Ofloxacin Removal from Aqueous Media by Means of Magnetoactive Electrospun Fibrous Adsorbents

Functionalized electrospun polymer microfibrous membranes were fabricated by electrospinning and further surface-functionalized with magnetic iron oxide (FexOy) nanoparticles to yield magnetoactive nanocomposite fibrous adsorbents. The latter were characterized in respect to their morphology, mechanical properties and magnetic properties while they were further evaluated as substrates for removing Ofloxacin (OFL) from synthetic aqueous media and secondary urban wastewater (UWW) under varying physicochemical parameters, including the concentration of the pharmaceutical pollutant, the solution pH and the membranes’ magnetic content. The magnetic-functionalized fibrous adsorbents demonstrated significantly enhanced adsorption efficacy in comparison to their non-functionalized fibrous analogues while their magnetic properties enabled their magnetic recovery and regeneration.


Introduction
During recent years, there has been a tremendous increase in the accumulation of pharmaceuticals in aquatic systems, owing to the exponentially increased amounts of drugs consumed worldwide [1,2]. Antibiotics such as Tetracycline, Ciprofloxacin, Levofloxacin, Amoxicillin, Norfloxacin, Ofloxacin, etc., predominate as organic pharmaceutical water contaminants [3]. Unfortunately, their unsuitable management and inconsiderable disposal into the ecosystem, including surface-, ground-and seawater, has led to severe environmental and health concerns, since such non-degradable compounds retain their stability and consequently can escape conventional activated sludge wastewater treatments [4,5]. Moreover, conventional technologies that are currently employed in sewage treatment plants, for example, exhibit low efficacy in the removal of such organic pollutants found at trace levels. As a result, the latter accumulate into aquatic systems and distribute into the environment [6]. Consequently, researchers worldwide have been focusing on the development of new and effective methods that could be employed in the removal of pharmaceutical and other contaminants from wastewater [7,8]. Among others, these are based on various processes, including coagulation, filtration, adsorption, sedimentation and photodegradation [9][10][11]. Of all the above, adsorption is considered to be one of the most efficient, simple, inexpensive and eco-friendly processes [12].

Membrane Fabrication
A single-nozzle, custom-made electrospinning setup, which is schematically depicted in Figure 1, was used in the fabrication of fibrous membranes consisting of blended PMMA xco-PDEAEMA y /PVP electrospun polymer fibers. The electrospinning experiments were carried out at room temperature (25 • C) and under~35-40% humidity levels. For the production of the PMMA x -co-PDEAEMA y /PVP electrospun fibrous membranes, a homogeneous solution containing PMMA x -co-PDEAEMA y (0.75 g) and PVP (0.75 g) (i.e., a 1:1 wt. polymer mixture) was initially prepared in CHCl 3 (5 mL) at a 30% w/v polymer solution concentration upon stirring for 24 h at room temperature. Afterwards, the resulting homogeneous PMMA x -co-PDEAEMA y /PVP solution was transferred into a 10 mL glass syringe connected with a metallic needle (16 G) and the flow rate was set at 2.5 mL·h −1 using a flow controller (KDS 789252, KD Scientific Inc., Holliston, Massachusetts, USA. The applied voltage and distance between the tip of the needle and the stainless-steel grounded collector were set at 20 kV and 15 cm, respectively. The PMMA-co-PDEAEMA/PVP fibrous membrane was then thermally crosslinked upon heating at ca. 180 • C for 5 h, thus rendering it insoluble in aqueous media. The magnetic functionalization of the crosslinked PMMA-co-PDEAEMA/PVP fibrous membrane was carried out by the chemical co-precipitation of Fe(III) and Fe(II) cations in a 2:1 molar ratio under weak basic conditions. Prior to the reaction, deionized water and ammonium hydroxide solution (Sigma-Aldrich, St. Louis, MI, USA, 25% (v/v) were purged with high-purity N 2 for 30 min to remove oxygen. The reaction was performed under continuous nitrogen flow.
A typical experimental procedure is described as follows: Initially, (0.147 g, 0.54 mmol) FeCl 3 .6H 2 O was transferred into a glass vial (20 mL). Degassed, deionized water (3 mL) was subsequently added, followed by the addition of FeCl 2 .4H 2 O (0.0537 g, 0.27 mmol) dissolved in degassed, deionized water (3 mL). The resulting mixture was left to stir under an inert nitrogen atmosphere for 15 min, resulting in an orange-colored, transparent and homogeneous solution. In the meantime, the PMMA x -co-PDEAEMA y /PVP crosslinked fibrous membrane (32 mg) was fully immersed in a degassed aqueous solution (6 mL). Afterwards, the Fe(III)/Fe(II) aqueous solution was transferred with a syringe to the membrane-containing solution, followed by stirring for 15 min before the dropwise addition of the ammonium hydroxide solution (0.36 mL) while purging with extra-pure N 2 for an additional 30 min. During this time, the solution immediately turned from homogeneous orange-colored to non-homogeneous dark-brown-colored, indicating the formation of iron oxide (Fe x O y ) nanoparticles. The magnetically functionalized PMMA x -co-PDEAEMA y /PVP-Fe x O y fibrous membrane was then collected by magnetic separation and washed several times with deionized water to remove the unbound Fe x O y nanoparticles and unreacted products. The membrane was then dried at 40 • C under vacuum for 24 h. The magnetic functionalization of the crosslinked PMMA-co-PDEAEMA/PVP fibrous membrane was carried out by the chemical co-precipitation of Fe(III) and Fe(II) cations in a 2:1 molar ratio under weak basic conditions. Prior to the reaction, deionized water and ammonium hydroxide solution (Sigma-Aldrich, St. Louis, MI, USA, 25% (v/v) were purged with high-purity N 2 for 30 min to remove oxygen. The reaction was performed under continuous nitrogen flow.
A typical experimental procedure is described as follows: Initially, (0.147 g, 0.54 mmol) FeCl3.6H2O was transferred into a glass vial (20 mL). Degassed, deionized water (3 mL) was subsequently added, followed by the addition of FeCl2.4H2O (0.0537 g, 0.27 mmol) dissolved in degassed, deionized water (3 mL). The resulting mixture was left to stir under an inert nitrogen atmosphere for 15 min, resulting in an orange-colored, transparent and homogeneous solution. In the meantime, the PMMAx-co-PDEAEMAy/PVP crosslinked fibrous membrane (32 mg) was fully immersed in a degassed aqueous solution (6 mL). Afterwards, the Fe(III)/Fe(II) aqueous solution was transferred with a syringe to the membrane-containing solution, followed by stirring for 15 min before the dropwise addition of the ammonium hydroxide solution (0.36 mL) while purging with extra-pure N2 for an additional 30 min. During this time, the solution immediately turned from homogeneous orange-colored to non-homogeneous dark-brown-colored, indicating the formation of iron oxide (FexOy) nanoparticles. The magnetically functionalized PMMAx-co-PDEAEMAy/PVP-FexOy fibrous membrane was then collected by magnetic separation and washed several times with deionized water to remove the unbound FexOy nanoparticles and unreacted products. The membrane was then dried at 40 °C under vacuum for 24 h.

