Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing Development

Novel nanoarchitectures based on lignin nanoparticles (LNPs) were designed and realized for electrochemical eco-friendly biosensing development. Two types of lignin nanoparticles were utilized for the modification of a gold bare electrode, namely organosolv (OLNPs) and kraft lignin (KLNPs) nanoparticles, synthetized from a sulfur-free and a sulfur lignin, respectively. The electrochemical behavior of LNP-modified electrodes was studied using two electrochemical techniques, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Compared to the gold bare electrode, an evident decrease in the faradaic current and increase of the ΔEp were observed in cyclic voltammograms. In addition, larger semicircles were registered in Nyquist plots. These results suggest a strong inhibition effect of the electron transfer reaction by LNPs layer, especially in the case of KLNPs. The modified electrodes, properly assembled with concanavalin A (ConA) and glucose oxidase (GOx), were successively tested as biosensing platforms for glucose, showing a sensitivity of (4.53 ± 0.467) and (13.74 ± 1.84) μA mM−1 cm2 for Au/SAMCys/OLNPs/ConA/GOx and Au/KLNPs/ConA/GOx biosensors, respectively. Finally, different layers of the KNLPs/ConA/GOx-modified Au electrode were tested, and the three-layered Au(KNLPs/ConA/GOx)3 showed the best analytical performance.


Introduction
In the last decade, significant effort has been devoted to the design and development of biosensors with improved characteristics in terms of selectivity, sensitivity, simplicity and response time. One of the most critical issues in biosensing design is the achievement of high sensitivity and selectivity together with an easy-to use format. To this aim, the choice of a proper biosensing interface is fundamental.
Several nanomaterials have been successfully used as electrochemical platforms for the immobilization of bioreceptor molecules with the desired orientation, allowing the retention of their biological activity. In addition to the immobilization step, the nanoplatforms perform as an analyte recognition site and a signal transduction and amplification probe for the enhancement of biosensor performances [1][2][3][4][5][6]. The nanoarchitecture in biosensor design is expected to offer high selectivity, accuracy and fast and real-time responsiveness, which are the main challenges in the current stage of biosensor development.
A variety of nanostructured materials have been utilized in the construction of biosensors, such as carbon-based materials like carbon nanotubes [7][8][9][10][11][12][13][14][15] and graphene [16][17][18][19][20][21], metal nanoparticles [22][23][24], quantum dots [25], nanowires [26,27] and magnetic nanoparticles [28]. Despite the extraordinary electrochemical properties of the nanobiosensors and their possibility of miniaturization for point-of-care (POC) use, drawbacks for clinical applications need to be solved. Among them, labeling and sample pretreatment steps require high technical experience for operation and preclude real-time analysis. Other limitations are associated with the biological affinity probes rather than in the actual electrical detection mechanism. Therefore, a better understanding of the immobilization chemistry and minimizing nonspecific binding are crucial issues in the development of biosensing devices.
In the last decade, lignin nanoparticles (LNPs) [29][30][31][32] have attracted a great deal of attention because of their biotechnological applications in different fields, including in antibacterial [33], antioxidant [34,35] and UV-adsorbent [36] agents, hybrid nanocomposites [37], drug delivery systems [38][39][40] and biocatalysis [41,42]. LNPs show novel characteristics such as a higher surface area, increased UV-adsorption capacity and radical scavenger activity compared to native counterparts. Lignin, the most abundant polyphenol in nature, is a polymer produced by the radical coupling of three phenylpropanoid units, as shown in Scheme 1 [43]. It is the only polymer in nature that contains such a large number of aromatic compounds, responsible for the beneficial properties associated with its aromatic character. Every year, approximately 50 million tons of lignin are produced worldwide as byproducts of the pulp and paper industry and biorefinery. Therefore, it is emerging as a new economic renewable raw material in a wide range of applications. During the lignin nanostructuration process, supramolecular π-interactions occur between the aromatic moieties, thus favoring electron transfer processes able to increase the electrochemical responsiveness. In particular, LNPs have been used as renewable and sustainable platforms for the immobilization of redox enzymes, such as laccase [44], tyrosinase [45] and the enzymatic cascade of horseradish peroxidase (HRP) and glucose oxidase (GOx) [46]. The benign effect of the ordered immobilization of the two enzymes on the surface of LNPs has been reported, focusing on the role of concanavalin A (Con A), a lectin able to interact with the carbohydrate component of both glycoproteins HRP and GOx [46], in the increase in the activity of the system [47,48].
There are two types of lignins based on different extraction processes: sulfur lignin, produced by chemical pulping processes, including kraft lignin (KL) and ligno-sulfonates (LSs), and sulfur-free lignin, including alkaline lignin and organosolv lignin (OL), produced by bioethanol production processes [49]. OL and KL differ in their elemental composition, molecular weight and purity, the main difference being the presence of sulfur in KL (Scheme 1). KL is soluble in water while OL is insoluble in water and soluble in organic solvents [50]. KL and OL nanoparticles have been used for innovative applications and advanced materials [51][52][53][54].
In this work, we describe and compare the use of organosolv lignin nanoparticles (OLNPs) and kraft lignin nanoparticles (KLNPs) as novel nanoarchitectures for the modification of a bare gold electrode for electrochemical biosensor development. In particular, we discuss the preparation and the electrochemical characterization of LNPs-modified platforms and, as a proof-of-concept, their application in the development of a glucose biosensor. The electrode was assembled by the layer-by-layer (LbL) procedure, and the electrochemical and biocatalytic electrode performance was evaluated depending on the number of deposited layers, as well as on the role played by Con A in the orientation of the GOx immobilization. To the best of our knowledge, no reports are present in literature on electrode platforms based on LNPs-assembled multilayer films composed by LNPs/ConA/GOx for glucose detection.

