Physical Characterization of Mouse Deep Vein Thrombosis Derived Microparticles by Differential Filtration with Nanopore Filters
by
Antonio Peramo 1,* and Jose A. Diaz 2
Antonio Peramo 1,* and Jose A. Diaz 2
1
Department of Oral and Maxillofacial Surgery, 1150 W Medical Drive, MSRBII A560, University of Michigan, Ann Arbor, MI 48109, USA
2
Department of Surgery, Section of Vascular Surgery, University of Michigan, Ann Arbor, MI 48109, USA
*
Author to whom correspondence should be addressed.
Membranes 2012, 2(1), 1-15; https://doi.org/10.3390/membranes2010001
Received: 28 October 2011 / Revised: 16 December 2011 / Accepted: 22 December 2011 / Published: 27 December 2011
With the objective of making advancements in the area of pro-thrombotic microparticle characterization in cardiovascular biology, we present a novel method to separate blood circulating microparticles using a membrane-based, nanopore filtration system. In this qualitative study, electron microscopy observations of these pro-thrombotic mouse microparticles, as well as mouse platelets and leukocytes obtained using a mouse inferior vena cava ligation model of deep-vein thrombosis are presented. In particular, we present mouse microparticle morphology and microstructure using SEM and TEM indicating that they appear to be mostly spherical with diameters in the 100 to 350 nm range. The nanopore filtration technique presented is focused on the development of novel methodologies to isolate and characterize blood circulating microparticles that can be used in conjunction with other methodologies. We believe that determination of microparticle size and structure is a critical step for the development of reliable assays with clinical or research application in thrombosis and it will contribute to the field of nanomedicine in thrombosis.
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MDPI and ACS Style
Peramo, A.; Diaz, J.A. Physical Characterization of Mouse Deep Vein Thrombosis Derived Microparticles by Differential Filtration with Nanopore Filters. Membranes 2012, 2, 1-15.
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