Response of the Durum Wheat Cultivar Um Qais ( Triticum turgidum subsp. durum ) to Salinity

: The threat of land degradation by salinization in Jordan has been increasing over the last decades. Therefore, information about the response of local cultivars to salinity is needed to help farmers choose the most productive cultivars for areas with salt-a ﬀ ected soils. A recently released durum wheat cultivar Um Qais ( Triticum turgidum subsp. durum ) has shown to be productive under normal conditions but to date there are no known studies on its tolerance to salinity. Two experiments were conducted to investigate the response of Um Qais cultivar to salinity. A ﬁeld experiment was carried out in the Jordan Valley, which is known for its hot, dry climate during the summer and low rainfall and moderate temperature during the winter. Three water salinity levels (S): S1 (2 dS m − 1 ), S2 (4 dS m − 1 ), and S3 (8 dS m − 1 ) with three irrigation amounts (R) (control = 120% (R1), 100% (R2), and 70% (R3)) were used in the ﬁeld. A greenhouse experiment was conducted using four levels of saline water (S): S1 (0.65 dS m − 1 ), S2 (4 dS m − 1 ), S3 (8 dS m − 1 ), and S4 (10 dS m − 1 ). In both experiments, the leaf area index (LAI) and canopy height were measured during three growth stages, tillering, ﬂag leaf, and maturity. The number of grains, grain yield, and above-ground biomass were measured after harvesting while soil salinity and pH were measured every three weeks during the growing season. The results showed that the maximum reduction in yield was of the 28% in the ﬁeld experiment when the average soil salinity was of 6.8 ± 1.1 (standard error) dS m − 1 at the middle stages of the season. Signiﬁcant changes were shown in the treatments of the ﬁeld experiments for maximum LAI, number of grains, and aboveground biomass, but not for plant height. For the greenhouse treatments, about 60% of the maximum grain yield was obtained when the average soil salinity was 9.94 ± 1.89 dS m − 1 at the middle stage. Grain yield was the most sensitive parameter to the increase in soil salinity during the season. According to the ﬁndings of both experiments, Um Qais can be cultivated in moderately saline soils.


Introduction
Salinity in arable land is one of the major threats to crop production [1] and the risk of salinization increases as more land is needed to feed a growing world population [2]. The total global area of salt-affected soils was estimated to be approximately 830 million hectares (ha) in 2013 [3] and approximately 10 million ha are affected by salt accumulation each year [4]. This rate may be accelerated in the future as a result of climate change, excessive use of groundwater, increasing use of low-quality water in irrigation, and poor drainage [5]. It is estimated that half of the arable lands worldwide will be affected by salinity during the coming 30 years [6]. Many of the countries in the Mediterranean region experience salinity problems that affect their agricultural production, among them Jordan, Syria Agriculture 2019, 9,135 3 of 14 seed yield, harvest index, number of spikes per plant and number of kernels per spike. It also proved to be a more efficient cultivar because of its high productivity under improved growing conditions according to a study conducted in the southern region in Jordan in 2005 [22]. Omrabi 6 was certified as an improved durum wheat cultivar under the name Um Qais (Triticum turgidum subsp. durum) by the Ministry of Agriculture in Jordan [22]. So far only a few studies have been conducted in Jordan about the tolerance of localdurum wheat cultivars to salinity [23][24][25] and none included the recently released cultivar Um Qais durum wheat. Therefore, the objective of this study was to evaluate the rate of development and growth of Um Qais under saline conditions when irrigated with different amounts of water and salinity levels. The study was conducted in the central region of the Jordan Valley, where most of the soil is moderately saline and the irrigation water is relatively saline [26]. The field experiment was designed to be similar to the common management practices in the Jordan Valley's agricultural areas. The field experiment was supported by a greenhouse study to assess the response of Um Qais when irrigated with different water salinity levels in a well-controlled environment.

Materials and Methods
The Um Qais durum wheat cultivar was released in 2005 and is known for its high productivity when it is cultivated under normal non-saline conditions when compared to other local cultivars. Therefore, it is widely used by farmers in Jordan [22]. In order to understand the response of Um Qais to salinity, two experiments were conducted, one under field conditions and one in a greenhouse.