Reagents and Apparatus
Glucose oxidase from Aspergillus niger (EC 1.10.3.2, 160 kDa; catalytic activity according to producer specification is 100-250 U per mg) and Con A from jack beans (Canavalia ensiformis) type VI were purchased from Sigma-Aldrich, Buchs, Switzerland, and stored at −18 °C.

Reagents and Apparatus
Glucose oxidase from Aspergillus niger (EC 1.10.3.2, 160 kDa; catalytic activity according to producer specification is 100-250 U per mg) and Con A from jack beans (Canavalia ensiformis) type VI were purchased from Sigma-Aldrich, Buchs, Switzerland, and stored at −18 • C.
Lignin nanoparticles from organosolv lignin (OL) or kraft lignin (KL) were prepared according to nanoprecipitation procedures described in our previous works [47]. Briefly, a solution of OL or KL (2.0 mg in 1.2 mL of THF/H 2 O 5:1 v/v) was added to Milli-Q water (in 3.8 mL) to obtain OLNPs and KLNPs.
Electrochemical impedance (EIS) was utilized to characterize the different modifications of the Au electrode surface. EIS measurements were carried out at an equilibrium potential called the open circuit potential (OCP) without bias voltage in the frequency range of 0.1-10 3 Hz using an ac signal of 10 mV amplitude at a formal potential of the redox probe (0.22 V vs. Ag/AgCl), using Autolab Potentiostat/Galvanostat (Eco Chemie, The Netherlands). EIS measurements were carried out using 20 mL of PBS buffer solution containing a mixture of 5 mM Fe(CN) 6 3− / Fe(CN) 6 4− as an electrochemical probe.

Preparation of Gold-Modified Electrodes
The gold electrode (Au) was polished using polish paper and alumina powders, 0.3 and 0.05 µm, and sonicated in clean water, followed by electrocycling in 0.5 M H 2 SO 4 from −0.3 to +1.7 V vs. Ag/AgCl, for 20 scans at 0.3 Vs −1 and from −0.3 to +1.7 V vs. Ag/AgCl, for 2 scans at 0.1 Vs −1 .
The OLNP-modified biosensor was assembled by the following method: firstly, the cysteamine monolayer was prepared by soaking the clean gold electrodes (Au) in 18 mM cysteamine (CA) aqueous solution for 4 h at room temperature (RT) in darkness, washing the electrode thoroughly with water to remove physically adsorbed cysteamine. The carboxyl functions on the surface of organosolv lignin nanoparticles (OLNPs, 10 µL 2 mg mL −1 ) were activated with 20 µL of a mixture containing 0.5 mM EDC and 0.1 mM NHS in water for about 20 min. After removing EDC-NHS mixture and rinsing the surface of the electrode with PBS buffer solution, 20 µL of ConA (1 mg mL −1 ) was dropped on the working electrode. The immobilization of GOx was carried out by dropping 5 µL of a 5 mg mL −1 GOx solution (prepared in PBS buffer solution). Finally, the modified electrode (Au/SAMCys/OLNPs/GOx) was exhaustively rinsed with PBS buffer solution before electrochemical measurements (Scheme 2a).

