The Conventional and Breakthrough Tool for the Study of L-Glutamate Transporters

In our recent report, we clarified the direct interaction between the excitatory amino acid transporter (EAAT) 1/2 and polyunsaturated fatty acids (PUFAs) by applying electrophysiological and molecular biological techniques to Xenopus oocytes. Xenopus oocytes have a long history of use in the scientific field, but they are still attractive experimental systems for neuropharmacological studies. We will therefore summarize the pharmacological significance, advantages (especially in the study of EAAT2), and experimental techniques that can be applied to Xenopus oocytes; our new findings concerning L-glutamate (L-Glu) transporters and PUFAs; and the significant outcomes of our data. The data obtained from electrophysiological and molecular biological studies of Xenopus oocytes have provided us with further important questions, such as whether or not some PUFAs can modulate EAATs as allosteric modulators and to what extent docosahexaenoic acid (DHA) affects neurotransmission and thereby affects brain functions. Xenopus oocytes have great advantages in the studies about the interactions between molecules and functional proteins, especially in the case when the expression levels of the proteins are small in cell culture systems without transfections. These are also proper to study the mechanisms underlying the interactions. Based on the data collected in Xenopus oocyte experiments, we can proceed to the next step, i.e., the physiological roles of the compounds and their significances. In the case of EAAT2, the effects on the neurotransmission should be examined by electrophysiological approach using acute brain slices. For new drug development, pharmacokinetics pharmacodynamics (PKPD) data and blood brain barrier (BBB) penetration data are also necessary. In order not to miss the promising candidate compounds at the primary stages of drug development, we should reconsider using Xenopus oocytes in the early phase of drug development.


