Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications
by
Sharad Bhatnagar 1, Toshiro Kobori 1,2, Deepak Ganesh 1,2, Kazuyoshi Ogawa 1,3 and Hideki Aoyagi 1,3,4,*
Sharad Bhatnagar 1, Toshiro Kobori 1,2, Deepak Ganesh 1,2, Kazuyoshi Ogawa 1,3 and Hideki Aoyagi 1,3,4,*
1
Life Science and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1, Tennodai, Tsukuba 305-8572, Ibaraki, Japan
2
Food Research Institute, National Agriculture and Food Research Organization, 2-1-2, Kannondai, Tsukuba 305-8642, Ibaraki, Japan
3
Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1, Tennodai, Tsukuba 305-8572, Ibaraki, Japan
4
Microbiology Research Center for Sustainability (MiCS), University of Tsukuba, 1-1-1, Tennodai, Tsukuba 305-8572, Ibaraki, Japan
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(7), 1042; https://doi.org/10.3390/nano9071042
Received: 14 June 2019 / Revised: 18 July 2019 / Accepted: 18 July 2019 / Published: 21 July 2019
(This article belongs to the Special Issue Advanced Biocompatible Nanomaterials)
In recent years, green syntheses have been researched comprehensively to develop inexpensive and eco-friendly approaches for the generation of nanoparticles. In this context, plant and microbial sources are being examined to discover potential reducing agents. This study aims to utilize an extracellular pigment produced by Talaromyces purpurogenus as a prospective reducing agent to synthesize silver nanoparticles (AgNPs). Biosynthesized AgNPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), electron probe micro analyser (EPMA), and zeta potential. The pigment functional groups involved in the generation of AgNPs were investigated using Fourier transform infrared spectroscopy. TEM images showed that the generated nanoparticles were spherical, hexagonal, rod-shaped, and triangular-shaped with a particle size distribution from 4 to 41 nm and exhibited a surface plasmon resonance at around 410 nm. DLS and zeta potential studies revealed that the particles were polydispersed and stable (−24.8 mV). EPMA confirmed the presence of elemental silver in the samples. Biosynthesized AgNPs exhibited minimum inhibitory concentrations of 32 and 4 μg/mL against E. coli and S. epidermidis, respectively. Further, cytotoxicity of the AgNPs was investigated against human cervical cancer (HeLa), human liver cancer (HepG2), and human embryonic kidney (HEK-293) cell lines using 5-fluorouracil as a positive control. A significant activity was recorded against HepG2 cell line with a half-maximal inhibitory concentration of 11.1 μg/mL.
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Keywords:
Talaromyces purpurogenus; silver nanoparticles; anti-cancer activity; anti-microbial activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style
Bhatnagar, S.; Kobori, T.; Ganesh, D.; Ogawa, K.; Aoyagi, H. Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications. Nanomaterials 2019, 9, 1042.
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