Yield and Chemical Composition of Raw Material from Meadow Arnica ( Arnica chamissonis Less.) Depending on Soil Conditions and Nitrogen Fertilization

: Considering the global tendency towards using valuable secondary metabolites of plant origin, there is a need to optimize the cultivation of herbal plants, e.g., the meadow arnica ( A. chamissonis Less.). The lactones, ﬂavonoids, and essential oils, and the yields of metabolites presented in this study indicate favorable climatic conditions for the cultivation of A. chamissonis in Central and Eastern Poland. This proves that a wide range of pharmacologically active substances can be produced in this region of Europe.


Introduction
The meadow arnica (Arnica chamissonis Less.) is a perennial plant from the family Asteraceae with a native occurrence range covering the western regions of North America and British Columbia [1,2]. Its natural habitats are meadows, banks of streams, and alpine and subalpine forests with scattered tree stands [3,4]. With its large adaptive potential [5], the species can be successfully cultivated in other regions of the world with the temperate climate. To date, attempts have been made to cultivate meadow arnica in Europe, e.g., Poland, Serbia, and Lithuania [6][7][8]. Flower heads that can be used for pharmaceutical and the biosynthesis of lactones: Sources with a predominance of nitrates over the ammonium form are considered more favorable.
Generally, nitrogen application significantly increases crop growth parameters such as plant height, leaf area, and biomass. Research results reported in the literature do not clearly show only a positive response of herbal plants to nitrogen fertilization. The trade-off principle is observed, as in the case of the noticeably higher mass but lower content of flavonoids in Calendula officinalis L. [57,58]. Therefore, an increase in the nitrogen dose may increase biomass production and can reduce the concentration and yield of secondary metabolites. Hence, it is important to investigate the quantitative parameters of raw materials and the concentration and yield of secondary metabolites. These aspects are addressed in the present study of the A. chamissonis response to increasing nitrogen doses.
Currently, special attention is given in agriculture to the wide-scale adoptions of lowinput, soil-friendly, and resource-conserving agronomic practices, which are imperative for reducing carbon emissions and nitrogen losses from agricultural soil and reducing eutrophication. Sustainable agriculture strives for maintaining or even increasing productivity, quality, and economic viability, while leaving a minimal footprint on the environment [72,73]. Currently, in the field production of medicinal plants, it is necessary to reduce the doses of mineral fertilizers and pesticides [74]. To improve the use of N fertilizers and reduce environmental pollution in agriculture, it is essential to optimize the N fertilization rate, thereby meeting the actual requirements of plants and simultaneously reducing N fertilization substantially. Optimal doses of nitrogen application can be considered as a relevant method for modification of not only morphological traits but also the chemical composition in plants. They can also contribute to the increased production of secondary metabolites in controlled field conditions [10].
With the content of numerous pharmacologically active substances in its flower heads, A. chamissonis is a pharmaceutical substitute for the endangered A. montana [3,9,29,36,75,76]. Moreover, the meadow arnica can be cultivated more easily than the mountain arnica. Therefore, in the last decades, various agricultural factors have been studied in field conditions. Row spacing and the maturity of flower heads were found to have a significant impact on higher yields of flower heads and such secondary metabolites as sesquiterpene lactones, flavonoids, and essential oils [9,37]. Foliar boron fertilization is another factor affecting the essential oil content in Arnica chamissonis flower heads [76]; however, the variation in the content of basic metabolites in meadow arnica flower heads under nitrogen fertilization in different soil conditions has not been investigated. Therefore, the objective of this study was (i) to evaluate the impact of different soil types and nitrogen doses on the yield of flower heads of A. chamissonis cultivated in eastern Poland and (ii) to determine the concentration and yield of sesquiterpene lactones, flavonoids, and essential oils in the flower heads. These main groups of secondary metabolites characteristic for this herb species are important for using the raw material in the pharmaceutical and cosmetic industries.
The results of this study have extended the knowledge of the role of nitrogen in flower head yields in this species on the one hand. Moreover, they have shown the effect of nitrogen on the content and yield of sesquiterpene lactones, flavonoids, and essential oils in flower heads on the other hand. This highlights the role of nitrogen doses and the soil type as an environmental aspect [72]. The research results have supported the cultivation of A. chamissonis in Central Europe in sustainable environmental conditions allowing the optimal use of nitrogen to minimize its migration to the environment and prevent habitat eutrophication, which occurs when excessive doses of this macronutrient are applied.

