White Peony (Fermented Camellia sinensis) Polyphenols Help Prevent Alcoholic Liver Injury via Antioxidation

White peony is a type of white tea (Camellia sinensis) rich in polyphenols. In this study, polyphenols were extracted from white peony. In vitro experiments showed that white peony polyphenols (WPPs) possess strong free radical scavenging capabilities toward 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Long-term alcohol gavage was used to induce alcoholic liver injury in mice, and relevant indices of liver injury were examined. WPPs effectively reduced the liver indices of mice with liver injury. The serum levels of aspartate aminotransferase (ATS), alanine aminotransferase (ALT), alkaline phosphatase (ALP), triglycerides (TG), total cholesterol (TC), blood urea nitrogen (BUN), nitric oxide (NO), and malondialdehyde (MDA) were downregulated, while those of albumin (ALB), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were upregulated. WPPs also reduced the serum levels of interluekin-6 (IL-6), interluekin-12 (IL-12), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) in mice with liver injury. Pathology results showed that WPPs reduced alcohol-induced liver cell damage. Quantitative polymerase chain reaction (qPCR) and western blot results revealed that WPPs upregulated the mRNA and protein expressions of neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), manganese superoxide dismutase (Mn-SOD), cupro–zinc superoxide dismutase (Cu/Zn-SOD), and CAT and downregulated iNOS expression in the liver of mice with liver injury. WPPs protected against alcoholic liver injury, and this effect was equivalent to that of silymarin. High-performance liquid chromatography revealed that WPPs mainly contained the polyphenols gallic acid, catechinic acid, and hyperoside, which are critical for exerting preventive effects against alcoholic liver injury. Thus, WPPs are high-quality natural products with liver protective effects.


Introduction
White tea is a slightly fermented tea and is one of the six major teas in China. The raw material of white tea is the bud tip of the tea plant. White tea is light green and covered with frost. The tea soup is also lightly colored; thus, it is called white tea [1]. White peony is mainly produced in regions of Fujian Province, China. Its green leaves contain silver and white hearts in a flower shape. After brewing, the green leaves retain the buds and look like peony blossoms, which suggested the name white peony [2]. White tea possesses bioactivity. It cools the body, moistens the lungs, benefits the blood, has anti-inflammatory and detoxification effects, reduces blood pressure and fat, and decreases tiredness [3][4][5]. The polyphenols in white tea can protect the liver, accelerate the decomposition of the alcohol metabolite acetaldehyde, generate nontoxic substances, and reduce damage to liver cells. White tea can also affect the increased serum lactate dehydrogenase induced by hepatocellular injury, inhibit hepatic M cell collagen fiber formation, help protect the liver, greatly reduce alcohol damage to the liver, and rapidly restore the normal liver function [6].
Alcoholic liver disease is caused by long-term heavy drinking, manifests as fatty liver, and further develops into alcoholic hepatitis, liver fibrosis, and cirrhosis. Its main clinical features include nausea, vomiting, jaundice, hepatomegaly, and tenderness, combined with liver failure and upper gastrointestinal bleeding [7]. Severe drinking may induce extensive hepatocyte necrosis and even liver failure. Alcoholic hepatitis is a common liver disease that severely threatens patients' health. Alcoholic liver injury results from interactions between inflammatory reactions, oxidative stress, enterogenous endotoxins, and inflammatory mediators that are either directly or indirectly induced by alcohol and its derivatives during metabolic processes [8]. The main metabolic pathway of alcohol is in the liver and requires different subcellular organelles. Alcohol is metabolized by the enzyme alcohol dehydrogenase in the endoplasmic reticulum of the microsomal ethanol oxidation system and by aldehyde oxidase in the mitochondria [9]. The process generates reactive oxygen species (ROS), including superoxide anions, hydroxyl radicals, and hydrogen peroxide. ROS accumulation in the liver leads to functional disorders of the cell membrane, protein and DNA oxidation, and finally hepatocellular injury [10]. Initially, alcohol increases ROS via oxidative stress and induces fat accumulation in the liver. Under the effects of oxidative stress-related lipid peroxidation and inflammatory cytokines, hepatocytes with steatosis undergo a second attack, followed by inflammation, necrosis, and fibrosis [11].
Silymarin is a natural active substance extracted from the dried fruit of the plant silymarin and can protect liver cells from toxic substances, especially alcohol; at the same time, it has a strong antioxidant function, can protect liver cells from free radical damage, and its effect is far better than that of vitamin E; it can also promote the synthesis of proteins, accelerate the production of new liver cells, and repair liver cells [9]. Therefore, silymarin was used as a positive control in this study. White tea possesses antioxidant effects and enhances immunity. In this study, we investigated the effect of white peony (a type of white tea) containing polyphenols on alcoholic liver injury, examining its ability to regulate oxidative stress in mice. The polyphenols present in white peony were also identified to explain the mechanism of action of this tea against alcoholic liver injury.

