Amelogenin-Derived Peptide (ADP-5) Hydrogel for Periodontal Regeneration: An In Vitro Study on Periodontal Cells Cytocompatibility, Remineralization and Inflammatory Profile

A relevant alternative to enamel matrix derivatives from animal origin could be the use of synthetic amelogenin-derived peptides. This study aimed to assess the effect of a synthetic amelogenin-derived peptide (ADP-5), alone or included in an experimental gellan–xanthan hydrogel, on periodontal cell behavior (gingival fibroblasts, periodontal ligament cells, osteoblasts and cementoblasts). The effect of ADP-5 (50, 100, and 200 µg/mL) on cell metabolic activity was examined using Alamar blue assay, and cell morphology was assessed by confocal imaging. An experimental gellan–xanthan hydrogel was then designed as carrier for ADP-5 and compared to the commercial gel Emdogain®. Alizarin Red was used to determine the periodontal ligament and cementoblasts cell mineralization. The inflammatory profile of these two cells was also quantified using ELISA (vascular endothelial growth factor A, tumor necrosis factor α, and interleukin 11) mediators. ADP-5 enhanced cell proliferation and remineralization; the 100 µg/mL concentration was more efficient than 50 and 200 µg/mL. The ADP-5 experimental hydrogel exhibited equivalent good biological behavior compared to Emdogain® in terms of cell colonization, mineralization, and inflammatory profile. These findings revealed relevant insights regarding the ADP-5 biological behavior. From a clinical perspective, these outcomes could instigate the development of novel functionalized scaffold for periodontal regeneration.


Introduction
Periodontal disease is a highly prevalent infectious disease that induces inflammation of periodontal tissues until their destruction [1]. Periodontal inflammation also has systemic consequences that can aggravate certain pathologies such as diabetes, cardiovascular diseases, and rheumatoid arthritis [2,3]. Moreover, it could be correlated with pregnancy complications such as underweight and preterm infants [4,5]. Periodontal therapy can successfully stop the disease progression by targeting the microbial etiology of the disease; this allows the recovery of periodontal health and also can improve the conditions of certain pathologies associated with periodontitis [6,7]. However, one of the remaining challenges is the regeneration of the destroyed tissues, i.e., new cementum, periodontal ligament, and bone coronal to the base of the defect [8]. This regeneration through the
The scanning electron microscopy (SEM) analysis was performed to visualize the ADP-5 structure. The powder of peptide was sputter coated with copper (10 ng) and observed under SEM (SEM, FEI-Quanta 250-Thermo Fisher Scientific, Illkirch-Graffenstaden, France).

Incorporation of the ADP-5 Peptide in the GX Hydrogel
GX powder was formed from a mixture of Gellan (P8169, Phytagel™, Sigma Aldrich, Saint-Quentin-Fallavier, France) and Xanthan powders (G1253, Sigma Aldrich, France) at a ratio of 9:1 wt%. All the hydrogels were prepared from this mixture with cold PBS: Phosphate-Buffered Saline (14190144: Thermo Fisher Scientific, Illkirch-Graffenstaden, France) buffer solution; a 30 s vibration using the vibrator Softly Satelec (Henry Schein, Limonest, France) was then applied to enable the hydrogel crosslinking. Several gel densities were tested and the GX hydrogel at the density of 3% was selected to be used in this study due to its mechanical properties and comparable clinical handling to the commercial Emdogain ® (EMD: Straumann, Basel, Switzerland). The ADP-5 peptide was incorporated (mixed with the GX powders directly) in the selected GX hydrogel at the concentrations of 100 and 200 µg/mL. The GX/ADP-5 mixture was first prepared using PBS, and then immerged in the culture media before the cell contact.
The list of tested samples used in the first and the second investigation steps, including synthetic peptide, and experimental and commercial gels, are shown in Table 1. The first investigation refers to the assessment of ADP-5 peptide alone and the second investigation refers to the assessment of ADP-5 factionalized experimental GX hydrogel.

