A Highly Efficient and Durable Fluorescent Paper Produced from Bacterial Cellulose/Eu Complex and Cellulosic Fibers

The general method of producing fluorescent paper by coating fluorescent substances onto paper base faces the problems of low efficiency and poor durability. Bacterial cellulose (BC) with its nanoporous structure can be used to stabilize fluorescent particles. In this study, we used a novel method to produce fluorescent paper by first making Eu/BC complex and then processing the complex and cellulosic fibers into composite paper sheets. For this composting method, BC can form very stable BC/Eu complex due to its nanoporous structure, while the plant-based cellulosic fibers reduce the cost and provide stiffness to the materials. The fluorescent paper demonstrated a great fluorescent property and efficiency. The ultraviolet absorbance or the fluorescent intensity of the Eu-BC fluorescent paper increased with the increase of Eu-BC content but remained little changed after Eu-BC content was higher than 5%. After folding 200 times, the fluorescence intensity of fluorescent paper decreased by only 0.7%, which suggested that the Eu-BC fluorescent paper has great stability and durability.


Introduction
Europium (Eu) element has one of the best photoluminescent properties among rare earth elements. Due to the 4f→4f transition, Eu 3+ ions can convert ultraviolet light into visible light and have been widely used in fluoroimmunoassay [1,2]. The antenna effect induced by the chelation between Eu 3+ and negatively charged ligand results in an even stronger photoluminescent performance for ligand/Eu 3+ complexes. The complexes of various ligands, e.g., β-diketone, chelated with Eu 3+ can be used as photoreceptors, superconductors, magnetic materials, catalysts, fluorescent probes, and light-emitting diodes [3][4][5][6][7][8].
Complexes of polymer ligands and rare earth elements are considered as high-efficiency light-emitting materials because of their strong photoluminescent ability, easy color mixing, low temperature sensitivity, and high thermal stability [9]. In the complex of cholesterol-g-poly (N-isopropylacrylamide) (PNIPAM) and Eu 3+ , the oxygen-nitrogen coordination between amide group and Eu 3+ provides a strong fluorescent characteristic for Eu 3+ [10]. Those ligands with functional groups containing strong electron-donating groups, e.g., hydroxyls, carboxyls, amides, and nitriles, especially have strong chelation effects with Eu 3+ ions or other rare earth elements. Carboxymethyl cellulose (CMC) or cholesterol-g-poly(N-isopropylacrylamide) were used to complex with Eu or Tb, and they showed strong photofluorescent properties [11][12][13][14]. Hybridized nanofibers were produced between polyacrylonitrile and Eu 3+ , which had one-dimensional nanostructure and high specific surface area, showing excellent fluorescent properties [1]. However, most of these photofluorescent complexes BC/Eu complex first and then processing the complex and cellulosic fibers into paper sheets. This method incorporates the fluorescent complexes as part of the cellulosic fiber matrix and thus may improve the stability and durability of fluorescent paper.

Materials
Gluconacetobacter xylinus ATCC23767 was obtained from Nanjing High Tech University Biological Technology Research Institute Co., Ltd. (Nanjing, China) and used to produce the bacterial cellulose (BC) pellicles according to the static fermentation method [20,21]. The degree of polymerization (DP) and Segal crystallinity index (CI) of BC was predetermined as 1081 and 96.0%, respectively [20,21].
Europium trioxide (Eu 2 O 3 ), hydrochloric acid, and sodium hydroxide were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). All other chemicals are analytical grade chemicals.

Preparation of EuCl 3 ·6H 2 O
Eu 2 O 3 (0.727 g) was completely dissolved in 6.0 mol/L HCl solution. The mixture was placed in boiling water bath for 30 min to evaporate the unreacted HCl. The solution was then diluted with deionized water to volume 100 mL to adjust the concentration to 0.0416 mol/L.

Preparation of Eu-BC
Approximately 20 g of wet BC pellicles (moisture content = 98.5%) was disintegrated by a lab homogenizer (SKG 1246, Foshan, China) for 20 s in 130 mL of water. The water was then filtered to obtain the disintegrated BC. The pH of the EuCl 3 ·6H 2 O solution was adjusted to 5-6 with 1 M NaOH. The disintegrated BC was then added slowly to the EuCl 3 ·6H 2 O solution at 45 • C under a magnetic stirring (400 rpm). The pH of mixture was adjusted to about 7 with 1 M NaOH. Finally, the mixture was refluxed at 70 • C for 1 h 30 min to prepare Eu-BC.

