A Functionalized Membrane Layer as Part of a Dressing to Aid Wound Healing

Purpose: This study is an approach to a dressing platform based on support functionalized with oxygenating factors within an alginate layer, constituting a safe and even contact surface for interface with a wound. Methods: An alginate layer with incorporated oxygenating elements deposited on the support patch was assessed. As an oxygenating factor, perfluorooctyl was applied, and the layer coatings in two options, cross-linked and not, were evaluated. The function of human dermal fibroblast cells cultured in the presence of these constructs was analyzed, as well as their morphology using flow cytometry, fluorescence microscopy, and scanning electron microscopy. In addition, the membrane coating material was assessed using FTIR, AFM, and SEM-EDX characterization. Results: The applied membrane coatings adsorbed on the patch ensured the viability of the human fibroblasts cultured on the membranes during 10 days of culture. However, on the sixth day of culture, the percentage of live cells grown in the presence of cross-linked alginate with oxygenating factor ((ALG-PFC)net) was significantly higher than that of the cells cultured in the presence of the alginate coatings alone. SEM-EDX analysis of the (ALG-PFC)net confirmed the presence of oxygenating and cross-linking factors. In addition, the regular granular branched structure of the layer coating material involving the oxygenating and cross-linking factors was observed using the AFM technique. Conclusion: The topography of the layer coating material involving the oxygenating and cross-linking factors ensures an even contact surface for interface with the wound. Considering 5-day intervals between dressing replacements, the platform with an oxygenating configuration ensuring the growth and morphology of the human fibroblasts can be recommended at this time as an element of a dressing system.


Introduction
Wound treatment is a significant problem in modern societies. It is estimated that only in Europe, around 1.5-2 million people suffer from acute and chronic wounds [1]. The situation is aggravated by the fact that the success of applied therapy largely depends on the overall patient's condition. For example, coexisting diseases worsen wound healing, affecting the effectiveness of treatment. Obesity, diabetes, and other illnesses of civilization have a negative impact, especially on the ageing population of developed countries, where these comorbidities are common. As a result, the clinical and economic burden of treating containing fluorinated photosensitizer is another potential application of PFC, which can also act as a 19 F magnetic resonance imaging agent. PFC-based systems for diagnosis and treatment have been constantly developed [23,24]. Furthermore, perfluorinated compounds, widely used in consumer and industrial products and processes, belong to a class of compounds that tend to persist in the environment. Perfluorinated compounds can be detected, e.g., in different foodstuffs, water, plants, mammals, and humans. Therefore, the toxic effects and the contribution of individual pathways of PFC contamination of humans (such as diet, food contact materials, non-food personal items, and indoor and outdoor air) to total contamination have been investigated [25,26]. Nevertheless, because of the strength of the carbon/fluorine bond, the PFC molecules considering their chemical stability and resistance to biological degradation find biomedical applications.
Considering the global increase in wound management's clinical and economic burden, new generations of products functionalized to obtain the properties that can affect the wound-healing process have been developed. Our goal was to build a platform that would meet this demand, built of commercially available dressing support functionalized with highly disposable substrates.
The study aims to approach a dressing platform based on support functionalized with oxygenating factors. The function of cells cultured in the presence of these constructs was analyzed, as well as their morphology using flow cytometry, fluorescence microscopy, atomic force microscopy, and scanning electron microscopy.
The alginate, which has a natural ability to create stable gels in reaction with solutions containing divalent cations, was applied as a base material. Its gelling is the effect of a conglomeration of the segments of polyguluronic acid in clusters where calcium ions are scattered in the space between the chains ('egg-box model') [27].
The platform with an oxygenating factor within cross-linked layer coating in a configuration with a commercially available patch as support has not been reported before for wound dressing applications.

Preparation of Polyelectrolyte Membranes Deposited on the Support AQUACEL Foam
The following solutions were used to modify the patch: 2.2% sodium alginate in PBS; 2.5% calcium chloride in physiological saline; mixture of 1-Bromoheptadecafluorooctane (liquid concentration from the brand bottle) and alginate (2.2%) in a 1:9 volume ratio (ALG-PFC).

