Efficacy of Phase I and Phase II Coxiella burnetii Bacterin Vaccines in a Pregnant Ewe Challenge Model

The bacterium Coxiella burnetii can cause the disease Q-fever in a wide range of animal hosts. Ruminants, including sheep, are thought to play a pivotal role in the transmission of C. burnetii to humans; however, the only existing livestock vaccine, namely, Coxevac® (Ceva Animal Health Ltd., Libourne, France), a killed bacterin vaccine based on phase I C. burnetii strain Nine-Mile, is only approved for use in goats and cattle. In this study, a pregnant ewe challenge model was used to determine the protective effects of Coxevac® and an experimental bacterin vaccine based on phase II C. burnetii against C. burnetii challenge. Prior to mating, ewes (n = 20 per group) were vaccinated subcutaneously with either Coxevac®, the phase II vaccine, or were unvaccinated. A subset of pregnant ewes (n = 6) from each group was then challenged 151 days later (~100 days of gestation) with 106 infectious mouse doses of C. burnetii, Nine-Mile strain RSA493. Both vaccines provided protection against C. burnetii challenge as measured by reductions in bacterial shedding in faeces, milk and vaginal mucus, and reduced abnormal pregnancies, compared to unvaccinated controls. This work highlights that the phase I vaccine Coxevac® can protect ewes against C. burnetii infection. Furthermore, the phase II vaccine provided comparable levels of protection and may offer a safer and cost-effective alternative to the currently licensed vaccine.


Introduction
Coxiella burnetii (C. burnetii) is a highly infectious obligate intracellular bacterium that is the etiologic agent of the zoonosis Q-fever. The disease was first identified in abattoir workers in Australia as a febrile illness of unknown origin termed query fever (Q-fever) [1].
individuals with prior C. burnetii exposure [27]. Coxevac ® is a livestock vaccine licensed for use in cattle and goats and is not currently approved for use in sheep [28]. Despite generating high levels of protection, the safety of phase I vaccines is a major issue considering that severe local and systemic reactions can occur post vaccination; it has been documented that Coxevac ® can cause painful injection site reactions, an increase in body temperatures and a decrease in milk yields [29]. Additionally, the manufacture of phase I vaccines involves the culture of C. burnetii at containment level 3 (CL3), which has both cost and human safety issues and has limited the deployment of these vaccines [30]. Vaccines based on phase II antigens can be produced at a lower containment level, CL2, reducing both associated risk and cost; however, these have been shown to be non-protective in goats and guinea pigs [31,32]. Few studies have assessed the impact of phase I vaccines on sheep, and no studies have investigated the protective potential of phase II vaccines in pregnant ewes [33].
The aim of the study presented here was to investigate the efficacy of a phase I vaccine and a phase II vaccine in pregnant ewes experimentally challenged with C. burnetii. Protection was determined by reductions in adverse pregnancy outcomes, shedding of C. burnetii in milk, faeces and vaginal secretions and tissue levels of bacteria. Vaccines used in this study were the commercially available phase I C. burnetii vaccine, Coxevac ® (strain RSA 493/Nine-Mile phase I), and a non-commercial vaccine formulation composed of C. burnetii phase II antigens adjuvanted with Quil-A.

Propagation of C. burnetii Strain
Coxiella burnetii Nine-Mile strain RSA493 was cultured in Buffalo Green Monkey (BGM) cells by colleagues at Wageningen Bioveterinary Research, the Netherlands, as described previously [34]. This strain was originally isolated in Montana, in 1935, from the tick Dermacentor andersoni [35]. Prior to inoculation the concentration of the strain was adjusted to 10 6 infective mouse doses (IMD)/mL. Aliquots were stored at −70 • C until use.

Animals
Adult female 3-year-old Texel-cross ewes, which had previously been vaccinated against Chlamydia abortus (C. abortus), were used in this study. Ewes were initially kept on pasture and then moved to the Moredun High Security Unit (HSU) prior to C. burnetii challenge. In the HSU, all study animals had access to water ad libitum and were fed appropriate volumes of Premium 18 sheep nuts (Harbro, UK) and ad libitum hay. Energise feed mineral lick tubs (Harbro, UK) were provided during the last two weeks of pregnancy. Prior to the start of the study, all ewes were negative for C. burnetii antibodies as determined by enzyme-linked immunosorbent assay (ELISA) detailed in Section 2.5.2.