Manufacture of Fluorescent Paper
A vacuum filtering method was used to prepare the fluorescent paper because at high EuBC addition it would take a very long time at the water drainage step for the standard TAPPI method. In detail, bleached sugarcane bagasse pulps (BSBP) and Eu-BC were composited with different ratios, i.e., 1%, 2%, 5%, 10%, and 20% (the proportion of Eu-BC based on total paper dry weight). The mixture was homogenizer for 20 s and the mixture was filtered by a sand core filter device. After water was drained, the paper sheet was formed. Finally, the paper was dried in an oven at 9 • C for 50 min.

Characterization of Eu-BC and Fluorescent Paper
Nitrogen adsorption-desorption isotherm measurements were performed on an ASAP 2460 (Micromeritics, Norcross, GA, USA) volumetric adsorption analyzer at 77 K. The Brunauer-Emmett-Teller (BET) method was utilized to calculate the specific surface area of each sample. All samples were degassed at 120 • C at 0.05 atm for 6 h before the measurement began.
Fluorescent paper sheets or Eu-BC were taken under an AGL-9406 UV lamp for excitation at 256 nm. The ultraviolet light absorption of the samples was measured by solid-state ultraviolet spectrophotometer (UV-2450, SHIMADZU, Kyoto, Japan). The fluorescence spectra of the samples were recorded by a steady-state transient fluorescence spectrometer (FluoroMax-4, HORIBA Jobin Yvon, Longjumeau, France).
The Eu content of the paper was measured by an atomic absorption spectrometer (Z-2000, Hitachi, Tokyo, Japan). The surface morphologies of unmodified BC, Eu-BC, Eu-BC fluorescent papers were observed by a scanning electron microscope (SEM) (Merlin, Zeiss, Munich, Germany); X-ray photoelectron spectroscopy (XPS) was conducted on the fluorescent paper by a X-ray photoelectron spectrometer (Kratos Axis Ulra DLD, Kratos Analytical, Manchester, UK) to analyze the valence and binding energy of the Eu ions.

Characterization of Eu-BC and Fluorescent Paper
Bacterial cellulose (BC) is a natural cellulosic material with nanoporous structure, which provides the advantages to stably support functional nanoparticles. The BC used in this study was prepared by static fermentation method instead of agitated method because BC prepared by static method provides paper with better mechanical properties [21]. The BC had a specific surface area of 41.69 m 2 /g and a pore volume of 0.1598 cm 3 /g ( Table 1). As can be seen from the SEM image (Figure 1a), BC is composed of ultrafine and reticular nanofibrils demonstrating a large specific surface area. Eu-BC complex was prepared by adsorbing Eu 3+ onto the nanoporous structure of BC. The total Eu content of Eu-BC was 32.3 wt% (Table 1), which suggested that the Eu ions were successfully adsorbed onto BC. The XPS spectrum of Eu-BC showed that the binding energies of the two peaks were close to 1126 and 1155 eV, which belonged to the Eu 3+ valence state and suggested that all the Eu in BC was still in Eu 3+ forms ( Figure 2a). The XPS spectrum indicated that Eu 3+ ions were successfully coordinated with BC to form a Eu-BC complex. Specific surface area and pore volume of Eu-BC decreased greatly to 6.84 m 2 /g and 0.0214 cm 3 /g, indicating Eu 3+ formed uniform complex with BC and occupied most of pores on BC surface. As demonstrated by SEM image (Figure 1b), Eu-BC complex was evenly distributed or dispersed among the BC matrix. Eu-BC complex was evenly dispersed because of the nanoporous structure of BC, and it also indicated the formation of nanosized complex particles. The Bleached sugarcane bagasse pulp (BSBP) was composited with Eu-BC to produce fluorescent papers with different Eu-BC contents. The Eu content in Eu-BC fluorescent paper as determined by atomic absorption spectrometry was proportional to the amount of Eu-BC added to the production of the fluorescent paper, demonstrating a uniform adsorption of Eu ions onto BC ( Table 1). Most of the Eu elements in the fluorescent paper were still in the valence state of 3+ (Figure 2b), facilitating the photofluorescence of the fluorescent paper. The fluorescent paper showed red fluorescence under ultraviolet light irradiation at 256 nm ( Figure 3), indicating that the energy of ultraviolet light absorbed is effectively transferred to Eu 3+ through the "antenna effect", The Bleached sugarcane bagasse pulp (BSBP) was composited with Eu-BC to produce fluorescent papers with different Eu-BC contents. The Eu content in Eu-BC fluorescent paper as determined by atomic absorption spectrometry was proportional to the amount of Eu-BC added to the production of the fluorescent paper, demonstrating a uniform adsorption of Eu ions onto BC ( Table 1). Most of the Eu elements in the fluorescent paper were still in the valence state of 3+ (Figure 2b), facilitating the photofluorescence of the fluorescent paper. The fluorescent paper showed red fluorescence under ultraviolet light irradiation at 256 nm (Figure 3), indicating that the energy of ultraviolet light absorbed is effectively transferred to Eu 3+ through the "antenna effect", making the fluorescent paper glow red [11][12][13][14]. It can be seen from the SEM images that almost all the bagasse fibers were uniformly covered by Eu-BC (Figure 1c-e). The reticular structure of BC can also be identified on fiber surface (Figure 1f).

