Evaluation of a Dynamic Bioremediation System for the Removal of Metal Ions and Toxic Dyes Using Sargassum Spp.

: This work presents the results obtained in the design and manufacture of a simple, economic and ecological ﬁlter based on Sargassum spp. (Sspp), consisting of the species S. natans and S. ﬂuitans , for the elimination of organic and inorganic toxic substances. The main objective is to make use of Sspp, as the massive amounts of this alga arriving at the Mexican Caribbean coast have caused serious problems over recent years. The toxic substances treated were organic dyes (methyl blue, methyl orange and methyl red) and the metal ion, lead (II). To obtain optimal removal conditions, grinding of the Sspp used, its mass and humidity were evaluated. In the design of the ﬁlter the area, ﬂow rate and the number of layers were evaluated. Removal rates of almost 100%, 65% and 25% were obtained for methylene blue, methyl red and methyl orange respectively, and in the case of lead (II), values up to 95% were obtained. After the tests, the Sspp was characterized, using Fourier Transform Infrared (FTIR) spectroscopy and scanning electron microscopy, showing the presence of the dyes and the ionic species. These results demonstrate the e ﬃ ciency of the dynamic Sspp-based ﬁltration system proposed, which can be industrially scaled for the treatment of water contaminated with these kinds of substances. ﬁlter became clogged due to the absorption of water by the CT- Sargassum spp. Increasing the mass of CT- Sargassum spp. used in the ﬁlter gave greater removal at a faster rate. The diameter of the ﬁlter used and the water ﬂow were also evaluated. In the ﬁrst case, the highest removal was obtained when a 1 inch diameter ﬁlter was used. In the second case, it was observed that as the ﬂow decreases, the removal rate and e ﬃ ciency increase: a ﬂow of 3 mL / s was found to be the optimum rate of ﬂow. With respect to the number of layers (plates), as expected, removal was greater with an increased number of adsorption layers. In this work, a maximum of 4 layers were used. The experiments performed show that the Sargassum spp.-based water ﬁlter system was e ﬃ cient. The e ﬃ ciency and the rate of removal were di ﬀ erent for each substance. The total removal of methylene blue, methyl red and methyl orange was 99%, 68% and 25%, respectively. Regarding lead ions, the removal percentage obtained was 95%. These results demonstrate the ability of CT- Sargassum spp. to trap and remove speciﬁc water-soluble toxic substances from water. This kind of dynamic biosorbent ﬁlter system is therefore an eco-friendly, scalable and cost-e ﬀ ective option for domestic, agricultural and industrial purposes. The future work of the present study is to evaluate the removal of pollutants using real samples taken from e ﬄ uents of rivers, lakes or seas, which contain various pollutants at di ﬀ erent concentrations. Supervision—M.E.; Validation—J.L.L.-M., G.A.M., R.E., A.R.H.-M. and M.E.; Visualization—J.L.L.-M. and G.A.M.; Writing—original draft, J.L.L.-M., R.S., G.A.M., R.E., A.R.H.-M. and M.E.; Writing—review and editing, R.S., R.E. and M.E. All authors have read and agreed to the published version of the


Introduction
The discharge of untreated, or partially treated, wastewaters and industrial effluents into natural ecosystems can create serious issues in the environment as we know it, since these are a source of esthetic degradation, eutrophication and perturbations in aquatic life [1][2][3][4]. Many water pollutants, including organic compounds, especially synthetic azo dyes (-N=N-), come from industries such as pharmaceuticals, food, laboratory printing and textiles [5][6][7]. Most of these are highly carcinogenic, even in minimal amounts [8]. Therefore, the elimination of such pollutants is of great importance to society.
Azo dyes contain molecular aromatic complexes, which makes them difficult to degrade. They are very stable to ultraviolet radiation, oxidizing agents, solar light irradiation and biochemical treatment (e.g., aerobic digestion) [8,9]. Methyl red and orange are carcinogenic water-soluble azo dyes that In this work, the biosorption of lead, methyl orange, methyl red and methylene blue via a simple dynamic filtration system is evaluated. Two species of Sargassum were used, S. natans and S. fluitans, collected on the Mexican Caribbean coast. Results obtained in this work show that the Sargassum species S. natans and S. fluitans have a high adsorption capacity for the azo dyes, in particular for methylene blue, and for lead (II) cations. Sspp also requires a short time to reach equilibrium, suggesting that it could be used as a low-cost biosorbent in industrial-scale, efficient and economic wastewater treatments.

Filter System based on Sargassum spp. (Sspp)
To obtain the optimum filtration system, various parameters were evaluated, such as chemical treatment, grinding and the mass of the Sspp used, filter diameter, the number of layers, the flow rate of the solution and the degree of humidity of the Sspp. All these parameters were evaluated only with methylene blue. The best result obtained in each experiment was selected to establish the optimal conditions. From these results, the filtration system for the investigation was assembled (Scheme 1). Therefore, the removal of methyl orange, methyl red and lead was carried out using the same filtration system established from the methylene blue results. Contaminated water is placed in a container, which has a submersible pump. This liquid is pumped through a regulating valve, which controls the water flow entering the filter. The filter consists of four layers, each containing 1.75 g of Sspp. The purified liquid is collected in another container. Every 10 min, this container was replaced by another and the volume of liquid removed at each time interval was collected for subsequent Ultraviolet-Visible (UV-Vis) analysis. In this way, 8 separate samples were obtained, since the duration of each experiment was 80 min. species S. natans and S. fluitans have a high adsorption capacity for the azo dyes, in particular for methylene blue, and for lead (II) cations. Sspp also requires a short time to reach equilibrium, suggesting that it could be used as a low-cost biosorbent in industrial-scale, efficient and economic wastewater treatments.

Filter System based on Sargassum spp. (Sspp)
To obtain the optimum filtration system, various parameters were evaluated, such as chemical treatment, grinding and the mass of the Sspp used, filter diameter, the number of layers, the flow rate of the solution and the degree of humidity of the Sspp. All these parameters were evaluated only with methylene blue. The best result obtained in each experiment was selected to establish the optimal conditions. From these results, the filtration system for the investigation was assembled (Scheme 1). Therefore, the removal of methyl orange, methyl red and lead was carried out using the same filtration system established from the methylene blue results. Contaminated water is placed in a container, which has a submersible pump. This liquid is pumped through a regulating valve, which controls the water flow entering the filter. The filter consists of four layers, each containing 1.75 g of Sspp. The purified liquid is collected in another container. Every 10 min, this container was replaced by another and the volume of liquid removed at each time interval was collected for subsequent Ultraviolet-Visible (UV-Vis) analysis. In this way, 8 separate samples were obtained, since the duration of each experiment was 80 min.

