Development of a T Cell-Based COVID-19 Vaccine Using a Live Attenuated Influenza Vaccine Viral Vector

The COVID-19 pandemic emerged in 2020 and has caused an unprecedented burden to all countries in the world. SARS-CoV-2 continues to circulate and antigenically evolve, enabling multiple reinfections. To address the issue of the virus antigenic variability, T cell-based vaccines are being developed, which are directed to more conserved viral epitopes. We used live attenuated influenza vaccine (LAIV) virus vector to generate recombinant influenza viruses expressing various T-cell epitopes of SARS-CoV-2 from either neuraminidase (NA) or non-structural (NS1) genes, via the P2A self-cleavage site. Intranasal immunization of human leukocyte antigen-A*0201 (HLA-A2.1) transgenic mice with these recombinant viruses did not result in significant SARS-CoV-2-specific T-cell responses, due to the immunodominance of NP366 influenza T-cell epitope. However, side-by-side stimulation of peripheral blood mononuclear cells (PBMCs) of COVID-19 convalescents with recombinant viruses and LAIV vector demonstrated activation of memory T cells in samples stimulated with LAIV/SARS-CoV-2, but not LAIV alone. Hamsters immunized with a selected LAIV/SARS-CoV-2 prototype were protected against challenge with influenza virus and a high dose of SARS-CoV-2 of Wuhan and Delta lineages, which was confirmed by reduced weight loss, milder clinical symptoms and less pronounced histopathological signs of SARS-CoV-2 infection in the lungs, compared to LAIV- and mock-immunized animals. Overall, LAIV is a promising platform for the development of a bivalent vaccine against influenza and SARS-CoV-2.


Introduction
Novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) emerged in China in December 2019 [1,2] and by May 2022 has caused over half a billion cases of acute respiratory disease with over six million fatalities globally [3]. High transmissibility of the virus and the emergence of new SARS-CoV-2 antigenic variants have led to a tremendous global public health problem. To mitigate the coronavirus disease 2019 (COVID-19) pandemic, multiple vaccine candidates based on traditional and completely new technologies have been developed and licensed at an unprecedented speed (reviewed in [4,5]). A recent meta-analysis of the performance of COVID-19 vaccines in real-world settings demonstrated their high effectiveness against SARS-CoV-2-related diseases [6]. The vast majority

Peptides
The influenza Len/17-specific peptide (NP 366-374 ASNENMDTM) was chemically synthesized by Almabion Ltd. (Voronezh, Russia). The SARS-CoV-2-specific peptides (Table S1) were chemically synthesized by IQ chemicals Inc. (Saint Petersburg, Russia). The purity of the obtained peptides was at least 95%, which was confirmed by analytical electrophoresis, analytical reverse phase high performance liquid chromatography and mass spectrometric analysis (MALDI). The CEF Control Peptide Pool was purchased from AnaSpec (Cambridge, UK). PepTivator SARS-CoV-2 Prot_N and PepTivator SARS-CoV-2 Prot_S were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). The peptides were reconstituted in dimethyl sulfoxide to a concentration of 1 mM and stored at −70 • C in single-use aliquots.

Designing of SARS-CoV-2 T-Cell Cassettes for Insertion into LAIV Genome
The analysis of existing data on human T-cell epitopes with confirmed processing deposited was performed using the Immune Epitope Database (IEDB, http://www.iedb.org/ (accessed on 25 February 2020)), as well as available literature data. The polyepitope cassettes were combined from S, N and/or M protein fragments enriched with selected T-cell epitopes. In order to optimize cellular processing of the cassettes, we carefully chose flanker regions of each epitope. The processing of resulting cassettes was assessed with IEDB tools to exclude undesirable neo-epitopes formation, and with allergen predicting tools (http://www.ddg-pharmfac.net/AllergenFP/ (accessed on 4 May 2020); http://www.allermatch.org/allermatch.py/form (accessed on 4 May 2020)) to exclude potential allergens.

Generation of Recombinant LAIV Viruses Expressing SARS-CoV-2 Polyepitope Cassettes
The cassettes were chemically synthesized by JSC BIOCAD (Saint Petersburg, Russia) and further cloned into NA or non-structural (NS) genes of H7N9 LAIV strain based on a master donor virus A/Leningrad/134/17/57 (H2N2) (Len/17), as previously described [22][23][24]. In brief, the cassettes were incorporated following the full-length N9 NA protein open reading frame (ORF), divided by a porcine teschovirus-1 (P2A) self-cleaving peptide [24,28]. In addition, the inserted cassette was followed by a duplicate fragment of NA gene, which is required for correct packaging. The insertion of the polyepitope cassettes into NS1 ORF, also separated by the P2A site, was accompanied by the truncation of the NS1 protein up to 126 residues. The polyepitope cassettes are supposed to be translated along with the NA or NS1 genes, followed by the cleavage of the two fragments via the P2A, which will facilitate independent intracellular processing of the inserted T-cell epitopes with proteasomes. The chimeric influenza virus genes were generated using Gibson cloning assembly method and inserted into pCIPolISapIT, a dual-promoter vector for reverse genetics of influenza virus. The XL-Gold E.coli cells were transformed with the resulting plasmids and then the plasmid DNAs were extracted using QIAGEN Plasmid Maxi Kit (QIAGEN, Hilden, Germany).
Recombinant influenza viruses carrying chimeric genes were rescued using the reverse genetics system developed for Len/17 master donor strain [29]. Briefly, 2 µg of each plasmid encoding HA and intact or chimeric NA gene of A/Anhui/1/2013 (H7N9) virus, an intact or chimeric NS gene and the remaining five genes of Len/17 virus were combined and transfected into Vero-WHO cells by electroporation using Neon Transfection System (Thermofisher Scientific, Waltham, MA, USA) with 100 µL Neon Kit according to manufacturer's protocol. After electroporation, cells were incubated for 6 h at 37 • C, 5% CO 2 in Opti-PRO serum-free medium supplemented with 1× GlutaMax (Gibco), followed by media change and the addition of 2.5 µg/mL trypsin (Sigma, Burlington, MA, USA). After 72 h incubation at 33 • C, 5% CO 2 , the cells were resuspended in culture medium and inoculated into 10-11-day old developing chicken embryos. After 72 h of incubation at 33 • C, the virus in chorioallantoic fluid was detected by hemagglutination test with 0.5% chicken red blood cells. Then, the rescued viruses were cloned by limiting dilutions in eggs, and the resulting clones were fully sequenced via Sanger sequencing. The selected clones with confirmed sequences were amplified in eggs and working stocks were prepared and stored at −70 • C for further analyses. Infectious titers of recombinant influenza viruses were determined in eggs and MDCK by end-point titration at 33 • C for 3 days and expressed as log 10 EID 50 /mL or log 10 TCID 50 /mL, respectively. We also assessed genetic stability of the rescued recombinant influenza viruses by performing ten serial passages in eggs, followed by Sanger sequencing of the chimeric viral genes.

