Supported Gold Nanoparticles as Catalysts in Peroxidative and Aerobic Oxidation of 1-Phenylethanol under Mild Conditions

The efficiency of Au/TiO2 based catalysts in 1-phenylethanol oxidation was investigated. The role of support modifiers (La2O3 or CeO2), influence of gold loading (0.5% or 4%) and redox pretreatment atmosphere, catalyst recyclability, effect of oxidant: tert-butyl hydroperoxide (TBHP) or O2, as well as the optimization of experimental parameters of the reaction conditions in the oxidation of this alcohol were studied and compared with previous studies on 1-octanol oxidation. Samples were characterized by temperature-programmed oxygen desorption (O2-TPD) method. X-ray photoelectron spectroscopy (XPS) measurements were carried out for used catalysts to find out the reason for deactivation in 1-phenylethanol oxidation. The best catalytic characteristics were shown by catalysts modified with La2O3, regardless of the alcohol and the type of oxidant. When O2 was used, the catalysts with 0.5% Au, after oxidative pretreatment, showed the highest activity in both reactions. The most active catalysts in 1-phenylethanol oxidation with TBHP were those with 4% Au and the H2 treatment, while under the same reaction conditions, 0.5% Au and O2 treatment were beneficial in 1-octanol oxidation. Despite the different chemical nature of the substrates, it seems likely that Au+(Auδ+) act as the active sites in both oxidative reactions. Density functional theory (DFT) simulations confirmed that the gold cationic sites play an essential role in 1-phenylethanol adsorption.


Introduction
Alcohol oxidation is one of the most important transformations in industrial organic chemistry and a challenging process in terms of green chemistry [1,2]. Traditional methods involve the use of toxic, expensive, stoichiometric metal oxidants and harmful organic solvents, and often require harsh reaction conditions [3,4]. Catalysis research is working towards favourable solutions to these problems through the development of effective heterogeneous catalysts for environment friendly applications.
One example is the development and study of catalytic systems for the oxidation of 1-phenylethanol, which is an aromatic alcohol with high reactivity. The main oxidation product of its oxidation is acetophenone (methylphenylketone, C 6 H 5 COCH 3 ). Acetophenone is used in perfumes, soaps and creams, as well as a flavouring substance in food, soft drinks and tobacco. It is also used as a solvent and has a sleeping effect, important in the manufacture of medicines.
There are many studies reported for 1-phenylethanol oxidation, using both homogeneous and heterogeneous catalysis (Table 1). From the summarized results, it can be concluded that the catalytic oxidation of this alcohol, a representative of cyclic alcohols, can be carried out under mild conditions: using green oxidizing agents such as oxygen, air and peroxides, both with a solvent and in its absence, in moderate temperature ranges: from room temperature to 150 • C and, in most cases, at atmospheric pressure. Also, when using peroxides, a new trend is the reaction under the influence of microwave heating, which, in comparison with the traditional process, allows obtaining high yields in a short time. Some disadvantages exist for homogeneous catalysts, namely the inability to reuse them, and the need for addition of bases and radicals, which researchers often apply to increase the yield of acetophenone. However, an exception is the work where some of us were able to synthesize catalysts based on iron complexes with low solubility, which were efficiently reused [9].
When heterogeneous base metals catalysts were used, selective oxidation of 1-phenylethanol at room temperature was possible, however, a large catalyst load and a long reaction time up to 24 h were required [13]. Catalysts based on palladium, silver, and ruthenium, exceeded the activity of previous catalyst systems, as expected, and were not deactivated during the recycling tests [16,17]. However, the disadvantages in the case of silver and palladium catalysts would include the formation of by-products [16,18,19], and a high ratio of ruthenium to alcohol needed when using ruthenium catalysts [20].
Gold-containing systems have been extensively investigated in this process [19,[21][22][23][24][25][26][27][28][29][30][31][32]. The main feature of these systems is their high activity and selectivity; however, there is a tendency to their gradual deactivation due to an increase in the size of gold nanoparticles (Au NPs) during reaction and recycling tests. Also, in most cases, the authors suggest that highly dispersed gold in the metallic state is responsible for the excellent activity in the oxidation of 1-phenylethanol [24,26,27,29,31]. However, there are also supporters of the cationic nature of gold active centres in this process [23,28,33] and Liang et al. [25] considered negative charged gold as an active site.
Unfortunately, there are very few works in which the mechanism of both aerobic and especially peroxidative oxidation of 1-phenylethanol is proposed. Furthermore, when using TBHP as the oxidizing agent, the role of the catalyst is attributed to the decomposition of this oxidant into radicals responsible for the direct oxidation of alcohol [34,35]. In general, it can be concluded that supported gold-containing systems are effective catalysts in the oxidation of 1-phenylethanol; however, even in the oxidation of such a reactive alcohol, the study of the mechanism of formation of the active surface of gold-containing systems responsible for excellent catalytic performance is still the subject of numerous discussions.
Previously, in a series of our works [36][37][38][39], it was shown that 1-octanol, a representative of the less reactive alcohols, whose physical properties impose constraints to implementation of green chemistry approaches, could be effectively oxidized under mild conditions and using Au/(Me x O y )/TiO 2 catalysts. It was found that the formation of an active surface responsible for the catalytic properties is strictly dependent on the modifying additives used for improving metal-support interaction, a way to transform and stabilize positively charged gold active sites. It would be expected that such catalysts would exhibit higher catalytic activity in the oxidation of more reactive alcohols, such a 1-phenylethanol, which lead us to carry out the present study. Moreover, this work should also allow comparing the catalytic behaviours towards different types of primary alcohols, one inactivated (alkyl) and another activated (bearing an aromatic ring), represented by 1-octanol and 1-phenylethanol, respectively. Therefore, in this work, we continued to investigate Au/TiO 2 based catalysts, unmodified and modified with lanthana and ceria, aiming to assess their performance in 1-phenylethanol oxidation under mild conditions. We also wanted to compare the results with those previously obtained for 1-octanol oxidation, using the same catalysts, to find out whether the nature of the active sites of catalysts in 1-phenylethanol oxidation is the same as in 1-octanol, depending on a number of factors: the influence of the nature of the oxidizing agent, support nature, the pretreatment atmosphere and gold content. Additionally, to find a theoretical conclusion about the effect of gold cationic sites on alcohol activation, DFT simulations were performed.