Field Experiment
The field experiment was conducted at the Agricultural Research Station in the central Jordan Valley (Damea) (32.08 N, 35.58 E) where soil salinity showed a pronounced increase from 2007 to 2013 ranging between 51% and 63% [22]. The soil type is Entisol with a fine loamy sand texture. The soil depth in the study area is about 1.5 m and the land has no drainage system. The experimental design was split plot randomized complete block design and the experimental area was 456.5 m 2 , which was divided into four blocks. Each block contained three main plots to which the three irrigation levels (R) were assigned: R1 (120%), R2 (100%), and R3 (70%). Each main plot was split into three subplots to which three water salinity levels (S) were assigned: S1 (2 dS m −1 ), S2 (4 dS m −1 ) and S3 (8 dS m −1 ) for a total of nine treatments (S1R1, S1R2, S1R3, S2R1, S2R2, S2R3, S3R1, S3R2 and S3R3) and 36 plots measuring 3 × 3 m. The design was based on the hypothesis that different levels of irrigation amounts would affect the amount of salt accumulation in the root zone, especially in arid and semi-arid regions where the amount of rainfall is low. The salt accumulation was expected to be in the most stressed treatments of high salinity and low irrigation amounts, thus the reduction in yield and growth was expected to be significant in the most stressed treatments. The Um Qais durum wheat cultivar was planted on 29 December 2017 with a planting density of 200 plant per m 2 and seven rows in each plot of 3 m and 40 cm plot length and row spacing, respectively. Urea fertilizer was mixed with irrigation water that was applied on 29 December 2017, 15 February 2018, and 15 March 2018 with 200, 100, and 100 kg ha −1 , respectively. A total of 250 mL m −3 of 2,4-D (weed killer) was added once on 3 March 2018. A drip irrigation system was used in the field, which was adequately watered during the first irrigation application. Salinity and irrigation treatments were initiated at the second irrigation within initial stages. Soil water content was determined weekly using the gravimetric method at the beginning and by Time Domain Reflectometry (TDR) PICO-BT IMKO type (IMKO Micromodultechnik GmbH, Ettlingen, Germany). The TDR soil moisture measurements were taken at soil depths of 10 and Salinity levels were determined according to the salinity threshold described by Maas and Hoffman [27] and the levels of water salinity that are internationally used for durum wheat salt experiments [28,29]. The irrigation amounts were determined according to a study conducted by Prathapar and Qureshi [30], which showed that generally during one growing season plants start to be affected by water stress when they are irrigated with 80% readily available water (RAW). The control in the field was the treatment S1R1 with the lowest irrigation water salinity (2 dS m −1 ) and the highest irrigation water amount (120% RAW). The control was expected to prevent any possible accumulation of salt in the soil during the season, thereby allowing the cultivar to grow without any salinity stress. All other management practices except salinity and irrigation levels were the same for all treatments in the field.
The RAW is defined as the soil moisture held between field capacity (FC) and a nominated refill point for unrestricted growth, at which water can be easily absorbed by plants from the soil. Irrigation water was applied when the measured soil moisture reached the management allowable depletion (MAD), which is defined as the maximum amount of Plant Available Water (PAW) allowed to be removed from the soil before irrigation refill occurs. The MAD for this experiment was assumed to be 0.5 based on Allen's [31] recommendation for durum wheat. RAW was calculated by first calculating the critical soil moisture which is defined as the threshold level of soil water content in mm above which the irrigation water should be applied: The Critical soil moisture = MAD FC PWP z, Where (FC) is field capacity, (PWP) is permanent wilting point, and z is rooting depth in mm. RAW is calculated as the difference between (FC) and the critical soil moisture as follows: Some soil chemical and physical properties of each block were measured first on 20 December 2017 at depths of 10 and 30 cm to determine the initial conditions prior to planting. Soil texture, field capacity, permanent wilting point and bulk density were measured using the pipette [32], ceramic plate [33] and core method [34]. A pH and EC meters of type BP3001 were used to measure soil salinity and pH every three weeks taking four soil samples at four depths (10,30,50, and 70 cm) of the 18 plots at the two central blocks. The saturated soil extract