Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico

The resistance of cancer and Helicobacter pylori to several drugs reflects a worldwide problem, and it has been the intention of numerous researchers to overcome this problem. Thus, in this study, Acacia nilotica fruits were subjected to HPLC analysis to detect their phenolic compounds and flavonoids. Moreover, A. nilotica‘s anti-H. pylori activity and its inhibitory activity against human hepatocellular carcinoma (HepG-2 cells) were reported. Various compounds with different concentrations, such as ferulic acid (5451.04 µg/mL), chlorogenic acid (4572.26 µg/mL), quercetin (3733.37 µg/mL), rutin (2393.13 µg/mL), gallic acid (2116.77 µg/mL), cinnamic acid (69.72 µg/mL), hesperetin (121.39 µg/mL) and methyl gallate (140.45 µg/mL), were detected. Strong anti-H. pylori activity at 31 mm was reported, compared to the positive control of the 21.67 mm inhibition zone. Moreover, the MIC and MBC were 7.8 µg/mL and 15.62 µg/mL, respectively, while the MIC and MBC of the positive control were 31.25 µg/mL. The concentration of MBC at 25%, 50% and 75% reflected H. pylori’s anti-biofilm activity of 70.38%, 82.29% and 94.22%, respectively. Good antioxidant properties of the A. nilotica flower extract were documented at 15.63, 62.50, 250 and 1000 µg/mL, causing the DPPH scavenging percentages of 42.3%, 52.6%, 65.5% and 80.6%, respectively, with a IC50 of 36.74 µg/mL. HepG-2 cell proliferation was inhibited (91.26%) using 500 µg/mL of flower extract with an IC50 of 176.15 µg/mL, compared to an IC50 of 395.30 µg/mL used against human normal melanocytes. Molecular docking was applied to investigate ferulic acid with the H. pylori (4HI0) crystal structure to determine the best binding mode that interacted most energetically with the binding sites. Molecular docking indicated that ferulic acid was a proper inhibitor for the 4HI0 protein enzyme of H. pylori. A low energy score (−5.58 Kcal/mol) was recorded as a result of the interaction of ferulic acid with the residue’s SER 139 active site caused by the O 29 atom, which was important for its antibacterial activity.


Introduction
Since the dawn of ancient times, humans have used medicinal plants, and they have continued to play a significant role in the development of safer and more efficient natural drug delivery methods [1][2][3]. The black babul, kikar or the gum arabic tree, often referred to as Acacia nilotica (native to Egypt), is a member of the Fabaceae family, which is extensively distributed throughout several nations. Being a common and significant plant, it is used in a variety of ways from medicine (root, bark, leaves, forbear, gum and pods) to feed (leaves and shoots for animals) and dyeing (leather color) [4]. All parts of A. nilotica, including 180 g/mL and 5 mg/mL, respectively. The stronger anti-H. pylori and significant inhibition of urease activity has been reported via the extracts of A. nilotica [25]. In vitro and in vivo tests of the A. nilotica extract revealed high anti-urease activity and significant anti-adhesion properties [26]. Therefore, these studies indicated that A. nilotica could influence the colonization of H. pylori in the stomach, and its potential pharmaceutical use for the eradication of H. pylori could be further investigated. Moreover, these compounds were chosen for molecular docking studies with targeted H. pylori virulent proteins such as cytotoxic-associated gene A (CagA), vacuolating-associated gene (VacA), urease, and gastric cancer-induced signaling proteins such as the epidermal growth factor receptor and the vascular endothelial growth factor receptor. All the compounds of the A. nilotica leaf extract demonstrated good drug-likeness scores. A. nilotica leaf extract could act as a potent anti-H. pylori and a gastric cancer drug, as demonstrated by the validation of the in vitro and in silico results of Sampath et al. [27].
Different species of Acacia were tested to repress the proliferation of several cancer cells. For instance, kidney, breast and liver cells were repressed by the extract of aerial parts of Acacia hamulosa, A. salicina, A. laeta and A. tortilis [19]. In addition, human cancer cell lines, such as those of MCF-7, A549 and THP-1 cells, were influenced by A. catechu and A. nilotica extracts [28,29]. A. nilotica leaf extracts exhibited anticancer activity against Hep-2 (cervical cancer) and MDA-MB-231 (breast cancer) [30]. Numerous scientific texts have almost wholly focused on the biological screening as well as the phytochemical constituents of the leaves, seeds, pod, root and bark of A. nilotica. However, the biological effects of A. nilotica flowers are limited. In this study, therefore, an investigation has been conducted to test the extract of A. nilotica flowers against H. pylori and its anticancer activities.

