Genetic Engineering of Energy Crops to Reduce Recalcitrance and Enhance Biomass Digestibility

Bioenergy, biofuels, and a range of valuable chemicals may be extracted from the abundantly available lignocellulosic biomass. To reduce the recalcitrance imposed by the complex cell wall structure, genetic engineering has been proposed over the years as a suitable solution to modify the genes, thereby, controlling the overall phenotypic expression. The present review provides a brief description of the plant cell wall structure and its compositional array i.e., lignin, cellulose, hemicellulose, wall proteins, and pectin, along with their effect on biomass digestibility. Also, this review discusses the potential to increase biomass by gene modification. Furthermore, the review highlights the potential genes associated with the regulation of cell wall structure, which can be targeted for achieving energy crops with desired phenotypes. These genetic approaches provide a robust and assured method to bring about the desired modifications in cell wall structure, composition, and characteristics. Ultimately, these genetic modifications pave the way for achieving enhanced biomass yield and enzymatic digestibility of energy crops, which is crucial for maximizing the outcomes of energy crop breeding and biorefinery applications.


Introduction
Biomass has the potential to provide a renewable form of energy by going through engineered processes, such as anaerobic digestion, fermentation, and gasification [1].A group of researchers contemplated that, to reduce the overall CO 2 to half, renewable energy from biological sources must be squared two times by 2050.Lignocellulosic biomass is a plentiful resource for bioenergy, with an estimated productivity of 1.5 × 10 10 tons per year and a conversion efficiency of about 0.6 [2].However, the robust nature of lignocellulosic biomass makes the bioconversion process quite expensive due to the necessity of carrying out pretreatment.The structure of cell wall polymers, and its inability to be converted easily, makes room for genetic modifications [3].The modifications caused by genetic manipulations also transferred to the upcoming generations, unlike pretreatment methods, which need to be carried out each time.Therefore, genetic modifications seem to be a one-time investment for bringing about the desired changes in bioenergy crops for the long term [4].
Cellulose, hemicelluloses, lignin, and pectins are the key constituents of a plant cell wall complex (Figure 1).The plant cell wall consists of various complicated polymer structures and has multidimensional functions.In this regard, genetic amendment of the plant cell wall complex is sometimes related to imperfections in the growth, health, and overall change of the plant cell wall lifespan, considerably affecting the agronomic characters of crops [5].So, to increase biomass yield by genetic modification, a plant's basic functional cell wall structure for its growth should be taken care, in parallel [3].Biomass yield can be estimated in terms of the growth of the plant and its parts i.e., height of plant, leaflet size, shoot length, etc. [6][7][8].The changes in height or size of the leaflet or shoot are measured by comparing the growth with wild plants without genetic modifications [6][7][8].
sometimes related to imperfections in the growth, health, and overall change of the plant cell wall lifespan, considerably affecting the agronomic characters of crops [5].So, to increase biomass yield by genetic modification, a plant's basic functional cell wall structure for its growth should be taken care, in parallel [3].Biomass yield can be estimated in terms of the growth of the plant and its parts i.e., height of plant, leaflet size, shoot length, etc. [6][7][8].The changes in height or size of the leaflet or shoot are measured by comparing the growth with wild plants without genetic modifications [6][7][8].