Layer-by-Layer Nanoassembly
Two and three layers of the KNLPs/ConA/GOx biolayer were successively deposited onto the Au electrode according to a self-assembly LbL procedure (Scheme 3), as reported above for the first layer. The KLNPs biosensor (Au/KLNPs/GOx) was prepared by immersing the clean bare gold electrode in a solution of kraft lignin nanoparticles (KLNPs, 2 mg mL −1 ) for 2 h in darkness at room temperature. Then, ConA and GOx enzyme were added as described above for the first biosensor (Scheme 2b).

Layer-by-Layer Nanoassembly
Two and three layers of the KNLPs/ConA/GOx biolayer were successively deposited onto the Au electrode according to a self-assembly LbL procedure (Scheme 3), as reported above for the first layer.

Layer-by-Layer Nanoassembly
Two and three layers of the KNLPs/ConA/GOx biolayer were successively deposited onto the Au electrode according to a self-assembly LbL procedure (Scheme 3), as reported above for the first layer.

SEM Experiments
A high-resolution field emission scanning electron microscopy (SEM) (HR FESEM, Zeiss Auriga Microscopy, Jena, Germany) was used to investigate the morphology of the electrodes. All samples were prepared using gold plates (25 Å~25 Å~1 mm, ALS Co., Ltd., Tokyo, Japan) instead of gold electrodes.

SEM Characterization
The surface morphology of OLNPs-and KLNPs-modified electrodes was studied using scanning electrode microscopy (SEM) experiments. Figure 1 shows the SEM images relative to the OLNPs (Panel A) and KNLPs (Panel B) Au-modified electrode, and a simple Au bare electrode (Panel C), as reference, at two different magnifications (50,000 and 5000× magnification). Regular spherical colloid particles with no significant aggregates or clusters for both types of lignin nanoparticles were observed. OLNPs result to be smaller than KLNPs with an average diameter between 150 and 200 nm, about half of that measured for KLNPs (average diameter 300-450 nm) (

Electrochemical Characterization
The electrochemical behavior of the LNPs-modified electrodes was studied using two electrochemical techniques, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), able to characterize the reversibility of the electron transfer process

Electrochemical Characterization
The electrochemical behavior of the LNPs-modified electrodes was studied using two electrochemical techniques, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), able to characterize the reversibility of the electron transfer process and the charge transfer resistance at the electrode-solution interface, respectively. The redox couple Fe(CN) 6 3−/4− was used as an anionic electrochemical probe for examining the different platforms on a gold classical electrode [24]. Figure 2 shows the CV profile obtained before (black curve) and after the modification of a gold bare electrode with OLNPs (Au/SAMCys/OLNPs, blue curve) and KNLPs (Au/KLNPs, red curve) at a potential scan rate of 100 mV s −1 . However, the presence of aromatic rings in the lignin molecules can somehow facilitate the ET due to the delocalized π-electrons, as confirmed by the two-peak shaped voltammograms (blue and red curves).