Basic Background on EAAT2 and Other L-Glutamate Transporters in the Central Nervous System
Human excitatory amino acid transporters (EAATs) have five subtypes, EAAT1, 2, 3, 4, and 5 (EAAT1-3 [1], EAAT3 [2], EAAT4 [3], EAAT5 [4]).EAAT2 is the predominant excitatory neurotransmitter transporter that accounts for approximately 90% of L-glutamate (L-Glu) uptake in the forebrain [5], meaning that EAAT2 is essential for healthy brain functioning.In addition to refining synaptic transmission, one of the important roles of EAAT2 is protecting neurons from excitotoxicity, the neuron-specific cell death mechanism caused by prolonged elevation of extracellular L-Glu [6].EAAT2 is expressed mainly in astrocytes, but 5 to 10% of the total EAAT2 expression is found in neurons [7].The extracellular and intracellular electrochemical gradient of Na + is a driving force of the transport.The principle of EAAT2 transport is as follows: 1 glutamate transport is coupled to the influx of 3 Na + and 1 H + followed by the outflux of 1 K + , resulting in electrogenicity [8] (Figure 1A(a2)).This coupling maintains a transmembrane L-Glu concentration gradient ([Glu] in /[Glu] out ) exceeding 10 6 -fold under physiological conditions [8], i.e., the [Glu] in of astrocytes is approximately 2-3 mM L-Glu in their cytoplasm, and [Glu] out under restingstate conditions is approximately 25 nM [9].Transport against the concentration gradient uses ATP derived from the Na + /K + -ATPase pathway [10].
EAAT2 is also related to neuronal energy metabolism [11,12].When the intracellular Na + concentration is changed in astrocytes, lactate is released from astrocytes to the neuron as the energy source.Furthermore, EAAT2-induced intracellular Na + elevation drives the release of γ-aminobutyric acid (GABA) through the reversal of GABA transporter GAT-3, thereby resulting in tonic inhibition of neurons [13].This mechanism provides an adjustable, in situ negative feedback on the excitability of neurons in physiological conditions Membranes 2024, 14, x FOR PEER REVIEW 2 of 16 [8] (Figure 1A(a2)).This coupling maintains a transmembrane L-Glu concentration gradient ([Glu]in/[Glu]out) exceeding 10 6 -fold under physiological conditions [8], i.e., the [Glu]in of astrocytes is approximately 2-3 mM L-Glu in their cytoplasm, and [Glu]out under resting-state conditions is approximately 25 nM [9].Transport against the concentration gradient uses ATP derived from the Na + /K + -ATPase pathway [10].EAAT2 is also related to neuronal energy metabolism [11,12].When the intracellular Na + concentration is changed in astrocytes, lactate is released from astrocytes to the neuron as the energy source.Furthermore, EAAT2-induced intracellular Na + elevation drives the release of γ-aminobutyric acid (GABA) through the reversal of GABA transporter GAT-3, thereby resulting in tonic inhibition of neurons [13].This mechanism provides an adjustable, in situ negative feedback on the excitability of neurons in physiological conditions.C in ND96 solution containing 96 mM NaCl, 2 mM KCl, 1.8 mM CaCl 2 , 1 mM MgCl 2 , and 5 mM HEPES (pH 7.5) supplemented with 0.01% gentamycin.TEVC recordings from the oocytes were performed at room temperature (25 • C) using glass microelectrodes filled with 3 M KCl (resistance = 1-4 MΩ) and an Ag/AgCl pellet electrode.(a2).Substrate-and the coupling ion-transports by EAATs.Substrate, such as L-Glu, L-Asp, or D-Asp, transport through EAATs is coupled to the co-transport of 3 Na + and 1 H + followed by the counter transport of 1 K + .In addition, the binding of substrates and Na + to EAATs activates uncoupled Cl − anion currents.(B) (b1).A representative trace of L-Glu (50 µM for 2 min, black bar)-induced current obtained from Xenopus oocytes overexpressing EAAT2 clamped at −50 mV.(b2).IV relationship for L-Glu (50 µM)-induced EAAT2 current.To examine the IV relationship, the L-Glu-induced current was calculated through the subtraction of the steady-state current from the L-Glu-induced current.The curves were obtained with a holding potential of −60 mV applying an 8000 ms ramp pulse from −110 to +60 mV.Data are shown as the values normalized to that obtained with 50 µM L-Glu at −100 mV.Means, n = 5. (C) (c1).Model curves of transport-induced currents.The total L-Glu-induced EAAT currents (solid line: I total ), electrophysiologically recorded using TEVC methods from EAAT-expressing Xenopus oocytes, represent the sum of the coupled L-Glu transport currents (dotted line: I AA ) and the uncoupled Cl − anion currents (dotted line: I Cl ).(c2).The predicted reversal potential of the net current (I total ) is independent of substrate concentration when the concentration dependence of I AA and I Cl is the same.However, the amplitude of I total is dependent on the substrate concentration.(c3).The absolute reversal potential of I total is dependent on I AA relative to I Cl [14] (copyright permission has been obtained).(D) (d1).D-Asp uptake and charge translocation were simultaneously measured during a 100 s application of 100 µM [ 3 H] D-Asp to voltage-clamped oocytes expressing EAAT1 under 104 mM Cl − conditions.(d2).Voltage dependence of RI-labelled D-Asp flux and superimposed exponential (e-fold/75 mV) derived from fit of transport current under nominal Cl − -free conditions [14] (copyright permission has been obtained).(E) Overall structure of human EAAT2 as viewed from the membrane plane (left) and the intracellular side (right).The trimerization domain is blue and the transport domain is red.The protomer of EAAT2 is divided into two distinct functional components: one is a rigid scaffold domain that mediates interprotomer interactions and is located in the center of the trimer, and the other is a transport domain containing the substrate-binding site [15].(F) Schematic representation of the transport cycle of EAATs (elevator motion).The transport domain (red) moves across the membrane relative to the trimerization domain (blue).The transported L-Glu is pink.When L-Glu binds to the transport domain of the EAAT in outward facing state (top), the conformation changes to occluded state (middle) first, then changes to inward facing state (bottom), thereby releasing L-Glu into the cell.

Reasons to Choose Xenopus Oocytes for the Study of EAAT2
Cells used for the pharmacological and physiological study of EAAT2 should meet the following two requirements: the cells should express enough EAAT2, and the molecular functions of the cells can be quantitatively investigated.Xenopus oocytes meet these two requirements and are appropriate for the study of EAAT2.We will explain this in the following paragraphs.