Preparation of Coatings
A circular patch with an area of around 1.5 cm 2 (cut from the brand patch) was covered with the following coatings:

Cell Culture
Human Dermal Fibroblast (HDF) cells were maintained under standard culture conditions (5% CO 2 , 37 • C) in Fibroblast Growth Medium. Cells were grown to approximately 80% confluency. The medium was then removed from the culture bottles, whereas the cells were washed with PBS without calcium and magnesium ions and trypsinized. Next, 1 mL of a suspension of HDF cells at a concentration of 5 × 10 3 was added to the culture wells. After one day, the patch was placed in the well with the modified surface facing down to obtain direct contact with fibroblasts. After 3-, 6-, and 10 days of culture, the supports were removed, the cells in the culture wells were trypsinized, and then, the viability of the cells was examined using propidium iodide in a flow cytometer. As a control, the cells were grown in the presence of unmodified supports.
Additionally, the surface of the glass slices was modified with the coatings. Then, prepared slices were placed in the wells of the culture plate. Next, the HDF cell suspension was added. Cells were grown for 10 days. After 3-, 6-, and 10-day cultivation, the cells were examined using fluorescence microscopy.

Flow Cytometry
Flow cytometry was applied to examine the cells' functioning, wherein data acquisition was performed on the Canto II flow cytometer (Becton Dickinson Immunocytochemistry Systems, Franklin Lake, NY, USA). Then, the results were processed by the FACS Diva software system (Becton Dickinson, Franklin Lake, NY, USA).

MTT Assay
The (3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess cellular mitochondrial activity was performed. Shortly, the cells were seeded on the membrane films. After 3-, 6-, and 10 days of culture, the MTT solution at the concentration of 5 g/L was added to the culture in a 1:10 dilution of the medium. Next, the cells were incubated for 2 h at 37 • C with 5% CO 2 . Afterward, the solution was discarded, and DMSO was added. After 15 min of shaking, absorbance was measured at 550 nm using a spectrophotometer (HP 8452 diode-array spectrophotometer).

Reactive Oxygen Species (ROS) Assessment in HDF Cells
The ROS level was determined using a DCFM-DA assay (Invitrogen). After 3-, 6-, and 10 days of culture, the cells were washed with PBS and, at a final concentration of 10 5 cells/mL, incubated with DCFM-DA for 30 min at 37 • C, protected from light. As a positive control, the cells labeled with DCFMDA were incubated with 50 µM tertbutyl hydrogen peroxide (TBHP) for 4 h. TBHP mimics ROS activity to oxidize DCFDA to fluorescent DCF. Then, the ROS accumulation in cells was assessed using flow cytometry.

Bacterial Strain and Growth Conditions
Bacterial cells were cultivated in LB broth (Biomaxima, Lublin, Poland) at 37 • C with shaking at 120 rpm. Overnight cultures were diluted in fresh medium to an optical density (OD) of 0.05 and transferred to a 24-well plate (0.5 mL per well).

The Material Influence on Bacterial Proliferation Examination
The material influence on selected bacterial strain proliferation was assessed. The E. coli bacterial strain was cultivated in the presence of the evaluated membranes for 24 h. The proliferation of bacterial cells was assessed spectrophotometrically by OD measurement at 600 nm after 24 h of cultivation. During these analyses, only bacteria present in the supernatant were evaluated.

AFM Evaluation
A Multimode 8 Nanoscope atomic force microscope (AFM, Bruker, Billerica, MA, U.S.A.) was used to image the samples' surfaces. Silicon cantilevers with a spring constant of ca. 5 Nm −1 (TapDLC-150, BudgetSensors, Sofia, Bulgaria) were applied for imaging in PeakForce TappingTM microscopy mode. The sample preparation procedure includes the application of 150 µL of solution on the clean mica plate, V1 grade (NanoAndMore GmbH, Wetzlar, Germany). After 10 min, the plates were rinsed with deionized water and dried under a gentle stream of argon.