Cell Metabolic Activity
Cell metabolic activity was evaluated using the Alamar blue assay (DAL1100, Thermo Fisher Scientific, France), which is colored blue in its oxidized state and becomes pink when reduced to the resorufin by metabolically active cells. Briefly, cells were seeded on 24-well culture plates at 104 cells/mL, according to the international organization for standardization (ISO) 10993-norm recommendations [23]. They were cultured in a regular medium, with several conditions in two experimental conditions. In the first part, the four periodontal cells (HGF, hPDL, MG63, and OCM.30) were cultured in direct contact with the synthetic ADP-5 at the following concentrations: 5, 10, 50, 100, and 200 µg/mL. In the second part of the experimental protocol, the same cell types were cultured in the presence of either GX hydrogel, or GX hydrogel with 100 µg/mL (GX-100) or 200 µg/mL (GX-200) ADP-5 peptide, or 100 µg/mL or 200 µg/mL commercial gel Emdogain ® (diluted in the culture media) (Emd ® 100 and Emd ® 200, respectively) ( Table 1). After 1, 3, and 7 days of contact, Alamar blue solution was added directly into wells at the final concentration of 10% v/v and plates were incubated at 37 • C for 7 h. The amount of resorufin formed was determined by measuring absorbance intensity (570/600 nm), using an absorbance plate reader Infinite (Nanoquant infinite M200 pro, Tecan group Ltd., France). The results were expressed through the percentage of viability amount to 100% of viability of the control cells (cells without peptide for the first investigation step and cells without peptide or gel for the second investigation step).

Cell Morphology
The cell morphology was observed using confocal microscopy to evaluate the incidence of 7 days of direct contact with the tested peptide at the 50 and 100 µg/mL concentration (for hPDL and MG63). Fluorescence staining was performed to observe the formation and the organization of stressed fibers and morphological changes. Cells were washed three times with PBS. Then, they were fixed for 30 min by incubating in 3.7% formaldehyde (10426730, Thermo Fisher Scientific, France) in PBS, followed by further washing. The cells were permeabilized with 1% triton ×100 (×100, Sigma Aldrich, Saint-Quentin-Fallavier, France) in PBS, and then blocked with 1% Corning bovine serum albumin (354331: Thermo Fisher Scientific, Illkirch-Graffenstaden, France) in PBS. Actin microfilaments were stained by Alexa Fluor™ 488 Phalloidin (A12379, Thermo Fisher Scientific, France) at a 1:100 ratio at room temperature to visualize cell actin filaments (green fluorescence). Cell nuclei were identified using propidium iodide (P3566, Thermo Fisher Scientific, France) at a 1:3000 ratio at room temperature (red fluorescence) to visualize nuclei. Supercontinuum whitelight laser was used to excite Alexa Fluor ® 488 and propidium iodide. Acquisitions were collected sequentially (green fluorescence/red fluorescence) to avoid potential crosstalk between the two channels. The resulting stained cells, in 1% bovine serum albumin in PBS, were examined under a confocal laser scanning microscope (CLSM LEICA SP5X Leica, Wetzlar, Germany).

Mineralization Ability of hPDL and OCM.30 Cells
Alizarin red staining (ARS) was used to assess matrix mineralization. In the first step of the investigation, the ARS assay was carried out following the contact of ADP-5 alone, at the concentration of 100 µg/mL and 200 µg/mL, after 7 and 14 days for both hPDL and OCM.30 cells. In the second part of the investigation, hPDL cells were subjected to 100 µg/mL and 200 µg/mL GX-ADP-5 or Emdogain ® (100 µg/mL and 200 µg/mL). The culture media were changed every 3 to 4 days.
For the ARS analysis, cells were firstly fixed using formaldehyde (3.7% in PBS, 30 min) and washed with deionized water. Then, 40 mM of ARS solution (pH 4.2) was added into the 24-well plates. The cells were incubated at room temperature for 40 min, then washed 3 times with deionized water and viewed under an optical microscope (CKX41, Olympus, France). For quantitative calcium analysis (semi-quantification) of mineralized matrix nodules generated from hPDL and OCM.30, the cells were treated with 10% cetylpyridinium chloride solution (C0732: Sigma-Aldrich, Saint-Quentin-Fallavier, France) for 30 min at room temperature to dissolve and release the calcium-combined ARS. The optic density values were read at 560 nm, which represented the relative quantity of deposited calcium in the mineralization nodules. The experiments were repeated at least 3 times (n = 9).