Materials
In this project, Sargassum ssp., collected from the Mexican Caribbean coast, were used. Sargassum spp. consists of a mixture of two different species: Sargassum fluitans ( Figure 1a) and Sargassum natans (Figure 1b). Methylene blue, methyl orange and methyl red, all reagent grade, were employed as dyes. The lead ion removal was evaluated using lead nitrate (Pb (NO3)2, American Chemical Society (ACS) reagent grade ≥ 99%). All reagents were purchased from Sigma-Aldrich. All solutions were prepared using deionized water.

Materials
In this project, Sargassum ssp., collected from the Mexican Caribbean coast, were used. Sargassum spp. consists of a mixture of two different species: Sargassum fluitans ( Figure 1a) and Sargassum natans (Figure 1b). Methylene blue, methyl orange and methyl red, all reagent grade, were employed as dyes. The lead ion removal was evaluated using lead nitrate (Pb (NO 3 ) 2 , American After collection, the Sspp was washed several times with tap water, and then with distilled water, to remove any litter and impurities. Then, it was dried in the sun for 48 h to remove all moisture. The Sspp was then subjected to chemical treatment with the aim of increasing the number of carboxylic groups. This was achieved through the oxidation of β glycosidic linkage, which reduces molecular weight and deproteinate and increases reducing sugar of the sample. This may increase the ability to chelate different toxic elements or substances [32]. For this process, 20 g of clean, dry Sspp was mixed with 1 L of a 10% solution of hydrogen peroxide (H2O2). The mixture was stirred magnetically for two hours. Finally, the chemically treated Sargassum sp. (CT-Sspp) was dried in an amber vacuum desiccator for 48 h and stored for later use. The characteristic molecular vibrational modes from the functional groups of Sspp and CT-Sspp were determined using Fourier Transform Infrared (FTIR) spectroscopy, using Perkin Elmer Spectrum Two equipment (Waltham, MA, United States), with attenuated total reflectance (ATR) in an interval from 600 to 4000 cm −1 . The Sspp was evaluated using a Hitachi SU8230 cold-field emission microscope operating at 3 keV. It should be noted that analyses were carried out before and after the samples were exposed to the contaminants.

Evaluation of Variables in the Filtration System
To optimize the elements of the filtration system, various parameters were examined. The amount of Sspp was varied to determine the ideal amount to remove the pollutants: amounts of 1, 5 and 7 g were evaluated. The effect of the surface area of Sspp was analyzed by decreasing the size of the Sspp particles, using crushed and ground Sspp. To increase the contact area between the Sspp and the water, the size of the filter was considered, diameters of 1.27 cm (0.5 inches), 2.54 cm (1 inch) and 3.81 cm (1.5 inches) were tested. The effect of residence time was examined, by regulating the flow of water, using three values (3, 5 and 9 mL/s). The distribution of the Sspp inside the filter was also evaluated, by varying the number of layers of the filter: 1, 2 and 4 layers. Finally, the removal rate in the early stages of the process was evaluated by varying the percentage of Sspp moisture (20%, 30% and 50%).

Analysis of the Samples Obtained by Biosorption
The samples obtained from the filtration were analyzed using UV-Vis spectroscopy, a fast and efficient technique, to determine the concentration of methylene blue, methyl orange, methyl red and Pb 2+ . In the UV-Vis spectrum, these organic dyes have a resonance effect because there is a change in energy levels in the Π-type bonds that are conjugated, also called the absorption band. The absorption bands of methylene blue, methyl orange and methyl red are situated at 664, 464 and 400 nm, After collection, the Sspp was washed several times with tap water, and then with distilled water, to remove any litter and impurities. Then, it was dried in the sun for 48 h to remove all moisture. The Sspp was then subjected to chemical treatment with the aim of increasing the number of carboxylic groups. This was achieved through the oxidation of β glycosidic linkage, which reduces molecular weight and deproteinate and increases reducing sugar of the sample. This may increase the ability to chelate different toxic elements or substances [32]. For this process, 20 g of clean, dry Sspp was mixed with 1 L of a 10% solution of hydrogen peroxide (H 2 O 2 ). The mixture was stirred magnetically for two hours. Finally, the chemically treated Sargassum sp. (CT-Sspp) was dried in an amber vacuum desiccator for 48 h and stored for later use. The characteristic molecular vibrational modes from the functional groups of Sspp and CT-Sspp were determined using Fourier Transform Infrared (FTIR) spectroscopy, using Perkin Elmer Spectrum Two equipment (Waltham, MA, United States), with attenuated total reflectance (ATR) in an interval from 600 to 4000 cm −1 . The Sspp was evaluated using a Hitachi SU8230 cold-field emission microscope operating at 3 keV. It should be noted that analyses were carried out before and after the samples were exposed to the contaminants.

Evaluation of Variables in the Filtration System
To optimize the elements of the filtration system, various parameters were examined. The amount of Sspp was varied to determine the ideal amount to remove the pollutants: amounts of 1, 5 and 7 g were evaluated. The effect of the surface area of Sspp was analyzed by decreasing the size of the Sspp particles, using crushed and ground Sspp. To increase the contact area between the Sspp and the water, the size of the filter was considered, diameters of 1.27 cm (0.5 inches), 2.54 cm (1 inch) and 3.81 cm (1.5 inches) were tested. The effect of residence time was examined, by regulating the flow of water, using three values (3, 5 and 9 mL/s). The distribution of the Sspp inside the filter was also evaluated, by varying the number of layers of the filter: 1, 2 and 4 layers. Finally, the removal rate in the early stages of the process was evaluated by varying the percentage of Sspp moisture (20%, 30% and 50%).

