Zinc Oxide Nanoparticles Improve Salt Tolerance in Rice Seedlings by Improving Physiological and Biochemical Indices

: Understanding the salinity stress mechanisms is essential for crop improvement and sustainable agriculture. Salinity is prepotent abiotic stress compared with other abiotic stresses that decrease crop growth and development, reducing crop production and creating food security-related threats. Therefore, the input of metal oxide nanoparticles (NPs) such as zinc oxide nanoparticles (ZnO-NPs) can improve salt tolerance in crop plants, especially in the early stage of growth. Therefore, the aim of the current study was to evaluate the impact of ZnO-NPs on inducing salt tolerance in two rice ( Oryza sativa L.) genotypes of seedlings. An undocumented rice landrace (Kargi) and salinity tolerance basmati rice (CSR 30) seeds were grown in a hydroponic system for two weeks with and without 50 mg/L concentrations of ZnO-NPs in various doses of NaCl (0, 60, 80, and 100 mM). Both Kargi (15.95–42.49%) and CSR 30 (15.34–33.12%) genotypes showed a reduction in plant height and photosynthetic pigments (chlorophyll a and b, carotenoids, and total chlorophyll), Zn content, and K + uptake under stress condition, compared with control seedlings. On the other hand, stress upregulated proline, malondialdehyde (MDA), Na + content, and antioxidant enzyme activities—namely, those of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and glutathione reductase (GR)—in both O. sativa genotypes over the control. However, ZnO-NP-treated genotypes (Kargi and CSR 30) restored the photosynthetic pigment accumulation and K + level, reforming the stomata and trichome morphology, and also increased antioxidant enzymes APX, CAT, and GR activity, which alleviated the oxidative stress, while reducing the level of MDA, proline, and H 2 O 2 under stress condition. The present ﬁndings suggest that adding ZnO-NPs could mitigate the salinity stress in O. sativa by upregulating the antioxidative system and enhancing the cultivation of undocumented landrace (Kargi) and basmati (CSR 30) genotypes of O. sativa in salinity-affected areas.


Introduction
The human population is rapidly increasing, and it may reach approximately 10 billion by 2050. This increases in population may enhance food demand [1,2]. Continuously growing human population and changes in climate due to global warming may affect the prevailing farming practices, reducing crop production [2,3]. Currently, one-third of world agricultural lands suffer from salinization due to inappropriate agrochemical and

Effect of ZnO-NPs on the Early Stage of Rice under Salinity Stress Condition
Under salinity stress, a significant reduction in plant height was observed, by 15 (Table 1). However, the reduction rate in O. sativa seedling height in the Kargi genotype was slightly higher than that in CSR 30 in terms of both ZnO-NPs and stress conditions. At the same time, improvement was observed in 100 mM NaCl with ZnO-NPs, compared with the corresponding treatment without ZnO-NPs. However, the other two salinity treatments, i.e., 60 and 80 mM NaCl, did not show any significant difference, compared with their complementary ZnO-NPs treatment. Values are the means of three replicates' standard deviation (±SD). Different small letters (a-d) within the row indicate a statistically significant value (LSD) at p ≤ 0.01 among the treatments.

Na + and K + Concentrations Analysis
An increased Na + and decreased K + amount were observed, compared with control, under salinity stress.
Applying ZnO-NPs alleviated the salinity stress by decreasing the Na + and increasing the K + uptake. Under salinity stress conditions, the Na + concentration significantly increased between 337.74% and 754.72% in Kargi, and 247.27% and 608.73% in CSR 30 leaves, compared with control ( Figure 1A). After the application of ZnO-NPs, a reduction in Na + concentration was found in the leaves of Kargi (166.04-418.87%) and CSR 30 .82%) in comparison to salt-stressed and nonstressed control O. sativa seedling ( Figure 1A). Under salinity stress, the level of K + decreased in leaves of Kargi (70.96-79.04%) and CSR 30 (63.82-76.73%), respectively, in comparison to control ( Figure 1B). However, after the input of ZnO-NPs, the K + concentration was 8. 73-19.42% in Kargi and 6.18-17.64% in CSR 30 leaves, compared with salt-stressed and nonstressed plants, respectively ( Figure  1B).
The observation of the Na + /K + ratio indicated a higher Na + /K + ratio in Kargi and CSR 30 under the saline conditions, and minimum changes were observed in ZnO-NP-treated O. sativa seedling ( Figure 1C). However, in NaCl and ZnO-NP treatments, a slight increase in Na + /K + ratio was observed in Kargi, compared with CSR 30 genotypes of O. sativa (Figure 1C). Figure 1. Impact of ZnO-NPs on (A) Na + (B) K + and (C) Na + /K + ratio concentration in leaves of Kargi and CSR 30 Oryza sativa genotype in control and saline conditions. Different small alphabets (a-g) indicate a statistically significant difference between treatments concerning control. Values are means of three replicates. Error bars indicate the least considerable value (LSD) at p ≤ 0.01 among the treatments. Under salinity stress, the level of K + decreased in leaves of Kargi (70.96-79.04%) and CSR 30 (63.82-76.73%), respectively, in comparison to control ( Figure 1B). However, after the input of ZnO-NPs, the K + concentration was 8.73-19.42% in Kargi and 6.18-17.64% in CSR 30 leaves, compared with salt-stressed and nonstressed plants, respectively ( Figure 1B).

