Influences of Plasma Plume Length on Structural, Optical and Dye Degradation Properties of Citrate-Stabilized Silver Nanoparticles Synthesized by Plasma-Assisted Reduction

Citrate-capped silver nanoparticles (Ag@Cit NPs) were synthesized by a simple plasma-assisted reduction method. Homogenous colloidal Ag@Cit NPs solutions were produced by treating a AgNO3-trisodium citrate-deionized water with an atmospheric-pressure argon plasma jet. The plasma-synthesized Ag@Cit NPs exhibited quasi-spherical shape with an average particle diameter of about 5.9−7.5 nm, and their absorption spectra showed surface plasmon resonance peaks at approximately 406 nm. The amount of Ag@Cit NPs increased in a plasma exposure duration-dependent manner. Plasma synthesis of Ag@Cit NPs was more effective in the 8.5 cm plume jet than in the shorter and longer plume jets. A larger amount of Ag@Cit NPs were produced from the 8.5 cm plume jet with a higher pH and a larger number of aqua electrons, indicating that the synergetic effect between plasma electrons and citrate plays an important role in the plasma synthesis of Ag@Cit NPs. Plasma-assisted citrate reduction facilitates the synthesis of Ag@Cit NPs, and citrate-capped nanoparticles are stabilized in an aqueous solution due to their repulsive force. Next, we demonstrated that plasma-synthesized Ag@Cit NPs exhibited a significant degradation of methylene blue dye.

Recently, atmospheric-pressure plasma jets have received a lot of interest as a promising green means for the synthesis of nanomaterials [5,20]. Metallic nanoparticles can be produced by applying atmospheric-pressure plasma jets [5,20], dielectric barrier discharge plasma [21], gas-liquid interfacial plasmas [22], and dual plasma electrolysis [23] to aqueous metal salt solutions. Among these plasma synthesis methods, metal nanoparticle

Characteristics of the Plasma Device
In this research, an alternating current (AC)-driven argon plasma jet (Figure 1) was used for synthesis of Ag@Cit NPs. The plasma device consists of a powered needle electrode and a grounded copper tape electrode. A stainless-steel needle electrode (with a length of 50 mm, an inner diameter of 0.6 mm, and an outer diameter of 0.9 mm) was used as the power electrode. For grounding, a copper tape was fixed to the bottom of a glass beaker that contained AgNO 3 -TSC-DW. A plasma jet was created from a needle electrode inserted inside a quartz tube (with an inner diameter of 2 mm and an outer diameter of 4 mm). Argon gas entered the quartz tube through a needle, and was supplied to the needle with a gas flow rate of 500 standard cubic centimeters per minute (sccm). As plasma can propagate a long distance along an argon gas stream in a quartz tube, plasma plume length is longer for a longer quartz tube [29]. Three different plasma jets with plume lengths of 2.5, 8.5, and 12.5 cm were used to study the effect of plasma plume length on the synthesis of Ag@Cit NPs. To make plasma jets with plume lengths of 2.5, 8.5, and 12.5 cm, a powered needle electrode was inserted inside each of 6.5, 11.5, and 15.5 cm long quartz tubes ( Figure 1b). In this research, plasma jets with plume lengths of 2.5, 8.5, and 12.5 cm are called J1, J2, and J3 jets, respectively, (Figure 1c). When the plasma jet exited the nozzle of the quartz tube, the plasma plume region was at a lower pressure than the surrounding ambient air. Thus, the ambient air diffused into and directly mixed with the plasma plume below the nozzle of the quartz tube. Due to the quenching effect of atmospheric air, the plasma plume was weakened after traveling a few centimeters away from the nozzle of the quartz tube. The plasma plume lengths of J1, J2, and J3 jets were longer than their needle-to-nozzle distances. After the plasma jet exited the nozzle of the quartz tube, it propagated a few centimeters away from the nozzle. In Figure 1c, the purple color at the end of the plasma plume was due to the nitrogen emission obtained from the diffused air. After nitrogen emission, the plasma plume dissipated. In this research, the plasma jet entered the aqueous silver salt solution, and the penetration depth of the plasma plume from the water surface was adjusted to be approximately 5 mm. As the plasma plume was charged and the end of it was in contact with the water-air interface, the electric field was applied to aqua electrons and