Biomass Bioconversion and the Plant Cell Wall Complex
The plant cell complex varies throughout the plant kingdom and, hence, it remains demanding to select polymers on an individual level that influence the enzymatic debasement of feedstock.Cellulose, hemicelluloses, and lignin are the main polymer structures for the identification and characterization of biomass and its bioconversion.In the plant cell wall, cellulose is a high density polymer consisting of β-1,4-glucan chains (Figure 2).Cellulose shows a semi-crystalline and fibrous structure because of hydrogen bonding (inter and intramolecular) between β-1,4-glucan forming chains [9].In the innate state, cellulose molecules are held together by intermolecular hydrogen bonds, but the strong tendency of cellulose to form intramolecular and intermolecular hydrogen bonds increases the rigidity of cellulose.This also makes cellulose highly insoluble and highly resistant to most organic solvents.Cellulose consists of (1,4)-D-glucopyranose units, which are attached by β-1,4 linkages, with an average molecular weight of around 100,000 [9].Interaction of cellulose with other polymers present in the plant cell wall depends on this fibrous and mechanically strong nature of cellulose crystallinity [10].For determining crystallinity in agricultural biomass and energy crops experimentally, X-ray diffraction (XRD) is, in general, employed and results are expressed as the crystallinity index (CrI).This factor, CrI, has been reported for affecting the lodging resistance of rice in a negative manner, a trait which determines the yield of crops [5].