Fourier Transform Infrared (FT-IR) Spectroscopy
The infrared spectra were recorded using the Nicolet iS50 FT-IR spectrometer (Thermo Scientific, Waltham, MA, USA) with a DTGS detector. An iTR attenuated total reflection accessory with diamond crystal was used for all experiments. All experiments were performed with a resolution of 4 cm −1 , and typically, 32 scans were taken for each sample. A 10 µL drop of solution was deposited in the crystal and left to dry under a gentle air stream.

SEM-EDX Evaluation
SEM-EDX characterization was carried out with a Crossbeam 540X (Carl Zeiss Microscopy GmbH, Jena, Germany) scanning electron microscope with an X-FEG cathode. In addition, the EDX maps were collected with the X-MAXN spectrometer (Oxford Instrument, Oxford, UK) operating at 15 keV. Samples were lyophilized before introducing to introduction to the microscope chamber.

Fluorescence Staining
For fluorescence staining, cells immobilized on the layers deposited on glass coverslips were fixed in 4% paraformaldehyde (PFA) in PBS at room temperature (20 • C) for 15 min. Then, cell membranes were permeabilized using TRITON X100 detergent to allow dyes to penetrate individual cells. Then, the fluorochrome-conjugated phalloidin staining F-actin was added. Phalloidin is a toxin isolated from the phylum Amanita fungus (Amanita phalloides), which binds directly to filamentous actin (F-actin) in fibroblasts. After that, the DAPI solution-fluorochrome, specifically staining DNA-was added to the cells to visualize single cells. Under UV light, cell nuclei stained DAPI shows blue fluorescence. After that, the sample was washed in PBS and analyzed in an IX70 fluorescence microscope (Olympus, Tokyo, Japan). The cytoskeleton's blue DAPI fluorescence (λ = 460-500 nm) of cell nuclei and red phalloidin fluorescence (λ = 570 nm) were assessed.

Statistical Analysis
The mean values and standard deviations and the significance of difference were calculated using Statistica 7.1 software. Values where p < 0.05 were assumed to be significant.

Results and Discussion
1-Bromoheptadecafluorooctane, PFC (also called perfluorooctyl bromide or heptadecafluorooctyl bromide) is one of the most broadly analyzed perfluorocarbons. According to Lumb, this 60% emulsion will bring about 50 mL of oxygen per 100 mL on equilibration with 100% oxygen (conditions: atmospheric pressure) [28]. Moreover, it is assumed that perfluorooctyl bromide can dissolve four times more oxygen than CO 2 [29]. Therefore, in our study, PFC was applied as an oxygenating agent to potentially improve the properties of the designed dressings. ALG-PFC coatings were prepared using the layerby-layer method. The experiments were also performed for cross-linked polyelectrolyte layers-(ALG-PFC) net .

Evaluation of the Functioning of Human Fibroblast Cells in the Presence of a Patch Modified with the Membrane Coating Layer
The developed membrane coatings adsorbed on the support patch were examined for cytotoxicity on the HDF human cell line in vitro. Cells were cultured on modified patches for 10 days. The cells cultured in the presence of patches without membrane coating served as a negative control during 10 days of culture. Cell function and morphology were evaluated quantitatively by flow cytometry and MTT and qualitatively using fluorescent microscopy.
To quantitatively assess the condition of cells cultured in the presence of the designed membranes, flow cytometry analysis was performed on the human dermal fibroblasts line (HDF) as a model. The obtained results showed that after 3 days of culture, no significant differences in viability were found between the cells grown in the presence of the patches with tested layers and the control. Furthermore, after 6 days of culture, no significant differences were found between the viability of cells cultured in the presence of ALG, ALG net , and ALG-PFC coatings; however, the values of ALG, ALG net , and ALG-PFC were significantly lower compared with control. In addition, the percentage of live cells grown in the presence of the (ALG-PFC) net was significantly higher than that of the cells cultured in the presence of the ALG and ALG net coatings. In addition, the ALG and ALG net mean percentage value was higher than the control. Moreover, after 10 days of culture, the values obtained for the cells grown in the presence of all coatings tested were higher compared with control. Finally, no significant differences were found between the viability of cells cultured in the presence of ALG, ALG net , ALG-PFC, and (ALG-PFC) net coatings. Therefore, considering 5-day intervals between dressing replacement, the cross-linked coating shell (ALG-PFC) net has been selected for further research. During the modification process with PFC, the hydrolyzable groups react with the hydroxyl groups present in alginate, forming a chemically bonded hydrophobic layer that may impact oxygen transport to the cells. The results of the cytometric test are shown in Figure 1.