silver ions in the solutions. To investigate the reactive oxygen and nitrogen species produced by the plasma jet, optical emission spectra were recorded by a fiber optic spectrometer (HR4000, Ocean Optics, Orlando, FL, USA). Optical emission of the propagating plasma jet was collected near the water-air interface, as shown in Figure 1a. Meanwhile, Balmer emission of excited hydrogen atoms was measured in the end-on geometry to detect hydrogen produced in the AgNO 3 -TSC-DW by the plasma-liquid interactions. For this purpose, a copper tape with a circular hole was fixed to the bottom of a beaker that contained AgNO 3 -TSC-DW, and hydrogen emission was measured from the plasma light that transmitted through a hole of a copper electrode below the plasma-treated AgNO 3 -TSC-DW. To examine surface plasmon resonance effects, absorption spectra of plasma-synthesized Ag@Cit NPs were measured by an optical absorption spectrometer (Maya2000Pro, Ocean Insight, Orlando, FL, USA). The pH and electrical conductivity of the plasma-treated-AgNO 3 -TSC-DW were measured by pH and conductivity meters (PCTSTestr TM 50, Oakton, Melbourne, Australia).

Electrical Characteristics of Plasma Discharge
To measure the applied voltage and discharge current, a high-voltage probe (P6015A, Tektronix, Beaverton, OR, USA) and a current probe (CP030, LeCroy, Chestnut Ridge, NY, USA) were connected to a digital oscilloscope (WaveSurfer 434, Lecroy, New York, NY, USA). Figure 2a shows typical current and voltage waveforms of the AC-driven plasma discharge for the J2 jet. Several positive current peaks appeared with increasing applied voltage and resulted in accumulation of charges inside the quartz tube. The accumulation of positive surface charges in the positive half-cycle of the applied voltage caused discharge current peaks, and the polarity of these charges was reversed in the negative half-cycle of the applied voltage. The measured discharge currents of J1, J2, and J3 jets were 46, 43, and 34 mA, respectively, at a fixed peak voltage of 4.8 kV (rms). The current and voltage waveforms had a period of 31.5 µs, which corresponded to a frequency of 32 kHz. To determine the accumulated charge, we connected a 10 nF ceramic capacitor in series to the ground electrode and measured the voltage across it. The corresponding charge (Q)-voltage (V) Lissajous plot is shown in Figure 2b. The Q-V plot forms a closed loop, and its area represents the total energy dissipated in one cycle [30]. The dissipated powers of J1, J2, and J3 jets were measured to be 8.9, 8.5, and 4.0 W, respectively, corresponding to unit cycle energy dissipation of 0.28, 0.27, and 0.13 mJ. Electron temperature and density are key plasma parameters affecting the synthesis of metal nanoparticles [25,31,32]. Energy transfer from the thermally excited electrons to the ions increased due to the high electron densities in the discharged plasma, and the electron cooling rate was high. Higher electron densities typically imply lower electron temperatures, and vice versa. Long plasma jets feature more frequent elastic collisions between electrons and heavier particles than do shorter plasma jets. Consecutive energy impact collisions with heavy particles cooled the electron temperature of the long jet rather than that of the short jet. Electron temperature and density also have an impact on the interaction between the plasma jet and the liquid medium [26,31]. In the plasma-assisted reduction of silver ions, the amount of plasma-synthesized silver nanoparticles depends on the electron density and acidity of plasma-activated medium. Electron temperatures and densities of the J1, J2, and J3 jets at the nozzle of the quartz tube are shown in Figure 3a,b. Electron temperatures of the J1, J2, and J3 jets were 1.38, 1.31, and 1.28 eV, respectively. Electron densities of J1, J2, and J3 jets were 0.45 × 10 21 , 2.33 × 10 21 , and 4.30 × 10 21 electrons/m 3 , respectively. The collisional radiative model [33] and the convective wave packet model [34] were used to compute electron temperature and density, respectively.   [5,16]. The plasma treatment durations were 2, 5, 7.5, and 10 min. To find the optimum conditions for plasma synthesis and to study plasma synthesis routes, we investigated the effects of plasma treatment duration and plasma plume length on the physical properties of plasma-synthesized Ag@Cit NPs.