Biomass Bioconversion and the Plant Cell Wall Complex
The plant cell complex varies throughout the plant kingdom and, hence, it remains demanding to select polymers on an individual level that influence the enzymatic debasement of feedstock.Cellulose, hemicelluloses, and lignin are the main polymer structures for the identification and characterization of biomass and its bioconversion.In the plant cell wall, cellulose is a high density polymer consisting of β-1,4-glucan chains (Figure 2).Cellulose shows a semi-crystalline and fibrous structure because of hydrogen bonding (inter and intramolecular) between β-1,4-glucan forming chains [9].In the innate state, cellulose molecules are held together by intermolecular hydrogen bonds, but the strong tendency of cellulose to form intramolecular and intermolecular hydrogen bonds increases the rigidity of cellulose.This also makes cellulose highly insoluble and highly resistant to most organic solvents.Cellulose consists of (1,4)-D-glucopyranose units, which are attached by β-1,4 linkages, with an average molecular weight of around 100,000 [9].Interaction of cellulose with other polymers present in the plant cell wall depends on this fibrous and mechanically strong nature of cellulose crystallinity [10].For determining crystallinity in agricultural biomass and energy crops experimentally, X-ray diffraction (XRD) is, in general, employed and results are expressed as the crystallinity index (CrI).This factor, CrI, has been reported for affecting the lodging resistance of rice in a negative manner, a trait which determines the yield of crops [5].On the other hand, hemicellulose has a dissimilar composition based on plant species.The hemicellulose backbone can be a homopolymer or heteropolymer, and consists of five carbon and six carbon sugars i.e., Xylose in hardwoods and mannose in softwood, with side chains of acetyl, gluconoryl, or arabinofuranosyl units (Figure 3) [11].Xylans, mannans, and glucans can form backbones, with xylans and mannans being the most common [1].Galactans, arabinan, and arabinanogalactans are the main sugar monomers in the hemicellulose, but these monomers do not share an equatorial β-1,4 linked backbone structure.Glucuronoxylan is the prevalent component in hardwood.Hemicellulose connects cellulose and lignin with cross linkages formed by covalent and hydrogen bonds.These linkages strengthen the plant cell wall and have a major role in the recalcitrance behavior of these polymers [1].Apart from cellulose and hemicellulose, lignin is also an important part of the plant cell wall, having complex amorphous polymers of coniferyl sinapyl and coumaryl alcohol.So, as these three components of lignocellulosic biomass are cross linked, they form a sturdy 3-D matrix, which acts as a barrier for enzymatic activity during anaerobic digestion of biomass for biogas production [1].Lignin acquires around 10-25% of the lignocellulosic biomass by molecular weight (Table 1).It works as a glue by filling the gap between cellulose and hemicellulose.It contains a large polymer of phenyl-propane, methoxy group, and non-carbohydrate polyphenolic constituents [9].These constituents bind the cell wall components and phenyl propane works as a main block of lignin.Phenyl-propanes are denoted as 0, I, and II methoxy groups, which are attached to rings and give the special structure of I, II, and III [11].Structure I exists in plants (grasses), structure II exists in wood (conifers), and structure III is found in deciduous wood.Coniferyl alcohol, sinapyl alcohol, and p-coumaryl alcohol are three monomers of lignin, as shown in Figure 4. On the other hand, hemicellulose has a dissimilar composition based on plant species.The hemicellulose backbone can be a homopolymer or heteropolymer, and consists of five carbon and six carbon sugars i.e., Xylose in hardwoods and mannose in softwood, with side chains of acetyl, gluconoryl, or arabinofuranosyl units (Figure 3) [11].Xylans, mannans, and glucans can form backbones, with xylans and mannans being the most common [1].Galactans, arabinan, and arabinanogalactans are the main sugar monomers in the hemicellulose, but these monomers do not share an equatorial β-1,4 linked backbone structure.Glucuronoxylan is the prevalent component in hardwood.On the other hand, hemicellulose has a dissimilar composition based on plant species.The hemicellulose backbone can be a homopolymer or heteropolymer, and consists of five carbon and six carbon sugars i.e., Xylose in hardwoods and mannose in softwood, with side chains of acetyl, gluconoryl, or arabinofuranosyl units (Figure 3) [11].Xylans, mannans, and glucans can form backbones, with xylans and mannans being the most common [1].Galactans, arabinan, and arabinanogalactans are the main sugar monomers in the hemicellulose, but these monomers do not share an equatorial β-1,4 linked backbone structure.Glucuronoxylan is the prevalent component in hardwood.Hemicellulose connects cellulose and lignin with cross linkages formed by covalent and hydrogen bonds.These linkages strengthen the plant cell wall and have a major role in the recalcitrance behavior of these polymers [1].Apart from cellulose and hemicellulose, lignin is also an important part of the plant cell wall, having complex amorphous polymers of coniferyl sinapyl and coumaryl alcohol.So, as these three components of lignocellulosic biomass are cross linked, they form a sturdy 3-D matrix, which acts as a barrier for enzymatic activity during anaerobic digestion of biomass for biogas production [1].Lignin acquires around 10-25% of the lignocellulosic biomass by molecular weight (Table 1).It works as a glue by filling the gap between cellulose and hemicellulose.It contains a large polymer of phenyl-propane, methoxy group, and non-carbohydrate polyphenolic constituents [9].These constituents bind the cell wall components and phenyl propane works as a main block of lignin.Phenyl-propanes are denoted as 0, I, and II methoxy groups, which are attached to rings and give the special structure of I, II, and III [11].Structure I exists in plants (grasses), structure II exists in wood (conifers), and structure III is found in deciduous wood.Coniferyl alcohol, sinapyl alcohol, and p-coumaryl alcohol are three monomers of lignin, as shown in Figure 4. Hemicellulose connects cellulose and lignin with cross linkages formed by covalent and hydrogen bonds.These linkages strengthen the plant cell wall and have a major role in the recalcitrance behavior of these polymers [1].Apart from cellulose and hemicellulose, lignin is also an important part of the plant cell wall, having complex amorphous polymers of coniferyl sinapyl and coumaryl alcohol.So, as these three components of lignocellulosic biomass are cross linked, they form a sturdy 3-D matrix, which acts as a barrier for enzymatic activity during anaerobic digestion of biomass for biogas production [1].Lignin acquires around 10-25% of the lignocellulosic biomass by molecular weight (Table 1).It works as a glue by filling the gap between cellulose and hemicellulose.It contains a large polymer of phenyl-propane, methoxy group, and non-carbohydrate polyphenolic constituents [9].These constituents bind the cell wall components and phenyl propane works as a main block of lignin.Phenyl-propanes are denoted as 0, I, and II methoxy groups, which are attached to rings and give the special structure of I, II, and III [11].Structure I exists in plants (grasses), structure II exists in wood (conifers), and structure III is found in deciduous wood.Coniferyl alcohol, sinapyl alcohol, and p-coumaryl alcohol are three monomers of lignin, as shown in Figure 4.Both cellulose and hemicelluloses are biodegradable in an independent form, but in the lignocellulosic substrate they are encrusted by lignin.The crosslinks present between cellulose, hemicelluloses, and lignin polymer forms a physical barrier that makes them inaccessible for enzymatic hydrolysis during anaerobic digestion.Furthermore, the ratio of coniferyl to syringyl monomers in lignin is also a crucial factor to determine the degree of biomass recalcitrance [4].Identification of key genes involved in lignin biosynthesis could be one of the best strategies towards genetic modification of lignins and esterified phenolic acids in biomass [4].