Immunization and Challenge
The experimental protocol for the immunization study is summarized in Figure 1. Ewes were randomly allocated into three groups and were immunized via the subcutaneous route on two occasions three weeks apart with either 2 mL Coxevac ® (Lot. 0807FG1A, Ceva Animal Health Ltd., Libourne, France) containing formaldehyde inactivated phase I C. burnetii Nine-Mile strain RSA493 plus thiomersal preservative in PBS (Group 1, n = 20), or 2 mL of formalin inactivated phase II C. burnetii Nine-Mile strain (Lot. SAI.CH, Virion/Serion, Würzburg, Germany) containing 1 mg Quil-A (Brenntag Biosector, Frederikssund, Denmark) and thiomersal preservative in PBS (Group 2, n = 20). Phase II C. burnetii was generated following 166 passages in embryonated hen eggs (personal communication, Bioscience Slovakia, Bratislava, Slovakia). To ensure the phase I and phase II bacterin vaccines contained equivalent numbers of C. burneti, quantitative PCR (qPCR) using the Adiavet™ Coxiella Real Time kit (Bio-X Diagnostics, Rochefort, Belgium) was carried out following the manufacturer's instructions. Both vaccines were adjusted to 1.20 × 10 10 Two weeks after the second immunization, oestrus was synchronized in ewes using Chronogest CR 20 mg controlled flugestone acetate release vaginal sponges (MSD Animal Health, Milton Keynes, UK), and ewes were mated two weeks later. A further 11 weeks later, ewes were ultrasound scanned to determine pregnancy status and six ewes from each of Groups 1-3, weighted for parity, were selected for C. burnetii challenge. Each group contained five twin-bearing ewes and one triplet-bearing ewe. At day 102 post mating, these ewes were challenged in the Moredun HSU with 10 6 IMD of C. burnetii Nine-Mile strain RSA493 via the subcutaneous route.

Assessment of Post-Vaccination Reactogenicity
The rectal temperatures of all ewes were taken immediately prior to each of the two vaccinations and then again at 24 h post vaccination, when animals were also inspected by a veterinarian for the presence of injection site reactions.

Sample Collection
Blood samples were collected from all ewes for serological analysis on a weekly basis for the first eight weeks after the first immunization, then every second week until pregnancy scanning. Ewes and surviving lambs were euthanized by overdose of sodium pentobarbitone at 32 weeks relative to the first immunization (~4 weeks post lambing) for post-mortem analysis. For challenged ewes only, blood samples were also collected weekly from the time of challenge until post-mortem. Milk, faeces and vaginal swab samples were collected from challenged ewes on days 0, 1, 2 and 3, relative to lambing/abortion, and weekly thereafter until post-mortem, for C. burnetii detection by qPCR. Placental samples, including at least one cotyledon and one inter-cotyledonary membrane, were collected at lambing/abortion. To determine the tissue distribution of C. burnetii in lambs and ewes, samples of spleen and liver were collected from aborted foetuses, stillborn lambs and lambs sacrificed at the end of the study. Samples of liver, spleen, uterine body, udder, supra-mammary lymph node (SMLN) and mammary gland were collected from sacrificed ewes. Two weeks after the second immunization, oestrus was synchronized in ewes using Chronogest CR 20 mg controlled flugestone acetate release vaginal sponges (MSD Animal Health, Milton Keynes, UK), and ewes were mated two weeks later. A further 11 weeks later, ewes were ultrasound scanned to determine pregnancy status and six ewes from each of Groups 1-3, weighted for parity, were selected for C. burnetii challenge. Each group contained five twin-bearing ewes and one triplet-bearing ewe. At day 102 post mating, these ewes were challenged in the Moredun HSU with 10 6 IMD of C. burnetii Nine-Mile strain RSA493 via the subcutaneous route.

Assessment of Post-Vaccination Reactogenicity
The rectal temperatures of all ewes were taken immediately prior to each of the two vaccinations and then again at 24 h post vaccination, when animals were also inspected by a veterinarian for the presence of injection site reactions.

Sample Collection
Blood samples were collected from all ewes for serological analysis on a weekly basis for the first eight weeks after the first immunization, then every second week until pregnancy scanning. Ewes and surviving lambs were euthanized by overdose of sodium pentobarbitone at 32 weeks relative to the first immunization (~4 weeks post lambing) for post-mortem analysis. For challenged ewes only, blood samples were also collected weekly from the time of challenge until post-mortem. Milk, faeces and vaginal swab samples were collected from challenged ewes on days 0, 1, 2 and 3, relative to lambing/abortion, and weekly thereafter until post-mortem, for C. burnetii detection by qPCR. Placental samples, including at least one cotyledon and one inter-cotyledonary membrane, were collected at lambing/abortion. To determine the tissue distribution of C. burnetii in lambs and ewes, samples of spleen and liver were collected from aborted foetuses, stillborn lambs and lambs sacrificed at the end of the study. Samples of liver, spleen, uterine body, udder, supra-mammary lymph node (SMLN) and mammary gland were collected from sacrificed ewes.

Preparation of Clinical Samples
Following collection, blood samples were left to clot overnight at room temperature. Samples were centrifuged for ten minutes at 2000× g. Serum was removed using a sterile pastette into a labelled 2 mL screw cap tube (Sarstedt, Nümbrecht, Germany). All samples, including tissue samples, were stored at −70 • C for future downstream processing and analyses.