Extraction of White Tea Polyphenols and Evaluation of the Antioxidant Activity in Vitro
One kilogram of freeze-dried white peony tea (Fuding Jinye Tea Industry Co., Ltd., Fujian, China) was weighed, ground, and divided into 10 samples. An alcohol solution (70% v/v) was added to each sample, and extraction was carried out for 4 h in a water bath at 70 • C, followed by filtration. The filtered solution was collected and refiltered through kieselguhr to remove fat-soluble impurities. The filtered solution was collected again and passed through a column of HP20 resin (Cool Chemical Technology Co., Ltd., Beijing, China) to adsorb the polyphenols present in broadleaf holly leaves. Subsequently, a 70% alcohol solution (v/v) was used to elute the resin and dissolve the adsorbed polyphenols on the resin. Finally, a white peony polyphenol (WPP) extract was obtained by evaporating the alcohol in a rotary evaporator. The scavenging capability of WPP on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals was detected in vitro following a previously described method [12].

Animal Experiment
Fifty 6-week-old male specific-pathogen-free Kunming mice (Experimental Animal Center of Chongqing Medical University, Chongqing, China) were acclimated for 1 week and assigned to normal, model, WPP low-concentration (WPPL), WPP high-concentration (WPPH), or silymarin groups (n = 10 per group). During the experiment, the mice were allowed to eat and drink freely for 8 weeks. The mice in the WPPL and WPPH groups were fed WPP at 50 mg/kg and 100 mg/kg per day, respectively. Those in the silymarin group were given silymarin by gavage at 100 mg/kg per day. All mice except those in the normal group received 50% alcohol (v/v) daily at 0.1 mL/10 g body weight. After 8 weeks, all mice were fasted for 24 h, then sacrificed. Blood was taken from the heart, and the livers were removed for subsequent experiments. The liver tissues were weighed, and the liver index was calculated per a previously established formula (liver index = liver weight/body weight × 100) [13]. The protocol for these experiments was approved by the Ethics Committee of Chongqing Collaborative Innovation Center for Functional Food (201807005B), Chongqing, China.

Histological Observations
The liver tissues (0.5 cm 2 ) were fixed in 10% formalin for 48 h, dehydrated, cleared in xylene, immersed in wax, embedded, sectioned, and stained with hematoxylin and eosin. The tissue morphology was observed under an optical microscope (BX43 microscope, Olympus, Tokyo, Japan).

RT-qPCR Analysis
The liver tissue was homogenized using a homogenizer, and the total RNA was extracted using TRIzol™ reagent (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The RNA was diluted to 1 µg/µL, then 1 µL of the total RNA solution was reverse-transcribed to obtain a cDNA template following the manufacturer's instructions (Thermo Fisher Scientific). qPCR was performed using SYBR Green PCR Master Mix (Thermo Fisher Scientific). The upstream and downstream primers used are shown in Table 1 (Thermo Fisher Scientific). qPCR was performed for 40 cycles using the following parameters: 95 • C for 60 s, followed by 95 • C for 15 s, 55 • C for 30 s, and 72 • C for 35 s (SteponePlus, Thermo Fisher Scientific). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal reference. The 2 −∆∆ Ct method was used to calculate relative gene expressions [13].