Membrane Characterization
Molecular characterization of the PMMAx-co-PDEAEMAy random copolymer was carried out by size exclusion chromatography (SEC) supplied by PSS Polymer Standards Service GmbH (Mainz, Germany) and nuclear magnetic resonance ( 1 H NMR) spectroscopy (Avance Brucker 500 MHz spectrometer, Bruker, Billerica, MA, USA).
SEC was used to determine the average molar mass (MM) and molar mass distribution (MMD) of the PMMAx-co-PDEAEMAy random copolymer, using equipment supplied

Membrane Characterization
Molecular characterization of the PMMA x -co-PDEAEMA y random copolymer was carried out by size exclusion chromatography (SEC) supplied by PSS Polymer Standards Service GmbH (Mainz, Germany) and nuclear magnetic resonance ( 1 H NMR) spectroscopy (Avance Brucker 500 MHz spectrometer, Bruker, Billerica, MA, USA).
SEC was used to determine the average molar mass (MM) and molar mass distribution (MMD) of the PMMA x -co-PDEAEMA y random copolymer, using equipment supplied by Polymer Standards Service (PSS). All measurements were carried out at room temperature using Styragel HR 3 and Styragel HR 4 columns. THF was used as a mobile phase (flow rate: 1 mL min −1 ). A Waters 515 isocratic pump was used for this purpose while the refractive index was measured with a Waters 2414 refractive index detector. Poly(methyl methacrylate) (PMMA) standards with a low polydispersity index (PDI; MWs of 739,000, 446,000, 270,000, 126,000, 65,000, 31,000, 14,400, 4200, 1580, 670, 450 and 102 (methyl isobutyrate) g mol −1 ), supplied by PSS, were used in system calibration.
The 1 H NMR spectrum of the PMMA x -co-PDEAEMA y random copolymer was recorded in CDCl 3 with tetramethylsilane (TMS) used as an internal standard, using an Avance Bruker 500 MHz spectrometer (Bruker, Billerica, MA, USA) equipped with an Ultrashield magnet.
The morphology of the produced membranes was analyzed by scanning electron microscopy (SEM) (Vega TS5136LS-Tescan). All samples were gold-sputtered (~100 nm) (K575X Turbo Sputter Coater-Emitech, Quorum Technologies Ltd., West-Sussex, UK) prior to SEM analysis for reduction of the effect of surface charging. Transmission electron microscopy (TEM) was utilized to visualize the iron oxide (Fe x O y ) nanoparticles that were anchored onto the fibers' surfaces. For this purpose, a TECNAI F30 G2 S-TWIN microscope operating at 300 kV and equipped with an energy-dispersive X-ray spectrometer (EDX) (FEI Company, the Netherlands) was used while samples were placed into a double copper grid (oyster).
Tensile experiments were performed using a high-precision mechanical testing system (Instron 5944, Norwood, MA, USA). Orthogonal specimens were prepared with dimensions of 5.0 × 6.0 × 1.0 mm (length × width × thickness) and were held by two grips. Stressstrain experiments were performed to measure the elastic behavior of the material. The specimens were stretched to 25% strain with a strain rate of 0.5 mm/min. The stress was calculated as the force measured on the load cell divided by the initial area of the specimen (i.e., 1st Piola-Kirchhoff stress) and the strain was calculated as the displacement ∆l divided by the initial length of the specimen. The Young's modulus was calculated from the slope of the linear part of the stress-strain curves for low strains (< 5%). Six specimens were tested (n = 6).
The X-ray diffraction pattern of the magnetically functionalized membrane was obtained using Rigaku (30 kV, 25 mA) with λ = 1.5405 Å (Cu). The magnetic properties of the produced magnetically functionalized fibrous adsorbent was measured by vibrating sample magnetometry (VSM) using an ADE Technologies VSM880 magnetometer (ADE Technologies inc., Lowell, MA, USA). The measurement was performed at room temperature, in the magnetic field intensity range of −1000 kA/m-1000 kA/m.