Collection and Extraction of Acacia nilotica Flowers
A. nilotica was grown at the canal banks crossing the Delta, Monufia Governorate, Egypt. Flowers of A. nilotica were collected from three plants of the same species that were identified according to the botanical keys [31]. The plant was identified by Prof. Marei A. Hamed, Prof. of plant taxonomy. The voucher sample of A. nilotica has been placed in the herbal collection in the Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Egypt.
Healthy and fresh flowers were collected and washed with clean water. Then, they were dried at 50 • C until a constant weight was obtained. The ground flowers were extracted using methanol via rotary evaporator. The obtained extract was weighed and stored at 5 • C in a refrigerator until further analysis.

HPLC Analysis of Acacia nilotica Flower Extract
Once the extraction process finished, 5 µL of the A. nilotica flower extract was inserted into the HPLC apparatus (Agilent 1260 series, Agilent Technologies, Santa Clara, CA, USA). The specifics of the utilized Eclipse C18 column comprised 4.6 mm × 250 mm i.d., 5 µm and 40 • C. Mobile phase included the use of two buffers: A (0.05% trifluoroacetic acid with mili Q water) and B (0.05% trifluoroacetic acid with acetonitrile). The buffers were applied at 0.9 mL/min as a rate of flow. The serial for buffer A, which had a 20 min run for the mobile phase, was 82, 80, 60, 60, 82, 82 and 82% for 0, 0-5, 5-8, 8-12, 12-15, 15-16, 20-20 min. By using an ultraviolet (UV) detector at a wavelength of 280 nm, the contents of phenolic compounds and flavonoids were identified. The quantity of each was documented based on the injected standard compounds [32].

Assessment of Anti-H. pylori Activity of A. nilotica Flower Extract
H. pylori was obtained from Ain Shams University Hospital, Cairo, Egypt as a tested organism to perform these experiments. The in vitro anti-H. pylori activities were detected using a well agar diffusion technique with the following procedures: A volume of 100 µL suspension of H. pylori (1.0 × 10 8 colony forming units (CFUs)/mL) was seeded in Mueller Hinton agar amended with 10% blood and blood products and poured in plates. After solidification, a hole with a diameter of 6 mm was punched aseptically with a sterile cork borer. Then, 100 µL of the A. nilotica flower extract was loaded into the well. The well loaded with DMSO was utilized as the negative control, while the well loaded with clarithromycin (0.05 mg/mL) was utilized as the positive control. The inoculated plates were incubated at 37 • C with specific conditions (microaerophilic with humidity) for three days. Then, the diameter of the visualized inhibition zone was detected [33].

Minimal Inhibitory Concentration (MIC) Experiment
A. nilotica flower extract was subjected to detect its MIC against H. pylori via microdilution broth, utilizing Mueller-Hinton broth containing lysed horse blood. Different serial dilutions containing 0.98 to 1000 µg/mL of A. nilotica flower extract were prepared. Each appropriate dilution of A. nilotica flower extract (200 µL) was dispensed per well of sterile in 96-well polystyrene microtitrate plates. The inoculum of H. pylori from fresh culture was prepared in sterile NaCl (0.85%) to equal the standard of 1.0 McFarland turbidity. A total of 2 µL of H. pylori inoculum was added to each well to obtain a final dose of 3.0 × 10 6 colony forming units)/mL. The plates were incubated under microaerophilic situations (15% CO 2 ) at 35 • C for 3 days. Then, the MIC was evaluated visually, reflecting the whole growth inhibition of H. pylori. H. pylori inoculum without A. nilotica flower extract was used as a positive control, while A. nilotica flower extract without H. pylori inoculum was used as a negative control in each microplate [34].

Minimal Bactericidal Concentration (MBC) Experiment
MBC was performed via sub-culturing 100 mL of the H. pylori culture from each well that presented thorough inhibition of growth, from the last positive and from the growth control, onto the Mueller-Hinton agar plates (5% horse blood). The plates were incubated under microaerophilic situations (15% CO 2 ) at 35 • C for 3 days. Then, the MBC was evaluated visually, reflecting complete inhibition of H. pylori growth by the lowest concentration of A. nilotica flower extract. To detect either the bactericidal or bacteriostatic agent of the A. nilotica flower extract, the ratios of MBC/MIC were estimated. If the MBC/MIC ratio was no higher than four times that of the MIC, the extract possessed bactericidal efficacy [35].