Western Blot
The liver tissue samples (100 mg) were homogenized with 1 mL RIPA (radio immunoprecipitation assay) buffer and 10 mL phenylmethylsulfonyl fluoride (PMSF) (Thermo Fisher Scientific) using a homogenizer at 12,000 r/min at 4 • C for 5 min. The homogenate was centrifuged at 12,000 r/min at 4 • C for 15 min. The middle protein layer was collected, and the proteins were quantitatively measured via the bicinchoninic acid (BCA) method. The samples were diluted at 50 µg/mL, mixed with sample buffer at a ratio of 4:1, heated at 100 • C for 5 min, and loaded into a gel. The separation gel and stacking gel were prepared using acrylamide, stacking buffer, distilled water, 10% ammonium persulfate (APS), and N,N,N',N'-tetramethylethylenediamine (TEMED) (Thermo Fisher Scientific) at a given ratio. A prestained protein ladder was also loaded into the gel. Vertical gel electrophoresis was performed by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) (Thermo Fisher Scientific) for 50 min. A polyvinylidene diflboride (PVDF) membrane was activated by methanol for 1 min, then incubated with a 1×TBST (Tris-Buffered saline Tween-20) solution containing 5% skim milk for 1 h after gel transfer. The PVDF membrane was then washed with 1×TBST and incubated with a primary antibody (Thermo Fisher Scientific) solution at 25 • C for 2 h. The PVDF membrane was further washed with 1×TBST 5 times and incubated with a secondary antibody (Thermo Fisher Scientific) solution at 25 • C for 1 h. Supersignal West Pico PLUS was spread on the PVDF membrane for observation via iBright FL1000 (Thermo Fisher Scientific) [13].

HPLC Analysis
Ten milligrams of the WPP sample was weighed, dissolved in 1 mL dimethyl sulfoxide (DMSO), and heated at 60 • C for 30 min in a water bath. The sample was then removed, and 1.5 mL of pure water and 1.5 mL of ethanol (high-performance-liquid-chromatography [HPLC] grade) were added. The sample was heated in a water bath at 60 • C for 10 min, centrifuged at 2000 rpm for 5 min, and filtered through a 0.22 µm filter membrane. The main components and WPP contents were determined via HPLC (UltiMate3000 HPLC System, Thermo Fisher Scientific). Mobile phase A was water containing 0.5% acetic acid, and mobile phase B was acetonitrile. The flow rate was 0.5 mL/min, with a column temperature of 30 • C, and the detection wavelength was 328 nm. The gradient elution conditions were as follows: equilibrium stage was set for 10 min with 12% B (isocratic), then 0-30 min

Statistical Analysis
All serum and tissues tests were performed in triplicate. The data were averaged and analyzed using Statistical Package for the Social Sciences (SPSS, Chicago, Illinois, IL, USA) software. One-way analysis of variance was used to analyze the data, and p < 0.05 was considered statistically significant.

Antioxidant Effects of WPPs in Vitro
As shown in Figure 1, the concentration of WPPs ranged from 0 to 50 µg, and the scavenging capacities of WPPs toward DPPH and ABTS increased linearly with their concentrations. With the increase of WPPs concentration, the scavenging effects on DPPH and ABTS radicals were enhanced, and these effects were slightly higher than those of vitamin C (ascorbic acid). It can be seen that WPP could scavenge DPPH and ABTS free radicals in vitro, and the effects were close to those of ascorbic acid, which is known to have good antioxidant properties.

Antioxidant Effects of WPPs in Vitro
As shown in Figure 1, the concentration of WPPs ranged from 0 to 50 μg, and the scavenging capacities of WPPs toward DPPH and ABTS increased linearly with their concentrations. With the increase of WPPs concentration, the scavenging effects on DPPH and ABTS radicals were enhanced, and these effects were slightly higher than those of vitamin C (ascorbic acid). It can be seen that WPP could scavenge DPPH and ABTS free radicals in vitro, and the effects were close to those of ascorbic acid, which is known to have good antioxidant properties.