Photofluorescent Properties of Eu-BC and Eu-BC Paper
As can be seen from Figure 4, there were two peaks in the Eu-BC excitation spectrum, 305 nm and 312 nm (Figure 4a). The 305 nm wavelength was selected to excite fluorescent papers of different Eu-BC contents to obtain a fluorescence emission spectrum. With the excitation at 305 nm wavelength, the fluorescent paper produced radiation emission at 605 nm and 618 nm (Figure 4b), which should correspond to Eu 3+ magnetic dipole transition of 5 D 0 → 7 F 1 and electric dipole transition of 5 D 0 → 7 F 2 [1]. The peaks of Figure 4b suggested that the emission intensity of fluorescent paper below 5% Eu-BC content increases with the increases of Eu-BC content. The emission intensity of fluorescent paper conducted no more increases when the EU-BC content in fluorescent paper was greater than 5%. Nanomaterials 2019, 9, x FOR PEER REVIEW 7 of 11 which should correspond to Eu 3+ magnetic dipole transition of 5 D0 → 7 F1 and electric dipole transition of 5 D0→ 7 F2 [1]. The peaks of Figure 4b suggested that the emission intensity of fluorescent paper below 5% Eu-BC content increases with the increases of Eu-BC content. The emission intensity of fluorescent paper conducted no more increases when the EU-BC content in fluorescent paper was greater than 5%. By evaluating the solid-state ultraviolet absorption spectrum (Figure 5a), the fluorescent paper had two absorbance peaks at the wavelength of ~230 and ~280 nm. The absorbance of 1%, 2%, and 5% Eu-BC fluorescent paper successively increased with the increase of Eu-BC content. However, the absorbance of 5%, 10%, and 20% Eu-BC fluorescent paper remained almost unchanged. This result was consistent with the fluorescence excitation spectrum, showing that after Eu-BC content exceeds 5% the fluorescent property would not change for the fluorescent paper. The structure of 5% Eu-BC and 20% Eu-BC fluorescent paper were shown in Figure 6. For the 5% Eu-BC paper, the reticular structure of BC can still be seen, but for the 20% Eu-BC paper, the paper was all covered with Eu-BC complex packed full with Eu elements, in which some Eu-BC complexes were observed to be stacked with each other. Many Eu-BC complexes or Eu particles were squeezed or buried inside the middle of the composite, which made them hard to be stimulated by ultraviolet light. This statement can be proved by the surface area, pore volume, and pore size data of EuBC paper (Table 1). For 1% EuBC paper, the cellulosic fibers were not entirely covered with EuBC, so it had relatively high pore volume and pore size; for 5% EuBC paper, the cellulosic fibers were largely covered with EuBC, so its pore volume and pore size decreased; for 20% EuBC paper, many EuBC stacked together, building some now pores between layers leading to By evaluating the solid-state ultraviolet absorption spectrum (Figure 5a), the fluorescent paper had two absorbance peaks at the wavelength of~230 and~280 nm. The absorbance of 1%, 2%, and 5% Eu-BC fluorescent paper successively increased with the increase of Eu-BC content. However, the absorbance of 5%, 10%, and 20% Eu-BC fluorescent paper remained almost unchanged. This result was consistent with the fluorescence excitation spectrum, showing that after Eu-BC content exceeds 5% the fluorescent property would not change for the fluorescent paper. which should correspond to Eu 3+ magnetic dipole transition of 5 D0 → 7 F1 and electric dipole transition of 5 D0→ 7 F2 [1]. The peaks of Figure 4b suggested that the emission intensity of fluorescent paper below 5% Eu-BC content increases with the increases of Eu-BC