Adsorption Studies in Synthetic Aqueous Media
To evaluate the performance of the produced electrospun PMMA x -co-PDEAEMA y /PVP (pristine) and PMMA x -co-PDEAEMA y /PVP-Fe x O y (magnetoactive) fibrous membranes in the adsorption of OFL from synthetic aqueous solutions, batch adsorption experiments were performed under ambient conditions. All experiments were performed in glass vials (20 mL) with a plastic snap-cap.
OFL adsorption kinetic studies were performed by UV-vis spectrophotometry (Jasco V-630, Jasco Corporation, Tokyo, Japan) at room temperature. OFL was dissolved in deionized water (solution concentration: 0.1 g·L −1 ), and the resulting solution was used as stock solution (solution pH = 6.8). OFL aqueous solutions of various concentrations (0.78-18.75 mg·L −1 ) were obtained upon diluting the stock solution with deionized water.
For investigating the effect of pH on the membranes' adsorption performance, solution pH was adjusted by adding 0.01 M and/or 0.05 M aqueous HCl solution. Subsequently, a dried membrane sample (10 mg) was immersed in an OFL aqueous solution (5 mL, initial concentration of 0.01875 g·L −1 ) prepared at different pH values (pH = 4.0 and pH = 6.0). At specific time intervals, an aliquot was extracted from the solution and placed in the UV-vis spectrophotometer for recording the UV-vis spectrum of the supernatant solution containing OFL (adsorption wavelength: 287 nm). Upon the completion of each measurement, the aliquot was returned back into the vial. The OFL removal efficiency (%) was calculated using an absorbance (recorded at 287 nm) versus concentration (g·L −1 ) calibration curve (correlation coefficient, R 2 = 0.996) ( Figure 2). All the adsorption experiments were performed in triplicate for each system in order to verify the repeatability of the measurements.
For investigating the effect of the initial OFL concentration, a specific amount of the magnetoactive fibrous adsorbent (10 mg) was placed in aqueous solutions (5 mL) prepared in DI water containing various OFL concentrations (0.78, 2.34, 6.25 and 12.5 mg·L −1 ). In all cases, the pH value was adjusted to 4. The UV-vis spectrum of the supernatant solution was then recorded to evaluate the amount of the adsorbed OFL, denoted as adsorption capacity, q e (mg·g −1 ), by measuring the absorbance at 287 nm. The latter as well as the % removal efficiency (% q e ) were calculated using the following equations: [40]  where q e (mg·g −1 ) is the adsorbed amount of OFL, C 0 (mg·L −1 ) and C aq (mg·L −1 ) are the initial and equilibrium concentrations of the drug in solution, V (L) is the volume of OFL solution and W (mg) is the weight of the dry fibrous adsorbent.
Nanomaterials 2022, 12, x FOR PEER REVIEW 6 of 21 calibration curve (correlation coefficient, R 2 = 0.996) ( Figure 2). All the adsorption experiments were performed in triplicate for each system in order to verify the repeatability of the measurements. For investigating the effect of the initial OFL concentration, a specific amount of the magnetoactive fibrous adsorbent (10 mg) was placed in aqueous solutions (5 mL) prepared in DI water containing various OFL concentrations (0.78, 2.34, 6.25 and 12.5 mg L −1 ). In all cases, the pH value was adjusted to 4. The UV-vis spectrum of the supernatant solution was then recorded to evaluate the amount of the adsorbed OFL, denoted as adsorption capacity, qe (mg•g −1 ), by measuring the absorbance at 287 nm. The latter as well as the % removal efficiency (% qe) were calculated using the following Equations: [40] where qe (mg.g −1 ) is the adsorbed amount of OFL, Co (mg.L −1 ) and Caq (mg. L −1 ) are the initial and equilibrium concentrations of the drug in solution, V (L) is the volume of OFL solution and W (mg) is the weight of the dry fibrous adsorbent.

Ofloxacin Removal from Urban Wastewater
A solution containing OFL (concentration: 2.34 mg L −1 ) was prepared by spiking the appropriate mass of the compound into the secondary treated effluent sample collected from the Urban Wastewater (UWW) treatment plant located on the premises of the University of Cyprus. The incorporation of a higher quantity of the OFL antibiotic into the UWW sample than that typically found in real wastewater samples was preferred in order to enable the detection and measurement of residual OFL by employing typical analytical methods. Solution pH was adjusted at ~ 4 by using an HCl aqueous solution (0.05-0.01 M). A specific amount of the dried magnetoactive fibrous membrane (10 mg) was immersed into the solution and aliquots were withdrawn at various time intervals and

Ofloxacin Removal from Urban Wastewater
A solution containing OFL (concentration: 2.34 mg·L −1 ) was prepared by spiking the appropriate mass of the compound into the secondary treated effluent sample collected from the Urban Wastewater (UWW) treatment plant located on the premises of the University of Cyprus. The incorporation of a higher quantity of the OFL antibiotic into the UWW sample than that typically found in real wastewater samples was preferred in order to enable the detection and measurement of residual OFL by employing typical analytical methods. Solution pH was adjusted at~4 by using an HCl aqueous solution (0.05-0.01 M). A specific amount of the dried magnetoactive fibrous membrane (10 mg) was immersed into the solution and aliquots were withdrawn at various time intervals and further analyzed by UV-vis spectrophotometry to record the characteristic absorbance signal of OFL appearing at 287 nm.

