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Placenta-Derived Mesenchymal Stem Cells Restore the Ovary Function in an Ovariectomized Rat Model via an Antioxidant Effect

1
Department of Biomedical Science, CHA University, Seongnam 13488, Korea
2
Department of Oral Pathology, Gangneung-Wonju National University, Gangneung 26403, Korea
3
Department of Integrated Biomedical and Life Sciences, College of Health Science, Korea University, Seoul 02841, Korea
4
Department of Convergence Medicine, School of Medicine, University of Ulsan and Asan Medical Center, Seoul 05505, Korea
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(7), 591; https://doi.org/10.3390/antiox9070591
Received: 12 June 2020 / Revised: 1 July 2020 / Accepted: 3 July 2020 / Published: 6 July 2020
(This article belongs to the Section Health Outcomes of Antioxidants and Oxidative Stress)
Oxidative stress is one of the major etiologies of ovarian dysfunction, including premature ovarian failure (POF). Previous reports have demonstrated the therapeutic effects of human placenta-derived mesenchymal stem cells (PD-MSCs) in an ovariectomized rat model (OVX). However, their therapeutic mechanism in oxidative stress has not been reported. Therefore, we investigated to profile the exosome of serum and demonstrate the therapeutic effect of PD-MSCs transplantation for the ovary function. We established an OVX model by ovariectomy and PD-MSCs transplantation was conducted by intravenous injection. Additionally, various factors in the exosome were profiled by LC-MS analysis. As a result, the transplanted PD-MSCs were engrafted into the ovary and the existence of antioxidant factors in the exosome. A decreased expression of oxidative stress markers and increased expression of antioxidant markers were shown in the transplantation (Tx) in comparison to the non-transplantation group (NTx) (* p < 0.05). The apoptosis factors were decreased, and ovary function was improved in Tx in comparison to NTx (* p < 0.05). These results suggest that transplanted PD-MSCs restore the ovarian function in an OVX model via upregulated antioxidant factors. These findings offer new insights for further understanding of stem cell therapy for reproductive systems. View Full-Text
Keywords: premature ovarian failure; ovariectomized rat model; stem cell therapy; placenta-derived mesenchymal stem cells; folliculogenesis; reactive oxidative stress; antioxidants premature ovarian failure; ovariectomized rat model; stem cell therapy; placenta-derived mesenchymal stem cells; folliculogenesis; reactive oxidative stress; antioxidants
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MDPI and ACS Style

Seok, J.; Park, H.; Choi, J.H.; Lim, J.-Y.; Kim, K.G.; Kim, G.J. Placenta-Derived Mesenchymal Stem Cells Restore the Ovary Function in an Ovariectomized Rat Model via an Antioxidant Effect. Antioxidants 2020, 9, 591. https://doi.org/10.3390/antiox9070591

AMA Style

Seok J, Park H, Choi JH, Lim J-Y, Kim KG, Kim GJ. Placenta-Derived Mesenchymal Stem Cells Restore the Ovary Function in an Ovariectomized Rat Model via an Antioxidant Effect. Antioxidants. 2020; 9(7):591. https://doi.org/10.3390/antiox9070591

Chicago/Turabian Style

Seok, Jin, Hyeri Park, Jong H. Choi, Ja-Yun Lim, Kyung G. Kim, and Gi J. Kim. 2020. "Placenta-Derived Mesenchymal Stem Cells Restore the Ovary Function in an Ovariectomized Rat Model via an Antioxidant Effect" Antioxidants 9, no. 7: 591. https://doi.org/10.3390/antiox9070591

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