DNA Extraction
DNA from approximately 200 mg of each faecal sample was extracted using a QIAamp DNA stool kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. DNA was extracted from milk, vaginal swab, and tissue samples using a DNeasy Blood and Tissue Kit (Qiagen, Germany), with any modifications outlined below. Proteinase K (20 µL) and buffer ATL (180 µL) was added to 200 µL milk samples, which were heated at 56 • C for one hour, after which the standard protocol was resumed. Vaginal swabs were added to 1.5 mL screw cap Eppendorf tubes containing 400 µL buffer ATL and 20 µL Proteinase K. Samples were vortexed and lysed at 56 • C for 10 min, and then 400 µL of buffer AL was then added to samples, after which the standard protocol was resumed. All tissue samples were lysed overnight at 56 • C. An extraction control, comprising 200 µL of sterile distilled water, was included in every run. To elute DNA, 50 µL of buffer AE (Qiagen, Germany) was used for all samples. Samples were stored at −70 • C prior to analysis.

Detection of Abortifacient Agents
To determine if abnormal lambing outcomes were attributable to C. burnetii, qPCR and ELISA assays targeting C. burnetii, C. abortus and Toxoplasma gondii (T. gondii), common ovine abortifacient agents, were carried out on a defined subset of samples:

qPCR Detection of Abortifacient Agents
To detect the presence of C. burnetii DNA, all samples were subject to qPCR using an assay which targeted the multi-copy-number insertion sequence IS1111 [36,37]. To detect C. abortus DNA all placenta samples (cotyledon and inter-cotyledonary membrane) and vaginal swabs collected on day 0 post lambing were subject to qPCR, targeting the major outer membrane protein (MOMP) of C. abortus [38]. To detect T. gondii DNA, qPCR on a 529bp repeat element was carried out on all placenta samples (cotyledon and intercotyledonary membrane) and on lamb liver and spleen samples [39]. Primers and probes used in this study are detailed in Table 1. Briefly, the final 20 µL reaction volume for each qPCR consisted of 10 µL QuantiNova master-mix (Qiagen, Germany), the required concentrations of each forward and reverse primers and probes as stated in Table 1, 1x QN ROX™ reference dye (Qiagen, Germany), RNase and DNase free water (Invitrogen, Paisley, UK) and 3 or 5 µL of extracted DNA sample. For C. burnetii and T. gondii 5 µL of extracted DNA was used, while 3 µL was used for C. abortus. Two negative controls were included in each qPCR run: (i) a no-template control consisting of molecular grade water; (ii) the elution from the negative extraction controls. For T. gondii qPCRs, 0.01 fg competitive internal amplification control (CIAC) was also included in each PCR reaction. For C. burnetii qPCRs, a positive control sample consisting of genomic DNA prepared from C. burnetii Nine-Mile strain RSA493 was included in each PCR run. DNA was amplified using an ABI Prism ® 7000 Sequence Detection System (Thermo Fisher Scientific, Waltham, USA) in duplicate, for every sample, using the following conditions: C. burnetii qPCR: 1 cycle at 95 • C for 120 s followed by 45 cycles of 95 • C for 5 s and 60 • C for 5 s; C. abortus qPCR: 1 cycle at 95 • C for 10 min followed by 45 cycles of 95 • C for 15 s and 60 • C for 1 min; T. gondii qPCR: 1 cycle at 95 • C for 10 min followed by 45 cycles of 95 • C for 10 s, 58 • C for 20 s, and 72 • C for 20 s. Based on the positive control sample, intra-assay co-efficient of variation (CV) for the C. burnetii qPCR assay was in the range of 0.00-2.06%, and inter-assay CV was 5.9%. Linearity of the C. burnetii qPCR assay, PCR efficiency and limit of detection were determined by testing serial dilutions of the positive control sample. The correlation coefficient of the assay was in the range of 0.993-0.998, PCR efficiency was in the range of 100.1-101.36%, and the limit of detection was 2.5 × 10 1 Genome Equivalents/PCR reaction. As the qPCR target for the C. burnetii qPCR (IS1111) is a multi-copy gene, data was not analysed quantitatively; rather, samples were deemed positive for C. burnetii only if both duplicates from each sample resulted in a positive qPCR signal.

Serology
Coxiella burnetii ELISA Serum samples were tested for the presence of antibodies against C. burnetii using an ELISA (IDEXX Q-Fever antibody test; IDEXX, UK) following the manufacturer's instructions. A positive and a negative control was supplied by the manufacturer and was included in every assay. The percentage positivity (PP) of each sample was calculated as: where ODs is the optical density (OD) of the sample, ODnc is the average OD of the negative controls and ODpc is the average OD of the positive controls. Sera were considered positive if the PP was equal to or greater than 40%, suspect if the PP was between 30 and 40% and negative if the PP was less than 30%.
Chlamydia abortus ELISA Sera collected pre-tupping, at lambing and three weeks post lambing were tested using an in-house C. abortus specific rOMP90B-3 indirect ELISA, as described previously [40]. Results were calculated as a percentage positivity, in relation to positive and negative control sera. Sera were considered positive (+) if the OD percentage was greater than 60%, ambiguous (−/+) if the OD percentage was between 50 and 60% and negative (−) if the OD percentage was less than 50%.

