Polyphenol Composition of Extracts of the Fruits of Laserpitium Krapffii Crantz and Their Antioxidant and Cytotoxic Activity

During inflammation, reactive oxygen species (ROS) are produced in large amounts, causing oxidative stress. Several studies confirm that plant extracts rich in phenolic compounds may inhibit ROS production. For that reason, the aim of this work is the qualitative and quantitative analysis of phenolic acids and flavonoids in the etheric (LAEN) and methanolic (LAM) extracts of the fruits of Laserpitium krapffii Crantz, as well as their antioxidative and cytotoxic properties. Liquid chromatography–electrospray tandem mass spectroscopy (LC-ESI-MS/MS) enabled the identification of 12 phenolic acids and nine flavonoids. Both tested extracts scavenged ROS in a concentration-dependent manner. Stronger activity was observed for the methanolic extract. The cytotoxic effect of both extracts in increasing concentrations on five types of cancer cell lines was also investigated. The cytotoxicity was estimated using trypan blue vital staining. It was found that the analyzed extracts induced the apoptosis of the cells of all the tested cell lines. In conclusion, our results present that the fruits of L. krapffii can be a source of valuable compounds with protective effects against oxidative damage.


Introduction
Laserpitium krapffii Crantz (syn. L. alpinum Waldst. and Kit.) is a perennial herbaceous plant that is commonly known as Krapf's laserwort. The species, belonging to the Apiaceae family, is widely spread throughout the northern regions of the Balkan Peninsula, and the southern and eastern parts of the Alps [1].
Reports regarding the chemical composition and biological activities of L. krapffii are limited. Primarily data indicated the content of only sesquiterpene lactones [2,3] and essential oils [4] in this plant.

Extraction Method
First, 50.0 g of dried and powdered fruits were extracted successively in the filter paper with petroleum ether (bp 45-60 • C; 300 mL) for over 40 h in a Soxhlet apparatus. Etheric extract was filtered and evaporated under reduced pressure, and then subjected to lyophilization using a vacuum concentrator (Free Zone 1 apparatus; Labconco, Kansas City, KS, USA) affording 4.2 g of dried fraction (LAEN). In the next stage, dried fruits (after petroleum ether extraction) were sonicated with a mixture of methanol-acetone-water (3:1:1, v/v/v) (3 × 100 mL) at a controlled temperature (40 ± 2 • C) for 45 min. Combined methanolic extracts were filtered, concentrated under reduced pressure, and lyophilized in a vacuum concentrator to obtain 11.3 g of dried residue (LAM).

Solid Phase Extraction (SPE)
Both crude extracts (LAEN, LAM) were purified using an Solid Phase Extraction (SPE) system (Baker spe-12G™, J.T. Baker, Germany) and previously conditioned Bakerbond C-18 column (Octadecyl, 500 mg, J.T. Baker, Phillipsburg, USA). Then, 5 mL of extracts in 30% methanolic were eluted with 5 mL of water, followed by 5 mL of 50% and finally 5 mL of 80% methanol. The obtained extracts were evaporated to dryness and used for LC-MS/MS analysis.