Microtiter Plate Test for Biofilm Quantification
The influence of A. nilotica flower extract on the formation of H. pylori biofilm was assessed in 96-well polystyrene flatbottom plates. Fresh trypticase soy yeast broth inoculated with H. pylori (250 µL containing 10 6 CFU/mL) was transferred to each microplate and was well supported with the different levels of the sub-lethal quantity of MBC (25, 50 and 75%), which was detected in the current investigation. The plates were incubated for 48 h at 37 • C. Then, after finishing this period, the supernatant was removed. The sterile distilled water for each well was then used to remove free-floating cells. Then, the plates were dried for 30 min in the air. The formed H. pylori biofilm was stained by 0.1% aqueous solution of crystal violet for 15 min at 25 • C (room temperature), followed by removal of the excess of crystal violet via sterile distilled water. To completely remove the dye bound to the H. pylori cells, 250 µL of ethanol (95%) was added to each well as a dye-solubilized agent.
Then, all wells were incubated for 15 min, and, via a microplate reader, the absorbance was measured at 570 nm [36].
The absorbance of the media is represented with Blank, the absorbance of H. pylori with treatment is represented with sample, and the absorbance of H. pylori without any treatment is represented with control.

Urease Activity Inhibition Assessment
The examined solution mixture was contained of urea (850 µL), the extract (in the range of 0 to 100 µL) and phosphate buffer (100 mM, pH 7.4) to reach the total value of 985 µL. The enzymatic reactions began with the addition of urease (15 µL). Then, they were measured via detecting the concentration of ammonia after 60 min utilizing 500 µL of solution A (composed of 0.5 g phenol and 2.5 mg of sodium nitroprusside in 50 mL of distilled H 2 O) and 500 µL of solution B (composed of 250 mg NaOH and 820 µL of NaOCl 5% in 50 mL of distilled H 2 O) for 30 min at 37 • C. The activity of uninhibited urease was selected as 100% of the control activity. The absorbance of the reaction mixture was assessed at 625 nm, and, by using the following formula, the enzymatic activity was determined: The absorbance of the flower extract in the existence of urease is represented as T, and the absorbance of the solvent in the existence of urease is represented as C (control) [37].
The concentration that induces an inhibition halfway among the minimum and maximum response of each compound (IC50) was determined to monitor the inhibition influence of different concentrations of flower extract in the assay. Inhibition activity of hydroxyurea was also evaluated as the standard compound.

Estimation of A. nilotica Flower Extract Antioxidant Activity via DPPH Radical Scavenging Method
A. nilotica flower extract was tested for its capacity to scavenge free radicals using 1,1-diphenyl-2-picryl hydrazyl (DPPH). A total of 0.1 mM DPPH solution in ethanol was prepared; then, 1 mL of this solution was added to 3 mL of A. nilotica flower extract in ethanol at various levels (3.9, 7.8, 15.62, 31.25, 62.5, 125, 250, 500 and 1000 g/mL). Only those extracts that were soluble in ethanol were used in this case, and different concentrations of those extracts were created using the dilution method. The mixture was vigorously shaken before being left to stand at room temperature for 30 min. Then, absorbance was determined at 517 nm (UV-VIS Milton Roy), using spectrophotometer. Ascorbic acid was used as the reference standard compound, and three copies of the experiment were run. Using a log dose inhibition curve, the sample's IC 50 value-the amount of A. nilotica flower extract necessary to inhibit 50% of the DPPH free radical-was determined [24]. The following equation was used to estimate the percentage of the DPPH scavenging outcome: where A0 is the absorbance of the control reaction and A1 is the absorbance in the presence of A. nilotica flower extract or ascorbic acid.

Viability Assay for the Evaluation of the Cytotoxicity of A. nilotica Flower Extract
Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).
All cells were sub-cultured twice a week and kept at 37 • C in a humid environment with 5% CO 2 . Cells were seeded in a 96-well plate at a density of 1 × 10 4 cells per well containing growth medium (100 µL). Different concentrations of A. nilotica flower extract were added to the fresh medium after 24 h of cell seeding. Serial two-fold dilutions of A. nilotica flower extract were added to reach the confluency in 96-well flat-bottomed microtiter plates (Falcon, NJ, USA) utilizing a multichannel pipette. Cells were then incubated at 37 • C in a humid environment with 5% CO 2 for a time of 24 h. Control cells were incubated without A. nilotica flower extract and with or without DMSO. Three wells were utilized for each dose of the A. nilotica flower extract. At the end of the incubation period, the cultivated medium was aspirated. Then, 1% of the crystal violet solution (0.5% w/v crystal violet and 50% methanol made up to volume with ddH 2 O, followed by filtration using Whatmann No.1 filter paper) was added to each well for 30 min. The crystal violet solution was then aspirated. Then, the plates were washed using tap water to remove the excess crystal violet. An amount of 30% glacial acetic acid was added to all wells and mixed carefully. Then, the absorbance of the plates was recorded at 490 nm, and the contents were gently shaken onto the microplate reader (TECAN, Inc.), to detect the yield of viable cells. The optical density was measured with the microplate reader (SunRise, TECAN, Inc., Albany, NY, USA) to determine the viable cells percentage, which was calculated using the following formula: where ODt is the mean optical density of wells exposed to the A. nilotica flower extract, and ODc iss the mean optical density of control cells [38].
The relation among living cells and A. nilotica flower extract concentration was plotted to find the survival curve of the cancer cell line after being exposed to A. nilotica flower extract. From graphic plots of the dose response curve for each concentration, the 50% inhibitory concentration (IC 50 ), or the concentration required to have toxic effects in 50% of intact cells, was calculated using the Graphpad Prism software (San Diego, CA, USA). All investigations were performed in triplicate [39]. Vinblastine sulfate was applied as a positive control against cancer cells under the same experimental conditions.

