Journal of Functional Biomaterials

16 pages, 1791 KB

Open AccessArticle

Modulating the Optical Properties of Initial Caries Lesions Through Low-Viscosity Resin Infiltration: A Spectrophotometric Evaluation

by Paweł Maksymiuk, Maja Ptasiewicz, Joanna Zubrzycka, Ilona Wójcik-Chęcińska, Katarzyna Sarna-Boś, Piotr Stachurski and Renata Chałas

J. Funct. Biomater. 2026, 17(8), 423; https://doi.org/10.3390/jfb17080423 - 21 Aug 2026

Viewed by 557

Abstract

Objectives: Initial caries lesions (ICLs) are the earliest manifestation of dental caries, characterized by subsurface porosity that affects enamel’s optical properties while maintaining its surface integrity. Resin infiltration is a minimally invasive approach to managing these lesions by penetrating and sealing pores with [...] Read more.

Objectives: Initial caries lesions (ICLs) are the earliest manifestation of dental caries, characterized by subsurface porosity that affects enamel’s optical properties while maintaining its surface integrity. Resin infiltration is a minimally invasive approach to managing these lesions by penetrating and sealing pores with low-viscosity resin, potentially modifying their visual appearance. In order to provide a quantifiable, objective assessment of this optical behavior, this study aimed to evaluate the modulation of color coordinates and VITA Classical shade transitions within the initial lesions immediately following infiltration with Icon Smooth Surface (DMG, Hamburg, Germany). Materials and Methods: Forty-nine extracted human premolars with naturally occurring proximal ICLs (subsurface demineralization) were selected. Lesions were assessed for fluorescence level using 655 nm laser fluorescence (DIAGNOdent pen). Reflectance spectrophotometry (SpectroShade Micro) was employed to record color coordinates in the CIELab and CIELCh spaces, alongside VITA Classical shades, before and after resin infiltration. Results: The infiltration procedure resulted in a statistically significant increase in b* (yellowness) and subsequent C* (chroma) parameters (p < 0.05). VITA Classical shade guide analysis showed an insignificant shift in color distribution according to value (p > 0.05). Conclusions: The present exploratory study provides quantitative insights into the immediate color-modifying effects of a resin infiltration procedure on natural initial proximal caries lesions, complementing the existing literature with objective CIELab/CIELCh and VITA Classical measurements. The p-values should be interpreted as descriptive indicators of potential trends rather than definitive statistical proof. Further research with larger cohorts and long-term follow-up is needed to validate the durability and clinical relevance of these esthetic outcomes. Full article

(This article belongs to the Special Issue Advances in Biomaterials for Oral Health: From Dental Restoration to Regenerative Therapies)

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22 pages, 8914 KB

Open AccessReview

Polyphosphate in Bone Tissue Engineering: From Molecular Mechanisms to Material Design

by Zhangling Nie, Bingqiang Lu, Valentina K. Krut’ko, Anatoly I. Kulak and Feng Chen

J. Funct. Biomater. 2026, 17(8), 422; https://doi.org/10.3390/jfb17080422 - 21 Aug 2026

Viewed by 639

Abstract

Polyphosphate (PolyP) is an inorganic polymer composed of orthophosphate units linked by high-energy phosphate anhydride bonds, widely found in various organisms from bacteria to mammals. In recent years, PolyP has attracted widespread attention in the field of bone tissue engineering due to its [...] Read more.

Polyphosphate (PolyP) is an inorganic polymer composed of orthophosphate units linked by high-energy phosphate anhydride bonds, widely found in various organisms from bacteria to mammals. In recent years, PolyP has attracted widespread attention in the field of bone tissue engineering due to its unique biological characteristics, possessing both osteoinductive activity and metabolic energy supply functions. This article systematically reviews the molecular structure, physicochemical properties, and multiple mechanisms by which PolyP promotes osteogenic differentiation, as well as biomaterial design strategies based on PolyP. PolyP can synergistically promote osteogenic differentiation through multiple mechanisms, including by acting as a phosphate donor, providing metabolic energy, regulating signaling pathways such as Wnt/β-catenin, and modulating the osteoprotegerin/receptor activator of nuclear factor κB ligand (OPG/RANKL) balance. In terms of material design, PolyP can form nano/microparticles with metal ions such as Ca²⁺, Sr²⁺, and Mg²⁺ and can also be compounded with polymers to construct various forms such as hydrogels, bone cement, and three-dimensional (3D)-printed scaffolds. Preclinical studies have shown that PolyP-incorporated materials exhibit excellent osteogenic performance and biocompatibility in bone defect repair, and preliminary clinical studies have also confirmed its feasibility. This article aims to provide a comprehensive overview of the current applications of PolyP-incorporated materials and delineate future directions, challenges, and necessary pathways for their clinical translation. Full article

(This article belongs to the Special Issue Molecular Mechanisms and Biological Procedures of Biomaterials in Medical Applications (2nd Edition))

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13 pages, 12892 KB

Open AccessArticle

In Vitro Cytotoxicity of Three Dimethacrylate-Based Photopolymer Resins for 3D-Printed Dental Restorations: A Qualitative Morphological Screening on Human Fibroblasts

by Teodora-Alina Tincea, Alexia-Ecaterina Cârstea, Vlad-Gabriel Vasilescu, Andreia Cucuruz, Adela Banciu, Adrian-Ionuț Nicoară, Claudia-Gabriela Mateiaș, Ana-Maria Cristina Țâncu, Silviu-Mirel Pițuru, Marina Imre and Lucian-Toma Ciocan

J. Funct. Biomater. 2026, 17(8), 421; https://doi.org/10.3390/jfb17080421 - 20 Aug 2026

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Abstract

The rapid adoption of vat photopolymerization (3D printing) in restorative and prosthetic dentistry has outpaced the independent biological characterization of the dimethacrylate resins on which it relies, even though incompletely converted networks can release residual monomers that are cytotoxic to the adjacent oral [...] Read more.

The rapid adoption of vat photopolymerization (3D printing) in restorative and prosthetic dentistry has outpaced the independent biological characterization of the dimethacrylate resins on which it relies, even though incompletely converted networks can release residual monomers that are cytotoxic to the adjacent oral tissues. This study reports a qualitative in vitro cytotoxicity screening of three commercial photopolymer resins optimized for additive manufacturing: a general-purpose resin (ANYCUBIC) and two resins with a declared dental indication (TEMP PRINT and V-PRINT) performed according to the morphological evaluation described in ISO 10993-5. Aqueous extracts were prepared following ISO 10993-12 and applied to normal human fibroblasts (BJ line, ATCC CRL-2522) at 100% and 50% concentration for 24 h; morphological reactivity was graded on the standardized 0–4 scale by inverted light microscopy against an unexposed control, and supported by a semi-quantitative visual estimate of the proportion of affected cells. All three materials produced a concentration-dependent response. TEMP PRINT retained the best cellular compatibility (grade 1 at 50%, grade 2 at 100%), V-PRINT was intermediate, and ANYCUBIC showed the most pronounced reactivity (approaching grade 3 at 100%), giving an estimated cytotoxicity order of ANYCUBIC > V-PRINT > TEMP PRINT. As a qualitative morphological screening, the findings indicate that the dental-grade resins elicited a milder fibroblast response than the general-purpose photopolymer and support the recommendation that general-purpose resins should not be substituted for certified materials in intraoral use. Confirmation by quantitative viability assays with independent replicates is required before firm conclusions are drawn. In line with the qualitative nature of ISO 10993-5 morphological screening, the reported proportions of morphologically affected cells represent visual scoring rather than direct measurements of cell viability, and the study is therefore presented as an initial biological screening. Full article

(This article belongs to the Special Issue Advanced Dental Biomaterials for Prosthodontic Applications: Functional and Biological Perspectives)

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22 pages, 17873 KB

Open AccessArticle

Inkjet Printing of Drugs into Surface-Embedded Micro-Reservoirs for Drug-Releasing Implants: Influence of Solvent Properties on Deposition Behavior

by Robert Mau, Georg Schnell, Paul Oldorf and Hermann Seitz

J. Funct. Biomater. 2026, 17(8), 420; https://doi.org/10.3390/jfb17080420 - 20 Aug 2026

Viewed by 626

Abstract

Background: Micro-reservoirs in implant surfaces represent a promising drug carrier concept for drug delivery systems. For drug loading, inkjet printing enables highly precise droplet positioning. However, droplet drying influences drug crystallization from printed drug solution. This study investigates how evaporation-driven phenomena affect the [...] Read more.