Total Phenolic, Flavonoid, and Phenolic Acids Content
The total phenolic (TPC) and total flavonoid content (TFC) were determined using the colorimetric methods previously described [20]. The absorbance was measured at 680 nm and 430 nm, respectively, using an ELISA Reader. The total phenolic concentration was calculated from the calibrated curve (R 2 = 0.9977), using gallic acid in a concentration of 0.001 to 0.008 mg/mL as a standard. The results were expressed as mg of gallic acid equivalent (GAE) per 1 g of dry extract (DE). TFC was calculated from the calibrated curve (R 2 = 0.9999), using quercetin in a concentration of 0.05 to 0.20 mg/mL as a standard. The results were expressed as mg of quercetin equivalent (QE) per 1 g of DE. The total phenolic acids content (TPAC) was performed using the method with Arnov's reagent, as described in Polish Pharmacopoeia IX [19]. The absorbance was measured at 490 nm. The total phenolic acids content was calculated from the calibrated curve (R 2 = 0.9964), using caffeic acid in a concentration of 3.36 to 23.52 µg/mL. The results were expressed as mg of caffeic acid equivalent (CAE) per 1 g of DE.
MS detection was made in a 3200 QTRAP Mass spectrometer (AB Sciex, Framingham, MA, USA) with an electrospray ionization source (ESI) and a triple quadrupole-ion trap mass analyzer that was monitored by the Analyst 1.5 software. The ESI worked in the negative-ion mode, and the optimum values of the source parameters were: capillary temperature 450 • C, curtain gas 30 psi, nebulizer gas 50 psi, source voltage −4500 V for the phenolic acids and flavonoid glycosides, and capillary temperature 550 • C, curtain gas 20 psi, nebulizer gas 30 psi, and source voltage −4500 V for the analysis of flavonoid aglycones. Nitrogen was applied as collision and curtain gas. For each compound, the optimum conditions of multiple reaction mode (MRM) were established in the infusion mode. The analytes were identified by comparing the retention time and m/z values obtained by MS and MS 2 with the mass spectra from corresponding references checked under the same conditions. The calibration curves from MRM mode were used for quantification of all samples. The identified compounds were quantified on the basis of their peak areas, and comparison with a calibration curve was obtained with the corresponding standards. Linearity ranges for the calibration curves were determined. The limits of detection (LOD) and quantification (LOQ) for all the samples were specified at signal-to-noise ratios of 3:1 and 10:1, respectively, using the injection of a series of dilute solutions with known concentrations [21]. Details of the conditions of LC-ESI-MS/MS analysis are shown in Tables 1 and 2.

Analysis of Cell Viability
The cells of all the lines were put on 12-well plates (Sarstedt GesmbH, Wiener Neudorf, Austria) at an initial density of 1 × 10 6 cells/mL. After incubation (24 h at 37 • C), the cell suspension was stimulated with investigated extracts at concentrations ranging from 1 to 1000 µg/mL for etheric extracts and from 1 to 5000 µg/mL for methanolic extracts. Then, 1 mL of cell suspension was centrifuged at 900 rpm for 5 min, the supernatant was discarded, and the cells were resuspended in 50 µL of PBS. Then, 10 µL of cell suspension was taken and mixed with 10 µL of 0.4% solution of Trypan blue reagent (Bio-Rad, Hercules, CA, USA). The samples were incubated for 5 min. Cell viability was measured using TC 10™ Automated Cell Counter (Bio-Rad). The experiment was done in triplicate. The IC 50 (half-maximal inhibitory concentration, the inhibitor concentration when cell viability is 50%) values of both extracts were determined using MS Excel.
The hypochlorous acid (HOCl)-scavenging effect of the extracts and the reference compound ascorbic acid was studied by means of 5-thio-2-nitrobenzoic acid (TNB) as a reductant oxidized by HOCl into 5,5-dithiobis (2-nitrobenzoic acid) according to the method described earlier by Czerwińska et al. [26]. The absorbance was monitored at 412 nm. The scavenging of superoxide anion (O 2 •− ) was determined by means of a xanthine-xanthine oxidase system with the NBT reduction assay according to the previously reported procedure of Kiss et al. [27]. Quercetin was used as a positive control.
To estimate the samples influenced on the O 2 •− production by direct interaction with xanthine oxidase, enzyme activity was made by monitoring the uric acid formation at 295 nm [28].
All the results were expressed as the IC 50 values of the extracts on grounds on concentration-inhibition curves.

Statistical Analysis
All the results were expressed as means ± standard error of the mean (SEM) of three independent experiments. One-way ANOVA with Tukey's post hoc test was used for the statistical analysis of significance of differences between means. P values below 0.05 were accepted as statistically significant. All the investigations were done by means of Statistica 10.0 (StatSoft Poland, Cracow, Poland).