Materials and Methods
Titania P25 (nonporous, 70% anatase and 30% rutile, particle size: 21 nm, purity: 99.5%, Evonik Degussa GmbH, (Essen, Germany) was used as the starting support and was modified by impregnation with aqueous solutions of La(NO 3 ) 3 ·6H 2 O or Ce(NO 3 ) 3 ·6H 2 O (Merck, Darmstadt, Germany) in a molar ratio Ti/Me (La, Ce) = 40. Au was loaded on the supports with a nominal loading of 0.5 and 4 wt.% using HAuCl 4 ·3H 2 O (Merck, Darmstadt, Germany) as precursor, by deposition-precipitation with urea (Merck, Darmstadt, Germany) in the absence of light following the previously reported procedure [40,41]. Briefly, the gold precursor (4.2 × 10 −3 M) and urea (0.42 M) were dissolved in distilled water, and thereafter the support was added to the solution. The resulting mixture was heated to 80 • C and kept at constant temperature for 16 h, with stirring. The initial pH was 2.4. The pH was adjusted to 7.5 by the end of gold deposition. After the deposition-precipitation procedure, the samples were centrifuged (11,000 rpm, 15 min), washed with water then centrifuged four times, and dried under vacuum 2 h at 80 • C. After drying, the samples were stored at room temperature in a desiccator under vacuum, away from light, in order to prevent any alteration.
In this work, temperature-programmed oxygen desorption (O 2 -TPD) was used to assess the nature of the interaction of oxygen with the surface of the catalyst and the support. All experiments were performed on a "Chemosorb" chemisorption analyzer (Neosib, Novosibirsk, Russia) equipped with a thermal conductivity detector (TCD), which was calibrated with O 2 prior analysis. A sample (0.2 g) pretreated at 300 • C in helium flow (60 mL/min) was saturated with oxygen at 40 • C for 1 h. Oxygen desorption was carried out in a helium stream from 40 to 650 • C with a heating rate of 20 • C/min.
The used (i.e., after the reaction test) catalysts were also characterized by X-ray photoelectron spectroscopy. Therefore, the surface composition and the chemical state of gold were determined by XPS analysis, performed on an ESCALAB 200A spectrometer (VG Scientific, Waltham, MA, USA) using Al Kα radiation (1486.6 eV). A pass-energy of 40 eV and a step size of 0.1 eV/step were selected. The charge effect was corrected using the C1s peak as a reference (binding energy of 285 eV). The CASA XPS software (version 2.3.15, CASA Software Ltd, Teignmouth, UK, http://www.casaxps.com/) was used for data analysis.
The catalytic properties for 1-phenylethanol oxidation were studied using catalyst samples either without treatment or after pre-treatment at 300 • C in H 2 or O 2 atmosphere. Therefore, catalysts samples will be denoted hereinafter as n% Au/(Me x O y )/TiO 2 _X, where n is the gold content in wt.%, Me is the modifier metal (La or Ce) and X indicates the applied pretreatment atmosphere (O 2 or H 2 ). The catalytic tests were carried out in a batch reactor operated under atmospheric conditions at the temperature 80 • C for 6 h. The stirring reaction mixture was as follows: 1.27 µmol of Au were Nanomaterials 2020, 10, 151 6 of 23 introduced in 1-phenylethanol (6 mmol) as the substrate, TBHP (70% v/v aqueous solution, 14.6 mmol) as the oxidizing agent, in a base-and solvent-free medium.
After the reaction test, the mixture was allowed to cool down to room temperature. Typically, to conduct the product analysis, 10 µL of benzaldehyde (internal standard) and 1 mL of MeCN were added to 100 µL of such reaction mixture. The resulting sample was centrifuged for 15 min and analyzed by gas chromatography (GC) using the internal standard method. Blank tests indicated that only traces (4%) of ketone were generated. Chromatographic analyses were undertaken using a GC 8000 series gas chromatograph (Fisons Instruments, Loughborough, UK) equipped with a BP-20 (WAX) capillary column (SGE Analytical Science Europe Ltd, Milton Keynes, UK) and a flame ionization detector (FID) detector (Fisons Instruments, Loughborough, UK). Molar yield (%) was defined based on substrate, i.e., moles of product per 100 mol of substrate, determined by GC. Attribution of peaks was made by comparison with chromatograms of genuine samples and, in some cases, by gas chromatography mass-spectrometry (GC-MS) analyses with He as the carrier gas using a Clarus 600C instrument (Perkin Elmer, Waltham, MA, USA), equipped with a 30 m × 0.22 mm × 25 µm BPX5 (SGE Analytical Science Europe Ltd, Milton Keynes, UK) capillary column. According to the GC-MS acetophenone was the only product in both aerobic and peroxidative oxidation of 1-phenylethanol.
To adequately compare the catalytic results of aerobic oxidation of 1-phenylethanol with those of 1-octanol, the conditions selected for the oxidation of 1-phenylethanol with molecular oxygen were as follows: alcohol/gold ratio (R) = 100 mol/mol, 25 mL of a 0.1 M solution of 1-phenylethanol in mesitylene, O 2 flow = 30 mL/min, stirring 800 rpm. And to compare the catalytic results of the peroxidative oxidation of 1-octanol with of those of 1-phenylethanol, the conditions selected for the peroxidative oxidation of 1-octanol were as follows: R = 5000 mol/mol, T = 80 • C, TBHP/alcohol = 2.43 mmol/mmol, stirring 800 rpm, without solvent or base added. In the absence of support/catalyst in the reaction mixture no activity was observed in both aerobic and peroxidative oxidation of 1-octanol.
To perform the recycling experiments, the used sample was separated from the reaction mixture by centrifugation (5000 rpm, 15 min) and decantation, washed 4 times with 5 mL of acetonitrile and dried at 50 • C to constant weight. It was then reused for the oxidation test as described above.
The adsorption of 1-phenylethanol on gold nanoparticles was simulated in a scalar-relativistic approach using the density functional (DFT) method PBE [44]. The tetrahedral Au 20 and Au 20 + clusters are considered as models of gold nanoparticles. The Au 20 cluster has been obtained experimentally [45] and is a popular model for studying structural effects in catalysis [46,47]. Because the cluster has atoms located at the top, on the facet and on the edge and having different coordination number, we can effectively apply this model to study the structural effects in the adsorption of phenylethanol. A cationic gold cluster was obtained by removing one electron from Au 20 with subsequent optimization of the structure. The effect of the support was ignored as a first approximation. The interaction of 1-phenylethanol or 2-phenylethanol with Au 20 cluster was simulated: Different coordination modes of alcohol on Au 20 by the OH group or the phenyl fragment were considered. The structures of (OH-CH 2 -CH 2 -Ph)Au 20 isomers were optimized, and the total energies of the reagents and products were calculated taking into account the energy of zero vibrations. The change in total energy and standard enthalpies of the reaction (1) were determined according to the formula: The calculations were performed in the PRIRODA program (version 17, Russia) [48] using a Lomonosov supercomputer [49].

