Iron Oxide Nanocrystals for Magnetic Hyperthermia Applications

Magnetic nanocrystals have been investigated extensively in the past several years for several potential applications, such as information technology, MRI contrast agents, and for drug conjugation and delivery. A specific property of interest in biomedicine is magnetic hyperthermia—an increase in temperature resulting from the thermal energy released by magnetic nanocrystals in an external alternating magnetic field. Iron oxide nanocrystals of various sizes and morphologies were synthesized and tested for specific losses (heating power) using frequencies of 111.1 kHz and 629.2 kHz, and corresponding magnetic field strengths of 9 and 25 mT. Polymorphous nanocrystals as well as spherical nanocrystals and nanowires in paramagnetic to ferromagnetic size range exhibited good heating power. A remarkable 30 °C temperature increase was observed in a nanowire sample at 111 kHz and magnetic field of 25 mT (19.6 kA/m), which is very close to the typical values of 100 kHz and 20 mT used in medical treatments.


Introduction
Iron oxide nanocrystals are ideal materials for biomedical applications, due to their well-established biocompatibility [1] and inherent multifunctionality [2]. For example, iron oxide nanoparticles have received FDA approval for use in humans in magnetic resonance imaging (MRI) as contrast agents [3,4]. They have also been tested in Phase 1 and Phase 2 trials for hyperthermic antitumor therapy in Germany, and seem to exhibit no systemic toxicity [5,6]. This application exploits the ability of magnetic nanoparticles to release thermal energy upon placement in an external oscillating magnetic field (magnetic hyperthermia) [7,8]. In addition, magnetic nanoparticles hold potential as efficient drug carriers [9,10], since they may be guided by the magnetic field toward a specific area of interest, thereby reducing the required dose and eliminating systemic side effects.
In the frequency range suitable for human patient treatment, three potential mechanisms are implicated in heating: Néel relaxation, Brownian motion relaxation, and hysteresis losses in the ferroferrimagnetic size range. This phenomenon is exploited in the application of hyperthermic tumor destruction, or thermotherapy. Furthermore, thermal energy from magnetic hyperthermia can potentially be used to trigger drug release from the nanocrystals, once the magnetic nanocrystals have reached the area of interest.
In this paper, we report on the synthesis and characterization of iron oxide nanocrystals capped with polyethylene glycol (PEG) to enhance solubility of nanocrystals in water and reduce their oxidation and aggregation. Iron oxide nanocrystals of various sizes and morphologies were synthesized and characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDS), and tested for magnetic hyperthermia using the Magnetherm system manufactured by NanoTherics, Ltd.

Synthesis of Colloidal Nanocrystals
Iron oxide nanocrystals were synthesized in a high boiling point solvent consisting of inert hydrocarbons and capped with PEG. For the synthesis we used a modified procedure published elsewhere [11]. Our modifications to it yielded nanocrystals of various sizes and morphologies. Polymorphous nanocrystals as well as spherical nanocrystals and nanowires were obtained by varying the temperature of the synthesis through the use of an appropriate solvent. For hyperthermia experiments, nanocrystals in the superparamagnetic to ferromagnetic size range of ~20 nm were chosen. c)

Structural Characterization
The TEM images in Figures 1-2 show the various morphologies and sizes of Fe 3 O 4 nanocrystals we were able to obtain prior to PEG capping. Polymorphous nanocrystals shown in Figure 1a were obtained with eicosane solvent (342.7 °C boiling point). Monodisperse spheres with a diameter of ~ 30 nm in Figure 1b formed from polymorphous nanocrystals shown in Figure 1a) when the reaction mixture was allowed to cool for 30 min before being refluxed again. Spheres of ~22 nm in diameter (Figure 1c and nanowires 55 × 2 nm (Figure 1d) were made in n-docosane (boiling point 370 °C) and n-dodecane (boiling point 216.2 °C), respectively. We performed high-resolution TEM to confirm high crystallinity of the nanocrystals. Image in Figure 2 represents fringes observed for the monodisperse spheres from Figure 1b.