Desorption Studies
Desorption studies were performed at various temperatures (25, 37 and 60 • C) by immersing the OFL-containing magnetoactive fibrous adsorbent (10 mg) in alkali solution (5 mL) (NaOH 0.005 M-0.01 M) at pH 8.5, followed by the removal of the supernatant solution at specific time intervals. UV-vis spectrophotometry was used to record the optical density of the collected solution at 287 n so as to determine the concentration of the desorbed OFL. The desorption (%) of OFL was determined based on Equation (3):

Polymer Synthesis and Molecular Characterization
A PMMA x -co-PDEAEMA y random copolymer comprising both hydrophilic/cationic (DEAEMA) and hydrophobic (MMA) units was successfully synthesized by conventional free radical polymerization.
The polymerization methodology followed, as well as the chemical structures of the monomers (MMA, DEAEMA) and the initiator (AIBN), are provided in Figure 3. SEC was employed to determine the number average molar mass (M n = 69,104 g·mol −1 ) and molar mass distribution (MMD = 2.60) of the PMMA x -co-PDEAEMA y copolymer. As expected, the polymer possessed a relatively high PDI due to the non-controlled character of the free radical polymerization process [41]. Moreover, the copolymer chemical composition was evaluated using 1 H NMR spectroscopy. More specifically, the molar ratio of the two repeating units (MMA, DEAEMA) incorporated within the copolymer chain was determined to be 1:0.7, respectively, by assigning the characteristic resonance peaks appearing in the 1 H NMR spectrum, corresponding to each one of the two monomer units.

Polymer Synthesis and Molecular Characterization
A PMMAx-co-PDEAEMAy random copolymer comprising both hydrophilic/cationic (DEAEMA) and hydrophobic (MMA) units was successfully synthesized by conventional free radical polymerization.
The polymerization methodology followed, as well as the chemical structures of the monomers (MMA, DEAEMA) and the initiator (AIBN), are provided in Figure 3. SEC was employed to determine the number average molar mass (Mn = 69,104 g·mol −1 ) and molar mass distribution (MMD = 2.60) of the PMMAx-co-PDEAEMAy copolymer. As expected, the polymer possessed a relatively high PDI due to the non-controlled character of the free radical polymerization process [41]. Moreover, the copolymer chemical composition was evaluated using 1 H NMR spectroscopy. More specifically, the molar ratio of the two repeating units (MMA, DEAEMA) incorporated within the copolymer chain was determined to be 1:0.7, respectively, by assigning the characteristic resonance peaks appearing in the 1 H NMR spectrum, corresponding to each one of the two monomer units.

Membrane Fabrication and Morphological Characterization
Electrospinning was employed to fabricate blended fibrous membranes consisting of PMMAx-co-PDEAEMAy and PVP, as schematically shown in Figure 4. Through employment of the optimum electrospinning conditions (polymer solution concentration: 30 w/v; Figure 3. Synthetic procedure followed for the preparation of the PMMA x -co-PDEAEMA y random copolymer by free radical polymerization and chemical structures of the MMA and DEAEMA monomeric units.

