Beachrock Formation Mechanism Using Multiproxy Experimental Data from Natural and Artiﬁcial Beachrocks: Insights for a Potential Soft Engineering Method

: Beachrocks are a window to the past environmental, geological, sedimentological and morphological conditions that were dominant in the coastal zone during their formation. Further-more, beachrocks have the ability to reduce coastal erosion impact on sandy beaches. This study focuses on the beachrock formation mechanism through the comparison of cement characteristics, mineral chemistry and sedimentology of beachrock occurrences from two different geological and geographical localities: Diolkos, Corinth, Greece and Sumuide, Okinawa, Japan. In addition, in order to investigate a potential soft engineering method to protect coasts from erosion, artiﬁcial beachrock samples were created in vitro using sand samples and ureolytic bacteria from both areas under accelerating conditions. For Okinawa artiﬁcial beachrock experiments, the bacteria Pararhodobacter sp. was used, and for Diolkos, it was the bacteria Micrococcus yunnainensis sp. For the natural beachrocks, a multi-analytical approach was accomplished with the use of microscopic investigation, a scanning electron microscope, energy-dispersive X-ray spectroscopy, X-ray diffraction and X-ray ﬂuorescence. Correlations were made between natural and artiﬁcial beachrocks. Results have shown that Diolkos beachrock was formed in the upper part of the intertidal zone, consisting of detrital material originating from the local bedrock, while Sumuide beachrock formed in the low intertidal–upper subtidal zone, consisting of coral sand and foraminifera fragments. For the artiﬁcial beachrocks, three samples were created using the microbial-induced carbonate precipitation (MICP) method, one from Diolkos (Corinth, Greece) and two from Sumuide (Okinawa, Japan). Diolkos artiﬁcial beachrock was better consolidated in comparison to Sumuide. Our investigation has shown that bacterial density was the key factor for the creation of the artiﬁcial beachrocks, while the samples’ granulometry played a secondary role in the process. The laboratory artiﬁcial beachrocks show encouraging results for a new soft engineering method to encounter beach erosion while keeping an ecofriendly character by saving energy, material resources and gas emissions. Artiﬁcial beachrocks can share the same properties of a natural beachrock and can contribute positively to marine biodiversity as a natural rocky habitat. Microanalyses were performed on epoxy resin-impregnated polished and carbon-coated thin sections. The measured concentrations were automatically corrected for atomic number, absorption in the sample, ﬂuorescence and dead time (the correction) using the The chemical composition of the minerals was determined using natural minerals and synthetic oxide standards, and 20 kV accelerating voltage with 1.5 nA beam current and 50 s dead time. Studies were carried out using backscattered images to ensure the maximum output information. The contrast in the image produced is determined by the atomic number of the elements in the sample. the shows the distribution of different chemical phases in the sample and produces valuable information on the morphology of the studied samples. The chemical composition of Sumuide natural and artiﬁcial beachrock samples


Introduction
Sandy beaches represent almost 1/3 of the ice-free global coastline [1], hosting variable socioeconomic activities related to residency, recreation, tourism, industry, agriculture, aquaculture and fishing, conservation, military and even energy production plants [2,3].
Beach erosion poses a threat for all relevant stakeholders, and particularly for the tourism industry, according to the World Tourism Organization [4]. According to a recent study by Vousdoukas et al. [5], 13.6-15.2% of the world's sandy beaches might face beach regression by 2050, while these numbers may rise up to 35.7-49.5% by the end of 2100. The effects of coastal erosion will be accelerated by the effects of climate change, such as the intensity of storms and the global sea level rise, and by non-climatic factors, such as the reduction in the sediment supply associated with the anthropogenic modification of rivers and coastlines [6], whilst management will be complicated by concerns over hard engineering sea protection measures [7][8][9][10].
Traditionally, hard engineering structures, such as seawalls, groins, piers, etc., have been used to manage storms and tides for the protection of developments within the coastal zone [11]. However, these solutions are expensive, require long time periods and the engineering of large amounts of materials, and last but not least, as demonstrated in many cases, instead of preventing they often accelerate erosion at other nearby locations [6,7,[11][12][13]. Furthermore, these techniques have a high impact on the natural coastal scenery, making it less appealing. Considering the above, alternative soft engineering techniques are needed to work in conjunction with natural coastal processes for a future with a sustainable coastal environment. The IPCC [14] has placed a greater emphasis on adaptation rather than impacts, reflecting a growing focus on integrated approaches by reducing risks that rely on flexible adaptation options and management [15]. A number of adaptation measures have been successfully implemented globally against coastal impacts and risks predominantly as a response to the current coastal risk or disasters experienced [16]. These need to be effective regardless of how environments change. Coastal management needs to shift to being more proactive than reactive, with a focus on planning then implementation, by emphasizing resilience, cost-effectiveness and working with nature. Furthermore, adaptive, sustainable planning should be undertaken in a wider socioeconomic development framework, considering societal needs, which often are more immediate than climate change.
Beachrocks are most commonly found in the Mediterranean and Caribbean Seas, the tropical and subtropical Atlantic coasts, Brazil, Japan, Australia, as well as the atolls of the Pacific and Indian Oceans [17,32]. A particular characteristic of sandy beaches that host beachrocks is that they are less impacted from erosional processes and maintain their morphological and sedimentological characteristics [19,20,33,34]. Based on their protective role, recent studies have introduced an innovative, ecofriendly and sustainable technique to create artificial beachrocks in the laboratory by using microbial-induced carbonate precipitation (MICP) [35][36][37][38]. The MICP technique is a promising method where bacteria precipitate calcium carbonate and modify soil characteristics, resulting in soil consolidation and stabilization. Using MICP, it is possible to imitate the formation of natural beachrocks as countermeasure against erosion affecting sandy beaches by creating artificial rocks as a new soft engineering protection measure. Biocementation technology is a promising technology due to its suitability for field application [37]. The idea of creating artificial beachrocks in vulnerable beaches as an ecofriendly coastal sustainable application is to zero the CO 2 emissions as this methodology does not involve concrete, energy-less consuming, and this method uses natural energy sources by using local sand (in situ), thus achieving the reduction of building materials.
There are many variations of applying the MICP technique [39,40], and particularly, the urea hydrolysis method is the most protrusive for beachrocks creation. The MICP urea hydrolysis technique is described by reactions 1, 2 and 3 [41]: In reaction 1, urea is hydrolyzed by urease (this reaction is called ureolysis) to produce carbamate and ammonium ions. In reaction 2, we observe the carbamate ion being hydrolyzed and producing an ammonia molecule and bicarbonate. Finally, in reaction 3, the diluted calcium ions are reacting with bicarbonate and ammonia to produce pre-calcium carbonate, which precipitates and forms the cement.
In this context, this study aims to provide an insight into a new soft engineering solution for protecting the vulnerable coastal zone from erosion. In order to achieve the aforementioned, we studied the beachrock cementation process by comparing the characteristics of natural beachrocks and the in vitro artificial beachrocks in terms of their mineralogical and geochemical properties. For the purposes of this study, two case studies are examined from different geological and geographical settings: Corinth, Greece and Okinawa Island, Japan. For the creation of artificial beachrocks, samples of local sand and bacteria of both study areas were used for three MICP experiments. A multi-analytical approach was used for the study of beachrocks, including petrographic microscopy, a scanning electron microscope (SEM) equipped with energy dispersive X-ray microanalysis (EDX) and X-ray diffraction (XRD).

