Tempol Supplementation Restores Diaphragm Force and Metabolic Enzyme Activities in mdx Mice

Duchenne muscular dystrophy (DMD) is characterized by striated muscle weakness, cardiomyopathy, and respiratory failure. Since oxidative stress is recognized as a secondary pathology in DMD, the efficacy of antioxidant intervention, using the superoxide scavenger tempol, was examined on functional and biochemical status of dystrophin-deficient diaphragm muscle. Diaphragm muscle function was assessed, ex vivo, in adult male wild-type and dystrophin-deficient mdx mice, with and without a 14-day antioxidant intervention. The enzymatic activities of muscle citrate synthase, phosphofructokinase, and lactate dehydrogenase were assessed using spectrophotometric assays. Dystrophic diaphragm displayed mechanical dysfunction and altered biochemical status. Chronic tempol supplementation in the drinking water increased diaphragm functional capacity and citrate synthase and lactate dehydrogenase enzymatic activities, restoring all values to wild-type levels. Chronic supplementation with tempol recovers force-generating capacity and metabolic enzyme activity in mdx diaphragm. These findings may have relevance in the search for therapeutic strategies in neuromuscular disease.


Introduction
Duchenne muscular dystrophy (DMD) is the most common form of inherited muscle disease in childhood, with an estimated incidence of 1:3500 male births [1]. DMD is caused by a deficiency in the protein dystrophin, which is a component of the dystrophin associated protein complex (DAPC) [2]. The DAPC has a structural role in linking the actin cytoskeleton to the extracellular matrix, thus stabilizing the sarcolemma during muscle contraction and relaxation [3]. Dystrophin deficiency results in destabilization of the DAPC, leading to muscle weakness and fragility, resulting in muscle damage, fibrosis, and necrosis [4]. Inflammation is secondary to muscle damage in DMD, with attendant disruption to Ca 2+ homeostasis, oxidative stress, and mitochondrial dysfunction [5]. DMD patients suffer severe limb and respiratory muscle weakness [6]. Patients have compromised lung function due to diaphragm muscle weakness, altered chest wall compliance, and scoliosis [7]. Trans-diaphragmatic pressures are low in DMD boys [8], and disordered breathing can occur, particularly during sleep, leading to obstructive sleep apnea and hypoventilation [9]. Cardio-respiratory failure is the leading cause of death in DMD.
Oxidative stress is the result of increased reactive oxygen species (ROS) and/or decreased antioxidant capacity. ROS, in particular superoxide anions, are highly chemically reactive substances that can react with nucleic acids, lipids, and proteins, thus hindering cellular metabolism, resulting in cell injury [10]. Low levels of ROS are essential for optimal force production during muscle contraction, and act as important physiological signaling molecules that alter enzymatic activity and n = 7) and mdx + tempol in vivo (30.1 ± 2.2g; n = 9); mdx animals were randomly assigned to groups. The mdx + tempol in vivo group received tempol (1 mM 4-hydroxy-TEMPO; Sigma Aldrich, Wicklow, Ireland) in their drinking water for two weeks (from 12 to 14 weeks of age), equivalent to a dose of 20-35 mg/g body weight, taking an estimate of fluid intake and known body mass gain over the intervention period. The dose was informed by previously published work in rat, wherein tempol proved efficacious in preventing respiratory muscle dysfunction in response to redox stress associated with exposure to chronic intermittent hypoxia [29,30].