Plant Morphology
The meadow arnica has a long (up to 30 cm), thick (1.5-2.5 mm), unbranched, light brown, bare rhizome with numerous adventitious roots. In spring each year, it produces rosettes of lanceolate-ovoid leaves and 20-90 cm high inflorescence shoots branched in the upper part. The stem bears 5-10 pairs of 2-4 cm wide and 10-15 cm long leaves, decreasing in their sizes towards the apex. The tops of the branching shoots of the meadow arnica bear 3 to 15 flower heads. The light-yellow flowers are gathered in 4 cm diameter heads [1,3,4]. The flower heads are composed of numerous small disc florets surrounded by less numerous longer (usually 15-18 mm) and shallowly dentate ray florets [1,77]. In Poland, the flowering of the species usually begins at the turn of May and June and lasts for approximately three weeks [7].

Experimental Conditions
The field experiment was carried out in two-, three-, and four-year-old arnica plantations in 2017-2019. The field experiment was established in the second ten days of April 2016. The field was deeply plowed after harvesting the forecrop (white mustard Sinapis alba L.) in autumn 2015. The next spring, a cultivator was used for soil tillage and leveling before setting up the experiment. Runners with 4 to 6 leaves and a well-developed 10 cm long underground rhizome were planted in the experimental plots. Immediately after transfer from the original plantation site (collection of the Department of Industrial and Medicinal Plants, University of Life Sciences in Lublin), the runners were planted into previously prepared furrows. They were planted at an 8-10 cm depth with 20 cm spacing and covered with soil. The 40 cm row spacing resulted in the density of 125,000 plants·ha −1 .
The first year of the experiment (2016) was regarded as a preliminary year (no nitrogen fertilization was used), due to the low yields of flower heads, as the plants produced runners and roots at the expense of the aboveground parts. Moreover, due to the poorly developed root system, there was a high probability in the first weeks after planting that the introduced nitrogen fertilizers would not be fully absorbed by the plants. The following years 2017-2019, were regarded as the production years and were presented in a factorial system.
The research was conducted in two locations in the eastern part of Poland, differing in soil conditions. The first experimental plot (51 • 31 25 N; 22 • 45 04 E) was located on podzolic soil-sandy soil (SS) characterized by moderate content of organic matter and phosphorus, low potassium content, very low magnesium levels, and very acidic pH. The other part of the experiment was located on a plot (51 • 29 28 N; 22 • 51 18 E) with brown soil-loamy soil (LS) with high content of organic matter, very high content of phosphorus, moderate potassium and magnesium levels, and a neutral pH value (Table 1). The experiment was set up on 12 m 2 plots using the randomized block method with three replications. In the experiment (both soils), five levels of nitrogen fertilization were applied: 0, 30, 60, 90, and 120 kg N per ha. Nitrogen (34% ammonium nitrate) was used in two doses: After the beginning of plant vegetation in spring and in the phase of inflorescence shoot formation. The same fertilization with phosphorus (single superphosphate 80 kg P·ha −1 ) and potassium (potassium salt 120 kg K·ha −1 ) was applied in both plots in autumn.
The plants were weeded manually during the experiment. No diseases and pests of meadow arnica were found during the vegetation period; therefore, no plant protection agents were used.

Plant Material
Arnica chamissonis flower heads were collected successively from the entire plot in the full flowering phase (ray florets open; half of the disc florets open) in the same experimental units in the following years-2017-2019. After each harvest, fresh flower heads were weighed and dried in a drying room at 40 • C. After drying, the samples were weighed to determine the dry weight of the raw material. Next, the Arnicae anthodium raw material intended for laboratory analyses was stored in paper bags at room temperature.

Chemical Analyses 2.4.1. Sesquiterpene Lactones
Sesquiterpene lactones (SLs) were determined with the chromatographic method described in Polish Pharmacopoeia VIII [78]. The results are presented as % of the sum of sesquiterpene lactones calculated as dihydrohelenalin tiglinate equivalents.

Flavonoids
The content of flavonoids (Fs) expressed as quercetin equivalents were determined with the spectrophotometric method specified by Polish Pharmacopoeia VI [79].

