Involvement of l-Cysteine Desulfhydrase and Hydrogen Sulfide in Glutathione-Induced Tolerance to Salinity by Accelerating Ascorbate-Glutathione Cycle and Glyoxalase System in Capsicum

The aim of this study is to assess the role of l-cysteine desulfhydrase (l-DES) and endogenous hydrogen sulfide (H2S) in glutathione (GSH)-induced tolerance to salinity stress (SS) in sweet pepper (Capsicum annuum L.). Two weeks after germination, before initiating SS, half of the pepper seedlings were retained for 12 h in a liquid solution containing H2S scavenger, hypotaurine (HT), or the l-DES inhibitor dl-propargylglycine (PAG). The seedlings were then exposed for three weeks to control or SS (100 mmol L−1 NaCl) and supplemented with or without GSH or GSH+NaHS (sodium hydrosulfide, H2S donor). Salinity suppressed dry biomass, leaf water potential, chlorophyll contents, maximum quantum efficiency, ascorbate, and the activities of dehydroascorbate reductase, monodehydroascorbate reductase, and glyoxalase II in plants. Contrarily, it enhanced the accumulation of hydrogen peroxide, malondialdehyde, methylglyoxal, electrolyte leakage, proline, GSH, the activities of glutathione reductase, peroxidase, catalase, superoxide dismutase, ascorbate peroxidase, glyoxalase I, and l-DES, as well as endogenous H2S content. Salinity enhanced leaf Na+ but reduced K+; however, the reverse was true with GSH application. Overall, the treatments, GSH and GSH+NaHS, effectively reversed the oxidative stress and upregulated salt tolerance in pepper plants by controlling the activities of the AsA-GSH and glyoxalase-system-related enzymes as well as the levels of osmolytes.


Introduction
Sweet pepper is an important vegetable crop, especially in the Mediterranean region. It is usually grown in greenhouses to obtain high yields and good quality fruit in comparison with that from field conditions [1]. It is believed that sweet pepper can moderately tolerate saline stress, which is ascribed to reduced plant growth and yield [2,3].
Salinized soil and saltwater are the main reasons for the reduced production of most crops [4,5]. Salt stress causes a buildup of sodium (Na + ) and chloride (Cl − ), which causes osmotic and ionic stresses in plants [6,7]. This leads to detrimental effects on both metabolic and biochemical events in plants, and thus causes the inhibition of plant growth and development [8]. Salinity also decreases the uptake of water and essential nutrient elements [9], photosynthesis and pigment synthesis [10] and enzyme activity, as well as the accumulation of secondary metabolites [11]. One of the crucial harmful effects of salinity is oxidative impairment due to a marked buildup of reactive oxygen species (ROS) such as Details of Hoagland's nutrient solution (NS) are highlighted by Kaya and Ashraf [43]; the NS' pH was kept at 5.5. The NS was applied to the root growth medium of plants every other day during the experimentation. Figure 1 shows the scheme of the treatments used to assess the effect of salinity stress (SS) on sweet pepper plants. Two weeks after germination, plants were grown in NS without adding NaCl (control) or by adding 100 mmol L −1 NaCl (SS) for three weeks, and during the same period, glutathione (1.0 mmol L −1 GSH) or GSH+NaHS (1.0 mmol L −1 GSH + 0.2 mmol L −1 sodium hydrosulfide, H 2 S donor) was supplemented via nutrient solution. Furthermore, two weeks after germination, half of the young seedlings were retained for 12 h in an aqueous solution consisting of the H 2 S scavenger, hypotaurine (0.1 mmol L −1 HT), or l-DES inhibitor, dl-propargylglycine (0.3 mmol L −1 PAG).
Antioxidants 2020, 9, x FOR PEER REVIEW 3 of 30 Figure 1 shows the scheme of the treatments used to assess the effect of salinity stress (SS) on sweet pepper plants. Two weeks after germination, plants were grown in NS without adding NaCl (control) or by adding 100 mmol L −1 NaCl (SS) for three weeks, and during the same period, glutathione (1.0 mmol L −1 GSH) or GSH+NaHS (1.0 mmol L −1 GSH + 0.2 mmol L −1 sodium hydrosulfide, H2S donor) was supplemented via nutrient solution. Furthermore, two weeks after germination, half of the young seedlings were retained for 12 h in an aqueous solution consisting of the H2S scavenger, hypotaurine (0.1 mmol L −1 HT), or L-DES inhibitor, DL-propargylglycine (0.3 mmol L −1 PAG). The duration and concentrations of HT and PAG were selected according to the work of Li et al. [44] for maize plants exposed to high temperatures. A dose of 1 mmol L −1 GSH was used for mung bean [30] and soybean [45] under saline stress. These concentrations were selected for pepper plants in the present experiment.
Each treatment was replicated thrice, and each replicate comprised 9 plants, i.e., 27 plants for each treatment. Salinity stress of the root-growing medium was maintained constant throughout the experimentation by draining the excess amounts of NS through the plastic pots; this also prevented waterlogging.
Twenty-one days after initiating the salinity stress treatment, 3 plants from each replication were uprooted and separated into shoots and roots to measure dry mass. The separated plant parts were subjected to 75 °C in an oven for three days to determine dry mass. The remaining 6 plants from each replication were taken to determine the following parameters.

Photosynthetic Pigments and Chlorophyll Fluorescence
The protocol of Strain and Svec [46] was followed to quantify chlorophyll content. One g of fresh leaf tissue was macerated using acetone solution (5 mL, 90% v/v). The extracted sample solution was read at 663.5 and 645 nm for Chl a and Chl b, respectively.
For assessing the extent of chlorophyll fluorescence, leaf samples adapted at dark for 30 min were used. All fluorescence measurements were made with a photosynthesis yield analyzer (Mini-PAM, Walz, Germany).

Leaf Water Potential and Relative Water Content (RWC)
The protocol of Yamasaki and Dillenburg [47] was employed to determine RWC. Further details of the protocol are given by Kaya et al. [48].
For water-potential measurements, a fully expanded youngest leaf excised from each plant in the morning was subjected to a water-potential apparatus (a pressure chamber, PMS model 600, USA). The duration and concentrations of HT and PAG were selected according to the work of Li et al. [44] for maize plants exposed to high temperatures. A dose of 1 mmol L −1 GSH was used for mung bean [30] and soybean [45] under saline stress. These concentrations were selected for pepper plants in the present experiment.

Proline Content
Each treatment was replicated thrice, and each replicate comprised 9 plants, i.e., 27 plants for each treatment. Salinity stress of the root-growing medium was maintained constant throughout the experimentation by draining the excess amounts of NS through the plastic pots; this also prevented waterlogging.
Twenty-one days after initiating the salinity stress treatment, 3 plants from each replication were uprooted and separated into shoots and roots to measure dry mass. The separated plant parts were subjected to 75 • C in an oven for three days to determine dry mass. The remaining 6 plants from each replication were taken to determine the following parameters.

Photosynthetic Pigments and Chlorophyll Fluorescence
The protocol of Strain and Svec [46] was followed to quantify chlorophyll content. One g of fresh leaf tissue was macerated using acetone solution (5 mL, 90% v/v). The extracted sample solution was read at 663.5 and 645 nm for Chl a and Chl b, respectively.
For assessing the extent of chlorophyll fluorescence, leaf samples adapted at dark for 30 min were used. All fluorescence measurements were made with a photosynthesis yield analyzer (Mini-PAM, Walz, Germany).

Leaf Water Potential and Relative Water Content (RWC)
The protocol of Yamasaki and Dillenburg [47] was employed to determine RWC. Further details of the protocol are given by Kaya et al. [48]. For water-potential measurements, a fully expanded youngest leaf excised from each plant in the morning was subjected to a water-potential apparatus (a pressure chamber, PMS model 600, USA).