Magnetic Characterization
A typical feature in magnetic nanocrystals is their irreversible ferromagnetic behavior below the blocking temperature T B and reversible magnetization above it, caused by superparamagnetic behavior of the nanocrystals. The blocking temperature can be found experimentally by measuring magnetization under field-cooling (FC) and zero-field cooling (ZFC) conditions. Below T B , the Néel relaxation time τ N is larger than the measurement time τ m (typically 100 s), and magnetization depends strongly on the field history. Above T B , magnetization is strongly affected by thermal fluctuations (τ m > τ N ), making FC and ZFC curves coincide. In other words, for a given measurement time τ m , hysteretic behavior observed below T B would not be observed above T B . We measured temperature dependence of magnetization for the Fe 3 O 4 nanocrystal samples under ZFC and FC conditions. The dc (τ m = 100 s) magnetization of the ferrofluid samples was measured with a dc field of 100 Oe in the temperature range between 9 K and 350 K.
In the entire temperature range up to 350 K, the Fe 3 O 4 nanocrystal samples demonstrated strong ferromagnetic behavior as evidenced by the gap between the ZFC and FC curves persisting even at 350 K ( Figure 5). From the ZFC curve, we can loosely estimate T B to be ~175 K, but even above that temperature equilibrium magnetization of the nanocrystal sample was not reached, and superparamagnetic behavior of the nanocrystals was not observed.
Strong ferromagnetic behavior of the Fe 3 O 4 nanocrystal samples was confirmed in magnetic hysteresis measurements. Consistent with the results of dc magnetization measurements, magnetic hysteresis measurements at 293 K performed on Fe 3 O 4 polymorphous nanocrystals ( Figure 6a) find large coercivity ~37 mT (~29 kA/m) at 100 s measurement time. Even larger coercivity of ~119 mT (~94.7 kA/m) was measured for ~22 nm Fe 3 O 4 nanospheres.

