Boron Nitride Doped Polyhydroxyalkanoate/Chitosan Nanocomposite for Antibacterial and Biological Applications

The present research focused on the fabrication of biocompatible polyhydroxyalkanoate, chitosan, and hexagonal boron nitride incorporated (PHA/Ch-hBN) nanocomposites through a simple solvent casting technique. The fabricated nanocomposites were comprehensively characterized by Fourier transform infrared spectroscope (FT-IR), field emission scanning electroscope (FESEM), and elemental mapping and thermogravimetric analysis (TGA). The antibacterial activity of nanocomposites were investigated through time-kill method against multi drug resistant (MDR) microbes such as methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli) K1 strains. In addition, nanocomposites have examined for their host cytotoxicity abilities using a Lactate dehydrogenase (LDH) assay against spontaneously immortalized human keratinocytes (HaCaT) cell lines. The results demonstrated highly significant antibacterial activity against MDR organisms and also significant cell viability as compared to the positive control. The fabricated PHA/Ch-hBN nanocomposite demonstrated effective antimicrobial and biocompatibility properties that would feasibly suit antibacterial and biomedical applications.


Introduction
In recent years, with the rapid development in biomedical field, many diseases have been discovered, and many have evolved due to various changes in the biological environment. Microbial infections are the key source of chronic diseases and mortality. There are few multi-drug resistant microbes, namely E. coli and S. aureus, which are generally responsible for infections such as wound in soft tissues, skin, and bloodstream diseases [1,2]. Antibiotics are the ideal treatment for bacterial in order to feasibly enhance composite properties such as surface morphology, contact angle length, and degradability [28].
The purpose of the present research is to evaluate the potential antibacterial effect and cell viability efficacy of fabricated PHA/Ch-hBN nanocomposites that were loaded with three different concentrations of hBN nanoparticles. Therefore, to achieve this purpose, the antibacterial activity of nanocomposites were analyzed against multi-drug resistant E. coli K1 and methicillin-resistant Staphylococcus aureus (MRSA). In addition, the cell cytotoxicity assays have also been examined through the LDH method. Additionally, in the present study nontoxic acetic acid was used to dissolve the PHA instead of a standard solvent chloroform to dissolve PHA [30].

Materials and Methods
Poly 3-hydroxybuterate-co-12mol% hydroxyhexanoate (P3HB-co-12mol%HHx) powder (350,000 Da) was provided by the KANEKA corporation, Osaka Japan. Chitosan powder of a medium molecular weight was obtained from Sigma-Aldrich, St. Louis, MO, USA. Hexagonal Boron Nitride (hBN) powder with an average particle size of 70 nm was procured from Lower Friction Company (Ontario, Canada). All the analytical grade reagents were used throughout the experiment. For antibacterial assays, stationary phase bacterial strains of methicillin-resistant Staphylococcus aureus (MRSA) and E. coli (K1) were used. Moreover, spontaneously immortalized human keratinocytes (HaCaT) cell lines were used for lactate dehydrogenase assay. A cytotoxicity detection kit was purchased from Roche Diagnostics, Indianapolis, IN, USA.

Preparation of Precursor Solution
A polymer solution with a concentration of 1 mg/mL of PHA in glacial acetic acid was prepared using a standard reflux setup. In a round bottom flask, PHA powder was mixed with glacial acetic acid and stirred for 5 min (300 rpm) at 118 • C on a magnetic stirrer. The solution was then allowed to cool down at room temperature. Simultaneously, a chitosan solution with a concentration of 1 mg/mL was prepared in 1% acetic acid, which was stirred for 24 h at room temperature on a magnetic stirrer. Furthermore, hBN nanoparticles with different weight percentages with respect to PHA (0.1, 0.5, and 1) were mixed in PHA solutions using a magnetic stirrer followed by ultra-sonication to dispersed the nanoparticles. The prepared chitosan solution (1 mg/mL) was added dropwise into the hBN-doped PHA solution to obtain a 10:1 ratio of PHA/Ch. The resultant PHA/Ch-hBN solution was ultra-sonicated for 30 min to distribute nanoparticles evenly within the polymer matrix. In this experiment, three nanocomposites were prepared with different concentrations of hBN nanoparticles.