LPS Extraction
To confirm that the LPS of the phase II vaccine used in this study was truncated, LPS was purified using an LPS Extraction kit (Abcam, Cambridge, UK), according to the manufacturer's instructions, with the following modifications: the lyophilised phase II antigen (Virion/Serion, Germany) was weighed, and ten volumes of lysis buffer were added to the antigen preparation. Samples were kept in an ice bath and individually sonicated (MISONIX S-3000, Cole-Parmer) three times for 30 s, in a continuous pulse at 2-10 watts and incubated on ice for 10 min. Samples were centrifuged for 10 min at 4 • C at 2500× g. The supernatant was transferred to a clean 1.5 mL Eppendorf tube, and 0.1 mg/mL Proteinase K was added. Samples were heated at 60 • C for 60 min and then centrifuged at 4 • C for 10 min at 2500× g. Supernatant was transferred to a clean 1.5 mL Eppendorf and stored at −20 • C prior to prior to further analysis.
2.6.2. Visualization of C. burnetii LPS LPS samples extracted from C. burnetii phase II antigen were analysed by SDS-PAGE using the NuPAGE ® electrophoresis system (Thermo Fisher Scientific, USA). Briefly, samples were prepared in NuPAGE LDS sample buffer with reducing agent and heated to 70 • C for 10 min prior to loading on a NuPAGE™ 4-12% Bis-Tris gel (Thermo Fisher Scientific, USA). Gels were run in MES SDS running buffer and included an LPS standard and a SeeBlue™ Plus2 Pre-Stained Protein Ladder (Thermo Fisher Scientific, USA). Following electrophoresis, in gel LPS was stained using a SilverQuest™ staining kit (Invitrogen, USA) following the manufacturer's instructions.

Statistical Analysis
To assess the effect of treatment group (1-3) on rectal temperature, linear regression models were fitted to temperature 24 h post first and second vaccinations separately, with baselines temperatures at vaccination and treatment groups used as explanatory variables (no statistically significant differences between treatment groups were observed at the time of vaccination in any case). Post hoc pairwise comparisons between treatment groups were derived from the model fits, with the resulting p-values being adjusted for false discovery rate (FDR) using the Benjamini-Hochberg method [41].
The differences in mean antibody response by treatment group (ELISA data expressed as percentage positivity (PP)) were analysed using a generalized additive model (GAM). The model was fitted by the restricted maximum likelihood (REML) method to log10 (PP + 1) as response variable, with identify link function and Gaussian errors. As explanatory terms, the model included treatment group and spline-based smooth terms (one per treatment group) to account for the non-linearity of the relationship of the response with time. Autocorrelation of the measurements on the same animals over time was accounted for by considering a continuous autoregressive correlation structure of order 1 (CAR (1)). Heterogeneous variances by treatment group were allowed. Differences between the linear terms for each treatment group were examined using Wald tests, and the resulting p-values were FDR-adjusted.
The real-time PCR-based cumulative positive/negative outcome data were analysed for each type of sample (faecal, milk and vaginal swab) using binomial generalized linear models (GLM) with a logit link function, including treatment group as main effect. Model parameters were estimated by the maximum likelihood method including a bias-reduction correction [42] to accommodate the lack of variation of the outcome within the vaccinated groups.
Finally, the association of treatment group with lamb survival and normal pregnancy was statistically assessed by applying a Fisher's exact test to the combined treatment groups compared to the control.
All statistical analyses were performed using the R system for statistical computing v3 [43]. Significance tests were assessed at the usual 5% significance level.

Assessment of Post-Vaccination Reactogenicity
Post vaccination, no notable injection site reactions were observed in any animal. However, a statistically significant effect of treatment group on temperature was detected at 24 h following both the initial vaccination and second vaccination (p < 0.001).
Rectal temperatures are shown in Table S1. At 24 h after the first vaccination, the rectal temperatures of ewes in Group 3 (unvaccinated) were statistically significantly lower than either of the two vaccinated groups (p < 0.001). The rectal temperature of Group 3 ewes were 0.68 and 0.76 • C lower, on average, than Group 1 and 2, respectively. At 24 h after the second vaccination, the rectal temperatures of ewes in Group 2 (phase II vaccine group) were statistically significantly higher than those in Group 1 (Coxevac ® vaccinated) and Group 3 (unvaccinated) (p < 0.001). The rectal temperature of Group 2 ewes were 0.79 and 1.00 • C higher, on average, than Group 1 and 3, respectively.