Peroxidative Oxidation of 1-Phenylethanol
Since we had the goal of finding out the same active sites in both processes, the reaction conditions should be the same. Therefore, as in the case of 1-octanol oxidation, all experiments on both peroxidative and aerobic oxidation of 1-phenylethanol were carried out at a temperature of 80 • C (conventional heating) (Scheme 1). Nanomaterials 2019, 9, x FOR PEER REVIEW 7 of 23 The calculations were performed in the PRIRODA program (version 17, Russia) [48] using a Lomonosov supercomputer [49].

Peroxidative Oxidation of 1-Phenylethanol
Since we had the goal of finding out the same active sites in both processes, the reaction conditions should be the same. Therefore, as in the case of 1-octanol oxidation, all experiments on both peroxidative and aerobic oxidation of 1-phenylethanol were carried out at a temperature of 80 °C (conventional heating) (Scheme 1).

Scheme 1. Oxidation of 1-phenylethanol over Au/(MexOy)/TiO2 catalysts.
We started the catalytic experiments with TBHP as oxidizing agent with a very low catalyst loading equal to the ratio of 1-phenylethanol/Au of 5000 and in no-solvent conditions. The results are presented in Table 2. As expected, both unmodified and modified catalysts proved to be much more active in the oxidation of 1-phenylethanol than in that of 1-octanol. Taking into account the high alcohol/gold ratio, the catalysts, even in the as-prepared state, reached more than 50% of acetophenone yield after 6 h of reaction, despite the low activity in the first hours (Scheme 1). As in the oxidation of 1-octanol [38,39], the sample modified with lanthanum oxide demonstrated the best catalytic performance ( Table 2): 98% yield of acetophenone was achieved already in 1 h when 4% Au/La2O3/TiO2 pretreated in H2 was used. It should also be noted that the activity of the studied catalysts increased with increasing gold content. We started the catalytic experiments with TBHP as oxidizing agent with a very low catalyst loading equal to the ratio of 1-phenylethanol/Au of 5000 and in no-solvent conditions. The results are presented in Table 2. As expected, both unmodified and modified catalysts proved to be much more active in the oxidation of 1-phenylethanol than in that of 1-octanol. Taking into account the high alcohol/gold ratio, the catalysts, even in the as-prepared state, reached more than 50% of acetophenone yield after 6 h of reaction, despite the low activity in the first hours (Scheme 1). As in the oxidation of 1-octanol [38,39], the sample modified with lanthanum oxide demonstrated the best catalytic performance ( Table 2): 98% yield of acetophenone was achieved already in 1 h when 4% Au/La 2 O 3 /TiO 2 pretreated in H 2 was used. It should also be noted that the activity of the studied catalysts increased with increasing gold content. However, it should be emphasized that the supports also showed some activity under these conditions, as the yield of acetophenone increased with time and depended on the support nature (Entries 2-4, Table 2). In the absence of a catalyst or support, the formation of a small amount of acetophenone was also observed (Entry 1, Table 2). For the oxidation of 1-phenylethanol with molecular oxygen, no activity was observed when using supports only, and neither in the absence of a support/catalyst, as will be seen below. This shows the very good activity of TBHP, which, when decomposed by heating or by reaction with a metal (see Introduction), forms radicals (t-BuO·, t-BuOO·), responsible for the direct oxidation of alcohols [34]. Therefore, in the referred work, 97% acetophenone was obtained using 6 equivalents of TBHP at 100 • C in 24 h, without adding any catalysts or bases [35].
When using the La-modified sample, which showed a medium activity in 1-phenylethanol peroxidative oxidation, direct dependence of the yield of acetophenone on catalyst loading was observed (Table 3). Thus, 100% acetophenone was reached already after 15 min of reaction when the total gold amount was increased from 1.27 to 10 µmol. It should be noted that experiments using hydrogen peroxide (30% aqueous solution) as oxidant were not effective. In the case of the 4% Au/La 2 O 3 /TiO 2 _H 2 catalyst, only 11% of the product yield was achieved after 6 h of reaction, under the same conditions used with TBHP (T = 80 • C, 6 mmol 1-phenylethanol, 14.6 mmol H 2 O 2 , 1.27 µmol of Au). With the same catalyst, a 98% yield of acetophenone was obtained after 1 h using TBHP (Entry 20, Table 2). Thus, conditions close to the principles of green chemistry were selected to compare with the above-mentioned oxidation process of 1-octanol: a solvent-free oxidation of 1-phenylethanol with Au/(Me x O y )/TiO 2 catalysts, for 6 h, at T = 80 • C, using a minimum gold loading (1.27 µmol), and TBHP as a green oxidant, without any additives.