Solvent Casting
A nanocomposite film was prepared from the polymer solution as illustrated in Figure 1. The solvent casting stage was prepared using a flat glass plate while the film size and thickness were controlled by standard microbiological glass slides (75 mm × 25 mm × 1 mm). A polymer solution was dispensed carefully onto the preheated (80 • C) glass platform with complete evaporation of solvent leading to the formation of uniformly thin film. PHA/Ch and PHA films were also prepared using a similar method. The resultant nanocomposites were dried at room temperature to remove any further residuals solvent.

Antibacterial Assays
Antibacterial assays were performed to determine the percent bactericidal activity of different nanocomposites against multi-drug resistant bacteria. Briefly, 1 × 10 6 bacterial cells were incubated with PHA, PHA/Ch and PHA/Ch-hBN nanocomposites at 37 °C for 0, 2, 4, 6, and 24 h using the time-kill method. This method could be appropriate for the interaction between antibacterial agent and cultural broth. The clinical and laboratory standards institute (CLSI) has described this method as a well standardized technique to use for bacteria that is documented in M26-A of CLSI [31]. Bacterial cultures of MDR E. coli K1 and MRSA were grown over night, and the density of c. 1 × 10 8 CFU/mL was obtained through measuring 0.22 optical density (OD) at 595 nm [32]. About 10 μL, which is equal to c. 1 × 10 6 CFU/mL, was added in 100 μL nutrient broth containing the test samples of size 0.6 mm. The phosphate buffer saline was used to mark the total volume up to 200 μL. Next, cultures were ten-fold serially diluted to 10 μL, were plated on nutrient agar plates, and were incubated over night at 37 °C. For controls, bacteria incubated with gentamicin (100 μg/mL) as a positive, and PBS and PHA including PHA/Ch were used as negative control. The experiment was performed and analysed in triplicate.

Cytotoxicity Assays
HaCaT cell lines were grown in RPMI-1640 medium and supplemented with 10% (v/v) foetal bovine serum (FBS), 1% L-glutamine, 1% Penicillin-Streptomycin, and 1% minimal essential media nonessential amino acid (Life Technologies, California, CA, USA). The HaCaT cells were been seeded into the non-pyrogenic 24 well plate with the density of 5 × 10 3 per well and incubated at 37 °C with 5% CO2, including 95% humidity, for 48 h. Briefly, a HaCaT cells monolayer was incubated with nanocomposites in a RPMI-1640 medium for 24 h at 37 °C in the presence of 5% CO2 and 95% humidity. After this incubation, supernatant was aspirated to detect the cytotoxicity through measuring the release of lactate dehydrogenase (LDH), a schematic presentation of which is shown in Figure 2. Generally, the results of cytotoxicity were calculated applying the below formula: % cytotoxicity sample value control value total LDH release control value 100 Whereas, to obtained the total LDH release, HaCaT cells were treated with 0.1% Triton X-100 at 37 °C for 30 min. Similarly, the cells incubated in RPMI-1640 were used as control values. In brief, the working principle of the assay was that LDH acted as catalyst for transforming lactate to pyruvate, thereby producing NADH and H + . In next step, catalyst diaphorase converted H and H + to tetrazolium salt that was then reduced to formazan dye. The relationship between metabolically active cells and formazan dye color provided precise quantification (cell death or proliferation) from

Antibacterial Assays
Antibacterial assays were performed to determine the percent bactericidal activity of different nanocomposites against multi-drug resistant bacteria. Briefly, 1 × 10 6 bacterial cells were incubated with PHA, PHA/Ch and PHA/Ch-hBN nanocomposites at 37 • C for 0, 2, 4, 6, and 24 h using the time-kill method. This method could be appropriate for the interaction between antibacterial agent and cultural broth. The clinical and laboratory standards institute (CLSI) has described this method as a well standardized technique to use for bacteria that is documented in M26-A of CLSI [31]. Bacterial cultures of MDR E. coli K1 and MRSA were grown over night, and the density of c. 1 × 10 8 CFU/mL was obtained through measuring 0.22 optical density (OD) at 595 nm [32]. About 10 µL, which is equal to c. 1 × 10 6 CFU/mL, was added in 100 µL nutrient broth containing the test samples of size 0.6 mm. The phosphate buffer saline was used to mark the total volume up to 200 µL. Next, cultures were ten-fold serially diluted to 10 µL, were plated on nutrient agar plates, and were incubated over night at 37 • C. For controls, bacteria incubated with gentamicin (100 µg/mL) as a positive, and PBS and PHA including PHA/Ch were used as negative control. The experiment was performed and analysed in triplicate.