with the Membrane Coating Layer
The developed membrane coatings adsorbed on the support patch were examined for cytotoxicity on the HDF human cell line in vitro. Cells were cultured on modified patches for 10 days. The cells cultured in the presence of patches without membrane coating served as a negative control during 10 days of culture. Cell function and morphology were evaluated quantitatively by flow cytometry and MTT and qualitatively using fluorescent microscopy.
To quantitatively assess the condition of cells cultured in the presence of the designed membranes, flow cytometry analysis was performed on the human dermal fibroblasts line (HDF) as a model. The obtained results showed that after 3 days of culture, no significant differences in viability were found between the cells grown in the presence of the patches with tested layers and the control. Furthermore, after 6 days of culture, no significant differences were found between the viability of cells cultured in the presence of ALG, ALGnet, and ALG-PFC coatings; however, the values of ALG, ALGnet, and ALG-PFC were significantly lower compared with control. In addition, the percentage of live cells grown in the presence of the (ALG-PFC)net was significantly higher than that of the cells cultured in the presence of the ALG and ALGnet coatings. In addition, the ALG and ALGnet mean percentage value was higher than the control. Moreover, after 10 days of culture, the values obtained for the cells grown in the presence of all coatings tested were higher compared with control. Finally, no significant differences were found between the viability of cells cultured in the presence of ALG, ALGnet, ALG-PFC, and (ALG-PFC)ne coatings. Therefore, considering 5-day intervals between dressing replacement, the crosslinked coating shell (ALG-PFC)net has been selected for further research. During the modification process with PFC, the hydrolyzable groups react with the hydroxyl groups present in alginate, forming a chemically bonded hydrophobic layer that may impact oxygen transport to the cells. The results of the cytometric test are shown in Figure 1.  To verify the mitochondrial activity of the cells cultured in the presence of the designed surfaces, we applied an MTT assay.
The presence of PFC within the ALG membrane (ALG-PFC) supports the function of HDF cells after 3 days of culture, achieving values comparable with the control. After 3-day culture on membrane ALG, ALGnet, (ALG-PFC) net meanly 40% of control value was observed, whereas, after 6 days of culture, the ALG membrane maintains HDF 'cells' func-tion comparable with the control. Moreover, the membrane ALG-PFC exhibit significantly higher mitochondrial activity than the control. The (ALG-PFC) net value was comparable with the value obtained on the third day of culture. On the contrary, the (ALG-PFC) net value was significantly lower than the 6-day value after 10 days of culture ( Figure 2). It is true to say that the application of the oxygenating and/or cross-linking factors did not negatively affect HDF cells concerning the viability; however, the fluctuation of mitochondrial activity during the culture can be observed for the support patches with coating involving oxygenating factor and/or cross-linking factor. It can be noted that ROS can affect the MTT results. Mitochondrial metabolism includes the buildup of potentially damaging levels of reactive oxygen species (ROS), Ca 2+ , etc., which must be normalized [30]. Response to stress caused by the introduction of oxygenating and/or cross-linking factors may cause both intra-and inter-mitochondrial redox-environment changes leading to ROS release. The fluctuations of values noticed in the MTT test may, to some extent, reflect it.
To verify the mitochondrial activity of the cells cultured in the presence of the designed surfaces, we applied an MTT assay.
The presence of PFC within the ALG membrane (ALG-PFC) supports the function of HDF cells after 3 days of culture, achieving values comparable with the control. After 3day culture on membrane ALG, ALGnet, (ALG-PFC)net meanly 40% of control value was observed, whereas, after 6 days of culture, the ALG membrane maintains HDF 'cells' function comparable with the control. Moreover, the membrane ALG-PFC exhibit significantly higher mitochondrial activity than the control. The (ALG-PFC)net value was comparable with the value obtained on the third day of culture. On the contrary, the (ALG-PFC)net value was significantly lower than the 6-day value after 10 days of culture ( Figure 2). It is true to say that the application of the oxygenating and/or cross-linking factors did not negatively affect HDF cells concerning the viability; however, the fluctuation of mitochondrial activity during the culture can be observed for the support patches with coating involving oxygenating factor and/or cross-linking factor. It can be noted that ROS can affect the MTT results. Mitochondrial metabolism includes the buildup of potentially damaging levels of reactive oxygen species (ROS), Ca 2+ , etc., which must be normalized [30]. Response to stress caused by the introduction of oxygenating and/or cross-linking factors may cause both intra-and inter-mitochondrial redoxenvironment changes leading to ROS release. The fluctuations of values noticed in the MTT test may, to some extent, reflect it. Evaluating the ROS accumulation in cells cultured in cross-linked layer coatings presence using flow cytometry, it was observed that after 3 days of HDF culture in the presence of (ALG)net and (ALG-PFC)net, the share of cells FITC positive was meanly 30% and 23%, respectively, compared with positive control. After 6 and 10 days, this value diminished for (ALG)net to about 3% and 0%, respectively, and for (ALG-PFC)net to 7% and 3%, respectively. The lowest value for (ALG-PFC)net on the sixth day seems to be consistent with the higher value of mitochondrial activity obtained for this coating at that time. (Figure 3). Evaluating the ROS accumulation in cells cultured in cross-linked layer coatings presence using flow cytometry, it was observed that after 3 days of HDF culture in the presence of (ALG) net and (ALG-PFC) net, the share of cells FITC positive was meanly 30% and 23%, respectively, compared with positive control. After 6 and 10 days, this value diminished for (ALG) net to about 3% and 0%, respectively, and for (ALG-PFC) net to 7% and 3%, respectively. The lowest value for (ALG-PFC) net on the sixth day seems to be consistent with the higher value of mitochondrial activity obtained for this coating at that time. (Figure 3).