Experimental Docking Study
Computer research has been used to determine the structural and chemical properties of this particular group of molecules that may have an effect on the apoptotic process. In this study, we looked at the positions of the inhibitor ferulic acid in the H. pylori (4HI0) binding site using molecular docking modeling (MOE) 2019.0102 program. The receptor structures were obtained directly from Protein Data Bank (https://www.rcsb.org/, accessed on 19 July 2022). The downloaded structures were then prepared for docking by removing all water molecules and other metal ions or ligands. The primary chain was docked, and the selected chain was then fixed and protonated utilizing the tools for structure preparation. In order to build the dummy sites that served as the binding pocket, the MOE site finder generated the active binding sites. The studied compounds were minimized and optimized for the docking process [40]. The dock scoring in MOE software was calculated using the London dG scoring function and refined using two different methods. The best five conformations that were presented in the crystal structure with a lower RMSD value were predicted by the docking process. Using the visualizing program PyMol, the complexes were examined for interactions and their 3D images were captured. Additionally, the RMSD and RMSD-refine fields were used to compare the results of pose-with-pose in the co-crystal ligand position prior to and after modification, respectively.

Statistical Study
All statistical studies have been expressed as mean ± standard error (SD) and the outcomes were established at least three times.

Phytochemical Constituents
A. nilotica flower ( Figure 1A) extract was subjected to HPLC analysis, and the incidence of different phenolic and flavonoid constituents with different retention times, concentrations, area and area % ( Figure 1B and Table 1), and chemical formulas was determined ( Figure 2). Three compounds were recognized in the extract with great quantity: 5451.04, 4572.26 and 3733.37 µg/mL for ferulic acid, chlorogenic acid and quercetin, respectively. Other detected compounds such as rutin (2393.13 µg/mL) and gallic acid (2116.77 µg/mL) were reported with moderate concentrations. Cinnamic acid (69.72 µg/mL), hesperetin (121.39 µg/mL) and methyl gallate (140.45 µg/mL) were distinguished with the lowest quantity. Moreover, other compounds in the extract, including catechin, caffeic acid, syringic acid, pyro catechol, ellagic acid, apigenin daidzein and coumaric acid were detected. Unfortunately, eight unidentified compounds were present in the extract due to the defect of the standard compounds in the HPLC system. On the other hand, some compounds including vanillin, naringenin and kaempferol were not found in the extract. There are differences in the detected phenolic and flavonoid compounds according to the organ, the geographical location of the cultivation area, the extraction methods and the nutritional and climatic condition of the plant. Sayed et al. [41] recorded the presence of catechin, catechin 7-O-gallate, gallic acid, naringenin 7-O-β-glucopyranoside and quercetin in A. nilotica flower extract. The major detected compounds in A. nilotica ripe fruits were gallic acid methyl ester, followed by catechin and catechin-7-gallate [42]. In another recent study, Kaur et al. [4] studied the phytoconstituents of A. nilotica bark extract via HPLC, showing the existence of numerous phenolic constituents such as ferulic acid, myricetin, kaempferol, rutin, quercetin, gallic acid, betulin, catechin and epicatechin. The extracted A. nilotica flowers with aqueous methanol reflected the presence of gallic acid, catechin, 7-Galloyl catechin, quercetin 3-O-rhanmnopyranosyl (1→6) glucopyranoside, quercetin-3-O-α-Lrhamnopyranoside, quercetin 3-O-β-glucopyranoside and quercetin [29]. Several studies have focused on the biological activities of the natural phenolic and flavonoid molecules in the fields of pharmaceutics and nutrition. Most of the detected compounds in the extract have been shown to have anticancer, antimicrobial, antioxidant, anti-inflammatory and anti-diabetic properties, but these were illustrated in studies that were conducted on other plants. Yersinia enterocolitica was inhibited by chlorogenic acid [43]. Quercetin exhibited therapeutic potential for treating inflammation, Alzheimer, diabetes and cancer, showing some antioxidant activities [44,45]. Rutin was also reported in inflammation and thrombosis treatments [46].