Background: Micro-reservoirs in implant surfaces represent a promising drug carrier concept for drug delivery systems. For drug loading, inkjet printing enables highly precise droplet positioning. However, droplet drying influences drug crystallization from printed drug solution. This study investigates how evaporation-driven phenomena affect the precision and homogeneity of inkjet-based deposition of a crystallizing drug into exemplary micro-reservoirs. The aim is to guide the selection of suitable solvents and inkjet process parameters. Methods: Laser-drilled micro-reservoirs were fabricated as blind holes with entrance diameters of 100 µm and 400 µm in the surface of specimens of EN 1.4404 (equivalent to AISI 316L) stainless steel, a commonly used biomaterial. The reservoirs were loaded with two different drug solutions using piezoelectric drop-on-demand inkjet printing. Acetylsalicylic acid (ASA) was applied as a model drug representing crystallizing small-molecule drugs. Solvents with markedly different evaporation rates, ethanol (EtOH) as a representative high-volatility solvent and dimethyl sulfoxide (DMSO) as a representative low-volatility solvent, were selected. The number of jetted droplets per dispensing step was varied. Precision and homogeneity of the drug deposition were investigated using light and laser scanning microscopy. Results: Over the course of droplet drying, two phenomena, the coffee-ring effect and creeping, can impair drug deposition quality. The coffee-ring effect leads to inhomogeneous, ring-shaped drug deposits. Creeping is the evaporation-driven spreading of crystalline structures and reduces the precision of drug deposition. The EtOH-based ASA solution (c = 10 g/L) was intensely affected by both phenomena. Inhomogeneities could be partially compensated via tailoring the droplet count per dispensing step. The DMSO-based solution (c = 100 g/L) exhibited a more compact crystallization of ASA (requiring ~20% less volume in an exemplary experiment), no coffee-ring effect, and only minor creeping. Conclusions: The DMSO-based ASA solution enabled a more precise and homogeneous drug deposition than the EtOH-based solution under the investigated printing and crystallization conditions. EtOH-related limitations could be counteracted by controlling the number of jetted droplets per dispensing step. Full article

(This article belongs to the Special Issue Drug- and Ion-Releasing Implants)

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24 pages, 6291 KB

Open AccessArticle

A Computational Framework for the Design and Mechanical Assessment of Biodegradable Airway Stents: Interaction with Rabbit Tracheal Tissue and Preliminary In Vivo Observations

by Ada Ayechu-Abendaño, Letizia Cella, Carmen Sánchez-González, Carmen Sánchez-Matás, José Luis López-Villalobos, Cristina Díaz-Jiménez, Rocío Fernández-Parra and Mauro Malvè

J. Funct. Biomater. 2026, 17(8), 419; https://doi.org/10.3390/jfb17080419 - 20 Aug 2026

Viewed by 509

Abstract

Current airway stents, including silicone and metallic devices, remain associated with important complications such as migration, restenosis, mucus retention and the need for repeated interventions. Biodegradable stents offer a promising alternative by providing temporary mechanical support while avoiding the long-term presence of a [...] Read more.

Current airway stents, including silicone and metallic devices, remain associated with important complications such as migration, restenosis, mucus retention and the need for repeated interventions. Biodegradable stents offer a promising alternative by providing temporary mechanical support while avoiding the long-term presence of a permanent implant. However, the influence of stent geometry and material properties on their mechanical performance and interaction with airway tissue is still not fully understood. This study presents a computational framework integrating computer-aided design and finite element analysis to investigate the mechanical behaviour of biodegradable tracheobronchial stents. Two stent architectures (X-pattern and W-pattern) were analysed over a range of wire thicknesses using two biodegradable materials: a PLA/PCL; 70/30 wt.% blend and AZ31 magnesium alloy. Radial compression, diameter recovery after radial compression and stent–tissue interaction simulations were performed to evaluate the influence of geometry, material selection and design parameters on device performance. The results suggested that both stent geometry and material properties strongly influence the mechanical behaviour of biodegradable airway stents, although they affect different aspects of the stent–tissue interaction. The X-pattern consistently exhibited greater resistance to radial compression, lower elastic diameter recovery after radial compression and improved maintenance of the expanded lumen compared with the W-pattern. Material properties primarily affected the magnitude of the mechanical response, as further confirmed by the quantitative contact-pressure analysis, with AZ31 providing greater radial support, while the spatial distributions of stress and strain within the tracheal wall were mainly governed by the stent architecture. Based on the computational analyses, X-pattern stents manufactured from the PLA/PCL; 70/30 wt.% blend were selected for in vivo evaluation in a rabbit model. Endoscopic observations revealed tissue features that were qualitatively consistent with the mechanical patterns predicted by the numerical simulations, although no direct causal relationship can be established from the available observations. These findings support the ability of the proposed framework to represent the principal aspects of stent–tissue interaction. The proposed computational framework provides a practical tool for the rational design and mechanical assessment of biodegradable airway stents and may facilitate the future development of customised airway prostheses. Full article

(This article belongs to the Section Biomaterials for Tissue Engineering and Regenerative Medicine)

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30 pages, 7306 KB

Open AccessArticle

Comparative Short-Term Electrochemical and Surface Characterization of Biodegradable Mg–Ca–Sr Alloys with Different Strontium Contents

by Gabriela Leață, Dorin Ioan Cocoș, Ramona Feier, Corneliu Munteanu, Fabian Cezar Lupu, Ion Ciucă and Kamel Earar

J. Funct. Biomater. 2026, 17(8), 418; https://doi.org/10.3390/jfb17080418 - 20 Aug 2026

Viewed by 482

Abstract

Biodegradable Mg–Ca–Sr alloys are promising candidates for temporary implant applications, but their degradation behavior is strongly influenced by alloy composition and electrolyte chemistry. This comparative short-term study investigated how increasing the Sr content from 0.5 to 1.5 wt.% influences the electrochemical response and [...] Read more.

Biodegradable Mg–Ca–Sr alloys are promising candidates for temporary implant applications, but their degradation behavior is strongly influenced by alloy composition and electrolyte chemistry. This comparative short-term study investigated how increasing the Sr content from 0.5 to 1.5 wt.% influences the electrochemical response and surface characteristics of cast Mg–0.5Ca–xSr alloys in 0.9% NaCl and calcium- and magnesium-free Dulbecco’s phosphate-buffered saline (DPBS). Potentiodynamic polarization, electrochemical impedance spectroscopy, scanning electron microscopy, quantitative image analysis, and energy-dispersive X-ray spectroscopy were used. Increasing the Sr content reduced the corrosion-current density from 0.0110 to 0.0039 mA/cm² in NaCl and from 0.297 to 0.169 mA/cm² in DPBS, while the corresponding calculated corrosion rates decreased from 2.51 to 0.886 mm/year and from 6.66 to 3.79 mm/year, respectively. Replicated EIS measurements of both alloys showed that Mg–0.5Ca–1.5Sr exhibited higher charge-transfer resistance than Mg–0.5Ca–0.5Sr in both NaCl (253 ± 15 versus 184 ± 14 Ω·cm²) and DPBS (853 ± 48 versus 615 ± 42 Ω·cm²). NaCl-exposed surfaces showed more porous and discontinuous deposits and a greater number of visible pit-like defects, whereas DPBS produced comparatively continuous P-containing deposits. Overall, increasing the Sr content to 1.5 wt.% was associated with lower polarization-derived global corrosion kinetics and a more resistive interfacial response, whereas electrolyte composition strongly influenced both interfacial impedance and surface-deposit morphology. These findings represent comparative short-term electrochemical and surface-characterization data obtained at 23 ± 1 °C and should not be interpreted as measures of long-term physiological degradation or in vivo performance. Full article

(This article belongs to the Special Issue Medical Application of Functional Biomaterials (3rd Edition))

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37 pages, 9859 KB

Open AccessReview

Sustainable Valorization of Biogenic Waste for Bone Repair and Regeneration: A Comprehensive Review of Eggshell and Aquatic Biomaterials

by Shazah Waqar, Tamer A. E. Ahmed and Maxwell T. Hincke

J. Funct. Biomater. 2026, 17(8), 417; https://doi.org/10.3390/jfb17080417 - 19 Aug 2026

Viewed by 592

Abstract

Bone loss represents a significant clinical burden that has driven the development of improved orthopedic graft substitutes. Although current grafting options, including autografts, allografts, and xenografts, have demonstrated considerable therapeutic potential, their widespread application is constrained by limitations such as donor scarcity, limited [...] Read more.

