Nanomotion Detection-Based Rapid Antibiotic Susceptibility Testing

Rapid antibiotic susceptibility testing (AST) could play a major role in fighting multidrug-resistant bacteria. Recently, it was discovered that all living organisms oscillate in the range of nanometers and that these oscillations, referred to as nanomotion, stop as soon the organism dies. This finding led to the development of rapid AST techniques based on the monitoring of these oscillations upon exposure to antibiotics. In this review, we explain the working principle of this novel technique, compare the method with current ASTs, explore its application and give some advice about its implementation. As an illustrative example, we present the application of the technique to the slowly growing and pathogenic Bordetella pertussis bacteria.


Introduction
According to a WHO report [1], a post-antibiotic era-in which common infections and minor injuries can kill-is far from being an apocalyptic fantasy but a very real possibility for our century. This is due to the fast emergence of multidrug-resistant microbial pathogens, which is caused by the extensive, sometimes unnecessary use of antimicrobials and the lack of interest of pharma in developing new compounds. The cost of antimicrobial resistance (AMR) is projected to increase significantly as some models predict a rise in global casualties from the present figure of one million to 10 million in 2050 [2]. To combat the rise of AMR, a profound understanding of the mechanisms of microbial infections, the development of new diagnostic tools and new antimicrobials are necessary.
To rationalize the use of large spectrum antimicrobial drugs, it is essential to have a rapid and sensitive detection system that identifies the most appropriate drug to fight a given microorganism immediately at the admission of the patient in a medical center. Current antimicrobial susceptibility testing (AST) technologies mostly rely on microbial culturing and thus replication, which can therefore take up to 1 to 3 days [3,4]. As a result of the diagnostic's limited speed, accurate treatment, with effective narrow-range antimicrobial agents, is often replaced by the use of broad-spectrum antimicrobials [5][6][7]. The Table 1. Examples of current phenotypic antibiotic susceptibility testing (AST) methods that are classified according to the measuring principle: culture-based, optical-based, electrical-based and mechanical-based AST methods. MIC: minimal inhibitory concentration.

Method
Characteristics Reference

Optical-based AST methods
Optical tracking of cell division Single-cell division tracking associated with large volume imaging. [39] Multiplexed automated digital microscopy Optical imaging of cells with quantification of growth rates in the presence of antibiotics. [33][34][35] oCelloscope Estimate the growth of bacterial cells with an optical microscope. [36] Single-cell morphological analysis (SCMA) Imaging changes of the morphology of single cells upon antibiotic treatment. [37] Surface plasmon resonance (SPR) A SPR biosensor was used to determine the susceptibility of Staphylococcus aureus clinical isolates. [40] Electrical-based AST methods

Electric resistance
Growth of cells in a microchannel is directly proportional to the measured resistance change. [41] Impedance-based Fast Antimicrobial Susceptibility Test (IFAST) Changes in biophysical properties of bacteria measured by impedance cytometry. [42] Electrochemical Measurement of the change in current due to electrochemical reactions. [43][44][45] Electrical AST (e-AST) Growth of cells is monitored by detecting capacitance change of bacteria bound to 60 aptamer-functionalized capacitance sensors [46] Mechanical-based AST methods

Asynchronous magnetic bead rotation
Detects bacterial growth, based on the rotation of a cluster of magnetic microparticles.