Genetic Modification in the Plant Cell Wall
To obtain a high biomass yield, the primary approach adopted by genetic engineers is to improve the polymer synthesis in the cell wall, specifically, targeting cellulose.However, the challenge is to maintain the inherent characteristics of the plant cell wall, which is primarily inclined towards providing mechanical strength and protection to plants rather than providing a biomass yield.Excess compromising with the polymers accounting for these protective features will create interference with optimal plant growth.Therefore, identification of appropriate genes for genetic transformation and mutagenesis is a vital step, along with employing effective promoters [13][14][15][16] A plethora of genes, in the count of thousands, are reported to participate in the process of cell wall polymer synthesis, modification, and degradation [17].Several mutant plants showed improved growth yield and biomass degradation after genetic modifications when compared to the wild type (Figures 5 and 6).The genetic engineering approaches based on these genes have been summarized in the following section.Both cellulose and hemicelluloses are biodegradable in an independent form, but in the lignocellulosic substrate they are encrusted by lignin.The crosslinks present between cellulose, hemicelluloses, and lignin polymer forms a physical barrier that makes them inaccessible for enzymatic hydrolysis during anaerobic digestion.Furthermore, the ratio of coniferyl to syringyl monomers in lignin is also a crucial factor to determine the degree of biomass recalcitrance [4].Identification of key genes involved in lignin biosynthesis could be one of the best strategies towards genetic modification of lignins and esterified phenolic acids in biomass [4].

Genetic Modification in the Plant Cell Wall
To obtain a high biomass yield, the primary approach adopted by genetic engineers is to improve the polymer synthesis in the cell wall, specifically, targeting cellulose.However, the challenge is to maintain the inherent characteristics of the plant cell wall, which is primarily inclined towards providing mechanical strength and protection to plants rather than providing a biomass yield.Excess compromising with the polymers accounting for these protective features will create interference with optimal plant growth.Therefore, identification of appropriate genes for genetic transformation and mutagenesis is a vital step, along with employing effective promoters [13][14][15][16] A plethora of genes, in the count of thousands, are reported to participate in the process of cell wall polymer synthesis, modification, and degradation [17].Several mutant plants showed improved growth yield and biomass degradation after genetic modifications when compared to the wild type (Figures 5 and 6).The genetic engineering approaches based on these genes have been summarized in the following section.

Wall Polymer Biosynthesis
Cellulose synthase complexes (CESA) are known to be protein complexes involved in the synthesis of cellulose in higher plants [18].These complexes, which are localized in the Golgi apparatus, synthesize cellulose in three steps after being transported to the plasma membrane.The three steps include the initiation of the β-1,4-glucan chain, followed by an elongation step, and completion of the process with the termination of the polymer chain [19].The CESA complex is formed by the CeSA gene family, which have been characterized in multiple plant species including Arabidopsis, rice, cotton, barley, and poplar [20].Some CeSA mutants have been explored by undertaking various genetic approaches to elaborate the biological functions of CeSA.However, maximum mutants resulted in reduced cellulose biosynthesis, accompanied by low crystallinity of the cell wall and abnormal plant growth.Overexpression of CesA genes led to a decreased biomass yield in transgenic plants of poplar and barley [21].Only the Osbc13 mutant in rice, which has a substituted amino acid in OsCesA9, showed normal plant growth despite reduced cellulose [22].
Another gene family responsible for the expression of glycosyltransferases (GTs) is known as the GT family, which governs the synthesis of hemicellulose along with cellulose synthase like (Csl) genes [17].Cellulose synthase like (Csl) genes encode glycan synthases located in the Golgi apparatus.The GT family is comprised of seven genes associated with the biosynthesis of hemicellulose.Several genetic mutants associated with hemicellulose synthesis have showcased a decreased biomass yield, along with improved digestibility and enzymatic saccharification [23].For instance, mutants of GT43 and GT47 degrade xylan backbones.