Cyclic Voltammetry Characterization
The decrease in the faradaic current and the increase of the ΔEp values can also be interpreted by the electrostatic repulsive interactions between the negatively charged Fe(CN)6 3−/4− and the LNPs, which show a negative Z potential value (−42 mV at pH 4.2) [56]. In order to better understand the extent of this effect, a comparison between the voltammetric responses of the commonly used negatively charged potassium ferricyanide and a positively charged redox probe, exaammineruthenium(III), was carried out and the results are reported in Figure 3. As it can be clearly observed, the negative effect in terms of peak currents and ΔEp exerted by the KNLPs layer is present only with ferricyanide. When exammineruthenium(III) is used, the peak currents were almost unaffected by the lignin biolayer with a lower increase in the peak current separation. These results can be attributed to the anionic character of the lignin biolayer, which creates strong repulsive interactions with the negative ferricyanide, not present in the case of the cationic redox probe. Moreover, [Ru(NH3)6] 3+ is known to be insensitive to surface modification within monolayer thickness as it is able to exchange electrons by tunneling [57]. KLNPs possess anchoring thiol groups in their structure, which allow the formation of direct S-Au bonds with the electrode. In the case of ONLPs, it was necessary to use a cysteamine self-assembled monolayer (SAMCys) to allow the connection to the surface of the gold electrode. Measurements of OLNPs directly adsorbed onto an Au electrode without the use of a SAM were previously carried out, and the voltammetric curves resulted to be stackable to the CVs obtained with the Au bare electrode (data not shown), because of the clearly visible leakage of the OLNPs in the cell solution during repeated scan cycles.
All voltammograms show a couple of redox peaks with a decrease in the peak current and an increase in the peak-to-peak distance after electrode modification with both ONLPs and KNLPs. The shape of the voltametric curve is similar for the three curves, indicating that the electron transfer (ET) process is under diffusion control. Further, the modification of the electrode surface leads to much lower peak current values and larger current separations between forward and reverse scan, suggesting a possible inhibition of the ET reaction caused by a "blocking behavior" exerted by the LNPs, which can enhance the insulating properties of the electrode without passivating its surface. This effect is more marked with KLNPs compared to SAMCys/OLNPs, most likely due to the formation of a more homogeneous KNLPs biolayer which almost completely covers the electrode surface. In the case of SAMcys/OLNPs, the presence of the spacer increases the distance between LNPs and the electrode surface, causing an increase in the electron tunneling, and at the same time, a decrease in the loading of LNPs on the electrode surface.
However, the presence of aromatic rings in the lignin molecules can somehow facilitate the ET due to the delocalized π-electrons, as confirmed by the two-peak shaped voltammograms (blue and red curves).
The decrease in the faradaic current and the increase of the ∆E p values can also be interpreted by the electrostatic repulsive interactions between the negatively charged Fe(CN) 6 3−/4− and the LNPs, which show a negative Z potential value (−42 mV at pH 4.2) [56]. In order to better understand the extent of this effect, a comparison between the voltammetric responses of the commonly used negatively charged potassium ferricyanide and a positively charged redox probe, exaammineruthenium(III), was carried out and the results are reported in Figure 3. As it can be clearly observed, the negative effect in terms of peak currents and ∆E p exerted by the KNLPs layer is present only with ferricyanide. When exammineruthenium(III) is used, the peak currents were almost unaffected by the lignin biolayer with a lower increase in the peak current separation. These results can be attributed to the anionic character of the lignin biolayer, which creates strong repulsive interactions with the negative ferricyanide, not present in the case of the cationic redox probe. Moreover, [Ru(NH 3 ) 6 ] 3+ is known to be insensitive to surface modification within monolayer thickness as it is able to exchange electrons by tunneling [57].  Table 1 shows the electroactive area (Ae, mm 2 ), the heterogeneous electron transfer rate constant (k 0 , cm s −1 ) and the roughness factor (ρ) of the bare and the two modified electrodes. The Ae has been evaluated using the Randles-Ševcik equation [58], k 0 was calculated using an extended method obtained by merging Klingler-Kochi and Nicholson-  Table 1 shows the electroactive area (A e , mm 2 ), the heterogeneous electron transfer rate constant (k 0 , cm s −1 ) and the roughness factor (ρ) of the bare and the two modified electrodes. The A e has been evaluated using the Randles-Ševcik equation [58], k 0 was calculated using an extended method obtained by merging Klingler-Kochi and Nicholson-Shain methods, for totally irreversible and reversible systems, respectively [59], and the roughness factor (ρ) derives from the ratio of the electroactive (A e ) to the geometric area (A g ). Table 1. Comparison of electrochemical parameters before and after the modification of Au electrode: electroactive area (A e , mm 2 ), electron transfer rate constant (k 0 , cm s −1 ) and roughness factor (ρ). Geometric area (A g , mm 2 ) = 7.06.