Method Characteristics Reference
16S rRNA identification Influence of antibiotic on growth by measurement of 16S rRNA. [76] ATP bioluminescence ATP quantification as an estimate of the microbial population metabolic activity. [69][70][71][72][73][74] DNA microarrays DNA microarray using 70mer oligonucleotide. probes to detect resistance genes. [49] Real-Time PCR Detection of resistance genes. [50][51][52][53] MALDI-TOF MS and broth dilution Combination of microbial identification with an established AST method. [65,66] MALDI-TOF MS and SILAC Identification of metabolic inactive microorganisms upon antibiotic treatment. [68] Antibiotics 2021, 10, 287 4 of 16 In this review, we will essentially focus on a novel way to characterize the susceptibility of microorganisms to antibiotics. The technique relies on the detection of the nanometric scale oscillations that characterize living cells. Several years ago, our team demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations end as soon the organism dies [77]. Highlighting such minute movements on a single microorganism requires highly sensitive devices such as atomic force microscopes (AFMs). These instruments are particularly adapted to such challenges, since they can detect displacements in the range of 0.1 Å with a temporal resolution in the range of microseconds. As an illustration, the typical distance between two carbon atoms in an organic molecule is about 2 Å. The very first and straightforward application of such a life monitor is rapid AST. The aim of this article is to describe the working principle of these novel devices, to review their contributions to the field of AST and to discuss their future applications.

The Atomic Force Microscope (AFM) and the Cantilever as a Mass Sensor
The atomic force microscope was developed in late 1980s by Binning Quate and Gerber [78]. The instrument aimed to image non-conductive samples with atomic resolution. The microscope consists of a very sharp tip fixed at the end of a soft cantilever that scans the surface of the sample. The tip is maintained in contact with the surface and the deformations of the cantilever are used to reconstruct on a computer screen the 3D topography of the sample. The cantilever deformation is measured by monitoring the reflection angle of a laser beam that bounces off the end of the lever and ends its path on a two-or four-segment photodiode as depicted in Figure 1. ntibiotics 2021, 10, x FOR PEER REVIEW