Serological Response to Vaccination and C. burnetii Challenge
For each group, the average serological ELISA response following vaccination and challenge is shown in Figure 2. The C. burnetii-specific antibody response in the unvaccinated group (Group 3) was statistically significantly lower across the whole time period compared to either of the two vaccinated groups (p < 0.001). Generally, the antibody response, post vaccination and post challenge, did not statistically significantly differ between Groups 1 and 2 (p > 0.05), although an examination of fitted trends, Figure S1, suggested a higher antibody response in Group 2 (phase II vaccine group) between day 21 and day 49 (i.e., the four-week period immediately after the second vaccination) compared to Group 1 (Coxevac ® /phase I vaccine group). According to the 40% positivity threshold of the IDEXX Q-Fever antibody test, at day 35, all ewes in Groups 1 and 2 were seropositive for C. burnetii, except for one animal in Group 1 (ewe no. 9704): this ewe seroconverted at low levels following vaccination and antibody levels consistently remained below the positivity threshold. Following a post-vaccination peak at day 35, the average antibody levels in Groups 1 and 2 declined gradually to below the ELISA positivity threshold prior to challenge. Post C. burnetii challenge (day 151), average antibody levels in both Groups 1 and 2 increased sharply, peaking on day 175 before decreasing gradually to 53% and 40%, respectively, at the end of the trial (day 224). No seroconversion was observed in Group 3 (unvaccinated) ewes at any point prior to challenge (day 151). Post challenge, seroconversion was observed in five out of the six ewes in Group 3 (ewe no. 9668 did not seroconvert, Figure S2), although all antibody titres were below the ELISA positivity threshold except for one of the five ewes (no. 22164). One animal in Group 3 (no. 23501) exhibited a large increase in serum levels of C. burnetti specific antibodies post lambing (day 210), which was not apparent in the other ewes in this group (see Figure S2).

Shedding of C. burnetii in Milk, Faecal and Vaginal Swab Samples
Samples were collected daily from C. burnetii challenged ewes on days 0, 1, 2 and 3 post lambing and at weekly time points thereafter until the completion of the trial (~4 weeks post lambing). Groups initially consisted of six ewes each; however, five animals were eventually available in groups 1 and 2, due to abortion in one ewe in Group 1 at 115 days of gestation, due to C. abortus infection (see Section 3.6), and the death of one ewe in Group 2, due to complications associated with a vaginal prolapse. Results are summarized

Shedding of C. burnetii in Milk, Faecal and Vaginal Swab Samples
Samples were collected daily from C. burnetii challenged ewes on days 0, 1, 2 and 3 post lambing and at weekly time points thereafter until the completion of the trial (~4 weeks post lambing). Groups initially consisted of six ewes each; however, five animals were eventually available in groups 1 and 2, due to abortion in one ewe in Group 1 at 115 days of gestation, due to C. abortus infection (see Section 3.6), and the death of one ewe in Group 2, due to complications associated with a vaginal prolapse. Results are summarized in Tables 2-4. Table 2. Presence (+) or absence (-) of C. burnetii DNA in milk samples. Samples were collected on day of lambing (0) and then for three consecutive days (1, 2 and 3). Samples were then collected weekly for 3 weeks, with a final sample set collected immediately prior to post-mortem (PM). -  -   Unvaccinated controls   9668  ---NA  -+  +  -21898  -NA  -+  -+  +  -22164  ---NA  --+  -23501  +  +  +  +  +  +  --23538  -----+  --22357  ----+

C. burnetii Shedding at Weekly Sampling Points
Following the completion of lambing, positive qPCR outcomes over three consecutive weekly sample points and at post-mortem in animals across the three treatment groups were analysed. For faecal samples, the vaccinated groups (Group 1 and 2) had a statistically significantly lower number of positives than the unvaccinated control group (Group 3) (p = 0.030). For milk samples, the vaccinated groups (Group 1 and 2) had a statistically significantly lower number of positives than the unvaccinated control group (Group 3) (p < 0.001). For vaginal swab samples, the vaccinated groups (Group 1 and 2) had a statistically significantly lower number of positives than the unvaccinated control group (Group 3) (p = 0.002). There were no statistically significant differences between the two vaccinated groups for any of the samples tested.

Presence of C. burnetii DNA in Tissue Samples of Ewes and Lambs at Post-Mortem
No C. burnetii DNA was detected in any of the collected tissue samples in animals belonging to either of the vaccinated groups (Groups 1 and 2). In the unvaccinated ewes (Group 3), C. burnetii DNA was only detected in the SMLN of one ewe (no. 22357) and in the udder, uterus, and placenta (cotyledon and inter-cotyledonary membrane) of another ewe (no. 23501). C. burnetii DNA was only detected in the liver of one healthy lamb from an unvaccinated ewe (no. 23501, Group 3).

Lambing Outcomes
The lambing outcomes are shown in Table 5. No abortions attributed to C. burnetii challenge were observed during this study. Offspring from ewe no. 9880 and no. 23647 were excluded from the final lambing outcome analysis. Ewe 9880, which aborted triplets, was serologically and qPCR positive for C. abortus as detailed in Section 3.6. Ewe 23647 died because of septicaemia subsequent to a vaginal prolapse prior to lambing with no abortifacient agents detected in ewe or lamb tissue samples or in a vaginal swab collected post-mortem.  The effect of the vaccines on the proportions of lambs surviving and of abnormal pregnancies are shown in Tables 6 and 7, respectively. The analysis of lamb survival showed no statistically significant difference between the vaccinated groups (Group 1 and 2) and the unvaccinated group (p = 0.100). A normal pregnancy was defined as all lambs born healthy and which survived to the end of the trial period. A statistically significantly higher number of abnormal pregnancies was noted in the unvaccinated group (Group 3) compared to the vaccinated groups (Group 1 and 2) (p = 0.008). There were no significant differences in lambing outcomes between the vaccinated groups (Group 1 and 2).