method was used to measure the soil electrical conductivity (ECe) [35]. Other ions in the soil were measured for the first 30 cm depth at the initial conditions before planting, except Na, Ca, and Mg were measured twice before planting and after harvesting at 30 cm depth K and Na concentrations were analyzed using a flame photometer (Jenway Research PFP7/C, Cole-parmer Inc., Eaton Socon, England). The cation exchange capacity was determined using the sodium acetate method [36]. Other ions, including Cl, Ca, Mg and HCO3, were measured using titration according to the methods illustrated by Bower [37]. Phosphorus was determined using the Olsen method [38], and total Nitrogen was determined with the Kjeldahl Salinity levels were determined according to the salinity threshold described by Maas and Hoffman [27] and the levels of water salinity that are internationally used for durum wheat salt experiments [28,29]. The irrigation amounts were determined according to a study conducted by Prathapar and Qureshi [30], which showed that generally during one growing season plants start to be affected by water stress when they are irrigated with 80% readily available water (RAW). The control in the field was the treatment S1R1 with the lowest irrigation water salinity (2 dS m −1 ) and the highest irrigation water amount (120% RAW). The control was expected to prevent any possible accumulation of salt in the soil during the season, thereby allowing the cultivar to grow without any salinity stress. All other management practices except salinity and irrigation levels were the same for all treatments in the field.
The RAW is defined as the soil moisture held between field capacity (FC) and a nominated refill point for unrestricted growth, at which water can be easily absorbed by plants from the soil. Irrigation water was applied when the measured soil moisture reached the management allowable depletion (MAD), which is defined as the maximum amount of Plant Available Water (PAW) allowed to be removed from the soil before irrigation refill occurs. The MAD for this experiment was assumed to be 0.5 based on Allen's [31] recommendation for durum wheat. RAW was calculated by first calculating the critical soil moisture which is defined as the threshold level of soil water content in mm above which the irrigation water should be applied: where (FC) is field capacity, (PWP) is permanent wilting point, and z is rooting depth in mm. RAW is calculated as the difference between (FC) and the critical soil moisture as follows: Some soil chemical and physical properties of each block were measured first on 20 December 2017 at depths of 10 and 30 cm to determine the initial conditions prior to planting. Soil texture, field capacity, permanent wilting point and bulk density were measured using the pipette [32], ceramic plate [33] and core method [34]. A pH and EC meters of type BP3001 were used to measure soil salinity and pH every three weeks taking four soil samples at four depths (10,30,50, and 70 cm) of the 18 plots at the two central blocks. The saturated soil extract method was used to measure the soil electrical conductivity (ECe) [35]. Other ions in the soil were measured for the first 30 cm depth at the initial conditions before planting, except Na, Ca, and Mg were measured twice before planting and after harvesting at 30 cm depth K and Na concentrations were analyzed using a flame photometer (Jenway Research PFP7/C, Cole-parmer Inc., Eaton Socon, England). The cation exchange Agriculture 2019, 9, 135 5 of 14 capacity was determined using the sodium acetate method [36]. Other ions, including Cl, Ca, Mg and HCO 3 , were measured using titration according to the methods illustrated by Bower [37]. Phosphorus was determined using the Olsen method [38], and total Nitrogen was determined with the Kjeldahl method [39]. Sodium Adsorption Ratio (SAR) was calculated from the measured Na, Ca and Mg in the soil water extract as follows: The electrical conductivity was 4 dS m −1 , pH was 8, and Na and Cl were 41.98 and 0.33 ppm, respectively. The bulk density was 1.58 g cm −3 , and the soil texture was loamy sand. Soil chemical and physical properties are shown in Table 1. The total amount of the applied irrigation water for the whole field experiment was 90.18 m 3 and was distributed as 37.8 (350 mm), 30.78 (250 mm) and 21.6 (200 mm) m 3 for R1, R2, and R3 respectively. NaCl was added to each tank at the rate of 1.6 kgm −3 for the S2 experiments and 3.5 kgm −3 for the S3 treatments. The irrigation water of the control (2 dS m −1 ) plot was the same as the water that is used by farmers in the Jordan Valley, without any change to its salinity. The field experiment was harvested on 2 April 2018.

