Effect of Plant-Growth-Promoting Fungi on Eggplant ( Solanum melongena L.) in New Reclamation Land

: Land reclamation may expand the supply of usable land for food security. Immature soil is not suitable for plant growth and needs to be amended by the addition of organic matter and plant growth-promoting (PGP) microorganisms. However, the effects of different PGP fungi on plant growth in immature soil are largely unexplored. In order to obtain beneﬁcial soil microorganisms with a good PGP ability in new reclamation land, 162 fungal isolates were isolated from different abandoned wastelands, four isolates of which were obtained in this study by the screening of P solubilization, siderophore production, and indole acetic acid (IAA) production. The result of this study revealed that isolate HZ123 had the highest ability to solubilize P and produce siderophores and IAA, followed by HZ23, HZ10, and HZ06. Based on the results of morphological and molecular analyses, isolate HZ06 was identiﬁed as Penicillium oxalicum , isolates HZ23 and HZ10 were identiﬁed as Aspergillus brunneoviolaceus , and isolate HZ123 was identiﬁed as Aspergillus tubingensis . Further-more, the results of in vivo PGP assays demonstrated that isolate HZ123 has a minimal negative effect on the growth of eggplant; however, the other three isolates, particularly isolate HZ06, caused the greatest increase in eggplant biomasses. Overall, these results indicate that isolate HZ06 has great potential as a PGP fungus to develop biofertilizer for application in eggplant production in immature soil from new reclamation land.


Introduction
China is the world's most populous country, and, as such, food security is a huge problem currently facing the country. With the development of urbanization and the decrease in available land resources, expanding the utilization of land resources has become an important task to ensure food security in Hangzhou city, Zhejiang province, China. In order to expand the available land supply, the barren mountain land and forest land in the mountainous areas of Hangzhou, Zhejiang Province, China, were reclaimed for agricultural use. The immature soil in developed land is not suitable for plant growth because it does not have enough time to develop via normal soil-forming processes. In most instances, the soil is acidic and poor in nutrients with a high gravel content [1].
In recent years, eggplant cultivation in mountainous areas has been well developed in Zhejiang Province, especially in Hangzhou, due to its various advantages. During the growth of eggplant, organic amendments, such as livestock manure, biosolids, pulp byproducts, wood residuals, and crop residues, are widely used in soil reclamation, which can modify soil physical and chemical properties and exert a great influence on heterotrophic microbial communities [2]. Moreover, soil microorganisms have played an important role

Soil Sample Collection and Fungal Isolation
In order to isolate PGP fungi, nine soil samples were collected in November 2019 from immature soil without plants in wastelands that had been abandoned for many years. The wastelands were located in a mountainous rural area of Yaolin town, Tonglu country (119 • 20 56 E, 29 • 50 7 N), Zhejiang Province, China. The soils have a pH of 5.03, with 0.072% of total N, 2.42 mg/kg of available P, and 61.57 mg/kg of available K, while the soil type is acrisols (acidic soils with a layer of clay accumulation), according to the soil classification system of FAO-UNESCO. The soils were sampled from a 5-20 cm soil layer. Each sample was placed into a sterilized polythene bag and then stored in a refrigerator at 4 • C. Fungal isolation was carried out by preparing a series of 10-fold dilutions of soil suspension from each sample and then inoculating 200 µL aliquots on potato dextrose agar (PDA) medium (potato extract 200 g, dextrose 20 g, agar 20 g in 1000 mL ddH 2 O), containing 100 µg·mL −1 of ampicillin and 50 µg·mL −1 of streptomycin sulphate. The plates were incubated at 25 • C for 3-5 days. The different colonies were chosen based on their morphological characteristics, and then each selected colony was purified via single-spore isolation. The purified isolates were stored in 1.5 mL tubes with 20% glycerol at −70 • C.