Advantages of Xenopus Oocytes That Overexpress EAAT2
EAAT1 and EAAT2 are mainly expressed in astrocytes [43,44], while EAAT2 is also expressed in neurons, but to a lesser extent.However, in the primary culture of astrocytes, the expression level of EAAT2 is very low [16,45], and the functional predominance switches from EAAT2 to EAAT1 [46][47][48][49].In vitro substrate uptake assays are therefore performed using cell lines such as the Human Embryonic Kidney 293 (HEK 293) [50,51] and African green monkey kidney fibroblast-like (COS) cell lines [1,33], in which heterologous expression of cloned transporters has been induced.The difficulties of these in vitro assays are unignorable variations in the transfection ratios and the expression levels.In contrast, in terms of transfection, we inject the most probable amount of cRNA directly into Xenopus oocytes.Even though the expression levels depend on the target proteins, statistical analyses among experiments are possible [52].The following are the additional technical advantages of the use of Xenopus oocytes: ■ Direct injection enables to express the targeted proteins with a high success rate.

■
Approximately 200 or more oocytes at defolliculated stage V or VI that are suitable for cRNA injection can be collected from one Xenopus.Four to six operations with 1-week intervals are possible per one Xenopus.Additionally, three injections are possible for the oocytes collected in one operation.Therefore, ready-to-use oocytes can be obtained 12 times a month.

■
The transfection protocol is easy and feasible (Figure 1A(a1)).To overexpress the targeted protein in Xenopus oocytes, 50 nL of capped cRNA (10 ng) solution is directly injected into an oocyte by a nanoinjector installed with a glass microelectrode.An oocyte is a spherical cell with a diameter of 1-1.2 mm, large enough to confirm a successful injection by checking the swelling of the oocyte.Furthermore, the diameter of the microelectrode tip is 20-25 µm, which enables the minimization of membrane damage and the initiation of electrophysiological recording the next day.
■ By modulating the interval between the injection and the analysis, the expression level of the targeted proteins can be regulated.Conversely, experiments using oocytes expressing almost the same level of the targeted proteins can be performed.

■
The interactions of the targeted protein with the accessory subunits and/or modulatory proteins can be examined through the co-transfection of these proteins at the optimal ratio [e.g., protein interacting with C kinase 1 (PICK1), containing the postsynaptic density protein (PDZ) domain, with GLT1 (EAAT2 in rodents) [53]].

Measurement of the Activity of EAAT2 Expressed in Xenopus Oocytes
The quantification of the amount of radioisotope (RI)-labelled substrates transported into the oocytes is a conventionally and widely used protocol for the quantification of EAAT2 activity [24,54].In the case of EAAT2, however, because the transport activity depends on the membrane potential, accurate quantification can be performed under the membrane potential clamp (Figure 1D) [14].As described in Chapter 1, substrate transport is coupled to the influx of 3 Na + and 1 H + followed by the outflux of 1 K + , resulting in L-Glu transport-coupled currents (I AA ) [8].Furthermore, the uncoupled Cl − anion channel is opened (I Cl ) by substrate binding (Figure 1A(a2)).The model by Wadiche shows that the total L-Glu-induced EAAT currents (I total ) are the sum of I AA and I Cl [14] (Figure 1C(c1)).Electrophysiological techniques allow real-time detection of the effects of compounds on L-Glu transport (Figure 1B(b1)).Furthermore, these techniques can also detect heteroexchange associated with transport, which is impossible to detect by RI-labelled substrate assay.The current-voltage (IV) relationships of I AA show inward rectification with no reversal potential, that is the membrane potential at which the direction of ionic current reverses, while those of I Cl show linear with reversal potential and outward current at positive membrane potentials.In I total , the outward current is due to I Cl and the reversal potential is dependent on the relative magnitude of I AA and I Cl (Figure 1C(c3)).The amplitude of I total is dependent on the amount of transported substrates, while the reversal potential of I total is independent of the amount of substrates transported (Figure 1C(c2)).
EAAT1-5 differs in the proportion of I AA and I Cl in I total .The neuronal transporters EAAT4 and EAAT5 act primarily as Cl − anion channels due to I Cl dominance [3,4].On the other hand, EAAT1, EAAT2, and EAAT3 have smaller I Cl than I AA .Specifically, I AA is predominant, and the contribution of the Cl − anion current is very small in EAAT2 I total [14].In line with this, the open probability of Cl − anion channels of EAAT2 transfected in HEK293 cells was confirmed to be 0.06 ± 0.01% [55].We have confirmed that IV relationships for the L-Glu-induced EAAT2 current have no reversal potential up to +60 mV and are very similar to I AA in Xenopus oocytes overexpressing EAAT2 by two-electrode whole-cell voltage clamp (TEVC) methods (Figure 1B(b2)).This result indicates that EAAT2 transport activity can be quantified by substrate-induced EAAT2 currents.In support of this, in the simultaneous recordings of the EAAT1 current and RI-labelled D-Asp uptake under Cl − free conditions, EAAT1 current showed clear voltage dependence similar to D-Asp flux (Figure 1D) [14].
The following are the additional advantages of electrophysiological recording in Xenopus oocytes.