Assessment of T-Cell Activation by Recombinant LAIV Viruses Using PBMCs of COVID-19 Convalescents
We used in vitro stimulation of PMBC specimens of COVID-19 convalescents with the chimeric LAIV viruses and subsequent intracellular cytokine staining (ICS) as indirect evidence for correct presentation of inserted SARS-CoV-2 T-cell epitopes delivered by the recombinant influenza viruses. For this purpose, peripheral blood mononuclear cells (PBMCs) were stimulated side-by-side with recombinant viruses, as well as with the LAIV vector, and the increased levels of cytokine-producing cells after stimulation with chimeric viruses relative to the vector control were interpreted as a positive response. Peripheral blood samples were collected from a cohort of blood donors who participated in the study of the persistence of SARS-CoV-2-specific T-cell responses after COVID-19 [27]. The study was approved by the local ethical committee of the Institute of Experimental Medicine (protocol #No 2/20 dated 7 April 2020), and all participants signed an informed consent. Twenty-five peripheral blood specimens were collected from twenty COVID-19 patients with disease onset from March 2020 until August 2020 and time post symptoms onset ranging from 1 to 5 months (Table S2). During this period, the Wuhan D614G SARS-CoV-2 variant dominated circulation in Russia, suggesting that study participants were infected with this strain [30]. None of the participants was vaccinated with LAIV for the preceding five years, although the history of natural influenza infections in prior years could not be ascertained. PBMCs were isolated by standard procedures using Lymphocyte Separation Medium (Corning, Corning, NY, USA) and resuspended in 2 mL of warm CR-0 medium (RPMI-1640 medium supplemented with 1× antibiotic/antimycotic, 10 mM HEPES (Gibco, Big Cabin, OK, USA), 50 µM β-mercaptoethanol, 20 U/mL roncoleukin). For ICS, 2 × 10 6 cells diluted in 50 µL of CR-0 medium were placed in a well of U-bottom plate and were stimulated by purified viruses in a dose of 3 MOI for 1 h at 37 • C under 5% CO 2 conditions. After 1 h, an FBS-containing medium was added to a final concentration of 10% FBS. The cells were incubated with virus for 16-18 h at 37 • C, 5% CO 2 . Non-stimulated cells were incubated in the same conditions without stimulation. To stop intracellular transport BD Golgi Plug (BD Biosciences, San Jose, CA, USA) was added to a final dilution 1:1000. For positive control, phorbol myristate acetate (PMA) and ionomycin mixture (PMA + ionomycin Cell Activation Cocktail, Biolegend, San Diego, CA, USA) was used according to the manufacturer's instructions. The cells were incubated for an additional 5 h at 37 • C, 5% CO 2 and then stained with Zombie Aqua Fixable Viability Kit (BioLegend, San Diego, CA, USA) and antibodies to surface antigens: CD4 (CD4-APC-Alexa Fluor*750, clone 13B8.2, Beckman Coulter, Brea, CA, USA), CD8 (CD8-PC5.5, clone B9.11, Beckman Coulter), CD3 (CD3-PC7, clone UCHT1, Beckman Coulter), CD45RA (CD45RA-ECD, clone 2H4, Beckman Coulter), CD197 (CCR7) (CD197-FITC clone 150503, BD Pharmingen, San Diego, CA, USA). After staining, cells were washed twice and permeabilized with fixation/permeabilization kit (BD Cytofix/Cytoperm) according to the manufacturer's manual. Cytokine staining was performed with PE Mouse-anti-human IFNγ antibody (clone B27, BD Pharmingen). After staining the cells were washed twice, fixed with Cytolast buffer (BioLegend, San Diego, CA, USA) and counted using Navios Flow Cytometer (Beckman Coulter). Data were analyzed with FlowJo software.

Immunization of HLA-A2.1 Transgenic Mice with LAIV/SARS-CoV-2 Vaccine Candidates
Groups of 11 eight-to twelve-week-old female HLA-A2.1 RANDOM TRANSGENIC mice (Taconic, Rensselaer, NY, USA) were inoculated intranasally (i.n.) with 10 6 EID 50 of each recombinant influenza virus and LAIV virus vector, in a volume of 50 µL, under isoflurane anesthesia. On day 3 p.i. four mice from each group were euthanized by an overdose of isoflurane, and nasal turbinates and lungs were collected for the assessment of viral replication in respiratory tract. Tissue homogenates were prepared in 1 mL PBS using a small bead mill (TissueLyser LT, QIAGEN, Hilden, Germany), centrifuged at 3500 rpm for 15 min, and viral titers in supernatants were determined by titration in eggs as described above. On day 21, the remaining mice were i.n. inoculated with the second dose of the same virus, and all animals were sacrificed ten days after revaccination. Blood samples were collected to study influenza virus-specific antibody responses in hemagglutination inhibition (HAI) assay and enzyme-linked immunosorbent assay (ELISA). In addition, splenocytes were isolated to study influenza-and SARS-CoV-2-specific cellular responses by ELISpot and ICS assays as specified below.

Immunization and Viral Challenge of Golden Syrian Hamsters
Groups of sixteen ten-to fourteen-weeks-old female Golden Syrian hamsters (Stolbovaya animal breeding nursery laboratory, Moscow region, Russia) were i.n. inoculated with 5 × 10 6 EID 50 of a recombinant LAIV/SARS-CoV-2 virus, as well as LAIV control virus, in a volume of 100 µL, under isoflurane anesthesia. On day 4 p.i., four hamsters were euthanized by an overdose of isoflurane to determine vaccine virus titers in the respiratory tract, as described above. The second vaccination was performed on day 21 of the study and three weeks after the second dose blood samples were collected from six animals in each group via the retro-orbital sinus, to determine influenza virus-specific antibody responses in ELISA. On day 45 of the study, immunized animals from each study group were divided into three groups of four hamsters which were infected i.n. with 100 µL of one of three viruses: (i) 10 6 EID 50 of Sh/PR8 influenza virus; (ii) 10 5 TCID 50 of SARS-CoV-2 (Wuhan lineage); and (iii) 10 5 TCID 50 of SARS-CoV-2 (Delta lineage). Influenza virusinfected animals were euthanized four days after infection, and respiratory tissues (NT and Vaccines 2022, 10, 1142 6 of 27 lungs) were collected and weighed for the assessment of virus loads. Tissue homogenates were titrated on MDCK cells and viral titers were expressed in log 10 TCID 50 /gram tissue. SARS-CoV-2-infected hamsters were monitored for weight loss and clinical symptoms (appearance, coat condition: 0-normal, 1-lack of grooming; interaction with other animals: 0-normal; 1-reduced; food consumption: 0-normal, 1-reduced; behavior on open area: 0-active, 1-reduced; response to pick up: 0-normal, 1-reduced) until day 5 p.i., when they were sacrificed for assessment of virological, immunological and pathological endpoints of protection. The lungs were perfused with 10 mL of PBS through the right ventricle and macroscopically assessed for the presence of pathological changes. One lung lobe was used for histopathological analyses, while the remaining tissue was weighed and homogenized to determine viral loads by TCID 50 assay in Vero-E6 cells. In addition, nasal turbinates were collected to determine SARS-CoV-2 viral titers in Vero-E6 cells. Hamster splenocytes isolated on day 5 post-SARS-CoV-2 challenge were used to assess cellular recall responses by IFNγ ELISpot assay, as described below.