Determination of Phosphate-Solubilizing Ability
The 162 fungal isolates were inoculated on a modified NBRI-MTSB medium (glucose 10 g, MgCl 2 5 g, KCl 0.2 g, MgSO 4 ·7H 2 O 0.25 g, (NH 4 ) 2 SO 4 0.1 g, Ca 3 (PO 4 ) 2 5 g, agar 15 g in 1000 mL ddH 2 O; medium was adjusted to 7.0). Following the incubation at 25 • C for 3 days, the phosphate (P) solubility was determined as described in [12,13], by measuring the size of the fungal phosphate-solubilizing zone. This experiment was repeated twice with six replicates of each isolate.

Determination of Siderophore Production
In order to evaluate the isolates' ability to produce siderophores, the 162 isolates were grown on chrome azurol sulphonate (CAS) agar plates [14], in which 10 × MM9 salts were prepared according to the method described by [15] in iron-depleted chemically-defined minimal medium (MM9). The prepared CAS agar plates were inoculated with the fungal isolates and then incubated at 25 • C for 3 days. The production of the siderophore by the fungal isolates was determined by measuring the visible orange halos around colonies on media. According to their abilities of phosphate solubilization and siderophore production, specific fungal isolates were selected for further evaluation.

Indole Acetic Acid (IAA) Detection
In order to determine the effect of the selected isolates in the conversion of tryptophan to IAA under different pH conditions, the fungal isolates were grown on PDA. After incubation at 25 • C for 3 days, the pieces (5 mm in diameter) containing hyphae were cut from the colony margin of each isolate and placed in glass test tubes containing 10 mL of PDB with different concentrations of tryptophan (0.1, 0.5, 1, and 1.5%) and different pH values (pH 5.0, 7.0, and 9.0). The tubes were incubated at 28 • C in a rotary shaker at 150 rpm for 7 days. After centrifugation at 8000 rpm for 5 min, 1 mL of supernatant from each tube was mixed with 4 mL Salkowski's reagent (0.5 mol/L FeCl 3 15 mL, H 2 SO 4 300 mL, ddH 2 O 500 mL) [16], and then the mixture was incubated in the dark at room temperature for 30 min. IAA production was able to be determined by observing the development of pink color. Furthermore, IAA concentration was calculated using a standard curve, which was prepared by measuring the optical density of standard IAA (0-100 µg mL −1 ) at a wavelength of 530 nm with a UV-Vis spectrophotometer (Perkin Elmer Lambda 35, Waltham, MA, USA) [17]. The amount of the produced IAA was expressed in micrograms for IAA secreted per unit of optical density.

Morphological Identification
For morphological observation, the screened single-spore isolates were grown on Czapek yeast autolysate (CYA) agar, malt extract autolysate (MEA) agar, and creatine agar (CREA) [18]. In detail, the isolates were inoculated at three points on each plate of each medium and incubated at 25 and 37 • C in the dark for 7 days. Following washing with 70% ethanol, excess conidia were used for morphological observation. The fungal asexual structures were observed and measured using a Zeiss Axiophot 2 microscopy with Axiocam CCD camera and AxioVision digital imaging software (AxioVision Software Release 3.1., v.3-2002; Carl Zeiss Vision Imaging Systems, Zeiss, Jena, Germany).

Phylogenetic Analysis
After obtaining the results of morphological identification, phylogenic analysis of Penicillium and Aspergillus spp. was carried out using the four-locus combined ITS region, TUB, RPB2, and CaM gene dataset (Supplementary Materials Table S1). For Penicillium spp., we selected the best TIM2 + I + G or TIM2 + I + G model for MP or BIfor ITS; TrNef + I + G or TrNef + I + G for CMD; TIM1 + I + G or TIM2ef + I + G for RPB2; and TIM1 + I + G or TrNef + G for TUB.