The Atomic Force Microscope (AFM) and the Cantilever as a Mass Sensor
The atomic force microscope was developed in late 1980s by Binning Quate and ber [78]. The instrument aimed to image non-conductive samples with atomic resolu The microscope consists of a very sharp tip fixed at the end of a soft cantilever that the surface of the sample. The tip is maintained in contact with the surface and the d mations of the cantilever are used to reconstruct on a computer screen the 3D topogr of the sample. The cantilever deformation is measured by monitoring the reflection of a laser beam that bounces off the end of the lever and ends its path on a two-or segment photodiode as depicted in Figure 1. The sample, or in some cases the cantilever, are moved by piezo-electric crystals a sub-Å precision [79]. These crystals convert an electric field that is applied on thei face into a mechanical strain that moves the sample. As an illustration, a potential d ence of 100 V induces a displacement of 25 Å. The device can indifferently operate in uum air or liquid environments. This last ability makes the instrument highly intere for biological applications. In the early days, the instrument was essentially used to i single molecules or individual cells in their "almost native" environment and very numerous microbiological applications of this novel imaging mode appeared in the ature. They essentially focused on the morphological alterations of bacteria followin tibiotic exposure [80][81][82][83][84], or yeast cells upon antifungal treatment [85,86]. In the days, the instrument was essentially used to image single molecules or individual ce their "almost native" environment.
However, very soon it appeared that the device can also measure interaction f between single molecules, monitor the mechanical properties of the sample at a nanom resolution or measure minute changes in the mass of the samples attached to the canti The sample, or in some cases the cantilever, are moved by piezo-electric crystals with a sub-Å precision [79]. These crystals convert an electric field that is applied on their surface into a mechanical strain that moves the sample. As an illustration, a potential difference of 100 V induces a displacement of 25 Å. The device can indifferently operate in vacuum air or liquid environments. This last ability makes the instrument highly interesting for biological applications. In the early days, the instrument was essentially used to image single molecules or individual cells in their "almost native" environment and very soon numerous microbiological applications of this novel imaging mode appeared in the literature. They essentially focused on the morphological alterations of bacteria following antibiotic exposure [80][81][82][83][84], or yeast cells upon antifungal treatment [85,86]. In the early days, the instrument was essentially used to image single molecules or individual cells in their "almost native" environment.
However, very soon it appeared that the device can also measure interaction forces between single molecules, monitor the mechanical properties of the sample at a nanometric resolution or measure minute changes in the mass of the samples attached to the cantilever. A comprehensive review of the different application domains of the AFM can be found in Krieg et al. [87]. The interaction force measurement is achieved by attaching a "ligand" molecule on the substrate and a "receptor" on the tip. To achieve the measurement, the cantilever is brought in close vicinity to the substrate to permit the attachment of the two molecular species. Eventually, the lever is moved afar the surface and sets the newly formed bond under stress which induces a downward bending of the cantilever. When the retraction force of the cantilever overcomes the adhesion between the two molecules, the bond breaks and the cantilever returns to its rest position. The maximal downward bending of the cantilever is directly proportional to the interaction force between the two molecules. The technique can also be used to explore intercellular interaction forces, by attaching mammalian, fungal or bacterial cells onto the lever and/or the substrate [88]. Since bacteria adhesive properties play an important role in biofilm formation, several teams have used AFM to explore this parameter on various surfaces [89][90][91]. The very same technique can be used to monitor the presence of specific molecules on bacterial surfaces [92][93][94].
The mechanical properties measurement of the sample by AFM consists of indenting (i.e., pushing) the tip into the sample and monitoring the deflection of the lever during the process. The harder the sample, the more the lever deforms. The curve that depicts the deformation of the cantilever as a function of the z position of the piezo electric crystal is referred to as a force distance curve (Fdc). By subtracting the Fdc obtained on the sample from another Fdc obtained on a hard substrate obtains a new curve referred to as an indentation curve. This last curve basically indicates the force that is required to indent the tip to a certain depth in the sample. The indentation curve of course depends on numerous other factors such as the shape of the tip [95]. The calculation of the sample's elastic properties, i.e., its Young's modulus, is obtained by fitting the indentation curve with a function such as the one of Hertz [96], Sneddon [97], JKR (Johnson Kendall Roberts) or Tatara [98]. The theoretical foundations of indentation curves are described elsewhere [99,100].
The first measurements of the mechanical properties of microorganisms by AFM were conducted on the archaebacterium Methanospirillum hungatei [101]. This first study was very quickly followed by numerous others involving Magnetospirillum gryphiswaldense [102], Haemophilus influenzae [103], Pseudomonas aeruginosa [104], Klebsiella pneumoniae [105] and Staphylococcus aureus [106]. Thanks to its very high spatial resolution, the AFM can also highlight specific domains that possess different mechanical properties than the rest of the cell wall. Arnal et al. demonstrated this capability of the microscope on Bordetella pertussis [107]. Stiffness inhomogeneities can also be highlighted underneath the bacterial surface by using a peculiar method to process Fdc. This AFM imaging mode is referred to as stiffness tomography [108,109]. It was used to monitor at high resolution stiffness modifications in bacteria upon antibiotic exposure [110,111].
For recent and comprehensive reviews on the use of the AFM to measure mechanical properties of the sample, one can refer to Kasas et al. [112] and Garcia [113], for various contributions of AFM in the field of microbiology, we refer to Garcia [113] and Formosa-Dague et al. [114].
Another type of measurement can also be carried out by the AFM: it involves the monitoring of the cantilever bending upon minute temperature variations [115,116] or the attachment of a molecule onto the lever. The measurement is achieved by monitoring the cantilever resonant frequency or its static bending upon a ligand molecule attachment. The cantilever resonant frequency changes due to the added mass whereas its static bending is induced by a change in the surface stress occurring on one side of the lever [117,118]. These types of devices are extremely sensitive and are widely applied as biosensors [117,[119][120][121][122][123][124][125][126]. They have been used for the detection of very small masses [118,122,[127][128][129], for measuring the buoyant mass of microorganisms and for determining the growth rates of individual microbial cells [130]. They were also applied to detect antibiotic-mucopeptides interaction on cantilever arrays [131]. AFM cantilevers have also been used as nanosensors for living cell studies since they offer many advantages. They are highly sensitive, selective, label-free and provide real-time in situ detection capabilities [132]. Experiment involving single cells have been reported for Escherichia coli [130,133,134], Bacillus subtilis [130,134], Enterococcus faecalis [133], Saccharomyces cerevisiae cells [130,132,133,135], HeLa cells [136], mouse lymphoblasts [130], and human lung carcinoma and mouse lymphocytic leukemia cells [133,137], mouse and human T cells [133]. Cell growth detection has been demonstrated by monitoring resonance frequency changes with immobilized S. cerevisiae and fungal Aspergillus niger spores [138].
The measurement of the cantilever resonant frequency can be quite challenging since the quality factor of the lever dramatically drops in liquids, due to the viscous forces that dampen its oscillations. An elegant workaround of the low-quality factor problem was recently found by Etayash et al. [139]. It consists of designing a channel embedded in the cantilever and of injecting a buffer containing the cells of interest. The cantilever resonant frequency is then measured in air and permits one to monitor the number of organisms in the channel and to distinguish viable and non-viable cells. The drawbacks of the technique are essentially the complexity of fabrication of such levers and the care that has to be taken during the operation to keep the channel clog-and bubble-free.