Inflammatory Mediators Production
ELISA assays were used on hPDL and OCM.30 cells to quantify the production of three inflammatory mediators: a growth factor, vascular endothelial growth factor A (VEGF-A), and two inflammatory cytokines, tumor necrosis factor alpha (TNF-α) and interleukin 11 (IL-11), involved in inflammatory reaction periodontitis [24,25]. These assays were made at 3 days following direct contact of each two-cell types with GX, GX-100, GX-200, Emd ® 100, and Emd ® 200. All cell lysates were subjected to ELISA for inflammatory mediators by applying ELISA kits: human (for hPDL) and mouse (for OCM.30) VEGF-A (KHG011, ThermoFisher Scientific), TNF-α (BMS223HS, ThermoFisher Scientific, France), and IL-11 (human EHIL11 and mouse EMIL11, ThermoFisher Scientific, France). Each ELISA was performed according to the manufacturer's instruction. The experiment was performed in triplicate and repeated three times (n = 9); the data were compared to a standard curve. Absorption measurements were performed at 450 nm (Nanoquant infinite M200 pro, Tecan group Ltd., Lyon, France).

Statistical Analyses
Statistical analyses were performed by using one-way analysis of variance (ANOVA). An initial comparison was made between the control group (cells without any treatment) and the tested groups (cells in contact with ADP-5 alone or within the experimental gel versus Emdogain ® ). Then, the Mann-Whitney test and the Wilcoxon signed rank tests were used for comparison between the different investigated groups. The level of significance was set at * p < 0.05 and ** p < 0.001.

ADP-5 Structure by SEM
SEM analysis (Figure 1) demonstrates the morphology of ADP-5 peptide. Regular surface morphology of the peptide can be shown. of significance was set at * p < 0.05 and ** p < 0.001.

ADP-5 Structure by SEM
SEM analysis (Figure 1) demonstrates the morphology of ADP-5 peptide. Regular surface morphology of the peptide can be shown.

Effect of ADP-5 Alone on Cell Viability
The results of cell metabolic activity, comparing the different concentrations of ADP-5 on the four periodontal cell types, are shown in Figure 2. A slight and significant decrease in cell viability was observed for HGF cells from the concentration of 50 μg/mL, whatever the contact time point (1, 3, and 7 days), compared to control cells. For the other three cell types (hPDL, OCM.30, and MG63), a cell viability significant enhancement was observed from day 1 of incubation with 50 μg/mL ADP-5 for OCM.30 and hPDL and 100 μg/mL ADP-5 for MG63 cells. hPDL cell viability revealed a significant enhancement at the concentrations of 50 μg/mL and 100 μg/mL; for OCM.30, a significant enhancement in cell viability was only observed at 100 μg/mL ADP-5; for MG63, the three concentrations

Effect of ADP-5 Alone on Cell Viability
The results of cell metabolic activity, comparing the different concentrations of ADP-5 on the four periodontal cell types, are shown in Figure 2. A slight and significant decrease in cell viability was observed for HGF cells from the concentration of 50 µg/mL, whatever the contact time point (1, 3, and 7 days), compared to control cells. For the other three cell types (hPDL, OCM.30, and MG63), a cell viability significant enhancement was observed from day 1 of incubation with 50 µg/mL ADP-5 for MG63 and hPDL and 100 µg/mL ADP-5 for OCM.30 cells. hPDL cell viability revealed a significant enhancement at the concentrations of 50 µg/mL and 100 µg/mL; for OCM.30, a significant enhancement in cell viability was only observed at 100 µg/mL ADP-5; for MG63, the three concentrations (50 µg/mL, 100 µg/mL, and 200 µg/mL ADP-5) induced a significant enhancement in cell viability versus control cells.

Effect of GX Hydrogels Containing ADP-5 Versus Emdogain ® Gels on Cell Viability
The cell metabolic activity demonstrated no cytotoxic effect whatever the gel treatment conditions ( Figure 3). For HGF cells, no statically significant difference was observed among all the tested gel groups. The other cell types exhibited a significant increase in cell metabolic activity in the presence of both GX containing ADP-5 and EMD regardless of the contact time. The most stimulation was found for hPDL following 7 days of contact with both ADP-5 and EMD. This enhancement occurred since the first day of contact at the concentration of 100 µg/mL. For the tested OCM.30 cementoblasts and MG63 osteoblasts cells, a significant increase was observed beginning at the concentration of 100 µg/mL of Emdogain and at 200 µg/mL of GX-ADP-5. It seems that the incorporation of ADP-5 in the GX hydrogel did not affect its active effect regarding the tested four periodontal cells.