Essential Oil
The percentage of essential oils (EO) was determined with the hydrodistillation method described in Polish Pharmacopoeia VI [79].
All chemical analyses were performed in triplicate.

Theoretical Yield of Metabolites per Unit Area
The yield of metabolites (SLs, Fs, EO) was expressed in kg·ha −1 . The results were used to calculate the yield of SLs, Fs, and EO per 1 ha (yield of flower heads × content of metabolites).

Statistical Analysis
The multi-way analysis of variance (ANOVA) and subsequent Tukey tests were used. The differences were considered significant at p < 0.05. Pearson's correlation coefficients were calculated to evaluate the significance of the relationships between the variables. The statistical analyses were carried out using the Statistica 6.0 software. Variation in the relationship between the studied characteristics was explored using principal component analysis (PCA). PCA is a common technique used to describe patterns of variation within multi-dimensional datasets [80]. Before analysis, all data were centered and logtransformed. The analyses were carried out using the statistical package (MVSP) program version 3.1. [81].  The thermal conditions fluctuated considerably during the study period (2017-2019) ( Figure 1). The mean annual air temperature in this period was 9.2 • C, which was 1.5 • C higher than the multiyear mean . The highest average annual air temperature (9.8 • C) was recorded in the third study year, i.e., 2019. In turn, 2017 had a mean temperature of 8.4 • C, which was the coldest year. Plant growth and yields are largely dependent on the weather conditions prevailing during the growing season. The mean temperature in the vegetation period (April-October) in the years of flower head harvesting (2017-2019) was 15.1 • C, which was 1.7 • C higher than the multiyear mean. The highest mean temperature during the growing season was recorded in the second study year (2018); it was 1.1 • C higher than the mean value in 1965-2015. The lowest mean temperature during the growing season was recorded in 2017; the mean temperature (14.0 • C) was 1.1 • C lower than the multiyear value.

Meteorological Conditions
The mean annual precipitation rate during the study (2017-2019) was 540.5 mm, and this value was 50.7 mm lower than the multiyear mean value (Figure 1). The lowest amount of precipitation was recorded in 2018 (the second year of the study), characterized by an over 112 mm decline in its rate in comparison with the mean value for 1965-2015. In the years of flower head harvesting, the vegetation seasons (April-October) were characterized by very diverse precipitation sums. The highest rainfall rate during the growing season was recorded in 2017 (448.4 mm), while 2018 and 2019 were characterized by similar values, i.e., 332.3 and 346.4 mm, respectively (by 89.9 and 75.8 lower than the multiyear sum).
The thermal and moisture conditions during meadow arnica flowering are important for the yield and quality of the raw material. The meadow arnica in Poland flowers from the end of May to the end of the second ten days of June [7]. The weather conditions in this phase varied during the three-year study period but were generally favorable and ensured high yields of flower heads. The warm and moderately humid year 2017 turned out to be the most favorable for arnica plant growth and development. It was characterized by an appropriate distribution of precipitation during the period of intensive plant growth (April, May); hence, the weather conditions contributed to the high yields of the raw material.