Cytotoxicity Assays
HaCaT cell lines were grown in RPMI-1640 medium and supplemented with 10% (v/v) foetal bovine serum (FBS), 1% L-glutamine, 1% Penicillin-Streptomycin, and 1% minimal essential media nonessential amino acid (Life Technologies, California, CA, USA). The HaCaT cells were been seeded into the non-pyrogenic 24 well plate with the density of 5 × 10 3 per well and incubated at 37 • C with 5% CO 2 , including 95% humidity, for 48 h. Briefly, a HaCaT cells monolayer was incubated with nanocomposites in a RPMI-1640 medium for 24 h at 37 • C in the presence of 5% CO 2 and 95% humidity. After this incubation, supernatant was aspirated to detect the cytotoxicity through measuring the release of lactate dehydrogenase (LDH), a schematic presentation of which is shown in Figure 2. Generally, the results of cytotoxicity were calculated applying the below formula: Whereas, to obtained the total LDH release, HaCaT cells were treated with 0.1% Triton X-100 at 37 • C for 30 min. Similarly, the cells incubated in RPMI-1640 were used as control values. In brief, the working principle of the assay was that LDH acted as catalyst for transforming lactate to pyruvate, thereby producing NADH and H + . In next step, catalyst diaphorase converted H and H + to tetrazolium salt that was then reduced to formazan dye. The relationship between metabolically active cells and formazan dye color provided precise quantification (cell death or proliferation) from the resultant supernatant. A dense purple color feasibly demonstrated the high enzyme activity due to the higher viable cells, and decrease in purple color signified the cytotoxicity.

Morphological Analysis
A surface morphological analysis of the hBN nanosheets, chitosan, and PHA/Ch-hBN nanocomposites were comprehensively evaluated through FESEM and TEM, as shown in Figure 3. Figure 3a represents the FESEM image of hBN which has depicted the discs like morphology in nano range where the 2D hBN flakes can be seen densely stacked onto each other. In addition, the obtained TEM image further confirm the disc-like morphology of hBN nanoflakes ( Figure 3b). The stacked disc-like morphology of hBN, a characteristic of 2D, has been clearly established by FESEM and TEM analysis. The FESEM micrograph of chitosan is shown in Figure 3c, which demonstrates the cotton flakes like structures that is the characteristic morphology of chitosan [33]. Similar topology was also observed in nanocomposite depicted in Figure 3d, which is clearly revealing the formation of cross-linked polymeric networking of PHA/Ch. The morphology of the nanocomposites appears to be 2D layered type, which might be due to the interaction of polymeric chains with 2D hBN. However, from Figure 3d, it was found that the hBN nanoflakes were difficult to visualize within the nanocomposite since hBN nanodiscs were deeply embedded in the PHA polymeric matrix and difficult to visualize using FESEM imaging alone. Additionally, the amount of hBN is much less as compared to the bare polymer matrix, hence making its analysis further cumbersome through FESEM single-handedly. Therefore, the SEM elemental mapping analysis technique appears to be an appropriate method to demonstrate the presence and even distribution of hBN within the PHA/Ch matrix; this was further supported by energy dispersive X-ray EDX investigations.