Virus-Specific Antibody Immune Responses
Anti-influenza antibody levels were assessed in serum samples of immunized animals using HAI assay and/or ELISA, as described elsewhere [23]. Briefly, for HAI assay, serum samples were treated with receptor destroying enzyme (RDE) (Senka, Japan) and two-fold dilutions were incubated with four hemagglutination units of the H7N9 LAIV virus for 1 h at room temperature, followed by the addition of an equal volume of 0.5% chicken red blood cells. HAI titer was determined as the last serum dilution with complete inhibition of hemagglutination.
For influenza-specific ELISA, 50 ng/well of sucrose-purified LAIV virus were adsorbed on high-binding 96-well plates (Thermo, Waltham, MA, USA) overnight at +4 • C. Then, the wells were blocked with 1% of bovine serum albumin (BSA) in PBS, washed with PBS-Tween 20 (PBST), and two-fold serum dilutions were added to the wells and incubated for 1 h at 37 • C. After washing with PBST, an anti-mouse or anti-hamster IgG antibody conjugated to horseradish peroxidase (Sigma, Burlington, MA, USA) were added and the plates were incubated for another 1 h, followed by color development with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Waltham, MA, USA). The results were read on xMark Microplate spectrophotometer (BioRad, Hercules, CA, USA) at a wavelength of 450 nm. Antibody titer was calculated as the maximum serum dilution with an optical density at 450 nm (OD 450 ) value exceeding the control values at least two times.
SARS-CoV-2-specific ELISA was performed with sucrose gradient purified whole viral antigen (Wuhan and Delta) as described above for influenza virus, except that the SARS-CoV-2-coated plates were further inactivated by adding 2% buffered formalin and incubating the plates overnight at 4 • C, prior to treatment with BSA.

Assessment of Cellular Responses by ICS Assay
Splenic tissues were homogenized with a pestle, followed by filtering through a 70 µm cell strainer (Becton Dickinson, Franklin Lakes, NJ, USA). Red blood cells were lysed by an ammonium-chloride potassium lysing buffer (Thermo Fisher Scientific) and the single cell suspensions were maintained in CR-0 media. Purified murine splenocytes were placed in U-bottom 96-well plates, at a concentration 10 6 cells per well, in a volume of 50 µL. A sucrose gradient-purified LAIV virus was diluted in a CR-0 medium and 50 µL were added to the cells to reach a multiplicity of infection of 3. After incubation for 1 h at 37 • C and 5% CO 2 , 50 µL of CR-30 was added to achieve 10% final concentration of FBS. Non-stimulated cells were maintained under CR-10 medium. After 16-18 h incubation at 37 • C, 5% CO 2 , 50 µL of GolgiPlug solution at a 1:250 dilution (Becton Dickinson, Franklin Lakes, NJ, USA). At the same time, other wells were stimulated with SARS-CoV-2 peptide mix or NP 366 influenza peptide, each peptide was diluted to a concentration 1 µg per well. Positive control wells were stimulated with PMA-ionomycin mixture. The plates were incubated for another five hours, followed by staining with a panel of fluorescently labeled antibodies: ZombieAqua, CD4-PerCP/Cy5.5, CD8-APC/Cy7, CD62L-BV421, and CD44-PE (all from BioLegend, San Diego, CA, USA). After fixation and permeabilization, the cells were stained for intracellular cytokines with antibodies IFNγ-FITC, TNFα-APC and IL-2-PE/Cy7 (all from BioLegend, San Diego, CA, USA). After staining, the cells were washed, fixed with Cytolast buffer and counted using Navios Flow Cytometer. The data were analyzed with FlowJo software.

Assessment of Cellular Responses by ELISpot Assay
Splenocytes isolated from mice and Syrian hamsters were processed identically, except that different ELISpot kits were used to detect IFNγ production: Mouse IFN-γ Single-Color ELISPOT (Cellular Technology Ltd., Shaker Heights, OH, USA) and Hamster IFN-γ ELISpot Plus kit (Mabtech, Nacka Strand, Sweden) using the instructions of the manufacturers. Briefly, pre-coated with anti-IFNγ antibody ELISpot plates were washed 4 times with sterile PBS and then incubated with CR-10 media for 30 min at room temperature. A separate sterile 96-well plate was used to mix splenocytes with one of the desired stimuli: either purified influenza virus at an MOI 1.0, or purified SARS-CoV-2 virus at an MOI 0.1, or PepTivator S + N mixture (30 pmol per peptide), or specific peptides (1 µg per well). Then the media was removed from the ELISpot plate and the mixtures were added to each well, followed by 18 h incubation at 37 • C, 5% CO 2 . The detection of spots was performed according to the manufacturer's protocol using detection antibody and ready-to use substrate solution. Color development was stopped by extensively washing in tap water and then the plates were left to dry overnight. Spots were counted in an AID vSpot Spectrum reader (Advanced Imaging Devices, Strassberg, Germany).

Statistical Analysis
Data were analyzed using Graph Pad Prism 6 software. Statistically significant differences between several study groups were determined by one-way or two-way ANOVA with Tukey's multiple comparison test. Differences between the levels of IFNγ-secreting T cells between LAIV vector and LAIV/SARS-CoV-2 recombinant viruses were assessed by non-parametric analysis using the Wilcoxon T-test. Statistical significance of the difference between two groups (evaluation of viral titers in vitro and in respiratory tracts) was determined by the Mann-Whitney U-test. p values of <0.05 were considered significant.

Selection of SARS-CoV-2 Fragments Enriched with Conserved T-Cell Epitopes and Generation of Recombinant LAIV/SARS-CoV-2 Viruses
Structural proteins of SARS-CoV-2 (S, N, M) have been reported to be the main targets for T-cell response, as well as proteins synthesized early in the viral lifecycle [10,[34][35][36][37][38]. Non-structural proteins of SARS and SARS-2 have high rate of conservancy, however, there are several warnings against using conservative parts of replicase, proteinase or other proteins in vaccine development. In studies with murine coronaviruses some replicase epitopes induced autoimmune neurological disorders because of its homology with myelin proteolipid protein [39,40].
This study was initiated at the beginning of COVID-19 pandemic, and the initial selection of fragments was based on sequence conservation between SARS-CoV and SARS-CoV-2 and data about experimentally confirmed epitopes deposited in IEDB and reported in literature. We were focused on epitopes with confirmed processing and presentation, this allows us to develop a method of assessment of epitope processing in a "model" situation. The main restriction of T-cell assays is poor allele coverage: the majority of well-studied epitopes are HLA-A*02:01-restricted because of availability of corresponding animal models. For SARS, large-scale analysis of binding of SARS peptides to human MHC was performed by different laboratories which deposited these data directly in IEDB [41][42][43][44][45]. It is supposed that a successful strategy of a T cell-based vaccine design is to Vaccines 2022, 10, 1142 9 of 27 combine experimentally confirmed T-cell epitopes with peptides with established MHC binding, in order to cover the majority of the human population [46].
Since the capacity of an influenza virus genome for the insertion of foreign transgenes is rather limited, we designed several variants of inserts encoding polyepitope cassettes ranging from~100 to~170 residues and comprising of different fragments of S, N and M proteins which contain a number of conserved epitopes with confirmed processing and immunogenicity, as well as peptides that were reported to form complexes with different human MHC molecules ( Figure 1). More detailed information on the experimentally confirmed epitopes included in each selected fragment is provided in Supplementary (Figures S1-S8, Tables S3-S10). The cassettes were synthesized de novo and inserted into NA or NS1 genes of H7N9 LAIV virus, as shown in Figure 2. The insertion of the P2A site upstream of the transgene was supposed to facilitate the independent intracellular processing of SARS-CoV-2 epitopes ( Figure 2). In this study, eleven rescued LAIV/SARS-CoV-2 variants have been evaluated in detail, five of which had the insertion into NA gene, while the other six variants had NS gene modified ( Table 1). All recombinant LAIV viruses were genetically stable after ten passages in eggs, with no undesired mutations detected within the chimeric influenza genes.

