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Biosensors 2014, 4(4), 329-339;

Biosensor Applications of MAPLE Deposited Lipase

Istituto Motori—CNR, via G. Marconi 8, 80125 Napoli, Italy
Department of Physics, University of Naples "Federico II", Complesso Universitario di Monte Sant'Angelo, via Cintia, 80126 Napoli, Italy
SPIN—CNR, c/o Dipartimento di Fisica, Complesso Universitario di Monte Sant'Angelo, via Cintia, 80126 Napoli, Italy
Department of Chemical Engineering, Materials and Industrial Production, University of Naples "Federico II", Piazzale V. Tecchio 80, 80126 Napoli, Italy
DIMI—Dipartimento di Ingegneria Meccanica ed Industriale, University of Brescia, via Branze 38, 25123 Brescia, Italy
Author to whom correspondence should be addressed.
Received: 6 August 2014 / Revised: 19 September 2014 / Accepted: 1 October 2014 / Published: 3 October 2014
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Matrix Assisted Pulsed Laser Evaporation (MAPLE) is a thin film deposition technique derived from Pulsed Laser Deposition (PLD) for deposition of delicate (polymers, complex biological molecules, etc.) materials in undamaged form. The main difference of MAPLE technique with respect to PLD is the target: it is a frozen solution or suspension of the (guest) molecules to be deposited in a volatile substance (matrix). Since laser beam energy is mainly absorbed by the matrix, damages to the delicate guest molecules are avoided, or at least reduced. Lipase, an enzyme catalyzing reactions borne by triglycerides, has been used in biosensors for detection of β-hydroxyacid esters and triglycerides in blood serum. Enzymes immobilization on a substrate is therefore required. In this paper we show that it is possible, using MAPLE technique, to deposit lipase on a substrate, as shown by AFM observation, preserving its conformational structure, as shown by FTIR analysis. View Full-Text
Keywords: MAPLE; lipase; biosensors MAPLE; lipase; biosensors

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Califano, V.; Bloisi, F.; Aronne, A.; Federici, S.; Nasti, L.; Depero, L.E.; Vicari, L.R.M. Biosensor Applications of MAPLE Deposited Lipase. Biosensors 2014, 4, 329-339.

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