Anti-Helicobacter Pylori Activity of A. nilotica Flower Extract
Inhibitory activity was attributed to A. nilotica flower extract against H. pylori with an inhibition zone of 31 mm compared to the clarithromycin of 21.67 mm as a positive control (Table 2 and Figure 3A,B). Clarithromycin was selected because it is a broad-spectrum antibiotic that is utilized for the treatment of a wide variety of infections caused by bacteria, including H. pylori infection. Clarithromycin inhibits bacteria via binding to the subunit 50S, leading to the inhibition of protein synthesis. Excellent MIC and MBC were recorded in the A. nilotica flower extract with 7.8 µ g/mL and 15.62 µg/mL, respectively; however, both the MIC and the MBC of the positive control were 31.25 µ g/mL. Via the calculation of the MBC/MIC index, it is clear that the A. nilotica flower extract is determined to be a bactericidal agent due to MBC/MIC values that were less (2.0) than the value of the MIC in the positive control, not exceeding four times that of the positive control MIC (Table 2). Amin et al. [25] reported that the MIC of Helicobacter pylori was found to be 8 μg/mL with the treatment of A. nilotica extract. According to Auwal et al. The inhibitory mechanism of phenolic and flavonoid compounds against pathogenic microorganisms has been elucidated in a number of studies [48]. Asha et al. [49] reported that dihydrofolate reductase, DNA gyrase and protein synthesis in H. pylori were inhibited by a flavonoid-rich plant extract. One of the virulence factors, urease of H. pylori, was also inhibited by definite phenolic compounds [50]. Campos et al. [51] studied the antibacterial activity mechanism of ferulic, p-coumaric and caffeic acids and mentioned the capability of these compounds to interfere with bacterial membrane integrity. Flavonoids such as naringenin, taxifolin and eriodictyol can also interact with several enzymes responsible for the creation of bacterial cell membrane precursors and those responsible for the elongation of fatty acid cycle in Enterococcus faecalis [52]. In another study, methicillin-resistant Staphylococcus aureus peptidoglycan and cell wall biosynthesis were damaged as a result of the exposure to sophoraflavanone B [53].
Moreover, anti-biofilm activity (%) with the increment of the MBC of the A. nilotica flower extract against H. pylori showed 70.38, 82.29 and 94.22% using 25%, 50% and 75% of MBC, respectively. In the control, the anti-biofilm activity was 27.12% ( Figure 3C). A plot of the absorbance versus the levels of MBC utilized is visualized (Figure3D). The color change in the stained biofilm in the microtiter plate was dependent on the viability of the biofilm ( Figure 3E). Several bacterial species, such as Bacillus subtilis, B. cereus, B. megaterium, S. aureus, B. aryabhattai, Pseudomonas putida, Serratia marcescens, Escherichia coli and K. pneumoniae, were impacted by A. nilotica leaf extracts [30].