Nanomotion Detection
In early 2013, we noticed that living organisms deposited onto an AFM cantilever induce nanometric scale oscillations that immediately stop when the organism dies [77]. The setup as well as the measurement procedures are different from the previously mentioned mass and adhesion detection techniques. The organism of interest can be deposited on both sides of the lever as depicted in Figure 2 and the induced oscillations are far below the resonance frequency of the cantilever. The first experiments concerned motile bacteria (Escherichia coli); however, later studies revealed that "non-motile" microorganisms (i.e., those lacking propulsion mechanisms) induce such oscillations of the cantilever, too [140] (Table 3). are essentially the complexity of fabrication of such levers and the care that has to be taken during the operation to keep the channel clog-and bubble-free.

Nanomotion Detection
In early 2013, we noticed that living organisms deposited onto an AFM cantilever induce nanometric scale oscillations that immediately stop when the organism dies [77]. The setup as well as the measurement procedures are different from the previously mentioned mass and adhesion detection techniques. The organism of interest can be deposited on both sides of the lever as depicted in Figure 2 and the induced oscillations are far below the resonance frequency of the cantilever. The first experiments concerned motile bacteria (Escherichia coli); however, later studies revealed that "non-motile" microorganisms (i.e., those lacking propulsion mechanisms) induce such oscillations of the cantilever, too [140] (Table 3).

Microorganisms
Remark Antimicrobial Reference

Escherichia coli
Motile bacterium, rapidly growing bacterium Ampicillin, ceftriaxone, ciprofloxacin [77,[141][142][143] Bacteriophage T7 [143] Bordetella pertussis Non-motile bacterium, slowly growing bacterium Clarithromycin, ampicillin [94] Staphylococcus aureus Non-motile bacterium, rapidly growing bacterium Ciprofloxacin [144] Mycobacterium abscessus Non-motile bacterium, rapidly growing bacterium Rifampicin, isoniazid, amikacin [145] Bacillus Calmette-Guérin Non-motile bacterium, slowly growing bacterium Rifampicin, isoniazid, amikacin [145] Candida albicans Yeast (candidiasis) Caspofungin [144,146] Subsequent extension of the study to larger cells such as yeast, plant and mammalian cells revealed that this phenomenon seems to be general for many organisms living on Earth (Table 4). Very quickly, we have foreseen the utility of such a detection method to conduct rapid antibiotic susceptibility tests and we therefore extended the technique to a large pop-  Staphylococcus aureus Non-motile bacterium, rapidly growing bacterium Ciprofloxacin [144] Mycobacterium abscessus Non-motile bacterium, rapidly growing bacterium Rifampicin, isoniazid, amikacin [145] Bacillus Calmette-Guérin Non-motile bacterium, slowly growing bacterium Rifampicin, isoniazid, amikacin [145] Candida albicans Yeast (candidiasis) Caspofungin [144,146] Antibiotics 2021, 10, 287 7 of 16 Subsequent extension of the study to larger cells such as yeast, plant and mammalian cells revealed that this phenomenon seems to be general for many organisms living on Earth (Table 4). Very quickly, we have foreseen the utility of such a detection method to conduct rapid antibiotic susceptibility tests and we therefore extended the technique to a large population of bacteria such as motile, non-motile, Gram-positive and Gramnegative germs as well to rapidly and slowly growing organisms. In all these experiments, the bacteria were exposed to different antibiotics and the organisms that were sensitive drastically reduced the cantilever oscillation amplitude in the minutes that followed the injection of the drug. Importantly, we also spotted the correlation that exists between the antibiotic concentration and the oscillation amplitude of the exposed microorganisms. This correlation permits one to draw dose-response curves and determine clinically important parameters such as the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) [77]. Table 1 lists the biological samples that were explored with this method. Importantly, other independent groups confirmed these results [142,143,148]. Interestingly, slowly growing bacteria such as B. pertussis or even mycobacteria also responded very quickly to the presence of antibiotics. These results emphasize further the potential of the technique as a rapid antibiotic susceptibility test, especially for tuberculosis and sepsis.