Structural and Optical Characterizations of Plasma-Synthesized Silver Nanoparticles
To investigate the structural properties of plasma-synthesized Ag@Cit NPs, highresolution transmission electron microscopy (HR-TEM) and EDS mapping were conducted using a Cs-corrected scanning transmission electron microscope (Neo ARM, JEOL, Tokyo, Japan). For measuring TEM images, the Ag@Cit NPs solution was drop-cast on a carbonstabilized Formvar-coated 200 mesh copper TEM grid (Ted Pella #01801, Redding, CA, USA), and dried at room temperature under atmospheric conditions. The size distributions of plasma-synthesized AgNPs were obtained by analysis with ImageJ software, v1.53s (National Institutes of Health, Bethesda, MD, USA). To confirm the surface plasmon resonance effects, absorption spectra of plasma-synthesized Ag@Cit NPs were measured by an optical absorption spectrometer (Maya2000Pro, Ocean Insight, Orlando, FL, USA).

Measurements of Dye Degradation by Plasma-Synthesized Silver Nanoparticles
A nonbiodegradable and commonly used organic dye, methylene blue (MB), was selected as a model analyte. MB and sodium borohydride (NaBH 4 ) were purchased from Sigma-Aldrich (Yongin-city, Kyunggi-do, Korea). To investigate the degradation activity of MB dye by plasma-synthesized Ag@Cit NPs, 0.3 mL of 1 mM MB was mixed with 10 mL DW, 0.3 mL of 100 mM NaBH 4 , and 1.0 mL of Ag@Cit NPs. For the dye degradation experiment, the plasma-synthesized Ag@Cit NP solutions were diluted 11.6-fold with DW. Consequently, solutions with 26 µM MB were treated by the plasma-synthesized Ag@Cit NPs. The Ag@Cit NP treatment duration was 10 min. Optical absorption spectra of Ag@Cit NP-treated and -untreated MB solutions were measured by an optical absorption spectrometer (Maya2000Pro, Ocean Insight, Orlando, FL, USA).
Next, we investigated dye degradation activity of plasma-synthesized Ag@Cit NPs coated onto filter paper to achieve dye degradation without remnant silver nanoparticles. The filter paper was immersed in a Ag@Cit NP solution for 3 h and dried at 60 • C for 1 h. After drying, most of the plasma-activated liquid was removed. In this research, Ag@Cit NPs loaded onto the filter paper is called Ag@Cit NP-paper. To examine dye degradation effects of the Ag@Cit NP-paper, Ag@Cit NP-coated and -uncoated papers were soaked in 26 µM MB solutions. After dye degradation treatment for 1 h in the Ag@Cit NP-paper-containing solution, pristine and Ag@Cit NP-paper-treated MB solutions were transferred into a 15 mL conical tube and used for further experiments.