Chlamydia abortus and Toxoplasma gondii Status
The animals used in this study were previously used in an experimental C. abortus vaccine trial. To rule out whether abnormal lambing outcomes observed in this study were associated with C. abortus or T. gondii infection, serology was performed for both pathogens at multiple time-points: pre-tupping, lambing and at three weeks post lambing (C. abortus only), and qPCR performed on placental and lamb tissues at post-mortem. The results are summarized in Tables S2 and S3. All the ewe and lamb samples tested for T. gondii were qPCR negative. Pre-tupping 2/6, 1/6 and 3/6 ewes were positive for T. gondii antibodies in Groups 1, 2 and 3, respectively, with antibodies levels remaining constant throughout the trial. All ewe samples tested from Group 2 and 3 were qPCR negative for C. abortus. Except for one ewe (no. 21996) all ewes from Group 1 were qPCR positive for C. abortus in vaginal mucus (day 0) and of these positive animals three were also qPCR positive for placental samples. Pre-tupping 2/6, 4/6 and 2/6 ewes were positive for C. abortus antibodies in Groups 1, 2 and 3, respectively. Of these, C. abortus antibody levels remained consistent throughout the study apart from one ewe in Group 1 (no. 9880) in which antibody levels increased at each time-point, and one ewe in Group 3 (no. 22357) in which antibody levels increased slightly between the first and second but not the second and third time-points. Ewe no. 9880, which aborted triplets on day 115 of gestation, was PCR positive for C. abortus in placental tissues (cotyledon and inter-cotyledonary membrane) and vaginal mucus, collected on the same day, but not for C. burnetii or T. gondii, indicating that the cause of abortion in this ewe was most likely C. abortus. Ewe no. 22357 gave birth to triplets of which one was weak and was euthanized at 24 h. Tissue samples from ewe 22357 and its offspring were PCR negative for T. gondii and C. abortus, however the SMLN tested positive for C. burnetii. Milk, faecal and vaginal swab samples collected from this ewe were positive for C. burnetii at 5/8, 1/8 and 5/8 sampling time-points, respectively, indicating that the abnormal lambing outcome was more likely to be associated with C. burnetii infection.

Characterisation of C. burnetii Phase II LPS
Phase II C. burnetii possess a truncated form of LPS [23]. LPS extracted from the phase II antigen was run on an SDS-PAGE gel, which included an LPS standard and protein ladder, and was then silver stained. For the phase II vaccine, two low-molecular-weight bands of approximately 4 and 7 kDa were present, as indicated in Figure S3, which is consistent with truncated forms of LPS reported by Beare et al. [26].