Bone loss represents a significant clinical burden that has driven the development of improved orthopedic graft substitutes. Although current grafting options, including autografts, allografts, and xenografts, have demonstrated considerable therapeutic potential, their widespread application is constrained by limitations such as donor scarcity, limited availability, and associated clinical risks. Consequently, biologically derived materials, including avian eggshells and marine bivalve shells, have emerged as promising alternative sources to produce bone precursor materials and next-generation bone graft substitutes. This review summarizes recent advances in avian eggshell- and marine shell-derived calcium carbonate (CaCO₃) materials for bone regeneration and examines their preclinical evaluation in diverse animal models, including critical-size defects in calvarial, femoral, radial and mandibular bone. Relevant studies published over the past ten years were systematically analyzed, focusing on natural calcium carbonate systems derived from avian eggshell and marine shells, including oyster, mussel, clam, scallop, cockle, and sea urchins. A structured literature search was conducted using PubMed, Scopus, and Google Scholar to identify studies published between 2015 and 2025 investigating eggshell- and aquatic-derived biomaterials for bone repair and regeneration. Eligible studies were screened, and data were comparatively analyzed with respect to biomaterial source, scaffold fabrication, physicochemical characteristics, mechanical performance, biocompatibility, osteogenic potential, and the use of preclinical animal studies. Eggshell-derived biomaterials currently show the strongest translational evidence, while aquatic shell-derived biomaterials remain promising but underexplored for bone regeneration. Furthermore, this review critically examines the scientific, manufacturing, and regulatory challenges that must be addressed before clinical implementation. Full article

(This article belongs to the Special Issue Functional Scaffolds for Hard Tissue Engineering and Surgery)

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16 pages, 2040 KB

Open AccessArticle

A Functional TGF-β/Smad Assay for Targeted Profiling of Demineralized Bone Matrix-Derived Allograft Bioactivity

by Adrian Lendvai, Tobias Weichhart, Hans Peter Weitzenböck, Christoph Wiesner, Rita Seeboeck, Narges Zamani, Michael Matzner, Monika Pichler, Bettina Steiner, Andrea De Luna, Stefan Nehrer and Harald Hundsberger

J. Funct. Biomater. 2026, 17(8), 416; https://doi.org/10.3390/jfb17080416 - 19 Aug 2026

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Abstract

Demineralized bone matrix (DBM)-derived allografts retain extracellular matrix (ECM)-associated factors involved in bone repair, but biochemical protein recovery alone may not predict functional pathway activation. We evaluated the HEK-Blue™ TGF-β Reporter Assay as a targeted method for assessing Smad-dependent reporter activity in DBM-derived [...] Read more.

Demineralized bone matrix (DBM)-derived allografts retain extracellular matrix (ECM)-associated factors involved in bone repair, but biochemical protein recovery alone may not predict functional pathway activation. We evaluated the HEK-Blue™ TGF-β Reporter Assay as a targeted method for assessing Smad-dependent reporter activity in DBM-derived materials. ECM proteins were extracted from cortical demineralized bone granules (DBG) using guanidine hydrochloride (GuHCl) or urea and quantified after extraction, dialysis, and sterile filtration. Reporter cells were stimulated with extracted ECM proteins or directly with processed cortical and cancellous products, including DBG, wet heat-treated DBG formulated as Putty (PHT), and gamma-irradiated PHT (PGI). Non-pooled DBM sponge samples were also tested. Secreted embryonic alkaline phosphatase (SEAP) activity served as the functional reporter readout. Material-only no-cell controls assessed material-derived background. PrestoBlue™ readouts served as exploratory quality controls. The urea-derived extract yielded a higher apparent BCA-detectable protein concentration than the GuHCl-derived extract. Only the GuHCl-derived extract induced increasing SEAP activity at matched protein input. Direct stimulation showed reporter activation above the TNF-α pathway-negative cytokine control for cortical DBG, PHT, PGI, and cancellous DBG. DBM sponges also induced detectable SEAP activity. These findings support targeted functional screening of Smad-dependent TGF-β reporter activation in DBM-derived materials, but not total osteoinductivity assessment. Full article

(This article belongs to the Section Bone Biomaterials)

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34 pages, 1884 KB

Open AccessReview

Applications of DNA Hydrogels in Osteoporotic Bone Defects

by Jiaqi Chen, Huiyu Jia, Xinyue Zhang, Da Liu, Xushuang Jia, Xintong Gu, Hongjuan Wen and Ye Jin

J. Funct. Biomater. 2026, 17(8), 415; https://doi.org/10.3390/jfb17080415 - 18 Aug 2026

Viewed by 663

Abstract

DNA hydrogels are an emerging class of biomaterials with programmability, biodegradability, biocompatibility, and dynamic responsiveness, enabling precise regulation of osteoblast and mesenchymal stem cell (MSC) proliferation and differentiation, activation of key signaling pathways, and promotion of angiogenesis and bone matrix mineralization. In contrast, [...] Read more.

DNA hydrogels are an emerging class of biomaterials with programmability, biodegradability, biocompatibility, and dynamic responsiveness, enabling precise regulation of osteoblast and mesenchymal stem cell (MSC) proliferation and differentiation, activation of key signaling pathways, and promotion of angiogenesis and bone matrix mineralization. In contrast, conventional bone repair materials exhibit limitations including poor mechanical strength, uncontrollable degradation, and inadequate matching with native bone properties, restricting their application in osteoporotic defect repair. Current osteoporotic defect therapies, mainly anti-resorptive and anabolic agents, remain insufficient for many patients. Here, we propose pure and hybrid DNA hydrogels as novel therapeutic platforms to restore the dynamic balance between bone resorption and formation, thereby enhancing osteogenesis and facilitating bone regeneration and remodeling under osteoporotic conditions. Although challenges such as high production cost and long-term safety persist, integration with advanced technologies (e.g., 3D printing and gene editing) may provide theoretical support for further investigation of personalized and intelligent therapeutic strategies at the pre-clinical research stage, offering new insights into osteoporotic bone defects and bone tissue regeneration. Full article

(This article belongs to the Section Bone Biomaterials)

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15 pages, 992 KB

Open AccessArticle

Effects of Intraoperative Ozone Application on Early Implant Stability: A Randomized Split-Mouth Clinical Trial

by Zeliha Başak Çakır Erdil, Hüseyin Akıllı, Şahin Altuğ and Metin Çalışır

J. Funct. Biomater. 2026, 17(8), 414; https://doi.org/10.3390/jfb17080414 - 18 Aug 2026

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Abstract

Background/Objectives: The aim of this study was to evaluate the effect of intraoperative gaseous ozone applied to the implant osteotomy immediately before implant placement on early implant stability using a split-mouth design and resonance frequency analysis (RFA). Methods: This prospective, randomized controlled split-mouth [...] Read more.

Background/Objectives: The aim of this study was to evaluate the effect of intraoperative gaseous ozone applied to the implant osteotomy immediately before implant placement on early implant stability using a split-mouth design and resonance frequency analysis (RFA). Methods: This prospective, randomized controlled split-mouth study included 40 patients receiving 106 implants in bilaterally symmetrical edentulous sites. Patients and outcome assessors were blinded. In the ozone group, ozone gas was applied to the implant site for 60 s immediately before implant placement; the control group underwent the same surgical protocol without ozone application. The primary outcome was the change in implant stability quotient from baseline to three months (ΔISQ). Secondary outcomes included baseline and three-month ISQ values, insertion torque, and postoperative pain assessed using a visual analog scale. Results: All 106 implants were analyzed. No significant between-group differences were observed in baseline ISQ (β = −0.49; 95% CI: −2.73 to 1.75; p = 0.668) or insertion torque (β = −0.62 Ncm; 95% CI: −1.96 to 0.72; p = 0.362). At three months, ISQ was higher in the ozone group (β = 1.91; 95% CI: 0.75 to 3.06; p = 0.001). ΔISQ was also significantly greater in the ozone group (β = 2.40; 95% CI: 0.34 to 4.45; p = 0.022; Cohen’s d = 0.42). Implant stability increased significantly in both groups (both p < 0.001). Postoperative pain did not differ between groups (β = −0.23; 95% CI: −0.83 to 0.36; p = 0.446). Although the effect direction was consistent across sensitivity analyses, ΔISQ was not statistically significant in the patient-level paired analysis (p = 0.089). No adverse events were reported. Conclusions: Intraoperative ozone application was associated with a modest increase in early implant stability without a significant difference in postoperative pain. However, given the small effect size and sensitivity of the findings to the analytical method, further large-scale, multicenter randomized controlled trials are required. Clinically, intraoperative ozone may be considered a potential adjunct to standard implant placement protocols, but the current evidence is insufficient to support its routine use. Full article

(This article belongs to the Section Dental Biomaterials)

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27 pages, 3807 KB

Open AccessArticle

Multidimensional Characterization of Surface Properties and Microbial Biofilm Formation in Commercial Flowable Resin-Based Dental Composites

by Lucian Floare, Otilia Cornelia Bolos, Ramona Dumitrescu, Marioara Nicoleta Caraba, Iasmina-Mădălina Petculescu, Carmen Opris, Octavia Balean, Vanessa Bolchis, Bianca Ioana Todor, Cristina Elena Savencu, Atena Galuscan and Daniela Jumanca

J. Funct. Biomater. 2026, 17(8), 413; https://doi.org/10.3390/jfb17080413 - 18 Aug 2026

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Abstract

The interaction between restorative materials and microbial biofilms plays an important role in restoration longevity and the development of secondary caries. This study aimed to perform a multidimensional characterization of four commercially available flowable resin-based dental composites by evaluating their surface roughness, Vickers [...] Read more.