Analysis of the Samples Obtained by Biosorption
The samples obtained from the filtration were analyzed using UV-Vis spectroscopy, a fast and efficient technique, to determine the concentration of methylene blue, methyl orange, methyl red and Pb 2+ . In the UV-Vis spectrum, these organic dyes have a resonance effect because there is a change in energy levels in the Π-type bonds that are conjugated, also called the absorption band. The absorption bands of methylene blue, methyl orange and methyl red are situated at 664, 464 and 400 nm, respectively. As Pb 2+ does not have an absorption band, a complex based on 1,2-Diaminocyclohexane-N,N,Ní,Ní-tetraacetic acid (DACT) was synthesized and this showed an absorption band centered at 247 nm. The methodology to obtain this compound is described fully in Reference [33]. An initial Pb 2+ solution was prepared by dissolving nitric acid lead (II) nitrate in 500 mL of deionized H 2 O for a 100 mg*L −1 concentration; then, 10 mL of this solution was diluted up to 100 mL for an initial concentration of 10 mg*L −1 . Under this condition, the initial pH was 7.8. The calibration curves and the analysis of the samples obtained were performed by UV-Vis spectroscopy in a METASH 6000 M spectrometer. The analyses were performed using quartz cuvettes. The degree of concentration of each substance was made based on previously established calibration curves, using standards at various defined concentrations. The removal percentage of each substance was determined from the initial concentration and its concentration in each of the 8 samples, using the following equation: where C 0 is the initial concentration in parts per million (PPM) of the substance evaluated and C i is the concentration of the measured sample (PPM), obtained from the calibration curves. The results were plotted on removal percentage versus time graphs. The concentration measurements at the different time intervals were analyzed and fitted to a first order reaction, whose equation is described as follows: where k is the constant rate (s −1 ), t is the time (s) and A is an offset constant.

Characterization of Sargassum spp. by Scanning Electron Microscopy (SEM) and FTIR
Dry, clean Sargassum spp. (Sspp) was characterized by scanning electron microscopy (SEM) before and after the chemical treatment to observe its morphology, as shown in Figure 2. The physical processing caused disruption to the cells of the macroalgae, exposing the intracellular content. In Figure 2a, the pore structure of the Sspp is clearly observed. The structured arrangement of the interconnected tube cells is visible, and the cell wall of the intercellular matrix remains almost intact. However, in Figure 2b, it can be seen that the surface morphology of the sample was significantly changed, due to the impregnation of hydrogen peroxide (H 2 O 2 ). The pore structure on the surface of the sample is partially destroyed, probably caused by strong cross-linking between the OH+ and negatively charged chemical groups in the cells. The surface is now rough and irregular. respectively. As Pb 2+ does not have an absorption band, a complex based on 1,2-Diaminocyclohexane-N,N,Ní,Ní-tetraacetic acid (DACT) was synthesized and this showed an absorption band centered at 247 nm. The methodology to obtain this compound is described fully in Reference [33]. An initial Pb 2+ solution was prepared by dissolving nitric acid lead (II) nitrate in 500 mL of deionized H2O for a 100 mg*L −1 concentration; then, 10 mL of this solution was diluted up to 100 mL for an initial concentration of 10 mg*L −1 . Under this condition, the initial pH was 7.8. The calibration curves and the analysis of the samples obtained were performed by UV-Vis spectroscopy in a METASH 6000 M spectrometer. The analyses were performed using quartz cuvettes. The degree of concentration of each substance was made based on previously established calibration curves, using standards at various defined concentrations. The removal percentage of each substance was determined from the initial concentration and its concentration in each of the 8 samples, using the following equation: where C0 is the initial concentration in parts per million (PPM) of the substance evaluated and Ci is the concentration of the measured sample (PPM), obtained from the calibration curves. The results were plotted on removal percentage versus time graphs. The concentration measurements at the different time intervals were analyzed and fitted to a first order reaction, whose equation is described as follows:

= +
where k is the constant rate (s −1 ), t is the time (s) and A is an offset constant.