Proline Content
The procedure outlined by Bates et al. [49] was followed to determine leaf free proline.

Glycine Betaine (GB) Determination
GB content was determined, as described by Grieve and Grattan [50]. The initially dried leaf samples were ground to powder, and then, 0.5 g of the dried sample was extracted in 20 mL of deionized water by shaking at 25 • C for 24 h. The sample mixture was then treated with 2N H 2 SO 4 (1:1). Then, 0.5-mL of the extract was added to 0.2 mL cold potassium iodide to start the reaction. The treated sample solution was centrifuged properly at 10,000× g for 15 min. The aliquot was added to 1,2-dichloroethane, and absorbance readings were recorded at 365 nm after 3 h. The standards of GB were prepared in 2N H 2 SO 4 at a range of 50-200 µg mL −1 .

Determination of l-Cysteine Desulfhydrase Activity (l-DES) and Hydrogen Sulfide (H 2 S) Content
The l-DES activity was determined by quantifying H 2 S release from l-cysteine in the presence of dithiothreitol (DTT) by employing the method of Li et al. [51]. A leaf tissue (5 g) was triturated with liquid N 2 , and the soluble protein was extracted in a 5-mL solution of Tris-HCl (20 mmol L −1 , pH 8.0). With the objective of achieving the same amount of protein in each sample, an adjustment of the protein content in the treated sample solution was made at 100 µg mL −1 . An aliquot (1 mL) of the extracted sample contained 0.8 mmol L −1 l-cysteine, 100 mmol L −1 Tris-HCl, 2.5 mmol L −1 DTT at pH 9.0, and a 10-µg protein solution. By adding l-cysteine to the sample solution, the reaction was initiated, and then the treated sample was incubated for 15 min at 37 • C. An aliquot (0.1 mL) of 30 mmol L −1 FeCl 3 was treated with 1.2 mol L −1 HCl, and 0.1 mL of 20 mmol L −1 N, N-dimethyl-p-phenylenediamine dihydrochloride. Lastly, it was added to the extract. The optical density of the samples was quantified at 667 nm after leaving it at room temperature for 15 min.
Leaf H 2 S was quantified following the protocol outlined by Li et al. [51], which is based on the formation of methylene blue from dimethyl-p-phenylenediamine in H 2 SO 4 .

Quantification of H 2 O 2 Levels
The Velikova et al. [52] protocol was pursued to determine the levels of leaf H 2 O 2 . A proportion of fresh leaf (0.5 g) was macerated in TCA (trichloroacetic acid, 3 mL of 1% w/v) and then 0.75 mL of aliquot was added to the potassium-phosphate buffer (0.75 mL of 10 mmol L −1 , pH 7.0) and KI (1.5 mL of 1 mol L −1 ) solution. The OD of all treated samples was recorded at 390 nm.

Malondialdehyde (MDA) Assay
The leaf MDA was assayed per the procedure outlined by Heath and Packer [53]. A 100-mg fresh leaf was triturated in trichloroacetic acid (TCA, 0.5 mL 0.1% w/v), and then the sample extract was centrifuged for 10 min at 15,000× g at 4 • C. Thereafter, the aliquot was diluted using TCA (20%), and 0.5 mL of 1.5% (w/v) thiobarbituric acid (TBA) was added to the aliquot mixture, and then it was kept in a water bath at 95 • C for 25 min. The OD of all treated samples was noted at 532 nm after cooling down the final sample on ice.

Electrolyte Leakage (EL)
The method previously outlined by Dionisio-Sese and Tobita [54] was followed to measure EL.

Soluble Proteins and Antioxidant Enzymes
Briefly, 0.5 g of fresh leaf tissue was macerated in 50 mmol L −1 sodium-phosphate buffer, which consisted of 1% (w/v) soluble polyvinyl pyrrolidine. The homogenized solution was subjected to centrifugation at 20,000× g at 4 • C for 15 min. The catalase (CAT) activity was assayed as described by Kraus and Fletcher [55]. The peroxidase (POD) activity was determined as illustrated by Chance and Maehly [56], and that of SOD was determined by quantifying the SOD ability to inhibit the nitroblue tetrazolium (NBT) photochemical [57]. Total soluble proteins were quantified, as illustrated by Bradford [58].

Analysis of Ascorbate (AsA) and Glutathione (GSH)
A fresh leaf tissue (0.5 g) was used to quantify AsA and GSH contents. The samples were triturated using a cold mixture of metaphosphoric acid (3 mL of 5% w/v) and EDTA (1.0 mol L −1 ). Thereafter, the homogenized solution was subjected to centrifugation at 11,500× g for 12 min at 4 • C, and the supernatant was pipetted out for the determination of both GSH and AsA.
The quantification of AsA level was done per the procedure of Huang et al. [59]. Then, 0.4 mL of the sample aliquot was neutralized with 0.6 mL of 500 mmol L −1 potassium-phosphate buffer (pH 7.0), and then it was determined in 100 mmol L −1 potassium-phosphate buffer (at pH 7.0), including 0.5-unit ascorbate oxidase at 265 nm.
The quantification of glutathione disulfide (GSSG) and oxidized GSH was assayed, employing the procedure of Yu et al. [60]. An aliquot (0.4 mL) of the sample solution was added to 0.6 mL of the potassium-phosphate buffer (500 mmol L −1 at pH 7.0). Glutathione is estimated by the change in absorption rate at 412 nm for NTB (2-nitro-5-thiobenzoic acid), a product which results in the DTNB [5,5 -dithio-bis (2-nitrobenzoic acid)] reduction. The concentration of GSSG was appraised by abolishing GSH with a derivatizing agent, 2-vinylpyridine.

Plant Crude Extracts
A protocol described by Shan and Liang [61] was used to estimate the enzymes' activities. Each single leaf sample (0.5 g) was ground to powder using liquid nitrogen, and then, the sample was added to a test tube to which 6 mL of ice-cold extract solution consisting of 50 mmol L −1 KH 2 PO4 (pH 7.5), 0.1 mmol L −1 ethylenediaminetetraacetic acid (EDTA), Triton X-100 (0.3% v/v), and soluble polyvinylpolypyrrolidone (1% w/v) was added. For the APX assay, 1 mmol L −1 AsA was also used. The sample mixtures were rapidly centrifuged for 15 min at 13,000× g at 2 • C, and then the following enzymes were assayed rapidly in this aliquot.

Enzyme Assay
A protocol described by Nakano and Asada [62] was adopted to quantify the APX activity by following a decrease in optical density at 290 nm. The sample mixture contained 50 mM phosphate buffer (pH 7.3), 1 mmol L −1 AsA, 0.1 mmol L −1 EDTA, 1 mmol L −1 H 2 O 2 , and the enzyme extract.
A protocol illustrated by Grace and Logan [63] was used to estimate the GR activity by following a decline in OD at 340 nm. A reaction solution was prepared, which comprised 100 mmol L −1 Tris-HCl (pH 8.0), 0.5 mmol L −1 EDTA, 0.5 mmol L −1 MgCl 2 , 10 mmol L −1 oxidized glutathione (GSSG), 1 mmol L −1 NADPH, and the enzyme extract.
Glyoxalase I (Gly I) activity was quantified employing the protocol outlined by Hasanuzzaman et al. [66]. A solution of 100 mmol L −1 potassium-phosphate buffer at pH 7.0, comprising 15 mmol L −1 magnesium sulfate, 1.7 mmol L −1 GSH, and 3.5 mmol L −1 MG, was reacted with the enzyme assay solution. Optical density was read at 240 nm. For the quantification of glyoxalase II (Gly II) activity, the protocol delineated by Hasanuzzaman et al. [66] was adopted. A solution containing 100 mmol L −1 Tris-HCl buffer (pH 7.2), 0.2 mmol L −1 DTNB, and 1.0 mmol L −1 S-d-lactoylglutathione (SLG) was poured into the enzyme assay solution. Then, the OD values were read at 240 nm and the Gly-II activity estimated.