The interaction between restorative materials and microbial biofilms plays an important role in restoration longevity and the development of secondary caries. This study aimed to perform a multidimensional characterization of four commercially available flowable resin-based dental composites by evaluating their surface roughness, Vickers microhardness, surface morphology, and microbial biofilm formation under standardized in vitro conditions. Filtek™ Bulk Fill Flowable Restorative (3M), Tetric EvoFlow (Ivoclar Vivadent), BRILLIANT Flow (Coltene), and G-ænial™ Universal Injectable (GC) were investigated. Surface roughness was determined by contact profilometry, microhardness was assessed using the Vickers method, and microstructural characteristics were analyzed by scanning electron microscopy (SEM). Microbial biofilm biomass formed by Gram-positive bacteria, Gram-negative bacteria, and Candida albicans, including both reference strains and clinical isolates, was quantified using a crystal violet assay to compare material-dependent microbial colonization. G-ænial™ Universal Injectable exhibited the lowest roughness values, whereas BRILLIANT Flow showed the highest microhardness. SEM analysis revealed a more homogeneous surface morphology for Filtek™ Bulk Fill Flowable Restorative and G-ænial™ Universal Injectable, while Tetric EvoFlow and BRILLIANT Flow displayed increased topographical heterogeneity. Although modest differences in biofilm biomass accumulation were observed among the investigated composites (4.37–12.21% relative biomass reduction compared with the control), no material demonstrated a distinct advantage under the experimental conditions. The integrated evaluation of surface roughness, microhardness, surface morphology, and microbial biofilm formation provides a comparative characterization of commercially available flowable resin-based composites and contributes to a better understanding of material–biofilm interactions. These findings may support the future development of multifunctional restorative biomaterials with improved biofilm-modulating properties. Full article

(This article belongs to the Section Dental Biomaterials)

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3 pages, 157 KB

Open AccessEditorial

State-of-the-Art Dental Adhesives and Restorative Composites

by Jirun Sun

J. Funct. Biomater. 2026, 17(8), 412; https://doi.org/10.3390/jfb17080412 - 18 Aug 2026

Viewed by 388

Abstract

Since the pioneering work of Dr [...] Full article

(This article belongs to the Special Issue State-of-the-Art Dental Adhesives and Restorative Composites)

19 pages, 305 KB

Open AccessArticle

Core Buildup Composite Resins Bond Strength to Dentin and Microhardness Using Universal Adhesives

by Iana Schmitt, Jorge Perdigão and Guilherme Carpena Lopes

J. Funct. Biomater. 2026, 17(8), 411; https://doi.org/10.3390/jfb17080411 - 18 Aug 2026

Viewed by 434

Abstract

The peer-reviewed literature on the use of universal adhesives (UAs) with core build-up composite resins (CBCRs) is limited. UAs may enhance the polymerization of dual-cured composite resins, leading to increased microhardness due to the “touch-cure” effect. This study compared the dentin bond strengths [...] Read more.

The peer-reviewed literature on the use of universal adhesives (UAs) with core build-up composite resins (CBCRs) is limited. UAs may enhance the polymerization of dual-cured composite resins, leading to increased microhardness due to the “touch-cure” effect. This study compared the dentin bond strengths and microhardness of CBCRs bonded with UAs. A total of 480 bovine incisors were randomly allocated into 20 groups (n = 24). Two dual-cured CBCRs, ParaCore (PrCore) and Gradia Core (GrCore), one light-cured CBCR, Clearfil Photo Core (PhotoCore), and Filtek Z250 (Z250) were evaluated. For the dual-cured CBCRs, the UAs from the respective manufacturers were mixed with a dual-cure activator (Act): One Coat 7 Universal (OC7+Act) and G-Premio Bond (GrPB+Act). Three additional UAs were evaluated: Tokuyama Universal Bond II (TUB), Scotchbond Universal Plus Adhesive (SBUP), and Scotchbond Universal Adhesive with Dual Cure Activator (SBU+Act). Shear bond strength was evaluated after 24 h. Additional specimens were prepared for Vickers microhardness (VHN) evaluation, with or without UA application to the bottom surface. Bond strengths ranged from 7.4 ± 4.0 to 37.0 ± 7.5 MPa. GrCore and PrCore showed higher bond strengths with SBU+Act than with their manufacturer-recommended adhesives. Dual-cured CBCRs exhibited lower dentin bond strengths and lower VHN than the light-cured composite resins, and the touch-cure effect was more effective for GrCore and PhotoCore. Full article

(This article belongs to the Special Issue Biomaterials in Restorative Dentistry and Endodontics (2nd Edition))

12 pages, 674 KB

Open AccessArticle

Effect of n-Butyl Cyanoacrylate Coating on the Microhardness of MM-MTA After Exposure to Moisture and Human Blood

by Jurica Matijević, Anja Baraba, Adna Vlahovljak Ferušić, Matea Vidov, Zoran Karlović and Ana Ivanišević

J. Funct. Biomater. 2026, 17(8), 410; https://doi.org/10.3390/jfb17080410 - 17 Aug 2026

Viewed by 459

Abstract

The aim of this study was to evaluate the influence of n-butyl cyanoacrylate (NBCA) coating of freshly mixed mineral trioxide aggregate (MTA) on its microhardness after setting under conditions of moisture and human blood exposure. MicroMega MM-MTA samples were prepared using Teflon molds [...] Read more.

The aim of this study was to evaluate the influence of n-butyl cyanoacrylate (NBCA) coating of freshly mixed mineral trioxide aggregate (MTA) on its microhardness after setting under conditions of moisture and human blood exposure. MicroMega MM-MTA samples were prepared using Teflon molds (6 mm × 4 mm). Four experimental groups (n = 4) were established: control (MTA + phosphate-buffered saline (PBS)), MTA coated with NBCA (PeriAcryl) + PBS, MTA + blood + PBS, and MTA coated with NBCA + blood + PBS. Samples assigned to the blood-exposure groups were exposed to human blood for 15 min, rinsed, and subsequently stored in PBS. Prior to microhardness testing, all samples were incubated in PBS for seven days. Microhardness was measured using a Vickers microhardness tester, with five indentations performed on each specimen (20 measurements per group). Data were analyzed using the Shapiro–Wilk test for normality, Levene’s test, and two-way ANOVA, followed by Tukey’s post hoc test. The analysis revealed significant differences among the experimental groups (p < 0.001). Post hoc analysis demonstrated statistically significant differences between tissue glue-coated and uncoated samples (p < 0.05). Within the limitations of this in vitro study, coating MTA with NBCA tissue glue during the setting period significantly increased its microhardness following exposure to human blood and PBS. Full article

(This article belongs to the Special Issue Property, Evaluation and Development of Dentin Materials)

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17 pages, 1975 KB

Open AccessArticle

Electrosprayed Chitosan–Calcium Phosphate Microshell Composite Coatings on Silanized Titanium Substrates

by Andrew Blass Watson, Matthew J. Atwill, Tomoko Fujiwara, Ranganathan Gopalakrishnan, Jessica Amber Jennings and Joel D. Bumgardner

J. Funct. Biomater. 2026, 17(8), 409; https://doi.org/10.3390/jfb17080409 - 17 Aug 2026

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Abstract

Chitosan and calcium phosphate (CaP) are attractive bioactive coating materials for titanium (Ti) orthopedic implants, but coating approaches must provide uniform deposition, adequate adhesion, and cytocompatibility. This proof-of-concept study evaluated whether CaP microshells could be incorporated into electrosprayed chitosan coatings bonded to silanized [...] Read more.