Discussion
Vaccination of pregnant ewes with either of the C. burnetii study vaccines, phase I (Coxevac ® ) or phase II, induced a strong humoral response that was reactivated post challenge and provided comparable high levels of protection against C. burnetii challenge compared to unvaccinated controls. Immunization with either vaccine caused complete cessation of shedding in faecal and milk samples, significantly reduced shedding in vaginal mucus and resulted in a higher proportion of normal pregnancies, compared to unvaccinated controls.
As reported in other ruminant species [29,44] vaccination with Coxevac ® caused a statistically significant increase in the mean rectal temperature of ewes in Group 1 at 24 h post vaccination compared to unvaccinated animals (Group 3). Compared to the unvaccinated ewes, the mean rectal temperature of animals in Group 2 (phase II vaccinated) also increased 24 h post initial vaccination and at 24 h after the second vaccination. These results indicate that a phase II vaccine can also cause increases in rectal temperatures, similarly to Coxevac ® ; however, these are expected to be transient. Furthermore, the adjuvant Quil-A has previously been shown to induce a pyrogenic response, which may account for the small, yet statistically significant, increase in the mean rectal temperature observed in Group 2 ewes [45]. The complete lack of injection site reactions in these naïve animals is encouraging for the development of a phase II C. burnetii vaccine, although testing in animals previously exposed to C. burneiti is required to fully assess the potential for reactogenicity.
The results observed in sheep inoculated with the phase I vaccine Coxevac ® , particularly reductions in shedding following vaccination, are similar to those observed in several goat vaccination studies [30,31,46]. In Europe, Coxevac ® is currently licensed for use in cattle and goats [44]; however, this study illustrates that high levels of protection are also afforded in sheep. Arricau-Bouvery et al. [31] examined the impact of phase I and phase II C. burnetii vaccines in goats, reporting high levels of protection in goats vaccinated with Coxevac ® ; however, the phase II vaccine used was found to be completely ineffectual [31]. The same authors [31] used the Chlamyvax FQ phase II inactivated vaccine (Merial, France) [47]; however, production of this vaccine has since terminated, and an alternative phase II vaccine formulation was used in the current study that may account for the differences observed. One major difference is that the phase II vaccine used in the current study was adjuvanted with the saponin-based adjuvant Quil-A, whereas Chlamyvax FQ used an oil emulsion adjuvant system. As saponins are known to effectively induce cell-mediated immune (CMI) responses to inactivated antigens [48] and CMI responses have been shown in mice to be required for effective clearance of C. burnetii [49], it may be that the protection observed with the phase II study vaccine was due to more effective CMI induction compared to Chlamyvax FQ. Alternatively, the double LPS band observed in the phase II LPS extract may indicate that the phase II antigen is of intermediate virulence, comparable to the NMC strain.
Moos and Hackstadt [21] reported that the pyrogenic response and seroconversion induced by the NMC strain in guinea pigs was analogous to that of animals infected with phase I organisms, although the strain was unable to persist in vivo [22]. These results are similar to this study whereby immunization with either vaccine, phase I or phase II, induced a strong, homologous, serological response in ewes. Previously, the truncated LPS of the NMC variant, which displayed no serological reactivity to phase I, was recovered from the Australian QD strain of C. burnetii following 177 passages in eggs [21]. The Nine-Mile phase II antigen used in this study was prepared following 166 passages in embryonated hen's eggs, and it is possible that a mutant with intermediate virulence and a novel LPS profile may have arisen during production.
Beare et al. [26] examined LPS phase transition in multiple phase I strains at passage 2, 10, 20 and 30, noting that each contained a unique LPS profile at passage 2, similar to previous reports [21,23,26,50]. Beare et al. [26] reported apparent reversion to an intermediate form of LPS following 30 passages of the phase I strain S Q217, accompanied by a decrease in phase II LPS [26]. Furthermore, following successive passage, an upper phase II LPS (~6 kDa), not recognized by anti-phase II antibodies, was observed in the Nine-Mile (RSA363) and Dugway (7E65-68) strains alongside the lower phase II LPS (~3 kDa) [26]. Another study also noted this upper phase II form in the Priscilla strain, which was still present following 90 passages [51]. Extraction of LPS from the phase II antigen used in this investigation revealed a double LPS band exhibiting a similar upper and lower band profile. These upper LPS bands may represent an alternate intermediate LPS, facilitating recognition of C. burnetii by pathogen recognition receptors on host dendritic cells, thus inducing a protective immune response.
Additionally, or alternatively, physiological and/or immunological differences between the ovine and caprine species [52,53] may also contribute towards these disparate results. Studies have shown that sheep are less susceptible to the pathogenic effects of C. burnetii infection than goats, with a higher seroprevalence generally reported in the latter [7,54]. Furthermore, abortions and production of weak offspring are more common in goats, with sheep less likely to display clinical signs of Q-fever [2,55]. A major difference between the C. burneiti ovine and caprine models appear to be the serological response of unvaccinated animals to challenge. Arricau-Bouvery et al. [31] reported there was a large and sustained increase in C. burnetii specific antibodies in unvaccinated goats post challenge; however, in the current study, the increase in specific antibody titres in unvaccinated sheep post challenge was marginal and generally began to decline towards the end of the trial. These discrete responses could indicate differences in innate immunity between these two ruminant species following C. burnetii infection, which may facilitate earlier control and/or clearance of the bacteria in sheep compared to goats, explaining the dampened humoral response observed in unvaccinated animals in this study. A comparison of the challenge dose between this study and that of the goat study conducted by Arricau-Bouvery et al. [31] further exemplifies differences in susceptibility to C. burnetii between these two species. In the current experimental trial, ewes were challenged with an IMD of 10 6 , whereas in the goat study animals were given an IMD of 10 4 C. burnetii [31]. Despite sheep in this investigation receiving 100-fold more bacteria, no abortions due to C. burnetti were observed, and ewes did not display any clinical symptoms of Q-fever, in contrast to infected goats [31].
The ewes utilized during the current study were also tested for C. abortus and T. gondii, common ovine abortifacient agents. The serological results for T. gondii indicated that at least one ewe from every group had previously been exposed to the parasite, although the stability of the ELISA results over time, and the negative tissue qPCR results suggested the study animals were not harbouring an active T. gondii infection. As animals were recruited from a C. abortus vaccine study, it was unsurprising that multiple ewes from each group were serologically positive for C. abortus; however, evidence of an active chlamydial infection was only apparent for one ewe in Group 1 (no. 9880). This animal aborted triplets, approximately 4 weeks prior to lambing, and was qPCR positive for C. abortus in the placental tissues and vaginal mucus. Ewe no. 22357 exhibited a small increase in C. abortus antibody titres; however, the causative agent of the weak lamb borne to this animal was judged to be C. burnetii. C. abortus was not detected in placental tissue or vaginal mucus of this ewe, whereas C. burnetii was present in the SMLN and in milk and vaginal mucus at multiple sampling time points.
The results of this study and others highlight that, even in the absence of abortion, C. burnetii should still be considered as a threat to sheep due to potential increases in abnormal pregnancies [2]. Interestingly, one ewe in Group 3 (no. 23501, unvaccinated), which seroconverted at low levels post challenge, exhibited a strong reactivation of C. burnetii specific antibodies post lambing (day 210). Ewe number 23501 was the only unvaccinated animal to consistently shed C. burnetii in milk and vaginal mucus samples and was the only unvaccinated ewe to give birth to offspring that were all registered as healthy. Ewe 23501 exhibited high levels of bacterial shedding and displayed the highest antibody response of all unvaccinated animals indicating a possible positive correlation between bacterial load and serology. Furthermore, the only lamb in this study in which C. burnetii DNA was detected was the progeny of ewe 23501. Studies have shown that ruminants infected with C. burnetii can produce healthy offspring or may even give birth to both healthy and dead young, as observed in this study, indicating that infection does not necessarily lead to negative pregnancy outcomes [56]. It is noteworthy that multiple human Q-fever outbreaks attributed to sheep have arisen in cases where no ovine abortions were reported [57,58]. Roest et al. [56] reported that C. burnetii was initially detected in the organs of kids, from infected goats, but at day 28 post parturition, the bacterium was undetectable. In the current study any surviving lambs were culled between 23 and 29 days post parturition, and these later sampling time-points may explain why C. burnetii DNA was only identified in the liver of one lamb; future studies may benefit from earlier sampling points. Ewe serum samples from all three groups were tested for bacteraemia and were consistently negative throughout the trial, similarly to results reported in C. burnetii infected goats [56].
Few studies have examined protection elicited by the phase I vaccine Coxevac ® in sheep; however, Brooks et al. [33] carried out a similar study in which pregnant ewes were vaccinated with either a formalin-inactivated whole-cell phase I Henzerling strain vaccine or a chloroform methanol residue vaccine of the Nine-Mile strain. After challenge, both phase I vaccines provided protection and reduced, but did not completely eliminate, environmental shedding, similarly to results reported in the current study.
Serological C. burnetii ELISA testing is often used for diagnosis and monitoring of ruminants. The results from the current study indicate that infected animals may seroconvert at low levels, or not at all, despite shedding bacteria in faeces, milk and vaginal mucus. This lack of correlation between seropositivity and shedding has been observed previously in ovine, bovine and caprine studies [10,46,59,60]. The results of the current study and those of others highlight that serology alone is not a comprehensive tool for detection of Q-fever in individual sheep. Furthermore, surveillance of C. burnetii in ruminants could be hampered by temporal shedding patterns exhibited in milk, faecal and vaginal mucus samples [10,46]. Except for one ewe (no. 23501) in Group 3, all non-vaccinated/challenged ewes exhibited transient shedding of the bacterium in milk, faeces and vaginal swab samples. A requirement for multiple time-point sample collection and possible disassociation between serology and shedding underscores the need for improved Q-fever detection and control methods.
The cost and safety issues, both for humans and animals, associated with the production and/or use of phase I C. burnetii vaccines necessitate the development of a novel, safe and effective Q-fever vaccine. The findings presented here, exhibiting comparable results for phase I and phase II vaccines in a pregnant ewe challenge model, highlight that a phase II vaccine formulation may be an appropriate alternative. Further investigation including repetition of this vaccine/challenge trial in a pregnant goat model would be extremely useful to validate the utility of these results and assess how they may be applied in the wider context in the control of Q-fever. Naturally, both humans and animals usually become infected with C. burnetii following inhalation of contaminated spores [11]. The animals in this study were experimentally infected via the subcutaneous route, an unnatural method of infection, which may have impacted results. Roest et al. [56] examined the impact that three different routes of inoculation had on experimentally infected goats. Intranasal inoculation resulted in increased bacterial loads in the placenta and caused abortions at earlier time-points compared to subcutaneous or oral inoculation routes [56]. In sheep the influence of infection route upon dissemination and excretion of the bacterium has not been investigated. As with goats, the intranasal route may improve bacterial colonization and investigation of this technique in sheep combined with the vaccines utilized in this study would more closely reflect natural conditions.