Methylglyoxal (MG) Levels
After homogenizing 0.5 g of the leaf sample 1:1 (w/v) in 5% perchloric acid, the homogenized solution was subjected to centrifugation at 11,000× g and 4 ºC for 10 min. Charcoal was mixed in the supernatant to abolish the color, and then a saturated potassium carbonate solution was also used to neutralize it at 25 • C. The N-acetyl-l-cysteine and sodium dihydrogen phosphate were added to the treated sample solution to attain a final volume of 1 mL. After 10 min, the synthesis of N-α-acetyl-S-(1-hydroxy-2-oxo-prop-1-yl) cysteine was noted at 288 nm [67].

Quantification of Mineral Nutrients
The powdered dry leaf tissues were ashed by subjecting all samples to 550 • C in a muffle furnace. The ash so obtained was mixed in 5 mL of 2 mol L −1 hot HCl for the estimation of Na + and K + contents [68]. Sodium (Na + ) and K + in the samples were analyzed with an ICP-OES (PerkinElmer Optima 5300 DV). The conditions of ICP-OES were RF Power 1.35 kW, plasma gas flow rate 12.1 L min −1 , sample solution uptake rate 1.5 mL min −1 , carrier gas flow rate 0.5 mL min −1 , nebulizer gas flow rate 0.85 L min −1 , and wavelengths 766.490 and 588.021 for K and Na, respectively.

Statistical Treatment to Data of All Attributes
The data for various variables were analyzed following an analysis of variance. Mean data, along with respective standard errors of each variable, were presented as bar diagrams. The mean values were compared for significant differences with Duncan's multiple range test at p ≤ 0.001 level of probability.

Treatment of GSH Enhances Plant Growth
Significant reductions (p ≤ 0.001) were obtained in shoot, root, and total dry weight of plants by 45.8%, 38.1%, and 43.9%, respectively, in the salt-stressed sweet pepper plants relative to the control. Decreases in these variables were significantly (p ≤ 0.001) raised by 31.2%, 29.4%, and 30.7%, respectively, with the application of GSH. Furthermore, increases in these variables were higher due to the treatment of GSH+NaHS relative to those in the salinity-stressed plants receiving no supplementation of GSH+NaHS (Figure 2A-C). The supplementation of l-DES inhibitor PAG or HT, in combination with GSH, totally reversed the positive effects of GSH on the plant growth parameters in the SS-plants by reducing the l-DES activity and H 2 S synthesis (as shown in the latter section), which suggests that the l-DES activity and synthesis of H 2 S participate in GSH-induced increased plant growth under SS. Furthermore, although HT reversed the beneficial role of GSH+NaHS, PAG did not completely reverse these effects under saline conditions. However, these different treatments did not affect those attributes in the control plants, showing that GSH or GSH+NaHS was only effective under stress conditions.

GSH Improves Photosynthesis-Related Parameters
Relative to the control plants, significant decreases, such as 33.4%, 26.8%, and 24.9% in Chl a and Chl b contents and photosystem II efficiency (Fv/Fm), respectively, were observed in the salt-stressed plants ( Figure 3A

GSH Improves Photosynthesis-Related Parameters
Relative to the control plants, significant decreases, such as 33.4%, 26.8%, and 24.9% in Chl a and Chl b contents and photosystem II efficiency (F v /F m ), respectively, were observed in the salt-stressed plants ( Figure 3A-C). However, salt-stressed plants supplemented with GSH exhibited higher Chl a, Chl b, and F v /F m by 32.6%, 23.0%, and 26.4%, respectively, over those in the controls. Furthermore, the GSH+NaHS treatment led to a further increase in the photosynthesis-related parameter. Application of PAG and HT both reversed the mitigation effect of GSH on these attributes, showing that GSH-induced improvement in l-DES activity and H 2 S synthesis improves photosynthesis-related parameters under saline conditions. The mitigation effect of GSH+NaHS was completely reversed by the HT supply, thereby reducing the H 2 S content without inhibiting l-DES activity. However, this was partly reversed by PAG by completely blocking l-DES activity and partly reducing H 2 S, showing that H 2 S plays a vital role as a signal molecule in GSH-induced improvement in the photosynthesis-related attributes. However, all different treatments used did not alter those parameters in the control plants, showing that those treatments function only under stress conditions. Antioxidants 2020, 9, x FOR PEER REVIEW 8 of 30 Chl b, and Fv/Fm by 32.6%, 23.0%, and 26.4%, respectively, over those in the controls. Furthermore, the GSH+NaHS treatment led to a further increase in the photosynthesis-related parameter. Application of PAG and HT both reversed the mitigation effect of GSH on these attributes, showing that GSHinduced improvement in L-DES activity and H2S synthesis improves photosynthesis-related parameters under saline conditions. The mitigation effect of GSH+NaHS was completely reversed by the HT supply, thereby reducing the H2S content without inhibiting L-DES activity. However, this was partly reversed by PAG by completely blocking L-DES activity and partly reducing H2S, showing that H2S plays a vital role as a signal molecule in GSH-induced improvement in the photosynthesisrelated attributes. However, all different treatments used did not alter those parameters in the control plants, showing that those treatments function only under stress conditions.

GSH Improves Leaf Water Relations and Proline and Glycine Betaine Contents
The water-related parameters, such as leaf relative water contents (RWC) and leaf water potential (ΨI), declined by 26.1% and 195.9%, respectively, in the salt-stressed plants over the controls. However, salt-stressed plants fed with GSH exhibited a lower reduction of 9.6% in RWC and 142.4% in ΨI relative to those in the controls ( Figure 4A,B). The treatment of GSH+NaHS led to further increases in both RWC and ΨI of plants under SS. Salinity stress enhanced proline and glycine betaine (GB) contents by 2.9-and 2.6-fold, respectively, in the pepper plants, relative to those in the control treatment. The supplementation of the salt-stressed plants with GSH and GSH+NaHS led to a further rise in proline by 3.9-and 4.7-fold, and GB by 3.5-and 4.2-fold, respectively, over those in the control plants ( Figure 4C,D). These attributes in the respective control plants remained unchanged under varying treatment regimes.

GSH Improves Leaf Water Relations and Proline and Glycine Betaine Contents
The water-related parameters, such as leaf relative water contents (RWC) and leaf water potential (ΨI), declined by 26.1% and 195.9%, respectively, in the salt-stressed plants over the controls. However, salt-stressed plants fed with GSH exhibited a lower reduction of 9.6% in RWC and 142.4% in ΨI relative to those in the controls ( Figure 4A,B). The treatment of GSH+NaHS led to further increases in both RWC and ΨI of plants under SS. Salinity stress enhanced proline and glycine betaine (GB) contents by 2.9-and 2.6-fold, respectively, in the pepper plants, relative to those in the control treatment. The supplementation of the salt-stressed plants with GSH and GSH+NaHS led to a further rise in proline by 3.9-and 4.7-fold, and GB by 3.5-and 4.2-fold, respectively, over those in the control plants ( Figure 4C,D). These attributes in the respective control plants remained unchanged under varying treatment regimes.  The beneficial effect of GSH on these attributes was totally inverted by PAG and HT by reducing both the activity of l-DES enzyme and H 2 S synthesis, showing that both l-DES enzyme and H 2 S synthesis are required in GSH-induced improvement in water status, and proline and GB contents under SS conditions. Furthermore, the alleviation effect of GSH+NaHS was completely prevented by HT by reducing NaHS without reducing the l-DES activity; it was partly prevented by PAG-induced reduction in the l-DES activity without completely reducing H 2 S.