Effect of GX Hydrogels Containing ADP-5 Versus Emdogain ® Gels on Cell Viability
The cell metabolic activity demonstrated no cytotoxic effect whatever the gel treatment conditions (Figure 3). For HGF cells, no statically significant difference was observed among all the tested gel groups. The other cell types exhibited a significant increase in cell metabolic activity in the presence of both GX containing ADP-5 and EMD regardless of the contact time. The most stimulation was found for hPDL following 7 days of contact with both ADP-5 and EMD. This enhancement occurred since the first day of contact at the concentration of 100 μg/mL. For the tested OCM.30 cementoblasts and MG63 osteoblasts cells, a significant increase was observed at the concentration of 100 μg/mL of Emdogain and at 200 μg/mL of GX-ADP-5. It seems that the incorporation of ADP-5 in the GX hydrogel did not affect its active effect regarding the tested four periodontal cells.      Figure 5A reveals an important enhancement of the extracellular calcium deposition amount in a time-dependent manner (from 7 to 14 days) in the hPDL treated with 100 µg/mL ADP-5 compared to the untreated hPDL cells. Interestingly, the lowest concentration (100 µg/mL) was more efficient than the highest concentration (200 µg/mL). More mineralization nodules were observed when hPDL cells were interfaced by ADP-5 at the concentration of 100 µg/mL, whatever the time point tested ( Figure 6A). Regarding OCM cells, the Alizarin red quantification ( Figure 5B) demonstrates a significant enhancement in calcium deposition, which is higher in the presence of 200 µg/mL of ADP-5 in a time-dependent manner. At the concentration of 100 µg/mL, a slight but significant increase was also shown with a time-dependent effect. When cells were interfaced by gel groups, the experimental functionalized group GX100 was the most efficient and revealed significant enhancement of the extracellular calcium deposition amount compared to all the tested gel groups ( Figure 5C). Qualitatively, optical images of Alizarin red OCM.30 stained cells ( Figure 6B) illustrate an evident increase in the mineralized nodules in the ADP-5 groups compared to the control groups (cells without ADP-5).

hPDL and OCM.30 Mineralization Ability by Alizarin Red Staining
µ g/mL ADP-5 compared to the untreated hPDL cells. Interestingly, the lowest concentration (100 µg/mL) was more efficient than the highest concentration (200 µg/mL). More mineralization nodules were observed when hPDL cells were interfaced by ADP-5 at the concentration of 100 µ g/mL, whatever the time point tested ( Figure 6A). Regarding OCM cells, the Alizarin red quantification ( Figure 5B) demonstrates a significant enhancement in calcium deposition, which is higher in the presence of 200 µ g/mL of ADP-5 in a timedependent manner. At the concentration of 100 µg/mL, a slight but significant increase was also shown with a time-dependent effect. When cells were interfaced by gel groups, the experimental functionalized group GX100 was the most efficient and revealed significant enhancement of the extracellular calcium deposition amount compared to all the tested gel groups ( Figure 5C). Qualitatively, optical images of Alizarin red OCM.30 stained cells ( Figure 6B) illustrate an evident increase in the mineralized nodules in the ADP-5 groups compared to the control groups (cells without ADP-5). Data are mean ± SD of 3 independent experiments (n = 6). * p <0.05 and ** p <0.001 ** denote a significant difference. Calcium deposition quantification by Alizarin red staining of hPDL (C) cells following hydrogel contact groups (GX containing or not ADP-5 peptide or EMD). Data are mean ± SD of 3 independent experiments (n = 9/group). p < 0.001 ** denotes a significant difference (GX100 group compared to all the tested groups). Data are mean ± SD of 3 independent experiments (n = 6). * p < 0.05 and ** p < 0.001 denote a significant difference. Calcium deposition quantification by Alizarin red staining of hPDL (C) cells following hydrogel contact groups (GX containing or not ADP-5 peptide or EMD). Data are mean ± SD of 3 independent experiments (n = 9/group). ** p < 0.001 denotes a significant difference (GX100 group compared to all the tested groups).