Wall Polymer Biosynthesis
Cellulose synthase complexes (CESA) are known to be protein complexes involved in the synthesis of cellulose in higher plants [18].These complexes, which are localized in the Golgi apparatus, synthesize cellulose in three steps after being transported to the plasma membrane.The three steps include the initiation of the β-1,4-glucan chain, followed by an elongation step, and completion of the process with the termination of the polymer chain [19].The CESA complex is formed by the CeSA gene family, which have been characterized in multiple plant species including Arabidopsis, rice, cotton, barley, and poplar [20].Some CeSA mutants have been explored by undertaking various genetic approaches to elaborate the biological functions of CeSA.However, maximum mutants resulted in reduced cellulose biosynthesis, accompanied by low crystallinity of the cell wall and abnormal plant growth.Overexpression of CesA genes led to a decreased biomass yield in transgenic plants of poplar and barley [21].Only the Osbc13 mutant in rice, which has a substituted amino acid in OsCesA9, showed normal plant growth despite reduced cellulose [22].
Another gene family responsible for the expression of glycosyltransferases (GTs) is known as the GT family, which governs the synthesis of hemicellulose along with cellulose synthase like (Csl) genes [17].Cellulose synthase like (Csl) genes encode glycan synthases located in the Golgi apparatus.The GT family is comprised of seven genes associated with the biosynthesis of hemicellulose.Several genetic mutants associated with hemicellulose synthesis have showcased a decreased biomass yield, along with improved digestibility and enzymatic saccharification [23].For instance, mutants of GT43 and GT47 degrade xylan backbones.
Lignin biosynthesis calls for the involvement of multiple enzymes to synthesize the three monomer units of lignin.These enzymes include phenylalanine ammonia lyase, 4-coumarate-CoA ligase, cinnamate 4-hydroxylase, shikimate hydroxycinnamoyl transferase, caffeoyl-CoA 3-O-methyltransferase (CCoAOMT), coumarate 3hydroxylase, cinnamoyl-CoA reductase (CCR), ferulate 5-hydroxylase, cinnamyl alcohol dehydrogenase, and caffeic acid/5-hydroxyferulic acid O-methyltransferase (COMT).Many genetic mutants associated with the biosynthesis of lignin have been explored in a variety of plant species.SbCOMTs, comprising four site mutations in COMT, show lower lignin synthesis, along with an enhanced biomass digestibility in sorghum [24].Similarly, Pv4CL1 gene silencing in switch grass plants also shows enhanced biomass digestibility by reducing the lignin content in the cell wall [25].However, the down regulation of the genes also affected the growth and stress tolerance of the plants, which demotes the adoption of the lignin reduction approach for modifications in energy crops.
Researchers have also investigated the effect of genes associated with pectin biosynthesis.Expression of the fungal polygalacturonase and pectin methylesterase inhibitor resulted in improved enzymatic saccharification in Arabidopsis [26].Similarly, suppressing the expression of pectin methylesterase (PME) resulted in a significant improvement in the biomass yield and enzymatic saccharification [2].Similar outcomes were obtained in the case of QUA2, GAUT12, and PME3, however, enhanced digestibility was accompanied by adverse effects on plant growth in transgenic plants of Arabidopsis and poplar [27].On the other hand, over expression of EXT and EXP genes not only enhanced biomass yield, but also improved its stress tolerance [28].However, the effect of these genes on enzymatic digestibility is yet to be explored.