Diolkos, Corinth, Greece
The Diolkos site is located at the Gulf of Corinth (Figure 1), a prominent tectonic and geomorphological structure, in the central part of Greece, segregating the Peloponnese to the south and Central Greece to the north. Most of Corinth's geological basement consists of Triassic to Jurassic grey limestones of the Sub-Pelagonian zone. The Corinth canal is composed of a series of faulted Pliocene-Pleistocene marls, limestones, sandstones and conglomerates [42].
The Diolkos site lies on the south coasts of the Isthmus canal. It is characterized by an extend beachrock outcrop, which is divided by the Corinth canal into the north and south. The beachrocks are located approximately 1 m above sea level (a.s.l.) [43]. The small beaches hosting the beachrocks are characterized by medium-sized grains to gravels. The studied beach has a North-South orientation with a 10 • inclination. At the southern beachrock outcrop, the remnants of Diolkos are visible. Diolkos was an ancient slipway used for transporting ships from the Corinth Gulf to Saronic Gulf and vice versa. This road was used for transporting ships with the help of a wheeled vehicle, where the ships were moored, once exempt from their cargo, in the docks of Cenchreae or Lechaion.

Figure 1.
Location of study areas. In the west is Diolkos, Corinth, Greece, while in the east is Sumuide, Okinawa Island, Japan.

Sumuide, Okinawa, Japan
Okinawa is the largest of the Okinawa Islands and the Ryukyu Islands of Japan. The island covers an area of 1206 m 2 and it is located 640 km south from the main island of Japan. The Ryukyu Islands extend from Kyushu in the north, to Taiwan in the south and exceed 1200 km in length.
Covering an area of about 150,000 km 2 , the Okinawa Trough is an active back-arc rifting basin between the East China Sea (ECS) Sheff Basin/Taiwan-Sinzi belt and the Ryukyu arc, since at least the late Miocene era [44], and it is the youngest back-arc rifting belt in the East China Sea. Although the Okinawa Trough mainly consists of igneous/volcanic rocks and Miocene sedimentary rocks, its basement comprises Paleogene and Mesozoic sedimentary sequences. The Ryukyu group is composed of a large number of reef complex deposits, with proximal water coral limestone and distal rhodolith and detrital limestones [45].
Beachrock outcrops are regularly distributed around Okinawa Island. Many studies have been published in recent years about beachrocks in Okinawa and their formation [20,31,46,47]. Evelpidou et al. [20] studied seven beachrock sites in the central western, south, central eastern and north parts of Okinawa Island. All beachrock occurrences extended no more than 1 m a.s.l. to a few centimeters below sea level (b.s.l.). In this study, we focus on the beachrock outcrop at Sumuide site, which is located in the central northern part of Okinawa Island (Figure 1).

Fieldwork
Field investigation included detailed spatial mapping of the beachrock outcrops in both studied sites. Transects were carried out in order to (i) measure the width and elevation/depth of beachrock slabs (with respect to mean sea-level) and (ii) sample the top beds of the front (seaward) and the end (landward) of each beachrock slab [19,20,[48][49][50]. At the Sumuide site (Okinawa, Japan), GPS measurements were performed with an accuracy of Figure 1. Location of study areas. In the west is Diolkos, Corinth, Greece, while in the east is Sumuide, Okinawa Island, Japan.

Sumuide, Okinawa, Japan
Okinawa is the largest of the Okinawa Islands and the Ryukyu Islands of Japan. The island covers an area of 1206 m 2 and it is located 640 km south from the main island of Japan. The Ryukyu Islands extend from Kyushu in the north, to Taiwan in the south and exceed 1200 km in length.
Covering an area of about 150,000 km 2 , the Okinawa Trough is an active back-arc rifting basin between the East China Sea (ECS) Sheff Basin/Taiwan-Sinzi belt and the Ryukyu arc, since at least the late Miocene era [44], and it is the youngest back-arc rifting belt in the East China Sea. Although the Okinawa Trough mainly consists of igneous/volcanic rocks and Miocene sedimentary rocks, its basement comprises Paleogene and Mesozoic sedimentary sequences. The Ryukyu group is composed of a large number of reef complex deposits, with proximal water coral limestone and distal rhodolith and detrital limestones [45].
Beachrock outcrops are regularly distributed around Okinawa Island. Many studies have been published in recent years about beachrocks in Okinawa and their formation [20,31,46,47]. Evelpidou et al. [20] studied seven beachrock sites in the central western, south, central eastern and north parts of Okinawa Island. All beachrock occurrences extended no more than 1 m a.s.l. to a few centimeters below sea level (b.s.l.). In this study, we focus on the beachrock outcrop at Sumuide site, which is located in the central northern part of Okinawa Island (Figure 1).

Fieldwork
Field investigation included detailed spatial mapping of the beachrock outcrops in both studied sites. Transects were carried out in order to (i) measure the width and elevation/depth of beachrock slabs (with respect to mean sea-level) and (ii) sample the top beds of the front (seaward) and the end (landward) of each beachrock slab [19,20,[48][49][50]. At the Sumuide site (Okinawa, Japan), GPS measurements were performed with an accuracy of ±5 cm and the elevation/depth of beachrocks were measured using a 3 m metallic bar with 20 cm division. At the Diolkos site (Corinth, Greece), both coordinates and elevation/depth were measured using a DGPS-GNSS Spectra SP60 system, with an accuracy of ±3 cm.
Beachrock samples were collected from both sites; 6 samples were obtained from Diolkos, due to the irregular shape of the outcrop, and 2 samples were from Sumuide. In addition, samples of sand were collected from the overlying sediment of beachrocks from both studied sites to be used for solidification testing to create the in vitro beachrocks. The samples were stored in sterile tubes to avoid any contamination.