hPDL and OCM.30 Cells Inflammatory Mediator Quantification by ELISA Assay
The inflammatory profile of the two investigated periodontal cells (periodontal ligament cells and cementoblasts) was assessed by quantifying the protein level of three inflammatory factors: VEGF-A, TNF-α, and IL-11. The obtained data are presented in Figure 7. For both tested cell types, both GX hydrogel containing ADP-5 and Emdogain ® increased significantly both VEGF-A and IL-11, while the TNF-α protein amounts were decreased in contact with the two tested gels. Cytokine production remained unaffected when challenged by nonfunctionalized GX hydrogel. For OCM.30 cells, the production of IL-11 was enhanced only in the presence of 200 µg/mL GX-ADP-5.

hPDL and OCM.30 Cells Inflammatory Mediator Quantification by ELISA Assay
The inflammatory profile of the two investigated periodontal cells (periodontal ligament cells and cementoblasts) was assessed by quantifying the protein level of three inflammatory factors: VEGF-A, TNF-α, and IL-11. The obtained data are presented in Figure  7. For both tested cell types, both GX hydrogel containing ADP-5 and Emdogain ® increased significantly both VEGF-A and IL-11, while the TNF-α protein amounts were decreased in contact with the two tested gels. Cytokine production remained unaffected when challenged by nonfunctionalized GX hydrogel. For OCM.30 cells, the production of IL-11 was enhanced only in the presence of 200 µ g/mL GX-ADP-5.

Discussion
Periodontal tissue regeneration is the main aim of current periodontal therapies intended to recover damaged tissues. The periodontium complex structure, with alternating hard and soft tissues, makes its tissue regeneration a challenge. In this context, treatments, associated with periodontal wound healing and tissue regeneration have been extensively investigated [16,[26][27][28]. Among the current periodontal regenerative therapy procedures, Emdogain ® is being widely used in clinical practice [14]. This gel of animal origin is mostly comprised of amelogenins, responsible for mineralization during tooth root formation, and which has inherent cementogenic properties [29,30]. On the other hand, Emdogain ® , as well as guided tissue regeneration (GTR) and other therapies, can often be unpredictable for large defects [31][32][33][34]. Moreover, its animal origin remains a barrier for some patients. In this respect, recent research is directed towards synthetic peptide to overcome some of these limitations; therefore, the use of the ADP-5 synthetic peptide initially identified by Gungormus et al. [18] could provide a relevant answer to these patients and constitutes an alternative to animal sacrifice. ADP-5 peptide was reported to have a beneficial remineralization ability with a potential use for periodontal tissue regeneration. Nonetheless, the ADP-5, in its initial form of powder, needs to be incorporated in a carrier "transporter" to facilitate its use and preserve its active potential in periodontal pockets and in Figure 7. Rate of inflammation mediators production by ELISA after 3 days of cells' contact with GX containing or not ADP-5 and EMD: protein rate of VEGF-A factor (A), TNF-α (B), and IL-11 (C) by hPDL (left side) and OCM.30 (right side) cells. Data are mean value of 3 independent experiments (n = 12/group). * p < 0.05 and ** p < 0.001 denote a significant difference.