Liver Index
During the experiment, the weight of the mice in each group increased normally, there was no significant difference (p > 0.05) between the weight of the mice in each group, and none of the mice died. (Figure 2). The liver index was the highest in the model group and the lowest in the normal group ( Table 2). The liver index of the model group was 2.15 times as high as that of the normal group; after WPP and silymarin treatment, the liver indexes decreased in mice with liver injury, and the effect of a high concentration of WPP (WPPH) was similar to that of silymarin (no significant difference between them, p > 0.05); the reduction of the liver index was significantly (p < 0.05) greater for WPPH than when administering low-concentration WPP (WPPL).

Liver Index
During the experiment, the weight of the mice in each group increased normally, there was no significant difference (p > 0.05) between the weight of the mice in each group, and none of the mice died. (Figure 2). The liver index was the highest in the model group and the lowest in the normal group ( Table 2). The liver index of the model group was 2.15 times as high as that of the normal group; after WPP and silymarin treatment, the liver indexes decreased in mice with liver injury, and the effect of a high concentration of WPP (WPPH) was similar to that of silymarin (no significant difference between them, p > 0.05); the reduction of the liver index was significantly (p < 0.05) greater for WPPH than when administering low-concentration WPP (WPPL).
died. (Figure 2). The liver index was the highest in the model group and the lowest in the normal group ( Table 2). The liver index of the model group was 2.15 times as high as that of the normal group; after WPP and silymarin treatment, the liver indexes decreased in mice with liver injury, and the effect of a high concentration of WPP (WPPH) was similar to that of silymarin (no significant difference between them, p > 0.05); the reduction of the liver index was significantly (p < 0.05) greater for WPPH than when administering low-concentration WPP (WPPL).  "±" for standard deviation (N = 10/group). a-d Using Tukey's honestly significant different test, there was no significant difference between the two groups with the same superscript (p > 0.05), and there was a significant difference between the two groups with different superscripts (p < 0.05). Silymarin: mouse treated with 100 mg/kg silymarin; WPPL: mouse treated with 50 mg/kg white peony polyphenols; WPPH: mouse treated with 100 mg/kg white peony polyphenols.

Liver Function-Related Serum Levels in Mice
The serum ALB levels in the model group were significantly (p < 0.05) lower than those in the normal group, but the levels of AST, ALT, ALP, TG, TC, and BUN were all significantly (p < 0.05) higher than those in the normal group (Table 3). WPPH reduced the serum levels of AST, ALT, ALP, TG, TC, and BUN and increased the ALB level in mice with liver injury. WPPH was about twice as effective as WPPL, and the effects of WPPH were similar to those of silymarin. "±" for standard deviation (N = 10/group). a-e Using Tukey's honestly significant different test, there was no significant difference between the two groups with the same superscript (p > 0.05), and there was a significant difference between the two groups with different superscript (p < 0.05). Silymarin: mouse treated with 100 mg/kg silymarin; WPPL: mouse treated with 50 mg/kg white peony polyphenols; WPPH: mouse treated with 100 mg/kg white peony polyphenols. ALT, alanine aminotransferase; AST, aspartate aminotransferase; ALP, alkaline phosphatase; TG, triglycerides; TC, total cholesterol; BUN, blood urea nitrogen; ALB, albumin.

Oxidation-Related Serum Levels in Mice
The SOD, CAT, and GSH-Px enzyme activities in the normal group were significantly higher than those in the other groups (p < 0.05), but NO and MDA levels were significantly lower than those in the other groups (p < 0.05; Table 4). In the model group, the serum enzymatic activities of SOD, CAT, and GSH-Px were the lowest, while NO and MDA levels were the highest. WPP effectively inhibited the liver injury-induced decreases in SOD, CAT, and GSH-Px activity and increased NO and MDA levels. Moreover, WPPH had a higher effect than WPPL, which was similar to that of silymarin.  "±" for standard deviation (N = 10/group). a-e Using Tukey's honestly significant different test, there was no significant difference between the two groups with the same superscript (p > 0.05), and there was a significant difference between the two groups with different superscript (p < 0.05). Silymarin: mouse treated with 100 mg/kg silymarin; WPPL: mouse treated with 50 mg/kg white peony polyphenols; WPPH: mouse treated with 100 mg/kg white peony polyphenols. SOD, superoxide dismutase; NO, nitric oxide; CAT, catalase; MDA, malondialdehyde; GSH-Px, glutathione peroxidase.