Electrochemical
Platform A e (mm 2 ) k 0 (10 −3 cm s −1 ) ρ The Randles-Ševcik equation for a reversible process is as follows: I p = 2.686 × 10 5 n 3/2 A e D 0 1/2 C 0 v 1/2 (1) where I p is the voltametric peak current (A), n is number of electrons, A e is electroactive area (cm 2 ), D 0 is diffusion coefficient (7.6 × 10 −6 cm 2 s −1 for ferricyanide), C 0 is the concentration (mol cm −3 ) and v is the scan rate (Vs −1 ). By using the slope of the plot of I p vs. v 1/2 , the electroactive areas were calculated and the results are shown in Table 1. The A e values of Au/SAMcys/OLNPs and Au/KLNPs resulted to be about 2-and 7-times smaller than that of the bare electrode, respectively. The coverage of the electrode surface with the non-conductive LNPs results in an obvious decrease of the A e values, with a marked effect reported for the KNLPs, as explained above. Consequently, ρ values also decreased with electrode modifications because of the presence of the nanoparticle monolayer as the active surface ( Table 1). The heterogeneous electron transfer rate constant (k 0 ) for the Au bare is much higher than those for the Au-modified sensors; however, the k 0 value obtained with Au/SAMcys/ OLPNs electrode is more than one order of magnitude higher than that obtained with KLNPs, most likely due to its particular nanoarchitecture realized by the SAM molecules, which allow an electron-tunneling effect through the layer.

Electrochemical Impedance Spectroscopy Characterization
EIS is one of the very sensitive techniques used for characterization of surfaces and for detecting biomolecular interactions occurring at the surface of an electrode [25][26][27]. Figure 4 shows the Nyquist plots for the bare and the two modified Au electrodes in the presence of 5 mM Fe(CN) 6 3− / 4− redox couple. The impedance measurements were carried out at formal potential of 0.22 V vs. Ag/AgCl. The impedance data were simulated by different equivalent circuits, as shown in the Figure 4 inset. A Randles circuit was successfully applied to fit the Au bare electrode (Figure 4, inset (a)). The obtained simulation shows low resistance of charge transfer (Rct = 725 ± 1 Ω cm 2 ) suggesting a fast charge transfer between the redox probe and electrode.
After SAM formation and the amino-amidic bonding with OLNPs, the layer became thicker and the reaction slowed, and another equivalent circuit was proposed ( Figure 4, inset b). The Rct value increased to 1.83 ± 0.03 kΩ cm 2 , confirming the occurred modification by blocking of the interfacial charge transfer. Finally, the equivalent circuit fitting procedure applied to the impedance data of KNLP-modified electrode showed a very large Rct value, as expected (Rct = 15.4 ± 0.5 kΩ cm 2 ).
These results are in good agreement with the results obtained by cyclic voltammetry discussed in the previous paragraph.

Catalytic Behavior of Au/SAMCys/OLNPs/Con A/GOx and Au/KLNPs/Con A/GOx Platforms
The glycoenzyme GOx, a well-studied system, was chosen as a model enzyme for studying the bioelectrocatalytic features of the two modified LNP electrodes, assembled with Con A as biosensing platforms. In order to confirm the enzymatic activity of the immobilized GOx, the electrochemical re-oxidation of an osmium complex (OsCmplx) was followed in the mediated oxidation of the FADH2 group of GOx and the subsequent oxidation of glucose. The Os-redox polymer was chosen because of its cationic character, which does not create negative repulsions with the negative lignin biolayer, showing a similar voltammogram (curve not shown) to that obtained in the case of [Ru(NH3)6]Cl3 ( Figure 3, curve b), and at the same time because it is suitable to be used with the function The bare Au electrode exhibits a very small semicircle at a high frequency region and a low frequency straight line, indicating a reversible and diffusion-controlled ET process of the redox probe ( Figure 4, blue curve).
On the contrary, lignin nanoparticle modified electrodes exhibit much larger semicircle formations because of the inhibition of the ET by the formation of the nano-lignin biolayer. In particular, it can be seen from Figure 4 that the SAMCys/OSLNP-modified electrode shows the formation of a semicircle in the high frequency region and a small straight line in the low frequency region (black curve), highlighting a quasi-reversible behavior of the redox probe and confirming a moderate blocking capacity of the biolayer. In the case of KNPLs, a very large semicircle over the entire range of frequencies used is observed, attesting a stronger blocking behavior and a high inhibition of the redox reaction.
The impedance data were simulated by different equivalent circuits, as shown in the Figure 4 inset. A Randles circuit was successfully applied to fit the Au bare electrode (Figure 4, inset (a)). The obtained simulation shows low resistance of charge transfer (R ct = 725 ± 1 Ω cm 2 ) suggesting a fast charge transfer between the redox probe and electrode.
After SAM formation and the amino-amidic bonding with OLNPs, the layer became thicker and the reaction slowed, and another equivalent circuit was proposed (Figure 4, inset  b). The R ct value increased to 1.83 ± 0.03 kΩ cm 2 , confirming the occurred modification by blocking of the interfacial charge transfer. Finally, the equivalent circuit fitting procedure applied to the impedance data of KNLP-modified electrode showed a very large R ct value, as expected (R ct = 15.4 ± 0.5 kΩ cm 2 ).
These results are in good agreement with the results obtained by cyclic voltammetry discussed in the previous paragraph.