Discussion
Periodontal tissue regeneration is the main aim of current periodontal therapies intended to recover damaged tissues. The periodontium complex structure, with alternating hard and soft tissues, makes its tissue regeneration a challenge. In this context, treatments, associated with periodontal wound healing and tissue regeneration have been extensively investigated [16,[26][27][28]. Among the current periodontal regenerative therapy procedures, Emdogain ® is being widely used in clinical practice [14]. This gel of animal origin is mostly comprised of amelogenins, responsible for mineralization during tooth root formation, and which has inherent cementogenic properties [29,30]. On the other hand, Emdogain ® , as well as guided tissue regeneration (GTR) and other therapies, can often be unpredictable for large defects [31][32][33][34]. Moreover, its animal origin remains a barrier for some patients. In this respect, recent research is directed towards synthetic peptide to overcome some of these limitations; therefore, the use of the ADP-5 synthetic peptide initially identified by Gungormus et al. [18] could provide a relevant answer to these patients and constitutes an alternative to animal sacrifice. ADP-5 peptide was reported to have a beneficial remineralization ability with a potential use for periodontal tissue regeneration. Nonetheless, the ADP-5, in its initial form of powder, needs to be incorporated in a carrier "transporter" to facilitate its use and preserve its active potential in periodontal pockets and in different clinical situations. Within this framework, the use of ADP-5 peptide-functionalized hydrogels as a modified bioactive scaffold may represent a relevant solution for periodontal complex regeneration, containing all of the required structural (such as easy handling and gel flexibility) and bioactive properties (such as ADP-5 mineralization ability) and lacking any immunogenic cellular components due to the absence of animal origin.
The concept of the current study was to compare and evaluate the in vitro biological behavior of an experimental hydrogel, based on gellan-xanthan mixture containing ADP-5 peptide. The in vitro behavior of the four representative periodontal cell types (gingival fibroblasts, osteoblasts, periodontal ligament cells, and cementoblasts) was first assessed in contact with ADP-5 alone in terms of cell proliferation, cell morphology, and cell remineral-ization. As a second investigation step, the effect of incorporating the tested ADP-5 into the experimental hydrogel scaffold, was then performed.
The assessment of experimental scaffold in contact with cells is a fundamental first step of biocompatibility and bioactivity evaluation. Cell biological testing should be conducted on specific cells according to the recommendations established by ISO 10993-5 [35]. The clinical applications have to be considered in order to select the suitable cell type regarding the targeted tissue with the aim to mimic as possible in vivo situations [36]. Periodontal ligament cells (hPDL) have been considered as the cell type with the highest potential for periodontal regeneration [37]. This could explain the large use of hPDL cells when assessing the performance of different periodontal therapies [38]. The effect on cell growth of EMD in a gel carrier versus EMD in a liquid carrier and their regulatory role of the inflammatory reaction was also investigated using PDL cells [39]. Moreover, cementoblast (OCM.30) cells were used for the same purpose, such as the potential use of plasminogen activation system as a specific target to control amelogenin-mediated tissue regeneration [40] and the potential autophagy effect of TNF-α and its critical role in cementoblasts differentiation, mineralization, and apoptosis [28,41,42].
The results of the present study indicate that ADP-5 peptide enhanced periodontal cells' metabolic activity and cell spreading when assessed alone; this is in agreement with the results obtained by Gungormus et al. [18] regarding periodontal ligament cells. Moreover, the tested ADP-5 enhanced the behavior of three other investigated periodontal cell types, namely the osteoblasts, cementoblasts, and gingival fibroblasts. When the ADP-5 was incorporated into the experimental gellan-xanthan gel, it was found to behave identically to the commercial Emdogain ® regarding periodontal cells. Emdogain ® has previously demonstrated a unique capacity to promote periodontal cells' mineralization in vitro [43]. The 100 µg/mL concentration induced the best cell response in the presence of the ADP-5 alone, and the 200 µg/mL was the highest concentration used that allowed the release of the active molecules from the experimental functionalized hydrogel. On the other hand, the obtained results demonstrated that this synthetic peptide stimulates the proliferation of cementoblasts, osteoblasts, and especially the periodontal ligament cells, while limiting the proliferation of gingival fibroblasts. This relative inhibition of gingival fibroblast proliferation could be of a major clinical relevance, since it prevents gingival tissue from interfering with periodontal attachment during the regeneration process [29,44]. To the best of our knowledge, this is the first study assessing four representative periodontal cells in contact with the ADP5 peptide. Hoang et al. [45] evaluated the effect of EMD on three cell types (PDL, HGF, and Mg63 cells) on wound healing using an in vitro wound model based on wounded cell monolayers in tissue culture plates. As in the current study regarding the ADP-5 peptide, the authors concluded that EMD stimulated periodontal ligament cell proliferation, which consequently improved periodontal regeneration.