Morphological Analysis
A surface morphological analysis of the hBN nanosheets, chitosan, and PHA/Ch-hBN nanocomposites were comprehensively evaluated through FESEM and TEM, as shown in Figure 3. Figure 3a represents the FESEM image of hBN which has depicted the discs like morphology in nano range where the 2D hBN flakes can be seen densely stacked onto each other. In addition, the obtained TEM image further confirm the disc-like morphology of hBN nanoflakes (Figure 3b). The stacked disc-like morphology of hBN, a characteristic of 2D, has been clearly established by FESEM and TEM analysis. The FESEM micrograph of chitosan is shown in Figure 3c, which demonstrates the cotton flakes like structures that is the characteristic morphology of chitosan [33]. Similar topology was also observed in nanocomposite depicted in Figure 3d, which is clearly revealing the formation of cross-linked polymeric networking of PHA/Ch. The morphology of the nanocomposites appears to be 2D layered type, which might be due to the interaction of polymeric chains with 2D hBN. However, from Figure 3d, it was found that the hBN nanoflakes were difficult to visualize within the nanocomposite since hBN nanodiscs were deeply embedded in the PHA polymeric matrix and difficult to visualize using FESEM imaging alone. Additionally, the amount of hBN is much less as compared to the bare polymer matrix, hence making its analysis further cumbersome through FESEM single-handedly. Therefore, the SEM elemental mapping analysis technique appears to be an appropriate method to demonstrate the presence and even distribution of hBN within the PHA/Ch matrix; this was further supported by energy dispersive X-ray EDX investigations.

FT-IR
The FT-IR spectra of PHA, Chitosan, hBN, PHA/Ch and PHA/Ch doped hBN nanocomposites are shown in Figure 5. The FT-IR spectrum of PHA is illustrated in Figure 5a. The IR peak appearing at 1700 cm −1 may be assigned to C=O (ester carbonyl) stretching of PHA polymer. The prominent bands at the spectral region of 1172 to 1280 cm −1 are associated C-O-C vibrations within the PHA polymeric chains. The IR bands appearing around at 3436 cm −1 can be assigned to -OH vibration in the carboxyl group present in copolymeric chains of PHA and P(3HB-co-3HHx). The prominent IR peak at 2933 cm −1 is the characteristic -C-H-vibrations of the methylene group present in the macromolecules of PHA (Figure 5a) [34,35]. Figure 5b exemplifies the FT-IR spectrum of chitosan. The broad IR bands at the range of 3352-3288 cm −1 may be assigned to stretching modes of the -OH and primary amine groups. The characteristic IR peaks appearing at 1640 cm −1 and 1588 cm −1 might be attributed to the C-N and C=O (amide) stretching modes. The C-H stretching and bending was ascribed to the bands at the spectral region of 2869 cm −1 and 1427 cm −1 , respectively (Figure 5b) [36]. From the hBN spectra shown in Figure 5c, the typical stretching vibration bands of B-N bond and B-N-B bending vibration were attributed around the spectral region of 1338 cm −1 and 767 cm −1 [37,38]. The IR spectrum of the various nanocomposites scaffolds are represented in Figure 5d. As obvious from the spectra of the nanocomposites, they comprise of the characteristic peaks and IR bands of PHA, chitosan, and hBN. As a result of an overlapping of the C-H stretching of chitosan in composites, the IR band at approximately 2877 cm −1 became less intense and was been slightly shifted to 2875 cm −1 . In addition, the C=O (amide) and C-N stretching bands in chitosan around at