Proline Accumulation
The observation of the Na + /K + ratio indicated a higher Na + /K + ratio in Kargi and CSR 30 under the saline conditions, and minimum changes were observed in ZnO-NP-treated O. sativa seedling ( Figure 1C). However, in NaCl and ZnO-NP treatments, a slight increase in Na + /K + ratio was observed in Kargi, compared with CSR 30 genotypes of O. sativa ( Figure 1C).

Proline Accumulation
The increased proline accumulation in the leaves of Kargi and CSR 30 was noted in salinity stress conditions. It rose from 240.91% to 363.64% in Kargi and from 225% to 375.02% in CSR 30 under stress conditions, compared with control seedlings (Figure 2). Under saline conditions with the combination of ZnO-NPs, the level of proline in leaves decreased in Kargi from 36.36% to 104.55% and in CSR 30 from 25% to 100%, compared with the control treatment ( Figure 2). Less proline accumulation in ZnO-NP-treated Kargi and CSR 30 leaves indicates physiological adaptation under saline conditions. A high accumulation of proline in Kargi, compared with that in CSR 30, was found with both saline and ZnO-NP treatments. The increased proline accumulation in the leaves of Kargi and CSR 30 was noted in salinity stress conditions. It rose from 240.91% to 363.64% in Kargi and from 225% to 375.02% in CSR 30 under stress conditions, compared with control seedlings (Figure 2). Under saline conditions with the combination of ZnO-NPs, the level of proline in leaves decreased in Kargi from 36.36% to 104.55% and in CSR 30 from 25% to 100%, compared with the control treatment ( Figure 2). Less proline accumulation in ZnO-NP-treated Kargi and CSR 30 leaves indicates physiological adaptation under saline conditions. A high accumulation of proline in Kargi, compared with that in CSR 30, was found with both saline and ZnO-NP treatments. Figure 2. Impact of ZnO-NPs on proline accumulation in Kargi and CSR 30 leaves in control and under saline conditions. Values are means of three replicates; a statistically significant difference between treatments compared with control is indicated by different small alphabets (a-f). Error bars indicate the least considerable value (LSD) at p ≤ 0.01 among the treatments.

Hydrogen Peroxide (H2O2) Content
High accumulation of H2O2 was observed under saline conditions, which was manifested as 50-150% in Kargi and 50-133.33% in CSR 30, respectively, compared with control treatments (Figure 3). However, a significant decline in H2O2 accumulation was found in ZnO-NP treatments between 16.67% and 50% in Kargi and between 20% and 50% in CSR 30 leaves, compared with nonstressed control O. sativa seedlings ( Figure 3).

Figure 2.
Impact of ZnO-NPs on proline accumulation in Kargi and CSR 30 leaves in control and under saline conditions. Values are means of three replicates; a statistically significant difference between treatments compared with control is indicated by different small alphabets (a-f). Error bars indicate the least considerable value (LSD) at p ≤ 0.01 among the treatments.

Hydrogen Peroxide (H 2 O 2 ) Content
High accumulation of H 2 O 2 was observed under saline conditions, which was manifested as 50-150% in Kargi and 50-133.33% in CSR 30, respectively, compared with control treatments (Figure 3). However, a significant decline in H 2 O 2 accumulation was found in ZnO-NP treatments between 16.67% and 50% in Kargi and between 20% and 50% in CSR 30 leaves, compared with nonstressed control O. sativa seedlings ( Figure 3).