Aerobic Oxidation of 1-Phenylethanol
After replacing TBHP with molecular oxygen, keeping the same alcohol/gold ratio (R = 5000), we did not observe any conversion, even after 6 h of reaction (Table 4). The next step was to investigate the effect of the alcohol/gold ratio. With a ten-fold increase in catalyst loading (R = 500), 50% conversion was observed after 6 h of reaction. A complete conversion of 1-phenylethanol, using this catalyst, could be achieved only at R = 100, with 98% acetophenone yield being reached after just 30 min of reaction.
Such a different behaviour in the catalytic activity probably lies in the different oxidative capacity of oxygen and TBHP. Thus, the catalytic activity in the aerobic oxidation of 1-phenylethanol of the remaining catalysts was studied with R = 100. As it can be seen in Table 5, catalysts with a low gold content with an oxidative pretreatment were the most active, as in the case of 1-octanol (Figure 1b,e,f) [39].

Peroxidative and Aerobic Oxidation of 1-Octanol
Since this work also aimed at making a comparison with previous results obtained for the oxidation of 1-octanol, we also studied the effects of the oxidizing agent in the oxidation of this substrate. The trends of catalytic behaviour of the catalysts in the peroxidative and aerobic oxidation of 1-octanol were similar in terms of activity ( Figure 1). The most active catalysts were those with low Au loading, after an oxidative pretreatment. Regarding selectivity, it is important to note that when using molecular oxygen as an oxidizing agent, a different behaviour is observed in the distribution of oxidation products, depending on the pretreatment, the nature of the support and the gold content [39]. However, when TBHP was used as oxidizing agent, in the absence of a solvent, the main product, in all cases, was acid with a small amount of ester (up to 20%). This is probably due to the peroxide used as oxidizing agent which decomposed producing water [34], that is needed for octanoic acid formation, according to the Scheme 2 (route A) from [36,39]. The next step was to investigate the effect of the alcohol/gold ratio. With a ten-fold increase in catalyst loading (R = 500), 50% conversion was observed after 6 h of reaction. A complete conversion of 1-phenylethanol, using this catalyst, could be achieved only at R = 100, with 98% acetophenone yield being reached after just 30 min of reaction.
Such a different behaviour in the catalytic activity probably lies in the different oxidative capacity of oxygen and TBHP. Thus, the catalytic activity in the aerobic oxidation of 1-phenylethanol of the remaining catalysts was studied with R = 100. As it can be seen in Table 5, catalysts with a low gold content with an oxidative pretreatment were the most active, as in the case of 1-octanol (Figure 1b,e,f) [39].

Peroxidative and Aerobic Oxidation of 1-Octanol
Since this work also aimed at making a comparison with previous results obtained for the oxidation of 1-octanol, we also studied the effects of the oxidizing agent in the oxidation of this substrate. The trends of catalytic behaviour of the catalysts in the peroxidative and aerobic oxidation of 1-octanol were similar in terms of activity ( Figure 1). The most active catalysts were those with low Au loading, after an oxidative pretreatment. Regarding selectivity, it is important to note that when using molecular oxygen as an oxidizing agent, a different behaviour is observed in the distribution of oxidation products, depending on the pretreatment, the nature of the support and the gold content [39]. However, when TBHP was used as oxidizing agent, in the absence of a solvent, the main product, in all cases, was acid with a small amount of ester (up to 20%). This is probably due to the peroxide used as oxidizing agent which decomposed producing water [34], that is needed for octanoic acid formation, according to the Scheme 2 (route A) from [36,39]. Scheme 2. Possible reaction pathways for the oxidation of 1-octanol on supported gold catalysts (Adapted from [36,39]). Therefore, according to all catalytic results, under comparable reaction conditions, 1-phenylethanol can be much more efficiently and selectively oxidized over Au/Me x O y /TiO 2 catalysts than 1-octanol. Moreover, the best results in the oxidation of both alcohols were achieved using TBHP.
In all cases, the best catalytic characteristics were shown by catalysts modified with lanthanum oxide, regardless of the alcohol and the type of oxidizing agent. Also, when a solvent was used and molecular oxygen was present as an oxidizing agent, the catalysts with the lowest gold content after oxidative pretreatment showed the highest activity in both 1-phenylethanol and 1-octanoloxidation.
The only difference was that under no-solvent peroxidative conditions, the most active catalysts in 1-phenylethanol oxidation are those with a high load of gold and the hydrogen treatment, while under the same reaction conditions, low gold loading and oxygen treatment were beneficial in 1-octanol oxidation.