Chitosan and calcium phosphate (CaP) are attractive bioactive coating materials for titanium (Ti) orthopedic implants, but coating approaches must provide uniform deposition, adequate adhesion, and cytocompatibility. This proof-of-concept study evaluated whether CaP microshells could be incorporated into electrosprayed chitosan coatings bonded to silanized Ti substrates without compromising coating properties. CaP microshells were synthesized using carbon microsphere templates and added to chitosan electrospray solutions at 0.25, 0.5, and 1.0 wt% relative to chitosan. Electrospray parameters were adjusted, and coatings were characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, tensile adhesion testing, water contact angle measurements, and W-20-17 bone marrow stromal cell culture. Increasing capillary diameter and reducing pressure enabled stable deposition of uniform composite coatings containing up to 1.0 wt% CaP. CaP microshells were distributed across the coating surfaces and throughout the coating thickness. Silanization significantly increased coating adhesion compared with non-silanized Ti, while CaP incorporation up to 1.0 wt% did not significantly reduce bond strength. All coatings were hydrophilic and supported viable cell attachment and growth over five days. These findings support the feasibility of electrosprayed chitosan–CaP microshell coatings as adhesive, cytocompatible bioactive coating platforms for Ti implant materials. Full article

(This article belongs to the Special Issue Drug- and Ion-Releasing Implants)

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15 pages, 5834 KB

Open AccessArticle

Evaluation of Polytetrafluoroethylene (PTFE) Tape for Gingival Displacement Using CAD/CAM Analysis and Scanning Electron Microscopy (SEM)

by Laura Monica Rusu, Antonio Pop, Stanca Cuc, Mircea Aurelian Rusu, Sorina Sava, Anca Labunet, Cristina Iosif and Adriana Objelean

J. Funct. Biomater. 2026, 17(8), 408; https://doi.org/10.3390/jfb17080408 - 17 Aug 2026

Viewed by 537

Abstract

Background: This in vitro study compared the effectiveness of polytetrafluoroethylene (PTFE) tape and a conventional cotton retraction cord for gingival displacement and evaluated the mechanical and microstructural characteristics of PTFE tape. Methods: Eight standardized prepared teeth with artificial gingiva were evaluated under three [...] Read more.

Background: This in vitro study compared the effectiveness of polytetrafluoroethylene (PTFE) tape and a conventional cotton retraction cord for gingival displacement and evaluated the mechanical and microstructural characteristics of PTFE tape. Methods: Eight standardized prepared teeth with artificial gingiva were evaluated under three conditions: control, cotton retraction cord, and PTFE tape. After each condition, impressions were digitized using a CAD/CAM system. Sulcular width was measured at four predefined locations and averaged for statistical analysis. PTFE tape was also evaluated by tensile testing before and after sterilization and by scanning electron microscopy (SEM). Results: Mean sulcular widths were 0.716 ± 0.118 mm (control), 0.851 ± 0.140 mm (cotton cord), and 0.907 ± 0.099 mm (PTFE). Repeated-measures ANOVA showed a significant effect of the displacement method (p < 0.001), with PTFE producing significantly greater sulcular enlargement than cotton cord. Qualitative SEM analysis revealed a more homogeneous impression surface morphology following PTFE removal. Sterilization did not significantly affect the tensile properties or microstructural integrity of PTFE tape. Conclusions: Under standardized in vitro conditions, PTFE tape produced the highest gingival sulcular enlargement and maintained favorable mechanical and microstructural properties, supporting its potential as an alternative gingival displacement material. Further clinical studies are required to confirm these findings. Full article

(This article belongs to the Section Dental Biomaterials)

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40 pages, 49805 KB

Open AccessReview

Confined Catalysis of Enzymes: Enhancement Strategies, Materials, and Biosensor Applications

by Lijiao Zhang, Junyi Lu, Chenjie Yan, Wenjing Wang and Dajing Chen

J. Funct. Biomater. 2026, 17(8), 407; https://doi.org/10.3390/jfb17080407 - 15 Aug 2026

Viewed by 683

Abstract

Confined catalysis enhances enzyme activity, stability, and selectivity by encapsulating enzymes in well-defined micro- or nanoscale environments. Unlike conventional immobilization strategies, which primarily stabilize enzymes, this approach actively regulates enzyme behavior, substrate transport, and product removal, addressing challenges in real-world biosensing. In this [...] Read more.

Confined catalysis enhances enzyme activity, stability, and selectivity by encapsulating enzymes in well-defined micro- or nanoscale environments. Unlike conventional immobilization strategies, which primarily stabilize enzymes, this approach actively regulates enzyme behavior, substrate transport, and product removal, addressing challenges in real-world biosensing. In this work, we connect confined catalysis principles with biosensor design requirements, emphasizing tailored microenvironments, hierarchical pore architectures, and cascade reaction optimization to improve sensitivity, selectivity, and operational stability. The review also highlights advanced strategies such as molecular imprinting, aptamer recognition, and DNA scaffold-guided co-localization, which enable precise substrate discrimination and efficient signal amplification. By integrating these confinement strategies with emerging materials, including biological macromolecular materials, amorphous porous materials, and crystalline framework materials, this work outlines pathways to next-generation biosensors with enhanced performance and practical applicability, addressing limitations overlooked in previous studies. Full article

(This article belongs to the Section Biomaterials and Devices for Healthcare Applications)

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21 pages, 6064 KB

Open AccessArticle

Influence of Mastication on Orthodontic Wire Sliding in Nickel–Titanium Brackets with Superelastic Wings

by Jasmin Wichelhaus, Uwe Baumert, Corinna Seidel and Hisham Sabbagh

J. Funct. Biomater. 2026, 17(8), 406; https://doi.org/10.3390/jfb17080406 - 15 Aug 2026

Viewed by 454

Abstract

(1) Background: Orthodontic tooth movement (OTM) during sliding mechanics is influenced by a multitude of interactions. Studies suggest that vibration induced by mastication or usage of electric tooth brushes might impact resistance to sliding (

R_{S}

) in conventional brackets. In superelastic [...] Read more.

(1) Background: Orthodontic tooth movement (OTM) during sliding mechanics is influenced by a multitude of interactions. Studies suggest that vibration induced by mastication or usage of electric tooth brushes might impact resistance to sliding (

R_{S}

) in conventional brackets. In superelastic brackets, archwire sliding might also be altered due to deformation of the bracket wings during mastication. Therefore, the aim of this study was to investigate how the superelastic properties of Nickel–Titanium (NiTi) brackets affect archwire sliding during mastication. (2) Methods: Orthodontic superelastic NiTi archwires with diameters of 0.014” and 0.016” were ligated into four superelastic NiTi brackets. One bracket was positioned with a vertical offset of 1 mm to simulate clinical vertical leveling. A weight force was applied to axially preload the archwire. The occlusal and axial forces affecting the movement of the archwires through the bracket were measured. (3) Results: Low chewing forces caused a measurable axial movement of the 0.014” wire. Increased chewing forces resulted in higher axial movements. The archwire diameter had an impact: 0.014” NiTi required lower masticatory forces to initiate wire motion as compared to the 0.016” NiTi. (4) Conclusions: The superelastic NiTi brackets examined showed that under simulated continuous dynamic masticatory loading, obstacles such as friction or binding could be overcome. Full article

(This article belongs to the Special Issue Functional Dental Materials for Orthodontics and Implants)

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25 pages, 31746 KB

Open AccessArticle

Development of an Electro-Responsive Sorafenib-Loaded Polypyrrole Coating-Modified Nickel–Titanium Alloy for Tumor Ablation Therapy

by Lele Liu, Peng Gu, Dan Xia, Yonghao Wen, Baoe Li, Donghui Wang and Yaohong Wu

J. Funct. Biomater. 2026, 17(8), 405; https://doi.org/10.3390/jfb17080405 - 14 Aug 2026

Viewed by 482

Abstract

Nickel–titanium (NiTi) stents have been widely used for the palliative management of portal vein tumor thrombosis (PVTT) due to their excellent mechanical strength and biocompatibility. However, conventional NiTi implants are therapeutically passive and lack intrinsic antitumor activity, rendering them vulnerable to tumor ingrowth [...] Read more.

Nickel–titanium (NiTi) stents have been widely used for the palliative management of portal vein tumor thrombosis (PVTT) due to their excellent mechanical strength and biocompatibility. However, conventional NiTi implants are therapeutically passive and lack intrinsic antitumor activity, rendering them vulnerable to tumor ingrowth and subsequent restenosis. Electrical stimulation-mediated ablation offers a controllable physical strategy for local tumor clearance; however, the native TiO₂ passivation layer on the NiTi surface restricts its interfacial electroactivity. Electrochemical impedance spectroscopy revealed that the PPy coating significantly reduced the interfacial impedance of the NiTi substrate. After loading with sorafenib, NiTi−PPy−S maintained good electrochemical responsiveness and enabled voltage-dependent drug release characteristics. Under an applied potential of 0.9 V for 24 h, approximately 10.522 ± 0.295 μg/cm² of the loaded sorafenib was released mechanistically, whereas only 0.249 ± 0.171 μg/cm² was released through passive diffusion over the same period. The antitumor effect is closely associated with ES-triggered Ca²⁺ influx, mitochondrial Ca²⁺ overload, loss of mitochondrial membrane potential, and caspase-3-dependent apoptosis. These findings demonstrate that electro-responsive PPy can transform passive NiTi implants into active antitumor therapeutic interfaces, offering a promising strategy for developing multifunctional implants for PVTT treatment. Full article

(This article belongs to the Special Issue Biomaterials for Drug Delivery and Cancer Therapy)

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20 pages, 6749 KB

Open AccessArticle

Finite Element Analysis of Stress Distribution in Healthy and Restored Mandibular Molars with Zirconia and Lithium Disilicate Crowns Under Vertical and Oblique Loading

by Rosa Alicia Hernández-Vázquez, Rodrigo Arturo Marquet-Rivera, Octavio Alejandro Mastache-Miranda, Karina Gabriela Madrigal-Carrillo and Rosa Adriana Rivera-Díaz

J. Funct. Biomater. 2026, 17(8), 404; https://doi.org/10.3390/jfb17080404 - 14 Aug 2026

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Abstract

The mechanical compatibility between dental restorative materials and the natural tooth structure is a relevant factor for long-term clinical performance. Although zirconia (yttria-stabilized tetragonal zirconia polycrystal, Y-TZP) and lithium disilicate are widely used for full-coverage crowns, their biomechanical interaction with the underlying dentin [...] Read more.