Ex Vivo Muscle Preparation
Animals were anaesthetized with 5% isoflurane in air, and euthanized by cervical dislocation. Diaphragm muscle was immediately excised, with central tendon and rib intact for functional studies. Additional diaphragm muscle samples were snap frozen in liquid nitrogen and stored at −80 • C for later analysis. Diaphragm muscle was suspended vertically in a water-jacketed tissue bath at 35 • C, filled with Krebs solution (in mM: 120 NaCl, 5 KCl, 2.5 Ca 2+ gluconate, 1.2 MgSO 4 , 1.2 NaH 2 PO 4 , 25 NaHCO 3 and 11.5 glucose) and D-tubocurarine (25 µM). Preparations were equilibrated with hyperoxic (95% O 2 /5% CO 2 ) gas. The rib end of the preparation was attached to an immobile hook at the base of a muscle holder, and the central tendon was attached to a dual mode lever transducer system (Aurora Scientific Inc., Aurora, ON, Canada) with non-elastic string, to allow the assessment of isometric and isotonic contractile properties. For the mdx + tempol in vitro group, diaphragm muscle from mdx mice was studied in Krebs solution containing 1 mM tempol, which has been shown to exert positive inotropic effects on rat respiratory muscle in isolated muscle preparations [28,29]).

Isometric Protocol
The muscle strips were stimulated via field stimulation with platinum electrodes at supramaximal voltage. Optimal length (L o ), the length producing maximum twitch force, was obtained by repeated isometric twitch stimulation (supramaximal voltage; 1 ms duration) at varying muscle lengths, achieved by adjustment of a micro-positioner. The L o was recorded, and the muscle remained at this length for the remainder of the experiment. Following a 10 min equilibration period, contractile properties were examined. First, a single twitch was elicited, and twitch force, contraction time (CT; time to peak force), and half-relaxation time ( 1 2 RT; time for force to decay by 50%) were determined. Next, an isometric tetanic contraction was elicited by stimulating muscle strips with supramaximal voltage at 100 Hz for 300 ms duration. Peak isometric tetanic force (F max ) was determined [34,35].

Tissue Preparation
Diaphragm samples stored at −80 • C were removed and allowed to defrost at 4 • C for 5 min. All procedures were performed at 4 • C to prevent protein degradation. Samples were homogenized in a lysis buffer (RIPA) made up from 10× RIPA, deionized water, 200 mM sodium fluoride, 100 mM phenylmethylsulfonyl fluoride, protease cocktail inhibitor 1, and phosphatase cocktail inhibitor 2.
Following the homogenization process, the reactant mixtures were centrifuged (15,339× g) and the supernatants were harvested. Total amount of protein for each tissue sample was determined using Pierce ® Bicinchoninic Acid Assay (BCA assay, Thermo Scientific, Fisher, Dublin, Ireland). Supernatants were aliquoted and stored at −80 • C for future use.

Metabolic Enzyme Assays
Tissue homogenates were used for enzymatic activity assays. The experimental procedures for citrate synthase, phosphofructokinase, and lactate dehydrogenase activity assays were performed in accordance with the technical bulletins of a citrate synthase assay kit (CS0720; Sigma-Aldrich, Wicklow, Ireland), phosphofructokinase activity colorimetric assay kit (MAK093; Sigma-Aldrich), and lactate dehydrogenase activity assay kit (MAK066; Sigma-Aldrich), respectively. Results are presented as enzymatic activity per 1 mg of protein in tissue homogenate.

Data Analysis
Specific force was normalized for muscle tissue cross-sectional area (CSA), and calculated in N/cm 2 . Muscle CSA was estimated for each muscle strip by dividing the muscle mass (weight in grams) by the product of muscle L o (cm) and muscle density (assumed to be 1.06 g/cm 3 ). For isotonic load relationships, data were expressed as the measured parameter versus % load. Total muscle shortening was normalized to L o and expressed in L o /s. Maximal total shortening (S max ) and maximum shortening velocity (V max ) occurred at 0% load. Maximum mechanical work (W max ) and power (P max ) occurred at~30-40% load.

Statistical Analysis
Values are expressed as mean ± SD or are represented graphically as box and whisker plots (median, 25th-75th centile, and minimum and maximum). Data were statistically compared by unpaired Student's t tests with Welch's correction where appropriate, and two-way analysis of variance (ANOVA); for data from incremental load tests). p < 0.05 was deemed to be statistically significant.