Membrane Fabrication and Morphological Characterization
Electrospinning was employed to fabricate blended fibrous membranes consisting of PMMA x -co-PDEAEMA y and PVP, as schematically shown in Figure 4. Through employment of the optimum electrospinning conditions (polymer solution concentration: 30 w/v; applied voltage: 20 kV; needle gauge: 16 G; needle-to-collector distance: 15 cm; flow rate: 2.5 mL·h −1 ), the PMMA x -co-PDEAEMA y /PVP electrospun fibrous membrane was successfully produced. In order to render the as-prepared membrane insoluble in water, a thermal treatment process was employed. Based on a previous study by our group, [42] an FTIR analysis performed on thermally crosslinked PVP-containing electrospun fibrous membranes revealed the existence of small changes appearing around 1250 cm −1 , which was assigned to C-N stretching and the bands at 880 cm −1 , corresponding to the breathing vibration of the pyrrolidone ring and thus indicating the success of the PVP crosslinking process. The latter was further supported by the fact that the thermally crosslinked membranes were insoluble in aqueous media, in contrast to the non-crosslinked analogues.
The post-magnetization process employed in the preparation of the magnetoactive PMMA x -co-PDEAEMA y /PVP-Fe x O y electrospun fibrous membrane involved the chemical co-precipitation of Fe(II) and Fe(III) under alkaline conditions [43]. By performing the above-mentioned chemical reaction in the presence of the PMMA x -co-PDEAEMA y /PVP fibrous membrane, the magnetic iron oxide nanoparticles produced were anchored onto the fibers' surfaces, as schematically presented in Figure 5a. Moreover, as seen in Figure 5b, the resulting brown-colored magnetically functionalized membrane could be attracted by a permanent magnet, thus providing the possibility of its removal from aquatic systems by applying an external magnetic field. According to Huang and co-workers, tertiary amino-functionalities are capable of binding onto the surfaces of iron oxide NPs, which justifies the effective anchoring of the in situ synthesized Fe x O y NPs onto the surfaces of the DEAEMA-containing fibers [44]. mal treatment process was employed. Based on a previous study by our group, [42] an FTIR analysis performed on thermally crosslinked PVP-containing electrospun fibrous membranes revealed the existence of small changes appearing around 1250 cm −1 , which was assigned to C-N stretching and the bands at 880 cm −1 , corresponding to the breathing vibration of the pyrrolidone ring and thus indicating the success of the PVP crosslinking process. The latter was further supported by the fact that the thermally crosslinked membranes were insoluble in aqueous media, in contrast to the non-crosslinked analogues. The post-magnetization process employed in the preparation of the magnetoactive PMMAx-co-PDEAEMAy/PVP-FexOy electrospun fibrous membrane involved the chemical co-precipitation of Fe(II) and Fe(III) under alkaline conditions [43]. By performing the above-mentioned chemical reaction in the presence of the PMMAx-co-PDEAEMAy/PVP fibrous membrane, the magnetic iron oxide nanoparticles produced were anchored onto the fibers' surfaces, as schematically presented in Figure 5a. Moreover, as seen in Figure  5b, the resulting brown-colored magnetically functionalized membrane could be attracted by a permanent magnet, thus providing the possibility of its removal from aquatic systems by applying an external magnetic field. According to Huang and co-workers, tertiary amino-functionalities are capable of binding onto the surfaces of iron oxide NPs, which justifies the effective anchoring of the in situ synthesized FexOy NPs onto the surfaces of the DEAEMA-containing fibers [44]. SEM was employed to obtain information on the morphology of the produced materials. Figure 6 provides characteristic SEM images of the as-prepared, non-crosslinked PMMAx-co-PDEAEMAy/PVP fibers (Figure 6a), the corresponding crosslinked fibers (Figure 6b) and the surface-functionalized PMMAx-co-PDEAEMAy/PVP/FexOy-crosslinked fibers ( Figure 6c). As seen, the produced fibrous membranes obtained under the optimum electrospinning conditions consisted of continuous, bead-free and cylindrical fibers with smooth surfaces. Their average diameters were determined to be 1.055 ± 0.440 μm. However, upon thermal crosslinking, partial merging of the fibers was observed, accompanied by an increase in their average diameters and decrease in homogeneity (7.005 ± 5.975 μm). This phenomenon might be attributed to the one-step thermal treatment process employed at 180 °C . This effect can be diminished by following a milder thermal treatment protocol involving a three-step heating process [42]. SEM was employed to obtain information on the morphology of the produced materials. Figure 6 provides characteristic SEM images of the as-prepared, non-crosslinked PMMA x -co-PDEAEMA y /PVP fibers (Figure 6a), the corresponding crosslinked fibers (Figure 6b) and the surface-functionalized PMMA x -co-PDEAEMA y /PVP/Fe x O y -crosslinked fibers ( Figure 6c). As seen, the produced fibrous membranes obtained under the optimum electrospinning conditions consisted of continuous, bead-free and cylindrical fibers with smooth surfaces. Their average diameters were determined to be 1.055 ± 0.440 µm. However, upon thermal crosslinking, partial merging of the fibers was observed, accompanied by an increase in their average diameters and decrease in homogeneity (7.005 ± 5.975 µm).
This phenomenon might be attributed to the one-step thermal treatment process employed at 180 • C. This effect can be diminished by following a milder thermal treatment protocol involving a three-step heating process [42]. TEM was also employed to study the morphology of the magnetically functionalized fibrous adsorbents.
As seen in the TEM bright-field images provided in Figure 7a, FexOy NP aggregates could be observed on the fibers' surfaces, indicating their successful anchoring onto the fibers during the post-magnetization step, in agreement with our previous study involving the post-magnetization of chitosan-based electrospun nanofibers [43]. Furthermore, the EDX spectrum provided in Figure 7b shows the presence of Fe, O, N and C as the major elements in the sample (element Cu comes from the copper grid). In the case of the post-magnetized fibrous membrane, the presence of Fe x O y NP aggregates on the membrane's surface could be clearly observed. Furthermore, although the morphology and average diameters of the fibers remained relatively unaffected, upon fiber hydration during the chemical co-precipitation process, some morphological changes could be observed, i.e., partial fiber swelling, in line with previous studies [45,46].
TEM was also employed to study the morphology of the magnetically functionalized fibrous adsorbents.
As seen in the TEM bright-field images provided in Figure 7a, Fe x O y NP aggregates could be observed on the fibers' surfaces, indicating their successful anchoring onto the fibers during the post-magnetization step, in agreement with our previous study involving the post-magnetization of chitosan-based electrospun nanofibers [43]. Furthermore, the EDX spectrum provided in Figure 7b shows the presence of Fe, O, N and C as the major elements in the sample (element Cu comes from the copper grid).

Determination of the FexOy Nanocrystalline Phase
XRD was employed to determine the nanocrystalline phase adopted by the FexOy NPs that were generated in situ and simultaneously deposited onto the surfaces of the fibrous adsorbent. Figure 8 provides the XRD diffraction pattern of the magnetically functionalized electrospun nanocomposite membrane. Six broad peaks appear at 2θ∼30°, 36°, 43°, 54°, 58° and 63°, indicating the presence of Fe3O4 NPs, in agreement with previously reported studies [47][48][49][50].