Characterization of Sargassum spp. by Scanning Electron Microscopy (SEM) and FTIR
Dry, clean Sargassum spp. (Sspp) was characterized by scanning electron microscopy (SEM) before and after the chemical treatment to observe its morphology, as shown in Figure 2. The physical processing caused disruption to the cells of the macroalgae, exposing the intracellular content. In Figure 2a, the pore structure of the Sspp is clearly observed. The structured arrangement of the interconnected tube cells is visible, and the cell wall of the intercellular matrix remains almost intact. However, in Figure 2b, it can be seen that the surface morphology of the sample was significantly changed, due to the impregnation of hydrogen peroxide (H2O2). The pore structure on the surface of the sample is partially destroyed, probably caused by strong cross-linking between the OH+ and negatively charged chemical groups in the cells. The surface is now rough and irregular.    The performed vibrational assignment indicates that the main macromolecular structure is not damaged by the chemical treatment. The characteristic bands of the polysaccharide groups present in their structure [32] are located at 3000-3500 cm −1 from OH symmetric stretching, at 1410 cm −1 from a combination of C-O and O-H deformation vibrations and finally, the bands present at 1219 cm −1 and 1023 cm −1 are both related to C-O stretching vibration. However, differences were found between the dry and CT-Sspp samples due to the effect of the H 2 O 2 in the chemical treatment. In the dry Sspp, two peaks were found at 3365 cm −1 and 3274 cm −1 , corresponding to N-H and O-H stretch vibration respectively, while in the CT-Sspp, there is only one peak assigned to an O-H symmetric stretching vibration. The disappearance of the N-H stretching vibration has been observed in other polysaccharides, such as chitosan, suggesting that terminal NH 2 groups are removed from chains during the chemical treatment due to the deamination side reactions [34]. Dry Sspp had two peaks at 2977 and 2926 cm −1 for symmetric and asymmetric C-H vibrations, while in the CT-Sspp, only the peak from symmetric stretching (2926 cm −1 ) was seen. by the chemical treatment. The characteristic bands of the polysaccharide groups present in their structure [32] are located at 3000-3500 cm −1 from OH symmetric stretching, at 1410 cm −1 from a combination of C-O and O-H deformation vibrations and finally, the bands present at 1219 cm −1 and 1023 cm −1 are both related to C-O stretching vibration. However, differences were found between the dry and CT-Sspp samples due to the effect of the H2O2 in the chemical treatment. In the dry Sspp, two peaks were found at 3365 cm −1 and 3274 cm −1 , corresponding to N-H and O-H stretch vibration respectively, while in the CT-Sspp, there is only one peak assigned to an O-H symmetric stretching vibration. The disappearance of the N-H stretching vibration has been observed in other polysaccharides, such as chitosan, suggesting that terminal NH2 groups are removed from chains during the chemical treatment due to the deamination side reactions [34]. Dry Sspp had two peaks at 2977 and 2926 cm −1 for symmetric and asymmetric C-H vibrations, while in the CT-Sspp, only the peak from symmetric stretching (2926 cm −1 ) was seen. On the other hand, a shift from 1601 cm −1 to 1615 cm −1 was found for treated and dry Sspp, indicating a modification in the C=O vibrational state from a change in the polysaccharide structure from the cyclic to the non-cyclic form, as part of the structural changes from the chemical treatment. The modifications on the signals corresponding to the -OH vibration could be related to an increasing number of carboxyl groups, due to the oxidation cleavage of the β-glycosidic groups, which is confirmed with an increase on the C-O band at 1219 cm −1 and a decrease in the band of the glycosidic linkage vibration present at 873 cm −1 .
These results agree with works of other authors. For example, Sargassum boveanum has been reported [35] in the region of 400 to 4000 cm −1 , in which bands related to -OH broad stretch for high concentration of alcohols or phenols are found. Also, bands located from C-O stretching from C=O and C-C stretching from phenol rings were found. Another example of concordance with the proposed vibrational assignment is the report from Sargassum wightii [36], in which bands of about 600-800 cm −1 and bands around 1258 cm −1 related to S=O and C-C-O vibration from lignin were presented. Also, bands located at 3371 and 3408 cm −1 corresponding to N-H and O-H stretching of the polysaccharides were observed. This indicates that the Sargassum sp. studied in the present work 4000 3500 3000 2500 2000 1500 1000 Transmittance (a.u.) On the other hand, a shift from 1601 cm −1 to 1615 cm −1 was found for treated and dry Sspp, indicating a modification in the C=O vibrational state from a change in the polysaccharide structure from the cyclic to the non-cyclic form, as part of the structural changes from the chemical treatment. The modifications on the signals corresponding to the -OH vibration could be related to an increasing number of carboxyl groups, due to the oxidation cleavage of the β-glycosidic groups, which is confirmed with an increase on the C-O band at 1219 cm −1 and a decrease in the band of the glycosidic linkage vibration present at 873 cm −1 .
These results agree with works of other authors. For example, Sargassum boveanum has been reported [35] in the region of 400 to 4000 cm −1 , in which bands related to -OH broad stretch for high concentration of alcohols or phenols are found. Also, bands located from C-O stretching from C=O and C-C stretching from phenol rings were found. Another example of concordance with the proposed vibrational assignment is the report from Sargassum wightii [36], in which bands of about 600-800 cm −1 and bands around 1258 cm −1 related to S=O and C-C-O vibration from lignin were presented. Also, bands located at 3371 and 3408 cm −1 corresponding to N-H and O-H stretching of the polysaccharides were observed. This indicates that the Sargassum sp. studied in the present work has low quantities of sulfonated polysaccharides. Moreover, as previously mentioned, the amino groups were removed, and an increasing number of carboxylic groups was formed from deamination and from molecular structure degradation from the oxidized cleavage of β-glycosidic linkages. Thus, the structure of the residue and the reducing end has been modified [35]. In summary, the chemical treatment caused changes in the chemical structure and there were more functional groups available for the adsorption of toxic substances.

Effect of Chemical Treatment of Sargassum spp.
As has been reported, H 2 O 2 modifies the structural chains of the biomass, leading to an increase in the absorption of the substances evaluated [32,34]. The degree of removal of methylene blue was analyzed. Figure 4 shows the evaluation of chemically treated Sspp in methylene blue removal. Figure 4a,b shows the UV-Vis spectra, taken every 10 min, for dry Sspp and CT-Sspp, respectively. There is a decrease in the intensity of the methylene blue absorption band, meaning a decrease in the concentration of the dye. The decrease in the spectra intensity is less for dry Sspp than for CT-Sspp. Figure 4c shows the removal rate curves obtained from the UV-Vis spectra data. The removal of methylene blue, using non-treated Sspp, was approximately 80%, while with CT-Sspp, the removal was close to 95%. However, in both cases, these removal rates were reached very slowly, after 60 min. Finally, the removal rate was also notably higher in the case of chemically modified Sspp (k = 0.131 s −1 ) compared to non-modified Sspp (k = 0.129 s −1 ). Therefore, the chemical treatment of Sspp improves the rate and efficiency of methylene blue removal. has low quantities of sulfonated polysaccharides. Moreover, as previously mentioned, the amino groups were removed, and an increasing number of carboxylic groups was formed from deamination and from molecular structure degradation from the oxidized cleavage of β-glycosidic linkages. Thus, the structure of the residue and the reducing end has been modified [35]. In summary, the chemical treatment caused changes in the chemical structure and there were more functional groups available for the adsorption of toxic substances.

Effect of Chemical Treatment of Sargassum spp.
As has been reported, H2O2 modifies the structural chains of the biomass, leading to an increase in the absorption of the substances evaluated [32,34]. The degree of removal of methylene blue was analyzed. Figure 4 shows the evaluation of chemically treated Sspp in methylene blue removal. Figure 4a,b shows the UV-Vis spectra, taken every 10 min, for dry Sspp and CT-Sspp, respectively. There is a decrease in the intensity of the methylene blue absorption band, meaning a decrease in the concentration of the dye. The decrease in the spectra intensity is less for dry Sspp than for CT-Sspp. Figure 4c shows the removal rate curves obtained from the UV-Vis spectra data. The removal of methylene blue, using non-treated Sspp, was approximately 80%, while with CT-Sspp, the removal was close to 95%. However, in both cases, these removal rates were reached very slowly, after 60 min. Finally, the removal rate was also notably higher in the case of chemically modified Sspp (k = 0.131 s −1 ) compared to non-modified Sspp (k = 0.129 s −1 ). Therefore, the chemical treatment of Sspp improves the rate and efficiency of methylene blue removal.