Polyphenol Composition
There are only a few reports in the literature about the composition and biological activity of Laserpitium species [2][3][4][5][9][10][11][12][14][15][16][17][18]. To date, the polyphenol composition of fruits of L. krapffii have not been investigated. Therefore, the first stage of our study was to examine the total content of these compounds in the etheric and methanolic extracts of fruits of L. krapffii. The amounts of phenolic compounds in the tested extracts were proportional to the intensity of absorption measured spectrophotometrically. The analysis indicated that the methanolic extract contains much larger quantities of total phenolic, flavonoids, and phenolic acids content. The obtained results are shown in Table 3.  The next purpose of our study was the qualitative and quantitative analysis of phenolic acids and flavonoids in obtained extracts. The optimized LC-ESI-MS/MS procedure allowed the identification of 12 phenolic acids and nine flavonoids in the etheric and methanolic extracts from the fruits of L. krapffii. The chromatograms of the flavonoid aglycones and glycosides in methanolic extract are shown in Figures 1 and 2, respectively. The amounts of all the compounds, which were quantified by a comparison of peak areas with the calibration curves of the corresponding references, are shown in Table 4. Both extracts did not differ significantly in the composition of polyphenols except for the absence of 3-hydroxybenzoic acid in the etheric extract. Gallic acid, eriodictyol, kaempferol, and nicotiflorin were identified in quantifiable amounts only in the methanolic extract.
Among the flavonoid aglycones, a large amount of quercetin (13.92 ± 0.03 µg/g of dry LAEN extract and 22.18 ± 0.09 µg/g of dry LAM extract) was found. The highest yields of flavonoid glycosides was obtained for the methanolic extract. Isoquercetin, nicotiflorin, and astragalin were presented in the greatest quantities in this extract.
To the best of our knowledge, L. krapffii was investigated for the first time in terms of phenolic acids and flavonoids quality and quantity. However, from the leaves of L. latifolium, astragalin, avicularin, isoquercitrin, quercitrin, rutin, chlorogenic and neochlorogenic acid, and other unidentified quercetin, isorhamnetin, and kaempferol derivatives were previously isolated [9].   Since plant phenolic acids and flavonoids have been noticed to possess a wide spectrum of biological activities including anti-inflammatory, antimutagenic, antioxidant, antitumor, and anticarcinogenic properties [29][30][31], the antioxidant and cytotoxic activity of the etheric and methanolic extracts obtained from the fruits of L. krapffii were also evaluated.

In Vitro Cytotoxicity Assay
The process of induction of neoplastic changes is associated with the appearance and accumulation of numerous mutations in the genetic material that caused the acquisition of unlimited division potential by phenotype cells. Such cells start to divide without limitations and lose their ability to die, creating a cancer. One way to eliminate cancer cells is to kill them by starting the process of apoptosis. The occurrence of multidrug resistance, inadequate efficacy, and numerous  Table 4. Content of phenolic acids and flavonoid aglycones and glycosides in the etheric (LAEN) and methanolic (LAM) extracts from L. krapffii fruits. Values are presented in means ± SEM, n = 3. nd-not detected; BQL-peak detected, concentration lower than the LOQ but higher than the LOD.

Compound LAEN LAM
Phenolic Acid/Flavonoid (µg per g of Dry Extract) Since plant phenolic acids and flavonoids have been noticed to possess a wide spectrum of biological activities including anti-inflammatory, antimutagenic, antioxidant, antitumor, and anticarcinogenic properties [29][30][31], the antioxidant and cytotoxic activity of the etheric and methanolic extracts obtained from the fruits of L. krapffii were also evaluated.