Cytokine Serum Levels in Mice
Interluekin-6(IL-6), IL-12, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) levels in the normal group were significantly lower than those in the other groups (p < 0.05), but those in the model group were significantly higher than those of the other groups (p < 0.05; Table 5). WPP effectively reduced the serum levels of IL-6, IL-12, TNF-α, and IFN-γ in mice with liver injury, and the effect of WPPH was similar to that of silymarin. "±" for standard deviation (N = 10/group). a-e Using Tukey's honestly significant different test, there was no significant difference between the two groups with the same superscript (p > 0.05), and there was a significant difference between the two groups with different superscript (p < 0.05). Silymarin: mouse treated with 100 mg/kg silymarin; WPPL: mouse treated with 50 mg/kg white peony polyphenols; WPPH: mouse treated with 100 mg/kg white peony polyphenols. IL-6, interluekin-6; TNF-α, tumor necrosis factor-alpha; IFN-γ; interferon-gamma.

Histological Analyses
Liver tissue cells in the normal group were normal in structure, and the hepatocytes were radially distributed around the central vein ( Figure 3). In the model group, the cells were nonuniformly arranged, the central veins were irregular, and the cell structure was destroyed, showing much cellular necrosis. Both WPP and silymarin alleviated alcohol-induced hepatocyte necrosis and incomplete morphology in the liver tissues. WPPH had the best effect, which was similar to that of silymarin.

Expression of nNOS, eNOS, and iNOS in the Liver of Mice
nNOS and eNOS mRNA and protein expressions were the highest in liver tissues of the normal group, while iNOS mRNA and protein expressions were the lowest in liver tissues of the normal group (Table 6 and Figure 4). Alcohol decreased the expression of nNOS and eNOS and increasede that of iNOS in the liver. WPP inhibited the enhanced iNOS expression and decreased nNOS and eNOS expressions. The effect of WPPH was similar to that of silymarin. Table 6. Neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS) mRNA expression in the hepatic tissues of the mice (N = 10).

Expression of nNOS, eNOS, and iNOS in the Liver of Mice
nNOS and eNOS mRNA and protein expressions were the highest in liver tissues of the normal group, while iNOS mRNA and protein expressions were the lowest in liver tissues of the normal group (Table 6 and Figure 4). Alcohol decreased the expression of nNOS and eNOS and increasede that of iNOS in the liver. WPP inhibited the enhanced iNOS expression and decreased nNOS and eNOS expressions. The effect of WPPH was similar to that of silymarin.  Figure 4. nNOS, eNOS, and iNOS protein expression in the liver tissues of mice. a-d Using Tukey's honestly significant different test, there was no significant difference between the two groups with the same superscript (p > 0.05), and there was a significant difference between the two groups with different superscript (p < 0.05). Silymarin: mouse treated with 100 mg/kg silymarin; WPPL: mouse treated with 50 mg/kg white peony polyphenols; WPPH: mouse treated with 100 mg/kg white peony polyphenols.

Expression of Cu-Zn-SOD, Mn-SOD, and CAT in the Liver of Mice
Mn-SOD, Cu-Zn-SOD, and CAT mRNA and protein expressions in the liver tissues were the highest in the model group, while iNOS expression was the lowest (Table 7 and Figure 5). Silymarin administered by gavage significantly upregulated Mn-SOD, Cu-Zn-SOD, and CAT expression levels in the livers of mice with liver injury. WPP also upregulated their expression, and the effect of WPPH was greater but did not differ significantly from that of silymarin (p > 0.05). Table 7. Cu-Zn superoxide dismutase (Cu-Zn-SOD), Mn-SOD, and catalase (CAT) mRNA expression in the hepatic tissues of the mice (N = 10).  Figure 4. nNOS, eNOS, and iNOS protein expression in the liver tissues of mice. a-d Using Tukey's honestly significant different test, there was no significant difference between the two groups with the same superscript (p > 0.05), and there was a significant difference between the two groups with different superscript (p < 0.05). Silymarin: mouse treated with 100 mg/kg silymarin; WPPL: mouse treated with 50 mg/kg white peony polyphenols; WPPH: mouse treated with 100 mg/kg white peony polyphenols.