Effect of Isolates on Plant Growth Promotion in New Reclamation Land
The PGP effects of the four selected isolates were evaluated by applying them to the seedlings of the eggplant cv. Hangqie 2010 (an eggplant F1 hybrid bred by the Vegetable Institution, Hangzhou Academy of Agricultural Sciences). In brief, the seeds were sterilized with 2% sodium hypochlorite solution for 2 min, rinsed three times with sterile water, soaked in sterile water for 5 h, and then air dried in a laminar flow hood. Subsequently, they were placed onto the surfaces of the wet sterilized filter papers and incubated at 25 • C for 3 days. The germinated seeds were sown into the 32 cells of seed-growing trays containing cocopeat substrate (Bairun Agricultural Technology Co., Ltd., Hangzhou, China) and moved to a greenhouse with a relative humidity level of 70-80% and a temperature of 25 • C. Fungal conidia suspension for each isolate was prepared with sterile distilled water by suspending conidia harvested from 7-10-day-old cultures grown on PDA medium at 25 • C. The final concentration was determined using a hematocytometer. All the tested fungi had a concentration of 10 6 spores/mL. When plants grew to the four-leaf stage, they were transplanted into pots containing unsterilized soil from the new reclamation land with pH 5.0, and 10 mL of conidia suspension of each test isolate was poured into the soil around the plants and then incubated under the same conditions as described above. Eggplant inoculation with sterile water was used as the control. Thirty plants (n = 30) were used for each treatment, and three independent replicates were performed for each treatment. After growth for 30 days, eggplant seedlings were removed out from the pots and rinsed with tap water. The PGP ability of the fungal isolates was determined by measuring the seedlings' height, tap root length, and dry and fresh weights. Dry weights were measured by drying plant organs in an oven at 65 • C for 2 days. The growth promotion efficacy (GPE%) was assessed using the following formula: GPE% = (treatment − control)/control × 100%.

Statistical Analysis
Statistical analyses were carried out using SPSS software version 16 (SPSS, Chicago, IL, USA). The levels of significance (p < 0.05) of the main treatments and their interactions were calculated through an analysis of variance after testing for normality and variance homogeneity.

Fungal Isolation and Screening of Phosphate-Solubilizing Ability
The results of this study indicate that a total of 162 fungal isolates were obtained from nine soil samples. Furthermore, P solubilization assays showed that five isolates can produce a halo with a diameter >3.40 cm, indicating that these isolates have a great phosphate solubilization ability on the modified NBRI-MTSB media ( Figure 1). In detail, the diameter of the halo was 4.20, 3.88, 3.62, 3.60, and 3.48 cm for isolates HZ123, HZ23, HZ22, HZ10, and HZ06, respectively. Furthermore, isolate HZ123 produced the largest zone, which was significantly (p < 0.05) higher than that of the other four isolates. In addition, there was no difference among the isolates HZ22, HZ10, and HZ06. This result suggested that the five isolates can be used to develop P solubilization agents.

Fungal Isolation and Screening of Phosphate-Solubilizing Ability
The results of this study indicate that a total of 162 fungal isolates were obtained from nine soil samples. Furthermore, P solubilization assays showed that five isolates can produce a halo with a diameter >3.40 cm, indicating that these isolates have a great phosphate solubilization ability on the modified NBRI-MTSB media ( Figure 1). In detail, the diameter of the halo was 4.20, 3.88, 3.62, 3.60, and 3.48 cm for isolates HZ123, HZ23, HZ22, HZ10, and HZ06, respectively. Furthermore, isolate HZ123 produced the largest zone, which was significantly (p < 0.05) higher than that of the other four isolates. In addition, there was no difference among the isolates HZ22, HZ10, and HZ06. This result suggested that the five isolates can be used to develop P solubilization agents.