Catalytic Behavior of Au/SAMCys/OLNPs/Con A/GOx and Au/KLNPs/Con A/GOx Platforms
The glycoenzyme GOx, a well-studied system, was chosen as a model enzyme for studying the bioelectrocatalytic features of the two modified LNP electrodes, assembled with Con A as biosensing platforms. In order to confirm the enzymatic activity of the immobilized GOx, the electrochemical re-oxidation of an osmium complex (OsCmplx) was followed in the mediated oxidation of the FADH 2 group of GOx and the subsequent oxidation of glucose. The Os-redox polymer was chosen because of its cationic character, which does not create negative repulsions with the negative lignin biolayer, showing a similar voltammogram (curve not shown) to that obtained in the case of [Ru(NH 3 ) 6 ]Cl 3 ( Figure 3, curve b), and at the same time because it is suitable to be used with the function of both mediator and support for many different redox enzymes [60]. Typical voltammograms ( Figure 5, Panels A and B) demonstrated the catalytic activity of GOx assembled by Con A on LNP-modified electrodes, using OsCmplx as a redox mediator. The presence of Con A on the electrode increased the anodic current with a concomitant disappearance of the cathodic peak (S-shape), according to an electrocatalytic process. The resulting calibration plots are shown in Figure 5C (Panel C).
The kinetic and analytical parameters of the two proposed platforms are summarized in Table 2. A linear relation between the current response of the electrode and the amount of glucose in the concentration range 0.33-2.5 and 0.15-2.5 mM was obtained for Au/SAMCys/OLNPs/Con A/GOx and Au/KLNPs-SH/Con A/GOx biosensors, respectively. At higher concentrations, the amperometric response is no longer linear due to the saturation of the active site of the enzyme. The detection limit of the biosensors were found to be 0.11 and 0.05 mM, respectively, calculated using the relation 3σ/S, where σ is the absolute standard deviation of the intercept and S is the slope of the calibration curve [61]. The sensitivity resulted to be three times higher with KNLPs than OLNPs. This result, apparently in contrast with data previously reported, can be explained by taking into account the larger electrode coating of the KNLPs layer, which allows an immobilization of a much larger amount of enzyme. Moreover, the presence of Con A favors a correct orientation of the GOx enzyme during the immobilization process [47], enhancing the electrocatalytic performance of the system.
The apparent Michaelis-Menten constants, obtained by fitting the calibration curves, show a reduction of the K M app value with KNLPs, suggesting a larger enzymatic affinity for glucose when KNLPs are used.
The best electrode platform was Au/KLNPs/Con A/GOx, and therefore this platform was used for further experiments.
of both mediator and support for many different redox enzymes [60]. Typical voltammograms ( Figure 5, Panels A and B) demonstrated the catalytic activity of GOx assembled by Con A on LNP-modified electrodes, using OsCmplx as a redox mediator. The presence of Con A on the electrode increased the anodic current with a concomitant disappearance of the cathodic peak (S-shape), according to an electrocatalytic process. The resulting calibration plots are shown in Figure 5C (Panel C).

