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Article

Pathogen-Induced Expression of OsDHODH1 Suggests Positive Regulation of Basal Defense Against Xanthomonas oryzae pv. oryzae in Rice

1
Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
2
National Laboratory of Seed Testing, National Seed Service, SENASEM, Ministry of Agriculture, Kinshasa 904 KIN1, Democratic Republic of the Congo
3
Department of Agriculture, Abdul Wali Khan University, Mardan 23200, Pakistan
4
Laboratory of Plant Molecular Breeding, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
*
Authors to whom correspondence should be addressed.
Agriculture 2020, 10(11), 573; https://doi.org/10.3390/agriculture10110573
Received: 15 October 2020 / Revised: 11 November 2020 / Accepted: 19 November 2020 / Published: 23 November 2020
(This article belongs to the Section Crop Protection, Diseases, Pest and Weeds)
Bacterial leaf blight (BLB), a vascular disease caused by Xanthomonasoryzae pv. oryzae (Xoo), induces a significant reduction in rice yield in severe epidemics. This study investigated the transcriptional regulation of the OsDHODH1 gene in rice cultivars exposed to the Xoo K3 isolate. The symptoms were monitored on a daily basis, and the lesion length of inoculated rice plants was scored 21 days post inoculation (dpi). The most resistant and the highly susceptible cultivars were used for gene expression analysis. The dihydroorotate dehydrogenase (DHODH) domain is shared by many proteins in different plant species, and in Arabidopsis, this protein is encoded by the AtPYD1 gene. To investigate the functional role of the OsDHODH1 gene under bacterial infection, we inoculated the Arabidopsispyd1-2 knockout (atpyd1-2) plants, lacking the AtPYD1 gene (orthologous gene of the rice OsDHODH1), with Pseudomonassyringae pv. tomato (Pst) DC3000 vir, and the phenotypic response was scored 9 dpi. Results show that OsDHODH1 was upregulated in Tunnae, the most resistant rice cultivar but downregulated in IRAT112, the highly susceptible rice cultivar. In addition, Tunnae, Sipi and NERICA-L14 exhibited a durable resistance phenotype towards Xoo K3 isolate 21 dpi. Moreover, the expression of OsPR1a and OsPR10b (the rice pathogenesis inducible genes) was significantly upregulated in Tunnae, while being suppressed in IRAT112. Furthermore, the atpyd1-2 plants exhibited a high susceptibility towards Pst DC3000 vir. AtPR1 and AtPR2 (the Arabidopsis pathogenesis inducible genes) transcripts decreased in the atpyd1-2 plants compared to Col-0 (wild type) plants. Due to the above, OsDHODH1 and AtPYD1 are suggested to be involved in the basal adaptive response mechanisms towards bacterial pathogen resistance in plants. View Full-Text
Keywords: bacterial leaf blight; Xanthomonas oryzae pv. oryzae; disease resistance; OsDHODH1; rice bacterial leaf blight; Xanthomonas oryzae pv. oryzae; disease resistance; OsDHODH1; rice
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MDPI and ACS Style

Rolly, N.K.; Imran, Q.M.; Kim, H.-H.; Aye, N.C.; Hussain, A.; Kim, K.-M.; Yun, B.-W. Pathogen-Induced Expression of OsDHODH1 Suggests Positive Regulation of Basal Defense Against Xanthomonas oryzae pv. oryzae in Rice. Agriculture 2020, 10, 573. https://doi.org/10.3390/agriculture10110573

AMA Style

Rolly NK, Imran QM, Kim H-H, Aye NC, Hussain A, Kim K-M, Yun B-W. Pathogen-Induced Expression of OsDHODH1 Suggests Positive Regulation of Basal Defense Against Xanthomonas oryzae pv. oryzae in Rice. Agriculture. 2020; 10(11):573. https://doi.org/10.3390/agriculture10110573

Chicago/Turabian Style

Rolly, Nkulu K., Qari M. Imran, Hyun-Ho Kim, Nay C. Aye, Adil Hussain, Kyung-Min Kim, and Byung-Wook Yun. 2020. "Pathogen-Induced Expression of OsDHODH1 Suggests Positive Regulation of Basal Defense Against Xanthomonas oryzae pv. oryzae in Rice" Agriculture 10, no. 11: 573. https://doi.org/10.3390/agriculture10110573

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