GSH Improves l-DES Activity and H 2 S Synthesis in Pepper Plants under SS
Salinity stress led to marked (p ≤ 0.001) increases in the activity of l-DES and synthesis of H 2 S by 53.5% and 50.4%, respectively, relative to those in the control treatment ( Figure 5A,B). The supplementation of GSH resulted in a further significant (p ≤ 0.001) improvement in l-DES activity and led to a rise in the levels of endogenous H 2 S in the SS-pepper plants. These results indicate that GSH might have increased the accumulation of H 2 S by stimulating l-DES activity in the pepper seedlings under salinity stress. Application of GSH+NaHS further increased the H 2 S content but reduced the l-DES activity.
The beneficial effect of GSH on these attributes was totally inverted by PAG and HT by reducing both the activity of L-DES enzyme and H2S synthesis, showing that both L-DES enzyme and H2S synthesis are required in GSH-induced improvement in water status, and proline and GB contents under SS conditions. Furthermore, the alleviation effect of GSH+NaHS was completely prevented by HT by reducing NaHS without reducing the L-DES activity; it was partly prevented by PAG-induced reduction in the L-DES activity without completely reducing H2S.

GSH Improves L-DES Activity and H2S Synthesis in Pepper Plants under SS
Salinity stress led to marked (p ≤ 0.001) increases in the activity of L-DES and synthesis of H2S by 53.5% and 50.4%, respectively, relative to those in the control treatment ( Figure 5A,B). The supplementation of GSH resulted in a further significant (p ≤ 0.001) improvement in L-DES activity and led to a rise in the levels of endogenous H2S in the SS-pepper plants. These results indicate that GSH might have increased the accumulation of H2S by stimulating L-DES activity in the pepper seedlings under salinity stress. Application of GSH+NaHS further increased the H2S content but reduced the L-DES activity. To assess the effect of the inhibitor of l-DES and the scavenger of H 2 S on GSH-induced improvement in salt tolerance in the sweet pepper plants, the plants were treated with GSH alone or jointly with NaHS, PAG, or HT. In the salt-stressed plants treated with GSH alone, the application of PAG totally prevented the increase in l-DES activity and H 2 S synthesis, but HT reversed the H 2 S content without reducing the l-DES activity. However, in the salt-stressed plants treated with GSH+NaHS, the PAG treatment totally reversed the l-DES activity without reducing the H 2 S content, and HT treatment prevented the increase in H 2 S content and l-DES activity. These results showed that PAG reduced the total l-DES activity, and in that way, it deactivated H 2 S synthesis, but HT directly scavenged H 2 S without hampering the l-DES activity. Furthermore, when NaHS was supplied externally along with GSH, PAG did not successfully reduce the H 2 S content. Our results also showed that H 2 S was produced by l-DES, which was activated by GSH under SS conditions.

GSH-Induced Rise in l-DES Activity and H 2 S Synthesis Relieve Oxidative Stress and Enhance Antioxidant Defence System in Pepper Plants under SS
The salt-stressed pepper plants exhibited a significant rise in oxidative stress-related parameters H 2 O 2 , MDA, and EL by 2.1-, 2.0-, and 4.9-fold, respectively, relative to those in the control plants ( Figure 6A-C). Application of GSH to the salt-stressed plants reduced the accumulation of H 2 O 2 and MDA and EL values by 30.6%, 26.2%, and 53.3%, respectively, over those in the SS-plants alone. Furthermore, the GSH and NaHS treatment further reduced the oxidative stress parameters, showing that the external application of NaHS along with GSH had a positive effect on reversing the oxidative stress. In the control plants, the oxidative stress-related parameters were not affected by various treatments.
However, PAG or HT treatment, along with GSH (GSH+PAG or GSH+HT), totally reversed the reduced oxidative stress due to GSH in the salt-stressed plants by reducing the H 2 S content and l-DES activity with GSH+PAG, and reducing H 2 S without reducing l-DES activity with the GSH+HT treatment. The reduced oxidative stress due to GSH+NaHS was not eliminated by PAG, even though PAG reduced the l-DES activity. However, the positive response in reducing oxidative stress due to the GSH+NaHS treatment was totally reversed by HT, thereby reducing the H 2 S content without reducing the l-DES activity.
Salinity stress significantly (p ≤ 0.001) increased the activities of antioxidant defense system-related enzymes, such as SOD, CAT, and POD by 2.1-, 2.9-and 1.9-fold, relative to those in the nonstressed plants ( Figure 6D-F). The treatments of GSH and GSH+NaHS led to further increases in SOD by 19.2% and 30.4%, CAT by 15.6% and 29.8%, and POD by 17.1% and 26.1%, respectively, over those in the SS-plants, without being treated with GSH or GSH+NaHS. However, the upregulating effect of GSH on the antioxidant defense systems in the SS-plants was reversed by PAG or HT, thereby reducing the H 2 S content. The positive response of GSH+NaHS on the antioxidant defense system was not completely abolished by PAG, but was completely eliminated by HT, thereby reducing the H 2 S content.

The Participation of l-DES Activity and Endogenous H 2 S in GSH-Induced Regulation of Nonenzymatic Antioxidants in SS-Pepper Plants
Plants subjected to SS showed a significant decrease in ascorbate (AsA) content by 33.4% but increases in the production of GSH and GSSG in the leaves of the plants under SS by 59.2% and 30.2%, respectively, relative to those under the control treatment ( Figure 7A-C). The treatment of GSH led to significant increases in AsA and GSH contents by 71.5% and 112.8%, respectively, but decreases in GSSG content by 21.2% in the salt-stressed pepper compared to those in the plants treated only with salt. In the case of GSH+NaHS supplied to the SS-plants, significant increases were also observed in the AsA and GSH contents by 2.5-, and 2.2-fold, respectively; however, there were significant decreases in GSSG content by 34.1% in the SS-plants relative to those in the SS-plants receiving no supply of GSH+NaHS. The ratio of GSH/GSSG did not differ significantly under SS relative to the control. However, relative to SS alone, the exogenously applied GSH and GSH+NaHS significantly enhanced the GSH/GSSG ratio by 2.1-and 3.7-fold, respectively ( Figure 7D). However, PAG or HT treatment, along with GSH (GSH+PAG or GSH+HT), totally reversed the reduced oxidative stress due to GSH in the salt-stressed plants by reducing the H2S content and L-DES activity with GSH+PAG, and reducing H2S without reducing L-DES activity with the GSH+HT treatment. The reduced oxidative stress due to GSH+NaHS was not eliminated by PAG, even though PAG reduced the L-DES activity. However, the positive response in reducing oxidative stress due to the GSH+NaHS treatment was totally reversed by HT, thereby reducing the H2S content without reducing the L-DES activity.  PAG or HT supplemented to the SS-plants treated with GSH alone completely eliminated the positive effect of GSH by reducing AsA and GSH and lowering the GSSG levels, as well as reducing the GSH/GSSG ratio. Although the reversing effect of HT on the SS-plants treated with GSH+NaHS was similar to that of its effect on the plants treated with GSH alone, PAG did not show a complete reversing effect on AsA, GSH, and GSSG in the salt-stressed plants treated with GSH+NaHS.