Greenhouse Experiment
The second experiment was conducted in a greenhouse at the University of Jordan. The same durum wheat cultivar and soil type were used. Sixteen identical pots (80 l) with rectangular dimensions of 40 × 40 cm and 50 cm deep were planted on 30 December 2017 and were harvested on 10 Aprril 2018. A randomized complete block design (RBD) with four replicates for each treatment was used in the experiment. The four salinity levels of the experiment were 0.68, 4, 8, and 10 dS m −1 for S1 (the control), S2, S3, and S4, respectively. The design based on the hypothesis that pots in greenhouse could accumulate salts and significantly suppress the yield and growth parameters without deficit irrigation. A higher level of saline irrigation water was used in greenhouse where a further reduction in growth and development was expected. Soil samples for ECe and pH were taken every three weeks during the growing season at depths of 10 and 30 cm.
In each pot, six seeds were planted in two rows. Soil water content was monitored on the same dates as in the field experiment by gravimetric soil moisture and tensiometers that were installed in the 16 pots at a depth of 20 cm from the soil surface ( Figure 2). The treatments were irrigated by saline water after emergence and irrigation was conducted using the same procedure as in the field experiment. Pots were irrigated at 100% RAW, and each time the soil water content reached the critical level, irrigation water was applied to replenish soil water content to FC. without deficit irrigation. A higher level of saline irrigation water was used in greenhouse where a further reduction in growth and development was expected. Soil samples for ECe and pH were taken every three weeks during the growing season at depths of 10 and 30 cm.
In each pot, six seeds were planted in two rows. Soil water content was monitored on the same dates as in the field experiment by gravimetric soil moisture and tensiometers that were installed in the 16 pots at a depth of 20 cm from the soil surface ( Figure 2). The treatments were irrigated by saline water after emergence and irrigation was conducted using the same procedure as in the field experiment. Pots were irrigated at 100% RAW, and each time the soil water content reached the critical level, irrigation water was applied to replenish soil water content to FC. A micro weather station was installed inside the greenhouse, consisting of a pyranometer (PYR) for daily solar radiation, a thermistor for hourly air temperature and a humidity sensor. Canopy height and LAI were measured during tillering, flag leaf, and maturity development stages. The measurements were taken for all plants in each pot.
The total applied irrigation water in the greenhouse experiment was 0.973 m 3 for each pot. NaCl was added for the three treatments S2, S3 and S4 in the amounts of 1.75, 3.5 and 4.5 g l −1 , respectively. The water used in the control was tap water and no NaCl was added to irrigation water for the control treatment. The salinity of the tap water was approximately 0.68 dS m −1 . The electrical conductivity of irrigation water (ECiw) was measured frequently before and after irrigation. The mass of NaCl that was needed to reach the salinity levels in irrigation water was estimated for both experiments using the procedure described by Rani and Sharma [40] where NaCl was used as solute in g l −1 . A relationship was found that amount of sodium chloride required in terms of gram per liter is half of the desired EC in dS m −1 with some adjustments to get the final ECiw. Table 2 shows the chemical properties of the irrigation water that was used in the control treatment for the two experiments. For the field experiment, the electrical conductivity of irrigation water (ECiw) was 2 ± 0.31 dS m −1 , the pH and sodium adsorption ratio (SAR) were 7.8 ± 0.04 and 5.22 ± 0.04, respectively, and the concentration of Na was an average of 240 ± 0.91 ppm. For the greenhouse experiment, the pH and SAR were 7.53 ± 0.14 and 5.230 ± 0.05, respectively. The Na concentration in the control was 170 ± 0.57 ppm. A micro weather station was installed inside the greenhouse, consisting of a pyranometer (PYR) for daily solar radiation, a thermistor for hourly air temperature and a humidity sensor. Canopy height and LAI were measured during tillering, flag leaf, and maturity development stages. The measurements were taken for all plants in each pot.
The total applied irrigation water in the greenhouse experiment was 0.973 m 3 for each pot. NaCl was added for the three treatments S2, S3 and S4 in the amounts of 1.75, 3.5 and 4.5 g l −1 , respectively. The water used in the control was tap water and no NaCl was added to irrigation water for the control treatment. The salinity of the tap water was approximately 0.68 dS m −1 . The electrical conductivity of irrigation water (ECiw) was measured frequently before and after irrigation. The mass of NaCl that was needed to reach the salinity levels in irrigation water was estimated for both experiments using the procedure described by Rani and Sharma [40] where NaCl was used as solute in g l −1 . A relationship was found that amount of sodium chloride required in terms of gram per liter is half of the desired EC in dS m −1 with some adjustments to get the final ECiw. Table 2 shows the chemical properties of the irrigation water that was used in the control treatment for the two experiments. For the field experiment, the electrical conductivity of irrigation water (EC iw ) was 2 ± 0.31 dS m −1 , the pH and sodium adsorption ratio (SAR) were 7.8 ± 0.04 and 5.22 ± 0.04, respectively, and the concentration of Na was an average of 240 ± 0.91 ppm. For the greenhouse experiment, the pH and SAR were 7.53 ± 0.14 and 5.230 ± 0.05, respectively. The Na concentration in the control was 170 ± 0.57 ppm.