AFM Nanomotion Setup and Measurement
The technique is relatively simple to set up. A detailed procedure describing the preparation measurement and the data processing steps can be found in Venturelli et al. [150]. Briefly, the first step consists of functionalizing a relatively soft (0.06 N/m) AFM cantilever with a cross linking molecule such as glutaraldehyde, paraformaldehyde, APTES ((3-aminopropyl)triethoxysilane) or fibronectin. To ensure a stronger binding, we recommend a suspension of the microorganism in a phosphate-buffered saline (PBS) solution first. Cell membrane parts, various peptides or amino acids present in traditional culture media can hide the attachment spots on the cross-linking molecules. To ensure the attachment, the cantilever is immersed in a droplet containing the bacteria for about 15 min. The sensor is eventually inserted in the analysis chamber of the AFM to start the measurement. Biologically oriented instruments are preferable since they are designed to operate in liquids and permit one to exchange the "imaging" medium during the measurement. Custom built devices such the one depicted in Figure 3 can also be used. tilever is immersed in a droplet containing the bacteria for about 15 min. The sensor is e tually inserted in the analysis chamber of the AFM to start the measurement. Biologic oriented instruments are preferable since they are designed to operate in liquids and pe one to exchange the "imaging" medium during the measurement. Custom built dev such the one depicted in Figure 3 can also be used. The analysis chamber is then filled with the culture medium and the laser beam as as the two-or four-segment photodiodes are adjusted to achieve the highest possible se tivity. The measurement usually starts 5-10 min after the insertion of the cantilever into analysis chamber. This delay permits the liquid medium and the cantilever to reach a t mal steady state. Typical measurements are carried out with a sampling rate of abou The analysis chamber is then filled with the culture medium and the laser beam as well as the two-or four-segment photodiodes are adjusted to achieve the highest possible sensitivity. The measurement usually starts 5-10 min after the insertion of the cantilever into the analysis chamber. This delay permits the liquid medium and the cantilever to reach a thermal steady state. Typical measurements are carried out with a sampling rate of about 20 kHz. Such a high rate is preferred, since it permits one to capture the resonant frequency of the cantilever and to assess the correct position of the laser beam. The oscillations of the lever are recorded for about 15 min in the culture medium before the addition of the antibiotic. Usually, after 10-15 min exposure of bacteria to the drug, the oscillation amplitude drops if the bacteria are sensitive and remains stable or even increases if they are resistant. The experiment can be stopped at this stage; however, we usually inject an additional killing agent such as glutaraldehyde or paraformaldehyde to ensure that the oscillation amplitude drops to zero once all the organisms present on the cantilever are dead.