Evaluation of the Functioning of Microorganisms Cultured in the Presence of the Membranes
The influence of the material on E. coli was assessed to analyze whether the (ALG-PFC)net induces the growth of the bacterial cells. Wherein the functioning of bacterial cells cultured for 24 h in the presence of selected membrane ((ALG-PFC)net and ((ALG)net) for comparison were investigated. The membrane layer material was added to the bacterial cells suspension in a volume ratio of 1:5. The negative control was bacterial cells maintained in the absence of the membranes. The optical density of the bacterial cell suspension at 600 nm (OD600) was employed to evaluate the function of bacterial cells by applying a TECAN reader, measuring 9 wells. The experiments were carried out in 6 replications. The results of the bacterial function test are presented below (Figure 4).

Evaluation of the Functioning of Microorganisms Cultured in the Presence of the Membranes
The influence of the material on E. coli was assessed to analyze whether the (ALG-PFC) net

Evaluation of the Functioning of Microorganisms Cultured in the Presence of the Membranes
The influence of the material on E. coli was assessed to analyze whether the (ALG-PFC)net induces the growth of the bacterial cells. Wherein the functioning of bacterial cells cultured for 24 h in the presence of selected membrane ((ALG-PFC)net and ((ALG)net) for comparison were investigated. The membrane layer material was added to the bacterial cells suspension in a volume ratio of 1:5. The negative control was bacterial cells maintained in the absence of the membranes. The optical density of the bacterial cell suspension at 600 nm (OD600) was employed to evaluate the function of bacterial cells by applying a TECAN reader, measuring 9 wells. The experiments were carried out in 6 replications. The results of the bacterial function test are presented below (Figure 4).  The evaluated membrane showed no bacteriostatic effect on the tested strain and no induction of bacterial cell proliferation compared to the control (bacterial cells cultured in the absence of the membranes). Furthermore, there were no significant differences in bacteria functioning among cultures in the presence of individual membranes and the absence of membranes (Control). However, some authors [31] observed the antimicrobial influence of alginate oligosaccharides.

