and xanthoradone dimer rugulosin D (3), together with four known compounds, bacillisporin B (4), macrosporusone D (5), rugulosin A and penicillide (6 and 7), were isolated from the marine-derived fungus Talaromyces sp. BTBU20213036. Their structures were determined by detailed analysis of HRESIMS, 1D and 2D NMR data, and the absolute configurations were determined on the basis of calculated and experimental electronic circular dichroism (ECD). The antibacterial and antifungal activities of these compounds were tested against Gram-positive—Staphylococcus aureus, Gram-negative—Escherichia coli, and fungal strain—Candida albicans. These compounds showed potential inhibitory effects against S. aureus with minimum inhibitory concentrations ranging from 0.195 to 100 µg/mL.


Introduction
The discovery and introduction into clinics of antibiotics have made great contributions to human health. The most widely applied antibiotics in clinics were discovered in the period between the 1950s and 1960s, while the overuse of antibiotics lead to the emergence of drug-resistant bacteria, which is recognized globally by scientists nowadays [1,2]. The spread of multi-drug resistant microorganisms is getting more and more serious to humans [3][4][5]. It is estimated that 700,000 people died as a result of infections caused by antimicrobial resistant bacteria annually [6]. There is an urgent demand to screen new antibiotics in order to combat the infective diseases caused by drug-resistant bacteria.
Fungi from marine environments have proven to be important pools for structurally unique and biologically diverse natural products [7]. Talaromyces fungi belong to ascomycetous. A number of Talaromyces species have been isolated from marine environments, such as, Talaromyces albobiverticillius, Talaromyces assiutensis, Talaromyces purpureogenus [8][9][10][11][12]. Fungi of Talaromyces genus isolated from marine environments produced a series of bioactive natural products, such as oligophenalenones [11][12][13][14][15], terpenoids [16,17], naphthoquinones [18], spolyene and isocoumarin [19], diphenyl ether derivatives, sesquiterpeneconjugated amino acids [20,21], lactones [22], and ergosterol analog and bisanthraquinone [23]. In the course of our continuing investigation of bioactive natural products from marinederived fungi [24][25][26], the fungal strain Talaromyces sp. BTBU20213036, which was obtained from a mud sample collected from the coastal region of Qingdao, Shandong Province, exhibited antimicrobial activity against Staphylococcus aureus. Fermentation scale-up of this strain was conducted in rice solid media. The study of the chemical constituents of the fermentation materials resulted in the isolation and characterization of three new secondary metabolites, including bacillisporins K and L (1 and 2) and rugulosin D (3), together with solid media. The study of the chemical constituents of the fermentation materials resulte in the isolation and characterization of three new secondary metabolites, includin bacillisporins K and L (1 and 2) and rugulosin D (3), together with four know compounds, bacillisporin B [15], macrosporusone D [27], rugulosin A [28] and penicillid [29] (Figure 1). 1, 2 and 4−6 showed potential antibacterial activities against S. aureu Herein we report the details of isolation, structure elucidation, and antimicrobial activiti evaluation of these compounds.
Compounds 1 and 2 showed almost the same experimental ECD spectra, which were consistent with the reported bacillisporin I [15] and calculated data ( Figure 3). Thus, the configurations of 1 and 2 were determined as 8 R, 9 S, 9 aS, while the configurations of C-1, C-1" and C-2" were not determined. Compounds 1 and 2 were named bacillisporins K and L, respectively. 8', 9' and C-9a' (Table S2). Compounds 1 and 2 showed almost the same experimental ECD spectra, which were consistent with the reported bacillisporin I [15] and calculated data ( Figure 3). Thus, the configurations of 1 and 2 were determined as 8′R,9'S,9'aS, while the configurations of C-1, C-1'' and C-2'' were not determined. Compounds 1 and 2 were named bacillisporins K and L, respectively. Compound 3 was isolated as a brown amorphous powder. The molecular formula of 3 was determined to be C30H22O11 based on the HRESIMS spectrum (m/z [M + H] + 559.1234, calcd for C30H23O11, 559.1235), accounting for twenty degrees of unsaturation ( Figure S17). Figure S18 showed the HPLC profile and UV spectrum of 3. The 1 H, 13  . By comparing the NMR data with those of rugulosin A [28], the structure was deduced as an analogue of rugulosin A (6). Detailed analysis of the NMR data revealed that the sp 2 quaternary carbons of C-1' [δC 186.7] and C-12' Compound 3 was isolated as a brown amorphous powder. The molecular formula of 3 was determined to be C 30 Figure S17). Figure S18 showed the HPLC profile and UV spectrum of 3. The 1 H, 13  ]. By comparing the NMR data with those of rugulosin A [28], the structure was deduced as an analogue of rugulosin A (6). Detailed analysis of the NMR data revealed that the sp 2 quaternary carbons of C-1 [δ C 186.7] and C-12 [δ C 106.8] in rugulosin A were replaced by one ketone carbonyl [δ C 198.8] and one oxygenated sp 3 quaternary carbon [δ C 74.6]. The planar structure of 3 further confirmed by HMBC correlations (Figure 2 and Figure S23, Table S3) from H-2 to C-1 and C-12 . The relative configurations of 3 were deduced by comparing the literature data for 1 H NMR between rugulosin A and 3, the chemical shifts of H-3/H-3 of 3 were δ H 4.27/4.56 with a coupling constant of 5.0 and 4.5 Hz, which were almost the same as those reported for rugulosin A [28]. In the ROESY spectrum (Figure 4 and Figure S24