Magnetic Hyperthermia Experiments
Magnetic hyperthermia for the Fe 3 O 4 nanocrystal samples was tested using a NanoTherics, Ltd. Magnetherm that operates at frequencies between 100 and 1000 kHz. Samples were prepared as described in the Experimental Section and dispersed in deionized water. All concentrations were 30 mg/mL and sample volumes were 5 mL. The nanocrystals compared in the following graphs were 22 nm spheres, polymorphous nanocrystals, and 55 × 2 nm wires. Heating of the nanocrystals was tested at frequencies of 111.1 kHz and 629.2 kHz. Data acquisition for hyperthermia was started at ambient temperature. Temperature increase was measured using an optical thermometer with an accuracy of ±0.5 °C.   Figure 7b shows the heating of the same nanocrystals at a frequency of 629.2 kHz (magnetic field of 9 mT). The spherical and polymorphous particles follow a similar trend consistent with their similar morphology and particle volume. Although they do heat at the frequency of 111.1 kHz, the observed heating was relatively low. Interestingly, the total increase in temperature after 40 min was 6 °C for spheres, whereas, it was only 1 °C for polymorphous nanocrystals. When the frequency of the oscillating magnetic field was increased to 629.2 kHz, the spheres and polymorphous nanocrystals showed increased heating. But, unlike the data obtained at 111.1 kHz, the total temperature increase was similar for the polymorphous nanocrystals and nanocrystals of spherical shape: 16 °C for spheres and 15 °C for polymorphous nanocrystals. As for the nanowires, the observed trend was just the opposite. The total temperature increase at 111.1 kHz after 40 min was remarkable 30 °C and, notably, saturation of the temperature was not reached in this timeframe. At 629.2 kHz, however, the increase of temperature generated by the wires was much less than the one obtained by spheres and polymorphous nanocrystals, representing the total temperature increase of 4 °C.
It has been shown that the transition from ferromagnetic to superparamagnetic behavior is associated with the change of the loss mechanism and, accordingly, of the heating effect of magnetic nanocrystals in hyperthermia experiments [12]. Hysteresis losses dominate in ferromagnetic nanocrystals, whereas heat production in superparamagnetic ones is due to relaxation losses. Since the blocking temperature T B explicitly depends on the measurement time τ m (inversely proportional to the frequency of the oscillating magnetic field), superparamagnetic nanocrystals, as measured in dc magnetization experiments, become ferromagnetic at sufficiently high frequencies > 1/τ N (or > 1/τ, where τ = τ Ν τ Β /(τ Ν + τ Β ), if both Néel and Brownian relaxation mechanism are present) and generate heat due to hysteresis losses. With the Fe 3 O 4 nanocrystal samples demonstrating strong ferromagnetic behavior in dc magnetization and hysteresis measurements, hysteresis losses are expected to be the main mechanism of heating in the operating frequency range of our hyperthermia experiments. We estimated the Néel relaxation time τ N at room temperature for the Fe 3 O 4 polymorphous nanocrystals ( Figure 5) as follows: τ N = τ 0 exp(E b /k B T), where E b is the magnetic anisotropy energy barrier, k B is the Boltzmann constant, and τ 0 = 10 −10 s is the attempt time. E b is related to the blocking temperature T B as E b = k B T B ln(τ m /τ 0 ) = 27.6 k B T B , and we arrive at τ N = τ 0 exp(27.6 T B /T) for the Néel relaxation time. At T = 300 K, τ N ≈ 0.001 s. At the frequencies of interest ω >> 1/τ N , that is far from the relaxation resonance, the Néel relaxation losses saturate at a level that is negligible for large enough τ N [12]. Therefore, we will interpret our results based on the mechanism of hysteresis losses prevailing.
When hysteresis losses are the main heating factor, heating power is proportional to the area of the hysteresis loop and to the frequency of the applied magnetic field. Approximately 6-fold increase in the heating power is expected when the frequency is changed from 111.1 kHz to 629.2 kHz. The observed increase in heating power from the Fe 3 O 4 polymorphous nanocrystals and nanospheres is not that large. We note, however, that both the frequency and amplitude of the magnetic field were changed in our experiments, and the magnetic field strength was reduced from 25 mT at 111.1 kHz to 9 mT at 629.2 kHz, which can explain the heating power increase not being proportional to the frequency for the Fe 3 O 4 polymorphous nanocrystals and nanospheres. The higher temperature increase of 6 °C for the spheres compared to 1 °C for the polymorphous nanocrystals at 111.1 kHz can be explained by a significantly larger area of their hysteresis. However, the difference in the specific heat production between the spherical and polymorphous nanocrystals at 629.2 kHz is not that pronounced.
We consider hyperthermia experiments with nanowires separately, as their morphology differs dramatically from that of polymorphous nanocrystals and nanospheres, and may be the decisive factor. Fine magnetite particles of needle shape with high aspect ratio have been investigated previously by Hergt et al. [12]. High potential for hyperthermia was noted there for such particles that possess very high shape anisotropy, and hence high-energy barrier for remagnetization, resulting in a wide hysteresis and high hysteresis losses. It was concluded, however, that strong magnetic fields, very often unacceptable for human patients, are required to fully utilize their potential. Very strong nonlinear dependence of the hysteresis loss on the strength of the applied magnetic field was reported. Comparison was made among particles of different shape, and it was found that needles were by far superior when applied magnetic field exceeded ~35 kA/m, while below that value the magnetic field was not strong enough to open the hysteresis loop in needles, and their hysteresis losses were by several orders of magnitude lower compared to particles of other shapes with low aspect ratio. We expect similar effects to be observed in nanowires that are characterized with even higher aspect ratios of their shape. We believe that our results for hyperthermia in nanowires can be explained by similar superlinear dependence of their hysteresis loss on the magnetic field strength, with that superlinear dependence being much stronger than mere proportionality of the heating power to the frequency of the applied magnetic field.
We note that the remarkable 30 °C temperature increase was observed in nanowires sample at 111 kHz and magnetic field of 25 mT (19.6 kA/m), which is very close to the typical values used in medical treatments −100 kHz and 20 mT [13,14].
In our analysis, we have not considered one more possible mechanism of heat generation during hyperthermia, namely, Brownian relaxation that can be significant in particles of that size. Additional experiments are needed to clarify contribution of Brownian relaxation to the observed heat production in hyperthermia experiments.
For structural characterization, TEM/EDS samples were prepared by placing a drop of the colloidal solution onto a 200-mesh carbon-coated copper grid. The solvent was allowed to evaporate away, thus fixing the sample on the grid. The JEOL-2010F transmission electron microscope was equipped with an OXFORD Link ISIS energy dispersive spectroscopy (EDS) apparatus, which determined elemental composition. The electron beam was focused on a single nanocrystal and the characteristic X-ray peaks specific to each element were identified using the OXFORD Link ISIS software. The iron oxide phase and crystal structure was determined using a Rigaku Smartlab ® X-Ray Diffractometer (XRD) with a Cu K α source (0.154 nm).
The dc (τ m = 100 s) magnetization of the ferrofluid samples was measured with a dc field of 100 Oe in the temperature range between 9 K and 350 K using a Quantum Design magnetic property measurement system (MPMS) superconducting quantum interference device (SQUID) magnetometer.