Assessment of the Transport Properties of the Membranes
Some authors have examined diffusion coefficients for the binary aqueous systems containing sodium alginate [32][33][34]. Nevertheless, the systems involving sodium alginate with perfluorooctyl for the same concentrations were not reported. The sodium alginate in PBS dilute solutions, at 2.2%, alone and combined with perfluorooctyl, were evaluated.
Diffusive permeability was evaluated using a thermodynamic description of diffusive mass transport across a homogenous membrane (Fick's law) and a two-compartment model [35]; for that purpose, the beads produced from (ALG-PFC)net or (ALG) net (as a control) were placed into the solution of model particles. Dextrans of molecular weight 70 and 150 kDa were applied as the model particles. Then, the concentration of the marker was spectrophotometrically evaluated.
The control (ALG) net and (ALG-PFC) net adsorbed Dextran 70 (Dex 70), allowing for about 10% release related to the initial concentration value of 1 mg/mL for 30 min.
The control (ALG) net and (ALG-PFC) net adsorbed Dextran 150 (Dex 150), allowing for about 12% release related to the initial concentration value of 0.250 mg/mL for 30 min, indicating that the evaluated material is permeable for applied markers.
The membranes (ALG-PFC) net and (ALG) net exhibited relative changes in the concentration of model particles during the examination time ( Figures 5 and 6). The changes for particular time intervals of permeability and time product confirmed membrane permeating ability for examined solutes of molecular weight 70 and 150 kDa (Figures 7 and 8). Comparing the thermodynamic behavior of sodium alginate and alginate with PFC, it was observed that PFC involvement does not influence transport through the alginate membrane.
The evaluated membrane showed no bacteriostatic effect on the tested strain and no induction of bacterial cell proliferation compared to the control (bacterial cells cultured in the absence of the membranes). Furthermore, there were no significant differences in bacteria functioning among cultures in the presence of individual membranes and the absence of membranes (Control). However, some authors [31] observed the antimicrobial influence of alginate oligosaccharides.

Assessment of the Transport Properties of the Membranes
Some authors have examined diffusion coefficients for the binary aqueous systems containing sodium alginate [32][33][34]. Nevertheless, the systems involving sodium alginate with perfluorooctyl for the same concentrations were not reported. The sodium alginate in PBS dilute solutions, at 2.2%, alone and combined with perfluorooctyl, were evaluated.
Diffusive permeability was evaluated using a thermodynamic description of diffusive mass transport across a homogenous membrane (Fick's law) and a twocompartment model [35]; for that purpose, the beads produced from (ALG-PFC)net or (ALG)net (as a control) were placed into the solution of model particles. Dextrans of molecular weight 70 and 150 kDa were applied as the model particles. Then, the concentration of the marker was spectrophotometrically evaluated.
The control (ALG)net and (ALG-PFC)net adsorbed Dextran 70 (Dex 70), allowing for about 10% release related to the initial concentration value of 1 mg/mL for 30 min.
The control (ALG)net and (ALG-PFC)net adsorbed Dextran 150 (Dex 150), allowing for about 12% release related to the initial concentration value of 0.250 mg/mL for 30 min, indicating that the evaluated material is permeable for applied markers.
The membranes (ALG-PFC)net and (ALG)net exhibited relative changes in the concentration of model particles during the examination time ( Figures 5 and 6). The changes for particular time intervals of permeability and time product confirmed membrane permeating ability for examined solutes of molecular weight 70 and 150 kDa (Figures 7 and 8). Comparing the thermodynamic behavior of sodium alginate and alginate with PFC, it was observed that PFC involvement does not influence transport through the alginate membrane.      Moreover, the membranes do not delimit the transport of the albumin (70 kDa) and immunoglobulin levels (150 kDa). Moreover, the membranes do not delimit the transport of the albumin (70 kDa) and immunoglobulin levels (150 kDa).