Anti-Helicobacter Pylori Activity of A. nilotica Flower Extract
Inhibitory activity was attributed to A. nilotica flower extract against H. pylori with an inhibition zone of 31 mm compared to the clarithromycin of 21.67 mm as a positive control (Table 2 and Figure 3A,B). Clarithromycin was selected because it is a broad-spectrum antibiotic that is utilized for the treatment of a wide variety of infections caused by bacteria, including H. pylori infection. Clarithromycin inhibits bacteria via binding to the subunit 50S, leading to the inhibition of protein synthesis. Excellent MIC and MBC were recorded in the A. nilotica flower extract with 7.8 µg/mL and 15.62 µg/mL, respectively; however, both the MIC and the MBC of the positive control were 31.25 µg/mL. Via the calculation of the MBC/MIC index, it is clear that the A. nilotica flower extract is determined to be a bactericidal agent due to MBC/MIC values that were less (2.0) than the value of the MIC in the positive control, not exceeding four times that of the positive control MIC (Table 2). Amin et al. [25] reported that the MIC of Helicobacter pylori was found to be 8 µg/mL with the treatment of A. nilotica extract. According to Auwal et al. This investigation showed the importance of A. nilotica flower extract as an anti-H. pylori agent and its applications in the treatment of H. pylori ulcers. The inhibitory mechanism of phenolic and flavonoid compounds against pathogenic microorganisms has been elucidated in a number of studies [48]. Asha et al. [49] reported that dihydrofolate reductase, DNA gyrase and protein synthesis in H. pylori were inhibited by a flavonoid-rich plant extract. One of the virulence factors, urease of H. pylori, was also inhibited by definite phenolic compounds [50]. Campos et al. [51] studied the antibacterial activity mechanism of ferulic, p-coumaric and caffeic acids and mentioned the capability of these compounds to interfere with bacterial membrane integrity. Flavonoids such as naringenin, taxifolin and eriodictyol can also interact with several enzymes responsible for the creation of bacterial cell membrane precursors and those responsible for the elongation of fatty acid cycle in Enterococcus faecalis [52]. In another study, methicillin-resistant Staphylococcus aureus peptidoglycan and cell wall biosynthesis were damaged as a result of the exposure to sophoraflavanone B [53].   In this study, anti-Helicobacter pylori by a A. nilotica flower extract was assessed and the possible inhibitory influence on its connected urease was also documented. Urease inhibition (%) increased with the increased concentration of the flower extract and reached 76.6% at 1000 µ g/mL. The recorded IC50 was 67.4 µ g/mL (Table 3). Zhou et al. [54] reported the inhibition of H. pylori urease by almatine from Coptis chinensis, in a dose-dependent manner, with IC50 values of 0.53 ± 0.01 mM, in comparison to acetohydroxamic acid, a well-known urease inhibitor (0.07 ± 0.01 mM). Gastric urease permits the H. pylori to inhibit the acidic stomach and functions as a biosign for the occurrence of H. pylori. One of the virulence factors of H. pylori was associated with urease to create the environment's alkaline by transforming urea into ammonia and CO2 [55]. Table 3. Effect of different concentrations of flower extract on urease activity.

Concentration (µg/mL) Flower Extract OD Mean
Urease Inhibition % ±SD 1000 0.172 76.6 0.003 Moreover, anti-biofilm activity (%) with the increment of the MBC of the A. nilotica flower extract against H. pylori showed 70.38, 82.29 and 94.22% using 25%, 50% and 75% of MBC, respectively. In the control, the anti-biofilm activity was 27.12% ( Figure 3C). A plot of the absorbance versus the levels of MBC utilized is visualized ( Figure 3D). The color change in the stained biofilm in the microtiter plate was dependent on the viability of the biofilm ( Figure 3E). Several bacterial species, such as Bacillus subtilis, B. cereus, B. megaterium, S. aureus, B. aryabhattai, Pseudomonas putida, Serratia marcescens, Escherichia coli and K. pneumoniae, were impacted by A. nilotica leaf extracts [30].
In this study, anti-Helicobacter pylori by a A. nilotica flower extract was assessed and the possible inhibitory influence on its connected urease was also documented. Urease inhibition (%) increased with the increased concentration of the flower extract and reached 76.6% at 1000 µg/mL. The recorded IC 50 was 67.4 µg/mL (Table 3). Zhou et al. [54] reported the inhibition of H. pylori urease by almatine from Coptis chinensis, in a dose-dependent manner, with IC 50 values of 0.53 ± 0.01 mM, in comparison to acetohydroxamic acid, a well-known urease inhibitor (0.07 ± 0.01 mM). Gastric urease permits the H. pylori to inhibit the acidic stomach and functions as a biosign for the occurrence of H. pylori. One of the virulence factors of H. pylori was associated with urease to create the environment's alkaline by transforming urea into ammonia and CO 2 [55].