Synthesis of Iron Oleate Precursor Complex
The procedure consisted of two steps: synthesis of the iron oleate precursor complex and synthesis of the iron oxide nanocrystals. The precursor was iron oleate, (iron(II,III) [(9Z)-9-octadecenoate] n ) where n is the coordination number of iron, and could form a monomer, dimer, or trimer [15,16], produced in our laboratory using a modified procedure of Bronstein et al. [15]. The formation of the complex was verified with UV-Vis-NIR spectroscopy. The iron oleate complex was formed from the combination of sodium oleate salt (sodium (9Z)-9-octadecenoate) and iron(III)chloride hexahydrate (FeCl 3 ·6H 2 O). In a standard reaction, 6.75 g of FeCl 3 ·6H 2 O was combined with 25 mL of deionized water and vacuum-filtered through 0.22 μm filter paper. The mixture was then combined with 24.35 g of sodium oleate in a three-neck round bottom flask. 150 mL of a stock solution, consisting of a 2:4:6 mixture of deionized water, ethanol, and hexane, was added to the flask. Under argon flow, the mixture was vented and filled for three one-minute intervals, in order to remove all oxygen from the reaction flask. The solution was the slowly (5 °C/min) heated to 50 °C under vigorous stirring. Once the solid sodium oleate salt had completely melted and the reflux had begun (around 50-60 °C), the temperature was further increased (3 °C/min) to 70 °C and the flask was kept at this temperature for four hours, ensuring that the total reflux time was 4 hours. The mixture was then cooled to 60 °C and washed three times with a 1:1 mixture of hexane and deionized water in a separatory flask. The organic layer was placed in a rotary evaporator (Rotovap) with the water bath set at 30 °C until the hexane and ethanol were evaporated away. The resulting waxy complex was then dried in a vacuum oven for 24 hours. The overall reaction is illustrated in Figure 8. Wet iron oleate complex (the hydrate form) as obtained from the procedure described above was a reddish-brown, highly viscous liquid. It could be further purified by several methods to obtain dry iron oleate. Specifically, we purified it with ethanol, acetone, hexane, and water washes and dried in the oven at 70 °C for 24 hours. After drying, the product was a black solid. When dry iron oleate was used as a precursor, the ratio of oleic acid to iron oleate in the reaction mixture was increased as an additional stabilizing factor.

Synthesis of Polymorphous Iron Oxide Nanocrystals
In a standard reaction, 5 g of dry iron oleate was combined with 5.6 mL of oleic acid and 13.15 g of eicosane (boiling point 342.7 °C). The mixture was slowly heated (3 °C/min) to 50 °C under argon flow and vigorous stirring. Once the reactants had dissolved, the temperature was further increased to 342 °C at a heating rate of 3.0 °C/min. For ~20 nm polymorphous nanocrystals, the mixture was allowed to reflux for 32 min. For larger particles, the reflux time was extended, with an average growth of 2.2 nm per minute. The maximum size without adding additional reagents was 250 nm after 99 min. If the nanocrystals were allowed to cool for any amount of time before being refluxed at the same temperature again, the nanocrystal growth favored spherical morphology. After 30 min of reflux, the spheres were highly monodisperse.

Synthesis of Iron Oxide Nanowires
In a standard reaction, 5 g of wet iron oleate was combined with 1.6 mL of oleic acid and 13.15 g of n-dodecane (boiling point 216.2 °C). The mixture was slowly (3 °C/min) heated to 50 °C under argon flow and vigorous stirring. Once the reactants had dissolved, the temperature was further increased to 216 °C at a heating rate of 3 °C/min. For ~55 × 2 nm wires, the mixture was allowed to reflux for 60 min.

Synthesis of Iron Oxide Spherical Nanocrystals
In a standard reaction, 5 g of wet iron oleate was combined with 1.6 mL oleic acid and 13.15 g of n-docosane (boiling point 370 °C). The mixture was slowly (3 °C/min) heated to 50 °C under argon flow and vigorous stirring. Once the reactants had dissolved, the temperature was further increased to 370 °C at a heating rate of 3 °C/min. For ~20 nm particles, the mixture was allowed to reflux for 32 min. For larger particles, the reflux time was extended, with an average growth rate of 1.6 nm per minute. The maximum size without adding additional reagents was 150 nm after 99 min.

Polyethylene Glycol (PEG) Capping of Iron Oxide Nanocrystals
PEG capping was performed following a modified procedure from [17]. The iron oxide nanocrystals were solvated in chloroform and combined with PEG using a nanocrystal to PEG mass ratio of 1:2.

Conclusions
We have synthesized and characterized PEG-capped iron oxide nanocrystals of various morphologies in paramagnetic to ferromagnetic size range that will allow further functionalization and drug conjugation. DC magnetization and ac heating power (hyperthermia characteristics) of the PEG-capped Fe 3 O 4 nanocrystals in water have been studied. The Fe 3 O 4 nanocrystal samples demonstrated strong ferromagnetic behavior and hysteresis losses were identified as the main mechanism of heating in hyperthermia experiments. Our hyperthermia data shows that all three nanocrystal morphologies, spheres, polymorphous nanocrystals, and wires are good candidates for thermotherapy. Significant heating was observed well within the limits for oscillating magnetic field parameters established for biological applications. The observed temperature increase for Fe 3 O 4 nanospheres at 111.1 kHz and 25 mT after 40 min was 6 °C. If the corresponding temperature increase took place from normal human body temperature (36.6 °C) as a starting point, it would bring the local temperature up to 42.6 °C, which is right within the desirable temperature limits for the applications of medical hyperthermia (41-45 °C) [18]. Of special interest for hyperthermia applications are Fe 3 O 4 nanowires that demonstrated a remarkable 30 °C temperature increase under magnetic field conditions that were very close to the typical values used in medical treatments.