MDA (Malondialdehyde) Content
The MDA level was increased in leaves of Kargi (17.20-47.31%) and CSR 30 (15.23-45.23%) under salinity stress ( Figure 4). However, the addition of ZnO-NPs varied the MDA content significantly between 9.67% and 19.35% in Kargi and between 8.85% and 12.50% in CSR 30 under salinity stress, compared with control plants (Figure 4). Specifically, the MDA concentration in Kargi was slightly higher than that in CSR 30 with both saline and ZnO-NP treatments. Conversely, in ZnO-NP treatments, the concentration of MDA was decreased in both Oryza sativa genotypes.

MDA (Malondialdehyde) Content
The MDA level was increased in leaves of Kargi (17.20-47.31%) and CSR 30 (15.23-45.23%) under salinity stress ( Figure 4). However, the addition of ZnO-NPs varied the MDA content significantly between 9.67% and 19.35% in Kargi and between 8.85% and 12.50% in CSR 30 under salinity stress, compared with control plants (Figure 4). Specifically, the MDA concentration in Kargi was slightly higher than that in CSR 30 with both saline and ZnO-NP treatments. Conversely, in ZnO-NP treatments, the concentration of MDA was decreased in both Oryza sativa genotypes.

MDA (Malondialdehyde) Content
The MDA level was increased in leaves of Kargi (17.20-47.31%) and CSR 30 (15.23-45.23%) under salinity stress ( Figure 4). However, the addition of ZnO-NPs varied the MDA content significantly between 9.67% and 19.35% in Kargi and between 8.85% and 12.50% in CSR 30 under salinity stress, compared with control plants (Figure 4). Specifically, the MDA concentration in Kargi was slightly higher than that in CSR 30 with both saline and ZnO-NP treatments. Conversely, in ZnO-NP treatments, the concentration of MDA was decreased in both Oryza sativa genotypes.  Error bars indicate the least considerable value (LSD) at p ≤ 0.01 among the treatments.

Zn Content
In saline treatments, accumulation of Zn content was significantly decreased, by 18.20-56.52% in Kargi and 12.82-48.72% in CSR 30 leaves, compared with nonstressed plants ( Figure 5). After the addition of external Zn supply in the form of ZnO-NPs, increased Zn content was observed in Kargi (212.82-133.08%) and CSR 30 (212.50-139.13%) leaves ( Figure 5). Zn accumulation in saline and ZnO-NP treatments was slightly lower in Kargi than in CSR 30. In saline treatments, accumulation of Zn content was significantly decreased, by 18.20-56.52% in Kargi and 12.82-48.72% in CSR 30 leaves, compared with nonstressed plants ( Figure 5). After the addition of external Zn supply in the form of ZnO-NPs, increased Zn content was observed in Kargi (212.82-133.08%) and CSR 30 (212.50-139.13%) leaves ( Figure 5). Zn accumulation in saline and ZnO-NP treatments was slightly lower in Kargi than in CSR 30.

Antioxidant Enzyme Activity
A clear change was found in the antioxidant enzyme activity in Kargi and CSR 30 with saline and ZnO-NP treatments. NaCl-treated Kargi and CSR 30 seedlings showed increased antioxidant enzyme activity, compared with those using nonstressed treatments. Furthermore, using ZnO-NPs enhanced antioxidant enzyme activities, compared with both saline and nonsaline treatments.