■
Measuring EAAT2 currents using electrophysiological techniques enable to detect the acute effects immediately (Figure 1B(b1) and Figure 2B,C), while the radioactivity detection of RI-labelled substrates has a time lag between the experiment and detection.

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Because a Xenopus oocyte is a large single cell, TEVC methods can be applied.Among the various types of electrophysiological techniques, it is rather easy to become proficient in performing TEVC.Furthermore, the oocyte membrane is so strong and stable that obtaining recordings from 10-30 oocytes per day and for longer than 30 min per oocyte is possible [53,56,57].

■
In addition to the whole oocyte clamp, more detailed experiments, such as single channel recording, outside-out patch clamp, and inside-out patch clamp, can be performed [59,60].

■
It is possible to modify intracellular conditions in addition to the extracellular condition during recording.For example, it is possible to change intracellular conditions by filling glass microelectrodes with compounds such as H 2 O 2 or DTT [57].The extracellular conditions can be modulated by changing the pH, Cl − concentration, etc.Furthermore, the chronic effects of compounds can be examined [61] through incubation with the conditioned medium for ~2 days.

Application of Molecular Biological Techniques to Xenopus Oocytes
To investigate the structural and functional properties of the targeted functional proteins in detail, site-directed mutagenesis, i.e., cysteine substitution, chimaera, etc., can be applied.The substituted cysteine accessibility method (SCAM) is typically used to investigate the structural information of membrane proteins [62,63].In SCAM, local steric and electrostatic environments are probed by sulfhydryl reactive reagents because of the reactiveness of the substituted cysteines.This method has been applied to clarify the modulator binding sites and the regions related to the different functional states of many kinds of membrane proteins.The membrane topologies of EAATs have been studied by this method as well [64,65].In addition, by inserting some pairs of cysteines and assaying their ability to form disulfide bonds, the proximity and mobility relationships between specific positions within the transporters have been clarified [66][67][68].In Greek mythology, a chimaera is a monster that is a fusion of a lion, goat, and dragon.In molecular biology, a chimaera refers to cells or animals in which two or more genotypes are fused.In terms of the study of EAATs, quite a few chimaera combinations have been made to identify the binding sites for substrates and ions [69,70].This technique was used to define the functional domains related to transport blockers.Vandenberg's group defined the combination of helical hairpin (HP) 1, HP2, transmembrane (TM) 7, and TM8 to determine the sensitivity to transport blockers such as kainic acid [69] and 3-methylglutamate [70] (see Figure 2D about the membrane topology of EAATs).DHA is docked to the lipid crevice (carbon: purple spheres; hydrogen: white spheres).(I) Docking poses of DHA in the lipid pocket in the vicinity of HP2 according to the induced fit docking protocol.The scaffold domain and transport domain are shown in green and grey ribbons, respectively.The carbons in DHA and EAAT2 L434 are represented by purple and yellow sticks, respectively.The atoms in L-Glu are shown as follows: carbon, blue sphere; hydrogen, white sphere; oxygen, red sphere; nitrogen, hidden.Na + is shown as a pink sphere.Two types of DHA conformations could be visualized according to the position of the carboxylic group, i.e., one with a carboxyl group on the upper side (i1) and the other with a carboxyl group on the lower side (i2).Both of them have similar U-shaped conformations.The inset shows the DHA conformations in each case.The three-dimensional position of DHA is in close proximity to the L-Glu binding site and Na + binding site.Error bars represent the mean ±SD.The numbers written within parentheses in the respective figures represent the number of independent experiments.Statistical differences between groups were determined by two-tailed paired Student's t test.p values are indicated in each figure panel [58].