Replicative Properties of Recombinant LAIV Viruses In Vitro and In Vivo
All recombinant viruses, as well as the control H7N9 LAIV strain, were grow eggs and their titers in this substrate served as the main indicative of the impact o inserted foreign fragments on viral replicative properties. Strikingly, only one out chimeric viruses (FluCoVac-28) had very similar titer to the LAIV vector, while the variants showed the titer reduction from 1.4 to 3.0 log10EID50 ( Table 1). The greatest im was noted when the SARS-CoV-2 cassettes were inserted into the NS gene of the influ virus, whereas modification of NA gene had a less pronounced effect on viral grow eggs. Importantly, there was no correlation between the size of the inserted foreign fragment and the activity of viral growth in eggs. Similar results were obtained vi titration of recombinant LAIV viruses on MDCK cells: all chimeric viruses with the m ified NS gene were not able to be replicated in this substrate, most likely due to the e of NS1 truncation and not the insertion of the transgenes themselves.
Groups of four HLA-A2.1 transgenic mice were inoculated i.n. with the chimer

Replicative Properties of Recombinant LAIV Viruses In Vitro and In Vivo
All recombinant viruses, as well as the control H7N9 LAIV strain, were grown in eggs and their titers in this substrate served as the main indicative of the impact of the inserted foreign fragments on viral replicative properties. Strikingly, only one out of 11 chimeric viruses (FluCoVac-28) had very similar titer to the LAIV vector, while the other variants showed the titer reduction from 1.4 to 3.0 log 10 EID 50 ( Table 1). The greatest impact was noted when the SARS-CoV-2 cassettes were inserted into the NS gene of the influenza virus, whereas modification of NA gene had a less pronounced effect on viral growth in eggs. Importantly, there was no correlation between the size of the inserted foreign gene fragment and the activity of viral growth in eggs. Similar results were obtained via the titration of recombinant LAIV viruses on MDCK cells: all chimeric viruses with the modified NS gene were not able to be replicated in this substrate, most likely due to the effect of NS1 truncation and not the insertion of the transgenes themselves.
Groups of four HLA-A2.1 transgenic mice were inoculated i.n. with the chimeric viruses at a dose of 10 6 EID 50 to assess their replicative properties in the upper and lower respiratory tract. On day 3 post inoculation, no live virus or only some residual quantities could be detected in the mouse lungs, suggesting the attenuated phenotype of all variants ( Table 1). All vaccine candidates with modified NA gene replicated in nasal turbinates of mice at the level of classical LAIV virus which indicated the absence of a negative effect of such LAIV genome modification. In contrast, all viruses with the modified NS1 gene lacked active viral replication in the upper respiratory tract (Table 1), which was in line with previously published findings on the reduced replication of influenza viruses expressing truncated NS1 proteins in a mouse model [47,48].

Immunogenicity of the Recombinant LAIV-SARS-CoV-2 Viruses in Transgenic Mice
Since the inserted SARS-CoV-2 cassettes contained a number of confirmed HLA-A2 restricted CTL epitopes, we used HLA-A2.1 transgenic mice to evaluate humoral and cell-mediated immunity upon intranasal immunization with the recombinant LAIV/SARS-CoV-2 viruses. The chimeric viruses were tested in two independent experiments: the first study assessed six vaccine prototypes carrying cassettes #1 to #6, while the second experiment involved five variants expressing cassettes #7 to #10. Groups of 7 mice received two doses of the vaccines, 10 6 EID 50 each, with an interval of 21 days. On day 10 postsecond dose, mice were sacrificed, and serum samples were used for assessment of antibody immune responses, while spleens were collected to study cell-mediated immunity.

Influenza-Specific Antibody Immune Responses
Serum samples were assessed by influenza virus-specific HAI and ELISA assays. Despite the lack of detectable virus replication in some vaccine prototypes with modified NS genes, all vaccine candidates induced a virus-specific antibody, though the magnitude of these responses varied significantly between groups ( Figure 3). These data suggest that the particular sequence of the inserted SARS-CoV-2 cassette can have an influence on the immunogenicity of the influenza virus vector itself. For example, viruses FluCoVac-13 and FluCoVac-14 have an identical size of the cassettes, which were inserted into the NS1 gene, but the antibody responses of the FluCoVac-13 prototype were 5-6 times higher than that of FluCoVac-14 strain (Figure 3). Another important observation is that the site of cassette insertion had no impact on the immunogenicity of the LAIV virus, since FluCoVac-27 and FluCoVac-32 candidates induced comparable influenza-specific antibody responses despite the differences in the replicative activity of the viruses in the mouse upper respiratory tract (Table 1). From the other side, the insertion of the shorter cassette into the NS gene could lead to a lower immunogenicity than the longer insert, as was evidenced by the pair of viruses FluCoVac-29 and FluCoVac-31 (Figures 1 and 3). Vaccines 2022, 10, x FOR PEER REVIEW 13 of 28

Influenza-and SARS-CoV-2-Specific T-Cell Responses
Isolated mouse splenocytes were stimulated with a sucrose-purified LAIV virus to assess influenza-specific cellular responses. Both IFNγ ELISpot assay and flow cytometry analysis confirmed the induction of robust T-cell responses to influenza virus, with some variations in the magnitude of the response, which generally correlated with influenzaspecific antibody responses. In particular, the least immunogenic candidates FluCoVac-14 and FluCoVac-29 demonstrated the lowest levels of activated IFNγ-secreting T cells in both assays (Figure 4).
Strikingly, stimulation of the murine splenocytes with a mixture of SARS-CoV-2 peptides which corresponded to the inserted HLA-A*02:01 CD8 T-cell epitopes did not result in significant IFNγ production ( Figure 4). We confirmed the expression of the HLA-A2 molecules on the mouse splenic cells using the PE Anti-HLA A2 antibody [BB7.2] (Abcam) ( Figure S9), as well as the development of significant response to CEF control peptides, which contain immunodominant influenza M1 (GILGFVFTL) and PA (FMYSDFHFI) HLA-A2-restricted CTL epitopes ( Figure S10). These data might indicate that the HLA-A2-restricted epitopes could be correctly processed and presented in this animal model; however, the immunodominance of influenza-specific epitopes can interfere with the response to the epitopes of interest. Moreover, T-cell responses to the mouse H2-Db-restricted influenza epitopes could also affect the immunogenicity of the inserted HLA-A2restricted SARS-CoV-2 epitopes. Indeed, the HLA-A2.1 random transgenic mice originate from C57BL/6J background, which is known to generate a dominant CTL response to