Antioxidant Enzyme Activity
A clear change was found in the antioxidant enzyme activity in Kargi and CSR 30 with saline and ZnO-NP treatments. NaCl-treated Kargi and CSR 30 seedlings showed increased antioxidant enzyme activity, compared with those using nonstressed treatments. Furthermore, using ZnO-NPs enhanced antioxidant enzyme activities, compared with both saline and nonsaline treatments. The SOD functions as the first line of defense under stress conditions, which significantly increased under saline conditions, by 23.69-31% in leaves of Kargi and 35.51-52.06% in CSR 30, compared with that observed with nonstressed treatment ( Figure 7A). Treatment with ZnO-NPs showed a further increment in SOD. The activity of SOD in leaves was noted by 37.58-60.99% in Kargi and 47.42-70.23% in CSR 30, respectively, compared with that found with stressed and nonstressed treatments ( Figure 7A). Compared with all treatments, the activity of SOD was slightly less in Kargi than in CSR 30. Under saline conditions, a marked increase in CAT activity was found in the leaves of Kargi (12.65-68.67%) and CSR 30 (33.93-91.07%) in stress conditions, compared with that found with nonstressed treatments ( Figure 7B). All treatments showed a noticeable increase in CAT activity under stressed and nonstressed environments. However, a prominent increase was revealed in ZnO-NP treatments of Kargi (50.40-120.48%) and CSR 30 (57.74-129.17%), compared with their saline and untreated control seedlings ( Figure 7B). CAT activity was slightly lesser in all the treatments of Kargi than that of CSR 30. Under saline conditions, a marked increase in CAT activity was found in the leaves of Kargi (12.65-68.67%) and CSR 30 (33.93-91.07%) in stress conditions, compared with that found with nonstressed treatments ( Figure 7B). All treatments showed a noticeable increase in CAT activity under stressed and nonstressed environments. However, a prominent increase was revealed in ZnO-NP treatments of Kargi (50.40-120.48%) and CSR 30 (57.74-129.17%), compared with their saline and untreated control seedlings ( Figure 7B). CAT activity was slightly lesser in all the treatments of Kargi than that of CSR 30. In all of the saline treatments, a significantly high level of APX activity was shown in Kargi (13.21-32.45%) and CSR 30 (15.79-36.84%) leaves, compared with that found with nonsaline treatment ( Figure 7C). All saline treatments showed a remarkable increase in APX activity in both genotypes. However, in ZnO-NP treatments, high activity of APX was found in both Kargi (20.36-44.56%) and CSR 30 (26.32-47.37%) O. sativa genotypes, compared with those in saline and nonstressed treatments ( Figure 7C). In Kargi, the level of APX was slightly decreased in all of the treatments, compared with that of CSR 30.
2.8.4. GR (EC 1.8.1.7) Salinity-exposed leaves showed a high activity of GR enzymes by 3.55-10.44% in Kargi and 5.85-11.88% in CSR 30, compared with those under nonstressed conditions ( Figure 7D). The addition of ZnO-NPs upregulated the activity of GR in Kargi (5.66-11.85%) and CSR 30 (6.96-12.38%) leaves ( Figure 7D). Although all treatments showed a maximum activity of GR enzyme, higher activity was demonstrated in ZnO-NP-treated treatments, compared with that observed under saline conditions. In addition, the GR activity was slightly higher in CSR 30 than in Kargi for all treatments. In all of the saline treatments, a significantly high level of APX activity was shown in Kargi (13.21-32.45%) and CSR 30 (15.79-36.84%) leaves, compared with that found with nonsaline treatment ( Figure 7C). All saline treatments showed a remarkable increase in APX activity in both genotypes. However, in ZnO-NP treatments, high activity of APX was found in both Kargi (20.36-44.56%) and CSR 30 (26.32-47.37%) O. sativa genotypes, compared with those in saline and nonstressed treatments ( Figure 7C). In Kargi, the level of APX was slightly decreased in all of the treatments, compared with that of CSR 30.
2.8.4. GR (EC 1.8.1.7) Salinity-exposed leaves showed a high activity of GR enzymes by 3.55-10.44% in Kargi and 5.85-11.88% in CSR 30, compared with those under nonstressed conditions (Figure 7D). The addition of ZnO-NPs upregulated the activity of GR in Kargi (5.66-11.85%) and CSR 30 (6.96-12.38%) leaves ( Figure 7D). Although all treatments showed a maximum activity of GR enzyme, higher activity was demonstrated in ZnO-NP-treated treatments, compared with that observed under saline conditions. In addition, the GR activity was slightly higher in CSR 30 than in Kargi for all treatments.

Impact of ZnO-NPs on Stomata and Trichome Morphology
Scanning electron microscopy (SEM) observations of Kargi and CSR 30 leaves showed deformation in the stomata and trichome morphology under NaCl-treated conditions ( Figure 8). The images obtained using SEM indicated that the reformation phenomena of stomata (Figure 8 A

Discussion
In the past few years, the application of nanotechnology has rapidly increased in agriculture, including in abiotic stress tolerance approaches [35]. Applying ZnO-NPs significantly increased resistance against salinity stress in Kargi and CSR 30 genotypes of O. sativa by improving the biosynthesis o photosynthetic pigments, increasing the uptake of K + ions, and reducing the level of Na + ions, ROS, H2O2, and MDA by enhancing the activity of antioxidant enzymes [35,60]. The finding of Hafeez et al. [61] also suggested that Zn facilitates the regulation and stabilization of cell membranes by binding with phospholipids and sulfhydryl groups, mainly when plants experience stress conditions. Research findings in various crops revealed a positive impact of ZnO-NPs by improving physiological and biological actives under abiotic stress, e.g., heavy metal, salinity, chilling, and drought stress [35,[62][63][64][65][66][67][68]. Furthermore, ZnO-NPs can contribute to forming organic compounds such as IAA and GA3, directly participating in plants' growth [69]. It also maintains the membrane structural stability, protein and enzyme activation, and cell prolongation and provides the tolerance mechanism against different types of ecological stress. These positive effects of ZnO-NPs may explain why zinc nanotreatment can improve O. sativa growth under salinity stress [69][70][71].
On the other hand, many studies showed that applying ZnO-NPs also create phytotoxicity on crops that inhibit their growth and development [47,48,72]. For example, Lopez-Moreno et al. [73] demonstrated that the application of 2000 mg/LZnO-NPs on Glycine max led to genotoxicity that affected the integrity of the DNA. The findings of Lee et