Molecular Docking of Ferulic Acid with 4HI0 Protein of H. pylori
Ferulic acid, which was the highest detected compound in the A. nilotica flower extract, was investigated against 4HI0 protein of H. pylori. The goal of molecular docking, an optimization issue, is to identify the ligand binding mode that has the lowest potential energy. The target binding site's coordinate space was sampled during docking, and each potential ligand, which had been posed within that site, was scored. The ligand posture with the highest score was then used to forecast the binding mode for that chemical. A 2D molecular structure of ferulic acid has been assigned to the MOE (Molecular Operating Environment), which was designed to visualize the active sites of the complexes (proteinligand) ( Figure 4A-F). The ligand was arranged and rendered using an improved version of the 2D algorithm layout representation, and the protein residues were arranged around it to indicate spatial proximity bonds. The obtained results revealed that ferulic acid was a good inhibitor for the (4HI0) enzyme to slow the progression of H. pylori. Ferulic acid has been found to interact with the residue's SER 139 active site via the O 29 atom and had a low energy score (−5.58 Kcal/mol), which was important for their biological activity (Tables 4 and 5). The increased negative score of the free binding energy in the current study confirmed the mechanism of bacterial growth inhibition by ferulic acid. This result was in agreement with recent studies [24,56].
The molecular docking of natural constituents such as chlorogenic acid and pyrocatechol was reported with the H. pylori (4HI0) protein in a recent study [24], which supported the efficacy of these constituents against H. pylori growth. Other investigations also documented the application of the molecular docking of natural ingredient interactions with the E. coli 7C7N protein (value of energy −6.04 kcal mol −1 ) [40] and Proteus vulgaris [2].
in the current study confirmed the mechanism of bacterial growth inhibition by ferulic acid. This result was in agreement with recent studies [24,56].
The molecular docking of natural constituents such as chlorogenic acid and pyrocatechol was reported with the H. pylori (4HI0) protein in a recent study [24], which supported the efficacy of these constituents against H. pylori growth. Other investigations also documented the application of the molecular docking of natural ingredient interactions with the E.coli 7C7N protein (value of energy −6.04 kcal mol −1 ) [40] and Proteus vulgaris [2].     (E) (F) Figure 4. Molecular docking process of ferulic acid with 4HI0. The interaction between ferulic acid and active sites of 4HI0 protein (A). The most likely binding conformation of ferulic acid and the corresponding intermolecular interactions are identified (B). Molecular surface of ferulic acid with 4HI0 (C). The contact preference of ferulic acid with 4HI0 (D). Interaction potential of ferulic acid with 4HI0 (E) and the electrostatic map of ferulic acid with 4HI0 (F).   S, the final score (score of the last stage that was not set to none); rmsd, the root-mean-square deviation of the pose, in Å , from the original ligand; rmsd_refine, the root-mean-square deviation among the pose before refinement and the pose after refinement; E_conf, the energy of the conformer; E_place, the score from the placement stage; E_score 1 and E_score 2, the score from rescoring stages 1 and 2, respectively; E_refine, the score from the refinement stage, which is determined to be the totality of the solvation energies and van der Waals electrostatics, according to the generalized Born solvation model.

Antioxidant Activity A. nilotica Flower Extract
The A. nilotica flower extract presented appropriate antioxidant activity depending on the extract concentration. As the concentration increased, the antioxidant properties of the extract increased. For instance, at 15.63, 62.50, 250 and 1000 µ g/mL, the DPPH scavenging percentage was 42.3, 52.6, 65.5 and 80.6%, respectively, with an IC50 of 36.74 µ g/mL ( Table 6). All findings of the antioxidant properties were judged using standard drug ascorbic acid, which reflected an IC50 of 4.08 µ g/mL. The promising antioxidant activity was associated with the A. nilotica flower extract content of the phenolic and flavonoids constituents. In the current study, the antioxidant activity of A. nilotica flower S, the final score (score of the last stage that was not set to none); rmsd, the root-mean-square deviation of the pose, in Å , from the original ligand; rmsd_refine, the root-mean-square deviation among the pose before refinement and the pose after refinement; E_conf, the energy of the conformer; E_place, the score from the placement stage; E_score 1 and E_score 2, the score from rescoring stages 1 and 2, respectively; E_refine, the score from the refinement stage, which is determined to be the totality of the solvation energies and van der Waals electrostatics, according to the generalized Born solvation model.

Antioxidant Activity A. nilotica Flower Extract
The A. nilotica flower extract presented appropriate antioxidant activity depending on the extract concentration. As the concentration increased, the antioxidant properties of the extract increased. For instance, at 15.63, 62.50, 250 and 1000 µ g/mL, the DPPH scavenging percentage was 42.3, 52.6, 65.5 and 80.6%, respectively, with an IC50 of 36.74 µ g/mL ( Table 6). All findings of the antioxidant properties were judged using standard drug ascorbic acid, which reflected an IC50 of 4.08 µ g/mL. The promising antioxidant activity was associated with the A. nilotica flower extract content of the phenolic and fla- S, the final score (score of the last stage that was not set to none); rmsd, the root-mean-square deviation of the pose, in Å , from the original ligand; rmsd_refine, the root-mean-square deviation among the pose before refinement and the pose after refinement; E_conf, the energy of the conformer; E_place, the score from the placement stage; E_score 1 and E_score 2, the score from rescoring stages 1 and 2, respectively; E_refine, the score from the refinement stage, which is determined to be the totality of the solvation energies and van der Waals electrostatics, according to the generalized Born solvation model.