Optical Properties of the Plasma Jet
Optical emission spectroscopy is a common, fast, simple, and straightforward method to investigate the various reactive and energized species in the plasma jet. Optical emission spectra (OES) of the J1, J2, and J3 jets in the 200-1000 nm region displayed the characteristic emission peaks of plasma-induced reactive oxygen and nitrogen species, as shown in Figure 4a. The emission lines in the 700-900 nm region are attributed to 2p → 1s transition of excited argon molecules [35]. Intense emission at 309 nm corresponds to the hydroxyl radical (OH) produced by breakdown of water molecules present in ambient air under the influence of electrons and metastable atoms [36,37]. At the plasma-liquid interface, prolonged plasma exposure causes a dissociation of water molecules by energetic ions from the plasma jet, resulting in OH radical generation. Weak emission bands in the 330-380 nm range are generated from the second positive system transition of nitrogen molecules (C 3 Π u → B 3 Π g ) [37,38], which can be ascribed to plasma operation under atmospheric conditions. The emission at 777 nm was attributed to 2s 2 2p 3 ( 4 S 0 )3s → 2s 2 2p 3 ( 4 S 0 )3p transition of atomic oxygen (O) [39]. The emission line at 656 nm was attributed to Balmer H α transition of excited hydrogen atoms (Figure 4b). The Balmer emission indicates that hydrogen atoms were produced in the plasma-synthesized AgNO 3 -TSC-DW. Under our experimental conditions, most of the hydrogen atoms were produced from the plasma electron reduction of hydrogen ions that came from citrate (TSC). As shown in Figure 4b, the hydrogen emission intensity was stronger in the J2 and J3 jets than the J1 jet, which was attributed to the higher concentration of plasma electrons in the longer jet. Meanwhile, the hydrogen emission intensity of the J3 jet was lower than the expectation from the plasma electron-induced reduction of hydrogen ions. The hydrogen emission intensity of the J3 jet was slightly higher than that of the J2 jet. Hydrogen generation by plasma electron reduction of hydrogen ions may compete with other product generation processes by reaction of hydrogen atoms with plasma-induced reactive species (hydroxyl radical, hydroperoxyl radical, etc.). Next, the J1 jet produced more reactive nitrogen species (RNS) than the longer jet (Figure 4a). Only a small fraction of nitrogen molecules can be dissociated in the outflow of the longer jet due to the lower electron temperature. Electron excitation and ionization of nitrogen molecules are more favorable in the J1 jet because the shorter jet exhibited a higher electron temperature than the longer jet ( Figure 3). The binding of plasma-induced nitrate (nitrite) with hydrogen ions led to acidic water and prevents H + -to-H conversion, leading to the suppression of the plasma synthesis of AgNPs in the J1 jet. Overall, the longer jet generated a larger number of hydrogen atoms and a smaller amount of RNS in the AgNO 3 -TSC-DW.

Electrical Conductivity and Acidity of Plasma-Treated Silver Precursor Solutions
Electrical conductivity and pH are key parameters governing the physicochemical properties of a plasma-activated medium. Atmospheric-pressure plasma in contact with an aqueous solution produces aqua electrons, resulting in the transport of electrons from the gaseous plasma into the aqueous solution [40,41]. Aqua electrons can affect the electrical conductivity and acidity of plasma-treated silver precursor solution. To study the plasma synthesis mechanism of AgNPs, we measured the conductivies and pH values of plasma-treated silver precursor solutions. As shown in Figure 5, the electrical conductivies of the plasma-treated-DW and AgNO 3 -TSC-DW increased significantly with plasma treatment duration. When DW was treated with the plasma jet for 10 min, the electrical conductivies of plasma-treated-DW rose from 0 µS/cm to 63, 99, and 107 µS/cm for the J1, J2, and J3 jets, respectively (Figure 5a). The electrical conductivies of plasma-treated AgNO 3 -TSC-DW jumped from 970 µS/cm to 1044, 1085, and 1093 µS/cm for the J1, J2, and J3 jets, respectively, (Figure 5b). The plasma-untreated AgNO 3 -TSC-DW showed electrical conductivity of 970 µS/cm. During plasma treatment, plasma-generated aqua electrons accumulated in the plasma-treated AgNO 3 -TSC-DW. As shown in Figure 5c, AgNO 3 and TSC contribute to the electrical conductivity of the AgNO 3 -TSC-DW. Plasma treatment for AgNO 3 -DW, TSC-DW, and AgNO 3 -TSC-DW increased their electrical conductivies due to plasma-generated aqua electrons. By subtracting the electrical conductivity of plasma-untreated AgNO 3 -TSC-DW (σ c ) from the electrical conductivity of the plasmatreated AgNO 3 -TSC-DW (σ p ), we obtained the electrical