content. The emission intensity of fluorescent paper conducted no more increases when the EU-BC content in fluorescent paper was greater than 5%. By evaluating the solid-state ultraviolet absorption spectrum (Figure 5a), the fluorescent paper had two absorbance peaks at the wavelength of ~230 and ~280 nm. The absorbance of 1%, 2%, and 5% Eu-BC fluorescent paper successively increased with the increase of Eu-BC content. However, the absorbance of 5%, 10%, and 20% Eu-BC fluorescent paper remained almost unchanged. This result was consistent with the fluorescence excitation spectrum, showing that after Eu-BC content exceeds 5% the fluorescent property would not change for the fluorescent paper. The structure of 5% Eu-BC and 20% Eu-BC fluorescent paper were shown in Figure 6. For the 5% Eu-BC paper, the reticular structure of BC can still be seen, but for the 20% Eu-BC paper, the paper was all covered with Eu-BC complex packed full with Eu elements, in which some Eu-BC complexes were observed to be stacked with each other. Many Eu-BC complexes or Eu particles were squeezed or buried inside the middle of the composite, which made them hard to be stimulated by ultraviolet light. This statement can be proved by the surface area, pore volume, and pore size data of EuBC paper (Table 1). For 1% EuBC paper, the cellulosic fibers were not entirely covered with EuBC, so it had relatively high pore volume and pore size; for 5% EuBC paper, the cellulosic fibers were largely covered with EuBC, so its pore volume and pore size decreased; for 20% EuBC paper, many EuBC stacked together, building some now pores between layers leading to the increase in pore volume and pore size (Table 1). Similar results were also found in the studies of The structure of 5% Eu-BC and 20% Eu-BC fluorescent paper were shown in Figure 6. For the 5% Eu-BC paper, the reticular structure of BC can still be seen, but for the 20% Eu-BC paper, the paper was all covered with Eu-BC complex packed full with Eu elements, in which some Eu-BC complexes were observed to be stacked with each other. Many Eu-BC complexes or Eu particles were squeezed or buried inside the middle of the composite, which made them hard to be stimulated by ultraviolet light. This statement can be proved by the surface area, pore volume, and pore size data of EuBC paper (Table 1). For 1% EuBC paper, the cellulosic fibers were not entirely covered with EuBC, so it had relatively high pore volume and pore size; for 5% EuBC paper, the cellulosic fibers were largely covered with EuBC, so its pore volume and pore size decreased; for 20% EuBC paper, many EuBC stacked together, building some now pores between layers leading to the increase in pore volume and pore size (Table 1). Similar results were also found in the studies of poly(acrylonitrile)/Eu 3+ complex that high Eu 3+ does not necessary lead to improved emission intensity due to the stacking of emission centers [1]. This explains the fluorescence intensity or ultraviolet absorbance remaining constant when Eu-BC is over 5%. It also indicated that 5% is the most economical amount for Eu-BC to composite with cellulosic fibers. poly(acrylonitrile)/Eu 3+ complex that high Eu 3+ does not necessary lead to improved emission intensity due to the stacking of emission centers [1]. This explains the fluorescence intensity or ultraviolet absorbance remaining constant when Eu-BC is over 5%. It also indicated that 5% is the most economical amount for Eu-BC to composite with cellulosic fibers. Figure 6. SEM images of (a) 5% Eu-BC fluorescent paper and (b) 20% Eu-BC fluorescent paper at 10K× magnification.