In Vitro Cytotoxicity Assay
The process of induction of neoplastic changes is associated with the appearance and accumulation of numerous mutations in the genetic material that caused the acquisition of unlimited division potential by phenotype cells. Such cells start to divide without limitations and lose their ability to die, creating a cancer. One way to eliminate cancer cells is to kill them by starting the process of apoptosis. The occurrence of multidrug resistance, inadequate efficacy, and numerous complications of current therapies motivate the search for new drugs. Plants are one of the basic sources of anti-cancer compounds [22,32].
In our report, the effect of the etheric and methanolic extracts in increasing concentrations on five types of cancer cell lines, HL-60 (Figure 3), HL-60/MX1 (Figure 4), HL-60/MX2 ( Figure 5), CEM/C1 (Figure 6), and CCRF/CEM (Figure 7) was investigated. The cytotoxicity was estimated using trypan blue vital staining. The cells of all the cancer lines exposed to the examined samples showed various cytotoxicity depending on the IC 50 value.
Based on the obtained results, it was found that the analyzed extracts from the fruits of L. krapffii induce apoptosis of the cells of all the tested cell lines. The results, which are given in Table 5, showed that the etheric extract (LAEN) significantly inhibited HL-60/MX1 human leukemia cells, and was the most potent sample with an IC 50 value of 31.08 µg/mL. Moreover, this extract showed a moderate cytotoxicity against HL-60 with IC 50 = 72.19 µg/mL and against CEM/C1 with IC 50 = 99.40 µg/mL. The etheric extract showed also relatively high cytotoxicity against CCRF/CEM (IC 50 = 172.58 µg/mL).
The weak cytotoxic activity was found in the methanolic extract from the fruits of L. krapffii. The IC 50 values for the LAM extract ranged from 205.72 µg/mL for the HL-60/MX1 line to 2848.31 µg/mL for the HL-60/MX2 line.
The presence of the large amounts of protocatechuic acid and quercetin may be responsible for the cytotoxic activity of the studied extracts. It has been reported that protocatechuic acid induces apoptosis in HL-60 human leukemia cells [33].
The published reports on the cytotoxic and antitumor activities of Laserpitium species are limited, and they focus on only a few species: Laserpitium latifolium L., L. zernyi Hayek, and L. ochridanum Micevski. The cytotoxic activities of chloroform extracts of dried roots and rhizomes of L. zernyi and L. ochridanum were tested against MCF 7/6 and MCF 7/AZ (human breast cancer) cell lines. The extracts exerted cytotoxic activities with the IC 50 values ranging from 65.21 to 348.25 µg/mL. The obtained results showed that the extract of L. ochridanum was most potent in the MTT test with IC 50 values of 65.21 µg/mL and 66.09 µg/mL in the MCF 7/AZ and MCF 7/6 cell lines, respectively [5].
Despite the use of different Laserpitium species and various cell lines as well as different research assays, the IC 50 values obtained in our work and previous works are similar.

Antioxidant Activity
In our research, the antioxidant activity of etheric and methanolic extracts of the fruits of L. krapffii was determined for the first time. We used four colorimetric methods to determine the activity of both tested extracts against synthetic radicals DPPH • and ABTS •+ , xanthine/xanthine oxidase (generating O2 •− ), as well as against HOCl. The measurement of antioxidant activities was performed on a microplate scale in cell-free systems.
The extracts examined in the study exhibited high scavenging ability in a concentrationdependent manner (Figure 8). For comparison, the radical scavenging activity of ascorbic acid was measured in the same conditions. The higher DPPH • scavenging activity was demonstrated for the methanolic extract (LAM). The IC50 value for methanolic extract was almost one and half times lower than the for ascorbic acid ( Table 6).
The percentage of ABTS •+ radicals reduction for extracts was measured at λ = 734 nm after 10 min of incubation (experimentally determined). In this time, both the analyzed extracts and the reference compound reached a constant antioxidant value. In comparison to gallic acid, which is a strong antioxidant compound [34], both tested extracts of L. krapffii showed weak antioxidant