Influenza-and SARS-CoV-2-Specific T-Cell Responses
Isolated mouse splenocytes were stimulated with a sucrose-purified LAIV virus to assess influenza-specific cellular responses. Both IFNγ ELISpot assay and flow cytometry analysis confirmed the induction of robust T-cell responses to influenza virus, with some variations in the magnitude of the response, which generally correlated with influenzaspecific antibody responses. In particular, the least immunogenic candidates FluCoVac-14 and FluCoVac-29 demonstrated the lowest levels of activated IFNγ-secreting T cells in both assays ( Figure 4).
Strikingly, stimulation of the murine splenocytes with a mixture of SARS-CoV-2 peptides which corresponded to the inserted HLA-A*02:01 CD8 T-cell epitopes did not result in significant IFNγ production ( Figure 4). We confirmed the expression of the HLA-A2 molecules on the mouse splenic cells using the PE Anti-HLA A2 antibody [BB7.2] (Abcam) (Figure S9), as well as the development of significant response to CEF control peptides, which contain immunodominant influenza M1 (GILGFVFTL) and PA (FMYS-DFHFI) HLA-A2-restricted CTL epitopes ( Figure S10). These data might indicate that the HLA-A2-restricted epitopes could be correctly processed and presented in this animal model; however, the immunodominance of influenza-specific epitopes can interfere with the response to the epitopes of interest. Moreover, T-cell responses to the mouse H2-Db-restricted influenza epitopes could also affect the immunogenicity of the inserted HLA-A2-restricted SARS-CoV-2 epitopes. Indeed, the HLA-A2.1 random transgenic mice originate from C57BL/6J background, which is known to generate a dominant CTL response to NP 366 epitope of influenza virus [49,50]. In fact, the stimulation of splenic cells of immunized mice with the NP 366 peptide revealed robust epitope-specific responses in all test groups ( Figure S11). This study highlighted the importance of the development of relevant animal models for the assessment of human epitope-specific T-cell responses to the epitope-based vaccine prototypes against various diseases.  [49,50]. In fact, the stimulation of splenic cells of immunized mice with the NP366 peptide revealed robust epitope-specific responses in all test groups ( Figure S11). This study highlighted the importance of the development of relevant animal models for the assessment of human epitope-specific T-cell responses to the epitope-based vaccine prototypes against various diseases.

Assessment of Recombinant LAIV Viruses in ICS Assay with COVID-19 Convalescents Samples
We stimulated PMBCs isolated from COVID-19 recovered patients with the chimeric LAIV/SARS-CoV-2 viruses as a surrogate assay for the detection of correct processing and presentation of the inserted SARS-CoV-2 T-cell epitopes. Similar to mouse studies, the viruses were tested in two independent experiments with six and five variants carrying cassettes #1-6 and #7-10, respectively. A side-by-side stimulation of PBMCs with recombinant viruses and the LAIV vector revealed significantly higher levels of IFNγ-producing effector memory CD4 T cells in almost all tested vaccine candidates, relative to the LAIV control ( Figure 5A). It should be noted that the levels of CD8 TEM cells activated by the chimeric viruses significantly varied between blood donors ( Figure 5B), with a trend of reducing the IFNγ-positive proportion with the increase of the time post symptom onset, which can be explained by a rapid decline of the SARS-CoV-2 specific CD8 TEM within several months after recovery, whereas the CD4 TEM subset can be maintained for a longer period [27]. The use of PBMCs of COVID-19 naïve individuals didn't reveal significant differences in cytokine-producing CD4 and CD8 T cells between recombinant viruses and

Assessment of Recombinant LAIV Viruses in ICS Assay with COVID-19 Convalescents Samples
We stimulated PMBCs isolated from COVID-19 recovered patients with the chimeric LAIV/SARS-CoV-2 viruses as a surrogate assay for the detection of correct processing and presentation of the inserted SARS-CoV-2 T-cell epitopes. Similar to mouse studies, the viruses were tested in two independent experiments with six and five variants carrying cassettes #1-6 and #7-10, respectively. A side-by-side stimulation of PBMCs with recombinant viruses and the LAIV vector revealed significantly higher levels of IFNγ-producing effector memory CD4 T cells in almost all tested vaccine candidates, relative to the LAIV control ( Figure 5A). It should be noted that the levels of CD8 T EM cells activated by the chimeric viruses significantly varied between blood donors ( Figure 5B), with a trend of reducing the IFNγ-positive proportion with the increase of the time post symptom onset, which can be explained by a rapid decline of the SARS-CoV-2 specific CD8 T EM within several months after recovery, whereas the CD4 T EM subset can be maintained for a longer period [27]. The use of PBMCs of COVID-19 naïve individuals didn't reveal significant differences in cytokine-producing CD4 and CD8 T cells between recombinant viruses and the LAIV control ( Figure S12). Overall, the results of the ICS analysis suggest that the SARS-CoV-2 T-cell epitopes delivered by LAIV virus vector can be processed by the human immune system with a potential of inducing protective T-cell responses.
the LAIV control ( Figure S12). Overall, the results of the ICS analysis suggest that the SARS-CoV-2 T-cell epitopes delivered by LAIV virus vector can be processed by the human immune system with a potential of inducing protective T-cell responses.

Immunogenicity and Protective Activity of a Selected LAIV/SARS-CoV-2 Vaccine Prototype in Syrian Hamsters
Based on in vitro and in vivo characterization of the developed recombinant LAIV/SARS-CoV-2 variants, we selected FluCoVac-28 candidate for its further characterization in a Syrian hamster model, because this virus replicated well both in eggs and MDCK cells (Table 1), induced influenza-specific immunity in mice at the level of classical LAIV variant (Figures 3 and 4), and due to the potent activation of effector memory cytotoxic and helper T cells in in vitro tests on PBMCs of COVID-19 convalescents ( Figure 5).
Groups of sixteen Golden Syrian hamsters were i.n. immunized with two doses of 5 × 10 6 EID50 of the FluCoVac-28 and LAIV viruses, 3 weeks apart, while control animals received placebo (PBS). Both influenza viruses replicated to the high titers in the hamster nasal turbinates on day 3 p.i., while no replication was observed in the lung tissues, which confirmed the attenuated phenotype of the vaccine variants ( Figure 6A). Serum samples collected from eight animals in each group on day 21 post second dose were assessed for