Wall Polymer Degradation
The sequential process followed by plants for remodeling of their cell wall relies on the adequate expression of various enzymes.The process usually starts with the expansion and separation of cells in which pectins are targeted.Hydrolysis of cell wall components via programmed cell death is activated from surrounding cells by downstream signaling [29].This is followed by degradation of hemicellulose and cellulose.These common steps, required for cell wall degradation, are carried out by several enzymes and can be perceived as an integrated perspective.According to the modularity theory proposed by Grandis et al. [30], plants can be induced to alter the arrangement of their cell wall structure by overexpressing the key players from this step by step process.
Several enzymes are associated with cell wall degradation, such as endo-β-1,4-glucanases (EG), which cleaves the β-1,4-glycosidic links between glucose monomers.EG enzymes are part of the glycoside hydrolase 9 (GH9) family, which includes three subclasses according to enzyme specificity.Some of the mutants of this family, such as KORRIGON, PtCel9A1, OsGH9B etc., have been investigated for their effect on biomass yield and digestibility.KORRIGON mutants led to a reduced level of cellulose accompanied with abnormal plant growth, while PtCel9A1 mutants exhibited an increased biomass yield, with a reduction in cellulose content [6].Similarly, the AtGH9C2 gene silencing was also reported to reduce the crystallinity of cellulose, as well as enhancing the biomass yield [6].The OsGH9B mutant gene in rice also exhibited a significant correlation of gene expression with cellulose activity and the crystallinity index.
Apart from the GH9 enzyme, GH10 and GH11 enzymes were also reported to govern the degradation of hemicelluloses.In transgenic maize, overexpression of the GH11 gene resulted in improved biomass digestibility.On the other hand, RNAi silencing of the GH10 gene improved biomass yield in poplar [31].
Overexpression of PG and PL genes, associated with the degradation of pectin, enhanced the digestibility of biomass [32].However, this overexpression resulted in a negative impact on biomass yield.

Cell Wall Network Construction
Network construction in the cell wall involves the crosslinking and deposition of polymers.Several transcription factors, such as NAC, SND, MYB, VND, and SWN, are involved in regulating the synthesis of the wall polymer network [33].Overexpressing or silencing of genes encoding these factors can significantly affect the plant growth and biomass yield.As investigated by Petersen et al. [34], overexpression of the IRX gene, driven by the AtVND7 promoter, led to defective hemicellulose synthesis and resulted in an improved biomass yield and enzymatic digestibility.Overexpression of OsSWN1 improved biomass saccharification in rice [35].Moreover, the site-specific mutations in the MYB factor altered the cell wall composition, thereby, improving the biomass digestibility without compromising plant growth.The study concluded that the strategies of site-specific mutations and adequate promoters can be adopted for genetic modifications of the cell wall, regulating transcriptional factors.
Construction of the cell wall network can also be altered by modifying the presence of chemical entities responsible for the interlinkage of constituents.For example, the three-monomer unit of lignin are interlinked by ester, ether, and covalent bonds.Reduction in these linkages can affect the quantity of lignin content and the mechanical strength of plants.Ralph et al. [36] decreased the β aryl ether level by inducing site specific mutation in the C3H gene and observed significant changes in plant growth in alfalfa mutants.Wilkerson et al. [37] replaced the ether bonds with esters and ferulic conjugates by overexpression of the AsFMT gene and revealed enhanced saccharification with no negative impact on plant growth.
Furthermore, incorporation of soluble polysaccharides in the cell wall can significantly improve biomass digestibility without any adverse impact on plant cell growth.For example, the addition of hyaluronan synthase in tobacco resulted in improved hydrolysis of cellulose [38].Expression of the MLG synthase gene of rice in Arabidopsis incorporated β-1,3-1,4-glucans in the cell wall and resulted in a 42% increase of biomass saccharification [39].