The mechanical compatibility between dental restorative materials and the natural tooth structure is a relevant factor for long-term clinical performance. Although zirconia (yttria-stabilized tetragonal zirconia polycrystal, Y-TZP) and lithium disilicate are widely used for full-coverage crowns, their biomechanical interaction with the underlying dentin and pulp under functional loading remains insufficiently characterized. This study reports a comparative finite element analysis (FEA) of a mandibular first molar under vertical (200 N, axial) and oblique (200 N, 30°) loading, evaluating three configurations: an intact healthy tooth, a zirconia Y-TZP full-coverage crown, and a lithium disilicate full-coverage crown. The three-dimensional geometry was obtained from a cone-beam computed tomography (CBCT) study of a caries-free mandibular first molar, previously described and verified by the present group, and was analyzed in ANSYS Workbench (Static Structural). Von Mises equivalent stress, maximum principal stress and total deformation were obtained for enamel or restoration, dentin, and pulp in each configuration. Zirconia produced the highest stress concentrations in the coronal restoration (88.4 MPa vertical; 174.5 MPa oblique), exceeding the healthy enamel baseline by 57.6% and 89.7%, respectively. Both restorative materials reduced dentin stress relative to the healthy tooth, consistent with the stress-shielding effect driven by elastic-modulus mismatch. Under oblique loading, the maximum principal stress in healthy enamel reached 61.7 MPa, approaching or exceeding the upper bound of the reported tensile strength range (~10–40 MPa) and identifying oblique loading as the more demanding of the two conditions analyzed. Within the limitations of the present finite element model, lithium disilicate demonstrated a more favorable stress distribution, with dentin stress values closer to the intact-tooth baseline. The model does not include a luting cement layer, a periodontal ligament, the dentin–enamel junction, anisotropic tissue behavior or cyclic loading, and no experimental validation was performed; the results are therefore presented as a controlled numerical comparison between three configurations under the specific conditions simulated, and not as direct clinical selection criteria. Full article

(This article belongs to the Special Issue Biomechanical Studies and Biomaterials in Dentistry (3rd Edition))

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25 pages, 4547 KB

Open AccessArticle

Engineering Cobalt Bis(dicarbollide)-Loaded Polymeric Nanocarriers: From Controlled Fabrication to Drug Delivery for BNCT

by Maksim A. Klimenko, Maria B. Sokol, Mariia R. Mollaeva, Nikita G. Yabbarov, Ivan A. Gulyaev, Margarita V. Chirkina, Olga E. Kamaeva, Igor B. Sivaev, Anna A. Druzina, Vladimir I. Bregadze, Irina Yu. Nikolaeva, Andrey Yu. Bychkov, Vladimir A. Khvostikov, Zhanna P. Burmii, Sergei I. Obydennyi and Elena D. Nikolskaya

J. Funct. Biomater. 2026, 17(8), 403; https://doi.org/10.3390/jfb17080403 - 14 Aug 2026

Viewed by 617

Abstract

Poly(lactic-co-glycolic) acid nanoparticles (NPs) with the addition of the sodium salt of cobalt bis(1,2-dicarbollide) (COSAN) were developed for boron neutron capture therapy (BNCT). The Box–Behnken design was applied to predict an optimal value of variables and to obtain the desired NP characteristics. The [...] Read more.

Poly(lactic-co-glycolic) acid nanoparticles (NPs) with the addition of the sodium salt of cobalt bis(1,2-dicarbollide) (COSAN) were developed for boron neutron capture therapy (BNCT). The Box–Behnken design was applied to predict an optimal value of variables and to obtain the desired NP characteristics. The optimized NPs had a size of 166 nm and a COSAN loading of 16%. Based on FTIR and UV-VIS spectrophotometry data, it was found that COSAN maintains chemical stability during the encapsulation process and exhibits prolonged release from NPs. A study of in vitro cytotoxicity and accumulation of the NPs on B16 cells revealed their low toxicity and high accumulation in cells after 24 h. In vivo analysis in B16-bearing BALB/c nude mice showed preliminary tumor-associated ¹⁰B distribution 12 h after iv administration of a deliberately low NP-CPPC dose (50 µg per mouse). The estimated intratumoral concentration was 0.31 µg ¹⁰B/g, which is below commonly cited BNCT delivery benchmarks. These results show high potential of the formulation, but require further optimization of dose, timing, biodistribution, normal-tissue exposure, tolerability and therapeutic-dose BNCT evaluation. Full article

(This article belongs to the Special Issue State of the Art in Biomaterials for Drug Delivery (2nd Edition))

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19 pages, 6189 KB

Open AccessTechnical Note

An Autologous Tooth-Shell Technique for Ridge Reconstruction and Immediate Implant Placement: A Two-Case Technical Report and Review of the Literature

by Jan Łoś, Justyna Szałkowska-Łoś, Michał Pikuła and Paulina Adamska

J. Funct. Biomater. 2026, 17(8), 402; https://doi.org/10.3390/jfb17080402 - 14 Aug 2026

Viewed by 404

Abstract

Background: Autologous dentin has emerged as a potential grafting material in regenerative oral surgery because of its structural and biological similarities to bone. This technical report describes a modified tooth-shell technique used for horizontal ridge reconstruction with simultaneous implant placement in two [...] Read more.

Background: Autologous dentin has emerged as a potential grafting material in regenerative oral surgery because of its structural and biological similarities to bone. This technical report describes a modified tooth-shell technique used for horizontal ridge reconstruction with simultaneous implant placement in two clinical cases involving the aesthetic zone. Case Presentation: The first case involved a 29-year-old female patient requiring extraction of a partially impacted, buccally positioned maxillary third molar (tooth 28). The extracted tooth was processed intraoperatively to obtain a dentin shell, which was fixed as a buccal plate during simultaneous implant placement. The remaining defect was filled with sticky bone prepared from biphasic calcium phosphate combined with platelet-rich fibrin. Radiographic evaluations performed at 4 and 6 months demonstrated stable ridge contours, while clinical follow-up continued for 2 years with maintenance of implant function and peri-implant tissue stability. The second case involved a 58-year-old female patient with a previous failure of immediate implantation in the anterior maxilla associated with implant overload, loss of implant stability, and buccal bone deficiency. After soft- and hard-tissue healing, ridge reconstruction was performed using a dentin shell prepared from tooth 18 together with simultaneous implant placement and immediate provisional restoration. Follow-up after 6 months revealed stable peri-implant tissues, a satisfactory aesthetic outcome, and successful prosthetic rehabilitation. Conclusions: The modified tooth-shell technique using autologous dentin provided satisfactory preliminary clinical and radiographic outcomes in both cases. These findings should be considered hypothesis-generating and require confirmation in larger prospective studies with longer follow-up periods. Full article

(This article belongs to the Special Issue Biomaterials in Dentistry: Current Status and Advances)

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49 pages, 1984 KB

Open AccessReview

Hydrogel Implementing Drug Delivery in Cranial Bone Tissue Engineering

by Martina Salvati, Alessia Vita, Alessandro Arcovito, Ornella Parolini, Federica Tiberio and Wanda Lattanzi

J. Funct. Biomater. 2026, 17(8), 401; https://doi.org/10.3390/jfb17080401 - 14 Aug 2026

Viewed by 466

Abstract

Cranial bone defects remain a significant clinical challenge due to their limited intrinsic regenerative capacity and the complexity of coordinating osteogenesis, angiogenesis, and immune responses within a confined and poorly vascularised environment. Conventional approaches, including autologous grafts and synthetic implants, provide structural support [...] Read more.