The Participation of l-DES Activity and Endogenous H 2 S in GSH-Induced Upregulation of Ascorbate-Glutathione (AsA-GSH) Cycle in SS-Pepper Plants
To understand to what extent l-DES and H 2 S participate in SA-induced enhancement in salt stress tolerance by upregulating the AsA-GSH cycle in the pepper plants, alterations in the AsA-GSH-cycle-related enzymes' activities were quantified in the leaves of the pepper seedlings.
The salt-stressed plants showed enhanced activities of APX and GR by 91.65% and 90.1%, but reduced DHAR and MDHAR by 35.1% and 45.2%, respectively, over the controls (Figure 8A-D). The supply of GSH to the SS plants led to increased activities of APX, GR, DHAR, and MDHAR by 17.2%, 17.1%, 93.9%, and 90.9%, respectively, relative to those in the salt-stressed plants fed with no GSH. The treatment of GSH+NaHS led to further increases in these enzymes' activities by 27.3%, 26.9%, 125.2%, and 139.4%, respectively, in comparison with those in the SS-plants alone.
Antioxidants 2020, 9,  Application of PAG or HT, along with GSH, completely prevented the alleviation effects of GSH on the previously mentioned traits in the SS-plants. This suggests that GSH might have activated the L-DES activity, which is the potential source of H2S, so H2S-induced upregulation of the AsA-GSHcycle-related enzymes' activities might have induced tolerance to SS in the pepper seedlings. When PAG or HT was applied along with the GSH+NaHS treatments, the upregulatory effects of GSH+NaHS on the AsA-GSH-cycle-related enzymes' activities were completely reversed by HT, thereby reducing H2S without reducing the activity of L-DES; however, it was partly reversed by PAG, which reduced the accompanied L-DES activity by partly reducing H2S. This also suggests that H2S content is needed, but L-DES activity is not needed for the upregulation of the AsA-GSH-cyclerelated enzymes due to GSH+NaHS under SS conditions, as long as the donor of H2S is supplied externally. These parameters remained unaltered in the control plants by different treatments. Application of PAG or HT, along with GSH, completely prevented the alleviation effects of GSH on the previously mentioned traits in the SS-plants. This suggests that GSH might have activated the l-DES activity, which is the potential source of H 2 S, so H 2 S-induced upregulation of the AsA-GSH-cycle-related enzymes' activities might have induced tolerance to SS in the pepper seedlings. When PAG or HT was applied along with the GSH+NaHS treatments, the upregulatory effects of GSH+NaHS on the AsA-GSH-cycle-related enzymes' activities were completely reversed by HT, thereby reducing H 2 S without reducing the activity of l-DES; however, it was partly reversed by PAG, which reduced the accompanied l-DES activity by partly reducing H 2 S. This also suggests that H 2 S content is needed, but l-DES activity is not needed for the upregulation of the AsA-GSH-cycle-related enzymes due to GSH+NaHS under SS conditions, as long as the donor of H 2 S is supplied externally. These parameters remained unaltered in the control plants by different treatments.

GSH-Induced Enhanced l-DES Activity and H 2 S Synthesis Reduces Sodium and Improves Potassium and Calcium Contents in SS-Pepper Plants
Saline stress caused a higher accumulation of Na + in the leaves by 4.29-fold relative to the controls ( Figure 9A). However, significant decreases were observed in the leaf K + contents of the SS-plants by 41.9% over the controls ( Figure 9B). The leaf K + /Na + ratio ( Figure 9C) was reduced in the salt-stressed plants by 7.4-fold over the controls.

GSH-Induced Enhanced L-DES Activity and H2S Synthesis Reduces Sodium and Improves Potassium and Calcium Contents in SS-Pepper Plants
Saline stress caused a higher accumulation of Na + in the leaves by 4.29-fold relative to the controls ( Figure 9A). However, significant decreases were observed in the leaf K + contents of the SSplants by 41.9% over the controls ( Figure 9B). The leaf K + /Na + ratio ( Figure 9C) was reduced in the salt-stressed plants by 7.4-fold over the controls. The supplementation of GSH lowered the concentration of Na + in the leaves by 50.9% in the SSplants relative to those in the SS-plants alone. However, the same treatment led to increases in leaf K + content and K + /Na + ratio by 51.1% and 206.3%, respectively, in the plants treated with NaCl.
In order to provide further evidence that H2S is involved in GSH-induced SS tolerance in the pepper plants, NaHS (a donor of H2S) was supplied externally along with GSH (GSH+NaHS). The plants treated with GSH and NaHS in combination with GSH+NaHS led to further increases in the leaf K + content and K + /Na + ratio and a decrease in Na + content. These results indicate that externally The supplementation of GSH lowered the concentration of Na + in the leaves by 50.9% in the SS-plants relative to those in the SS-plants alone. However, the same treatment led to increases in leaf K + content and K + /Na + ratio by 51.1% and 206.3%, respectively, in the plants treated with NaCl.
In order to provide further evidence that H 2 S is involved in GSH-induced SS tolerance in the pepper plants, NaHS (a donor of H 2 S) was supplied externally along with GSH (GSH+NaHS). The plants treated with GSH and NaHS in combination with GSH+NaHS led to further increases in the leaf K + content and K + /Na + ratio and a decrease in Na + content. These results indicate that externally applied NaHS makes GSH more effective in improving the K + content and lowering the Na + content, and in this way, it increased the tolerance to SS in the pepper plants. In the salt-stressed pepper plants treated with GSH, the PAG or HT treatment completely reversed the decline in Na + content and the rise in K + content due to GSH, possibly by suppressing l-DES activity and H 2 S content or reducing the H 2 S content alone without reducing the l-DES activity. This provides evidence that endogenous H 2 S may be is involved in GSH-induced regulation of salt stress tolerance. Furthermore, for l-DES playing a key role in producing H 2 S signal molecules, the additional evidence proves that blocking the activity of l-DES by using its inhibitor leads to a decrease in H 2 S and reverses the positive effect of GSH. However, in the case of supplementing NaHS externally along with GSH, the PAG and HT treatments showed different effects in reversing the positive effect of GSH+NaHS. The treatment of HT reversed the positive effect of GSH+NaHS on K + uptake by reducing the H 2 S content without reducing the l-DES enzyme activity. The PAG was less effective in reversing the positive effect of GSH+NaHS on K + uptake, relative to the reversing effect of HT.

GSH-Induced l-DES Activity and H 2 S Synthesis Reduces Methylglyoxal Content and Upregulates Glyoxalase System Enzymes' Activities in Pepper under SS
Relative to the control plants, SS dramatically caused increased accumulation of methylglyoxal (MG) by 2.52-fold. However, the treatments of GSH and GSH+NaHS exhibited 23.7% and 36.3% less generation of MG, respectively, in the SS-plants over the controls ( Figure 10A).
Salinity stress increased the activity of Gly I by 71.1%, but decreased that of Gly II by 51.1% over the controls (Figure 10B,C). Both GSH and GSH+NaHS supplemented to the SS-plants led to increases in the activity of Gly I by 73.4% and 98.1%, and that of Gly II by 103.1% and 137.2%, respectively relative to those in the SS-plants alone.
The positive effects of GSH on MG, Gly I, and Gly II were totally reversed by the supply of PAG and HT, as it was evidently observed that PAG or HT increased MG and reduced the activities of Gly I and Gly II. However, when NaHS was externally applied along with GSH (GSH+NaHS), HT eliminated the positive effect of GSH+NaHS on MG, Gly I, and Gly II by reducing the H 2 S content without significantly reducing the l-DES enzyme activity. PAG did not significantly reverse the positive effect of GSH+NaHS on the reduced content of MG; however, it increased the activities of both Gly I and Gly II, coupled with the reduced activity of l-DES.

SA-Induced Enhancement in Phenotypic Appearance of the Pepper Plants
As seen in Figure 11, the phenotypic appearance of the pepper plants showed that salt stress (SS) led to chlorosis, exhibiting yellow spots on the leaves. Salinity also caused reduced plant height and leaf size. Supplementation of GSH or GSH+NaHS eliminated such symptoms induced by SS. However, supplementation of the inhibitor of l-DES, PAG, or the scavenger of H 2 S, hypotaurine (HT), reversed the positive effect of GSH on the phenotypic appearance of plant leaves.