FT-IR
The FT-IR spectra of PHA, Chitosan, hBN, PHA/Ch and PHA/Ch doped hBN nanocomposites are shown in Figure 5. The FT-IR spectrum of PHA is illustrated in Figure 5a. The IR peak appearing at 1700 cm −1 may be assigned to C=O (ester carbonyl) stretching of PHA polymer. The prominent bands at the spectral region of 1172 to 1280 cm −1 are associated C-O-C vibrations within the PHA polymeric chains. The IR bands appearing around at 3436 cm −1 can be assigned to -OH vibration in the carboxyl group present in copolymeric chains of PHA and P(3HB-co-3HHx). The prominent IR peak at 2933 cm −1 is the characteristic -C-H-vibrations of the methylene group present in the macromolecules of PHA (Figure 5a) [34,35]. Figure 5b exemplifies the FT-IR spectrum of chitosan. The broad IR bands at the range of 3352-3288 cm −1 may be assigned to stretching modes of the -OH and primary amine groups. The characteristic IR peaks appearing at 1640 cm −1 and 1588 cm −1 might be attributed to the C-N and C=O (amide) stretching modes. The C-H stretching and bending was ascribed to the bands at the spectral region of 2869 cm −1 and 1427 cm −1 , respectively (Figure 5b) [36]. From the hBN spectra shown in Figure 5c, the typical stretching vibration bands of B-N bond and B-N-B bending vibration were attributed around the spectral region of 1338 cm −1 and 767 cm −1 [37,38]. The IR spectrum of the various nanocomposites scaffolds are represented in Figure 5d. As obvious from the spectra of the nanocomposites, they comprise of the characteristic peaks and IR bands of PHA, chitosan, and hBN. As a result of an overlapping of the C-H stretching of chitosan in composites, the IR band at approximately 2877 cm −1 became less intense and was been slightly shifted to 2875 cm −1 . In addition, the C=O (amide) and C-N stretching bands in chitosan around at 1640 cm −1 and 1588 cm −1 , respectively, vanished in composites. The absorption bands at 2930 cm −1 and 1450 cm −1 in bare PHA and chitosan showed slight shifts in the composites. The characteristic peaks at 1338 cm −1 and 767 cm −1 in hBN were detected at 1379 cm −1 and 738 cm −1 in hBN doped PHA/Ch nanocomposites. However, many IR peaks and bands have depicted significant changes shift as compared to the spectrum of of bare PHA, chitosan, and hBN signifying, the chemical associations or interactions with each other. Thus, the FT-IR spectrum suggests the formation of a PHA/Ch-hBN nanocomposite, which is in good agreement with the earlier analysis. PHA/Ch nanocomposites. However, many IR peaks and bands have depicted significant changes shift as compared to the spectrum of of bare PHA, chitosan, and hBN signifying, the chemical associations or interactions with each other. Thus, the FT-IR spectrum suggests the formation of a PHA/Ch-hBN nanocomposite, which is in good agreement with the earlier analysis.  Figure 6 represented the TGA results of PHA/Ch and PHA/Ch-hBN nanocomposites including bare PHA, chitosan, and hBN. The TGA analysis was performed under a nitrogen atmosphere at a temperature range of 30-500 °C for 10 °C/min. As apparent from the TGA thermogram of hBN, no weight loss pccured until 500 °C, which indicated a higher level of thermal stability. On the other hand, chitosan has revealed a 10% weight loss between 50-115 °C, which can be attributed to the loss of residual water and adsorbed moisture. The major weight loss in chitosan is observed at 250-343 °C, which may be assigned to the decomposition of ethereal groups, glucosamine moieties, and ring opening reactions. The thermogram of PHA reveals that it is thermally stable until 200 °C, the gradual weight loss has initiated around 250 °C, and a considerable weight loss of 98.6% was noted form 268-310 °C. This thermal degradation of PHA may be due to the degradation of unreacted free monomers and the decomposition of hydrocarbon polymeric chains. The blend of PHA/Ch has shown less thermal stability as bare compared to PHA but higher stability as compared to chitosan, which indicates some interactions and crosslinking between the two polymers. Though PHA has shown 100% decomposition till 500 °C, PHA/Ch has revealed 4% residue at 500 °C, thus signifying some synergistic associations between tow biopolymers imparting thermal stability. Finally, the PHA/Ch-hBN nanocomposite revealed the improved thermal stability compared to PHA/Ch composite, which is possibly due to the addition more thermally stable hBN nanomaterial. Therefore, from the analysis, it was shown that the thermal stability of PHA/Ch-hBN  Figure 6 represented the TGA results of PHA/Ch and PHA/Ch-hBN nanocomposites including bare PHA, chitosan, and hBN. The TGA analysis was performed under a nitrogen atmosphere at a temperature range of 30-500 • C for 10 • C/min. As apparent from the TGA thermogram of hBN, no weight loss pccured until 500 • C, which indicated a higher level of thermal stability. On the other hand, chitosan has revealed a 10% weight loss between 50-115 • C, which can be attributed to the loss of residual water and adsorbed moisture. The major weight loss in chitosan is observed at 250-343 • C, which may be assigned to the decomposition of ethereal groups, glucosamine moieties, and ring opening reactions. The thermogram of PHA reveals that it is thermally stable until 200 • C, the gradual weight loss has initiated around 250 • C, and a considerable weight loss of 98.6% was noted form 268-310 • C. This thermal degradation of PHA may be due to the degradation of unreacted free monomers and the decomposition of hydrocarbon polymeric chains. The blend of PHA/Ch has shown less thermal stability as bare compared to PHA but higher stability as compared to chitosan, which indicates some interactions and crosslinking between the two polymers. Though PHA has shown 100% decomposition till 500 • C, PHA/Ch has revealed 4% residue at 500 • C, thus signifying some synergistic associations between tow biopolymers imparting thermal stability. Finally, the PHA/Ch-hBN nanocomposite revealed the improved thermal stability compared to PHA/Ch composite, which is possibly due to the addition more thermally stable hBN nanomaterial. Therefore, from the analysis, it was shown that the thermal stability of PHA/Ch-hBN nanocomposite has been improved as compared to PHA/Ch and can be applied in applications which require higher temperature (Up to 250 • C). Nanomaterials 2019, 9, x FOR PEER REVIEW 9 of 14 nanocomposite has been improved as compared to PHA/Ch and can be applied in applications which require higher temperature (Up to 250 °C).