Cranial bone defects remain a significant clinical challenge due to their limited intrinsic regenerative capacity and the complexity of coordinating osteogenesis, angiogenesis, and immune responses within a confined and poorly vascularised environment. Conventional approaches, including autologous grafts and synthetic implants, provide structural support but fail to actively modulate the biological processes required for effective bone regeneration. In this context, hydrogel-based systems have emerged as versatile platforms for localized and controlled drug delivery in cranial bone tissue engineering (BTE). This review provides a comprehensive overview of hydrogel-based delivery strategies designed to regulate the spatiotemporal presentation of bioactive agents within cranial defects. The main classes of hydrogels, natural, synthetic, semi-synthetic, and hybrid systems, are discussed in relation to their physicochemical properties and suitability for drug delivery applications. Current delivery approaches are analysed, including cell-free systems (growth factors, peptides, bioactive ions, nucleic acids, and small molecules drugs), cell-based platforms, and multifunctional systems integrating secondary carriers such as nanoparticles (NPs), microparticles (MPs), and extracellular vesicles (EVs). Particular emphasis is placed on how hydrogel design parameters, including crosslinking density, degradation kinetics, and responsiveness to microenvironmental cues, govern therapeutic release and influence regenerative outcomes. Emerging strategies and key translational challenges are also highlighted. Full article

(This article belongs to the Special Issue Smart Biomaterials for Precision Medicine: From Targeted Nanodelivery to Gene and Tissue Engineering)

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16 pages, 7313 KB

Open AccessArticle

Bacitracin-Loaded Type I Collagen/Bacterial Cellulose Dressing for the Repair of Infected Wounds

by Chengcheng Gong, Wenwen Jiang, Siyu Huai, Huiling Tong, Nan Tang, Xidong Wu and Haiyong Ao

J. Funct. Biomater. 2026, 17(8), 400; https://doi.org/10.3390/jfb17080400 - 13 Aug 2026

Viewed by 395

Abstract

In this study, bacitracin—a small-molecule antibacterial agent—was incorporated into the network structure of a type I collagen/bacterial cellulose (Col/BC) composite via in situ recombination and impregnation adsorption, leveraging the well-established drug-loading capacity and sustained-release characteristics of type I collagen. The obtained BA@Col/BC (BA@CBC) [...] Read more.

In this study, bacitracin—a small-molecule antibacterial agent—was incorporated into the network structure of a type I collagen/bacterial cellulose (Col/BC) composite via in situ recombination and impregnation adsorption, leveraging the well-established drug-loading capacity and sustained-release characteristics of type I collagen. The obtained BA@Col/BC (BA@CBC) functional dressing features a well-defined nanoporous architecture, high porosity (83.9 ± 1.8%), rapid water absorption kinetics, substantial water absorption capacity (48.7 ± 2.4 g/g), and satisfactory moisture permeability (2984 ± 56 g·m²·day). Critically, the introduction of type I collagen not only effectively delays the release of bacitracin, but also significantly improves the cytocompatibility of the bacterial cellulose-based dressing. BA@CBC exhibits powerful antibacterial activities against S. aureus and MRSA, and can promote the proliferation of NIH3T3 and HUVEC, demonstrating excellent cytocompatibility. In vivo studies in a murine infected-wound model revealed that BA@CBC significantly accelerates wound closure compared to controls; histological evaluation further showed the most organized re-epithelialization, robust granulation tissue formation, and de novo hair follicle regeneration in the BA@CBC group. Fluorescence immunohistochemical analysis confirmed markedly reduced expression of pro-inflammatory cytokines IL-1β and TNF-α in BA@CBC-treated wounds, indicating effective suppression of excessive inflammation and consequent improvement of the wound microenvironment. Collectively, BA@CBC integrates favorable physicochemical properties, sustained antibacterial functionality, and superior cytocompatibility—positioning it as a promising candidate for clinical management of infected wounds. Full article

(This article belongs to the Special Issue Spotlight on Biomedical Coating Materials)

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18 pages, 3723 KB

Open AccessSystematic Review

Association Between Cross-Sectional Geometry and Cyclic Fatigue Resistance of Nickel–Titanium Endodontic Instruments: A Systematic Review

by Mariya Kubatska, Julia Kensy, Joanna Cygankiewicz, Maja Gajewska, Anna Błaszczyk-Pośpiech, Kamil Wesołek, Agata Małyszek, Jacek Matys and Maciej Dobrzyński

J. Funct. Biomater. 2026, 17(8), 399; https://doi.org/10.3390/jfb17080399 - 12 Aug 2026

Viewed by 542

Abstract

This systematic review aimed to evaluate whether cross-sectional geometry is associated with cyclic fatigue resistance of nickel–titanium (NiTi) endodontic instruments and to identify the geometric and materials-related features most frequently associated with improved fatigue performance. The protocol was prospectively registered on the Open [...] Read more.

This systematic review aimed to evaluate whether cross-sectional geometry is associated with cyclic fatigue resistance of nickel–titanium (NiTi) endodontic instruments and to identify the geometric and materials-related features most frequently associated with improved fatigue performance. The protocol was prospectively registered on the Open Science Framework (OSF). PubMed, Scopus, Embase, Web of Science, and WorldCat were searched in April 2026 in accordance with PRISMA 2020. Eligible studies were comparative in vitro investigations that explicitly evaluated cross-sectional geometry or a related geometric parameter as a prespecified study factor and reported quantitative cyclic fatigue outcomes. Of 107 screened records, 66 full-text reports were assessed and 23 studies were included in the qualitative synthesis. Twenty-two studies had a medium risk of bias and one had a low risk of bias according to the Quality Assessment Tool for In Vitro Studies (QUIN). Cyclic fatigue resistance was most often reported as time to fracture or number of cycles to failure. Instruments with reduced metal mass, smaller core volume, lower cross-sectional area, and greater flexibility tended to demonstrate higher fatigue resistance in curved canals. S-shaped and double-S-shaped cross-sections were most consistently associated with favorable outcomes; however, this association is more plausibly related to reduced bending stiffness and canal-wall contact forces than to increased intrinsic material fatigue strength. Flat-side designs did not show a consistent advantage. The evidence was limited by heterogeneous testing protocols and residual confounding by alloy, heat treatment, taper, manufacturing, surface condition, and kinematics. No meta-analysis or quantitative dimensional correlation was feasible because testing conditions and detailed geometric parameters were inconsistently reported. Full article

(This article belongs to the Special Issue Current and Future Development Trends of Materials and Advanced Technologies Used in Endodontics)

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51 pages, 1112 KB

Open AccessReview

Comparative Analysis of Bone Remodeling Dynamics and Bone Vitality Following Standard Bone Augmentation Procedures: A Review

by Nikola Gapińska, Marek Śmielecki and Kornel Krasny

J. Funct. Biomater. 2026, 17(8), 398; https://doi.org/10.3390/jfb17080398 - 12 Aug 2026

Viewed by 414

Abstract

Bone augmentation procedures are widely used in implant dentistry to reconstruct deficient alveolar bone and support implant placement. However, outcomes are often evaluated mainly by defect filling, volumetric stability, or implant survival, which do not necessarily reflect the biological quality of regenerated tissue. [...] Read more.

Bone augmentation procedures are widely used in implant dentistry to reconstruct deficient alveolar bone and support implant placement. However, outcomes are often evaluated mainly by defect filling, volumetric stability, or implant survival, which do not necessarily reflect the biological quality of regenerated tissue. This narrative review synthesizes human and supporting mechanistic evidence across autogenous, allogeneic, xenogeneic, and alloplastic grafts, including biologically functionalized constructs, with emphasis on bone remodeling dynamics and functional regeneration. Available evidence indicates that volumetric preservation alone should not be considered equivalent to complete bone regeneration and that remodeling is material-, product-, processing-, and indication-dependent. Augmented sites may contain newly formed vital bone together with persistent residual graft particles, forming a hybrid tissue whose significance depends on graft integration, vascularization, remodeling capacity, and the intended therapeutic objective. Persistent particles may influence the local immune microenvironment and, under unfavorable conditions, contribute to foreign body reactions or other complications, although such events appear uncommon and causal relationships remain uncertain. Biomaterial assessment should integrate quantitative, structural, and biological parameters, while residual graft persistence should be interpreted in an indication-specific manner rather than as evidence of treatment failure or inferior long-term performance in isolation. Full article

(This article belongs to the Special Issue Dental Biomaterials in Implantology and Orthodontics)

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32 pages, 4702 KB

Open AccessReview

Biomimetic Dermal Substitutes for Skin Reconstruction: Advances in Scaffold Design, Biological Integration, and Clinical Performance

by Bogdan Mircea Măciuceanu Zărnescu, Iulia Ioana Lungu, Adelina-Gabriela Niculescu, Alexandru Scafa Udriște, Constantin Cosmin Baciu, Daniela Anghel, Adrian Radu Rădulescu, Alexandru Mihai Grumezescu and Sebastian Vâlcea

J. Funct. Biomater. 2026, 17(8), 397; https://doi.org/10.3390/jfb17080397 - 12 Aug 2026

Viewed by 502

Abstract

Reconstructive surgery still faces various challenges when dealing with the reconstruction of complex skin defects, regardless of their origin (e.g., trauma, burns). Split-thickness autologous skin grafts remain the gold standard; however, this technique comes with several limitations, including donor site morbidity, infection, and [...] Read more.