), correlations between H-3 and H-2 , H-4/H-4 , and between H-3 and H-2 and H-4 were observed, which confirmed
Antibiotics 2022, 11, 222 5 of 9 the relative stereochemistry of 3. By comparison of experimental and calculated ECD spectra (Figure 4), the absolute configurations of 3 were determined as shown in Figure 1 and named rugulosin D.  (Figures 2 and S23, Table S3) from H-2' to C-1' and C-12'. The relative configurations of 3 were deduced by comparing the literature data for 1 H NMR between rugulosin A and 3, the chemical shifts of H-3/H-3′ of 3 were δH 4.27/4.56 with a coupling constant of 5.0 and 4.5 Hz, which were almost the same as those reported for rugulosin A [28].
In the ROESY spectrum (Figures 4 and S24), correlations between H-3′ and H-2′, H-4/H-4′, and between H-3 and H-2 and H-4′ were observed, which confirmed the relative stereochemistry of 3. By comparison of experimental and calculated ECD spectra (Figure 4), the absolute configurations of 3 were determined as shown in Figure 1 and named rugulosin D.   Four known compounds, bacillisporin B [15], macrosporusone D [27], rugulosin A [28] and penicillide [29] were also isolated and characterized by comparing their molecular weight and NMR data with those reported in the literature.
These compounds were tested for antibacterial activities against a panel of pathogens of S. aureus ATCC 25923, Escherichia coli ATCC 25923 and Candida albicans ATCC 10231. Compounds 1, 2 and 4−6 strongly inhibited the growth of S. aureus with MIC values of 12.5, 25, 12.5, 6.25, and 0.195 µg/mL ( Table 3). None of the tested compounds showed inhibitory effects against C. albicans and E. coli at concentration of 100 µg/mL.

General Experimental Procedures
Optical rotations ([α] D ) were measured by using an Anton Paar MCP 200 Modular Circular Polarimeter (Austria) in a 100 × 2 mm cell at 25 • C. CD spectra were recorded on Applied Photophysics Chirascan spectropolarimeter (Surrey, UK). NMR experiments were carried on a Bruker Avance 500 spectrometer at 25 • C with residual solvent peaks as references (DMSO-d 6 : δ H 2.50, δ C 39.52). High resolution ESIMS spectra were measured using an Accurate-Mass-Q-TOF LC/MS 6520 instrument (Santa Clara, CA, USA) in positive ion mode. HPLC was run on an Agilent 1200 Series instrument.