Discussion
In the past few years, the application of nanotechnology has rapidly increased in agriculture, including in abiotic stress tolerance approaches [35]. Applying ZnO-NPs significantly increased resistance against salinity stress in Kargi and CSR 30 genotypes of O. sativa by improving the biosynthesis o photosynthetic pigments, increasing the uptake of K + ions, and reducing the level of Na + ions, ROS, H 2 O 2 , and MDA by enhancing the activity of antioxidant enzymes [35,60]. The finding of Hafeez et al. [61] also suggested that Zn facilitates the regulation and stabilization of cell membranes by binding with phospholipids and sulfhydryl groups, mainly when plants experience stress conditions. Research findings in various crops revealed a positive impact of ZnO-NPs by improving physiological and biological actives under abiotic stress, e.g., heavy metal, salinity, chilling, and drought stress [35,[62][63][64][65][66][67][68]. Furthermore, ZnO-NPs can contribute to forming organic compounds such as IAA and GA3, directly participating in plants' growth [69]. It also maintains the membrane structural stability, protein and enzyme activation, and cell prolongation and provides the tolerance mechanism against different types of ecological stress. These positive effects of ZnO-NPs may explain why zinc nanotreatment can improve O. sativa growth under salinity stress [69][70][71].
On the other hand, many studies showed that applying ZnO-NPs also create phytotoxicity on crops that inhibit their growth and development [47,48,72]. For example, Lopez-Moreno et al. [73] demonstrated that the application of 2000 mg/L ZnO-NPs on Glycine max led to genotoxicity that affected the integrity of the DNA. Further studies have suggested that low and control applications of ZnO-NPs positively affect plants under salinity stress. Hezaveh et al. [74] recommended a value of 20 mg/L to alleviate the effect of salinity stress on the Brassica napus cultivar at the flowering stage. Recently, Mogazy et al. [75] demonstrated that 50 mg/L ZnO-NPs with NaCl could enhance the growth and antioxidant activity in Vicia faba under salinity stress. Similarly, in our study, a low concentration of ZnO-NPs (50 mg/L) was used and did not exert a negative impact on O. sativa genotypes.
In abiotic stress biology, several realistic models or in vitro experimental results showed that applying ZnO-NPs could increase the germination and yield of cereal crops, including O. sativa. Zhang et al. [63] showed enhancement in the O. sativa yield, ranging from 2.5% to 11.8%, by applying ZnO-NPs. The low concentration of ZnO-NPs in the hydroponic system showed better growth of Hordeum vulgare [76]. In another study, Upadhyaya et al. [65] reported that exposure to O. sativa seeds with ZnO-NP (5-50 mg/L) showed excellent potential for better germination of O. sativa. The foliar application of ZnO-NPs (5.0 g/L) significantly improved the growth and yield of O. sativa [77]. These findings concluded that applying ZnO-NPs positively impacted rice under normal conditions at low dose additions. Thus, our findings on the impact of ZnO-NPs under salinity stress could be an addition to managing damage during the early stage of O. sativa growth.
The present study revealed that the addition of ZnO-NPs led to the regulation of the salinity tolerance in Kargi and CSR 30 under controlled conditions [78,79]. The concentration of Zn content decreased in saline treatments in both cultivars. The supply of external Zn in the form of ZnO-NPs improved the level of Zn in considered O. sativa genotypes. Our results correlate with the findings of another study indicating that the input of ZnO-NPs increased Zn content by 13.5-39.4% in O. sativa, compared with conventional fertilization applications, and is safe for human consumption. [49]. Our results also showed that the increased Zn content stimulated the growth of Kargi and CSR 30 seedlings under salinity stress. At the same time, other studies showed that, without applying ZnO-NPs, salinity reduced O. sativa growth by reducing the uptake of Zn [65,[80][81][82]. Our results agree with earlier findings that reported external application of ZnO-NPs improved O. sativa growth [65,82,83].
Kargi and CSR 30 growth were affected because salinity disrupts the uptake mechanism of potassium nutrients and stores excessive Na + ions in both O. sativa genotypes leaves, decreasing the level of K + and increasing the Na + /K + ratio. In living cells, potassium helps maintain the turgidity and regulation of essential enzyme activity of the cell. At the same time, the shortage of K + ions and the high Na + /K + ratio inhibits the growth and development of plants [84][85][86][87]. Therefore, applying ZnO-NPs promoted better adaptation toward salinity stress in Kargi and CSR 30 by lowering the Na+ concentration and Na + /K + ratio in their leaves and improving the K + ion concentration. Improving the Na +/ K + balance is a key trait of salinity tolerance for plants to better adapt under salinity stress conditions [85]. Similar results on Zea mays and Gossypium spp. showed that the application of ZnO-NPs lowered the Na + and Na + /K + ratio, which enhanced K + concentration, resulting in improvement of growth under salinity stress [88,89].
Proline is the most prominent organic solute that acts as an osmolyte and maintains the cytosolic pH and osmotic condition of cells during salinity stress [90]. The current study observed increased proline content in NaCl-treated Kargi and CSR 30. It was also suggested that the amount of proline increased in Trigonella foenum-graecum and Gossypium spp. under salinity stress [89]. In our study, adding ZnO-NPs (50 mg/L) decreased proline accumulation. In Abelmoschus esculentus and Citrus spp., reduced proline was noted under saline conditions by applying ZnO-NPs [53]. These results correspond with our findings on O. sativa seedlings.
The higher accumulation of Na + triggers ROS production, leading to membrane lipid peroxidation. The final lipid peroxidation product is MDA during oxidative stress, damaging the plant's membrane [91][92][93][94]. In this study, the higher MDA accumulation was found in Kargi and CSR 30 under saline conditions, and applying ZnO-NPs lowered its accumulation under salinity conditions. The decrease in MDA content could be an adaptation process of O. sativa seedlings under salinity stress. It was explained earlier that applying ZnO-NPs can play a defensive role in coping with salinity stress by reducing the concentration of MDA, which helps in the improvement and permeability of the membrane and oxidative stress in plant seedlings [95]. Few findings also suggested that salinity stress disrupted the lipid and protein composition of the membrane that affected the architecture of the leaves' cell membrane [93,94,[96][97][98].
Salinity directly affects the chloroplast structure and denatures their membrane and the rate of photosynthesis, which results in low grain formation and poor yield [56,99]. Furthermore, salinity stress alters the function of stomata by reducing their structure, process, and density [100]. This alteration reduces the rate of CO 2 uptake, decreases the photosynthesis rate, and the number of chloroplasts that damage the grana and thylakoid membrane directly hit the photosynthetic pigment-protein machinery of photosynthesis [101][102][103][104].
In current findings, the photosynthetic pigments-namely, Chl a, Chl b, total chlorophyll, and carotenoids-showed a remarkable reduction in Kargi and CSR 30 under salinity stress. At the same time, the application ZnO-NPs under saline conditions enhanced chlorophyll a and b, carotenoid, and total chlorophyll. Our finding was supported by those of Alabdallah et al. [53] and Venkatachalam et al. [105], which revealed that ZnO-NPs improved the photosynthetic pigments in A. esculentus and Gossypium spp. under saline conditions. This result of enhancement in photosynthetic pigment content in ZnO-NPtreated Kargi and CSR 30 may be due to the upregulation of ROS-scavenging enzymes that rapidly degrade the ROS radicals present in thylakoid membranes of chloroplasts [94,106] and likely another reason supporting current findings, i.e., reduction in the Na + /K + ratio in both ZnO-NP-treated O. sativa genotypes helps to maintain ionic homeostasis of cells and upregulation of chlorophyllase activity [107,108].
An excessive accumulation of NaCl creates oxidative stress that produces ROS, e.g., O 2 •− , 1 O 2 , H 2 O 2 , and OH • [109][110][111]. ROS is destructive and can damage the cell and its organization. To overcome this problem, antioxidant enzymes (SOD, CAT, APX, and GR) come into play and help detoxify ROS radicals [22]. In this study, SOD, CAT, APX, and GR activity were higher in Kargi and CSR 30 under saline conditions. Furthermore, the higher activity of these antioxidant enzymes indicated the greater adaptation of Kargi and CSR 30 under salinity stress.
Similarly, rice under salinity stress was found to have increased antioxidant enzyme activity [96]. Further, in our study, the applications of ZnO-NPs upregulated the antioxidant enzyme activity more in ZnO-NP-treated plants than those grown in normal saline conditions without NPs. Similarities to our results were also found in results indicating that the application of ZnO-NPs upregulated the activity of antioxidant enzymes in A. esculentus and Z. mays under salinity stress [53]. Salinity affected the morphology of the stomatal aperture and trichome of plant leaves under salinity stress [112][113][114][115][116]. Reduction in the morphology of stomata and trichome affects the uptake of CO 2 from the atmosphere, reducing the photosynthesis rate [76]. An improvement in the morphology of trichomes and structure and density of stomata were noted in Kargi and CSR 30 under salinity with the addition of ZnO-NPs. The application ZnO-NPs improved the morphology of the stomatal aperture and trichome in spring barley under hydroponic conditions. However, the authors also showed that these subcellular organelles were damaged with high concentrations of NPs [76].