Siderophore Production
The results of this study indicate that around 82% of all isolates were able to produce visible orange halos with different sizes around fungal colonies on CAS media after 3 days of incubation, revealing the production of siderophore. Among the above-mentioned five P-solubilizing isolates, no halo was observed in isolate HZ22, but clear halos were found in the other four isolates. The diameters of the halos were 2.78, 2.05, 1.23, and 1.04 cm for isolates HZ123, HZ06, HZ23, and HZ10, respectively. Furthermore, isolate HZ123 exhibited the largest halo diameter, which was significantly greater than that of the other three isolates ( Figure 2). In addition, there was no significant difference in halo diameter between isolates HZ23 and HZ10. According to the abilities of phosphate solubilization and siderophore production, four isolates were selected for further analysis.

Indole Acetic Acid (IAA) Detection
The results of this study indicate that the four isolates differed in IAA production in the medium amended with different concentrations of tryptophan at different pH values. Indeed, isolate HZ123 exhibited the maximum production of IAA in the medium, amended with 1.5% tryptophan at pH 7.0 with the concentration of 267.38 μg/mL, while no significant (p < 0.05) difference was found in IAA production between 1.5% tryptophan at pH 7.0 and 1.0% tryptophan at pH 5.0 (238.75 μg/mL) or 1.0% tryptophan at pH 7.0 (224.58 μg/mL). Furthermore, isolate HZ10 exhibited strong production of IAA with the concentrations of 85.33 and 78.67 μg/mL in the medium with 1.5 and 1.0% tryptophan, respectively, at pH 9.0. In addition, isolates HZ06 and HZ23 exhibited weak production of IAA with the concentration of 73.92 and 71.50 μg/mL, respectively, in the medium with

Siderophore Production
The results of this study indicate that around 82% of all isolates were able to produce visible orange halos with different sizes around fungal colonies on CAS media after 3 days of incubation, revealing the production of siderophore. Among the above-mentioned five P-solubilizing isolates, no halo was observed in isolate HZ22, but clear halos were found in the other four isolates. The diameters of the halos were 2.78, 2.05, 1.23, and 1.04 cm for isolates HZ123, HZ06, HZ23, and HZ10, respectively. Furthermore, isolate HZ123 exhibited the largest halo diameter, which was significantly greater than that of the other three isolates ( Figure 2). In addition, there was no significant difference in halo diameter between isolates HZ23 and HZ10. According to the abilities of phosphate solubilization and siderophore production, four isolates were selected for further analysis.

Fungal Isolation and Screening of Phosphate-Solubilizing Ability
The results of this study indicate that a total of 162 fungal isolates were obtained from nine soil samples. Furthermore, P solubilization assays showed that five isolates can produce a halo with a diameter >3.40 cm, indicating that these isolates have a great phosphate solubilization ability on the modified NBRI-MTSB media ( Figure 1). In detail, the diameter of the halo was 4.20, 3.88, 3.62, 3.60, and 3.48 cm for isolates HZ123, HZ23, HZ22, HZ10, and HZ06, respectively. Furthermore, isolate HZ123 produced the largest zone, which was significantly (p < 0.05) higher than that of the other four isolates. In addition, there was no difference among the isolates HZ22, HZ10, and HZ06. This result suggested that the five isolates can be used to develop P solubilization agents.

Siderophore Production
The results of this study indicate that around 82% of all isolates were able to produce visible orange halos with different sizes around fungal colonies on CAS media after 3 days of incubation, revealing the production of siderophore. Among the above-mentioned five P-solubilizing isolates, no halo was observed in isolate HZ22, but clear halos were found in the other four isolates. The diameters of the halos were 2.78, 2.05, 1.23, and 1.04 cm for isolates HZ123, HZ06, HZ23, and HZ10, respectively. Furthermore, isolate HZ123 exhibited the largest halo diameter, which was significantly greater than that of the other three isolates ( Figure 2). In addition, there was no significant difference in halo diameter between isolates HZ23 and HZ10. According to the abilities of phosphate solubilization and siderophore production, four isolates were selected for further analysis.