Immunogenicity and Protective Activity of a Selected LAIV/SARS-CoV-2 Vaccine Prototype in Syrian Hamsters
Based on in vitro and in vivo characterization of the developed recombinant LAIV/SARS-CoV-2 variants, we selected FluCoVac-28 candidate for its further characterization in a Syrian hamster model, because this virus replicated well both in eggs and MDCK cells (Table 1), induced influenza-specific immunity in mice at the level of classical LAIV variant (Figures 3 and 4), and due to the potent activation of effector memory cytotoxic and helper T cells in in vitro tests on PBMCs of COVID-19 convalescents ( Figure 5).
Groups of sixteen Golden Syrian hamsters were i.n. immunized with two doses of 5 × 10 6 EID 50 of the FluCoVac-28 and LAIV viruses, 3 weeks apart, while control animals received placebo (PBS). Both influenza viruses replicated to the high titers in the hamster nasal turbinates on day 3 p.i., while no replication was observed in the lung tissues, which confirmed the attenuated phenotype of the vaccine variants ( Figure 6A). Serum samples collected from eight animals in each group on day 21 post second dose were assessed for the presence of influenza and SARS-CoV-2 whole virus-specific IgG antibody in ELISA, while four hamsters in each group were infected intranasally with 10 6 EID 50 of a homologous H7N9 PR8-based influenza virus, followed by tissue collection on day 4 post challenge for virus titration. As expected, both viruses induced robust influenza-specific immune responses ( Figure 6B) and protected animals against challenge with a homologous influenza virus infection ( Figure 6C). the presence of influenza and SARS-CoV-2 whole virus-specific IgG antibody in ELISA, while four hamsters in each group were infected intranasally with 10 6 EID50 of a homologous H7N9 PR8-based influenza virus, followed by tissue collection on day 4 post challenge for virus titration. As expected, both viruses induced robust influenza-specific immune responses ( Figure 6B) and protected animals against challenge with a homologous influenza virus infection ( Figure 6C). Importantly, no SARS-CoV-2-specific serum IgG antibody responses were noted in immunized hamsters, since the T-cell cassette did not include immunodominant B-cell epitopes ( Figure 7A). Immunized hamsters were also challenged with 10 5 TCID50 of Wuhan and Delta variants of SARS-CoV-2 on day 21 after second immunization (four animals per challenge). Hamsters immunized with FluCoVac-28 prototype were protected against weight loss and clinical manifestation of the disease if compared to the PBS and LAIV groups after challenge with Wuhan variant (Figure 7B,C). Interestingly, body weight loss was reduced in both LAIV groups when the animals were infected with Delta virus (Figure 7B). Clinical symptoms of the Delta variant were not pronounced in the control PBS group, and no significant differences were observed by this parameter between the test vaccines ( Figure 7C). Interestingly, significant reduction of viral titers in the FluCoVac-28 immunized hamsters was detected only in the nasal turbinates of animals challenged with both SARS-CoV-2 variants ( Figure 7D). Although a three-fold reduction was noted in the mean viral pulmonary titers in this test group relative to the mock-immunized animals, these differences were not statistically significant, probably due to the low number of animals in each group (n = 4). Importantly, no SARS-CoV-2-specific serum IgG antibody responses were noted in immunized hamsters, since the T-cell cassette did not include immunodominant B-cell epitopes ( Figure 7A). Immunized hamsters were also challenged with 10 5 TCID 50 of Wuhan and Delta variants of SARS-CoV-2 on day 21 after second immunization (four animals per challenge). Hamsters immunized with FluCoVac-28 prototype were protected against weight loss and clinical manifestation of the disease if compared to the PBS and LAIV groups after challenge with Wuhan variant (Figure 7B,C). Interestingly, body weight loss was reduced in both LAIV groups when the animals were infected with Delta virus ( Figure 7B). Clinical symptoms of the Delta variant were not pronounced in the control PBS group, and no significant differences were observed by this parameter between the test vaccines ( Figure 7C). Interestingly, significant reduction of viral titers in the FluCoVac-28 immunized hamsters was detected only in the nasal turbinates of animals challenged with both SARS-CoV-2 variants ( Figure 7D). Although a three-fold reduction was noted in the mean viral pulmonary titers in this test group relative to the mock-immunized animals, these differences were not statistically significant, probably due to the low number of animals in each group (n = 4).  The protective effect of vaccination was further studied by histopathological assessment of SARS-CoV-2-induced lung pathology. After challenge with Delta variant, inflammatory lesions in the lungs of mock-immunized hamsters were located predominantly around large bronchi and vessels, characterized by extensive involvement of the lung parenchyma, pronounced bronchiolitis/bronchitis and endotheliitis (Figures 8A and S14). The bronchiolar epithelium was partially necrotic and desquamated into the lumen of the large bronchi. Along with necrobiotic changes, foci of regenerative hyperplasia of the respiratory epithelium were determined. Peribronchial and perivasal lymphohistiocytic infiltrates spread over a vast area of the lung parenchyma, lysing the walls of the alveoli and respiratory bronchioles. On the periphery, thickening and edema of the interalveolar septa, hyperplasia of type II alveolocytes were determined. The lumen of the alveoli contained a mixture of cell debris, polymorphocellular leukocytes, necrotic alveolocytes, fibrin and erythrocytes ( Figures 8A and S14). Interestingly, lung pathology of the LAIV-immunized hamsters was less pronounced compared to the control animals. In particular, inflammatory infiltration was located focally around several large bronchioles, characterized by a moderate amount of involvement of the lung parenchyma. The spread of inflammatory infiltration was segmental, while bronchioles and large vessels were affected in the apical segments only. Minor peribronchial infiltrations and foci of thickening of the interalveolar septa with lymphocytic infiltration were determined, which were sporadic ( Figure 8). Nevertheless, lungs of the hamsters immunized with the recombinant LAIV/SARS-CoV-2 virus were the least affected, as their histoarchitectonics was comparable to the intact animals. Single foci of bronchiolitis and inflammatory infiltration of a moderate volume, as well as an insignificant volume of interalveolar septa thickened due to lymphoplasmacytic infiltration, were found in some lung lobes (Figures 8A and S14). A semi-quantitative analysis of lung pathology revealed significant reduction in scoring values of airway, lung/alveolar and vascular damage in the FluCoVac-28 group compared to the mock-immunized animals ( Figure 8B). Interestingly, the airway and vascular scores were also reduced in the LAIV-immunized hamsters compared to the PBS group ( Figure 8B), which correlated with the reduced weight loss ( Figure 7B), suggesting some degree of cross-protectivity afforded by the classical LAIV virus against SARS-CoV-2 infection. Noteworthy, this cross-protection was not observed when the immunized hamsters were challenged with SARS-CoV-2 Wuhan variant: both mock-and LAIV-immunized hamsters had severe lung lesions on day 5 after challenge, in form of broncho-interstitial pneumonia characterized by diffuse alveolar damage (Figures S13 and S15). These differences can be explained by the varying degree of pathogenicity of different SARS-CoV-2 variants in this animal model [51].
To find possible mechanism of protective effect of the chimeric LAIV/SARS-CoV-2 virus against SARS-CoV-2, we measured the levels of virus-specific cell-mediated responses by stimulating hamster splenocytes collected five days post challenge with live SARS-CoV-2 or PepTivator, followed by counting IFNγ-secreting cells by ELISpot. Interestingly, in animals from the naïve group significant numbers of spots were detected in both challenge experiments (up to 100 and 200 spots per 500,000 cells for Delta and Wuhan challenge, respectively) (Figure 9), suggesting that the immune system has been activated by day 5 after infection. Nevertheless, the FluCoVac-28-immunized hamsters demonstrated approximately three-fold higher levels of SARS-CoV-2-specific cells, which indicated reactivation of memory T-cell responses established by primary vaccination (Figure 9). Of note, the LAIV-immunized hamsters also demonstrated increased IFNγ production after challenge with SARS-CoV-2, most probably due to the nonspecific activation of SARS-CoV-2-specific T cells by the presence of robust T-cell immunity to influenza after LAIV immunization. To find possible mechanism of protective effect of the chimeric LAIV/SARS-CoV-2 virus against SARS-CoV-2, we measured the levels of virus-specific cell-mediated responses by stimulating hamster splenocytes collected five days post challenge with live SARS-CoV-2 or PepTivator, followed by counting IFNγ-secreting cells by ELISpot. Interestingly, in animals from the naïve group significant numbers of spots were detected in both challenge experiments (up to 100 and 200 spots per 500,000 cells for Delta and Wuhan challenge, respectively) (Figure 9), suggesting that the immune system has been activated by day 5 after infection. Nevertheless, the FluCoVac-28-immunized hamsters demonstrated approximately three-fold higher levels of SARS-CoV-2-specific cells, which indicated reactivation of memory T-cell responses established by primary vaccination ( Figure  9). Of note, the LAIV-immunized hamsters also demonstrated increased IFNγ production after challenge with SARS-CoV-2, most probably due to the nonspecific activation of SARS-CoV-2-specific T cells by the presence of robust T-cell immunity to influenza after LAIV immunization.