Materials and Methods
Commercial-grade zinc oxide (ZnO) nanopowder (particle size < 50 nm; batch no, 6063960) was purchased from Sisco Research Laboratories (SRL.) Pvt. Ltd., Maharashtra, India, which was used following a previously described protocol [77]. The seeds of Kargi landrace (IC-0642936) were collected from a farmer of Pauha village Machhlishahr, Jaunpur, Uttar Pradesh, India, and CSR 30 salt-tolerant basmati cultivar was obtained from Basmati Export Development Foundation, Meerut, India. The genotype CSR 30 is a salt-tolerant type of basmati rice, immensely popular among farmers and released by Central Soil Salinity Research Institute (CSSRI), Haryana, India. The variety has the potential to grow under saline and sodic environments [117]. In contrast, the other genotype in this study, Kargi, is an undocumented, rare, and on-the-verge-of-extinction rice landrace mostly cultivated in saline lands in the Eastern part of Uttar Pradesh, India. Farmers who traditionally cultivated Kargi O. sativa claimed it can grow in saline areas. For this reason, documentation of this landrace becomes more important for crop improvement and rice production in saline areas.
Kargi and CSR 30 seeds were sterilized with 5% sodium hypochlorite solution for 30 min and washed with distilled water. After washing, 25 seeds of both rice genotypes were transferred to a round piece of wet filter paper in clean Petri dishes and kept in an incubator at 30 • C for germination for five days. All treatments were performed in triplicate.