Amount of Sargassum spp. in Filters
Once it was demonstrated that Sspp could remove methylene blue, the amount of CT-Sspp used in the filters was increased, in order to improve the rate and the amount of removal. Figure 5 shows the results of methylene blue removal, with varying masses of CT-Sspp: 1, 3 and 7 g. The samples

Amount of Sargassum spp. in Filters
Once it was demonstrated that Sspp could remove methylene blue, the amount of CT-Sspp used in the filters was increased, in order to improve the rate and the amount of removal. Figure 5 shows the results of methylene blue removal, with varying masses of CT-Sspp: 1, 3 and 7 g. The samples were taken every 10 min and characterized by UV-Vis (Figure 5a, b and c, respectively). As observed, the absorption band of methylene blue decreases with time, indicating its removal. Figure 5d shows the removal curves obtained from the spectra, where both the rate and the removal efficiency can be determined. As can be seen, the efficiency of the three conditions evaluated is very similar, around 90%. However, it should be noted that in the case of 7 g of CT-Sspp, the removal rate was considerably higher. This data can be corroborated by comparing the rate constants obtained in each curve. The values of this constant were 0.06, 0.082 and 0.163 s −1 for 1, 3 and 7 g of CT-Sspp, respectively. With 7 g of CT-Sspp, a high level of methylene blue removal was achieved in a short time.

Mechanically Treated Sargassum spp.
To increase the exposed surface area of the CT-Sspp, two mechanical processes were applied to it: crushing and grinding. Figure 6 shows the results of methylene blue removal using 7 g CT-Sspp subjected to these mechanical treatments. Figure 6a,b shows the UV-Vis spectra recorded every 10 min, when crushed and ground CT-Sspp was used, respectively. In both cases, a rapid decrease in the absorption band of the dye is appreciated. However, after a short time, removal became constant and complete removal did not occur. The outflow gradually narrowed, until a plug was produced, preventing the flow of water. This was due to the rapid and significant compaction of the CT-Sspp because of the reduction in particle size caused by the mechanical treatment. Figure 6c shows the curves obtained from the UV-Vis spectra. In the first stages, the curves are very similar; later, a slightly better performance for the crushed CT-Sspp is seen. However, no improvement in total removal was obtained with respect to experiments without mechanical treatment, so it was decided to use CT-Sspp without mechanical treatment in the experiments.

Mechanically Treated Sargassum spp.
To increase the exposed surface area of the CT-Sspp, two mechanical processes were applied to it: crushing and grinding. Figure 6 shows the results of methylene blue removal using 7 g CT-Sspp subjected to these mechanical treatments. Figure 6a,b shows the UV-Vis spectra recorded every 10 min, when crushed and ground CT-Sspp was used, respectively. In both cases, a rapid decrease in the absorption band of the dye is appreciated. However, after a short time, removal became constant and complete removal did not occur. The outflow gradually narrowed, until a plug was produced, preventing the flow of water. This was due to the rapid and significant compaction of the CT-Sspp because of the reduction in particle size caused by the mechanical treatment. Figure 6c shows the curves obtained from the UV-Vis spectra. In the first stages, the curves are very similar; later, a slightly better performance for the crushed CT-Sspp is seen. However, no improvement in total removal was obtained with respect to experiments without mechanical treatment, so it was decided to use CT-Sspp without mechanical treatment in the experiments.   (Figure 7a), the absorption band of the dye decreases rapidly, at first. However, after a short time, the spectra show no significant changes. Figure 7b, corresponding to a filter of 2.54 cm of diameter, shows a constant decrease in the absorption band of methylene blue. When the diameter was increased to 3.81 cm (Figure 7c), a rapid decrease in the absorption band was observed in the first spectrum. However, afterwards, the spectra recorded only a very slight decrease. These results are most noticeable in the removal curves shown in Figure 7d. The removal percentage in the first 10 min is very similar in the three curves, but, as time advances, the removal is more efficient with a filter of 2.54 cm, reaching 95% removal. Therefore, a filter of 2.54 cm (1 inch) diameter was selected for the subsequent experiments.   (Figure 7a), the absorption band of the dye decreases rapidly, at first. However, after a short time, the spectra show no significant changes. Figure 7b, corresponding to a filter of 2.54 cm of diameter, shows a constant decrease in the absorption band of methylene blue. When the diameter was increased to 3.81 cm (Figure 7c), a rapid decrease in the absorption band was observed in the first spectrum. However, afterwards, the spectra recorded only a very slight decrease. These results are most noticeable in the removal curves shown in Figure 7d. The removal percentage in the first 10 min is very similar in the three curves, but, as time advances, the removal is more efficient with a filter of 2.54 cm, reaching 95% removal. Therefore, a filter of 2.54 cm (1 inch) diameter was selected for the subsequent experiments.

Evaluation of Filter Layers
In order to increase the percentage and rate of methylene blue removal, the amount of CT-Sspp (7 g) was divided into various numbers of layers. The results are shown in Figure 8. The UV-Vis spectra of methylene blue using C1 = 2, C2 = 3 and C3 = 4 layers of CT-Sspp are shown in Figure 8a, b and c, respectively. In all cases, the behavior is similar, that is, the signal or absorption band, corresponding to the dye, decreases rapidly in the first spectrum until it is no longer appreciated in the last measurements. Figure 8d shows the removal curves where the evolution of each condition can be seen in greater detail. Comparing the three curves, it can be seen that when 4 layers were used, the greatest efficiency was observed from the first measurement, 80%. Total removal was very similar in the three experiments, of approximately 98%. Regarding the rate constants, values of 0.124, 0.139 and 0.161 s −1 were obtained for 2, 3 and 4 layers, respectively. As can be observed, the removal rates for the entire experiment are very similar. However, a better dye removal should be noted in the early stages when the Sargassum is distributed in 4 layers. Therefore, the use of 4 layers gives the highest rate of methylene blue removal. These results show a notable improvement with respect to those that had been obtained so far. Therefore, for subsequent experiments, the system had four layers of CT-Sspp, equally divided.