Antioxidant Activity
In our research, the antioxidant activity of etheric and methanolic extracts of the fruits of L. krapffii was determined for the first time. We used four colorimetric methods to determine the activity of both tested extracts against synthetic radicals DPPH • and ABTS •+ , xanthine/xanthine oxidase (generating O 2 •− ), as well as against HOCl. The measurement of antioxidant activities was performed on a microplate scale in cell-free systems.
The extracts examined in the study exhibited high scavenging ability in a concentration-dependent manner (Figure 8). For comparison, the radical scavenging activity of ascorbic acid was measured in the same conditions. The higher DPPH • scavenging activity was demonstrated for the methanolic extract (LAM). The IC 50 value for methanolic extract was almost one and half times lower than the for ascorbic acid ( Table 6).
The percentage of ABTS •+ radicals reduction for extracts was measured at λ = 734 nm after 10 min of incubation (experimentally determined). In this time, both the analyzed extracts and the reference compound reached a constant antioxidant value. In comparison to gallic acid, which is a strong antioxidant compound [34], both tested extracts of L. krapffii showed weak antioxidant properties. The IC 50 values for the methanolic (20.69 µg/mL) and etheric (30.45 µg/mL) extracts were much higher than that for gallic acid, which was used as a positive control (0.75 µg/mL). In the study, we also evaluated the ability of the etheric and methanolic extracts of fruits of L. krapffii to scavenge O2 •− generated by the xanthine/xanthine oxidase system. Both extracts scavenge O2 •− in a concentration-dependent manner ( Figure 9A). At all the concentrations studied, LAM showed statistically (p < 0.05) stronger activity than the etheric extract did. The IC50 value for the methanolic extract (3.28 μg/mL) was almost five times lower than that for the etheric extract (17.31 μg/mL), and almost two times lower in comparison to the quercetin (5.72 μg/mL). Additionally, both extracts strongly inhibited uric acid production (for LAEN-97.23-26.41%, and for LAM-95.56-19.06%), confirming that their activity in a xanthine/xanthine oxidase system is related to the scavenging effect against O2 •− and the inhibition of the enzyme activity ( Figure 9B,C). In the study, we also evaluated the ability of the etheric and methanolic extracts of fruits of L. krapffii to scavenge O 2 •− generated by the xanthine/xanthine oxidase system. Both extracts scavenge O 2 •− in a concentration-dependent manner ( Figure 9A). At all the concentrations studied, LAM showed statistically (p < 0.05) stronger activity than the etheric extract did. The IC 50 value for the methanolic extract (3.28 µg/mL) was almost five times lower than that for the etheric extract (17.31 µg/mL), and almost two times lower in comparison to the quercetin (5.72 µg/mL). Additionally, both extracts strongly inhibited uric acid production (for LAEN-97.23-26.41%, and for LAM-95.56-19.06%), confirming that their activity in a xanthine/xanthine oxidase system is related to the scavenging effect against O 2 •− and the inhibition of the enzyme activity ( Figure 9B,C). The measurement of activity against HOCl ( Figure 10) showed that the extracts tested have a high scavenging ability of this radical. In the concentrations studied (2-50 µg/mL), the methanolic extract had stronger ability (17.91-80.04%) than the etheric one (2.45-61.23%), and the IC 50 values were 25.09 µg/mL and 38.17 µg/mL, respectively. Moreover, the IC 50 values for both extracts were lower than that for ascorbic acid, which was used as a reference compound (49.56 µg/mL). The measurement of activity against HOCl ( Figure 10) showed that the extracts tested have a high scavenging ability of this radical. In the concentrations studied (2-50 μg/mL), the methanolic extract had stronger ability (17.91-80.04%) than the etheric one (2.45-61.23%), and the IC50 values were 25.09 μg/mL and 38.17 μg/mL, respectively. Moreover, the IC50 values for both extracts were lower than that for ascorbic acid, which was used as a reference compound (49.56 μg/mL).  The obtained lower IC50 values for the methanolic extract in all the antioxidant assays may be connected with higher phenolic compounds content, as it is well known that flavonoids and phenolic acids are the most popular natural components responsible for the antioxidant activity of plants.

Conclusions
In the literature, there are no reports concerning the biological activities of extracts of Laserpitium krapffii. In this study, it was found that both extracts of the fruits of L. krapffii have high cytotoxic and antioxidant potential, and can be a source of valuable compounds with protective effects against oxidative damage. The inhibiting influence of both extracts on ROS production may reduce oxidative modifications of biomolecules and tissue dysfunctions during chronic degenerative diseases. . Data are expressed as means ± SEM, obtained in at least three independent experiments, and assayed in triplicate. Statistical significance of differences was calculated between both extracts; * p < 0.05. The obtained lower IC 50 values for the methanolic extract in all the antioxidant assays may be connected with higher phenolic compounds content, as it is well known that flavonoids and phenolic acids are the most popular natural components responsible for the antioxidant activity of plants.

Conclusions
In the literature, there are no reports concerning the biological activities of extracts of Laserpitium krapffii. In this study, it was found that both extracts of the fruits of L. krapffii have high cytotoxic and antioxidant potential, and can be a source of valuable compounds with protective effects against oxidative damage. The inhibiting influence of both extracts on ROS production may reduce oxidative modifications of biomolecules and tissue dysfunctions during chronic degenerative diseases.

Conflicts of Interest:
The authors declare no conflict of interest.