Antibacterial Analysis
The antibacterial activities of fabricated nanocomposites were tested against MDR E. coli K1 and MRSA through the time-kill method. The results of PHA/Ch-hBN (hBN 0.1, 0.5 and 1.0 wt% wrt PHA) exhibited significant bactericidal activity against E. coli K1 (p < 0.05), which is represented in Figure 7a and Table 1. When tested against MRSA, the results revealed that nanocomposites significantly reduced the growth of bacteria (p-value) (<0.05) (Figure 7b and Table.1). The hBN nanocomposites remarkably reduce the percent viability of bacteria as compared to negative control (Figure 7a,b).

Antibacterial Analysis
The antibacterial activities of fabricated nanocomposites were tested against MDR E. coli K1 and MRSA through the time-kill method. The results of PHA/Ch-hBN (hBN 0.1, 0.5 and 1.0 wt% wrt PHA) exhibited significant bactericidal activity against E. coli K1 (p < 0.05), which is represented in Figure 7a and Table 1. When tested against MRSA, the results revealed that nanocomposites significantly reduced the growth of bacteria (p-value) (<0.05) (Figure 7b and Table 1). The hBN nanocomposites remarkably reduce the percent viability of bacteria as compared to negative control (Figure 7a,b). In brief, the antibacterial activity was assessed at different time intervals, and the first 2, 4, and 6 h of time points demonstrated effective bactericidal activity as compared to negative control (PHA/Ch), including 24 hours' time point. From the graph, it is hypothesized that the bactericidal activity was time dependent in this experiment, which was subjected to the morphology of nanocomposites. Furthermore, an increase in the concentration of hBN nanomaterial with the PHA/Ch composition has revealed the improved bactericidal ability. However, all the three concentrations of hBN nanocomposites have shown a significant reduction of E. coli K1 and MRSA for up to 24 h. The Gentamicin was applied as positive control, which killed 100% of the bacterial colonies. The statistical p-values were defined by applying two sample T-test distribution, while (*) is <0.05; (**) is <0.01; and (***) is <0.001, respectively. In brief, the antibacterial activity was assessed at different time intervals, and the first 2, 4, and 6 h of time points demonstrated effective bactericidal activity as compared to negative control (PHA/Ch), including 24 hours' time point. From the graph, it is hypothesized that the bactericidal activity was time dependent in this experiment, which was subjected to the morphology of nanocomposites. Furthermore, an increase in the concentration of hBN nanomaterial with the PHA/Ch composition has revealed the improved bactericidal ability. However, all the three concentrations of hBN nanocomposites have shown a significant reduction of E. coli K1 and MRSA for up to 24 h. The Gentamicin was applied as positive control, which killed 100% of the bacterial colonies. The statistical p-values were defined by applying two sample T-test distribution, while (*) is <0.05; (**) is <0.01; and (***) is <0.001, respectively.

Cell Cytotoxicity Assay against HaCaT Cell Lines
For the biomedical applications, cell viability testing is an important step to determine the cellular effects of external toxicants and the biosafety of biomaterials. Therefore, the synthesized PHA/Ch-hBN nanocomposites were tested for their cytotoxic effects against HaCaT cell lines, and the cell viability was quantified through LDH assays. HaCaT cells have treated with test samples for up to 24 h, and the resultant cells have visualized by inverted microscope at 200X lens, which is presented in Figure 8.