Evaluation of Filter Layers
In order to increase the percentage and rate of methylene blue removal, the amount of CT-Sspp (7 g) was divided into various numbers of layers. The results are shown in Figure 8. The UV-Vis spectra of methylene blue using C1 = 2, C2 = 3 and C3 = 4 layers of CT-Sspp are shown in Figure 8a, b and c, respectively. In all cases, the behavior is similar, that is, the signal or absorption band, corresponding to the dye, decreases rapidly in the first spectrum until it is no longer appreciated in the last measurements. Figure 8d shows the removal curves where the evolution of each condition can be seen in greater detail. Comparing the three curves, it can be seen that when 4 layers were used, the greatest efficiency was observed from the first measurement, 80%. Total removal was very similar in the three experiments, of approximately 98%. Regarding the rate constants, values of 0.124, 0.139 and 0.161 s −1 were obtained for 2, 3 and 4 layers, respectively. As can be observed, the removal rates for the entire experiment are very similar. However, a better dye removal should be noted in the early stages when the Sargassum is distributed in 4 layers. Therefore, the use of 4 layers gives the highest rate of methylene blue removal. These results show a notable improvement with respect to those that had been obtained so far. Therefore, for subsequent experiments, the system had four layers of CT-Sspp, equally divided.

Evaluation of Water Flow Rate
By regulating the flow, the residence time of the fluid in the filter is altered, which may result in an increase in the removal efficiency. Figure 9 shows the results obtained by varying the fluid flow to the filter inlet. Figure 9a, b and c shows the UV-Vis spectra of methylene blue for flows of 3 (F1), 5 (F2) and 9 mL/s (F3), respectively. In all cases, the absorption band of methylene blue decreases rapidly. This happens faster as the flow is decreased. Thus, when the flow was 3 mL/s, the first spectrum did not show the dye absorption band, suggesting a high removal. Figure 9d shows the removal curves for the three flows. The total removal of the three evaluated flows are similar. The flows of 5 and 9 mL/s show similar behavior, with the first flow being slightly more efficient. When the flow was 3 mL/s, it is observed that in the first measurement, a removal of approximately 87% was obtained. After 30 min, 98% methylene blue removal was achieved. After this time, removal continued slowly, reaching an efficiency close to 100%. This flow rate gave the best result, as it increases the contact time of the CT-Sspp and the fluid, thereby significantly increasing the removal rate of methylene blue.

Evaluation of Water Flow Rate
By regulating the flow, the residence time of the fluid in the filter is altered, which may result in an increase in the removal efficiency. Figure 9 shows the results obtained by varying the fluid flow to the filter inlet. Figure 9a, b and c shows the UV-Vis spectra of methylene blue for flows of 3 (F1), 5 (F2) and 9 mL/s (F3), respectively. In all cases, the absorption band of methylene blue decreases rapidly. This happens faster as the flow is decreased. Thus, when the flow was 3 mL/s, the first spectrum did not show the dye absorption band, suggesting a high removal. Figure 9d shows the removal curves for the three flows. The total removal of the three evaluated flows are similar. The flows of 5 and 9 mL/s show similar behavior, with the first flow being slightly more efficient. When the flow was 3 mL/s, it is observed that in the first measurement, a removal of approximately 87% was obtained. After 30 min, 98% methylene blue removal was achieved. After this time, removal continued slowly, reaching an efficiency close to 100%. This flow rate gave the best result, as it increases the contact time of the CT-Sspp and the fluid, thereby significantly increasing the removal rate of methylene blue.

Evaluation of Sargassum spp. Humidity in the Filters
The potential of CT-Sspp to remove contaminants such as methylene blue is demonstrated above. However, maximum removal is obtained after 20 to 30 min, meaning that in the first 30 min, the water still contains a considerable amount of the dye. Therefore, improving the efficiency of the system in the initial phase would be beneficial. Detailed analysis shows that the time of residence

Evaluation of Sargassum spp. Humidity in the Filters
The potential of CT-Sspp to remove contaminants such as methylene blue is demonstrated above. However, maximum removal is obtained after 20 to 30 min, meaning that in the first 30 min, the water still contains a considerable amount of the dye. Therefore, improving the efficiency of the system in the initial phase would be beneficial. Detailed analysis shows that the time of residence inside the filter was very short. As the experiment proceeds, the CT-Sspp expands as it absorbs the liquid, leading to an increase in residence time. In some cases, the outlet duct actually became plugged. Therefore, the CT-Sspp was moistened before being placed in the filter. Figure 10 shows the results for the different humidity values of the CT-Sspp in dye removal. Figure 10a, b and c shows the UV-Vis spectra for humidity of H1 = 20 wt%, H2 = 30 wt% and H3 = 50 wt%, respectively. In all three cases, the absorption band of methylene blue disappears after 10 min and remains unchanged until 80 min. Figure 10d shows the removal curves for the three cases, showing similar patterns to those seen in the spectra. The first measurement, after 10 min, sees a 98% removal, increasing to slightly more than 99%. As there was no significant difference between the three humidity values, it was decided to moisten the CT-Sspp at only 20 wt%. In this way, a high degree/rate/amount of methylene blue was removed from the earliest stages of the process.

Removal of Methyl Orange and Methyl Red
Once the ideal parameters and conditions were established for methylene blue removal, methyl orange and methyl red removal was considered. The results of both are shown in Figure 11. Figure  11a shows the methyl red UV-Vis spectra recorded every 10 min. This dye has an absorption band at 400 nm. There is a significant decrease in the intensity of the absorption band after the first sample, which means that a significant amount of the dye was removed by the CT-Sspp. However, in subsequent samples, there are no significant changes to the spectra, suggesting that dye removal had stopped. This may be due to saturation in the removal capacity of the CT-Sspp. Figure 11b shows the UV-Vis spectra for methyl orange samples. In this case, the absorption band of this dye is at 465 nm. In the first samples, the absorption band decreases slowly over time. In the final samples, there are insignificant changes in the spectra, indicating a halt in the dye removal process. Figure 11c shows the removal curves for methyl red and methyl orange obtained from the UV-Vis data. In the case of