Conclusions
Both study vaccines, phase I and phase II, induced a strong humoral response in sheep and demonstrated protection again C. burnetii, as measured by reduced bacterial shedding and production of healthy lambs. Previously, Q-fever was identified as an occupational hazard, restricted to those working closely with ruminants. The unanticipated Dutch outbreak has highlighted the threat of C. burnetii to public health and underscores the need for improved control strategies. This study demonstrated that the commercially available phase I vaccine Coxevac ® is highly efficacious in sheep and implementation into ovine vaccination programs could help to reduce human Q-fever cases. Additionally, it is proposed that the phase II vaccine used in this trial may offer a safer and more cost-effective alternative to the current Q-fever vaccines.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/vaccines11030511/s1, Figure S1. Plot of individual antibody response values alongside the trends fitted by the generalized additive model for each group; Figure S2. Serological ELISA responses of challenged ewes only, following vaccination and C. burnetii challenge; Figure S3. Phase II C. burnetii antigen LPS extract; Table S1: Rectal temperatures of ewes at primary and secondary vaccination; Table S2: Presence or absence of T. gondii and C. abortus antibodies present in ewe serum samples at pre-tupping, lambing and for C. abortus only at 3 weeks post lambing; Table S3. Presence or absence of C. abortus DNA in milk vaginal swab (VS) samples on day 0 of lambing and in placenta samples, inter-cotyledonary membrane (A) and cotyledon (B).

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.