Growth Parameters
The treated O. sativa seedlings (in triplicate) were collected, and plant height (cm) of the different treatments was measured using a meter scale. Images regarding the growth of (A) Kargi and (B) CSR 30 rice genotypes after two weeks of treatments are presented in Supplementary File S1.

Estimation of Na + and K + Concentrations
The Na + and K + concentrations in the leaves of Kargi-and CSR-30-treated O. sativa genotypes were determined using a flame photometer (Elico-CL36, Hyderabad, India), as described by Abdelhamid et al. [79]. The dried rice leaves were placed in 1 N HCl for 12 h, and the concentrations of Na + and K + were estimated from the Na + and K + standard curves.

Estimation of Proline Content
The proline content of Kargi and CSR 30 fresh leaves was estimated per the protocol of Bates et al. 1973 [118]. Fresh Kargi and CSR 30 leaves (500 mg) were extracted in a sulfosalicylic acid solution, adding an equal amount of ninhydrin and glacial acetic acid solution. The sample was heated at 100 • C and added 5 mL of toluene, and the absorption was recorded at 520 nm using toluene as a blank. Proline is expressed as µg/g FW.

Estimation of Hydrogen Peroxide (H 2 O 2 )
The levels of H 2 O 2 in Kargi and CSR 30 leaves were determined based on the study of Velikova et al. [119]. Leaves of Kargi and CSR 30 (0.5 g) genotypes of O. sativa seedlings were homogenized in an ice bath with 5 mL TCA (0.1% w/v). Centrifuged at 12,000× g for 15 min. After that, in 0.5 mL of the supernatant, 1 mL 1 M KI and 0.5 mL to 10 mM potassium phosphate buffer with pH 7.0 were added. The absorbance of the supernatant was measured at 390 nm.

Estimation of Zn Content
To analyze Zn content in treated O. sativa seedling leaves, they were ground into a fine powder and weighed; then, 0.5 g ground samples were transferred to 10 mL of a mixture of nitric acid and perchloric acid at a ratio of 2:1. This pre-digestion mixture was left overnight. After 24 h, these flasks were placed on a hot plate, and the sample was heated at 150-235 • C until the time at which orange fumes were converted into white fumes. The color change indicated complete digestion of the leave samples. Further, it was filtered by adding 2-3 mL of deionized water into 50 mL glass volumetric flasks and making up the volume by adding more deionized water into 100 mL flasks. The filtered extract was analyzed on an atomic absorption spectrometer (GBC-SavantAA-01-1006-03, Mumbai, India) [82].