Results and Discussion
The results of the statistical analysis showed a statistically significant main effect of the soil type (S), nitrogen fertilization (N), and years (Y) on the content of all analyzed metabolites. In the case of the studied chemical compounds, a statistically significant effect of the S × N interactions, S × Y interactions, and N × Y interactions on all analyzed metabolites was confirmed. An effect of the S × N × Y interactions on sesquiterpene lactones and flavonoids was found ( Table 2). As shown by literature data, the yield of meadow arnica flower heads (YFH) in eastern Poland varies widely from approximately 404 to 1300 kg·ha -1 [37,82]. In the present study, a significantly higher yield of flower heads was obtained in the loamy soil than in the sandy soil variants (1310.3 and 1029.5 kg·ha −1 , respectively). The yields were higher than values reported in previous studies [37,82], and their magnitude was significantly influenced by the experimental factors (Table 2, Figure 2). The largest yield of Arnicae anthodium  In both soil variants, the highest yield of flower heads was obtained in the second year of cultivation (2017); it was in the range from 1225 to 1956 kg·ha −1 in the loamy soil variants and from 995 to 1518 kg·ha −1 in the sandy soil (lower by 19.3%). In the third year of cultivation, the value of the yield of flower heads was lower by 6.1 and 14.0%, respectively. In the fourth year, the yields decreased significantly (by 17.6 and 45.4%, respectively, compared to the year with the highest yields), which was especially noticeable in the sandy soil variants (Figure 2). Similar results were presented by other authors [37,82,83].
The nitrogen fertilization applied in all the study years increased the yield of flower heads (Table 2, Figure 2). In the second and third years, the meadow arnica cultivated in the sandy soil variants had significantly higher yields in plots fertilized with 60 kg N·ha −1 , compared to the control. In the loamy soil plots, the increase in the N dose from 0 to 90 kg·ha −1 elevated the flower head yield in the two-year-old plants (2017). In turn, the yields increased significantly up to the dose of 60 kg N·ha −1 in the third year of cultivation. In the fourth year of plant growth on both soils, a significant increase in the yield was obtained after applying 90 kg N·ha −1 in comparison with the control. The higher amounts of nitrogen (90 and 120 kg N·ha −1 ) used in the present study caused an increase in the yield of flower heads, but the differences in the yields were not confirmed statistically ( Figure 2).
Literature data report an increase in the weight of flower heads produced by Chamomilla recutita (L.) Rausch. and Chrysanthemum indicum L. with increasing nitrogen doses [67,69,84]. As shown by Giannoulis et al. [84], using 80 and 160 kg N·ha −1 in experimental plots contributed to a significant increase in the yield of chamomile flower heads compared to the nitrogen-unfertilized control. Nitrogen fertilization was found to exert a positive effect on the yields of other medicinal species from the family Asteraceae. In Tagetes erecta L., a two-fold increase in the nitrogen dose (from 100 to 200 kg N·ha −1 ) contributed to an over 30% increase in the inflorescence yield [85]. In turn, Orange King and Tokaj cultivars of the marigold produced on average 12% and 9% higher weight of flower heads with each additional dose of 40 kg N·ha −1 [57,58].
The type of soil and nitrogen fertilization significantly modified the percentage content of sesquiterpene lactones in the analyzed material (Table 2). Their content in the meadow arnica flower heads ranged from 0.31 to 0.51% in the sandy soil variants and from 0.32 to 0.56% in the loamy soil ( Figure 3). In most cases, the flower heads obtained in the present experiment met the Polish Pharmacopoeia XI [86] standards for Arnicae flos, i.e., the minimum content of sesquiterpene lactones of 0.40%. These standards were met by the flower heads harvested from the four-(2019) and three-year-old (2018) plants growing on both soils fertilized with 60-120 kg N·ha −1 .
The highest content of sesquiterpene lactones was detected in meadow arnica flower heads harvested from the three-(2018) and four-year-old (2019) plants growing on loamy soil fertilized with the highest nitrogen dose of 120 kg N·ha −1 (0.56 and 0.55%, respectively). In turn, the lowest content of the compounds was determined in the flower heads from the two-year-old control plants (2017) grown on the sandy soil (0.31%). In both soil variants, the lowest content of sesquiterpene lactones was found in the raw material collected from the two-year-old plants (2017), regardless of nitrogen fertilization. The raw material harvested from plants grown in the loamy soil variants was characterized by a significantly higher concentration of sesquiterpene lactones compared to plants growing on the sandy soil (Table 2, Figure 3). A similar level of sesquiterpene lactones was reported by Olesińska and Sugier [37], whereas substantially lower contents were demonstrated by Ivanova et al. [87] and Kimel et al. [27]. In both soil variants, the content of sesquiterpene lactones in the flower heads was significantly higher in the plots fertilized with 90 and 120 kg N·ha −1 compared to the control (Table 2, Figure 3). There are no available literature reports on the effect of nitrogen fertilization on the content of sesquiterpene lactones in the meadow arnica. In other herbal plants, the information on the impact of nitrogen on the content of sesquiterpene lactones in the raw material is not explicit. Kim and Lee [69] reported a nitrogen fertilization-induced decrease in the concentration of one of the sesquiterpene lactones in Chrysanthemum indicum flowers from 0.87 to 0.75 g cumambrin A kg·d·w −1 (fertilization with 0 and 300 kg N·ha −1 , respectively). Increasing doses of nitrogen stimulated Artemisia annua L. plants to biosynthesize nearly 50% higher amounts of artemisinin [88].