Fourier Transform Infrared (FT-IR) Spectroscopy
The Fourier transform infrared spectra of sodium alginate and sodium alginate with perfluorooctyl are shown in Figure 9. Spectrum of sodium alginate exhibited absorption bands regarding hydroxyl, ether, and carboxylic functional groups. Stretching vibrations of O-H bonds of alginate (which appeared in the range of 3000-3600 cm −1 ) were observed at 3329 cm −1 . The bands observed at 1600 and 1414 cm −1 can be attributed to stretching vibrations of the carboxylate group. The band at 1043 and 1086 cm −1 is attributed to the C-O stretching vibration. After oxygenating factor involvement in the alginate, the bands at 1143 and 1200 cm −1, which can be attributed to C-F bonds, appeared.

Fourier Transform Infrared (FT-IR) Spectroscopy
The Fourier transform infrared spectra of sodium alginate and sodium alginate with perfluorooctyl are shown in Figure 9. Spectrum of sodium alginate exhibited absorption bands regarding hydroxyl, ether, and carboxylic functional groups. Stretching vibrations of O-H bonds of alginate (which appeared in the range of 3000-3600 cm −1 ) were observed at 3329 cm −1 . The bands observed at 1600 and 1414 cm −1 can be attributed to stretching vibrations of the carboxylate group. The band at 1043 and 1086 cm −1 is attributed to the C-O stretching vibration. After oxygenating factor involvement in the alginate, the bands at 1143 and 1200 cm −1, which can be attributed to C-F bonds, appeared. Moreover, the membranes do not delimit the transport of the albumin (70 kDa) and immunoglobulin levels (150 kDa).

Fourier Transform Infrared (FT-IR) Spectroscopy
The Fourier transform infrared spectra of sodium alginate and sodium alginate with perfluorooctyl are shown in Figure 9. Spectrum of sodium alginate exhibited absorption bands regarding hydroxyl, ether, and carboxylic functional groups. Stretching vibrations of O-H bonds of alginate (which appeared in the range of 3000-3600 cm −1 ) were observed at 3329 cm −1 . The bands observed at 1600 and 1414 cm −1 can be attributed to stretching vibrations of the carboxylate group. The band at 1043 and 1086 cm −1 is attributed to the C-O stretching vibration. After oxygenating factor involvement in the alginate, the bands at 1143 and 1200 cm −1, which can be attributed to C-F bonds, appeared. Figure 9. FTIR spectra of sodium alginate (ALG) and sodium alginate with perfluorooctyl (ALG-PFC). Figure 9. FTIR spectra of sodium alginate (ALG) and sodium alginate with perfluorooctyl (ALG-PFC).

Evaluation of Morphology of Oxygenated Membrane and the Cells within the Membrane
In order to characterize the surface topography of the prepared membranes, atomic force microscopy (AFM) was employed. AFM visualization of the applied (ALG-PFC) net layer deposited onto gold-covered mica substrate is presented in Figure 10. The effect of a conglomeration of the polyguluronic acid segments in clusters due to cross-linking can obtain the even structure of the coating layer. As a result, the granular branched structure with an average grain size of 5 nm (based on a particle analysis tool) can be observed. In addition, the root means square roughness parameter for the whole surface was measured to have a 5.5 nm value.