Catalyst Characterization
Catalysts were previously characterized by several techniques [38,39,42,43]. The N 2 adsorption and EDX analysis ( Table 6) showed that the textural properties and the content of gold cannot be the reason for the different catalytic behaviour observed for the catalysts. In addition, TEM and STEM-HAADF measurements showed no direct correlations between the average Au particle size and catalytic properties. A number of nanoparticles in the range 1-7 nm with different distributions were observed in all catalysts (Table 6). Table 6. Textural properties of supports and catalysts, analytical content and particle size of Au. Adapted from [38,39]. XPS measurements of catalysts showed that gold formed different electronic states on the support surface-ions Au + and Au 3+ , and neutral gold nanoparticles. The ratio among these states depends strongly on the support and the modifier nature (Table 7). XPS results for ceria modified samples are presented in Figure A1.

Sample
According to the H 2 -TPR and CO 2 -TPD results, it was concluded that modification by La oxide favored the formation of very stable ionic species Au δ+ (0 < δ < 1) by their localization on the strong basic Lewis sites, formed by two-electron orbitals of the oxygen atom on the support surface (data not shown, obtained in [39]). The highest portion of such gold states was observed in the Au/La 2 O 3 /TiO 2 sample, while they were practically absent in unmodified and modified with ceria catalysts. Also, according to DRIFT CO ( Figure A2, Reproduced from Ref. [43] with permission from the Royal Society of Chemistry) and XPS data, the contribution of singly charged ions and their stability were maximum for the most active catalysts (4% Au/La 2 O 3 /TiO 2 _H 2 and 0.5% Au/La 2 O 3 /TiO 2 _O 2 ). Thus, although the amount of monovalent ions (determined by XPS) for some materials was comparable to the values found for lanthanum-modified catalysts, strong and stable gold ions were only found in lanthanum-modified samples, according to the applied DRIFT CO method [43].  for lanthanum-modified catalysts, strong and stable gold ions were only found in lanthanum-modified samples, according to the applied DRIFT CO method [43]. O2-TPD was used to assess the nature of the interaction of oxygen with the surface of the catalyst and the support. The O2-TPD profiles obtained for the catalysts and their corresponding supports (Figure 2) show the presence of several peaks of oxygen desorption corresponding to different forms of adsorbed oxygen. Moreover, all the catalysts showed a wide peak of oxygen desorption in the range of 50-350 °C, which may be due to the adsorption of O2 − on TiO2, according to [50]. It should be noted that pure titania showed three overlapping peaks (at 95, 205 and 292 °C) in this temperature range. After gold deposition on TiO2, a slight change in the shape of these peaks was observed. After titania was modified with ceria and lanthana, the shape and position of the wide peak in the low-temperature region changed, overlapping maxima were observed at 100, 190 and 265 °C for CeO2/TiO2 and at 110, 230 and 310 °C for La2O3/TiO2.
The deposition of gold on the ceria-modified support did not cause a significant change in the O2-TPD profiles. However, after the deposition of gold on the surface of lanthana-modified titania, high-temperature peaks appeared in the range of 400-600 °C, and catalysts with 4% Au had a noticeably high intensity, with peaks at 460 and 560 °C. These desorption peaks are likely to occur at higher temperatures and correspond to adsorbed O − on the surface of TiO2, as described in [50,51]. Addition of lanthana and gold effectively stimulate the dissociation of O2 to O − , which has a higher activity than the super-oxo form O2 − in the oxidation reaction [50,52,53]. Thus, these results support the previously established favorable features of doping titania with lanthana (formation and stabilization of single charged gold ions through their localization on strong basic sites of La2O3/TiO2). Besides they revealed another promoting role of modifying additives of lanthana: providing the most active type of oxygen for effective oxidation of alcohols.
When comparing the XPS and the catalytic results of the catalysts in the peroxidative and aerobic oxidation of 1-phenylethanol (Table 8), there is no apparent correlation. Nevertheless, the most active catalysts (4% Au/La2O3/TiO2_H2 and 0.5% Au/La2O3/TiO2_O2) show the largest contribution of monovalent gold ions (Entries 1,2, Table 8). Moreover, all the catalysts showed a wide peak of oxygen desorption in the range of 50-350 • C, which may be due to the adsorption of O 2 − on TiO 2 , according to [50]. It should be noted that pure titania showed three overlapping peaks (at 95, 205 and 292 • C) in this temperature range. After gold deposition on TiO 2 , a slight change in the shape of these peaks was observed. After titania was modified with ceria and lanthana, the shape and position of the wide peak in the low-temperature region changed, overlapping maxima were observed at 100, 190 and 265 • C for CeO 2 /TiO 2 and at 110, 230 and 310 • C for La 2 O 3 /TiO 2 . The deposition of gold on the ceria-modified support did not cause a significant change in the O 2 -TPD profiles. However, after the deposition of gold on the surface of lanthana-modified titania, high-temperature peaks appeared in the range of 400-600 • C, and catalysts with 4% Au had a noticeably high intensity, with peaks at 460 and 560 • C. These desorption peaks are likely to occur at higher temperatures and correspond to adsorbed O − on the surface of TiO 2 , as described in [50,51]. Addition of lanthana and gold effectively stimulate the dissociation of O 2 to O − , which has a higher activity than the super-oxo form O 2 − in the oxidation reaction [50,52,53]. Thus, these results support the previously established favorable features of doping titania with lanthana (formation and stabilization of single charged gold ions through their localization on strong basic sites of La 2 O 3 /TiO 2 ). Besides they revealed another promoting role of modifying additives of lanthana: providing the most active type of oxygen for effective oxidation of alcohols. When comparing the XPS and the catalytic results of the catalysts in the peroxidative and aerobic oxidation of 1-phenylethanol (Table 8), there is no apparent correlation. Nevertheless, the most active catalysts (4% Au/La 2 O 3 /TiO 2 _H 2 and 0.5% Au/La 2 O 3 /TiO 2 _O 2 ) show the largest contribution of monovalent gold ions (Entries 1,2, Table 8).