conductivity of plasma-generated aqua electrons (σ e(aq) = σ pσ c ). For a plasma treatment duration of 10 min, the electrical conductivities of plasma-generated aqua electrons were estimated to be 74, 115, and 123 µS/cm for the J1, J2, and J3 jets, respectively, as shown in Figure 6. The concentration of plasma-induced aqua electrons can be estimated from their electrical conductivity: n e(aq) = σ e(aq) /(e · µ e ). Here, n e(aq) , e, and µ e are the concentration, charge, and mobility of aqua electrons in the plasma-treated-AgNO 3 -TSC-DW, respectively. From the reported value (µ e = 1.84 × 10 -3 cm 2 /(V · s)) for the mobility of aqua electrons [42], we can estimate the aqua electron density of plasma-activated medium. For a plasma treatment duration of 10 min, the aqua electron densities of plasma-treated AgNO 3 -TSC-DW increased to 2.5 × 10 21 /m 3 , 3.9 × 10 21 /m 3 , and 4.2 × 10 21 /m 3 for the J1, J2 and J3 jets, respectively, as shown in Figure 6. The aqua electron density of the longer jet was higher than that of the shorter jet.  When DW was treated for 10 min with the plasma jets, the pH values of plasmatreated-DW dropped from 7.0 to 4.5, 4.7, and 5.0 for the J1, J2, and J3 jets, respectively, as shown in Figure 7a. Prolonged plasma treatment of DW produced a lower pH. Plasma treatment of DW produced acidic water due to the plasma-induced hydrogen ion-nitrate (nitrite) complex formation. When TSC-DW was treated with the plasma jets for 10 min, the pH values of plasma-treated-TSC-DW were 6.8, 7.1, and 7.2 for the J1, J2, and J3 jets, respectively, (Figure 7c). The pH values of plasma-treated-TSC-DW are higher than those of plasma-treated-DW, indicating that TSC can scavenge plasma-generated hydrogen ions. For a plasma exposure duration of 10 min, the pH values of plasma-treated AgNO 3 -TSC-DW increased slightly to 7.3 to 7.5, 7.9, and 7.6 for the J1, J2, and J3 jets, respectively ( Figure 7b). As described in the following section, plasma-assisted citrate reduction of silver ions can be facilitated at higher pH values.

Structural and Optical Properties of Plasma-Synthesized Silver Nanoparticles
After the AgNO 3 -TSC-DW was treated with the plasma jet for a few minutes, the colors of the silver precursor solutions changed from colorless to yellow (Figure 8b), indicating that Ag@Cit NPs were successfully generated by plasma-assisted citrate reduction. Absorption spectra of the plasma-synthesized Ag@Cit NPs exhibited surface plasmon resonance (SPR) bands at approximately 406 nm ( Figure 8a). As the plasma treatment duration increased from 2 min to 10 min, absorption coefficients of Ag@Cit NP solutions at the SPR peaks increased from 0.67/cm, 1.2/cm, and 0.75/cm to 4.0/cm, 8.6/cm, and 5.8/cm for the J1, J2 and J3 jets, respectively, (Figure 8c), indicating that prolonged plasma treatment produced a larger amount of Ag@Cit NPs. This happened because prolonged plasma treatment of silver precursor solutions led to more plasma reductants to be used for synthesis of Ag@Cit NPs. Regarding the effect of plasma plume length on the synthesis of Ag@Cit NPs, the SPR peak intensity of Ag@Cit NPs was stronger in the J2 jet than the J1 and J3 jets, indicating that the J2 jet can synthesize a larger amount of Ag@Cit NPs than the shorter and longer jets. For a plasma treatment duration of 10 min, the SPR wavelengths of Ag@Cit NPs were 405.9, 405.0, and 406.9 nm for the J1, J2, and J3 jets, respectively, indicating similar average particle sizes of plasma-synthesized Ag@Cit NPs. This is because TSC stabilizes Ag@Cit NPs in an aqueous solution by suppressing their growth.  Figure 9a shows the HR-TEM images of the Ag@Cit NPs synthesized by treating AgNO 3 -TSC-DW for 10 min with the J1, J2, and J3 jets. The Ag@Cit NPs by the J2 jet exhibited a single size distribution that could fit to Gaussian and Lorentzian functions, but the Ag@Cit NPs by the J1 and J3 jets caused two size distributions: one smaller and one larger. Considering that the Ag@Cit NPs by the J1, J2, and J3 jets exhibited a single SPR peak at approximately 406 nm, larger nanoparticles might be produced from the aggregation of nanoparticles during the drop casting of nanoparticles on the TEM grid. In the HR-TEM images of the Ag@Cit NPs by the J1 and J3 jets, smaller nanoparticles correspond to plasmasynthesized Ag@Cit NPs. Plasma-synthesized Ag@Cit NPs featured a quasi-spherical shape with average particle diameters of 7.5 ± 2.1, 6.6 ± 2.0, and 5.9 ± 2.1 nm for the J1, J2, and J3 jets, respectively, (Figure 9b). Figure 9c

Plasma Synthesis Routes of Silver Nanoparticles
The plasma reduction routes of silver ion (Ag + ) to zero-valent silver (Ag 0 ) nanoparticles are well established, and are based on the two following reactions [5,23,43]: Ag + + H Ag 0 + H + .