Screening and Cultivation of Bacteria
In our study, we used the bacteria Micrococcus yunnanensis sp. (Bacteria B1) isolated from the beach sand of the Diolkos site [51] and the bacteria Pararhodobacter sp. (Bacteria B2) from the Sumuide site [52]. Both bacteria species were identified by 16S rRNA gene analysis and were used for the calcium carbonate precipitation. A ZoBell2216E medium (5.0 gr/L polypeptone, 1.0 gr/L yeast extract, and 0.1 gr/L FePO 4 was prepared in artificial seawater (Table 1), adjusted to pH 7.6-7.8) was used as the culture medium for cultivating the bacteria. The isolated bacteria were pre-cultured in 5 mL medium at 30 • C with shaking at 160 rpm for 24 h. One milliliter of the pre-culture was inoculated into 100 mL of ZoBell2216E medium incubated at 30 • C while shaking it at 160 rpm. During cultivation, bacteria cell concentration was determined by measuring the absorbance (Optical Density, OD) of the suspension using a UV-vis spectrophotometer (V-730, JASCO Corporation, Tokyo, Japan) at a 600 nm wavelength (OD 600 ). During cultivation, we used a 0.5 M ZoBell2216E medium for the bacteria Micrococcus yunnanensis sp. and Pararhodobacter sp. and a 0.3 M ZoBell2216E medium for only Pararhodobacter sp. Additionally, we applied a urease activity test using Cresol Red solution. By measuring the bacteria concentration OD 600 , we obtained for the bacteria Micrococcus yunnanensis sp. the growth curve of the microbial strain. Two growth curves of bacteria Pararhodobacter sp. were obtained, one for the 0.5 M medium solution, which led to a high-concentration bacteria solution, and one for the 0.3 M medium solution, which led to a low-concentration solution ( Figure 2).

Experimental Conditions
The MICP artificial beachrock experiment was implemented by using three different solidification scenarios. From hereinafter, the three scenarios will be numbered as experiments 1, 2 and 3. Experiment 1 involved beach sand samples from Diolkos area and the bacteria Micrococcus yunnanensis sp. (B1). For the experiment setting, 1 kg of beach sand was sieved using six mesh sizes to separate gravel (>2 mm), sand (1 mm, 500 μm, 250 μm, 125 μm, 63 μm) and silt/clay particles (<63 μm). Samples were oven-dried at 80 °C. For the artificial beachrock experiment, the grain size composition used corresponds to the granulometry of Diolkos beach, i.e., 26.5% gravels, 72% sand and 0.5% silt/clays. Furthermore, the bacteria density and the CaCO3 content were maintained as stable during the experiment, operated at 25 °C. Experiments 2 and 3 concerned sand samples from the Sumuide site and the bacteria Pararhodobacter sp. (B2). The sand samples consisted of calcareous material, which was sieved using two mesh sizes to separate gravel (>2 mm) and coarse and medium sand (1 mm). The sand used for both experiments had a grain size of 1 mm. Additionally, for both experiments (2, 3) the CaCO3 content and temperature (30 °C) were kept stable. However, the two experiments differed in the concentration of bacteria solution; for experiment 2 a high-concentration solution was used, while for experiment 3, we used a low one.

Syringe Solidification Test
Sterilized sand samples from Diolkos and Sumuide beaches were prepared in syringes of 50 mL and 35 mL volume, respectively. In the syringes, 85.5 gr of Diolkos sand (experiment 1) and 40 gr of coral sands (experiment 2 and 3) were used, along with three bacteria reagents.
During the solidification experiments, in order to prepare the bacteria B1 reagent for experiment 1 and the two bacteria reagents of high and low bacteria B2 concentration for experiment 2 and 3, respectively, the density of bacteria and their growth was determined in the solutions using the Optical Density value of 600 nm by UV-VIS spectrophotometer (UV-2500PC SHIMADZU). The number of bacteria in the solution of experiment 1 were measured for 9 days and in the experiments 2 and 3 for 24 h.
Following the experiment, ZoBell2216E culture medium solution was prepared using artificial seawater as solvent. The culture medium and artificial seawater solution was inoculated with 0.1 gr per 100 mL solution of bacteria B1 for experiment 1, and with bacteria B2 for experiments 2 and 3. Then, all were incubated at 30 °C while shaking them at 80 rpm for 3 days.

Experimental Conditions
The MICP artificial beachrock experiment was implemented by using three different solidification scenarios. From hereinafter, the three scenarios will be numbered as experiments 1, 2 and 3. Experiment 1 involved beach sand samples from Diolkos area and the bacteria Micrococcus yunnanensis sp. (B1). For the experiment setting, 1 kg of beach sand was sieved using six mesh sizes to separate gravel (>2 mm), sand (1 mm, 500 µm, 250 µm, 125 µm, 63 µm) and silt/clay particles (<63 µm). Samples were oven-dried at 80 • C. For the artificial beachrock experiment, the grain size composition used corresponds to the granulometry of Diolkos beach, i.e., 26.5% gravels, 72% sand and 0.5% silt/clays. Furthermore, the bacteria density and the CaCO 3 content were maintained as stable during the experiment, operated at 25 • C. Experiments 2 and 3 concerned sand samples from the Sumuide site and the bacteria Pararhodobacter sp. (B2). The sand samples consisted of calcareous material, which was sieved using two mesh sizes to separate gravel (>2 mm) and coarse and medium sand (1 mm). The sand used for both experiments had a grain size of 1 mm. Additionally, for both experiments (2, 3) the CaCO 3 content and temperature (30 • C) were kept stable. However, the two experiments differed in the concentration of bacteria solution; for experiment 2 a high-concentration solution was used, while for experiment 3, we used a low one.