Discussion
Despite the successful implementation of first generation COVID-19 vaccines to reduce the pandemic burden, intensive research continues in an attempt to design vaccine candidates with improved characteristics. Such improved vaccines should have broader reactivity for protection against emerging coronaviruses and, if possible, combine protection against several most important respiratory pathogens. Due to the proven ability of SARS-CoV-2 to evolve antigenically and escape neutralizing antibody immunity raised to the prior infection or vaccination with Spike-based vaccines, the development of vaccines targeting to the mutationally constrained T-cell epitopes is considered highly promising [52]. Furthermore, there is evidence that soon after infection with SARS-CoV-2, especially

Discussion
Despite the successful implementation of first generation COVID-19 vaccines to reduce the pandemic burden, intensive research continues in an attempt to design vaccine candidates with improved characteristics. Such improved vaccines should have broader reactivity for protection against emerging coronaviruses and, if possible, combine protection against several most important respiratory pathogens. Due to the proven ability of SARS-CoV-2 to evolve antigenically and escape neutralizing antibody immunity raised to the prior infection or vaccination with Spike-based vaccines, the development of vaccines targeting to the mutationally constrained T-cell epitopes is considered highly promising [52]. Furthermore, there is evidence that soon after infection with SARS-CoV-2, especially the mild form, people do not have detectable virus-specific antibodies, although T-cell immunity is quite robust, either because no antibodies have been produced or they were very short-lived, whereas T-cell memory lasts much longer [53]. Indeed, analysis of blood samples taken from survivors of the previous coronavirus outbreak caused by SARS-CoV found that the virus-specific memory T cells can persist as long as 11 years post-infection [54], suggesting that the T cell-based COVID-19 vaccines could induce durable protection.
In terms of vaccine development, the most promising studies for T-cell targeted vaccine are T-cell studies, which confirm not only the ability of peptide to bind to MHC molecule, but also to induce T-cells response, cytotoxicity, provide protection and form a population of memory T cells. However, due to the limited accessibility of HLA-transgenic animals, the immunogenic HLA-A*02:01-restricted epitopes are presented in IEDB database in a disproportionately greater number than the epitopes restricted by other alleles, which are predominantly characterized by the ability to bind to the corresponding human MHCs, but not by the immunogenic properties [41,44,45]. In vaccine design, the best strategy seems to be to combine experimentally confirmed T-cell epitopes with peptides with established MHC-binding to cover all human population. Therefore, in the current study we designed several cassettes comprising of various conserved SARS-CoV-2 regions enriched with the T-cell epitopes, which were either assembled from short parts of S, M, and N proteins carrying immunogenic CTL epitopes, or contained more prolonged SARS-CoV-2 fragments that included both cytotoxic and helper T-cell epitopes. In this cassette design we kept in mind that the order of epitopes and specific flanking regions can significantly affect epitope processing [62,63]. Furthermore, possible presence of epitopes with allergic properties was closely monitored to avoid undesired immune reactions to vaccination.
A limitation of the study is that some immunodominant and conserved SARS-CoV-2 T-cell epitopes were not included into our T-cell cassettes as they were discovered after the cassette design was completed and further optimization on the composition of T-cell epitopes within the recombinant influenza viruses should be continued to update epitope content in the T-cell cassettes. Nevertheless, the main advantage of the T cell-based vaccines is that the target epitopes can be combined into relatively short multiepitope cassettes that can be delivered even by viral vectors with low genome capacity, such as influenza virus.
In this study, we chose LAIV as a delivery vehicle as it was previously shown to be effective inducer of T-cell immunity in humans [64,65] and because our previous studies demonstrated the possibility of constructing LAIV-based viral vectored vaccines that efficiently induced T-cell responses to the inserted epitopes in mice [22,23,25]. In particular, vaccine prototypes developed in this study were based on potentially pandemic H7N9 LAIV strain as a viral vector, which was proven to be safe and immunogenic in humans [66]. Additional advantage is that the vaccine can be more effective in humans due to the lack of pre-existing immunity. Nevertheless, development of such vaccines based on seasonal LAIV viruses is also of high priority as the vaccine can be used as a bivalent vaccine for combined prophylaxis of the two most important respiratory pathogens, if the SARS-CoV-2 acquire similar seasonality as influenza.
We probed two strategies to generate a panel of recombinant LAIV viruses, with modification either NA or NS gene of the influenza virus. Both strategies have been successful in induction robust T-cell immunity to the inserted immunodominant epitopes in BALB/c [23] and C57BL/6J mice [22]. The NA modification doesn't impact the influenza protein itself, whereas NS1 protein was truncated to 126 residues in an attempt to increase vaccine's immunogenicity, as was demonstrated with other influenza strains [67,68]. However, in our study there was no significant enhancement of influenza-specific immune responses in NS-modified recombinant viruses compared to NA-modified. Moreover, the lack of detectable viral replication of the NS-modified strains interfered with robust antibody and cellular immunity, suggesting that truncation of the NS1 protein of a cold-adapted influenza virus makes it overattenuated and leads to the reduced immunogenicity. Nevertheless, the most important observation from the mouse study is that the particular content of the inserted foreign cassette had the most prominent effect on the influenza-specific immune responses than the size of the insert and the influenza virus gene modified. Although we did not evaluate the anti-influenza protective effect of recombinant LAIV/SARS-CoV-2 in mice in the current study because of the limited number of available animals, our previous results assume that NA-and NS-modified recombinant LAIVs can induce sterile immunity to influenza [22,23].
The lack of adequate small animal models to assess immunogenicity of human T-cell epitopes makes the development of COVID-19 T cell-based vaccines very challenging, resembling the hurdles researchers have encountered in testing epitope-based universal influenza vaccines [69], as the available transgenic animals usually express only one MHC allele, which may not reflect the real diversity of the human population. Since our constructs included confirmed HLA-A*02:01 CD8 T-cell epitopes, we studied immunogenicity of the recombinant LAIV/SARS-CoV-2 viruses on HLA-A2.1 transgenic mice, however, no significant SARS-CoV-2-specific responses were observed after intranasal immunization, most probably due to the immunodominance of influenza-specific mouse T-cell epitopes. For example, a side-by-side screening of T-cell epitopes in HLA-A2.1 transgenic mice and HLA-A*02:01-positive subjects who received a vaccine against vaccinia virus found that only 46% epitopes were detected in both systems, and the responses in transgenic mice against epitopes originally identified in mice were much stronger than for epitopes originally detected in humans, and vice versa [70].
Since the processing of T-cell cassettes containing SARS-CoV-2 epitopes in the influenza virus was not indicative in the humanized mice, we used an indirect in vitro test using PBMCs of people who recently recovered from COVID-19 to confirm processing of the inserted SARS-CoV-2 epitopes. It should be noted that all study participants had a history of exposure to influenza antigen, and some had received inactivated vaccine during their lifetime, but none had been immunized with LAIV. In this study, we compared the response to the LAIV antigen alone (i.e., the viral vector) with the response to recombinant LAIV viruses containing SARS-CoV-2 inserts and considered the differences in response to the two stimulating agents as a response to the inserted SARS-CoV-2 epitopes. In our study, the LAIV vector was based on the H7N9 influenza virus, which does not circulate in humans, so memory T-cell responses to HA and NA epitopes in our participants were not expected to be significant. In addition, LAIV was based on the Leningrad/17 H2N2 virus, and H2N2 viruses have not circulated in humans since 1968. All of our participants were born after 1968, so T-cell responses to internal proteins were also expected to be low.
In vitro stimulation of PBMC samples with most of tested live recombinant LAIV/SARS-CoV-2 viruses resulted in significant activation of cytotoxic and helper memory T cells in the group of COVID-19 convalescents, but not in the naïve subjects. These data indicate that the recombinant influenza viruses indeed can successfully express the inserted epitopes in infected cells, which are then presented by MHC-I and MHC-II molecules. The diverse levels of activated memory T cells in PBMC samples of COVID-19 convalescents can be explained by the varying time post symptoms onset, as these levels decrease over time [27] and also probably by the differences in the HLA alleles in our study participants.
Based on in vitro and in vivo characterization, we selected a promising vaccine prototype for further evaluation in pre-clinical studies. This candidate, FluCoVac-28, had a high ability to grow in both eggs and MDCK cells, induced robust influenza-specific humoral and cellular responses and was able to activate memory T cells in PBMCs of COVID-19 recovered patients. Moreover, the inserted T-cassette contained rather extended fragments of coronavirus proteins enriched with T-cell epitopes of various HLA restriction, including such immunogenic epitopes as RLQSLQTYV [58,71,72], RLDKVEAEV [72,73], GMEVTPSGTWLTYTGAIKLD [36,74], and others.
This selected prototype was further studied in Golden Syrian hamsters. Replicative properties of the recombinant virus and its ability to induce influenza-specific immunity were identical to the unmodified LAIV virus. Furthermore, the FluCoVac-28 protected immunized hamsters against both influenza and SARS-CoV-2 infections, which was confirmed by reduced weight loss, milder clinical symptoms and less pronounced histopathological sighs of SARS-CoV-2 infection in the lungs, compared to LAIV-and mock-immunized animals. Interestingly, although the recombinant virus was not effective in significant reduction of viral loads in the lungs of challenged Syrian hamsters, the animals were protected against lung immunopathology. These data are in line with other studies which found that virus-neutralizing antibodies are more effective in virus control than the T cells, but CD4 and CD8 T cells can effectively protect hamsters against antibody-resistant SARS-CoV-2 variants, without inducing lung immunopathology [75].
Although the data on hamster T-cell epitopes of SARS-CoV-2 is still limited, the induction of SARS-CoV-2-specific cellular responses and the observed protective effect against two antigenically diverse coronaviruses suggest that the inserted prolonged fragments of S and N proteins include a class I-and/or class II-restricted T cell subpopulations specific for this animal model. Interestingly, we observed some degree of non-specific protection afforded by classical LAIV virus against SARS-CoV-2 Delta variant. This phenomenon can be explained by the existence of cross-reactive epitopes in influenza virus and SARS-CoV-2 which can bind to the same T-cell receptors even in the absence of 100% sequence homology [76]. Furthermore, there is evidence that pre-existing T-cell immunity to influenza in healthcare workers correlated with cellular responses to SARS-CoV-2 after COVID-19 [77], suggesting that influenza immunity can contribute to the responses to SARS-CoV-2 infection and vaccination. Interestingly, similar results of nonspecific protection were observed in a study of another LAIV-based SARS-CoV-2 vaccine in Syrian hamsters [78]. The different level of non-specific protection of LAIV against Wuhan and Delta virus observed in the hamster challenge experiments can be explained by different pathogenicity of the two SARS-CoV-2 variants for these animals, as we noted the milder course of the disease after infection with Delta strain, compared to the Wuhan virus, and the non-specific activation of T cells could be more effective in case of lighter infection.