Approaches for Genetic Manipulations
The genetic manipulation approach is based on target genes and the associated promoters in regulatory pathways (Table 2).Harris et al. [40] induced point mutation in the transmembrane domain region (C terminal) CESA1 A903V and CESA3 T942I in Arabidopsis thaliana.Sahoo et al. [41] obtained a transgenic tobacco plant by overexpressing the CESA3 gene from Arabidopsis thaliana L. They placed a CESA3 (ixr1-2) gene construct between the promoter M24 (proM24) and the rbcSE9 gene terminator.Handakumbura et al. [42] created loss-of-function mutants of BdCESA4 and BdCESA7 using microRNA constructs driven by a maize ubiquitin promoter (pUBI).Tan et al. [21] transformed barley plant by manipulating c DNA of HvCesA genes for the primary and secondary cell wall, driven by the CaMV 35S promoter.OsMYB103L, encoding the R2R3-MYB transcription factor in rice, was overexpressed, which further enhanced the expression of CESA genes to enhance cellulose content.On the other hand, knockdown of the gene OsMYB103L resulted in a lower expression of CESA genes, with cellulose content reduced by 13% [43] (Table 3).
Huang et al. [44] mutated gibberellin (GA) related genes for altering the expression of CESA genes and, consequently, the cellulose level.The down-regulation of CESA genes was observed in gibberellin deficient mutants.Chiniquy et al. [45] developed rice xax1 mutant plants, which were deficient in the aromatic compounds of ferulic acid and coumaric acid, thereby, reducing the presence of diferulic cross links.The mutant plants, therefore, exhibit enhanced saccharification.Chen et al. [7] developed a mutant rice plant in which dissociation transposon was inserted at the OsIRX10 locus to knock out the OsIRX10 gene that helps in the xylan backbone.The mutant rice revealed a 25% and 87% reduction in the thickness of the primary and secondary cell wall, respectively.A 10% reduction in xylose content was also observed due to a loss of the OsIRX10 gene function.Pawar et al. [46] revealed the regulation of the expression of the AnAXE1 gene by the 35S CAMV promoter and GT43B aspen promoter.The transgenic line revealed no significant change in wall composition.However, the xylan content, present in the cell wall, was observed to be more readily digestible by endogluconase.

Conclusions and Future Aspects
To maximize biomass yield and digestibility and obtain the desired valuable products there is a need to overcome the recalcitrance pertaining to the complex cell wall structure.In this regard, the manipulation of gene encoding of the proteins responsible for the regulation of the cell wall structure and composition seems to be an appropriate strategy.This strategy also holds significance since it not only brings about the desired changes in the current generation of transgenic plants, but also helps to stabilize those desired features in the generations which will evolve from these plants.The structural modifications resulted from altering the gene pool increase the digestibility of plants by altering the composition of cell wall constituents and the distribution of crystalline and amorphous regions.The challenge that persists in the adoption of genetic modification strategies is the adverse effects on the tolerance and growth of plants due to a reduction of constituents providing protection and mechanical strength to the plant.Therefore, intensive studies are required to identify the gene targets, along with the appropriate genetic modification approach to enhance the digestibility without compromising the natural protective mechanisms of plants, which are crucial for their survival and optimum growth.

Figure 5 .
Figure 5. Improved biomass yield after genetic modification.Figure 5. Improved biomass yield after genetic modification.

Figure 5 .
Figure 5. Improved biomass yield after genetic modification.Figure 5. Improved biomass yield after genetic modification.

Figure 6 .
Figure 6.Wild and mutant plants showing similar agronomy traits, but brittleness was observed in the mutant plant after genetic modification.

Figure 6 .
Figure 6.Wild and mutant plants showing similar agronomy traits, but brittleness was observed in the mutant plant after genetic modification.

Table 2 .
Wall polymers and genetic approaches for their modifications.

Table 3 .
Quantitative outcomes obtained after genetic modifications.