Syringe Solidification Test
Sterilized sand samples from Diolkos and Sumuide beaches were prepared in syringes of 50 mL and 35 mL volume, respectively. In the syringes, 85.5 gr of Diolkos sand (experiment 1) and 40 gr of coral sands (experiment 2 and 3) were used, along with three bacteria reagents.
During the solidification experiments, in order to prepare the bacteria B1 reagent for experiment 1 and the two bacteria reagents of high and low bacteria B2 concentration for experiment 2 and 3, respectively, the density of bacteria and their growth was determined in the solutions using the Optical Density value of 600 nm by UV-VIS spectrophotometer (UV-2500PC SHIMADZU). The number of bacteria in the solution of experiment 1 were measured for 9 days and in the experiments 2 and 3 for 24 h.
Following the experiment, ZoBell2216E culture medium solution was prepared using artificial seawater as solvent. The culture medium and artificial seawater solution was inoculated with 0.1 gr per 100 mL solution of bacteria B1 for experiment 1, and with bacteria B2 for experiments 2 and 3. Then, all were incubated at 30 • C while shaking them at 80 rpm for 3 days.
Meanwhile, 80 gr of Diolkos sand for experiment 1 and 40 gr of coral sand for experiments 2 and 3 were dried at 100 • C for 2 days and placed in a 50 mL and 35 mL syringe, respectively. Subsequently, for experiment 1, we prepared 25 mL of B1 bacterial culture along with 30 mL of 0.5 M solidification solution (Table 2). For experiments 2 and 3, 16 mL of B2 bacterial cultures (high and low concentration) along with 20 mL of solidification solution were sequentially injected into the syringe and drained out, while having the sand saturated with 2 mL of solution above the surface of the cement. Table 2. Chemical composition of a 0.5 M solidification solution used during the artificial beachrock experiment.

Chemical Components Amounts
Artificial seawater 100 mL Nutrient Broth 0.3 gr NH 4 Cl 1 gr (NH 2 ) 2 CO 3 gr NaHCO 3 0.212 gr CaCl 2 5.55 gr After curing the sand samples of experiments 1, 2 and 3 with their corresponding bacterial solutions, the consolidation solution was injected from the top of the syringe and drained out from the bottom at fixed intervals of 24 h for 14 days. The Ca 2+ concentration and pH of the drainage outlet were also measured to determine temporal variations of these parameters in the samples. pH and Ca 2+ are indicators of the urease hydrolysis taking place in the samples. For Ca 2+ measurement, a LAQUA twin (HORIBA) was used, while for the pH, we used an electronic portable pH meter. For experiment 1, the Ca 2+ and pH measurements were taken daily. For experiments 2 and 3, measurements were taken on days 3, 6, 9, 12, and 14 of the experiment.
It should be noted that in the middle of the experiment (day 7), we reinjected the bacteria solution in order to rejuvenate the bacteria in the syringe test.

Solidification Determination
After 14 days of the solidification experiments, the samples were extracted from the syringes. A needle penetration device (SH-70, Maruto Testing Machine Company, Tokyo, Japan) was used to calculate the penetration inclination (Np) and estimate the unconfined compressive strength (Newton/mm = Pressure N/m 2 , needle's head area is inconsiderable) [36][37][38]52]. The UCS was estimated by needle penetration test.
All consolidated samples were approximately 7 cm in length. The needle penetration test was applied on 3 parts of the sample: upper, middle, and bottom, with a mean step of 2 cm.

Mineralogy and Geochemical Analysis
Mineralogical and geochemical analyses were conducted at the laboratory of the Department of Mineralogy and Petrography, Institute of Geology and Mineral Exploration, Athens, Greece and the laboratory of Economic Geology and Geochemistry at the Faculty of Geology and Geoenvironment of the National and Kapodistrian University of Athens.
The natural beachrock samples from Diolkos and Sumuide and their corresponding artificial samples were analyzed for the determination of their mineralogical composition and textural characteristics with a LEICA-DMLP (Leica Microsystems GmbH, Wetzlar, Germany) petrographic microscope equipped with a digital camera. The identification of minerals by their color is based on the method of Michel-Lévy Interference Color Chart.
The bulk mineralogy of the natural beachrock samples was analyzed using the X-ray diffraction (XRD) method. In the case of natural Diolkos samples, a Panalytical X'pert-Pro X-ray diffractometer was used, equipped with a Cu X-ray tube (Kα of Cu, λ = 1.5405 Å), a graphite monochromator, an applied voltage of 30 kV and a 40 mA current. The random powder mounts of samples, prepared by back loading, were scanned from 2 • to 70 • 2θ. Powder diffraction data were collected at room temperature. Data were evaluated with the X'Pert High-Score (Version 2004) (PANalytical B.V., Almelo, The Netherlands). Further XRD analyses were also conducted on the natural and artificial beachrock samples from Sumuide. X-ray diffraction measurements were carried out on a Bruker Model 5005 X-ray diffractometer in combination with the DIFFRAC plus software package. The diffractometer was operated using Cu Kα radiation at 40 kV and 40 mA with a graphite monochromator. In Sumuide cases, the XRD output files were evaluated for mineralogical identifications using the EVA ® software provided by Bruker (DIFFRACplus software EVA, Bruker AXS GmbH) (Bruker Corporation (2006), DIFFRACplus EVA v12.0.) and managed with the PDF-2 database (International Centre for Diffraction Data, Newtown Square, PA, USA).
The chemical composition of Diolkos natural beachrock minerals was examined under scanning electron microscopy (SEM) using a JEOL JSM-5600 equipped with an Oxford Link Energy Dispersive Spectrometer (EDS) (Oxford Instruments). Microanalyses were performed on epoxy resin-impregnated polished and carbon-coated thin sections. The measured concentrations were automatically corrected for atomic number, absorption in the sample, fluorescence and dead time (the ZAF correction) using the INCA software. The chemical composition of the minerals was determined using natural minerals and synthetic oxide standards, and 20 kV accelerating voltage with 1.5 nA beam current and 50 s dead time. Studies were carried out using backscattered images to ensure the maximum output of information. The contrast in the image produced is determined by the atomic number of the elements in the sample. Therefore, the image shows the distribution of different chemical phases in the sample and produces valuable information on the morphology of the studied samples. The chemical composition of Sumuide natural and artificial beachrock samples were studied at Hokkaido University laboratories under SEM-EDX using a SHIMADZU SUPERS CAN SSX-550 and using the same aforementioned analytical methodology under 15 kV accelerating voltage.
The concentrations of major and trace elements in bulk samples of Diolkos natural beachrocks were defined using energy dispersive X-ray florescence spectroscopy (EDS-XRF, S4 Ranger, Bruker). Loss on ignition (LOI) was determined from the weight loss after heating 1 gr of sample (previously dried at 105 • C) for 1 h at 1000 • C. XRF analysis was not applied on Sumuide natural beachrock samples due to the main calcite composition and the difficulty to distinguish the cement composition from the calcareous beach grains.