Expression of Cu-Zn-SOD, Mn-SOD, and CAT in the Liver of Mice
Mn-SOD, Cu-Zn-SOD, and CAT mRNA and protein expressions in the liver tissues were the highest in the model group, while iNOS expression was the lowest (Table 7 and Figure 5). Silymarin administered by gavage significantly upregulated Mn-SOD, Cu-Zn-SOD, and CAT expression levels in the livers of mice with liver injury. WPP also upregulated their expression, and the effect of WPPH was greater but did not differ significantly from that of silymarin (p > 0.05). Table 7. Cu-Zn superoxide dismutase (Cu-Zn-SOD), Mn-SOD, and catalase (CAT) mRNA expression in the hepatic tissues of the mice (N = 10). "±" for standard deviation (N = 10/group). a-e Using Tukey's honestly significant different test, there was no significant difference between the two groups with the same superscript (p > 0.05), and there was a significant difference between the two groups with different superscript (p < 0.05). Silymarin: mouse treated with 100 mg/kg silymarin; WPPL: mouse treated with 50 mg/kg white peony polyphenols; WPPH: mouse treated with 100 mg/kg white peony polyphenols.

GAPDH Cu/Zn-SOD Mn-SOD CAT
Antioxidants 2019, 8, x FOR PEER REVIEW 10 of 15 Figure 5. Cu-Zn-SOD, Mn-SOD, and CAT protein expression in the liver tissues of mice. a-d Using Tukey's honestly significant different test, there was no significant difference between the two groups with the same superscript (p > 0.05), and there was a significant difference between the two groups with different superscript (p < 0.05). Silymarin: mouse treated with 100 mg/kg silymarin; WPPL: mouse treated with 50 mg/kg white peony polyphenols; WPPH: mouse treated with 100 mg/kg white peony polyphenols.

Analysis of the Chemical Composition of WPPs
The HPLC results showed that WPPs were rich in gallic acid, catechin, and hypericin with contents of 66.93 mg/g, 427.06 mg/g, and 168.50 mg/g, respectively ( Figure 6).

Figure 5.
Cu-Zn-SOD, Mn-SOD, and CAT protein expression in the liver tissues of mice. a-d Using Tukey's honestly significant different test, there was no significant difference between the two groups with the same superscript (p > 0.05), and there was a significant difference between the two groups with different superscript (p < 0.05). Silymarin: mouse treated with 100 mg/kg silymarin; WPPL: mouse treated with 50 mg/kg white peony polyphenols; WPPH: mouse treated with 100 mg/kg white peony polyphenols.

Analysis of the Chemical Composition of WPPs
The HPLC results showed that WPPs were rich in gallic acid, catechin, and hypericin with contents of 66.93 mg/g, 427.06 mg/g, and 168.50 mg/g, respectively ( Figure 6).

Discussion
DPPH is a stable radical. ABTS has intermediate stability, and laccase activity can be determined by the rate of laccase oxidation of ABTS [14]. Detecting the scavenging capability of a substance toward DPPH and ABTS can preliminarily determine its antioxidation activity [15]. WPP has good scavenging capability toward DPPH and ABTS, and thus has good antioxidative activity in vitro.
The liver plays an important role in maintaining homeostasis and regulating body functions, including growth and development, disease resistance, and energy supply. With changes in lifestyle and dietary structure as well as increased alcohol production, the proportion of people drinking alcohol is increasing. The incidence of alcohol-related diseases is also increasing yearly. Chronic alcoholism can lead to alcoholic hepatitis, fatty liver, liver cirrhosis, and other alcohol-related diseases [16]. As a classic liver-protecting drug, silymarin has been applied to treat various types of liver injury for a long time. Silymarin has many effects, such as antioxidation, anti-inflammation, immune regulation, and cell regeneration [17]. In this study, it was used as a positive control drug. Liver weight and liver indices were determined to evaluate liver injury induced by carbon tetrachloride. High liver weight and liver indices are manifestations of liver injury [18]. Our study confirmed this and indicated that WPPs downregulated the liver weight and indices in mice with liver injury, and the effects were similar to those of silymarin.
AST is mainly distributed in hepatocytic plasma and mitochondria, and ALT is mainly found in hepatocytic plasma. Hepatocytic necrosis leads to increased ALT and AST levels in the body [19]. Lipid peroxidation in the liver alters hepatocyte membrane permeability, and ALP levels increase sharply [20]. Liver injury may lead to intrahepatic diffusion of fatty acids, resulting in elevated TG