Determination of the Fe x O y Nanocrystalline Phase
XRD was employed to determine the nanocrystalline phase adopted by the Fe x O y NPs that were generated in situ and simultaneously deposited onto the surfaces of the fibrous adsorbent. Figure 8 Figure 9 illustrates the magnetic hysteresis (M vs. H) curve obtained at 300 K. The sample is superparamagnetic with negligible remanence and coercivity (Mr = 0.035 emu/g, Hc = 0.08 kA/m). The saturation magnetization of the sample, measured at 1000 kA/m, was 12.3 emu/g, which indicated a high degree of magnetic loading. Using the magnetite saturation magnetization 93 emu/g, the magnetic loading of the sample was calculated to be 13.2 wt.%. From the data fit (R 2 = 0.99998) with a magnetization theoretical model, [51] the iron oxide nanoparticles' magnetic diameter was found to be 5.4 ± 2.3 nm. The fit curve is presented in Figure 8. According to Tolmacheva et al., [52] hypercrosslinked polystyrene (HCPS) and Fe3O4 nanoparticles (HCPS-Fe3O4) adsorbents were tested for the removal of various tetracycline antibiotics separation from aqueous media. In those systems, Ms was found to range between 1 and 10 emu g −1 , which was found to be in good agreement with the value recorded in the present study.  Figure 9 illustrates the magnetic hysteresis (M vs. H) curve obtained at 300 K. The sample is superparamagnetic with negligible remanence and coercivity (M r = 0.035 emu/g, H c = 0.08 kA/m). The saturation magnetization of the sample, measured at 1000 kA/m, was 12.3 emu/g, which indicated a high degree of magnetic loading. Using the magnetite saturation magnetization 93 emu/g, the magnetic loading of the sample was calculated to be 13.2 wt.%. From the data fit (R 2 = 0.99998) with a magnetization theoretical model, [51] the iron oxide nanoparticles' magnetic diameter was found to be 5.4 ± 2.3 nm. The fit curve is presented in Figure 8. According to Tolmacheva et al., [52] hypercrosslinked polystyrene (HCPS) and Fe 3 O 4 nanoparticles (HCPS-Fe 3 O 4 ) adsorbents were tested for the removal of various tetracycline antibiotics separation from aqueous media. In those systems, Ms was found to range between 1 and 10 emu·g −1 , which was found to be in good agreement with the value recorded in the present study.

Mechanical Properties
The mechanical behavior of the pristine and magnetically functionalized crosslinked electrospun fibrous membranes was investigated under tensile loading conditions. Representative stress-strain curves for each case are provided in Figure 10. The Young's modulus for each membrane was calculated from the slope of the linear part of the stress-strain curves for low strains (<5%). The corresponding average values were 2.09 ± 1.09 MPa for pristine and 4.56 ± 1.86 MPa for magnetoactive membranes; the difference is statistically significant. Furthermore, a yield point was observed at~7% strain for both membrane types whereas the magnetically functionalized membranes exhibited a higher yield stress, i.e., 0.12 ± 0.03 MPa for pristine and 0.23 ± 0.05 MPa for magnetoactive membranes, with statistically significant difference. The mechanical enhancement observed in the case of the magnetically functionalized membrane might be attributed to the presence of the Fe x O y NPs onto the fibers' surfaces, which may have acted as crosslinking points among the PVP chains (through the development of coordination bonds with the C=O group) and the DEAEMA moieties (through the tertiary amino functionalities) existing on the fibers' surfaces [53,54].

Mechanical Properties
The mechanical behavior of the pristine and magnetically functionalized crosslinked electrospun fibrous membranes was investigated under tensile loading conditions. Representative stress-strain curves for each case are provided in Figure 10. The Young's modulus for each membrane was calculated from the slope of the linear part of the stressstrain curves for low strains (<5%). The corresponding average values were 2.09 ± 1.09 MPa for pristine and 4.56 ± 1.86 MPa for magnetoactive membranes; the difference is statistically significant. Furthermore, a yield point was observed at ~7% strain for both membrane types whereas the magnetically functionalized membranes exhibited a higher yield stress, i.e., 0.12 ± 0.03 MPa for pristine and 0.23 ± 0.05 MPa for magnetoactive membranes, with statistically significant difference. The mechanical enhancement observed in the case of the magnetically functionalized membrane might be attributed to the presence of the FexOy NPs onto the fibers' surfaces, which may have acted as crosslinking points among the PVP chains (through the development of coordination bonds with the C=O group) and the DEAEMA moieties (through the tertiary amino functionalities) existing on the fibers' surfaces [53,54].

Ofloxacin Removal from Synthetic Aqueous Media
The investigation of OFL removal from synthetic aqueous media in the presence o either the pristine or the magnetically functionalized electrospun fibrous adsorbent wa carried out by conducting batch-type experiments. UV-vis spectrophotometry was used to monitor the adsorption process.
pH is one of the most important parameters that govern adsorption efficiency [55][56][57][58]. In the present study, solution pH may have strongly affected the membranes' remova efficiency, since OFL possesses several functional groups that are influenced by pH. More precisely, OFL has two ionizable functional groups and thus exhibits two different pK

Ofloxacin Removal from Synthetic Aqueous Media
The investigation of OFL removal from synthetic aqueous media in the presence of either the pristine or the magnetically functionalized electrospun fibrous adsorbent was carried out by conducting batch-type experiments. UV-vis spectrophotometry was used to monitor the adsorption process. pH is one of the most important parameters that govern adsorption efficiency [55][56][57][58]. In the present study, solution pH may have strongly affected the membranes' removal efficiency, since OFL possesses several functional groups that are influenced by pH. More precisely, OFL has two ionizable functional groups and thus exhibits two different pK a values, as depicted in Figure 11. The 3-carboxyl group presented a pk a equal to 6.10 and the nitrogen atom of piperazinyl group a pk a equal to 8.28. Consequently, at pH < 6.1, the cationic form dominates whereas at pH > 8.28, the anionic form exists. Within the pH range of 6.1-8.28, OFL exists partially in both the zwitterionic (OFL ± ) and the neutral (OFL 0 ) form [59].