Beachrock Distribution
At the Diolkos site, the beachrock has a mean dip of 12.5 • seawards. Its maximum width is about 30 m. The outcrop's maximum height above the mean sea level is 1.10 m. The outcrop has no evidence of erosional rims (channels) perpendicular to the shoreline on its surface. The channel of Corinth is protected by a sea wall where the examined beachrock is located. In addition, manmade structures have been noticed to be incorporated into the beachrock outcrops.
Sumuide beach is oriented West-East and the examined beachrock extends parallel to the coast with an average length of 127 m and a seaward dip of 3.5 • . Its mean width is 12 m with a maximum height above the mean sea level of about 70 cm. Quarrying marks are evident on the outcrop, while there are also erosional features from the wave energy.

Beachrock Lithology
Diolkos beachrock samples are characterized by a coherent pattern with sub-rounded and well-to-medium sorted grains and the general absence of bioclasts (<3%). The lithoclasts mainly consist of quartz, calcite, dolomite, plagioclase, K-feldspar.
For Sumuide beachrock, the seaward sample, Sum1, has poorly rounded and medium sorted lithoclasts, while its lithology consists mostly of calcite, quartz, dolomite and mica with a high presence of bioclasts (≥60%). On the other hand, the landward sample, Sum2, has medium rounded and sorted lithoclasts and grains, which consist mostly of calcite and aragonite with a high presence of bioclasts (≥50%) ( Table 3). Intertidal zone, physicochemical precipitation, medium microbial activity.

Cement Composition
The examination of the beachrock cements from Diolkos showed that the micritic HMC [(Ca,Mg)CO 3 ] cement was the most dominant in all samples, identified using backscattered electron images (BSE) and EDS analyses. In the samples Kodi1, Kodi2, Kodi3 and Kodi4, the cement forms a thin isopachous coating around the sediment grains. On the other hand, the samples Kodi5 and Kodi6 are characterized by a thick isopachous coating of micritic HMC cement. Following the coating cementation, a brown micritic cement forming an outer cover was identified in almost all samples, with the exception of Kodi3. A pore filling matrix cement was observed, consisting of very fine sedimentary particles (5-20 µm) and micritic HMC in the samples Kodi1, Kodi2 and Kodi3. In the samples Kodi3 and Kodi5, a pellet cement formation was identified, while in the latter meniscus, cement formation was also noted (Table 3) (Figure 3).
Both samples from Sumuide (Sum1 and Sum2) were characterized by the same cement type of HMC, and by isopachous micritic and sparitic crystals (rhomboidal crystals) along with needle-like crystals of aragonite. Furthermore, matrix cement was also identified as pore filling. The cement texture can be generally characterized as micritic with the presence of interclasts, while also forming meniscus and peloidal concentrations (Figure 4).

Mineralogical and Chemical Analysis
Detailed X-ray diffraction analysis (XRD) of Diolkos samples showed that they are characterized by the presence of terrigenous minerals such as quartz, calcite, Mg-calcite, dolomite, plagioclase, K-feldspar and traces of serpentine and clay minerals (illite, chlorite). Samples Kodi2 and Kodi3 are more abundant in Mg-calcite than the rest. The content    (d) micritic cement that is coating a microfossil and is precipitated inside its vents.

Mineralogical and Chemical Analysis
Detailed X-ray diffraction analysis (XRD) of Diolkos samples (Figures S1-S7) showed that they are characterized by the presence of terrigenous minerals such as quartz, calcite, Mg-calcite, dolomite, plagioclase, K-feldspar and traces of serpentine and clay minerals (illite, chlorite). Samples Kodi2 and Kodi3 are more abundant in Mg-calcite than the rest. The content in chlorite and serpentine is probably due to the presence of ultramafic sediments that are derived from ophiolites. Additionally, all Diolkos samples are abundant in calcite.
The results from the X-ray diffraction analysis of sample Sum2 from Sumuide showed the presence of carbonate minerals, such as Mg-calcite and aragonite.
The geochemistry of the beachrocks is in full accordance with the mineralogical/lithological characteristics of the samples. Table 3  in chlorite and serpentine is probably due to the presence of ultramafic sediments that are derived from ophiolites. Additionally, all Diolkos samples are abundant in calcite.
The results from the X-ray diffraction analysis of sample Sum2 from Sumuide showed the presence of carbonate minerals, such as Mg-calcite and aragonite.
The geochemistry of the beachrocks is in full accordance with the mineralogical/lithological characteristics of the samples. Table 3 presents the chemical composition of selected Diolkos samples, with the concentrations in major and trace elements of the samples analyzed using XRF. The mean concentration value of CaO of about 38.5% wt and the mean concentration of MgO of about 2.9% wt derive from both the sediment grains and the cement ( Figure 5). The mean concentration of SiO2 of about 22% wt derives only from the silicate sediment composition. The mean values of LOI (loss on ignition) reach a value of 33% wt. LOI's origination derives from the ignited carbonates. Thus, we count it as a part of both sediment and cement chemical imprint.
Trace element concentrations (ppm) provided notable results concerning the presence of Cr and Ni, which show a higher concentration in the Kodi2 sample compared to Kodi6. The samples Kodi2 and Kodi6 showed Sr values of 591 ppm and 640 ppm, respectively. Strontium concentrations in water determine the sea water impact on the cementation process of beachrocks [53,54] and also reflect the weathering history of sediments [55].

Microbial Population Count
In the case of Diolkos (experiment 1), we created a treatment solution of B1 bacteria, and their cell density was determined every day for a duration of 9 days. On day T0, the solution showed a 1400 nm (OD600) of B1, while it topped at 3790 nm (OD600) on day T3 and at 2200 nm on day T9 (OD600) (Figure 2). Trace element concentrations (ppm) provided notable results concerning the presence of Cr and Ni, which show a higher concentration in the Kodi2 sample compared to Kodi6. The samples Kodi2 and Kodi6 showed Sr values of 591 ppm and 640 ppm, respectively. Strontium concentrations in water determine the sea water impact on the cementation process of beachrocks [53,54] and also reflect the weathering history of sediments [55].