Discussion
DPPH is a stable radical. ABTS has intermediate stability, and laccase activity can be determined by the rate of laccase oxidation of ABTS [14]. Detecting the scavenging capability of a substance toward DPPH and ABTS can preliminarily determine its antioxidation activity [15]. WPP has good scavenging capability toward DPPH and ABTS, and thus has good antioxidative activity in vitro.
The liver plays an important role in maintaining homeostasis and regulating body functions, including growth and development, disease resistance, and energy supply. With changes in lifestyle and dietary structure as well as increased alcohol production, the proportion of people drinking alcohol is increasing. The incidence of alcohol-related diseases is also increasing yearly. Chronic alcoholism can lead to alcoholic hepatitis, fatty liver, liver cirrhosis, and other alcohol-related diseases [16]. As a classic liver-protecting drug, silymarin has been applied to treat various types of liver injury for a long time. Silymarin has many effects, such as antioxidation, anti-inflammation, immune regulation, and cell regeneration [17]. In this study, it was used as a positive control drug. Liver weight and liver indices were determined to evaluate liver injury induced by carbon tetrachloride. High liver weight and liver indices are manifestations of liver injury [18]. Our study confirmed this and indicated that WPPs downregulated the liver weight and indices in mice with liver injury, and the effects were similar to those of silymarin.
AST is mainly distributed in hepatocytic plasma and mitochondria, and ALT is mainly found in hepatocytic plasma. Hepatocytic necrosis leads to increased ALT and AST levels in the body [19]. Lipid peroxidation in the liver alters hepatocyte membrane permeability, and ALP levels increase sharply [20]. Liver injury may lead to intrahepatic diffusion of fatty acids, resulting in elevated TG content in the liver. TG and TC also reflect lipid peroxidation in the liver, which elevates the TG and TC levels in the body [21]. Decreased liver function can further damage the renal function and increase BUN (a protein metabolite in the blood). Liver injury also affects ALB synthesis, transportation, and release in the body, decreasing ALB levels in the blood [22]. In this study, WPPs also restored the above blood indices to normal values, achieving the same effect as those of silymarin.
Long-term drinking of alcohol leads to oxidation in the body, and the body defends itself against oxidative injury via nonenzymatic and enzymatic antioxidation, for example by regulating and increasing SOD, CAT, and GSH-Px activities [23]. SOD catalyzes the dismutation of superoxide free radicals and scavenges free radicals. CAT and SOD also have synergistic effects which strengthens their effect of scavenging free radicals [24]. CAT is an important antioxidase in the body and can scavenge H 2 O 2 from the body, thus inhibiting oxidative stress, reducing alcohol-induced oxidation in the body, and inhibiting liver injury [25]. GSH-Px catalyzes hydrogen peroxide decomposition. By catalyzing the reduction of hydrogen peroxide, it can protect cell membranes and avoid cell injury [18]. MDA is a metabolite of lipid peroxidation and is massively accumulated in the body after liver injury [26]. Increases in NO and other oxidative products content can damage phospholipids and proteins on the cell surface and further promote inflammatory exudation and injury [27]. NO reacts with superoxide anion, generating ONOO -, aggravates oxidative stress reactions, and leads to cytotoxicity, aggravating liver injury [28]. WPPs upregulated serum SOD, CAT, and GSH-Px levels and downregulated MDA and NO levels, playing a role in counteracting liver injury.