Reconstructive surgery still faces various challenges when dealing with the reconstruction of complex skin defects, regardless of their origin (e.g., trauma, burns). Split-thickness autologous skin grafts remain the gold standard; however, this technique comes with several limitations, including donor site morbidity, infection, and scarring. Since extensive research on conventional techniques has not provided sufficient long-term results, there has been a shift toward dermal substitutes made from regenerative biomaterials that can aid wound management and support tissue restoration. This structured review will introduce the challenges posed by complex skin defects, the limitations of autologous skin grafts and conventional reconstruction techniques, and the emerging field of dermal substitutes and biomimetic scaffolds. Moreover, the native dermis is presented as a blueprint for biomimetic scaffold design, along with key principles to consider when engineering a dermal scaffold, from architecture and porosity to angiogenic potential. A critical examination of the main classes of available dermal substitutes and their clinical performance is conducted, highlighting their limitations and the translational gaps in current evidence. Although the current pool of biomaterials still faces challenges in accurately mimicking the properties and architecture of natural skin, future perspectives offer promising alternatives that could overcome these limitations. Full article

(This article belongs to the Special Issue Novel Biomaterials for Tissue Engineering (2nd Edition))

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18 pages, 2697 KB

Open AccessArticle

Dipyridamole-Coated 3D-Printed β-Tricalcium Phosphate Scaffolds: Spectrophotometric Characterization, Drug Release Kinetics, and In Vitro Evaluation to Guide Critical-Sized Bone Defect Repair Studies

by Purva Rasane, Vasudev Vivekanand Nayak, Lahiru Chamara Weerasinghe Arachchige, Eleni Rice, Zeinab Fotouhi Ashin, Bharath Venkatesan, Venu Varanasi, Noriaki Ono, Simon Young and Lukasz Witek

J. Funct. Biomater. 2026, 17(8), 396; https://doi.org/10.3390/jfb17080396 - 11 Aug 2026

Viewed by 548

Abstract

Critical-sized bone defects remain a significant clinical challenge, and dipyridamole (DIPY)-coated 3D-tricalcium phosphate (β-TCP) scaffolds have shown promising osteogenic efficacy in preclinical models. However, the literature on the systematic physicochemical characterization of this scaffold system, including optimization of DIPY loading parameters, release kinetics, [...] Read more.

Critical-sized bone defects remain a significant clinical challenge, and dipyridamole (DIPY)-coated 3D-tricalcium phosphate (β-TCP) scaffolds have shown promising osteogenic efficacy in preclinical models. However, the literature on the systematic physicochemical characterization of this scaffold system, including optimization of DIPY loading parameters, release kinetics, and surface properties, is lacking. This study addresses these gaps by characterizing DIPY-loaded 3D-printed β-TCP scaffolds across solid and porous architectures, three coating concentrations (10, 100, and 1000 µM), and three coating volumes (250, 500, and 1000 µL). Under static PBS conditions, drug release over 21 days was quantifiable only at 1000 µM, and release-kinetics modeling (zero-order, Higuchi, and Korsmeyer–Peppas) was therefore restricted to this highest concentration. At 1000 µM, both scaffold types showed biphasic release profiles, with standard empirical models reasonably approximating the overall kinetics, while not fully capturing the biphasic behavior over the entire duration. Porous scaffolds showed significant volume-dependent release (p = 0.002, η² = 0.88), attributable to drug penetration into the interconnected macropore network, whereas solid scaffolds displayed volume-independent release confined to external surfaces. Scanning electron microscopy revealed concentration-dependent needle-shaped DIPY crystal deposition while contact angle measurements indicated no significant changes in surface hydrophilicity. MTT assay demonstrated biocompatibility at all concentrations, with viability influenced by coating volume rather than drug concentration. These findings establish a foundational physicochemical framework for DIPY-loaded β-TCP scaffolds, providing the baseline data necessary to guide subsequent biological evaluation and translational efforts toward critical-sized craniofacial and orthopedic bone defect repair. Full article

(This article belongs to the Special Issue Engineering Regeneration: Biomaterials, Biology, and Translation)

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18 pages, 2519 KB

Open AccessArticle

Clinical Severity, Pre- and Post-Explantation Cytokine Profile and SEM/EDX-Defined Corrosion-Related Findings in Advanced Peri-Implantitis: Preliminary Results from the PERI-EDU Prospective Exploratory Clinical Study

by Marzena Dominiak, Alicja Baranowska, Jacek Matys, Artur Pitułaj, Barbara Sterczała, Eduard Valmaseda-Castellón, Ricardo Castro Alves, Piero Papi, Umberto Romeo, Wojciech Simka, Artur Maciej, Julia Kensy and Paweł Kubasiewicz-Ross

J. Funct. Biomater. 2026, 17(8), 395; https://doi.org/10.3390/jfb17080395 - 11 Aug 2026

Viewed by 463

Abstract

Background: Peri-implantitis is a chronic condition that reflects an interaction between local tissue destruction, systemic inflammation and corrosion-related implant alterations. This preliminary prospective exploratory clinical study aimed to characterize the systemic cytokine profile of patients with advanced peri-implantitis requiring explantation and to explore [...] Read more.

Background: Peri-implantitis is a chronic condition that reflects an interaction between local tissue destruction, systemic inflammation and corrosion-related implant alterations. This preliminary prospective exploratory clinical study aimed to characterize the systemic cytokine profile of patients with advanced peri-implantitis requiring explantation and to explore associations between clinical severity, cytokine behavior and scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDX)-defined corrosion-related findings. Methods: Thirteen patients with active advanced peri-implantitis were included. Probing depth and cone-beam computed tomography (CBCT)-based bone loss were assessed at four implant surfaces. Peripheral blood was collected before and 4 weeks after explantation. Serum interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), interleukin-12p70 (IL-12p70), tumor necrosis factor alpha (TNF-α), high-sensitivity C-reactive protein (hsCRP), and fibrinogen were analyzed. Explanted implants underwent SEM/EDX assessment and were classified according to the presence or absence of SEM/EDX-defined corrosion-related findings. Results: IL-6 showed the strongest and most consistent associations with clinical and radiographic severity, whereas hsCRP and fibrinogen were not similarly related. Cytokines did not show a uniform post-explantation decrease after explantation. SEM/EDX-defined corrosion-related findings were not associated with higher baseline cytokines, but exploratory subgroup analysis suggested a divergent TNF-α trajectory. Conclusions: These findings suggest that IL-6 may reflect the clinical inflammatory burden of advanced peri-implantitis, while corrosion-related findings may be associated with post-explantation TNF-α behavior. Larger controlled studies are required. Full article

(This article belongs to the Special Issue Biomaterials Applied in Dental Sciences (2nd Edition))

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Open AccessArticle

Antimicrobial Gelatin Methacryloyl (GelMA)/Bioactive Glass Dental Adhesives

by Tianyuan Zhao, Andrew M. Edwards, Adam D. Celiz and Julian R. Jones

J. Funct. Biomater. 2026, 17(8), 394; https://doi.org/10.3390/jfb17080394 - 10 Aug 2026

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Abstract

Oral diseases, such as periodontitis, remain a major global concern due to their high incidence and significant morbidity. The principal drivers are bacterial overgrowth and associated inflammation. Bioactive glass of the 45S5 Bioglass^® composition (BG) is known for its remineralising, osteogenic and [...] Read more.

Oral diseases, such as periodontitis, remain a major global concern due to their high incidence and significant morbidity. The principal drivers are bacterial overgrowth and associated inflammation. Bioactive glass of the 45S5 Bioglass^® composition (BG) is known for its remineralising, osteogenic and antibacterial effects via ion release, but its application has been limited in oral environments. In this study, a photocurable GelMA hydrogel was developed as a carrier matrix for BG (1–15% w/v) to obtain synergy between the adhesive properties of the GelMA and the bioactivity of the glass. Incorporation of 10% w/v BG improved ultimate tensile strength (97 kPa) compared to pure GelMA (58 kPa) and reduced swelling by 18%. The composites showed ~60% higher adhesive strength on collagen sheets than GelMA alone. Tensile bonding strengths reached 54 kPa on collagen sheets and 23 kPa on tooth sections. Lap-shear adhesive strengths were 44 kPa on collagen and 18 kPa on implant metal. In vitro studies confirmed the composite’s biocompatibility with dental pulp stem cells and antibacterial activity against Escherichia coli and methicillin-resistant Staphylococcus aureus. Overall, the GelMA/BG composite presents a multifunctional platform for dental remineralisation with promising mechanical, adhesive and antibacterial performance. Full article

(This article belongs to the Special Issue Advanced Bioglasses, Bioceramics and Their Composites: State of the Art and Applications)

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J. Funct. Biomater., Volume 17, Issue 8 (August 2026) – 71 articles

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