New Findings about the Interactions between PUFAs and EAAT2 Obtained with Xenopus Oocyte Experiments
In this section, we will present our recent work regarding the modulation of EAAT2 function by docosahexaenoic acid (DHA) [58], in which electrophysiological and molecular biological techniques were applied to Xenopus oocytes overexpressing EAAT2.Because EAAT1 (GLAST in rodents) is also expressed in astrocytes in addition to EAAT2 (especially in the cerebellum) [71], we examined the effects of DHA (C22:6) (Figure 2A) and other polyunsaturated fatty acids (PUFAs) on EAAT2 and compared those on EAAT1.DHA has long been known to enhance synaptic transmission through multiple mechanisms, and L-Glu transporters are candidate proteins involved in target molecules.
We found that DHA significantly increased L-Glu-induced EAAT2 currents (Figure 2B) but slightly decreased L-Glu-induced EAAT1 currents (Figure 2C) using TEVC in Xenopus oocytes transfected with EAAT1 or EAAT2.Although there are some reports indicating the effects of DHA on EAATs, whether DHA interacts with EAATs directly has yet to be elucidated.To solve this problem, we attempted to identify the key amino acid for the DHA-EAAT2 interaction by EAAT1/2 chimaeras, focusing on the differences in the effects of DHA on EAAT1 and EAAT2.EAAT2 functions as a homotrimer, with each protomer having eight TM sites (TM1-8) and two HPs (HP1 and HP2), which are re-entrant loops (Figure 2D).The protomer is divided into two distinct functional components: one is a rigid scaffold domain (TM1, 2, 4, and 5) that mediates interprotomer interactions and is located in the center of the trimer, and the other is a transport domain (TM3, 6, 7, 8, HP1, and HP2) containing the substrate-binding site [15,72,73] (Figures 1E and 2H).When we examined the effect of DHA-coenzyme A (DHA-CoA), a membrane-impermeable DHA analogue (Figure 2A), DHA-CoA increased the EAAT2 current almost to the same extent as DHA, suggesting that DHA approaches EAAT2 from the outside of cells.Therefore, we focused on the TM7b-HP2a sequence, the extracellular region of the transport domain (red square in Figure 2D(d1)).We first constructed EAAT2-based EAAT1/2 chimaeras.However, as sometimes occurs in chimaera experiments, the EAAT2-based chimaera for which its TM7b-HP2a was substituted to that of EAAT1 was nonfunctional (EAAT2 [EAAT1 TM7B-HP2a]) (Figure 2D(d2)).We therefore changed the chimaera from the EAAT2-based chimaera to the EAAT1-based chimaera.When the EAAT1 TM7b-HP2a region was replaced by that of EAAT2 (EAAT1 [EAAT2 TM7b-HP2a]) (Figure 2D(d3)), the effect of DHA on the L-Glu current was enhanced.We first suspected that the least conserved region in TM7b-HP2a, the "connector" in Figure 2D(d4),E, could cause the differences in responses between EAAT1 and EAAT2.When we replaced the "connecter" sequence of EAAT1 with that of EAAT2, i.e., in EAAT1 (EAAT2 connecter), the augmentative effect of DHA was not induced.We therefore focused on the amino acids in TM7b-HP2 other than the "connector".Six amino acids (Val407, Met415, Val426, Val428, Leu430, and Leu434 in EAAT2) in TM7b-HP2a are different between EAAT1 and EAAT2 (black arrowheads in Figure 2E), so we back-mutated the above six amino acids one by one to the original EAAT1 amino acid.Among the mutants, only L434A showed a complete disappearance of the effect of DHA.We confirmed that a single mutation of Leu434 in WT EAAT2 to Ala completely suppressed the effect of DHA (Figure 2F).Furthermore, a single mutation of Ala435 in WT EAAT1 (corresponding to EAAT2 Leu434) to Leu also changed the effect from inhibition to enhancement (Figure 2G).The data above strongly suggest the direct interaction of DHA with EAAT2.
To confirm the feasibility of our hypothesis, we next performed a docking simulation between DHA and EAAT2.The dynamics of the scaffold domain and transport domain are involved in the substrate transport processes of L-Glu transporters.The first transport concept was developed by Jardetzky as an alternating access model in 1966 [74]: outward facing state (OFS), occluded state, and inward facing state (IFS).The structural basis for the transporter function is the elevator movement (Figure 1F) of the scaffold domain/transport domain backed up by the four crystal structures of Glt Ph [OFS: [72,75] → intermediate (i) OFS: [76] → unlocked IFS: [77] → IFS: [78]].We performed a docking analysis of DHA and EAAT2 OFS using EAAT1 as a template (protein data bank ID [PDBID]: 5LLM) [79].Among seven candidate combinations of docking sites and DHA poses, which were proposed by a standard-induced fit docking protocol [80][81][82], the two top-scoring combinations are shown in Figure 2H,I.By performing calculations with SiteMap (SiteMap, Schrödinger, LLC, New York, USA), we found that DHA was docked in the lipid crevice, facing the interface of the transport domain/scaffold domain, which is close to the binding sites of the substrate and Na + .The DHA poses are U-shaped in both cases (Figure 2I) [58].