LbL Functionalization of the Au/KLNPs/Con A/GOx Platform
Different layers of the KNLPs/CoA/GOx were assembled by repeating the steps described for the preparation of the Au-modified electrode (Section 2.2.1). The properties of the resulting glucose biosensor were measured by cyclic voltammetry. The amperometric current uniformly increases at increasing number of layers from one to three (Figure 6), reaching a saturation after the third layer (data not shown).

Conclusions
In this work, a study on the use of organosolv and kraft lignin nanoparticles for the design and development of novel nanoassembled electrochemical platforms is presented. The data reported that the properties of the nanoparticles are governed by the type of lignin used. A blocking behavior was shown by both LNP-gold-modified electrodes because of the insulating nature of the LNPs. This effect is more pronounced with KLNPs compared to OLNPs, most likely because of the formation of a thicker KNLPs layer, which allows a better coating of the electrode surface. In addition, OLNPs were anchored to the Au electrode surface through a SAM molecule, which increases the distance between the LNPs and the electrode surface, with a consequent lower loading of the nonconductive LNPs and enhancement of the electron-tunneling effect through the layer.
The Au/KLNPs/Con A/GOx platform showed better electrocatalytic results compared to Au/SAMCys/OLNPs/Con A/GOx due to the efficient coating of KNLPs, which allows an immobilization of a larger amount of enzyme. Moreover, the presence of the lectin Con A favored the correct orientation of the GOx enzyme during the immobilization process, contributing to the enhancement of the electrocatalytic performance of the system. A self-assembly-driven modification strategy consisting of immobilizing three layers of KNLPs/CoA/GOx allowed the construction of a nanostructured film with the maximum catalytic current. This result confirms that LbL technique is a powerful tool to create functional nanocoatings with enhanced properties. These findings hold great application prospects for future applications of lignin nanoparticles in eco-friendly biosensor developments. Acknowledgments: The authors acknowledge the EU project-EASME/EMFF/Blue Economy-2018/n.863697 "FISH chitinolytic biowastes FOR FISH active and sustainable packaging material" Comparing the voltammograms of the three-layers system, a two-times increase in the current signal was observed between the first and the third layer. Thus, the assembling of the three layers exhibited a remarkable improvement on the catalytic properties in the recognition of glucose, confirming that the LbL assembly process is able to enhance the performances of the biosensors in terms of sensitivity, reproducibility and stability [62,63]. In particular, the three-layered Au(KNLPs/CoA/GOx) 3 biosensor showed an enhanced sensitivity of 15.85 µA/mM cm 2 , a linear range between 0.1 and 2.5 mM and a lower LOD of 0.02 mM, compared to the one-layered same platform. In addition, the increase in the current value clearly demonstrates the important role played by Con A, which allows a well-oriented immobilization of GOx and by LNPs due to their boosting effect in the activity of the overall electrocatalytic system, as previously hypothesized in our previous work [47].

Conclusions
In this work, a study on the use of organosolv and kraft lignin nanoparticles for the design and development of novel nanoassembled electrochemical platforms is presented. The data reported that the properties of the nanoparticles are governed by the type of lignin used. A blocking behavior was shown by both LNP-gold-modified electrodes because of the insulating nature of the LNPs. This effect is more pronounced with KLNPs compared to OLNPs, most likely because of the formation of a thicker KNLPs layer, which allows a better coating of the electrode surface. In addition, OLNPs were anchored to the Au electrode surface through a SAM molecule, which increases the distance between the LNPs and the electrode surface, with a consequent lower loading of the nonconductive LNPs and enhancement of the electron-tunneling effect through the layer.
The Au/KLNPs/Con A/GOx platform showed better electrocatalytic results compared to Au/SAMCys/OLNPs/Con A/GOx due to the efficient coating of KNLPs, which allows an immobilization of a larger amount of enzyme. Moreover, the presence of the lectin Con A favored the correct orientation of the GOx enzyme during the immobilization process, contributing to the enhancement of the electrocatalytic performance of the system. A self-assembly-driven modification strategy consisting of immobilizing three layers of KNLPs/CoA/GOx allowed the construction of a nanostructured film with the maximum catalytic current. This result confirms that LbL technique is a powerful tool to create functional nanocoatings with enhanced properties. These findings hold great application prospects for future applications of lignin nanoparticles in eco-friendly biosensor developments.