Estimation of Photosynthetic Pigments
For observation of photosynthetic pigment contents (chlorophyll (Chl) a, Chl b, and carotenoids) in Kargi and CSR 30 O. sativa leaves, 100 mg of fresh leaves of both genotypes were weighed and ground into 10 mL of 80% (v/v) acetone. After grinding filter extract into 50 mL of the volumetric flask with the help of Whatman filter paper and funnel, the final volume was made with the help of 80% acetone [122,123]. Next, the absorbance of the extract was read at 645, 663, and 470 nm using acetone (80%) blank by using the following method: where OD indicates optimal density.

Estimation of Antioxidant Enzymes' Activity
In Kargi and CSR 30 leaves, the SOD, CAT, and APX activities were measured according to the modified method of Takagi and Yamada [124]. One milliliter of crude enzyme extracts in a dialysis membrane was dialyzed in 500 mL of 10 mM K-P buffer (pH 7.8) for 12 h and renewed the K-P buffer every three hours. After that, this dialyzed extracted enzyme was used to measure the SOD activity at 560 nm absorbance.
The activity of CAT was measured using the previously described method by Tanaka et al. [125]. First, the CAT activity levels in Kargi and CSR 30 leaves were measured in 1 mL of assay mixture containing 100 mM K-P buffer (pH 7.8), 20 mM H 2 O 2, and 2% (volume/volume (v/v)) crude enzyme extracts. After that, the activity of the CAT enzyme was measured by determining the amount of scavenged H 2 O 2 per minute, and this was measured at specific absorbance of 230 nm.
The activity of APX was measured in 1 mL of assay mixture that had 100 mM K-P buffer (pH 7.8), 0.5 mM L-ascorbic acid, and 2% (v/v) crude enzyme extracts. Afterward, the oxidized L-ascorbic acid per minute was measured at a specific 290 nm absorbance.
The activity of GR was measured in 1 mL of assay mixture containing 50 mM K-P buffer (pH 7.8), 0.1 mM ethylenediaminetetraacetic acid (EDTA), 0.02 mM reduced nicotinamide adenine dinucleotide phosphate (NADPH), 0.02 mM oxidized form of glutathione, and 5% (v/v) crude enzyme extract. After that, NADPH's oxidized form was measured at a specific 340 nm absorbance to determine GR's activity.

Stomata and Trichome Morphology Observed via Scanning Electron Microscopy
The response and morphology of rice genotype CSR 30 and Kargi stomata and trichome under the different treatments of NaCl and ZnO-NPs were investigated using SEM (Zeiss EVO LS10, Oberkochen, Germany) in high vacuum water-free and covered with a thin silver metal layer at 20 kv.

Statistical Analyses
The statistical analyses were performed by one-way ANOVA using SPSS software 16.0 Version, and every experiment's technical specifications were triplicated. Significant differences in means were compared using Duncan's test at a significance level of p ≤ 0.01.

Conclusions
This study highlighted that the salinity tolerance in Oryza sativa depends on Na + exclusion from leaves, balancing the Na + /K + ratio, lowering the concentration of MDA, closing the stomata to avoid excessive water loss, and accelerating the activity of the antioxidant enzymes to cope-up stress. The application of ZnO-NPs upregulates salinity adaption processes. A comparative study between Kargi and CSR 30 under saline conditions demonstrated that Kargi rice genotypes were slightly less adapted under saline conditions because CSR 30 genotypes of O. sativa had better stomatal and trichome morphology and slightly higher antioxidant enzyme activities, photosynthesis pigments content, and Zn accumulation in all the treatments. However, with ZnO-NPs application, Kargi performed better under saline conditions. Cultivation of Kargi in saline areas with applying ZnO-NPs may result in better performance and production. Moreover, real-world field experiments on the performance of ZnO-NPs in the Kargi landrace, especially on degraded soils, should be performed in detail. Furthermore, in O. sativa, few genes are correlated with antioxidant enzyme activity, which works under environmental stress conditions. Therefore, expression analysis of genes that encode antioxidant enzymes related to the salinity tolerance of Kargi-namely, Na + and K + transporters-should be investigated.

Patents
National Identity or IC Number (Indigenous Collection), IC-0642936, of landrace rice genotype Kargi was allotted by the National Bureau of Plant Genetic Resources-ICAR New Delhi, India.

Data Availability Statement:
The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.