AFM Nanomotion Data Processing
The data processing step consists of a high pass filtering of the original data set to get rid of the thermal drift of the cantilever. The resulting data are eventually processed to extract the variance in a temporal window of 10 s. The variance of the signal is up to now the most sensitive parameter we found to distinguish between living and dead cells. A trial-and-error process in which we attempted to maximize the difference between signals recorded on living and dead samples determined the size of this 10 s window. It is important to mention that the amplitude of the variance signal directly correlates to the nutrient concentrations in the analysis chamber. This observation significantly extends the technique application domains.
Interestingly, frequency domain analysis did not reveal up to now any preferential peak (i.e., frequency) that we could attribute to the specific bacterial species or a metabolic state. However, we noticed that on the fast Fourier transforms (FFT) of the signal, the largest difference between living and dead cells is located between 0.2 and 100 Hz. This frequency window is very stable among all the living organisms that we, and other groups, explored up to now [142].

Application Example
To illustrate the technique and emphasize its susceptibility and rapidity, we present here an example of nanomotion-based AST applied to a slowly growing non-motile pathogenic bacterium. We selected B. pertussis, which is the causative agent of whooping cough. In this experiment, we monitored this by using one of our custom-made detectors for B. pertussis Tohama I strain nanomotion upon exposure to clarithromycin. The injection of the antibiotic at 5 µg/mL (i.e., MIC) in the analysis chamber induced within minutes a dramatic drop in the variance of the cantilever oscillations (Figure 4).

Advantages and Drawbacks of the AFM Nanomotion AST Technique.
Rapidity is probably the most straightforward advantage of the technique, especially in the case of tuberculosis or pertussis. A reliable AST obtained in about 1-2 h can be a "game changer" in the case of septicemia. The method does not require any previous knowledge about the microorganism to be tested and relies only on the bacterial phenotype. Finally, only a limited number of cells are required to conduct an AST. A few hundred bacteria adhered on the cantilever are enough to obtain a reliable and reproducible signal. This number can drop to one single cell in the case of larger microorganisms such as yeast. In the case of polymicrobial infections, a representative sample composed of the different bacterial species present in the patient has to be adhered to the cantilever. This will allow one to evaluate the action of the antibiotic on the whole population: the lever oscillations will stop only when all organisms present on the cantilever are killed by the antibiotic.
Probably the most limiting drawback of the technique is the need to attach the organisms onto the cantilever. It requires chemicals that cross link the organism to the surface of the lever without compromising the cellular physiology. The technique is based on AFM technology and therefore is relatively expensive. However, custom-made devices as depicted in Figure 3 can advantageously replace commercially available AFMs at a fraction of their prices. Particles or bacteria floating in the analysis chamber can cross the laser beam and modify its intensity on the photodiodes. This can lead to a misinterpretation of the phenomena as a displacement of the cantilever [151]. Vibration isolation is an additional issue since AFMs and nanomotion-dedicated devices are relatively sensitive to environmental vibrations. Up to now, MICs and MBCs were only obtained for bactericidal antibiotics and not for bacteriostatic antibiotics. However, we are convinced that a more detailed analysis of the cantilever oscillations should remove this limitation.

Advantages and Drawbacks of the AFM Nanomotion AST Technique.
Rapidity is probably the most straightforward advantage of the technique, especially in the case of tuberculosis or pertussis. A reliable AST obtained in about 1-2 h can be a "game changer" in the case of septicemia. The method does not require any previous knowledge about the microorganism to be tested and relies only on the bacterial phenotype. Finally, only a limited number of cells are required to conduct an AST. A few hundred bacteria adhered on the cantilever are enough to obtain a reliable and reproducible signal. This number can drop to one single cell in the case of larger microorganisms such as yeast. In the case of polymicrobial infections, a representative sample composed of the different bacterial species present in the patient has to be adhered to the cantilever. This will allow one to evaluate the action of the antibiotic on the whole population: the lever oscillations will stop only when all organisms present on the cantilever are killed by the antibiotic.
Probably the most limiting drawback of the technique is the need to attach the organisms onto the cantilever. It requires chemicals that cross link the organism to the surface of the lever without compromising the cellular physiology. The technique is based on AFM technology and therefore is relatively expensive. However, custom-made devices as depicted in Figure 3 can advantageously replace commercially available AFMs at a fraction of their prices. Particles or bacteria floating in the analysis chamber can cross the laser beam and modify its intensity on the photodiodes. This can lead to a misinterpretation of the phenomena as a displacement of the cantilever [151]. Vibration isolation is an additional issue since AFMs and nanomotion-dedicated devices are relatively sensitive to environmental vibrations. Up to now, MICs and MBCs were only obtained for bactericidal antibiotics and not for bacteriostatic antibiotics. However, we are convinced that a more detailed analysis of the cantilever oscillations should remove this limitation.