Antioxidant Activity A. nilotica Flower Extract
The A. nilotica flower extract presented appropriate antioxidant activity depending on the extract concentration. As the concentration increased, the antioxidant properties of the extract increased. For instance, at 15.63, 62.50, 250 and 1000 µ g/mL, the DPPH scavenging percentage was 42.3, 52.6, 65.5 and 80.6%, respectively, with an IC50 of 36.74 µ g/mL ( Table 6). All findings of the antioxidant properties were judged using standard drug ascorbic acid, which reflected an IC50 of 4.08 µ g/mL. The promising antioxidant activity was associated with the A. nilotica flower extract content of the phenolic and fla- S, the final score (score of the last stage that was not set to none); rmsd, the root-mean-square deviation of the pose, in Å, from the original ligand; rmsd_refine, the root-mean-square deviation among the pose before refinement and the pose after refinement; E_conf, the energy of the conformer; E_place, the score from the placement stage; E_score 1 and E_score 2, the score from rescoring stages 1 and 2, respectively; E_refine, the score from the refinement stage, which is determined to be the totality of the solvation energies and van der Waals electrostatics, according to the generalized Born solvation model.  (Table 6). All findings of the antioxidant properties were judged using standard drug ascorbic acid, which reflected an IC 50 of 4.08 µg/mL. The promising antioxidant activity was associated with the A. nilotica flower extract content of the phenolic and flavonoids constituents. In the current study, the antioxidant activity of A. nilotica flower extract was better than using other parts of the plant, as shown in comparison to some previous studies. For example, Subhaswaraj et al. [57] studied the antioxidant activity A. nilotica leaf extract and showed an IC 50 of 75.157 µg/mL. Polyphenols from the extract of A. nilotica ripe fruits demonstrated an IC 50 of 41.91 µg/mL [42]. Another study showed that catechins, a constituent of the A. nilotica flowers, could show antioxidative potential [10].

Anticancer of A. nilotica Flower Extract
The aerial part extracts of Acacia hamulosa, A. salicina, A. laeta and A. tortilis displayed a moderate effect against cancer proliferation in kidney, breast and liver cells [19]. In the current study, the flower extract of A. nilotica exhibited antitumor potential against HepG-2 cells with different levels dependent on the concentration of the extract. A promising result was obtained at a high concentration, in which the inhibitory activity was 91.26% and 96.73% at 500 µg/mL of the flower extract and Vinblastine sulfate against HepG-2 cells, respectively ( Table 7). The IC 50 was 176.15 µg/mL against HepG-2 cells, while the IC 50 was 395.30 µg/mL against HFB-4 cells, which reflects its safe utilization against cancer cells. However, several studies reported the anticancer properties of natural ingredients, but, after reviewing these studies, it appeared that anticancer potential depended on chemical structure, the type of the ingredients and concentration and genomes of tumor cells [29,58]. These remarks coincided with other reports using different parts of A. nilotica [59]. Different IC 50 values of A. catechu fruit extract ranging from 9.7 to 42.8 µg/mL were reported against human cancer cell lines such as those of MCF-7, A549 and THP-1 cells [28]. In a recent study, the anticancer effects of A. nilotica with IC 50 values of 59.3 µg/mL and 96.9 µg/mL were observed against A549 and MCF-7 cells, respectively, while less anticancer effects were observed against THP-1 cells with an IC 50 of more than 100 µg/mL [29]. The influence of A. nilotica flower extract on normal cells was parallel with Revathi et al. [30], in which the A. nilotica leaf extracts documented the anticancer activity of the extracts against Hep-2 (cervical cancer) and MDA-MB-231 (breast cancer) and showed sparing effects on normal Vero cells. As mentioned from the HPLC analysis, ferulic acid was the main component detected in the A. nilotica flower extract. The mechanisms of ferulic acid cytotoxicity against cancer cells was studied, from which autophagy and apoptosis of HepG2 were observed with the disruption of nucleoli, morphological alterations, cell proliferation decline, mitochondrial membrane potential decrement, and an increased % of cells in the S phase [60]. In a review report [61], cancer cells treated with ferulic acid characterized with DNA fragmentation lowered the rate of protein kinase phosphorylation. Sundarraj et al. [62] reported that the MCF-7 cell cycle at the G2/M phase was blocked as a result of the exposure to a leaf extract of A. nilotica. Another species, namely, Acacia catechu, also arrested the human colon cancer cell cycle at this phase and showed a decline in the potential mitochondrial membrane [63].

Conclusions
This study offered additional scientific support to the utilization of A. nilotica flower extract to treat H. pylori-related gastrointestinal infections. A. nilotica flower extract might be a helpful treatment for peptic and gastritis ulcers induced by H. pylori infection and other urease-related illnesses. The results of this study suggest that the A. nilotica flower extract, due to its considerable antioxidant potential, might play a role in protection via the avoidance of lipid peroxidation generated by free radicals. Furthermore, based on the obtained findings, further studies must be conducted to document the biological effects of A. nilotica flower extract in vivo, and detailed mechanistic studies are required to validate these biological applications.