Alcohol can cause oxidation in the body and promote inflammation in the liver, which is reflected by significant increases in the serum levels of IL-6, IL-12, TNF-α, and IFN-γ in mice [29]. IL-6 is secreted by Th2 cells and participates in the humoral immune response. When Th2 levels increase in the body, visceral functions may be damaged [30]. IL-6 can promote differentiation and proliferation of T lymphocytes and antibody generation, alter intracellular G cell activity, upregulate neutrophil function, and enhance inflammatory responses in the body [31]. IL-12 is a strong effective activator of NK cells. Excessive hepatocyte apoptosis and an overly strong immune response aggravates liver injury, partly because of the increased killing function of CD8 + T cells by IL-12 [32]. TNF-α binding to the receptor TNF-α R1 on the hepatocyte membrane can lead to the transformation of intracellular double-stranded genomic DNA into oligodeoxynucleotide fragments and promote stem cell apoptosis. TNF-α also aggravates inflammation and liver injury by activating NF-κB [33]. IFN-γ is a proinflammatory cytokine and can enhance the sensitivity of liver cells to TNF-α, rendering liver cells susceptible to damage [34]. Oxidative stress appearing after liver tissue injury can cause an imbalance in inflammatory factors, including IL-6, IL-12, TNF-α, and IFN-γ, in the body, thus increasing their contents in the liver [35]. WPPs can alleviate liver injury by regulating the serum levels of IL-6, IL-12, TNF-α, and IFN-γ in mice with liver injury, and the effect is similar to that of silymarin. nNOS protects nerve cells and tissues and helps repair damaged tissues [36]. eNOS expression in tissues is relatively stable, and NO generated by eNOS promotes the repair of the liver tissue. eNOS also promotes revascularization further enhancing the repair of damaged liver tissue [37]. After iNOS is activated, the enzyme activity can last a long time, thus releasing a large amount of NO. Low-concentration NO can inhibit gene mutations and enhance the body's defenses. However, excessive NO stimulates gene mutation and further causes tissue lesions [38]. Oxidative stress leads to aggravated inflammation, resulting in iNOS overexpression, nNOS and eNOS underexpression, and liver injury aggravation [39]. WPPs can upregulate nNOS and eNOS expression, downregulate iNOS expression, and further control the aggravation of inflammation and liver injury caused by oxidative stress, thus exerting a protective effect on the liver.
Mn-SOD and Cu-Zn-SOD are isomers of SOD in the body. Mn-SOD is a SOD free-radical scavenger with Mn 4+ as its activity center in the mitochondria. Cu-Zn-SOD is another SOD free-radical scavenger that exists in the cytoplasm, having Cu 2+ and Zn 2+ as its activity centers [40]. The liver and heart are the organs with the highest number of mitochondria. After alcohol-induced liver injury, Mn-SOD activity significantly declines, which is consistent with the results of our study [34]. Cu-Zn-SOD can eliminate the toxic effect caused by O 2− •, thus protecting the gastric tissue [41]. Alcohol causes oxidative stress in the body and generates many free radicals. Mn-SOD and Cu-Zn-SOD can inhibit free radicals in the body, helping to prevent liver injury [42]. WPPs can increase the mRNA and protein expressions of Mn-SOD and Cu-Zn-SOD in the liver and further repair liver injury.
As a polyphenol, gallic acid (GA) has strong antioxidation and anti-free radical effects. It can reduce DNA damage in cells caused by oxidation and free radicals and protect tissues [43]. Catechin has a regulatory and antioxidative function in cells, and its antioxidation activity is higher than that of vitamin E, which can efficiently scavenge the free radicals generated in the body and protect cell membranes [44]. Hyperoside is also a strong antioxidant and protects the liver. Its mechanism of action is related to its antioxidative properties, to the normalization of NO levels, and to the improvement of SOD activity [45]. Three substances in WPPs contribute to their protective effects against alcoholic liver injury. Hypericin is uncommon in tea, but present at high levels in white peony, and has biological activity.

Conclusions
In our study, WPPs reduced the liver indices of mice with liver injury and regulated the related serum levels and the inflammation-related cytokine levels, returning them to normal values. qPCR and western blot further demonstrated that WPPs effectively restored the mRNA and protein expression levels of various liver injury markers in the livers of mice with liver injury. Thus, WPPs have a good attenuating effect on alcoholic liver injury, and their efficacy is similar to that of silymarin. WPPs are a promising natural resource. The possible synergistic effect between the key polyphenols in WPPs should be further investigated.