Experimental Measurements
Canopy height, LAI, aboveground biomass, grain number per unit area, grain yield and duration of growth stages were measured for the filed and greenhouse experiments. The duration of the three development stages, tillering, flag leaf and maturity were recorded using days after planting (DAP), which is defined as the days needed to reach the growth stage from the planting date. Leaf area index (LAI) was measured using a ceptometer (AccuPAR model LP-80, Decagon Devices, Inc., Pullman, WA, USA), and a graduated rod was used to measure canopy height at tillering, flag leaf, and maturity. The number of grains per unit area, grain yield and aboveground biomass were measured at harvest using all plants in 1 m 2 for each plot in the field. In the greenhouse, all plants in each pot were measured after harvesting.

Statistical Analysis
ANOVA and t-test were applied using the general linear model (GLM) procedure of the Statistical Analysis System (NCSS Statistical Software, version 12, Kaysville, UT, USA). Differences were considered significant at α = 0.05.

Results
The relationship between salinity levels and irrigation amounts in the field experiment is illustrated in Figure 3. The average soil salinity at the end of the season increased slightly for the S1 treatment (control) from an average of 1.59 ± 0.18 dS m −1 for R1 (120% RAW) to an average of 2.72 ± 0.33 dS m −1 (S1) for R3 (70% RAW), while soil salinity for the S2 treatment increased gradually from 2.67 ± 0.42, 3.41 ± 0.49 to 3.73 ± 0.59 dS m −1 for (120% RAW), (100% RAW), and (70% RAW) for R1, R2, and R3, respectively. The increase was most noticeable for the S3 treatment, where the average changed in the range of 4.67 ± 0.64, 6.31 ± 05, and 6.05 ± 0.71 dS m −1 for R1, R2 and R3, respectively. The highest soil salinity levels for treatments S1, S2 and S3 were observed for R3 (70% RAW).

Experimental Measurements
Canopy height, LAI, aboveground biomass, grain number per unit area, grain yield and duration of growth stages were measured for the filed and greenhouse experiments. The duration of the three development stages, tillering, flag leaf and maturity were recorded using days after planting (DAP), which is defined as the days needed to reach the growth stage from the planting date. Leaf area index (LAI) was measured using a ceptometer (AccuPAR model LP-80, Decagon Devices, Inc., Pullman, WA, USA), and a graduated rod was used to measure canopy height at tillering, flag leaf, and maturity. The number of grains per unit area, grain yield and aboveground biomass were measured at harvest using all plants in 1 m 2 for each plot in the field. In the greenhouse, all plants in each pot were measured after harvesting.

Statistical Analysis
ANOVA and t-test were applied using the general linear model (GLM) procedure of the Statistical Analysis System (NCSS Statistical Software, version 12, Kaysville, UT, USA). Differences were considered significant at α = 0.05.