Indole Acetic Acid (IAA) Detection
The results of this study indicate that the four isolates differed in IAA production in the medium amended with different concentrations of tryptophan at different pH values. Indeed, isolate HZ123 exhibited the maximum production of IAA in the medium, amended with 1.5% tryptophan at pH 7.0 with the concentration of 267.38 μg/mL, while no significant (p < 0.05) difference was found in IAA production between 1.5% tryptophan at pH 7.0 and 1.0% tryptophan at pH 5.0 (238.75 μg/mL) or 1.0% tryptophan at pH 7.0 (224.58 μg/mL). Furthermore, isolate HZ10 exhibited strong production of IAA with the concentrations of 85.33 and 78.67 μg/mL in the medium with 1.5 and 1.0% tryptophan, respectively, at pH 9.0. In addition, isolates HZ06 and HZ23 exhibited weak production of IAA with the concentration of 73.92 and 71.50 μg/mL, respectively, in the medium with

Indole Acetic Acid (IAA) Detection
The results of this study indicate that the four isolates differed in IAA production in the medium amended with different concentrations of tryptophan at different pH values. Indeed, isolate HZ123 exhibited the maximum production of IAA in the medium, amended with 1.5% tryptophan at pH 7.0 with the concentration of 267.38 µg/mL, while no significant (p < 0.05) difference was found in IAA production between 1.5% tryptophan at pH 7.0 and 1.0% tryptophan at pH 5.0 (238.75 µg/mL) or 1.0% tryptophan at pH 7.0 (224.58 µg/mL). Furthermore, isolate HZ10 exhibited strong production of IAA with the concentrations of 85.33 and 78.67 µg/mL in the medium with 1.5 and 1.0% tryptophan, respectively, at pH 9.0. In addition, isolates HZ06 and HZ23 exhibited weak production of IAA with the concentration of 73.92 and 71.50 µg/mL, respectively, in the medium with 1.5% tryptophan at pH 9.0 and pH 5.0 ( Figure 3). These results show that IAA production depended on the isolates, pH, and concentrations of tryptophan in the medium. ture 2021, 11, x FOR PEER REVIEW 1.5% tryptophan at pH 9.0 and pH 5.0 ( Figure 3). These results show that IAA produ depended on the isolates, pH, and concentrations of tryptophan in the medium.

Morphological Identification
The results of this study indicate that the four isolates differed in colony diamet CYA, MEA, CREA, and CYA medium and in organic acid production (Table 1). Base the morphological characteristics of CYA, MEA, and CREA medium, the four funga lates were firstly divided into different genera. Indeed, isolate HZ06 was attributed t genus Penicillium, in which colonies were in shades of green, mostly consisting of a d felt of conidiophores, chains of single-celled conidia produced from the phialide. Fur more, isolates HZ23, HZ10, and HZ123 were attributed to the genus Aspergillus Se Nigri due to the dark brown to black conidia, with uniseriate or biseriate conidioph spherical vesicles, and hyaline or lightly pigmented hyphae near the apex [29,30].
In accordance with the description in [33], isolates HZ23 and HZ10 with similar characteristics were identified as Aspergillus brunneoviolaceus based on the colony growth patterns on different media after 7 days of incubation ( Figure 6). In detail, on CYA, conidial heads: brown to dark brown near black, velutinous to slightly floccose, white to buff mycelium. On MEA, conidial heads: brown; mycelium: white. On CREA, fungus displayed poor sporulation but commonly good to very good acid production at 25 • C. Fungal stipes: smooth, hyaline, or slightly pigmented; vesicles: globose to ellipsoidal, 32-51 µm diam; conidial heads: uniseriate; phialides: 7-9 × 3.5-4.5 µm, covering entire

Phylogenic Analysis of Sequence Data
Molecular phylogenies were constructed with ITS, CaM, RPB2, and TUB data of 36 taxa, while Talaromyces flavus CBS310.38 T was used as an outgroup. The alignments were 1974 characters, of which 154 were phylogenetically informative after removing the ambiguously aligned regions. The phylogenetic analysis showed that the topology of the best scoring ML tree analysis was congruent with the BI tree for the concatenated four-locus