Removal of Methyl Orange and Methyl Red
Once the ideal parameters and conditions were established for methylene blue removal, methyl orange and methyl red removal was considered. The results of both are shown in Figure 11. Figure 11a shows the methyl red UV-Vis spectra recorded every 10 min. This dye has an absorption band at 400 nm. There is a significant decrease in the intensity of the absorption band after the first sample, which means that a significant amount of the dye was removed by the CT-Sspp. However, in subsequent samples, there are no significant changes to the spectra, suggesting that dye removal had stopped. This may be due to saturation in the removal capacity of the CT-Sspp. Figure 11b shows the UV-Vis spectra for methyl orange samples. In this case, the absorption band of this dye is at 465 nm. In the first samples, the absorption band decreases slowly over time. In the final samples, there are insignificant changes in the spectra, indicating a halt in the dye removal process. Figure 11c shows the removal curves for methyl red and methyl orange obtained from the UV-Vis data. In the case of methyl red, a 60% removal is observed in the first 10 min. Subsequently, only small changes are observed, reaching a total removal of approximately 68%. As regards methyl orange, a slow absorption is observed for the first 30 min. Subsequently, no significant changes were observed, achieving only 25% removal. The rate constants were 0.222 and 0.15 s −1 for methyl red and orange, respectively. These results are in agreement with the observations of the UV-Vis spectra and demonstrate the ability of CT-Sspp to remove this kind of dye. However, a complete removal of methyl red and methyl orange is not possible as they are azo dyes and early saturation of the biosorbent may occur [37]. Although complete elimination of methyl orange was not achieved (25%), the results are important, since this dye is difficult to eliminate from aqueous solutions due to its high solubility. The same percentage of methylene dye removal (25%) is reported elsewhere [38], after 48 and 24 h, using pure alginate and alginate combined with TiO 2 photocatalyst, respectively. Maximum elimination (40% and 100%, respectively) was achieved after 96 h. On the other hand, Tabaraki and Sadeghinejad [39] reported the removal of 13.25% of this dye, using Sargassum ilicifolium as biosorbent. Therefore, the filtration system described in this paper is seen to be simple, fast, efficient and competitive for a persistently high removal of these dyes. For methyl red, there are no reports using similar removal methods.  In Figure 12, the FTIR spectra of the CT-Sspp is compared with its spectra after the removal of the dyes studied. Five different zones are marked to make accurate comparisons of the observed chemical structure changes in the functional groups' vibrations. In the first zone, a major change in the hydroxyl band is seen at 3300 cm −1 , that is assigned to O-H symmetric vibration, since the intensity is reduced, and the band is wider. This indicates that hydrogen bonding between any of In Figure 12, the FTIR spectra of the CT-Sspp is compared with its spectra after the removal of the dyes studied. Five different zones are marked to make accurate comparisons of the observed chemical structure changes in the functional groups' vibrations. In the first zone, a major change in the hydroxyl band is seen at 3300 cm −1 , that is assigned to O-H symmetric vibration, since the intensity is reduced, and the band is wider. This indicates that hydrogen bonding between any of these dyes and CT-Sspp is the principal mechanism for the bio-absorption recorded. There is also a change in the intensity of the band present at 1615 cm −1 in zone II (its intensity is reduced). This indicates a contribution in the dye uptake by charge interaction from a donor-accepting group, e.g., the possible interaction between the partially negative charge in the carboxyl groups from Sspp and the positive charge from amine/sulfate/carboxylic groups present in the dyes. This interaction is confirmed by observing the changes in the bands in zone III and zone IV. In zone IV, it is observed that the band assigned to C-OH located at 1023 cm −1 decreased, since the carboxylic group is now negatively charged. In zone III, there is an increase in the assigned stretching absorption band at 1410 cm −1 from the combined interaction of C-O and O-H, indicating that the interaction from the carbonyl group is higher. Additional confirmation for hydrogen bonding is seen in zone V, where the band at 872 cm −1 assigned to an OH-O deformation out of plane increased from this kind of interaction. With the charge interaction and hydrogen bonding mechanism that has been previously proposed [31], a scheme of how the dyes can interact with the polysaccharide structure of CT-Sspp is shown in Scheme 2. SEM images were obtained to understand the impact of the dyes on the CT-Sspp. The changes in Sspp morphology are generally due to the breaking of the cross-links between the dyes and the negatively charged chemical groups of the cell wall. Figure 13a shows the SEM image of the CT-Sspp sample loaded with methylene blue, where the methylene blue molecules attach to the CT-Sspp surface and fill the pores. Figure 13b shows the CT-Sspp sample loaded with methyl orange, where the surface became rough with numerous agglomerates. Some methyl orange crystals were even observed. The surface of the sample is similar in appearance to the sample before loading. However, this surface change indicates that methyl orange molecules were sorbed by the CT-Sspp. After sorption, the methyl red molecules remain as aggregates on the CT-Sspp surface (Figure 13c). Like the CT-Sspp sample with methyl orange, the surface shows similar characteristics to a sample before loading with methyl red. SEM images were obtained to understand the impact of the dyes on the CT-Sspp. The changes in Sspp morphology are generally due to the breaking of the cross-links between the dyes and the negatively charged chemical groups of the cell wall. Figure 13a shows the SEM image of the CT-Sspp sample loaded with methylene blue, where the methylene blue molecules attach to the CT-Sspp surface and fill the pores. Figure 13b shows the CT-Sspp sample loaded with methyl orange, where the surface became rough with numerous agglomerates. Some methyl orange crystals were even observed. The surface of the sample is similar in appearance to the sample before loading. However, this surface change indicates that methyl orange molecules were sorbed by the CT-Sspp. After sorption, the methyl red molecules remain as aggregates on the CT-Sspp surface (Figure 13c). Like the CT-Sspp sample with methyl orange, the surface shows similar characteristics to a sample before loading with methyl red. The physicochemical parameters and the removal percentages obtained in the removal of the dyes are summarized in Table 1. It can be seen that the efficiency and/or the rate of methylene blue removal was improved with the parameters implemented, up to a value of almost 100%, in a short period of time. As regards methyl orange and methyl red, only one experiment was carried out, using those parameters giving the best results for methylene blue.  The physicochemical parameters and the removal percentages obtained in the removal of the dyes are summarized in Table 1. It can be seen that the efficiency and/or the rate of methylene blue removal was improved with the parameters implemented, up to a value of almost 100%, in a short period of time. As regards methyl orange and methyl red, only one experiment was carried out, using those parameters giving the best results for methylene blue. Figure 14 shows the results obtained for the removal of Pb ions. In Figure 14a, the lead compound is shown on the UV-Vis spectra as a band at 245 nm. In the first spectrum, there was a rapid decrease in the intensity of the band. Subsequently, the decrease in band intensity continues at a slower rate. After 80 min, the spectra did not show significant changes. Figure 14b shows the removal curves obtained from the UV-Vis spectra. The removal percentage after 40 min is close to 90%. Lead removal continues, reaching 95% after 80 min. The rate constant obtained from this curve was 0.068 s −1 . These results demonstrate the ability of CT-Sspp to remove metals such as lead.  Figure 15a shows the FTIR analysis of the CT-Sspp after lead (II) ions removal. Zones of interest are marked by dashed rectangles. Significant changes are seen, specifically in zone I, zone II and zone IV. First, the change in zone I is from the O-H symmetric stretching band, which is augmented and slightly sharper. This is because of the interaction between the CT-Sspp and lead (II), in which chelation is now the main process [40]. This is confirmed when zone II to zone V are analyzed: the interactions from diverse carbonyl vibration modes are being affected by chelation. In zone II, the intensity of the C=O band at 1615 cm −1 is increased, indicating a change from a donor-accepting group (e.g., a proton from Pb 2+ ). This effect is also accompanied by an increase on the stretching band of C-O from zone II, at 1410 cm −1 . An increase on the asymmetric stretching vibration band from C-O, at 1219 cm −1 , and an increase from the band located at 1023 cm −1 assigned to C-OH stretch from the polysaccharide cyclic structure in the zones III and IV respectively, is also observed. All changes seen in the vibrational modes are related to the electrostatic interaction. Finally, in zone VI, the band at 672 cm −1 , which comes from the interaction between the organic molecule and lead, is wider and has higher intensity. This type of interaction has been seen in the synthesis of lead nanoparticles [41], and is related to Pb-O asymmetric bending vibration (Figure 15b). Figure 14 shows the results obtained for the removal of Pb ions. In Figure 14a, the lead compound is shown on the UV-Vis spectra as a band at 245 nm. In the first spectrum, there was a rapid decrease in the intensity of the band. Subsequently, the decrease in band intensity continues at a slower rate. After 80 min, the spectra did not show significant changes. Figure 14b shows the removal curves obtained from the UV-Vis spectra. The removal percentage after 40 min is close to 90%. Lead removal continues, reaching 95% after 80 min. The rate constant obtained from this curve was 0.068 s −1 . These results demonstrate the ability of CT-Sspp to remove metals such as lead.  IV. First, the change in zone I is from the O-H symmetric stretching band, which is augmented and slightly sharper. This is because of the interaction between the CT-Sspp and lead (II), in which chelation is now the main process [40]. This is confirmed when zone II to zone V are analyzed: the interactions from diverse carbonyl vibration modes are being affected by chelation. In zone II, the intensity of the C=O band at 1615 cm −1 is increased, indicating a change from a donor-accepting group (e.g., a proton from Pb 2+ ). This effect is also accompanied by an increase on the stretching band of C-O from zone II, at 1410 cm −1 . An increase on the asymmetric stretching vibration band from C-O, at 1219 cm −1 , and an increase from the band located at 1023 cm −1 assigned to C-OH stretch from the polysaccharide cyclic structure in the zones III and IV respectively, is also observed. All changes seen in the vibrational modes are related to the electrostatic interaction. Finally, in zone VI, the band at 672 cm −1 , which comes from the interaction between the organic molecule and lead, is wider and has higher intensity. This type of interaction has been seen in the synthesis of lead nanoparticles [41], and is related to Pb-O asymmetric bending vibration (Figure 15b). Figure 15c shows a SEM image of CT-Sspp after lead removal. When the CT-Sspp samples were exposed to the metal solution (Pb), the structure continuity and the free area between the intracellular spaces were broken. In addition, morphological changes were clearly seen in the cell wall matrix, such as shrinking, sticking of layers and a tendency for the cell wall to be deformed. Similar changes in the cell wall matrix of different Sargassum spp. biomass when they were enriched with ions via biosorption have been observed by other authors [42,43]. 3.11. Comparison of Substance Removal using Sargassum spp. Table 2 shows a comparison of the results obtained in this work with those reported elsewhere. The species of Sargassum used and the removal percentages are included. As can be seen in Table 2, all the previous works concern static systems, and to the best of the authors' knowledge, this is the  Figure 15c shows a SEM image of CT-Sspp after lead removal. When the CT-Sspp samples were exposed to the metal solution (Pb), the structure continuity and the free area between the intracellular spaces were broken. In addition, morphological changes were clearly seen in the cell wall matrix, such as shrinking, sticking of layers and a tendency for the cell wall to be deformed. Similar changes in the cell wall matrix of different Sargassum spp. biomass when they were enriched with ions via biosorption have been observed by other authors [42,43].