Results
The relationship between salinity levels and irrigation amounts in the field experiment is illustrated in Figure 3. The average soil salinity at the end of the season increased slightly for the S1 treatment (control) from an average of 1.59 ± 0.18 dS m −1 for R1 (120% RAW) to an average of 2.72 ± 0.33 dS m −1 (S1) for R3 (70% RAW), while soil salinity for the S2 treatment increased gradually from 2.67 ± 0.42, 3.41 ± 0.49 to 3.73 ± 0.59 dS m −1 for (120% RAW), (100% RAW), and (70% RAW) for R1, R2, and R3, respectively. The increase was most noticeable for the S3 treatment, where the average changed in the range of 4.67 ± 0.64, 6.31 ± 05, and 6.05 ± 0.71 dS m −1 for R1, R2 and R3, respectively. The highest soil salinity levels for treatments S1, S2 and S3 were observed for R3 (70% RAW). . Mean soil salinity (ECe) at the end of the growing season in the field experiment for the three irrigation water salinity levels (S1, S2, and S3) with the three irrigation levels (R1, R2, and R3) which was determined by RAW (Readily Available Water). Soil salinity increased gradually during the growing season for both experiments; the average ECe with the standard error for each treatment is shown in Figure 2. For the field experiment, the highest significant changes in soil salinity between from pre-planting to after-harvesting were for the S3R2 and S3R3 treatments (Figure 4a). An average soil salinity difference between ECe in during the growing season for S3R2 and S3R3 were 6.64 ± 0.25 and 6.1 ± 1.21 dS m −1 , respectively. The other treatments did not show any significant increase in soil salinity during the growing season. Soil salinity increased gradually during the growing season for both experiments; the average ECe with the standard error for each treatment is shown in Figure 2. For the field experiment, the highest significant changes in soil salinity between from pre-planting to after-harvesting were for the S3R2 and S3R3 treatments (Figure 4a). An average soil salinity difference between ECe in during the growing season for S3R2 and S3R3 were 6.64 ± 0.25 and 6.1 ± 1.21 dS m −1 , respectively. The other treatments did not show any significant increase in soil salinity during the growing season.
For the greenhouse experiment, the average soil salinity for S1 at the early stage pre-planting was 2.31 ± 0.05 dS m −1 reaching 2.87 ± 0.08 dS m −1 after-harvesting (Figure 4b). For S2 the early average salinity level was 2.30 ± 0.13 dS m −1 and 5.25 ± 0.28 dS m −1 after-harvesting. Both S3 and S4 had significant average soil salinity differences during the whole season that were approximately 8.45 ± 1.05 and 10.07 ± 2.25 dS m −1 , respectively.  For the greenhouse experiment, the average soil salinity for S1 at the early stage pre-planting was 2.31 ± 0.05 dS m −1 reaching 2.87 ± 0.08 dS m −1 after-harvesting (Figure 4b). For S2 the early average salinity level was 2.30 ± 0.13 dS m −1 and 5.25 ± 0.28 dS m −1 after-harvesting. Both S3 and S4 had significant average soil salinity differences during the whole season that were approximately 8.45 ± 1.05 and 10.07 ± 2.25 dS m −1 , respectively.
The durations of tillering, flag leaf and maturity development stages in the field experiment were the same for all field treatments (Table 3a). The LAI for the field experiment was significantly different for only the treatments S2R3, S3R1, S3R2 and S3R3 at the flag leaf development stage. During the For treatments S2, S3 and S4, grain yield was significantly different from the control (Table 4b).
A two-way ANOVA was calculated for the field experiment considering the different salinity levels (S1, S2, and S3) and irrigation amounts (R1, R2, and R3). The significant effect of the two factors on soil salinity is illustrated by the Fisher test (F) and probability (P). The irrigation water salinity (S) showed a significant impact on soil salinity, while irrigation levels were insignificant, and the interaction between the two factors in the field experiment was weak (Table 5a). Grain yield showed the most significant difference in response to salinity treatments for the field experiment (the lowest p-value). F critical (F crit) is called also F statistics that is used to determine the significant results when it is lower than F value (F). All parameters in the field had significant variations except plant height (Table 5a). For the greenhouse experiment, only grain yield and plant height had significant differences compared to the control, while the number of grains and aboveground biomass were not significantly different (Table 5b).