Phylogenic Analysis of Sequence Data
Molecular phylogenies were constructed with ITS, CaM, RPB2, and TUB data of 36 taxa, while Talaromyces flavus CBS310.38 T was used as an outgroup. The alignments were 1974 characters, of which 154 were phylogenetically informative after removing the ambiguously aligned regions. The phylogenetic analysis showed that the topology of the best scoring ML tree analysis was congruent with the BI tree for the concatenated four-locus

Phylogenic Analysis of Sequence Data
Molecular phylogenies were constructed with ITS, CaM, RPB2, and TUB data of 36 taxa, while Talaromyces flavus CBS310.38 T was used as an outgroup. The alignments were 1974 characters, of which 154 were phylogenetically informative after removing the ambiguously aligned regions. The phylogenetic analysis showed that the topology of the best scoring ML tree analysis was congruent with the BI tree for the concatenated fourlocus dataset (Figure 7). The relationship between all reference isolates could be clearly distinguished on the level of species. The tested isolate HZ06 clustered with the isolates of P. oxalicum (5648, CV822, and CBS219.30 T ) as a distinct clade with high ML-BS (84%) and BI-PP (1.00) support. For Aspergillus spp., the best TIM2 + I + G or TIM2ef + I +G model for MP or BI was selected for ITS, TIM3+I+G or TIM3+I+G for CMD, TrN+I+G or TrNef+I+G for RPB2, and TIM1+G or TIM1+G for TUB. A phylogenic tree was constructed with ITS, CMD, RPB2, and TUB data of 52 taxa, while A. robstus NRRL6362 T was used as an outgroup. The alignments were 2392 characters after removing the ambiguously aligned regions, of which 279 were phylogenetically informative. The phylogenetic analyses showed that the two tested isolates, HZ10 and HZ23, clustered with the isolates of A. brunneoviolaceus (PW4049, PW4122, and NRRL4912) as a distinct clade with high ML-BS (86%) and BI-PP (1.00) support, while isolate HZ123 clustered with the isolates of A. tubingensis (NRRL62644, NRRL62643, NRRL62639, and NRRL4866) as a distinct clade with high ML-BS (100%) and BI-PP (1.00) support ( Figure 8).
In general, the results of this study indicate that morphological identification was identical to phylogenetic analysis. Therefore, it can be concluded that isolate HZ06 was identified as P. oxalicum, HZ123 was identified as A. tubingensis, and HZ23 and HZ10 were identified as A. brunneoviolaceus.

Effect of Fungal Isolates on Plant Growth Promotion
The results of PGP tests showed that the four isolates except HZ123 significantly promoted eggplant seedling growth at different levels under greenhouse conditions (Figure 9). Based on the phenotypic observation, inoculation of P. oxalicum HZ06 and A. brunneoviolaceus HZ23 and HZ10 caused an obvious increase in leaf size and root length compared to the control. Furthermore, the early flowering of eggplant was also affected by the isolates. Indeed, HZ06 blossomed abundantly after 27 days of colonization, while HZ10 occasionally blossomed after 29 days of colonization. However, HZ123 and HZ23 had not yet blossomed when harvested at 30 days ( Figure 9).
The measured data showed that the four isolates significantly (p < 0.05) affected the growth and biomass accumulation of eggplants compared to the controls (Table 2). Inoculation with P. oxalicum HZ06 resulted in an increase of 22.35%, 39.26%, and 47.69% in the length and dry and fresh weights of seedlings, respectively, and of 0.83%, 41.30%, and 28.72% in the length and dry and fresh weights of roots compared to the control. Furthermore, inoculation with A. brunneoviolaceus HZ23 caused a 12.61%, 28.15%, and 33.55% increase in the length and dry and fresh weights of seedlings, respectively, and a 1.99%, 28.26%, and 27.70% increase in the length and dry and fresh weights of roots, respectively, compared to the control. In addition, inoculation with A. brunneoviolaceus HZ10 resulted in a 6.61%, 23.70%, and 20.51% increase in the length and dry and fresh weights of seedlings, respectively, and a 8.12%, 43.48%, and 35.81% increase in the length and dry and fresh weights of roots, respectively, compared to the control. In contrast, A. tubingensis HZ123 exhibited a negative effect on the seedlings' dry and fresh weights (Figure 9). Table 2. Effect of four fungus strains on growth promotion of eggplant 30 days after inoculation. CMD, RPB2, and TUB data of 52 taxa, while A. robstus NRRL6362 T was used as an outgroup. The alignments were 2392 characters after removing the ambiguously aligned regions, of which 279 were phylogenetically informative. The phylogenetic analyses showed that the two tested isolates, HZ10 and HZ23, clustered with the isolates of A. brunneoviolaceus (PW4049, PW4122, and NRRL4912) as a distinct clade with high ML-BS (86%) and BI-PP (1.00) support, while isolate HZ123 clustered with the isolates of A. tubingensis (NRRL62644, NRRL62643, NRRL62639, and NRRL4866) as a distinct clade with high ML-BS (100%) and BI-PP (1.00) support ( Figure 8).