Since stimulation of cell proliferation alone does not accurately substantiate the biological behavior of cells during the regeneration process, mineralization ability, and the inflammatory profile of the two main representative periodontal cells (periodontal ligament as the representative of soft tissue and attach cells and cementoblasts as representative of hard tissue) were also assessed in the current study. Alizarin red S assay demonstrated more mineralization nodules and significant enhancement of the extracellular calcium deposition when cells were interfaced with both ADP-5 alone and ADP-5 functionalized experimental hydrogel compared to control cells. Alizarin red staining assay is commonly used to determine the mineralized nodule formation and quantify calcium deposits at the interface of cells-biomaterials, such as mesoporous bioactive glasses in contact with pulp cells [46], PDL cells after their stimulation with TNF-α [47], primary osteoblasts and PDL cells after contact with EMD [40], and MC3T3-E (mouse osteoblast cell line) when interfaced with strontium-doped bioactive glasses [48].
In the current study, the inflammatory profile was assessed using ELISA by quantifying the protein level to predict the inflammatory response induced by the ADP-5 peptide. The ELISA technique is widely used to quantify mediators [49][50][51][52][53]. Three key inflammatory mediators were investigated: a proinflammatory cytokine (TNF-α), an angiogenic growth factor (VEGF-A), and the anti-inflammatory cytokine (IL-11). It was previously reported that IL-11 is underexpressed in periodontitis [54,55], while TNF-α is overexpressed [55], there is a reduction of VEGF in periodontal disease [56], and these are correlated with the resolution of periodontal inflammation and periodontal tissue healing [57]. In the present study, both experimental GX hydrogel containing ADP-5 and Emdogain ® increased VEGF-A and IL-11 significantly and decreased TNF-α protein amounts. This is consistent with the finding of Almqvist et al. [58]; the authors demonstrated that monocyte-derived macrophages, stimulated by inflammatory agonist LPS, responded to the treatment with Emdogain ® by decreasing the TNF-α proinflammatory activity and enhancing the expression of tissue repair mediators such as VEGF. In a previous study, the production of inflammatory cytokine by monocytes was assessed using cDNA microarrays, and EMD downregulated the expression of genes involved in the early inflammatory phases of wound healing, such as interleukin (IL-6) [40]. Despite the dissimilarities with our study (cell type and methodology), these findings indicated that the decrease in proinflammatory markers (such as TNF-α) in response to the ADP-5 peptide could contribute to the downregulation of the proinflammatory response occurring after the placement of a scaffold during a regeneration process. Villa et al. [59] found that the proinflammatory cytokine secretion from human gingival fibroblasts decreased following treatment with EMD and its peptide fractions. In agreement with the current study, this funding underlined the biological activity of amelogenic peptides on proinflammatory cell behavior.
In the current study, the GX experimental hydrogel was merely developed to provide a proof of concept for the ADP-5 carrier. Therefore, the main limitation of the study was that the experimental hydrogel used is different in composition from the commercial gel Emdogain ® . Additional hydrogel compositions close to the one contained in Emdogain ® could also have been assessed. The characterization of the mechanical properties of the functionalized experimental hydrogel, as well as the kinetic release of ADP-5 from the GX hydrogel, should be assessed and controlled in the future; these could provide additional information regarding the functionalized scaffold potential clinical use.
From a clinical perspective, it will be necessary in future investigations to assess the experimental ADP-5 functionalized gellan-xanthan hydrogel in vivo, especially targeting periodontal regenerative procedures such as intraosseous or furcation lesion regeneration. The storage constraints of the commercial gel Emdogain ® represent a crucial point that may limit its use by dentists, especially those who are geographically isolated. The next step would be to investigate the storage constraints of the ADP-5 functionalized gellan-xanthan hydrogel suggested in the present study.

Conclusions
The present study highlights the superior in vitro biological behavior of the assessed synthetic amelogenin-derived peptide ADP-5, alone and within the resorbable scaffold, based on gellan-xanthan hydrogel. This behavior was revealed by: -Enhanced proliferation, adhesion, and spreading of all periodontal cell types, and especially periodontal ligament cells; -Slight inhibition of human gingival fibroblasts proliferation; -Enhancement in periodontal cell mineralization potential; -Improvement of key inflammatory mediators.
After further investigations, the elaborated bioactive scaffold-based ADP-5 peptide together with GX hydrogel could serve as a potential regenerative material for clinical practice. Moreover, the synthetic animal-free ADP-5 peptide could be used as a bioactive agent to functionalize different scaffolds for periodontal regeneration.