Discussion
The accumulation of sodium chloride in the soil in both the field and greenhouse experiments can be attributed to several causes. It was likely caused by multiple possibilities, primarily the low quality of irrigation water and the lack of sufficient irrigation water to reduce salt leaching, thereby resulting in further salt accumulation. The rate of soil salinization during the growing season was faster in all treatments in the greenhouse than in the field experiment (Figure 2), mainly due to the frequent irrigation of pots in the greenhouse compared to the field experiment. As a result, there was an unusually higher water uptake for the greenhouse pots than the irrigated fields when expressed in mm per unit area [41]. The accumulation of salts in the field was slow even for the most stressed treatment (S3R3), which can be explained by the short growing season in the field due to the hot climate in the Jordan Valley. The short growing season and respective limited amount of irrigation coupled with the loamy sand soil texture likely reduced the accumulation of saline in the soil as compared to accumulations reported in clay soil [41].
A comparison of the two experiments found no significant changes in the duration of the three development stages (Table 3). According to Grieve [42], moderate soil salinity does not have a noticeable effect on the timing of development stages for durum wheat, and that even high soil salinity level did not affect the duration of the vegetative phase, but does shorten the reproductive phase of durum wheat. The duration of the growing season in the greenhouse was higher than in the field (Table 3). Due to the unique conditions in the Jordan Valley 240 m below sea level, the average minimum field temperature was nearly 4 ºC higher than in the greenhouse. This higher temperature might be the reason for the short growing season in the field, as described by Barlow [43].
Results of the ANOVA for the parameters of both experiments (Table 5) show statistically significant differences for all the growth and development measurements for the field experiment except for plant height. On the other hand, grain yield and plant height were the only parameters that were significantly affected by the salinity increase in the greenhouse experiment (Table 5).
Although the soil at the beginning of the season was non-saline at germination and initial vegetative development, a reduction in the yield and yield components with the increase in soil salinity was observed. These finding suggests that the middle and late stages could be affected by soil salinity even when early stages were non-saline. Both experiments indicated that the most susceptible parameter to soil salinity was grain yield, similar to the finding of Singh and Chatrath [44], Steppuhn et al. [45], Saqib et al. [46] and Dikgwatlhe et al. [47]. The reduction in plant height with increasing soil salinity was significant for all salinity treatments in the greenhouse, where an accumulation of salts occurred during the earlier stages. This indicates that plant height is more sensitive to soil salinity when it is exposed to salinity at earlier stages, in accordance with the findings of Dikgwatlhe et al. [46].
Based on the grain yield data measured in the greenhouse experiment, the durum wheat cultivar Um Qais can be classified as a moderately salt tolerant cultivar. This is based on its relative yield when using saline irrigation water, according to the salt tolerance ranking criteria that were established by El-Hendawy [28] for durum wheat. Triticum turgidum subsp. Durum was considered relatively tolerant to Na and Cl ions compared to other durum wheat genotypes [29], in spite of the wide variation among the durum cultivars in tolerance to salinity [29]. The level of salt tolerance in durum wheat is highly related to the ability of the genotype to conserve high K to Na ratio in the shoot [48]. There was a relatively high reduction in yield of 40% for the S4 treatment in the greenhouse compared to the control, and the decline in yield for the S4 treatment compared to S3 was greater than 15%. Although it is very difficult to determine at which time during the growing season the effect of ion toxicity occurs, a study was conducted by Dura [25] to investigate the salt tolerance of three durum wheat cultivars in Jordan. One of these cultivars was Omrabi5 which is very similar to Um Qais. The response to NaCl toxicity was also investigated at different concentrations. Dura indicated that when the Na concentration in tissues reached 100 mM, it may inhibit the function of the most enzymes. Dura also implied that the effect of Na toxicity was observed at 100 mM (≈ 9 dS m −1 ) NaCl irrigation water. Accordingly, S4 treatment in the greenhouse experiment with 10 dS m −1 irrigation water salinity was expected to be affected by sodium ion toxicity.

Conclusions
In both the field and greenhouse experiments, Um Qais proved to be a moderately resistant crop to soil salinity. Grain yield was the most affected parameter to soil salinity during the middle and late stages of plant development. The maximum reduction of yield for Um Qais cultivar was 40% in the greenhouse when it was irrigated with 10 dS m −1 NaCl. The findings of this study are a good indication of the ability of Um Qais durum wheat to be productive in moderately saline soils and its use is recommended for cultivation in the Jordan Valley. However, more studies are needed to compare this cultivar with other local cultivars and to test their productivity in other areas with saline soils in Jordan and similar regions across the globe.
Author Contributions: L.H. is the main researcher who developed the project proposal, experimental design, data collection, analysis, and main write up; A.S. is the main supervisor of L.H. and who conceptualized the experiment, methodology, review, and editing; G.H. is the co-supervisor who contributed to the conceptualization, review and editing; V.S. also contributed to conceptualization, methodology, review and editing.
Funding: This research received funding from the University of Jordan with no external funding.