Some PUFAs Modulate EAATs as Allosteric Modulators
Docking analysis of EAAT2 and DHA suggests that the most stable docking site is located at the transport domain/scaffold domain interface close to the binding sites of the substrates and Na + .Some allosteric modulators, such as GTG949, an EAAT2 selective enhancer [40], and UCPH101, an EAAT1 selective blocker [79,83], interact with the transport domain/scaffold domain interface and affect the movement of the transport domain, namely, elevator motion [79].Detailed structural studies on Glt Ph , an archaeal EAAT homologue of the thermophilic prokaryote Pyrococcus horikoshii, provide supportive evidence for the direct interactions between transporters and lipids, including PUFAs [75,77,84].In addition, the crystal structure analysis of Glt Ph in the unlocked IFS suggests that the binding of lipids to the interface may facilitate the sliding of the transport domain.Most recently, cryo-electron microscopy (cryoEM) analysis has provided evidence for the direct interactions of EAAT2 and lipids [73].Our data and recent related findings suggest that DHA allosterically regulates EAAT2 by enhancing the sliding of the transport domain.Furthermore, we also clarified that among 10 fatty acids [docosapentaenoic acid (DPA, C22:5), docosatetraenoic acid (C22:4), docosatrienoic acid (C22:3), eicosapentaenoic acid (EPA, C20:5), arachidonic acid, (ARA, C20:4), eicosatrienoic acid (C20:3), eicosadienoic acid (C20:2), α-linolenic acid (ALA, C18:3), linoleic acid (C18:2), and oleic acid (C18:1)], only DPA, EPA, ARA, and ALA showed augmentative effects on EAAT2 and slight inhibition of EAAT1 through the same mechanisms that require interactions with Leu434.Our data further suggest that a particular three-dimensional structure of PUFAs leads to the allosteric modulation of EAAT2.

Physiological Significance: The Potential of DHA as a Neurotransmission Modulator
The interactions between PUFAs and synaptic transmission were previously reported for ARA (ω-6, C20:4) [85][86][87].However, there remain many PUFAs whose effects on synaptic transmission are unknown.DHA is the most abundant ω-3 PUFA in the mammalian brain [88][89][90] and the major constituent of membrane phospholipids [91].DHA is synthesized in astrocytes from precursor fatty acids [92][93][94] and supplied to neuronal membranes as well.Under stable conditions, DHA is esterified to phosphatidylserine or phosphatidylethanolamine.When L-Glu stimulates neurons and astrocytes via L-Glu receptors [95,96], Ca 2+ -independent phospholipase A2 (iPLA2) is activated, thereby triggering DHA release [97,98].The previous data and our findings raise the possibility that endogenous DHA modulates excitatory synaptic transmission.Some studies have reported favorable roles for DHA in human cognition and behavior; however, consistent conclusions have not been obtained [99].In animal models and in vitro models, supporting data have been shown.EAATs control synaptic transmission, neurotransmitter spillover, and long-term plasticity [100][101][102][103]. Furthermore, DHA facilitates corticostriatal long-term potentiation (LTP) [104], the basic mechanisms for learning and memory.In support of this, selective inhibition of iPLA2 abolishes the expression of LTP [105], and DHA recovered the abolishment of LTP.