Effect of Fungal Isolates on Plant Growth Promotion
The results of PGP tests showed that the four isolates except HZ123 significantly promoted eggplant seedling growth at different levels under greenhouse conditions ( Figure  9). Based on the phenotypic observation, inoculation of P. oxalicum HZ06 and A. brunneoviolaceus HZ23 and HZ10 caused an obvious increase in leaf size and root length compared to the control. Furthermore, the early flowering of eggplant was also affected by th lates. Indeed, HZ06 blossomed abundantly after 27 days of colonization, while HZ casionally blossomed after 29 days of colonization. However, HZ123 and HZ23 h yet blossomed when harvested at 30 days (Figure 9).

Discussion
Immature soil is not suitable for plant growth, but it can be modified by adding organic matter and PGP microbes to promote soil maturity and plant growth [1][2][3]. However, little attention has been paid to the effect of PGP microbes on plant growth promotion in immature soil. In this study, the 162 fungal isolates were successfully isolated from the waste lands with poor nutrition. Among them, only four isolates, designated as HZ06, HZ23, HZ10, and HZ123, exhibited the ability to solubilize phosphate and produce siderophores and IAA. Furthermore, the four isolates HZ06, HZ23, HZ10, and HZ123 were identified as P. oxalicum, A. brunneoviolaceus, A. brunneoviolaceus, and A. tubingensis, respectively, based on both morphological observation and phylogenic analysis of sequence data.
As is well known, the effect of PGP microbes on various aspects of plant growth is often variable, depending largely on their successful survival, colonization, growth, and efficient adaptation to rapidly changing conditions. Interestingly, our previous study clearly showed that the growth of each strain in its original niche environment is significantly greater (p<0.01, Analysis of Variance (ANOVA) test) than that of the other strains under the same conditions, revealing the preference of each strain for a specific niche environment [34]. Therefore, it can be inferred that the four fungal isolates originating from wastelands may have greater potential to colonize in immature soil.
A considerable number of previous studies have been carried out with the aim of isolating and characterizing PGP fungi, including those belonging to the genera of Aspergillus and Penicillium [6,[35][36][37][38]. For example, Araujo et al. [35] showed that Aspergillus niger can promote the growth of coffee seedlings in a substrate with no P limitation. Javed et al. [36] reported the potential of endophytic fungus Aspergillus terreus as a potent plant growth promoter. Furthermore, Wakelin et al. [37] reported the effect of Penicillium fungi on plant growth and phosphorus mobilization in neutral-to-alkaline soils from southern Australia. Gomez-Munoz et al. [38] found that Penicillium bilaii inoculation resulted in increased root growth and, thus, nutrient uptake and plant growth of maize. In contrast with these findings, this study reported the plant growth-promoting effect of Aspergillus and Penicillium in new reclamation soil.
According to the results of previous publications, the plant growth promotion mechanism of PGP fungi, in particular, Aspergillus spp. and Penicillium spp., can be, at least partially, attributed to the fact that they have the capacity to enhance the solubilization of insoluble phosphate compounds [39,40] and produce IAA [41,42] and siderophore [43,44]. In agreement with these results, the four fungal isolates that were screened in this study all possessed the above-mentioned properties of PGP fungi. In contrast with previous reports, the results of this study also found that these isolates were able to produce organic acids on CREA medium. Many studies have indicated that the organic acids that are secreted by PGP fungi may affect plant growth [45,46]; therefore, further studies should be carried out to explore the role of the organic acids in plant promotion of Aspergillus spp. and Penicillium spp. in new reclamation soil.
