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Tuning the Surface Charge of Self-Assembled Polydiacetylene Vesicles to Control Aggregation and Cell Binding

Department of Bioengineering, University of Texas at Arlington, 500 UTA Blvd., Arlington, TX 76013, USA
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Biosensors 2020, 10(10), 132; https://doi.org/10.3390/bios10100132
Received: 24 August 2020 / Revised: 18 September 2020 / Accepted: 21 September 2020 / Published: 24 September 2020
(This article belongs to the Section Biosensor Materials)
Polydiacetylene vesicles of various compositions were assembled using a two-part mixture of 10,12-pentacosadiynoic acid (PCDA) and ethylenedioxy-bis-ethylamine (EDEA)-labeled PCDA in order to control surface charge and stability within a desired pH range. Investigation of the interaction of the vesicles with mammalian cells as a function of surface charge was carried out and identified a clear correlation in cell–vesicle association and corresponding cell death for vesicles with positive surface charge. The binding behavior of the vesicles was found to be tunable by regulating the proportion of anionic PCDA relative to cationic PCDA–EDEA content within vesicles as to control the surface charge as a function of pH. Association of vesicles with cells thus depended on the corresponding charge of the vesicles and cell surface. The prospect of this work may serve as a step toward future vesicle designs to allow triggered uptake of vesicles locally within low pH tumor microenvironments. View Full-Text
Keywords: polydiacetylene; vesicles; surface charge; pH polydiacetylene; vesicles; surface charge; pH
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MDPI and ACS Style

David Nelson, A.; Shiveshwarkar, P.; Lim, B.; Rojas, G.; Abure, I.; Shrestha, A.; Jaworski, J. Tuning the Surface Charge of Self-Assembled Polydiacetylene Vesicles to Control Aggregation and Cell Binding. Biosensors 2020, 10, 132. https://doi.org/10.3390/bios10100132

AMA Style

David Nelson A, Shiveshwarkar P, Lim B, Rojas G, Abure I, Shrestha A, Jaworski J. Tuning the Surface Charge of Self-Assembled Polydiacetylene Vesicles to Control Aggregation and Cell Binding. Biosensors. 2020; 10(10):132. https://doi.org/10.3390/bios10100132

Chicago/Turabian Style

David Nelson, Anthony, Priyanka Shiveshwarkar, Butaek Lim, Gumaro Rojas, Izele Abure, Anura Shrestha, and Justyn Jaworski. 2020. "Tuning the Surface Charge of Self-Assembled Polydiacetylene Vesicles to Control Aggregation and Cell Binding" Biosensors 10, no. 10: 132. https://doi.org/10.3390/bios10100132

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