The reaction in Equation (1) is the reduction of silver ions by plasma electrons that are solvated into the aqueous solution. The main source of plasma electrons to reduce silver ions to AgNPs is the excited argon atoms. Plasma-excited argon atoms can provide plasma electrons that are required to reduce silver ions to AgNPs. The standard reduction potential of the silver ion is 0.80 V, which can instantly capture plasma electrons to form AgNPs. Equation (2) shows the reduction of silver ions through hydrogen atoms formed in the aqueous solution due to plasma-liquid interactions. Meanwhile, a large amount of hydrogen ions can digest silver nanoparticles (Equation (2)), leading to a smaller amount of AgNPs, as shown in Figure 8d. Plasma consists of charged particles (electrons and ions), neutral particles, and UV light. When the plasma jet enters the aqueous solution, plasma radicals in gas phase can be transported to solution, and these solvated reactive species change the physicochemical properties of plasma-treated water. In the plasma-treated-DW and AgNO 3 -TSC-DW, additional reactive species can be generated through the following plasma-liquid interactions [44][45][46][47][48][49]: e - * + H 2 O → H + + OH -+ eaq , H + + O -2 HO 2 , pK a = 4.8, H + + eaq → H, Here, e and eaq are dry (non-hydrated) and aqua (hydrated) electrons, respectively, and M * is any plasma ion with an ionization energy above the H 2 O ionization threshold. In the plasma-treated DW, hydrogen ions were generated through plasma-induced UV photolysis (Equation (6)), and/or electrolysis (Equation (7)) of water molecules, and then the formation of the nitrate (nitrite)-hydrogen ion complex results in acidic water (Equations (17) and (19)), as shown in Figure 7a. For plasma treatment of the TSC-DW, plasma-generated hydrogen ions were captured by citrate (Figure 7c). Similarly to the plasma-treated TSC-DW, the plasma-treated AgNO 3 -TSC-DW exhibited higher pH values (Figure 7b). Now we demonstrate the effects of citrate on the silver nanoparticle synthesis. Under higher pH conditions, Ag@Cit NPs can be efficiently synthesized from AgNO 3 and TSC (Na 3 C 6 H 5 O 7 = Na 3 Cit) [50,51]: H 3 Cit H 2 Cit -+ H + , pK a = 3.13, H 2 Cit -HCit 2-+ H + , pK a = 4.76, HCit 2-Cit 3-+ H + , pK a = 6.40.
Here, Cit 3-= (C 6 H 5 O 7 ) 3is the citrate trianion. Citric acid (C 6 H 8 O 7 = H 3 Cit) can be oxidized to citrate, and the oxidation products of citric acid depend on pH [52]. Under neutral or basic conditions (6 ≤ pH ≤ 11), the dominant structure of oxidized citric acid is a citrate trianion (C 6 H 5 O 7 = Cit 3-). In the plasma-treated AgNO 3 -TSC-DW, plasma-generated aqua electrons convert hydrogen ions to hydrogen atoms (Equation (11)). Hydrogen-induced reduction of silver ions is the dominant process for the plasma synthesis of Ag@Cit NPs because the reduction potential of the hydrogen ion is lower than that of the silver ion. In the plasma-treated AgNO 3 -TSC-DW, silver nanopartcles were produced from the reduction of silver ions through hydrogen atoms, and then a silver nanoparticle-citrate complex (Ag@Cit NPs) was formed [13].