Phosphorus is one of the most indispensable macronutrients for the growth and development of plants. It is well known that the microbial solubilization of inorganic phosphates has been attributed mainly in regard to the production of organic acids. For example, Vyas and Gulati [45] reported that 19 phosphate-solubilizing fluorescent Pseudomonas strains produced gluconic acid, oxalic acid, 2-ketogluconic acid, lactic acid, succinic acid, formic acid, citric acid, and malic acid in culture filtrates during the solubilization of inorganic phosphate. In agreement with this result, the four fungal isolates examined in the current study were able to solubilize P and produce organic acids. Interestingly, A. tubingensis HZ123 exhibited the best abilities of both P solubilization and organic acids production, while there was poor organic acid production in P. oxalicum HZ06, which had a weak ability of P solubilization. This indicates the correlation between P solubilization and organic acid production.
Furthermore, previous studies have also shown that the species P. oxalicum is tolerant to low and high pH for its survival [47], while soil amendment with A. tubingensis has been found to be able to enhance corn yield through its ability to dissolve phosphates into soil and reduce alkalinity in bauxite residues [48]. In contrast, in vivo PGP tests in this study indicated that the P. oxalicum HZ06 was a better PGP fungus than HZ123, although A. tubingensis HZ123 showed the best abilities of P solubilization and siderophore and IAA production among the four isolates in the in vitro experiments, while P. oxalicum HZ06 exhibited the worst abilities of P solubilization and siderophore and IAA production. Differences in results between in vitro and in vivo tests may be due to the biological nature of different fungal species.
In agreement with the result of this study, P. oxalicum has been reported to be able to utilize phosphate and contribute to soil fertilization [6,49]; several strains are patented as biofertilizers, commercial enzyme producers, or degraders of waste plant biomass and pollutants [31]. Furthermore, some studies indicate that P. oxalicum is a producer of natural products, including organic acids (oxalic, formic, tartaric, malic, acetic, and citric acids), various enzymes, mycotoxins, cytotoxic alkaloids, and even cytotoxic compounds [6,31], which may be a possible cause of the promoting effect of isolate HZ06 on the early flowering of the plant. On the other hand, our recent studies have indicated that different organic fertilizers can improve soil from newly reclaimed land to crop soil. Therefore, further studies should be carried to elucidate the plant growth-promoting mechanism of these fungal isolates, in particular HZ06, alone or in combination with organic amendments in new reclamation land.

Conclusions
In summary, our results successfully isolated and characterized the four PGP fungal isolates from wastelands via P solubilization and IAA and siderophore production. Based on the results of the morphological and molecular analyses, isolate HZ06 was identified as P. oxalicum, isolates HZ23 and HZ10 were identified as A. brunneoviolaceus, and isolate HZ123 was identified as A. tubingensis. The results of the in vivo plant growth test demonstrated that the fungal isolates, in particular isolate HZ06, have great potential as plant growthpromoting fungi to develop biofertilizer for application in eggplant production in immature soil from new reclamation land.