The content of flavonoids in the meadow arnica flower heads was correlated significantly with the experimental factors (Table 2, Figure 4). The percent content of flavonoids in the raw material ranged from 0.41 to 0.51% in the sandy soil variants and from 0.38 to 0.57% in the loamy soil ( Figure 4). The highest content of flavonoids was found in the flower heads of the three-year-old plants (2018) growing on the loamy soil fertilized with 60 and 90 kg N·ha −1 (0.56 and 0.57%, respectively). In turn, the lowest concentration of flavonoids was detected in the flower heads of the two-year-old plants (2017) growing in the loamy soil control plot (0.38%). The present study showed a significant effect of nitrogen fertilization on the flavonoid content in the flower heads. In both soil variants, a significantly higher level of flavonoids in the flower heads, compared to the control, was obtained after applying 60 kg N·ha −1 throughout the study. A further increase in the nitrogen dose (up to 90 and 120 kg N·ha −1 ) did not influence the flavonoid content in the flower heads. In turn, in the case of the two-year-old plants grown on the sandy soil and the three-year-old plants grown on the loamy soil, the increase resulted in a decline in the concentration of this metabolite in the flower heads ( Figure 4). As shown by literature data, the content of flavonoids in meadow arnica raw material ranges from 0.11 to 0.76% [37,82,89], which indicates agreement of the results of the present study with this range. In sesquiterpene lactones, there are no available literature reports on the effect of nitrogen fertilization on the content of flavonoids in the meadow arnica. Similar findings of the effect of nitrogen fertilization on the flavonoid content have been described in a study of summer savory (Satureja hortensis L.), where 40 kg·ha −1 was the most favorable nitrogen dose [90]. There is no clear information on the impact of nitrogen on the content of flavonoids in herb plants from the family Asteraceae. Tshivhandekano et al. [91] reported that increasing nitrogen doses stimulated the synthesis of flavonoid compounds in Athrixia phylicoides DC., with the highest increase in quercetin content induced by using 75 kg N·ha −1 . A further increase in nitrogen doses led to a significant decrease in the concentration of flavonoids, as in the case of the meadow arnica. In turn, it was shown that marigold flower heads contained the highest content of flavonoids in control plots [57,58].
The content of essential oils in the meadow arnica flower heads analyzed in the present study ranged from 0.14 to 0.19% (Figure 5), which agrees with other literature results [10,37]. In flavonoids and sesquiterpene lactones, significantly higher content of essential oils was determined in the flower heads of plants grown in the loamy soil variants, which is consistent with literature data [10]. In sesquiterpene lactones, the flower heads of the four-year-old plants had the highest content of essential oils (2019). Nitrogen fertilization significantly modified their content in the raw material. In all study years, a significant increase in the essential oil content in the flower heads collected from the loamy soil variants, compared to the control, was noted after applying 60 kg N·ha −1 . No significant increase in the essential oil content was noted after fertilization with the higher N doses (90 and 120 kg N·ha −1 ). Similar relationships were found for the two-and four-year-old plants in the sandy soil variants. In turn, in the third year of cultivation, a significant increase in the essential oil content was achieved after applying 90 kg N·ha −1 ( Figure 5). Literature data indicate that nitrogen fertilization affects the content of essential oils in various raw materials. A beneficial effect of nitrogen fertilization was demonstrated in the cases of summer savory [51,90], marigold [57,58], lemon verbena (Lippia citriodora Kunth.) [53], rosemary (Rosmarinus officinalis L.) [54], and sage (Salvia officinalis L.) [52]. Chamomile plants exhibited ambiguous responses to increasing nitrogen doses. Foladvand et al. [55] reported that an increase in the nitrogen dose to 100 kg·ha −1 caused a 16.5% decrease in the essential oil concentration, compared to raw material from the control plot. Different data were presented by Giannoulis et al. [84], who noted an increase in the essential oil content with increasing nitrogen doses, i.e., chamomile flower heads obtained from plots fertilized with 160 kg N·ha −1 contained 15.6% higher levels of essential oils than plants from the control plot.
The yield of sesquiterpene lactones, flavonoids, and essential oils per 1 ha varied significantly depending on the experimental factors ( Table 3). The most favorable results of the yield of the active substances were obtained in the meadow arnica cultivated in the loamy soil variants. During the three study years, plants cultivated in this soil produced on average 5.63 kg of sesquiterpene lactones, 6.06 kg of flavonoids, and 2.28 kg of essential oils. In turn, 4.18 kg of sesquiterpene lactones, 4.64 kg of flavonoids, and 1.67 kg of essential oils were obtained in the sandy soil variants (Table 3). Literature reports indicate lower yields of these metabolites, i.e., approx. 3.7 kg of sesquiterpene lactones, 4.4 kg of flavonoids, and 1.7 kg of essential oils · ha −1 [10,37]. The present study showed a significant effect of nitrogen fertilization on the yield of sesquiterpene lactones, flavonoids, and essential oils. High values were obtained in the yields of sesquiterpene lactones and essential oils at the doses of 90-120 kg N·ha −1 and the yield of flavonoids at the doses of 60-90 N·ha −1 .   Figure 6 shows the results of the PCA ordination of the samples. The eigenvalues of the first (4.28) and second (1.94) axes indicate the presence of two gradients, within which the samples are differentiated in terms of the examined traits. The first two axes explain 97.72% of the variability (61.17% the first axis, 27.65% the second axis), which proves that the traits correlated with these axes are greatly important for interpreting the differentiation and correlations between these traits. The flavonoid content, yield of sesquiterpene lactones, yield of essential oils, yield of flavonoids, and yield of flower heads are clearly correlated with the first axis. The content of sesquiterpene lactones and essential oils is positively correlated with the second axis. The first axis presents the gradient of flavonoids, yield of sesquiterpene lactones, yield of essential oils, yield of flavonoids, and yield of flower heads, which implies that the value of these traits increases with the increasing nitrogen doses in both soil variants. The variation between the study years is evident as well. The highest flavonoid content and yield of sesquiterpene lactones were exhibited by samples collected in 2018 from the loamy soil ( Figure 6). In turn, sesquiterpene lactones and essential oils were positively correlated with the second axis and with each other in the sandy soil and loamy soil variants (Tables 4 and 5). This indicates that the content of these compounds increased in the subsequent years and with the increase in nitrogen doses. The yield of sesquiterpene lactones and the yield of essential oils did not depend on the concentration of sesquiterpene lactones and essential oils but correlated with the increase in the yield of flower heads of plants from the sandy soil and loamy soil variants. In turn, the yield of flavonoids was influenced by both the concentration of flavonoids and the yield of flower heads (Tables 4 and 5).