Microbial Material
Strain BTBU20213036 was isolated from a mud sample collected from the intertidal zones of the Yellow Sea in Qingdao, China, and grown on a potato dextrose agar plate at 28 • C for 10 days. Colonies were about 25 mm diam, texture floccose and funiculose, sporulation abundant, dark greyish green, mycelium yellow, no exudate and soluble pigment, colony reverse brown ( Figure S25). The genomic DNA of BTBU20213036 was extracted using DNAquick Plant System (Tiangen, Beijing, China). The ITS sequence of BTBU20213036 was amplified by using a conventional primer pair of ITS4 (5 -TCCTCCGCTTATTGATATGC-3 ) and ITS5 (5 -GGAAGTAAAAGTCGTAACAAGG-3 ). PCR products were sequenced by Beijing Qingke Biotechnology Co., Ltd. (Beijing, China) and the sequence was deposited in GenBank (accession number, OM049426). Strain BTBU20213036 was identified as Talaromyces sp. based on gene sequence analysis of ITS by comparing with sequences from GenBank database using BLAST program ( Figure S26). Alignments and calculations of sequence similarity were carried out using CLUSTAL W [30]. The strain was deposited in Beijing Technology and Business University, Beijing, China.

Fermentation, Extraction and Purification
Talaromyces sp. BTBU20213036 was inoculated on a potato dextrose agar plate and incubated at 28 • C for 7 days. A slice of fungal colony of 1 cm 2 was put into twenty of 1 L conical flasks, each containing 200 g of raw rice, which was soaked in distilled water for 60 min. The inoculated flasks were incubated stationary at 28 • C for 30 days. The fermented materials of Talaromyces sp. BTBU20213036 were extracted three times by EtOAc:MeOH (80: 20), and the organic solvent was evaporated in vacuo at 45 • C to yield brown crude extract (18.4 g). The crude extract was resuspended into 500 mL distilled water and extracted by 500 mL EtOAc (three times). Then EtOAc was evaporated in vacuo at 45 • C to give a dark residue (5.91 g). The EtOAc extract was separated by a reduced pressure silica gel chromatography (50 × 80 mm column, TLC H silica) with a stepwise gradient of 80-100% hexane/CH 2 Cl 2 and then 0-90% MeOH/CH 2 Cl 2 to afford 15 fractions. The eighth fraction was purified on a Sephadex LH-20 column using an elution of CH 2 Cl 2 :MeOH 3 (11.2 mg) and 6 (8.4 mg). The procedure for extraction and compounds isolation was shown in Figure S27

Antibacterial Activity Assays
The antimicrobial activities were performed based on Antimicrobial Susceptibility Testing Standards outlined by the Clinical and Laboratory Standards Institute document M07-A7 (CLSI) [31] and our previous report [26] by using a penal of pathogens of C. albicans ATCC 10231, S. aureus ATCC 25923 and E. coli ATCC 25922. All the tested compounds were dissolved in dimethyl sulfoxide and diluted in two fold. The minimum inhibitory concentrations (MICs) were determined to be the lowest concentration with no visible bacterial in wells.

Conclusions
The chemical investigation of a marine-derived fungus Talaromyces sp. BTBU20213036 resulted in the isolation of three new compounds (1-3), and four previously reported metabolites (4-7). Among them, bacillisporins K and L shared dimeric oligophenaleneone scaffold. Rugulosin D (4) is a dimer of the emodin-type anthraquinone. The absolute configurations of isolated compounds were determined by quantum chemical calculations of ECD. Compounds 1, 2 and 4−7 displayed antibacterial activities against S. aureus with MIC values of 12.5, 25, 12.5, 6.25, 0.195 and 100 µg/mL, respectively. The difference between 3 and 6 is that the hydroxymethine of C-12 in 3 was replaced by a sp 2 quaternary carbon to form an α,β-unsaturated ketene. The α,β-unsaturated ketene moiety enhanced the antibacterial activity of 6 with 64 folds compared to that of 3. The antibacterial activity of 6 (MIC = 0.195 µg/mL) is much stronger than the positive control of vancomycin (MIC = 1 µg/mL), which indicates it could be considered as a lead compound for further investigations into the mechanism and development of antibacterial agents.