Ofloxacin Removal from Synthetic Aqueous Media
The investigation of OFL removal from synthetic aqueous media in the presence of either the pristine or the magnetically functionalized electrospun fibrous adsorbent was carried out by conducting batch-type experiments. UV-vis spectrophotometry was used to monitor the adsorption process.
pH is one of the most important parameters that govern adsorption efficiency [55][56][57][58]. In the present study, solution pH may have strongly affected the membranes' removal efficiency, since OFL possesses several functional groups that are influenced by pH. More precisely, OFL has two ionizable functional groups and thus exhibits two different pKa values, as depicted in Figure 11. The 3-carboxyl group presented a pka equal to 6.10 and the nitrogen atom of piperazinyl group a pka equal to 8.28. Consequently, at pH < 6.1, the cationic form dominates whereas at pH > 8.28, the anionic form exists. Within the pH range of 6.1-8.28, OFL exists partially in both the zwitterionic (OFL ± ) and the neutral (OFL 0 ) form [59]. Adsorption kinetic measurements were carried out by immersing the fibrous membranes in OFL-containing aqueous solutions for 24 h and recording the OFL absorbanceat 287 nm at different time intervals after membrane incubation. In order to investigate the Adsorption kinetic measurements were carried out by immersing the fibrous membranes in OFL-containing aqueous solutions for 24 h and recording the OFL absorbanceat 287 nm at different time intervals after membrane incubation. In order to investigate the adsorption dependency on pH, two different experiments were carried out at pH = 4 and pH = 6. The adsorption kinetic plots corresponding to the two membrane types recorded under the above-mentioned pH conditions are provided in Figure 12. According to the experimental data provided in Figure 12, the magnetic fibrous adsorbent presented a significantly higher (two-to threefold) adsorption efficiency compared to the non-magnetic analogue at both pH values. More precisely, a 13% and 9% OFL According to the experimental data provided in Figure 12, the magnetic fibrous adsorbent presented a significantly higher (two-to threefold) adsorption efficiency compared to the non-magnetic analogue at both pH values. More precisely, a 13% and 9% OFL removal was observed in the case of the pristine fibrous membrane at pH 4 and 6, respectively, whereas the percentage removal was increased to 39% and 21% when the magnetically functionalized fibrous adsorbent was used instead. The same positive effect of magnetic nanoparticle functionalization on the adsorption efficacy of previously reported electrospun fibrous membranes employed as substrates for the removal of various contaminants, including antibiotics from aqueous media, was also observed [28,29,60]. Exemplarily, Liu et al. demonstrated the tetracycline removal from aqueous media at a pH range of 4-6, employing a Fe 3 O 4 -functionalized polyacrylonitrile electrospun membrane [60].
The differences observed in the adsorption efficiency of the non-magnetic fibrous membrane at pH 4 and 6 could be attributed to the fact that at pH 4, both the tertiary amino-functionalities in DEAEMA and the OFL amino group were found in the cationic form, which could have resulted in the development of electrostatic repulsive forces that eventually led to lower adsorption efficiency. However, at lower pH, hydrogen bonding interactions existed between the carbonyl group of PVP and the hydrogen of the OFL carboxyl group, which was found in its neutral (non-ionized) form. Consequently, the DEAEMA-OFL repulsive forces could not prevail over the PVP-OFL H-bond interactions, probably due to the smaller percentage of the DEAEMA moieties within the fibrous membrane.
The improved adsorption performance observed in the case of the Fe x O y -containing electrospun fibrous membrane, especially at the lowest pH = 4, was attributed to the presence of the Fe x O y NPs on the fibers' surfaces, as revealed by SEM and TEM, that provide additional binding sites for OFL adsorption. Specifically, coordination complexes may form between the carbonyl group of OFL and the hydrous oxide (Fe-OH 2 + ) that is generated at low pH values [61][62][63].
This result is in line with our group's previous studies dealing with magnetically functionalized electrospun fibrous membranes that were evaluated as adsorbents for the removal of U(VI) and Eu(III) from aqueous solutions, [28,29] thus highlighting the significance of magnetic functionalization in the development of effective adsorbents destined for use in water-remediation processes.
The maximum adsorption capacity (q max ) of the magnetically functionalized fibrous membrane was determined by immersing the adsorbent in aqueous solutions of various OFL concentrations at room temperature and at pH 4 and recording the UV-vis spectrum of the supernatant solution after 24 h. Through use of the absorbance (at 287 nm) vs. OFL concentration calibration curve (provided in Figure 2), the equilibrium concentration of OFL in the solution and the equilibrium adsorbed amount of OFL per unit mass of adsorbent, denoted as C e (mg·L −1 ) and q e (mg·g −1 ), respectively, could be determined. By fitting the experimental data to the Langmuir adsorption model expressed mathematically by Equation (4) and plotting 1/q e versus 1/C e (Figure 13), the maximum adsorption capacity q max (mg·g −1 ) and the Langmuir adsorption equilibrium constant K d (L·mg −1 ) that reflects the adsorption affinity of the binding sites were determined to be 20.5 (mg·g −1 ) and 0.068 (L·mg −1 ), respectively.

Removal of OFL from Urban Wastewater
The PMMA x -co-PDEAEMA y /PVP-Fe x O y crosslinked fibrous membrane exhibiting the highest adsorption efficiency in the removal of OFL from synthetic aqueous solutions at pH = 4 was selected to be further evaluated as a substrate for the removal of OFL from secondary urban wastewater (UWW). The latter was spiked with OFL at a concentration of 2.34 mg·L −1 . By recording the characteristic absorbancesignal of free (unbound) OFL appearing at 287 nm at different time intervals after membrane incubation, the % remaining of OFL vs. time plot could be constructed (Figure 14), demonstrating a high removal efficiency by reaching 80% after 24 h incubation time. Equation 4 and plotting 1/qe versus 1/Ce (Figure 13), the maximum adsorption capacity qmax (mg g −1 ) and the Langmuir adsorption equilibrium constant Kd (L·mg −1 ) that reflects the adsorption affinity of the binding sites were determined to be 20.5 (mg·g −1 ) and 0.068 (L·mg −1 ), respectively.