Next, we consider the effects of plasma plume length on the plasma-synthesized silver nanoparticles. Experimentally, plasma treatment of the AgNO 3 -DW did not produce a sufficient amount of silver nanoparticles, whereas that of AgNO 3 -TSC-DW did. These results indicate that plasma electron-induced reduction of silver ions by reaction Equation (1) is not main synthesis route of silver nanoparticles under our experimental conditions. Next, citrate reduction of AgNO 3 -TSC-DW without boiling or plasma treatment failed to produce a sufficient amount of silver nanoparticles. Significant amounts of silver nanoparticles could be synthesized only when the plasma jet was applied to AgNO 3 -TSC-DW. Plasmaassisted citrate reduction of silver ions is more effective than citrate reduction of silver ions without plasma. Considering that reduction of silver ions by plasma electrons in the AgNO 3 -DW did not produce a sufficient amount of silver nanoparticles, the reduction of silver ions in AgNO 3 -TSC-DW is attributed to hydrogen atoms from the citrate (Equations (11) and (20)-(23)). In the plasma-treated AgNO 3 -TSC-DW, hydrogen-induced reduction of silver ions is the main synthesis route of silver nanoparticles. Efficient synthesis of silver nanoparticles can be achieved by citrate reduction of silver salt under neutral or basic conditions (6 ≤ pH ≤ 11) (Equations (21)-(25)) [51,53]. The synergetic effect between citrate and plasma electrons plays an important role in the plasma-synthesis of silver nanoparticles. The amount of plasma-synthesized Ag@Cit NPs was determined by the hydrogen atoms that were produced from the plasma electron-induced reduction of hydrogen ions. As shown in Figure 8c, longer-duration plasma treatment produced a larger amount of Ag@Cit NPs, because prolonged plasma treatment of AgNO 3 -TSC-DW led to more plasma electrons to be used for synthesis of Ag@Cit NPs. Regarding the effect of plasma plume length on the synthesis of silver nanoparticles, the amount of plasmasynthesized Ag@Cit NPs was largest in the J2 jet ( Figure 8a). As shown in Figure 5b, the electrical conductivity was higher in the longer jets than in the J1 jet. The electrical conductivity of the J2 jet was significantly higher than that of the J1 jet, and the electrical conductivity of the J3 jet was slightly higher than that of the J2 jet. The longer jet produced higher electrical conductivity, indicating that more plasma electrons were generated in the longer jet ( Figure 6). Meanwhile, the pH value of the J2 jet was higher than those of the shorter and longer jets, as shown in Figure 7b. That is, the J2 jet led to the highest pH value of the AgNO 3 -TSC-DW.
To further study the effect of plasma plume length on the synthesis of Ag@Cit NPs, we consider the relationship between the amount of plasma-synthesized silver nanoparticles and the acidity of the plasma-treated medium. From the silver nanoparticle synthesis reaction (Equation (2)) for reduction of silver ions through hydrogen atoms, = K eq , at equilibrium.
Here, K eq is the equilibrium constant of the reaction (Equation (2) At a fixed concentration of silver ions, a higher hydrogen atom concentration can produce a higher silver nanoparticle concentration, but a higher hydrogen ion concentration can digest silver nanoparticles. In the J1 and J3 jets, a lower pH indicates a larger number of hydrogen ions, which can convert AgNPs to silver ions, leading to a smaller number of Ag@Cit NPs. To summarize, the largest amount of Ag@Cit NPs obtained in the J2 jet is attributed to a higher pH and a larger number of aqua electrons. Plasma-assisted citrate reduction of silver ions is the main synthesis route of Ag@Cit NPs.
In addition, citrate facilitates the conversion of silver ions to Ag@Cit NPs and stabilizes Ag@Cit NPs in an aqueous solution. Plasma-synthesized Ag@Cit NPs are well-dispersed in an aqueous solution. The formation of stabilized nanoparticles is due to electrostatic repulsion of citrate-capped AgNPs, as confirmed by the zeta potential of -55 mV.