Catalyst Recycling Tests
The catalyst recyclability in the peroxidative oxidation of 1-phenylethanolwas investigated up to six consecutive cycles, as described in the Experimental part, with the best performing catalyst, i.e., 4% Au/La 2 O 3 /TiO 2 _H 2 . As can be seen in Figure 3, there was a gradual catalyst deactivation during the recycling tests. Particularly, in the second cycle, the catalyst maintained 90% of activity, whereas in the third cycle a loss of 23% of its initial activity was observed. Consecutive decreasing of activity stopped at the sixth cycle where the yield of acetophenone kept the same level as in the fifth cycle (58-59%). Nevertheless, high selectivity to acetophenone was preserved in each cycle.

Catalyst Recycling Tests
The catalyst recyclability in the peroxidative oxidation of 1-phenylethanolwas investigated up to six consecutive cycles, as described in the Experimental part, with the best performing catalyst, i.e., 4% Au/La2O3/TiO2_H2. As can be seen in Figure 3, there was a gradual catalyst deactivation during the recycling tests. Particularly, in the second cycle, the catalyst maintained 90% of activity, whereas in the third cycle a loss of 23% of its initial activity was observed. Consecutive decreasing of activity stopped at the sixth cycle where the yield of acetophenone kept the same level as in the fifth cycle (58-59%). Nevertheless, high selectivity to acetophenone was preserved in each cycle. To find out the cause of the observed catalyst deactivation, XPS analysis of the catalyst after the first and last (6th) cycle was performed ( Figure 4) and compared with the XPS results for the catalyst before reaction. As in the "fresh" catalyst, before reaction (Entry 1, Table 8), two electronic states of gold were found in the used catalysts: metallic (Au 0 ) with binding energy (BE) (Au4f7/2) 84.2 and single charged ions (Au + ) with BE (Au4f7/2) 85.2 eV in catalyst after the 1st cycle and Au 0 with BE (Au4f7/2) 84.1 eV and (Au + ) with BE (Au4f7/2) 85 eV [54][55][56][57] in catalyst after 6th cycles were detected. However, according to XPS measurements, the surface concentration of gold is different among the studied samples (Entries 1,3,4, Table 8). It can be seen from Table 8 that, with each cycle, loss of catalytic activity decreased proportionally to the contribution of monovalent gold. That can probably be the reason for deactivation, as also in the case of 1-octanol (see detailed information in Table A1 as well as discussion of previous results from [39]), since gold monovalent ions were the proposed active species for the reaction. Yield of acetophenone, % Catalytic cycle To find out the cause of the observed catalyst deactivation, XPS analysis of the catalyst after the first and last (6th) cycle was performed ( Figure 4) and compared with the XPS results for the catalyst before reaction. As in the "fresh" catalyst, before reaction (Entry 1, Table 8), two electronic states of gold were found in the used catalysts: metallic (Au 0 ) with binding energy (BE) (Au4f 7/2 ) 84.2 and single charged ions (Au + ) with BE (Au4f 7/2 ) 85.2 eV in catalyst after the 1st cycle and Au 0 with BE (Au4f 7/2 ) 84.1 eV and (Au + ) with BE (Au4f 7/2 ) 85 eV [54][55][56][57] in catalyst after 6th cycles were detected. However, according to XPS measurements, the surface concentration of gold is different among the studied samples (Entries 1,3,4, Table 8). It can be seen from Table 8 that, with each cycle, loss of catalytic activity decreased proportionally to the contribution of monovalent gold. That can probably be the reason for deactivation, as also in the case of 1-octanol (see detailed information in Table A1 as well as discussion of previous results from [39]), since gold monovalent ions were the proposed active species for the reaction.   Figure 1).