Microbial Population Count
In the case of Diolkos (experiment 1), we created a treatment solution of B1 bacteria, and their cell density was determined every day for a duration of 9 days. On day T0, the solution showed a 1400 nm (OD 600 ) of B1, while it topped at 3790 nm (OD 600 ) on day T3 and at 2200 nm on day T9 (OD 600 ) (Figure 2).
In the case of Sumuide (experiments 2, 3), the density of bacteria B2 in the treatment solution was analyzed at time T: 0 h, 3 h, 6 h, 12 h, and 24 h (Figure 2). The high bacteria concentration treatment solution exceeds the limit of 2.200 nm (OD 600 ) in the short time of 9 h. On the contrary, the low bacteria concentration treatment solution topped its measurement at 1.810 nm (OD 600 ) in 24 h. We also used the bacteria of the high (experiment 2) and low concentration (experiment 3) solutions in order to create colonies while counting them. In experiment 2, bacteria cultivation had grown 62 colonies, while in experiment 3 bacteria cultivation had grown 19 colonies.
The solidification solution that we used for all experiments was maintained at pH 7.6-7.8. We applied/injected treatment solution daily for 14 days. In the case of the ArDi1 sample, the pH value showed a general rise trend until day 5 (with an exception on day 2, where there was a sharp drop). From day 5 until 7, the pH value had dropped to 7.5. On the same day (7), we reinjected bacterial culture solution, and on day 8 we noted a rise of the pH up to 7.9. However, the pH continued to drop until day 10, followed by a gentle rise until day 14, reaching up to 7.7 (Figure 6a). concentration treatment solution exceeds the limit of 2.200 nm (OD600) in the short time of 9 h. On the contrary, the low bacteria concentration treatment solution topped its measurement at 1.810 nm (OD600) in 24 h. We also used the bacteria of the high (experiment 2) and low concentration (experiment 3) solutions in order to create colonies while counting them. In experiment 2, bacteria cultivation had grown 62 colonies, while in experiment 3 bacteria cultivation had grown 19 colonies.
The solidification solution that we used for all experiments was maintained at pH 7.6-7.8. We applied/injected treatment solution daily for 14 days. In the case of the ArDi1 sample, the pH value showed a general rise trend until day 5 (with an exception on day 2, where there was a sharp drop). From day 5 until 7, the pH value had dropped to 7.5. On the same day (7), we reinjected bacterial culture solution, and on day 8 we noted a rise of the pH up to 7.9. However, the pH continued to drop until day 10, followed by a gentle rise until day 14, reaching up to 7.7 (Figure 6a).
The pH value for both the ArSum1 and ArSum2 samples showed an increasing trend until day 3 of the experiment, and then decreased until day 6. On day 7, we reinjected bacterial culture solution in our experiment with the result of maintaining the pH until day 10. The dramatic change occurred on day 12, where a decreasing trend in the pH was observed (Figure 6b). In all experiments, we added 20,000 ppm of Ca 2+ daily. At time T = 0 days, the experiment had the initial content of 20,000 ppm of Ca 2+ . For sample ArDi1, the Ca 2+ concentration in its drainage is characterized by a low concentration until day 2, followed by a rise until day 5. Day 7 is characterized by a dramatic fall of the Ca 2+ concentration in the drainage, followed by a gentle rise until day 13 and concluding in day 14 with a decrease (Figure 7a).
The samples ArSum1 and ArSum2 are characterized by a constantly rising concentration of Ca 2+ in their drainage (Figure 7b). On the third day of experiments, we measured almost the same values of Ca 2+ in the outlet solution. A steady increase in concentration was observed until day 6. Even after the re-injection of bacterial culture solution on day 7, the ArSum2 sample tended to have an increase in the Ca 2+ concentration in its drainage until the final day of the experiment. The sample ArSum1 showed a more stable trend in Ca 2+ concentration in its drainage of 240 mg/L until the 12th day. On the final 2 days of the experiment, a rise in Ca 2+ was observed in drainage. The pH value for both the ArSum1 and ArSum2 samples showed an increasing trend until day 3 of the experiment, and then decreased until day 6. On day 7, we reinjected bacterial culture solution in our experiment with the result of maintaining the pH until day 10. The dramatic change occurred on day 12, where a decreasing trend in the pH was observed (Figure 6b).
In all experiments, we added 20,000 ppm of Ca 2+ daily. At time T = 0 days, the experiment had the initial content of 20,000 ppm of Ca 2+ . For sample ArDi1, the Ca 2+ concentration in its drainage is characterized by a low concentration until day 2, followed by a rise until day 5. Day 7 is characterized by a dramatic fall of the Ca 2+ concentration in the drainage, followed by a gentle rise until day 13 and concluding in day 14 with a decrease (Figure 7a).
The samples ArSum1 and ArSum2 are characterized by a constantly rising concentration of Ca 2+ in their drainage (Figure 7b). On the third day of experiments, we measured almost the same values of Ca 2+ in the outlet solution. A steady increase in concentration was observed until day 6. Even after the re-injection of bacterial culture solution on day 7, the ArSum2 sample tended to have an increase in the Ca 2+ concentration in its drainage until the final day of the experiment. The sample ArSum1 showed a more stable trend in Ca 2+ concentration in its drainage of 240 mg/L until the 12th day. On the final 2 days of the experiment, a rise in Ca 2+ was observed in drainage.

Mineralogical Study and Geochemical Analysis of Artificial Beachrocks
The artificial beachrocks were formed in vitro; thus, their physical characteristics were expected to be similar to the beach material. In the case of Diolkos sample (ArDi1), the lithoclasts are sub-rounded and poorly sorted, with a small contribution of bioclasts (<10%). The grains consisted of quartz, calcite, dolomite, albite and serpentine. The two artificial samples of Sumuide also showed a similar pattern to the corresponding natural beachrock: rounded and well-sorted grains with a high contribution of bioclasts (>60%), while the grains consisted of calcite, Mg-calcite and aragonite ( Table 3).
The microscopic analysis of the three artificial beachrock samples showed that the sediment grains are similar in shape and composition with the natural beachrocks of the corresponding beaches. The cement of sample ArDi1, which was produced by the application of bacteria Micrococcus yunnainensis sp. and sand from the Diolkos area, is characterized by micritic and spiritic crystals of calcite in various sizes, from 10 μm to 100 μm. The crystal size and arrangement are regulated by the temperature, the calcium saturation and the gravitational forces. Additionally, a discontinuous fan shape grain coating was observed, along with microcrystalline calcite serving as pore filling with thick crystals of

Mineralogical Study and Geochemical Analysis of Artificial Beachrocks
The artificial beachrocks were formed in vitro; thus, their physical characteristics were expected to be similar to the beach material. In the case of Diolkos sample (ArDi1), the lithoclasts are sub-rounded and poorly sorted, with a small contribution of bioclasts (<10%). The grains consisted of quartz, calcite, dolomite, albite and serpentine. The two artificial samples of Sumuide also showed a similar pattern to the corresponding natural beachrock: rounded and well-sorted grains with a high contribution of bioclasts (>60%), while the grains consisted of calcite, Mg-calcite and aragonite ( Table 3).
The microscopic analysis of the three artificial beachrock samples showed that the sediment grains are similar in shape and composition with the natural beachrocks of the corresponding beaches. The cement of sample ArDi1, which was produced by the application of bacteria Micrococcus yunnainensis sp. and sand from the Diolkos area, is characterized by micritic and spiritic crystals of calcite in various sizes, from 10 μm to 100 μm. The crystal size and arrangement are regulated by the temperature, the calcium saturation and the gravitational forces. Additionally, a discontinuous fan shape grain coating was observed, along with microcrystalline calcite serving as pore filling with thick crystals of

