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Open AccessArticle

Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis

1
Department of Biochemistry and Molecular Biology, University of Bucharest, 91–95 Spl. Independentei, 050095 Bucharest, Romania
2
Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania
3
Faculty of Pharmacy, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania
4
SC PlantExtrakt SRL, 407059 Radaia, Cluj, Romania
5
Department of Histology, Faculty of Medicine, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania
*
Author to whom correspondence should be addressed.
These authors contributed equally to the manuscript.
Antioxidants 2019, 8(9), 416; https://doi.org/10.3390/antiox8090416
Received: 25 August 2019 / Revised: 10 September 2019 / Accepted: 17 September 2019 / Published: 19 September 2019
(This article belongs to the Special Issue Oxidative Stress in Food Additives and Other Exposomes)
Inflammatory bowel disease (IBD) represents a group of chronic autoimmune and idiopathic disorders that are characteristic of industrialized countries. In contrast to drug therapies, which exert several side effects, herbal remedies have constantly attracted the attention of researchers. Therefore, in the present study, a mother tincture (MT) from fresh, young, non-woody Thuja occidentalis L. branches with leaves was obtained using distillation-based techniques. Further, this was used to assess its in vitro and in vivo antioxidant activities and anti-inflammatory properties, and to validate it as a potential phytotherapeutic treatment for IBD. The characterization of the tincture included common phytochemical screening assays for antioxidant capacity measurement, cell viability assays on Caco-2 colon cells, and in vivo assessment of antioxidant and anti-inflammatory effects by histopathological and ultrastructural analysis of the intestinal mucosa, measurement of reduced glutathione, lipid peroxidation, and gene expression of the inflammation markers (interleukin-6 and tumor necrosis factor-α) in intestine after oral administration to an experimental mouse model of colon inflammation (colitis) developed by intrarectal administration of 2,4,6-trinitrobenzenesulfonic acid (TNBS). Our study proved that administration of 25 or 50 mg T. occidentalis MT/kg of body weight/day by gavage for 7 days succeeded in inhibiting the inflammatory process induced by TNBS in the intestine, most probably because of its rich contents of flavonoids and phenolic compounds. These data could contribute to the formulation of therapeutic products based on T. occidentalis that could come to the aid of IBD patients. View Full-Text
Keywords: Thuja occidentalis; ulcerative colitis; TNBS; antioxidants; inflammation; polyphenols Thuja occidentalis; ulcerative colitis; TNBS; antioxidants; inflammation; polyphenols
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Stan, M.S.; Voicu, S.N.; Caruntu, S.; Nica, I.C.; Olah, N.-K.; Burtescu, R.; Balta, C.; Rosu, M.; Herman, H.; Hermenean, A.; Dinischiotu, A. Antioxidant and Anti-Inflammatory Properties of a Thuja occidentalis Mother Tincture for the Treatment of Ulcerative Colitis. Antioxidants 2019, 8, 416.

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