Summary
We could clarify the direct interaction between EAAT1/2 and DHA by applying electrophysiological and molecular biological techniques to Xenopus oocytes.Furthermore, we could make the difference between EAAT1 and EAAT2 clear.Our results indicate that Xenopus oocytes have great advantages in the studies about the interactions between molecules and EAATs, especially in the case when the expression levels are small in cell culture systems without transfections.Xenopus oocytes are also proper to study the mechanisms underlying the interactions, i.e., the docking sites, allosteric modulation, etc.Based on these data, we can proceed the study to the next step.The physiological role and significance can be investigated.In the case of EAAT2, the effects on the neurotransmission should be examined by electrophysiological approach using acute brain slices, etc.For new drug development, PKPD data and BBB penetration should also be needed.In order not to miss promising candidate compounds, we should reconsider using Xenopus oocytes in the early phase of drug development.
Author Contributions: K.S. designed this work and wrote the paper.K.T. performed the experiments and wrote the paper.All authors have approved the present version of the manuscript and have agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.All authors have read and agreed to the published version of the manuscript.

Figure 2 .
Figure 2. (A) The chemical structures of DHA and DHA-CoA.(B) Representative traces of L-glutamate (L-Glu, 50 µM for 2 min, black horizontal line)-induced current obtained from Xenopus oocytes overexpressing EAAT2 clamped at −50 mV in the absence or presence of DHA (100 µM for 2 min, grey horizontal line).When coapplied, DHA increased the L-Glu-induced EAAT2 current, and the effect disappeared after washout.(C) Representative traces of L-Glu-induced EAAT1 currents in the absence or presence of DHA.When the compounds were coapplied, DHA tended to decrease the EAAT1 current, and the effect disappeared after washout.(D) Topology of EAAT1, EAAT2 (d1).EAAT1/2 is organized into eight transmembrane (TM1-8) and two helical hairpins (HP1 and HP2), which are re-entrant loops.TM7b-HP2a sequence and connector sequence in the red square.d2-d4.EAAT1-EAAT2 hybrid chimeras: EAAT2 (EAAT1 TM7b-HP2a) (d2), EAAT1 (EAAT2 TM7b-HP2a) (d3), and EAAT1 (EAAT2 connector) (d4).(E) Amino acid alignment from TM7b to HP2a of EAAT2 and EAAT1.The common amino acids are shown on a black background.Single amino acid backmutations were performed at the sites indicated by black arrowheads in the EAAT1 (EAAT2 TM7b-HP2a) chimaera.(F) Left, topology of EAAT2 L434A.Right, comparison of the effects of DHA on EAAT2 and EAAT2 L434A.Data are shown as rates of increase by DHA.(G) Left, topology of EAAT1 A435L.Right, comparison of the effects of DHA on EAAT1 and EAAT1 A435L.Data are shown as rates of increase by DHA.(H) Proposed binding conformation for DHA in the transport domain/scaffold domain interface of the EAAT2 homology model in the outward facing state (OFS).(h1).Extracellular view of the trimerized EAAT2 OFS homology model based on the EAAT1 crystal

Funding:
This work was partly supported by a Grant-in-Aid for Young Scientists from the Ministry of Education, Science, Sports, and Culture, Japan (KAKENHI 18700373, 21700422, 17K08330), a Grant for the Program for Promotion of Fundamental Studies in Health Sciences of NIBIO, Japan, a grant for Research on Risks of Chemicals, a Labor Science Research Grant for Research on New Drug Development from the MHLW, Japan, a Research Grant on Regulatory Harmonization and Evaluation of Pharmaceuticals, Medical Devices, Regenerative and Cellular Therapy Products, Gene Therapy Products, and Cosmetics from AMED, Japan (iPSC Non-Clinical Experiments for Nervous System Project: iNCENS), and a Grant-in-Aid from Hoansha Foundation awarded to K.S. Institutional Review Board Statement: Not applicable.

Table 1 .
Pharmacological profiles of EAAT2 inhibitors and enhancers.The values in the selectivity column represent Kd for substrates, Ki for blockers, and EC50 for enhancers for the respective EAAT subtypes.The arrows in "effect on EAAT2 current" column represent whether the effect is inhibition (downward) or enhancement (upward).-means no effects.