Mineralogical Study and Geochemical Analysis of Artificial Beachrocks
The artificial beachrocks were formed in vitro; thus, their physical characteristics were expected to be similar to the beach material. In the case of Diolkos sample (ArDi1), the lithoclasts are sub-rounded and poorly sorted, with a small contribution of bioclasts (<10%). The grains consisted of quartz, calcite, dolomite, albite and serpentine. The two artificial samples of Sumuide also showed a similar pattern to the corresponding natural beachrock: rounded and well-sorted grains with a high contribution of bioclasts (>60%), while the grains consisted of calcite, Mg-calcite and aragonite ( Table 3).
The microscopic analysis of the three artificial beachrock samples showed that the sediment grains are similar in shape and composition with the natural beachrocks of the corresponding beaches. The cement of sample ArDi1, which was produced by the application of bacteria Micrococcus yunnainensis sp. and sand from the Diolkos area, is characterized by micritic and spiritic crystals of calcite in various sizes, from 10 µm to 100 µm. The crystal size and arrangement are regulated by the temperature, the calcium saturation and the gravitational forces. Additionally, a discontinuous fan shape grain coating was observed, along with microcrystalline calcite serving as pore filling with thick crystals of aragonite ( Figure 9). The samples ArSum1 and ArSum2 were created by using coral sand and Pararhodobacter sp. bacteria of different concentrations from Sumuide. The two samples consisted of similar grain shape and composition to the local beachrock. Their cement was made of particularly well-developed aragonite crystals with lengths of around 10 µm to 20 µm (Figure 10).

Discussion
Beachrocks undoubtedly perform the role of a natural breakwater protecting the coast from erosion [31,56,57]. In this study, we identified the beachrock formation mechanism of Diolkos area, Corinth, Greece and Sumuide, Okinawa island, Japan. Additionally, analytical data were collected with the purpose of investigating the innovative method of MICP for creating artificial beachrocks as a proposal of a soft engineering countermeasure of sandy beach erosion.
Diolkos beachrock outcrop has a very compacted and thick morphology, which was also confirmed through optical and electron microscopy. The Diolkos beachrock outcrop has a mean thickness of 3 m, in contrast with the outcrop of Sumuide which was about 50 cm. In contrast with Diolkos' 10° inclination, Sumuide beachrock shows a very gentle inclination, almost parallel with the sea level. The sediment texture of the studied beachrocks is connected with the recent sedimentary processes of each area that supply material from the corresponding weathered bedrock. In Diolkos' case, there was a variety of consolidated lithoclasts, minerals and mineral grains with differences in their material density and hardness. The heterotropic erosion and sleeking of minerals has led to a variety of sizes in the consolidated sediment, from silt and very fine sand to pebbles and gravels. On the other hand, Sumuide beachrock sediment derives from the local coral reef of Okinawa Island and thus its texture is uniformly sandy with fossilized skeleton parts of foraminifera and coral parts.

Beachrock Cement
The cement of Diolkos beachrock is a typical high magnesium calcite (HMC) in micritic and sparitic crystal forms. Matrix cement was noted consisting of very fine sediment particles (silt) and micritic cement. The HMC micritic cement indicates marine provenance, but the matrix formation in the pores of the sediment indicates the mixing of sea and fresh water [18]. Combining the information of the cement type and cement texture, it is clear that Diolkos beachrock outcrop was formed by the combination of marine water and fresh water with a higher influence by the marine phase taking place in the upper intertidal zone.
The XRD analysis showed that the Diolkos beachrock is more abundant in Mg-calcite at its inner part (landward; samples Kodi2 and Kodi3) compared to its seaward part (samples Kodi4, Kodi5 and Kodi6). This difference in Mg-calcite was also noted by the XRF analysis and the correlation diagram of MgO/CaO (Figure 5c). Additionally, the XRF analysis showed that the inner part of Diolkos beachrock had high concentrations in Cr and Ni ( Figure 5) that may have derived from the ophiolite outcrops, north of Loutraki city through torrents and small river estuaries.

Discussion
Beachrocks undoubtedly perform the role of a natural breakwater protecting the coast from erosion [31,56,57]. In this study, we identified the beachrock formation mechanism of Diolkos area, Corinth, Greece and Sumuide, Okinawa island, Japan. Additionally, analytical data were collected with the purpose of investigating the innovative method of MICP for creating artificial beachrocks as a proposal of a soft engineering countermeasure of sandy beach erosion.
Diolkos beachrock outcrop has a very compacted and thick morphology, which was also confirmed through optical and electron microscopy. The Diolkos beachrock outcrop has a mean thickness of 3 m, in contrast with the outcrop of Sumuide which was about 50 cm. In contrast with Diolkos' 10 • inclination, Sumuide beachrock shows a very gentle inclination, almost parallel with the sea level. The sediment texture of the studied beachrocks is connected with the recent sedimentary processes of each area that supply material from the corresponding weathered bedrock. In Diolkos' case, there was a variety of consolidated lithoclasts, minerals and mineral grains with differences in their material density and hardness. The heterotropic erosion and sleeking of minerals has led to a variety of sizes in the consolidated sediment, from silt and very fine sand to pebbles and gravels. On the other hand, Sumuide beachrock sediment derives from the local coral reef of Okinawa Island and thus its texture is uniformly sandy with fossilized skeleton parts of foraminifera and coral parts.