Removal of Pb Ions
3.11. Comparison of Substance Removal using Sargassum spp. Table 2 shows a comparison of the results obtained in this work with those reported elsewhere. The species of Sargassum used and the removal percentages are included. As can be seen in Table 2, all the previous works concern static systems, and to the best of the authors' knowledge, this is the first study on this kind of dynamic system, where a scalable filter is proposed as a device for the removal of toxic substances. It is therefore difficult to compare the results of the various other works. An increase in the contact area of the filter produces a later breakthrough time. This produces greater uptake capacity, since the pollutant has a higher residence time with the adsorbate and has sufficient time to diffuse to the active moieties, responsible for the removal process. On the other hand, many other factors may influence the removal capacity, for example, composition, functionalization and modification treatments of Sargassum spp.

Sargassum
Species filter became clogged due to the absorption of water by the CT-Sargassum spp. Increasing the mass of CT-Sargassum spp. used in the filter gave greater removal at a faster rate. The diameter of the filter used and the water flow were also evaluated. In the first case, the highest removal was obtained when a 1 inch diameter filter was used. In the second case, it was observed that as the flow decreases, the removal rate and efficiency increase: a flow of 3 mL/s was found to be the optimum rate of flow. With respect to the number of layers (plates), as expected, removal was greater with an increased number of adsorption layers. In this work, a maximum of 4 layers were used. The experiments performed show that the Sargassum spp.-based water filter system was efficient. The efficiency and the rate of removal were different for each substance. The total removal of methylene blue, methyl red and methyl orange was 99%, 68% and 25%, respectively. Regarding lead ions, the removal percentage obtained was 95%. These results demonstrate the ability of CT-Sargassum spp. to trap and remove specific water-soluble toxic substances from water. This kind of dynamic biosorbent filter system is therefore an eco-friendly, scalable and cost-effective option for domestic, agricultural and industrial purposes. The future work of the present study is to evaluate the removal of pollutants using real samples taken from effluents of rivers, lakes or seas, which contain various pollutants at different concentrations.