Evaluation of Morphology of Oxygenated Membrane and the Cells within the Membrane
In order to characterize the surface topography of the prepared membranes, atomic force microscopy (AFM) was employed. AFM visualization of the applied (ALG-PFC)net layer deposited onto gold-covered mica substrate is presented in Figure 10. The effect of a conglomeration of the polyguluronic acid segments in clusters due to cross-linking can obtain the even structure of the coating layer. As a result, the granular branched structure with an average grain size of 5 nm (based on a particle analysis tool) can be observed. In addition, the root means square roughness parameter for the whole surface was measured to have a 5.5 nm value. Chemical composition analysis of the cross-linked (ALG-PFC)net samples by X-ray spectrometer (EDX) was performed using an EDX analyzer. (ALG-PFC)net mapping images in the spectra show the characteristic elements for perfluorooctyl and the crosslinking factor. There were mean values obtained: O: 11.88 ± 4.50; F: 0.25 ± 0.01; Ca: 26.42 ± 3.55; Cl: 51.61 ± 6.47. A representative image of the spectrum is presented in Figure 11. The obtained results confirmed the presence of oxygenating and cross-linking factors. Chemical composition analysis of the cross-linked (ALG-PFC) net samples by X-ray spectrometer (EDX) was performed using an EDX analyzer. (ALG-PFC) net mapping images in the spectra show the characteristic elements for perfluorooctyl and the cross-linking factor. There were mean values obtained: O: 11.88 ± 4.50; F: 0.25 ± 0.01; Ca: 26.42 ± 3.55; Cl: 51.61 ± 6.47. A representative image of the spectrum is presented in Figure 11. The obtained results confirmed the presence of oxygenating and cross-linking factors.
When analyzing 'cell' morphology using fluorescence microscopy, it was observed that cells grown in the presence of ALG-PFC and (ALG-PFC) net membranes show the correct morphological picture (Figures 12 and 13). After six days of the culture, we observed numerous cells on the surface of glass coverslips coated with ALG-PFC and (ALG-PFC) net . In addition, the spindle-shaped cells of fibroblastoid features were present on both types of surfaces. When analyzing 'cell' morphology using fluorescence microscopy, it was observed that cells grown in the presence of ALG-PFC and (ALG-PFC)net membranes show the correct morphological picture (Figures 12 and 13). After six days of the culture, we observed numerous cells on the surface of glass coverslips coated with ALG-PFC and (ALG-PFC)net. In addition, the spindle-shaped cells of fibroblastoid features were present on both types of surfaces.   When analyzing 'cell' morphology using fluorescence microscopy, it was observed that cells grown in the presence of ALG-PFC and (ALG-PFC)net membranes show the correct morphological picture (Figures 12 and 13). After six days of the culture, we observed numerous cells on the surface of glass coverslips coated with ALG-PFC and (ALG-PFC)net. In addition, the spindle-shaped cells of fibroblastoid features were present on both types of surfaces.

Conclusions
In summary, PFC involving the alginate layer improved HDF function during the 6 days of the experiment, influencing viability and mitochondrial activity without

Conclusions
In summary, PFC involving the alginate layer improved HDF function during the 6 days of the experiment, influencing viability and mitochondrial activity without delimiting the permeability. Since PFC is immiscible in aqueous solutions, including blood, it can constitute a safety factor of the dressing element at the interface with the wound. Moreover, the layer coating material involving the oxygenating and cross-linking factors topography ensures an even contact surface for interface with the wound. Applying the oxygenating and/or cross-linking factors causes fluctuation of mitochondrial activity during the culture on the modified support patches. Concerning these fluctuations, the systems of modified patches can be recommended for application up to six days (five days of contact of cells with modified patches). Sodium and calcium alginates are widely used as dressings. Although they are not the healing components, such covers do not adhere to seeping wounds, and because they are highly hydrophilic, they absorb exudate and retain moisture, promoting healing. Therefore, the layer coating with applied oxygenating configuration within alginate can involve a therapeutic function to the element of the dressing platform.
Generally, the ideal universal wound dressing still has not been provided; thus, new products have been developed and functionalized to obtain the properties that can influence the wound-healing process. Such a product can be the polyelectrolyte alginate layer coating with an incorporated oxygenating factor, which can be recommended as an element of a dressing system.