Quantum Chemical Simulation of the Alcohol Adsorption on a Gold Cluster
The purpose of this study was to understand the following issues relating to the nature of the active sites of the gold nanoparticles in the activation of phenylethanol at the atomic level: (i) Which phenylethanol coordination on a gold cluster is preferred (by OH-or C6H5groups)? (ii) How do the structural features of the catalyst surface, including availability of low coordinated gold atoms, affect the adsorption of the alcohol? (iii) What is the effect of gold cationic sites on alcohol activation?
The optimized structures of phenylethanol-Au20 complexes, in which the alcohol is coordinated to a gold atom by the OH group, are shown in Figure 5. The energy changes during the formation of the complexes and the corresponding standard enthalpies are collected in Table 9. According to the calculated data, the most favorable coordination during the interaction of phenylethanol with Au20 is carried out at the top of the cluster. Both 1-phenylethanol and 2-phenylethanol bind to the top gold atom with similar values of adsorption energy (57-60 kJ/mol). The binding energies of alcohol on the edge and facet gold atoms are significantly lower (considering 1-phenylethanol as an example).  Figure 1).

Quantum Chemical Simulation of the Alcohol Adsorption on a Gold Cluster
The purpose of this study was to understand the following issues relating to the nature of the active sites of the gold nanoparticles in the activation of phenylethanol at the atomic level: (i) Which phenylethanol coordination on a gold cluster is preferred (by OH-or C 6 H 5 -groups)? (ii) How do the structural features of the catalyst surface, including availability of low coordinated gold atoms, affect the adsorption of the alcohol? (iii) What is the effect of gold cationic sites on alcohol activation?
The optimized structures of phenylethanol-Au 20 complexes, in which the alcohol is coordinated to a gold atom by the OH group, are shown in Figure 5. The energy changes during the formation of the complexes and the corresponding standard enthalpies are collected in Table 9. According to the calculated data, the most favorable coordination during the interaction of phenylethanol with Au 20 is carried out at the top of the cluster. Both 1-phenylethanol and 2-phenylethanol bind to the top gold atom with similar values of adsorption energy (57-60 kJ/mol). The binding energies of alcohol on the edge and facet gold atoms are significantly lower (considering 1-phenylethanol as an example).   Figure 1).

Quantum Chemical Simulation of the Alcohol Adsorption on a Gold Cluster
The purpose of this study was to understand the following issues relating to the nature of the active sites of the gold nanoparticles in the activation of phenylethanol at the atomic level: (i) Which phenylethanol coordination on a gold cluster is preferred (by OH-or C6H5groups)? (ii) How do the structural features of the catalyst surface, including availability of low coordinated gold atoms, affect the adsorption of the alcohol? (iii) What is the effect of gold cationic sites on alcohol activation?
The optimized structures of phenylethanol-Au20 complexes, in which the alcohol is coordinated to a gold atom by the OH group, are shown in Figure 5. The energy changes during the formation of the complexes and the corresponding standard enthalpies are collected in Table 9. According to the calculated data, the most favorable coordination during the interaction of phenylethanol with Au20 is carried out at the top of the cluster. Both 1-phenylethanol and 2-phenylethanol bind to the top gold atom with similar values of adsorption energy (57-60 kJ/mol). The binding energies of alcohol on the edge and facet gold atoms are significantly lower (considering 1-phenylethanol as an example). When alcohol is coordinated on the cluster through a benzene fragment (Figure 6), the adsorption energies decrease at all sites of Au20, compared to OH-group coordination. Among two different ways of alcohol coordination (OH-or C6H5-), coordination with the OH-group is advantageous (Complex 2, Figure 5). In this case, low-coordinated gold atoms are the most active in the activation of alcohol.  When alcohol is coordinated on the cluster through a benzene fragment (Figure 6), the adsorption energies decrease at all sites of Au 20 , compared to OH-group coordination. Among two different ways of alcohol coordination (OH-or C 6 H 5 -), coordination with the OH-group is advantageous (Complex 2, Figure 5). In this case, low-coordinated gold atoms are the most active in the activation of alcohol.  When alcohol is coordinated on the cluster through a benzene fragment (Figure 6), the adsorption energies decrease at all sites of Au20, compared to OH-group coordination. Among two different ways of alcohol coordination (OH-or C6H5-), coordination with the OH-group is advantageous (Complex 2, Figure 5). In this case, low-coordinated gold atoms are the most active in the activation of alcohol.
Au 20 The optimized structures of phenylethanol-Au 20 + complexes have similar features with neutral phenylethanol-Au 20 . In contrast, the calculated energy changes in reaction 4 (Table 9) are larger than in reaction 1. The binding energy of phenylethanol to low-coordinated cationic gold atoms through the OH-group is significantly higher than on the neutral cluster. When 1-phenylethanol is coordinated by an aromatic fragment on Au 20 + , the adsorption energy increases and becomes almost the same as the coordination of alcohol with the OH group. Based on the study, it can be concluded that the cationic sites play an important role in phenylethanol adsorption.