Conclusions
The meadow arnica is a relatively new species in field cultivation; hence, the research on the influence of agronomic factors on its yield and the content of the main active substances is incomplete and requires supplementation. The present study showed that both the soil type and the nitrogen fertilization had a significant effect on the raw material yield, as well as the content and yield of the main active substances. The cultivation of A. chamissonis on the loamy soil proved more beneficial, as higher flower head yields, higher levels of sesquiterpene lactones, flavonoids, and essential oils, and consequently higher yields of these metabolites were achieved. In turn, the varying levels of nitrogen fertilization can be considered an appropriate way to increase the production of Arnicae anthodium (the highest yields were obtained at doses of 60-90 kg N·ha −1 ) and modify the chemical composition of this raw material. The content of sesquiterpene lactones and essential oils increased in the subsequent years, along with the increase in nitrogen doses. In turn, the highest content of flavonoids was detected in the flower heads of the three-year-old plants. The yields of sesquiterpene lactones and essential oils did not depend on the concentration of metabolites, but positively correlated with the yield of flower heads in both sandy and loamy soils. In turn, the yield of flavonoids was modified by the concentration of flavonoids and the yield of flower heads.
As shown in the present study, the yield of flower heads and the yields of metabolites of this medicinal plant species indicate favorable climatic and edaphic conditions for cultivation in eastern Poland, where A. chamissonis is currently not cultivated. Nevertheless, given the increasing demand for Arnicae anthodium in recent year, both the more fertile loamy soils and the poorer sandy soils can be used for the cultivation of the species. Moreover, a wide range of pharmacologically active substances (sesquiterpene lactones, flavonoids, essential oils) can be produced in this region of Europe by producing raw materials in sustainable conditions to meet the demands of the herb market.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author. The data are not publicly available due to ongoing unpublished research.

Conflicts of Interest:
The authors declare no conflict of interest.