GSH-Induced L-DES Activity and H2S Synthesis Reduces Methylglyoxal Content and Upregulates Glyoxalase System Enzymes' Activities in Pepper under SS
Relative to the control plants, SS dramatically caused increased accumulation of methylglyoxal (MG) by 2.52-fold. However, the treatments of GSH and GSH+NaHS exhibited 23.7% and 36.3% less generation of MG, respectively, in the SS-plants over the controls (Figure 10A). Salinity stress increased the activity of Gly I by 71.1%, but decreased that of Gly II by 51.1% over the controls (Figure 10B,C). Both GSH and GSH+NaHS supplemented to the SS-plants led to increases in the activity of Gly I by 73.4% and 98.1%, and that of Gly II by 103.1% and 137.2%, respectively relative to those in the SS-plants alone.
The positive effects of GSH on MG, Gly I, and Gly II were totally reversed by the supply of PAG and HT, as it was evidently observed that PAG or HT increased MG and reduced the activities of Gly I and Gly II. However, when NaHS was externally applied along with GSH (GSH+NaHS), HT eliminated the positive effect of GSH+NaHS on MG, Gly I, and Gly II by reducing the H2S content without significantly reducing the L-DES enzyme activity. PAG did not significantly reverse the positive effect of GSH+NaHS on the reduced content of MG; however, it increased the activities of both Gly I and Gly II, coupled with the reduced activity of L-DES.

SA-Induced Enhancement in Phenotypic Appearance of the Pepper Plants
As seen in Figure 11, the phenotypic appearance of the pepper plants showed that salt stress (SS) led to chlorosis, exhibiting yellow spots on the leaves. Salinity also caused reduced plant height and

Discussion
Reduced plant growth is one of the well-known detrimental effects induced by salt stress [69][70][71], as has been consistently observed in this study. The reduced plant growth due to SS could be due to low uptake of essential nutrient elements [72], e.g., potassium (K + ) [73] and calcium (Ca +2 ) [74], and Na + toxicity [75]. An analogous observation has been obtained in the present study, wherein salinity stress reduced plant growth, leaf K + , followed by high Na + accumulation. An alternatively assumed reason for reduced plant growth could be decreased water uptake in plants because of osmotic stress induced by salinity [76,77]. Similarly, leaf water content (RWC) and water potential were reduced in SS plants in the present study.
Our results indicated that GSH partly alleviated the deleterious effect of SS on plant growth, suggesting that GSH might be an active compound participating in SS tolerance of sweet pepper plants, as has previously been observed in rice [78] and tomato [25]. The positive response of GSH has previously been reported on some plant species under various stresses [27], such as water stress [79], high-temperature stress in mung bean [28], and lead stress in wheat [29]. The alleviation effect of GSH on salt-induced inhibited plant growth might be associated with enhancements in leaf chlorophyll content, Fv/Fm, and K + content, which were initially depressed due to SS. Our results