Conclusions
Overall, in this study we designed a T cell-based COVID-19 vaccine by inserting a polyepitope SARS-CoV-2 cassette into genome of LAIV virus. Assessment of the selected Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/vaccines10071142/s1, Figure S1: 504-522 fragment of spike protein aligned with peptides established as immunogenic/MHC molecule binding epitopes in experimental studies; Figure S2: 947-1020 fragment of spike protein aligned with peptides established as immunogenic/MHC molecule binding epitopes in experimental studies; Figure S3: 1056-1078 fragment of spike protein aligned with peptides established as immunogenic/MHC molecule binding epitopes in experimental studies; Figure S4: 1180-1231 fragment of spike protein aligned with peptides established as immunogenic/MHC molecule binding epitopes in experimental studies; Figure S5: 92-170 fragment of nucleocapsid protein aligned with peptides established as immunogenic/MHC molecule binding epitopes in experimental studies; Figure S6: 214-235 fragment of nucleocapsid protein aligned with peptides established as immunogenic/MHC molecule binding epitopes in experimental studies; Figure S7: 293-370 fragment of nucleocapsid protein aligned with peptides established as immunogenic/MHC molecule binding epitopes in experimental studies; Figure S8: 42-79 fragment of membrane protein aligned with peptides established as immunogenic/MHC molecule binding epitopes in experimental studies; Figure S9: Expression of the HLA-A2 molecules on the mouse splenic cells and HLA-A*02:01 positive donor as detected using the PE Anti-HLA A2 antibody [BB7.2]; Figure S10: Levels of IFNγ-producing cytotoxic T cells after stimulation of mouse splenocytes with CEF peptide mix; Figure S11: Levels of cytokine-producing cytotoxic T cells after stimulation of mouse splenocytes with influenza NP366 peptide; Figure S12: The levels of IFNγ-producing helper (A) and effector memory (B) T cell subsets in PBMC samples of naïve individuals stimulated in vitro with the studied recombinant LAIV/SARS-CoV-2 viruses; Figure S13: Histopathological evaluation of lung tissues of immunized Syrian hamsters on day 5 after challenge with SARS-CoV-2 Wuhan virus; Figure S14. Overview of histopathological changes in lung tissues of immunized Syrian hamsters on day 5 after challenge with SARS-CoV-2 Delta variant; Figure S15. Overview of histopathological changes in lung tissues of immunized Syrian hamsters on day 5 after challenge with SARS-CoV-2 Wuhan variant. Table S1: A list of HLA-A*02:01 restricted peptides used to assess T-cell immune responses to SARS-CoV-2 virus in HLA-A2.1 transgenic mice; Table S2: Demographic characteristics of COVID-19 patients who donated whole blood for this study; Table  S3: Epitopes in 504-522 fragment of spike protein, deposited in IEDB, additional information and references; Table S4: Epitopes in 947-1020 fragment of spike protein, deposited in IEDB, additional information and references; Table S5: Epitopes in 1056-1078 fragment of spike protein, deposited in IEDB, additional information and references; Table S6: Epitopes in 1056-1078 fragment of spike protein, deposited in IEDB, additional information and references; Table S7: Epitopes in 92-170 fragment of nucleocapsid protein, deposited in IEDB, additional information and references; Table S8: Epitopes in 214-235 fragment of nucleocapsid protein, deposited in IEDB, additional information and references; Table S9: Epitopes in 214-235 fragment of nucleocapsid protein, deposited in IEDB, additional information and references; Table S10. Epitopes in 42-79 fragments of membrane protein, deposited in IEDB, additional information and references.  Informed Consent Statement: Informed consent was obtained from all subjects involved in the study.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.