Stability and Durability of Fluorescent Paper
To investigate the stability and durability of fluorescent paper, 5% Eu-BC fluorescent paper and 20% Eu-BC fluorescent paper were repeatedly folded 200 times. From the ultraviolet absorbance spectrum in Figure 5b, it can be found that the absorbance peaks decreased only slightly after folding 200 times. The absorbance of 5% Eu-BC fluorescent paper decreased by only 0.05 after 200 folds, while the 20% Eu-BC paper decreased by nearly 0.1. The fluorescence intensity at 618 nm emission changes after the folding of the fluorescent paper were shown in Figure 7. The intensity of the fluorescent paper only decreased by ~ 0.7% after 200 folds. This result suggested that the fluorescent paper has great stability and durability, and 5% Eu-BC fluorescent paper is even better than the 20% Eu-BC fluorescent paper. It suggested that the nanoporous and reticular structure of BC can help to form very stable complex with Eu ions, by preventing the Eu particles from leaching or aggregating.

Stability and Durability of Fluorescent Paper
To investigate the stability and durability of fluorescent paper, 5% Eu-BC fluorescent paper and 20% Eu-BC fluorescent paper were repeatedly folded 200 times. From the ultraviolet absorbance spectrum in Figure 5b, it can be found that the absorbance peaks decreased only slightly after folding 200 times. The absorbance of 5% Eu-BC fluorescent paper decreased by only 0.05 after 200 folds, while the 20% Eu-BC paper decreased by nearly 0.1. The fluorescence intensity at 618 nm emission changes after the folding of the fluorescent paper were shown in Figure 7. The intensity of the fluorescent paper only decreased by~0.7% after 200 folds. This result suggested that the fluorescent paper has great stability and durability, and 5% Eu-BC fluorescent paper is even better than the 20% Eu-BC fluorescent paper. It suggested that the nanoporous and reticular structure of BC can help to form very stable complex with Eu ions, by preventing the Eu particles from leaching or aggregating. poly(acrylonitrile)/Eu 3+ complex that high Eu 3+ does not necessary lead to improved emission intensity due to the stacking of emission centers [1]. This explains the fluorescence intensity or ultraviolet absorbance remaining constant when Eu-BC is over 5%. It also indicated that 5% is the most economical amount for Eu-BC to composite with cellulosic fibers. Figure 6. SEM images of (a) 5% Eu-BC fluorescent paper and (b) 20% Eu-BC fluorescent paper at 10K× magnification.

Stability and Durability of Fluorescent Paper
To investigate the stability and durability of fluorescent paper, 5% Eu-BC fluorescent paper and 20% Eu-BC fluorescent paper were repeatedly folded 200 times. From the ultraviolet absorbance spectrum in Figure 5b, it can be found that the absorbance peaks decreased only slightly after folding 200 times. The absorbance of 5% Eu-BC fluorescent paper decreased by only 0.05 after 200 folds, while the 20% Eu-BC paper decreased by nearly 0.1. The fluorescence intensity at 618 nm emission changes after the folding of the fluorescent paper were shown in Figure 7. The intensity of the fluorescent paper only decreased by ~ 0.7% after 200 folds. This result suggested that the fluorescent paper has great stability and durability, and 5% Eu-BC fluorescent paper is even better than the 20% Eu-BC fluorescent paper. It suggested that the nanoporous and reticular structure of BC can help to form very stable complex with Eu ions, by preventing the Eu particles from leaching or aggregating.

Conclusions
Fluorescent paper was prepared by compositing the Eu-BC complex with sugarcane bagasse pulps (BSBP). The fluorescent paper demonstrated a great fluorescent property and efficiency, i.e., having very low Eu mass content but high fluorescent intensity, compared to many of its counterpart fluorescent materials based on Eu/ligand complex. The ultraviolet absorbance or the fluorescent intensity of the Eu-BC fluorescent paper increased with the increase of Eu-BC content but remained little changed after Eu-BC content was higher than 5%, which was due to excessive Eu-BC complexes stacking with each other without effective irritation by ultraviolet light. After 200 times of folding, the fluorescence intensity of fluorescent paper decreased by only 0.7%, which suggested that the Eu-BC fluorescent paper has very good stability and durability. The high durability of the fluorescent paper can be attributed to the nanoporous or reticular structure of BC stabilizing the Eu particles.