Discussion
Reduced plant growth is one of the well-known detrimental effects induced by salt stress [69][70][71], as has been consistently observed in this study. The reduced plant growth due to SS could be due to low uptake of essential nutrient elements [72], e.g., potassium (K + ) [73] and calcium (Ca +2 ) [74], and Na + toxicity [75]. An analogous observation has been obtained in the present study, wherein salinity stress reduced plant growth, leaf K + , followed by high Na + accumulation. An alternatively assumed reason for reduced plant growth could be decreased water uptake in plants because of osmotic stress induced by salinity [76,77]. Similarly, leaf water content (RWC) and water potential were reduced in SS plants in the present study.
Our results indicated that GSH partly alleviated the deleterious effect of SS on plant growth, suggesting that GSH might be an active compound participating in SS tolerance of sweet pepper plants, as has previously been observed in rice [78] and tomato [25]. The positive response of GSH has previously been reported on some plant species under various stresses [27], such as water stress [79], high-temperature stress in mung bean [28], and lead stress in wheat [29]. The alleviation effect of GSH on salt-induced inhibited plant growth might be associated with enhancements in leaf chlorophyll content, F v /F m , and K + content, which were initially depressed due to SS. Our results suggest that GSH can possibly contribute to the plant response to salt stress, as has formerly been observed in cotton [80] and tomato [81].
It has previously been reported that l-DES is known as the key enzyme for endogenous H 2 S generation in plants converting cysteine into H 2 S [82]. Inhibition in the activity of l-DES and endogenous H 2 S synthesis using PAG or HT reversed the positive effect of GSH under SS. This suggests that l-DES and H 2 S are both involved in GSH-induced SS tolerance in the pepper plants. Furthermore, our results reveal that GSH activates the l-DES enzyme, which is the main producer of H 2 S involved in improving tolerance to salt stress. Hence, H 2 S might act as a downstream signal molecule of l-DES that is activated by GSH. Furthermore, to provide further evidence that H 2 S plays a role in GSH-induced SS tolerance, NaHS was supplemented externally along with GSH. The application of GSH+NaHS led to a further increase in plant growth, showing that H 2 S could be involved in GSH-induced SS tolerance. The alleviation effect of GSH+NaHS was completely reversed by HT, but not by PAG, under saline conditions, suggesting that PAG did not successfully reverse the endogenous H 2 S content, although it blocked the activity of the l-DES enzyme when NaHS was supplied externally. These findings indicate that both the l-DES enzyme and H 2 S are needed for making the external application of GSH effective in regulating salinity tolerance. It has been well-reviewed that endogenous H 2 S plays a key role in salinity stress tolerance [42]. Externally supplemented H 2 S has been observed to alleviate the harmful effects of salt stress in several plant species, e.g., wheat [83], rice [84], and cucumber [42], as well as other stresses, such as cadmium stress in rice [85], drought stress in spinach [86], and chromium stress in maize [87]. However, no study has been reported in the available literature on the role of l-DES activity and H 2 S in GSH-induced SS tolerance. Hereafter, this could be the first report uncovering the mechanism of GSH-induced tolerance to SS.
The detrimental effect of salt stress on chlorophyll content has been widely observed in common bean [88] and cucumber [89], as similarly observed in our study. The decrease in chlorophyll due to SS in the pepper plants might have been associated with the explicit appearance of oxidative stress due to SS [88]. Furthermore, chlorophyllase enzyme might have been involved in blocking or deprivation of chlorophyll synthesis [90]. Another photosynthetic parameter affected by salinity is the F v /F m , which was reduced in SS-plants, as previously observed in various plants, e.g., in alfalfa [91], parsley [92], and cucumber [93]. Reduced F v /F m due to salinity showed that the reaction center of PSII is impaired or deactivated, resulting in photoinhibition in the stressed plants [92]. The existence of oxidative damage due to SS was related to reduced F v /F m [94].
The GSH-induced improvement in photosynthetic traits and reduction in H 2 O 2 levels in the SS-plants reveal that GS participates in relieving the negative effects of SS on photosynthetic traits. These findings are consistent with those for other plant species, e.g., tomato [95] and mung bean [30]. GSH has also been shown to improve the synthesis of chlorophyll in salt-stressed rice plants [69].
It has been shown that GSH had a positive effect on endogenous l-DES activity and H 2 S synthesis in the salt-stressed pepper plants. However, pretreatment of PAG or HT disrupted the improvement in l-DES activity and H 2 S synthesis, and so both these pretreatments eliminated the improvement in chlorophyll content and F v /F m , indicating that both l-DES and H 2 S might have played a key function in GSH-enhanced chlorophyll content and F v /F m . Furthermore, the findings so obtained advocate that the role of the l-DES enzyme in GSH-induced improvement in salt tolerance might be due to the generation of H 2 S, which in turn participates in improving photosynthesis-related attributes. Analogous results have been obtained by other studies, exhibiting that externally applied H 2 S improved chlorophyll content in spinach under drought stress [86] and cucumber under salt stress [42].
The reduced leaf water status and increased proline and glycine betaine (GB) contents due to SS have been reported in various plants [96,97], similar to what has been observed in our study. The accumulation of proline under SS is known to be a mechanism responsible for stress tolerance of plants [98]. Glycine betaine is another osmoregulator that is actively involved in upregulating enzymes' activities and, in turn, sustaining the integrity of membranes against the harmful effects of SS [99]. Although both proline and GB were found to be elevated in the pepper plants under SS, their accumulation might not have been enough to alleviate the oxidative stress due to the overgeneration of H 2 O 2 in the present study. However, GSH led to further increases in proline and GB contents in the salt-stressed pepper plants. Herein, proline and GB accumulation could be crucial osmolytes in GSH-induced improvement in the leaf water status of plants under SS, as has been previously observed in mung bean by Nahar et al. [30].
The supplementation of PAG or HT reversed the beneficial effects of GSH on leaf water status and proline and GB under SS conditions, particularly by reducing the activity of l-DES and endogenous H 2 S content. This shows that both l-DES and H 2 S participate in GSH-induced improvement in leaf water status and proline and GB contents under SS. Furthermore, HT eliminated the positive effect of GSH+NAHS, but the reversing effect of PAG remained partial on the same parameters under SS. The previous reports show that exogenously applied H 2 S enhanced leaf water content in strawberry [100] and proline in rice [84] under salinity stress. These results show that endogenous H 2 S content is primarily needed in GSH-induced regulation of leaf water status and osmolytes (proline and GB), and l-DES is only needed to produce H 2 S when H 2 S is not supplied externally as NaHS.
In this study, l-DES activity and H 2 S synthesis increased in the SS-pepper plants, as have been reported earlier; salinity stress increased l-DES activity in alfalfa and tobacco [35,36]. Furthermore, l-DES activity and H 2 S synthesis were found to be further enhanced in the SS-plants treated with GSH and GSH+NaHS, showing that H 2 S acts as a signal molecule produced by l-DES, which is activated by GSH under SS conditions. This clearly shows that inhibition of l-DES activity by applying PAG causes a decrease in H 2 S synthesis/accumulation. The low concentration of H 2 S is thought to enhance the tolerance to abiotic and biotic stresses in plants, but high concentration can be detrimental to plant growth [101]. Hence, an acceptable level of H 2 S must be sustained in the plant cell to enhance the tolerance of plants to stress conditions. Therefore, in the current study, H 2 S induced by GSH was at an optimum level, which was not toxic to plants, providing evidence that no damaging effects on plant physiological processes were observed. Our results suggest that GSH might have activated the l-DES enzyme to synthesize H 2 S, but the beneficial effect of GSH was eliminated by supplementing PAG or HT through the reduction of l-DES activity and H 2 S content. So, the positive effect of GSH under SS could be due to the activation of H 2 S synthesis via increasing the activity of l-DES.
Salinity stress increased oxidative-stress-related parameters such as H 2 O 2 , MDA, and EL. This might have been due to the repressed activities of MDAR and DHAR, which are potential scavenger enzymes of H 2 O 2 , as similarly suggested by Yan et al. [81] under SS. The present observations also suggest that externally applied GSH is effective in inducing ROS detoxification in SS-pepper plants, and this could be one of the main strategies for GSH to relieve oxidative stress due to salinity and improve salinity tolerance. Such consistent observations were also reported in mung bean [30] and tomato [81]. Furthermore, NaHS applied along with GSH (GSH+NaHS) led to further reversal effects on oxidative stress parameters, showing that these two metabolites together have a stimulating effect on the reversal of oxidative stress. The pretreatment with PAG or HT had an eliminating effect on GSH-induced reduced oxidative stress under SS. This suggests that GSH might have triggered the l-DES activity, the potential source of H 2 S, and so H 2 S alleviating oxidative stress might have improved SS tolerance in the pepper plants. However, when GSH and NaHS were applied together (GSH+NaHS), the supply of HT completely reversed the positive effects of GSH+NaHS on oxidative stress, but that of PAG did not show a complete reversal effect. The reason for this could be that PAG did not eliminate the endogenous H 2 S, although it reversed the l-DES activity. In view of these data, it can be suggested that GSH-induced l-DES activity reverses oxidative stress under SS. However, for the maintenance of endogenous H 2 S at desirable levels by the supplementation of NaHS, GSH is not dependent on l-DES activity to reverse oxidative stress. The beneficial effect of H 2 S has previously been observed in different plant species, e.g., in maize [102], rice [84], and pepper [103].
The SOD is one of the antioxidant enzymes scavenging ROS by converting superoxide O 2 •− to H 2 O 2 at the first stage of the defense system [104]. Salinity stress increased the SOD activity, and such consistent results were also observed in mung bean [30], chickpea [105], and soybean [21].