Cell Cytotoxicity Assay against HaCaT Cell Lines
For the biomedical applications, cell viability testing is an important step to determine the cellular effects of external toxicants and the biosafety of biomaterials. Therefore, the synthesized PHA/Ch-hBN nanocomposites were tested for their cytotoxic effects against HaCaT cell lines, and the cell viability was quantified through LDH assays. HaCaT cells have treated with test samples for up to 24 h, and the resultant cells have visualized by inverted microscope at 200X lens, which is presented in Figure 8.
The microscopic images have shown clearly that there was no decrease of confluent monolayer, which was treated with negative control (Figure 8a) and possessed complete destruction with a positive control, as depicted in Figure 8b (1% Trixton-100). Whereas both concentraions of the PHA/Ch-hBN (1.0% hBN wrt PHA) nanocomposite showed no cytotoxic effects against the HaCaT cell lines as shown in Figure 8c. Figure 9 illustrates the comprehensive percent cell cytotoxicity data. The microscopic images have shown clearly that there was no decrease of confluent monolayer, which was treated with negative control (Figure 8a) and possessed complete destruction with a positive control, as depicted in Figure 8b (1% Trixton-100). Whereas both concentraions of the PHA/Ch-hBN (1.0% hBN wrt PHA) nanocomposite showed no cytotoxic effects against the HaCaT cell lines as shown in Figure 8c. Figure 9 illustrates the comprehensive percent cell cytotoxicity data.

Comparison of Antibacterial Efficiencies
The antibacterial performance of the synthesized h-BN doped nanocomposite was compared with similar reported studies as illustrated in Table 2. In this investigation, the first two hours bactericidal activity of hBN was found to be 92% and 97% against E. coli K1 and MRSA, respectively, which is comparatively much enhanced as compared with other reports. As is apparent from the comparative data shown in Table 2, the synthesized hBN doped nanocomposite  The microscopic images have shown clearly that there was no decrease of confluent monolayer, which was treated with negative control (Figure 8a) and possessed complete destruction with a positive control, as depicted in Figure 8b (1% Trixton-100). Whereas both concentraions of the PHA/Ch-hBN (1.0% hBN wrt PHA) nanocomposite showed no cytotoxic effects against the HaCaT cell lines as shown in Figure 8c. Figure 9 illustrates the comprehensive percent cell cytotoxicity data.

Comparison of Antibacterial Efficiencies
The antibacterial performance of the synthesized h-BN doped nanocomposite was compared with similar reported studies as illustrated in Table 2. In this investigation, the first two hours bactericidal activity of hBN was found to be 92% and 97% against E. coli K1 and MRSA, respectively, which is comparatively much enhanced as compared with other reports. As is apparent from the comparative data shown in Table 2, the synthesized hBN doped nanocomposite

Comparison of Antibacterial Efficiencies
The antibacterial performance of the synthesized h-BN doped nanocomposite was compared with similar reported studies as illustrated in Table 2. In this investigation, the first two hours bactericidal activity of hBN was found to be 92% and 97% against E. coli K1 and MRSA, respectively, which is comparatively much enhanced as compared with other reports. As is apparent from the comparative data shown in Table 2, the synthesized hBN doped nanocomposite offers enhanced antibacterial efficiency and can thus be applicable for efficient in antibacterial treatments and biological applications.

Conclusions
An PHA/Ch matrix and hBN nanodiscs-doped nanocomposite scaffolds have been efficaciously designed and fabricated through a facile solvent casting technique. hBN nanodiscs were effectively amalgamated within the inter-cross linked polymeric network of the PHA/Ch. The current examination indicated the synergistic consequence of the thermally stable hBN and the biopolymers, thus leading to the augmentation of the antibacterial activity of the nanocomposite against MDR E. coli K1 and MRSA. The fabricated polymeric scaffolds have exhibited superior bactericidal activity and killed pathogenic bacteria competently in a small time interval. Besides, nanocomposite has good cytocompatibility with human cells, as there was a minimal cytotoxicity observed. In conclusion, the multifunctional PHA/Ch-hBN nanocomposites have exhibited broad spectrum antibacterial efficiency and cytocompatibility within appropriate concentrations. Therefore, the synthesized nanocomposites can be considered as safe, efficient, and stable antibacterial scaffolds for biological applications.