Removal of OFL from Urban Wastewater
The PMMAx-co-PDEAEMAy/PVP-FexOy crosslinked fibrous membrane exhibiting the highest adsorption efficiency in the removal of OFL from synthetic aqueous solutions at pH = 4 was selected to be further evaluated as a substrate for the removal of OFL from secondary urban wastewater (UWW). The latter was spiked with OFL at a concentration of 2.34 mg L −1 . By recording the characteristic absorbancesignal of free (unbound) OFL

Desorption Studies
Desorption of the adsorbed OFL was realized by immersing the OFL-containing magnetoactive fibrous membrane in alkali solutions at various temperatures. The desorption profile of OFL recorded at 25 °C , 37 °C and 60 °C is provided in Figure 15. As seen, a rapid OFL release was observed within the first 10 min in all cases while temperature was found to play a significant role in the desorption process, since desorption % of 46, 82 and

Desorption Studies
Desorption of the adsorbed OFL was realized by immersing the OFL-containing magnetoactive fibrous membrane in alkali solutions at various temperatures. The desorption profile of OFL recorded at 25 • C, 37 • C and 60 • C is provided in Figure 15. As seen, a rapid OFL release was observed within the first 10 min in all cases while temperature was found to play a significant role in the desorption process, since desorption % of 46, 82 and 100 were recorded at 25 • C, 37 • C and 60 • C, respectively. This result agrees with a previous study by Mohhamad et al. in which the authors stated that an increase in temperature may raise the kinetic energy of the molecules, resulting in the gaining of higher energy than that required for the adsorption process to occur [64].

Conclusions
Magnetically functionalized electrospun microfibrous membranes containing tiary amino functionalities were fabricated and evaluated as adsorbents for the remo of the antibiotic Ofloxacin from synthetic aqueous media and urban wastewater. To ther increase the adsorption efficiency and at the same time impart magnetic proper to the produced fibrous membranes, the latter underwent a post-magnetization step sulting in the anchoring of magnetic iron oxide NPs onto the fibers' surfaces. SEM TEM verified the anchoring of FexOy NPs onto the fibers' surfaces while VSM was use study the magnetic properties of the produced fibrous nanocomposites. The latter w found to be superparamagnetic, exhibiting a high Ms value (12.3 emu/g) that co sponded to ~13% wt. magnetic loading. Moreover, through employment of a magnet tion theoretical model, a diameter of 5.4 ± 2.3 nm was calculated for the produced m netic NPs that were deposited onto the fibrous membranes during the post-magnetiza step. Furthermore, magnetic functionalization resulted in the mechanical enhancem under tensile loading conditions, since the magnetically functionalized adsorbents ex ited higher Young's modulus and higher yield stress in comparison to the pristine p mer analogues.
The adsorption capacity of the magnetically functionalized nanocomposite fibr adsorbents was evaluated as a function of various physicochemical parameters, includ the initial OFL solution concentration, the solution pH and the magnetic loading. M precisely, adsorption kinetic measurements were carried out at pH = 4 and pH = 6. In b

Conclusions
Magnetically functionalized electrospun microfibrous membranes containing tertiary amino functionalities were fabricated and evaluated as adsorbents for the removal of the antibiotic Ofloxacin from synthetic aqueous media and urban wastewater. To further increase the adsorption efficiency and at the same time impart magnetic properties to the produced fibrous membranes, the latter underwent a post-magnetization step resulting in the anchoring of magnetic iron oxide NPs onto the fibers' surfaces. SEM and TEM verified the anchoring of Fe x O y NPs onto the fibers' surfaces while VSM was used to study the magnetic properties of the produced fibrous nanocomposites. The latter were found to be superparamagnetic, exhibiting a high Ms value (12.3 emu/g) that corresponded to~13% wt. magnetic loading. Moreover, through employment of a magnetization theoretical model, a diameter of 5.4 ± 2.3 nm was calculated for the produced magnetic NPs that were deposited onto the fibrous membranes during the post-magnetization step. Furthermore, magnetic functionalization resulted in the mechanical enhancement under tensile loading conditions, since the magnetically functionalized adsorbents exhibited higher Young's modulus and higher yield stress in comparison to the pristine polymer analogues.
The adsorption capacity of the magnetically functionalized nanocomposite fibrous adsorbents was evaluated as a function of various physicochemical parameters, including the initial OFL solution concentration, the solution pH and the magnetic loading. More precisely, adsorption kinetic measurements were carried out at pH = 4 and pH = 6. In both cases, the magnetic fibrous adsorbent presented a significantly higher (two-to threefold) adsorption efficiency compared to the non-magnetic analogue while the adsorption was more effective at pH 4. Hence, to determine the maximum adsorption capacity (q max ) of the magnetically functionalized fibrous membrane, the latter was immersed in aqueous solutions of various OFL concentrations at pH 4. By fitting of the experimental data to the Langmuir adsorption model, the maximum adsorption capacity q max (mg·g −1 ) and the Langmuir adsorption equilibrium constant K d (L·mg −1 ) were determined to be 20.5 (mg·g −1 ) and 0.068 (L·mg −1 ), respectively.
The magnetically functionalized nanocomposite fibrous membrane exhibiting the highest adsorption efficiency in the removal of OFL from synthetic aqueous solutions at pH = 4 was successfully evaluated as a substrate for the removal of OFL from secondary urban wastewater, reaching 80% removal efficiency after 24 h. Finally, it was demonstrated that OFL desorption could be realized upon exposing the OFL-loaded at elevated temperatures.