Conclusions
From the above results, it could be concluded that Au/(Me x O y )/TiO 2 systems were highly effective in the oxidation of 1-phenylethanol, and the catalysts modified with lanthana were the most active, as in the oxidation of 1-octanol. Furthermore, comparing our results on 1-phenylethanol oxidation with other gold supported catalysts (Table 1), it could be concluded that the Au/(Me x O y )/TiO 2 systems are one of the most effective in peroxidative selective oxidation of 1-phenylethanol with TBHP, given the high alcohol/gold ratio (R = 5000), and low temperature (T = 80 • C) used without bases and solvents.
In the absence of a solvent and using TBHP as an oxidizing agent, the most active catalysts are those with a high load of gold and the hydrogen treatment was beneficial. In the case of using solvent and oxygen as an oxidizing agent, as in the oxidation of 1-octanol, the catalysts with the lowest gold content after oxidative pretreatment showed the highest activity. The reason of such a difference most likely is the reduction of some part of unstable gold ions to metallic state in a 0.5% Au/La 2 O 3 /TiO 2_ O 2 catalyst by electron transfer under the influence of TBHP (even catalysts in «as prepared state» could provide high activity in 1-phenylethanol oxidation with TBHP after 6 h). Moreover, it was previously shown that there are the same contribution of stable gold ions in these two catalysts (0.5% Au/La 2 O 3 /TiO 2_ O 2 and 4% Au/La 2 O 3 /TiO 2_ H 2 ); furthermore, the difference in catalytic activity of 1-phenylethanol oxidation on these catalysts was not so noticeable.
The most active catalysts had a high concentration of stable monovalent gold ions, and deactivation of catalysts in 1-phenylethanol oxidation is parallel to the reduction of Au + (Au δ+ ) states, that confirms the cationic nature of the active sites. The promoting role of lanthanum additives consists not only in the formation of the most stable Au + (Au δ+ ) species on the surface of La-modified TiO 2 due to their localization at the strong basic Lewis sites, as proved earlier, but also in the presence of the most active type of oxygen, contributing to a more efficient oxidation of alcohols.
Thus, a general conclusion can be drawn that, despite the different nature of the studied alcohols, the nature of the active sites of Au/(Me x O y )/TiO 2 catalysts in both the aerobic and peroxidative oxidation of 1-phenylethanol and 1-octanol is the same and monovalent gold ions are the active sites in these processes. Herein, the concentration, strength and stability of these sites are determined by the gold content, the nature of the support and modifier, and the pretreatment atmosphere.
The adsorption of 1-phenylethanol on Au clusters was simulated using DFT calculations. Among two different ways of alcohol coordination, that with the OH-group is the most advantageous one. In this case, low-coordinated gold atoms are the most active in the activation of alcohol. The binding energy of 1-phenylethanol with low-coordinated cationic gold atoms through the OH-group is significantly higher than that on the neutral cluster. Therefore, based on the quantum chemical calculations, it was concluded that the cationic sites play an important role in 1-phenylethanol adsorption, what confirms our suggestions on the gold cationic nature based on experimental results. The theoretical results were similar for 2-phenylethanol.
Author Contributions: E.P. carried out the catalysts preparation and pretreatments, performed all catalytic tests, interpreted XPS data and wrote the first draft of the paper; E.K. participated on the conceptualization and methodology of most characterization methods, compared 1-octanol and 1-phenylethanol catalytic results and participated on the writing; A.P.C.R. and L.M.D.R.S.M. were responsible for methodology of catalytic tests in 1-phenylethanol oxidation and supervised those experiments; D.G. was responsible for TPD analyses; S.A.C.C. was responsible for the XPS analyses, D.P. and C.J. performed the DFT calculations and respective interpretation; S.A.C.C., A.J.L.P., A.P., V.C.C. and N.B. provided the means for the realization of this work and contributed to the supervision and paper revision. All authors have read and agreed to the published version of the manuscript. Appendix C Table A1. Catalytic results of the most active catalyst in the aerobic oxidation of 1-octanol and contribution of various electronic states of gold in this catalyst, calculated by XPS. Adapted from [39]. As we can see in Table A1, there was also significant a loss of 50% of its initial activity already in the second cycle of 1-octanol oxidation with O2 (Entry 2, Table A1). At the same time, a proportional decrease of monovalent ions in this sample after the first catalytic cycle is also observed. However, on the basis of H2-TPR analysis it was shown that such deactivation is related to the reduction of unstable monovalent gold ions (20-23%). Moreover, in order to check the stability of the mentioned gold ions and also confirm that they are the active sites, 0.5% Au/La2O3/TiO2_O2 sample was treated in a hydrogen atmosphere at higher temperature (500 °C), for reduction of unstable gold ions. According to the XPS analysis of this sample (Entry 3, Table A1), it was found a decrease of 13% in the surface concentration of Au δ+ ions. This reduced sample was tested in a 1-octanol oxidation reaction. The initial and final conversion of 1-octanol on this sample was reduced almost two times, Figure A2. DRIFT spectra of CO adsorbed on the most active catalysts in peroxidative and aerobic oxidation of 1-phenylethanol. Reproduced from Ref. [43] with permission from the Royal Society of Chemistry, 2019. Table A1. Catalytic results of the most active catalyst in the aerobic oxidation of 1-octanol and contribution of various electronic states of gold in this catalyst, calculated by XPS. Adapted from [39]. As we can see in Table A1, there was also significant a loss of 50% of its initial activity already in the second cycle of 1-octanol oxidation with O 2 (Entry 2, Table A1). At the same time, a proportional decrease of monovalent ions in this sample after the first catalytic cycle is also observed. However, on the basis of H 2 -TPR analysis it was shown that such deactivation is related to the reduction of unstable monovalent gold ions (20-23%). Moreover, in order to check the stability of the mentioned gold ions and also confirm that they are the active sites, 0.5% Au/La 2 O 3 /TiO 2 _O 2 sample was treated in a hydrogen atmosphere at higher temperature (500 • C), for reduction of unstable gold ions. According to the XPS analysis of this sample (Entry 3, Table A1), it was found a decrease of 13% in the surface concentration of Au δ+ ions. This reduced sample was tested in a 1-octanol oxidation reaction. The initial and final conversion of 1-octanol on this sample was reduced almost two times, as the activity of the sample after the recycling test, where it was also observed 13% of gold monovalent ions.