Degradation Activity of Methylene Blue Dye Treated by Plasma-Synthesized Silver Nanoparticles
To confirm potential applications of the Ag@Cit NPs for the degradation of environmental pollutants, we investigated the degradation activity of plasma-synthesized Ag@Cit NPs on MB dye. The degradation activity of Ag@Cit NP treatment on MB was measured by optical absorption spectroscopy [54]. Figure 10a exhibits the absorption spectra of Ag@Cit NP-treated and -untreated MB solutions. The corresponding photos of Ag@Cit NPtreated MB solutions are displayed in Figure 10c. The pristine MB solution exhibited a broad absorption band with a strong peak at 670 nm and a shoulder at 615 nm [55]. The 670 nm absorption can be assigned to the MB monomer, and the 615 nm absorption can be attributed to the MB dimer. Comparing the Ag@Cit NP-treated MB with the pristine MB, the 670 nm absorption peak of MB disappered by the Ag@Cit NP treatment. However, the Ag@Cit NP-treated MB exhibited significant absorption at 670 nm. To confirm the degradation activity of Ag@Cit NPs on MB, we measured the degradation activity of the nanoparticle-free plasma-activated medium (PAM). As positive controls of the plasmasynthesized Ag@Cit NPs, the TSC-DW solutions were treated with the J1, J2, and J3 jets for 10 min. As shown in Figure 10b, the 670 nm absorption intensity of PAM-treated-MB was similar to that of pristine MB, indicating that the degradation activity of Ag@Cit NPs is stronger than that of PAM. The nonzero absorption of Ag@Cit NP-treated MB at 670 nm may be ascribed to dye degradation products induced from the interaction of MB with Ag@Cit NPs. One possible explanation for the formation of dye degradation products by the Ag@Cit NP treatment is AgNP-induced electron transfer from BH -4 ions to MB dye [56]. To fully understand the dye degradation mechanism by the Ag@Cit NP treatment, it will be necessary to conduct a more detailed study for exploring the dye degradation performance of Ag@Cit NPs and plasma-activated medium. (f) Photos of Ag@Cit NP-paper-treated MB. Ag@Cit NPs were synthesized by treating AgNO 3 -TSC-DW for 10 min with the J1, J2, and J3 jets. Nanoparticle-free plasma-activated media were prepared by treating TSC-DW for 10 min with the J1, J2, and J3 jets. Solutions with 26 µM MB were treated by plasma-synthesized Ag@Cit NPs. Figure 10d exhibits the absorption spectra of Ag@Cit NP-paper-treated MB solutions. The corresponding photos of Ag@Cit NP-paper-treated MB solutions are displayed in Figure 10f. Comparing the 670 nm absorption peak of Ag@Cit NP-paper-treated MB with that of pristine MB, the 670 nm absorption peak of Ag@Cit NP-paper-treated MB decreased to almost zero, as shown in Figure 10e. Absorption spectra of the Ag@Cit NP-papertreated MB solutions do not have an SPR absorption band of Ag@Cit NPs, indicating that degradation of MB dye without remnant silver nanoparticles was achieved by the Ag@Cit NP-paper treatment.

Conclusions
Citrate-capped silver nanoparticles were synthesized by treating AgNO 3 -TSC-DW with atmospheric-pressure argon plasma jets. Ag@Cit NPs with an average particle diameter of about 5.9-7.5 nm were obtained after plasma treatment for 10 min. Plasma-synthesized Ag@Cit NPs have a face-centered cubic structure, and their absorption spectra exhibited surface plasmon resonance peaks at approximately 406 nm. Citrate facilitated the conversion of silver ions to Ag@Cit NPs and stabilized AgNPs in an aqueous solution. Plasma-synthesized Ag@Cit NPs were well-dispersed in the aqueous solution. The amount of Ag@Cit NPs increased in a plasma-treatment duration-dependent manner. The acidity and electrical conductivity measurements showed that a larger amount of Ag@Cit NPs were produced from the 8.5 cm plume jet with a higher pH and a larger number of aqua electrons. Plasma synthesis of Ag@Cit NPs was more effective in the 8.5 cm plume jet than in the shorter and longer plume jets. These results indicate that the synergetic effect between plasma electrons and citrate played an important role in the plasma synthesis of Ag@Cit NPs. Plasma-synthesized Ag@Cit NPs exhibited significant dye degradation activity for methylene blue.