Future Developments
We are confident that nanomotion-based AST will play an important role in specific diagnostic domains such as tuberculosis or septicemia. Currently, several AFM-based nanomotion detectors are implemented for evaluation in the Lausanne University Hospital (Prof. G. Greub, Microbiology Department, CHUV, Switzerland). In the case of positive evaluation, they will be included in the routine diagnosis chain. To further increase nanomotion detection-based AST, potential parallel detection systems should be implemented. This would permit one to probe simultaneously, on a single detector, different antibiotics or different bacterial species. This development would also eliminate the need of any previous knowledge of the bacterial species under evaluation. It would permit one to assess the susceptibility of the microorganism for dozens of different antibiotics simultaneously. A universal cross linker would also greatly facilitate the spread of the device in research medical centers. Finally, additional fundamental knowledge about the origin of the nanomotion is mandatory to permit to the technique to further spread in research centers, pharmaceutical companies and hospitals.

Data Availability Statement:
The data presented in this study are available in Figure 4 and on request from the corresponding author.

Acknowledgments:
The authors acknowledge Giovanni Dietler (EPFL, Lausanne, Switzerland) for his highly constructive suggestions in the revision of the manuscript. R.W. and S.K. acknowledge the Research Council of the Vrije Universiteit Brussel (Belgium) to support the International Joint Research Group (IJRG) VUB-EPFL BioNanotechnology and NanoMedicine (NANO).

Conflicts of Interest:
The authors declare no conflict of interest.

Appendix A
B. pertussis Tohama I strain (Collection of Institute Pasteur, Paris, France -CIP 8132-) was grown in Bordetella agar plates with charcoal, supplemented with 7% horse blood, supplied by BD Difco for 48 h at 37 • C. The colonies were cultured for 48 h and then inoculated in 100 mL Erlenmeyers flasks containing 30 mL of Stainer-Sholte (SS) liquid medium. This culture was incubated for 24 h at 37 • C with shaking at 160 rpm. The initial concentration at 650 nm was OD650: 0.2; after 24 h of growth, the culture was centrifuged at 8000 g for 5 min and washed four times with sterile phosphate-buffered saline (PBS). Lastly, bacteria were washed three times in PBS; between each rinse they were sedimented by centrifugation at 8500 rpm for 5 min and were finally suspended in SS liquid medium to get a final high-density bacterial suspension (OD595: 0.5 with 100 µL of suspension diluted in 1 mL of PBS).
Cantilevers of silicon nitride with a force constant of 0.06-0.12 N/m were incubated for 10 min with a drop (10 µL) of 0.5% (v/v) glutaraldehyde, rinsed with ultrapure water, dried, and incubated with 10 µL of the high-density bacterial suspension. Three washes with PBS were performed to eliminate the non-adhering or poorly adhered bacteria. These cantilevers with adhered bacteria were introduced into the analysis chamber of the nanomotion detector to test different liquid conditions. Before starting the measurements, the analysis chamber was allowed to stabilize for a short time. Initially, the vibration of the cantilever with B. pertussis adhered bacteria was analyzed in SS medium and then the liquid was replaced by fresh SS medium with antibiotic and a new data record was carried out, at the room temperature. The antimicrobial used was clarithromycin (Sigma-A3487) in a concentration of 5 µg/mL. The deflection signal was collected at a sampling frequency of 20 kHz (20000 data points by seconds). Cantilever deflection and variance of the deflection signal were calculated to define the amplitude of the sensor's movements. The evident outliers were removed in cases of inevitable non-biological signal. The information was recorded and analyzed using custom software written in LabVIEW (National Instruments) and the Matlab R2013b software.