Application of GSH led to a further increase in the activity of SOD, as similarly observed in mung bean [30]. More stimulating effects on the SOD activity was obtained by providing NaHS and GSH jointly (GSH+NaHS).
Salinity stress increased the activities of CAT and POD, as has been observed in wheat [106]. The treatment of GSH further increased the activities of CAT and POD under SS, as has been observed in canola [107], mung bean [30], and tomato [81]. However, the supply of PAG or HT eliminated the positive effect of GSH on SOD, CAT, and POD activities under SS, suggesting that GSH-induced enhanced activities of the antioxidant enzymes is dependent on the l-DES activity and endogenous H 2 S accumulation. Externally supplied NaHS, a donor of H 2 S, has been shown to improve the activities of antioxidant enzymes under saline stress [106,108]. Pretreatment with HT eliminated the beneficial effect of GSH on SOD, CAT, and POD activities under SS, but PAG did not reverse the action of GSH when GSH was supplied along with NaHS (GSH+NaHS).
Ascorbate acts as a scavenger of ROS by either directly scavenging ROS or helping the detoxification of H 2 O 2 via the AsA-GSH cycle [20,109]. Often, high AsA accumulation in plants helps plants to have a high tolerance to stress conditions [110]. In the present experiment, SS significantly decreased the AsA content due to the overaccumulation of H 2 O 2 or the inhibition of the synthesis or restoring synthesis of AsA under stress conditions [20]. However, GSH supplementation led to increased AsA content in the SS-seedlings, perhaps by improving MDHAR and DHAR activities. These results were also consistent with the results reported in mung bean and Brassica napus under SS [20,30].
Glutathione has been reported to alleviate oxidative stress by eliminating ROS and is involved in maintaining the cellular redox status [20]. So, this special function of GSH makes it favorable to SS-plants [30]. The content of GSH increased in the SS-pepper plants, which is not consistent with some previous reports [30,111]. GSH contributes to ROS scavenging and is converted into GSSG, thereby increasing GSSG levels under SS, as observed in the present study. However, the addition of GSH reduced GSSG and increased GSH levels, as already observed in mung bean [30] and tomato [31] plants under saline stress. The ratio of GSH/GSSG is also one of the key indicators for the assessment of stress tolerance of plants. It means that the higher the GSH/GSSG ratio, the higher the stress tolerance of plants [112], as observed in this study with the exogenous application of GSH and GSH+NaHS. Salinity stress and addition of GSH and GSH+NaHS increased the activity of GR, which, in turn, helped to restore GSH and the GSH/GSSG ratio of the pepper plants to some extent. This has also been reported in mung bean plants under saline stress [30].
The supplementation of PAG and HT reversed the beneficial effect of GSH on nonenzymatic antioxidant levels; this was achieved by reducing the content of l-DES and H 2 S with PAG and by reducing H 2 S without lowering the l-DES activity with HT. However, PAG has not been fully successful in reversing the positive effect of GSH+NaHS when NaHS was supplied along with GSH (GSH+NaHS), since PAG reduced the activity of l-DES but did not reduce the H 2 S content completely. The HT pretreatment fully eliminated the positive effect of GSH+NaHS by inhibiting the H 2 S content without reducing l-DES activity. These results suggest that the l-DES activity and synthesis of H 2 S are involved in GSH-induced upregulation of nonenzymatic antioxidants under saline stress. However, when NaHS was supplied externally as a source of H 2 S, the positive effect of GSH did not depend on the l-DES activity. It has been shown that exogenously applied H 2 S upregulated AsA and GSH in maize [102] and wheat [113] plants under SS.
In the initial stage of the AsA-GSH cycle, APX scavenges H 2 O 2 [114]. Previously, it has been described that APX can be positively modulated by H 2 S through persufidation [115]. Therefore, the increased activity of APX under saline stress is expected, as observed in this study. The supplementation of GSH and GSH+NaHS led to a further elevation in the activity of APX under SS, as consistently observed in mung bean and tomato plants under saline stress [30,81].
Another two enzymes related to the AsA-GSH cycle are the MDHAR and DHAR involved in AsA production [116]. In the present study, the SS-plants showed a decrease in MDHAR and DHAR activities, similar to what had been observed in Brassica napus by Hasanuzzaman et al. [20]. Nonetheless, the activities of DHAR and MDHAR were increased in the plants treated with GSH or GSH+NaHS, as consistently observed in tomato plants [107].
Glutathione reductase (GR) is also one of the key enzymes in the AsA-GSH cycle; it plays a crucial role in maintaining the pool or ratio of GSH/GSSG [98]. Furthermore, GR restores GSH from the oxidized GSSG using NADPH, which is generated predominantly from photosynthesis [27]. In this study, salt stress increased the activity of GR, as found in soybean and its activity was further elevated by the GSH and GSH+NaHS applications. Consistent results have also been reported in mung bean [30].
Pretreatment with PAG or HT eliminated the beneficial effect of GSH on the AsA-GSH-cycle-related enzymes by lowering H 2 S content. However, PAG has been partly effective in eliminating the positive effects of GSH+NaHS when NaHS was jointly supplied with GSH (GSH+NaHS). It is pertinent to note here that PAG did not completely reduce H 2 S content, but it completely reduced the activity of l-DES. The HT pretreatment fully eliminated the positive effect of GSH+NaHS by inhibiting the H 2 S content without reducing the l-DES activity. These data suggest that the l-DES activity and H 2 S play key functions in GSH-induced upregulation in the AsA-GSH-cycle-related enzymes under SS. Furthermore, the data show that the l-DES activity is not needed for making GSH, being effective for the upregulation of the AsA-GSH-cycle enzymes when NaHS was supplied externally as a source of H 2 S. The positive role of H 2 S on the AsA-GSH-cycle enzymes has previously been observed in plants under SS [102,113].
Plants require nutrient elements at an adequate rate to sustain their structure and key physiological processes [117]. However, these metabolic processes may be disturbed when such nutrients are not supplied sufficiently [118]. Salinity-induced high Na + accumulation may occur due to potassium (K + ) deficiency caused by the antagonistic effect of Na + for K + binding sites [119]. Therefore, an optimum K + /Na + ratio is the main attribute for the salinity tolerance of plants [120]. Salinity stress lowers the K + /Na + ratio because of the low level of K + over that of Na + in the leaves of plants [121]. However, GSH application lowered the leaf Na + and elevated leaf K + and the K + :Na + ratio in the plants under saline stress, as recently reported by Zhou et al. [25].
Pretreatment with PAG or HT, along with GSH, eliminated the positive effect of GSH on leaf K + and Na + contents, as well as leaf K + /Na + ratio, indicating that GSH promotes l-DES activity and H 2 S synthesis so as to enhance salt tolerance. The positive effect of externally applied GSH+NaHS was eliminated by HT by reducing the H 2 S content without reducing the l-DES activity, but it was not reversed by PAG, although PAG reduced the l-DES activity without reducing H 2 S. This shows that GSH-induced improvement in leaf K + and the K + /Na + ratio and reduction in Na + contents is dependent on H 2 S content, but not on l-DES activity when NaHS is supplied externally as a source of H 2 S. Earlier studies have consistently shown that exogenously applied H 2 S improved salinity tolerance by reducing Na + and increasing K and the K + /Na + ratio in different plants, e.g., alfalfa [35], rice [84], and cucumber [40].
The accumulation of MG enhances in plant cells under stress conditions and it may be a potentially toxic compound that damages several physiological processes [30]. The plants subjected to SS showed high MG accumulation, as was observed in mung bean [30], Brassica spp. [122], and tomato [10] under SS. The glyoxalase system consists of two enzymes (Gly I and Gly II) to detoxify the overgeneration of methylglyoxal (MG) by consuming GSH as a substrate and thereby causes enhanced stress tolerance [123,124]. So, increased GSH content may impart a positive effect on reducing MG-induced oxidative stress [27]. In this study, increased GSH content under SS due to GSH treatment reduced the MG accumulation, thereby reducing oxidative stress in the pepper plants. Another strategy to reduce the MG accumulation is to upregulate the glyoxalase-system-related enzymes, Gly I and Gly II, in many plant species under stress conditions [122]. In the present study, SS increased the activity of Gly I, but decreased that of Gly II in the pepper plants, similar to what was observed in Brassica napus [20], tomato [125], and soybean [21]. Pretreatment with GSH significantly enhanced both enzyme activities and reduced the MG content, suggesting a detoxification effect of MG and improvement in SS tolerance in the pepper plants. Similar observations have also been reported in mung bean [30].
Pretreatment with PAG or HT eliminated the up-regulation effect of GSH on the glyoxalase system and positive effect of MG detoxification by inhibiting the activity of l-DES or synthesis of H 2 S in the pepper plants under SS. This shows that the beneficial effect of GSH is dependent on l-DES activity or H 2 S content. The beneficial effect of exogenously applied H 2 S in upregulating the glyoxalase system has been earlier reported in rice [78]. The benefit of externally applied GSH+NaHS was reversed by HT by lowering H 2 S without altering l-DES activity. This shows that GSH-induced improvement in the glyoxalase system and the reduction in MG accumulation need H 2 S alone rather than the l-DES activity when NaHS is supplied externally as a source of H 2 S.

Conclusions
As explained earlier, the suggested mechanism of GSH-induced SS tolerance in pepper plants is by increasing H 2 S due to the activation of the l-DES enzyme, which is the main producer of H 2 S. In this way, it alleviates the oxidative stress by stimulating the AsA-GSH-cycle-related enzymes and the glyoxalase system, as well as by detoxifying the MG content, which, in turn, reduces Na + content and increases K + content. Additional proof shows that HT or PAG eliminates SS tolerance by reducing either H 2 S content or l-DES activity, and downregulating the AsA-GSH cycle and glyoxalase system, as well as eliminating the GSH-induced decrease in oxidative stress and MG content, thereby again enhancing Na + and reducing K + content in plants.
Upcoming investigation in this area under field conditions might contribute to sustainable crop production, particularly in soils contaminated with high salinity. Furthermore, the participation of other signaling molecules and enzymes in the GSH-induced response of plants under salt stress and other stressors also needs to be investigated.

Conflicts of Interest:
The authors declare no conflict of interest.