Beachrock Cement
The cement of Diolkos beachrock is a typical high magnesium calcite (HMC) in micritic and sparitic crystal forms. Matrix cement was noted consisting of very fine sediment particles (silt) and micritic cement. The HMC micritic cement indicates marine provenance, but the matrix formation in the pores of the sediment indicates the mixing of sea and fresh water [18]. Combining the information of the cement type and cement texture, it is clear that Diolkos beachrock outcrop was formed by the combination of marine water and fresh water with a higher influence by the marine phase taking place in the upper intertidal zone.
The XRD analysis showed that the Diolkos beachrock is more abundant in Mg-calcite at its inner part (landward; samples Kodi2 and Kodi3) compared to its seaward part (samples Kodi4, Kodi5 and Kodi6). This difference in Mg-calcite was also noted by the XRF analysis and the correlation diagram of MgO/CaO (Figure 5c). Additionally, the XRF analysis showed that the inner part of Diolkos beachrock had high concentrations in Cr and Ni ( Figure 5) that may have derived from the ophiolite outcrops, north of Loutraki city through torrents and small river estuaries.
Conversely, Sumuide beachrock is a different case. It is characterized by a welldeveloped coating of micritic HMC, with an abundance of sparitic calcite crystals and aragonite up to 20 µm in size. The calcareous sediment composition favors the formation of calcite and coarse needle aragonite, which occasionally was recrystallized and formed smaller sparitic crystals. The coexistence of these different types of cement indicates a low intertidal/upper subtidal zone precipitation as it is mostly affected by the marine environment. These observations are in accordance with the XRD mineralogical analysis as the samples mainly consisted of Mg-calcite and aragonite. Additionally, it should be taken in account that tropical areas, such as Okinawa, have rainfalls with many millimeters of precipitation which can lead to a high water table level [58]. This leads to a mixed water (sea and fresh/meteoric water) cementation mechanism even if it occurs in the subtidal zone.

Artificial Beachrocks
The experiments for the artificial beachrocks were conducted taking into account two different considerations. During experiment 1 (sample ArDi1), the granulometry of the material used was similar to that of Diolkos beach. Conversely, during experiments 2 and 3, the granulometry of the samples was homogenous at 1 mm. The use of bacteria Micrococcus yunnanensis sp. (B1) and Paradohobacter sp. (B2) was defined due to their metabolic reaction that triggers the urease activity, which is followed by the CaCO 3 precipitation; this is the main component of the MICP method.
During the solidification treatment of the artificial beachrock samples, pH and Ca 2+ were measured from the outlet drainage. The reduction in pH during the treatment indicates the urease activity in the syringe samples (reaction 2). On the other hand, Ca 2+ showed a reduced rate in the outlet drainage, which is due to the precipitation of CaCO 3 (reaction 3) around the grains in the syringe. As the reaction proceeds, the calcium carbonate precipitates and forms aragonite and calcite cement.
The drainage of the ArDi1 sample showed high bacterial activity until the fifth day, the eighth day and during the last three days of experiment 1. The drainage of the ArSum1 sample showed higher values of pH and lower concentrations of Ca 2+ than ArSum2. Both samples had the same pH trend, which showed a high bacterial activity until day 6. On day 7, a rise in the bacterial urease activity was noted until day 9. Although the second injection was applied, the pH did not rise to the initial value. These results suggest a difference in the samples' consolidation and physicochemical parameters.
Using the UCS needle penetration test on the middle and bottom part of ArDi1 samples, it was evident that the cementation was strong. The ArDi1 sample had a variety of sediment sizes, and this factor was important for the circulation of the solution in the pores. The upper part of ArDi1 had consolidated around a pebble that was 2 mm wide, along with sand particles of different sizes; thus, the UCS needle penetration test could not be applied on this part.
The UCS needle penetration test also confirmed that the Okinawa sample ArSum1 showed higher strength values than ArSum2; however, both samples were more compacted on their upper part than their lower (Figure 8). This could be due to the fact that the bottom resident bacteria were partially able to participate in the entire urease activity process. At the same time, the higher concentration of organic material at the bottom of the syringe due to bacteria mortality may also locally change the bacteria's living conditions. SEM images and EDS analysis have shown that the ArSum1 sample is characterized by coarse, well-developed crystals of aragonite cement in comparison to ArSum2, which did not have well-developed aragonite crystals, but had also additionally formed calcite cement.
Even though Sumuide artificial beachrocks consisted of 1 mm sand particles, which facilitated the solution circulation in the pores, the strength of the material was inferior to Diolkos artificial beachrock. Additionally, the particles' inhomogeneity in the ArDi1 sample and the resulting different porosity led to the precipitation of matrix cement, which also enhances the reduction in the pores. These findings reveal that the understanding of bacteria ureolysis ability plays a more important role than the particles' homogeneity.
All experimental conditions applied in this study showed the ability to create artificial beachrocks with specific physical and geochemical characteristics. The results of the artificial beachrock experiments highlight their potential to be used as an adaptation measure against coastal erosion. It has been noted by various authors [19,20,22,33,34] that sandy coasts hosting beachrocks are less affected by coastal erosion as beachrocks act as natural breakwaters that mitigate wave energy. Given the need for ecofriendly, sustainable and cost efficient coastal management measures, the MICP method to create artificial beachrocks [36,37,51,52,59] may play a crucial role in the future of soft enginiering techniques.

Conclusions
Beachrocks are natural formations developed under specific physio-bio-chemical conditions. These formations are acting, in many cases, as breakwaters protecting the shore by the sea waves and their energy. Furthermore, they act as sediment traps if they are uplifted and stabilize the sandy beaches. In our case studies of (a) Diolkos, Greece and (b) Sumuide, Japan, we analyzed the beachrock formation mechanism by studying their natural characteristic and by simulating their formation using local bacteria and material. Diolkos beachrock was formed in the upper part of the intertidal zone, while it consisted of detrital material originating from the local bedrock. Sumuide beachrock was formed in the low intertidal-upper subtidal zone and it consisted of coral sand and foraminifera fragments. Their corresponding artificial samples showed similar physical and chemical results and properties The results from the artificial beachrocks showed that the bacteria Micrococcus yunnanensis sp. of Diolkos created a more consolidated sample, with better physical characteristics than the bacteria Pararhodobacter sp. from the Sumuide area. The Sumuide artificial beachrocks showed that the bacterial density greatly affects the solidification result. Different parameters in the experiments have shown that the understanding of bacteria ureolysis ability plays a more important role than the particle homogeneity. Furthermore, the artificial beachrocks showed inferior but similar properties with their corresponding natural beachrocks.
Concluding, the artificial beachrock experiment may lead to a new era of a sustainable soft engineering constructions where we can imitate the natural beachrock characteristic as a break water. By creating artificial beachrocks in vulnerable sandy beaches affected by erosion, we achieve ecofriendly coastal sustainability while (a) saving energy as this method does not needs large amounts of any type of energy, (b) reducing CO 2 emissions as this method does not involve cement, and (c) building materials due to the fact of using the local beach material. Finally, artificial beachrocks, by sharing the same natural principles as the natural occurrences, may have a positive impact on marine biodiversity as they will play the role of rocky habitats for fisheries, algae, microorganisms, etc.