Isometric Force and Twitch Contractile Kinetics
Representative original traces for diaphragm muscle twitch (A) and tetanic (B) contractions and maximum unloaded shortening (C) are shown in Figure 1 for wild-type, mdx, mdx + tempol in vitro and mdx + tempol in vivo. Table 1 shows data for diaphragm muscle twitch force and contractile kinetics from all four groups. Twitch force was significantly lower in mdx compared with wild-type diaphragm (p = 0.0066, Student's t test). There was a significant decrease in diaphragm 1 2 RT following tempol administration in vivo in mdx mice (p = 0.0046). Twitch force was significantly higher in mdx + tempol in vivo compared with mdx (p = 0.0111). F max was significantly lower in mdx diaphragm compared with wild-type (p < 0.0001; Figure 1D). F max was significantly higher in mdx + tempol, in vivo, compared with mdx (p = 0.0069), such that values were equivalent to wild-type values for peak force generation. Bath application of tempol to mdx diaphragm had no effect on force-generating capacity or contractile kinetics compared with mdx ( Figure 1 and Table 1).

Figure 1.
Peak Isometric Tetanic Force. Representative traces for muscle twitch (A) and tetanic (B) contractions and maximum unloaded shortening (C) for diaphragm muscle from wild-type, mdx, mdx + tempol in vitro and mdx + tempol in vivo. Group data for diaphragm muscle peak tetanic force (D) from wild-type (n = 7), mdx (n = 7), mdx + tempol in vitro (n = 7) and mdx + tempol in vivo (n = 8). For the mdx + tempol in vitro group, diaphragm muscle preparations were studied in Krebs solution containing 1 mM tempol in vitro. The mdx + tempol in vivo group received 1 mM tempol in their drinking water for two weeks. Values are expressed as box and whisker plots (median, 25-75% centiles and minimum and maximum values) and data were statistically compared by Student's t tests. *** p < 0.0001; ** p = 0.0069. Values (mean ± SD) for twitch contraction time (CT), twitch half-relaxation time (½ RT), peak twitch force (Pt), maximum mechanical work (Wmax), maximum mechanical power (Pmax), peak shortening (Smax), and peak shortening velocity (Vmax) of diaphragm muscle from the following groups: wild-type (n = 7), mdx (n = 7), mdx + contractions and maximum unloaded shortening (C) for diaphragm muscle from wild-type, mdx, mdx + tempol in vitro and mdx + tempol in vivo. Group data for diaphragm muscle peak tetanic force (D) from wild-type (n = 7), mdx (n = 7), mdx + tempol in vitro (n = 7) and mdx + tempol in vivo (n = 8). For the mdx + tempol in vitro group, diaphragm muscle preparations were studied in Krebs solution containing 1 mM tempol in vitro. The mdx + tempol in vivo group received 1 mM tempol in their drinking water for two weeks. Values are expressed as box and whisker plots (median, 25-75% centiles and minimum and maximum values) and data were statistically compared by Student's t tests. *** p < 0.0001; ** p = 0.0069.  Values (mean ± SD) for twitch contraction time (CT), twitch half-relaxation time ( 1 2 RT), peak twitch force (P t ), maximum mechanical work (W max ), maximum mechanical power (P max ), peak shortening (S max ), and peak shortening velocity (V max ) of diaphragm muscle from the following groups: wild-type (n = 7), mdx (n = 7), mdx + tempol in vitro (n = 7) and mdx + tempol in vivo (n = 8). For the mdx + tempol in vitro group, diaphragm muscle preparations were studied in Krebs solution containing 1 mM tempol in vitro. The mdx + tempol in vivo group received 1 mM tempol in their drinking water for two weeks. Data were statistically compared by unpaired Student's t tests with Welch's correction where appropriate. $: Wild-Type vs. mdx; †: mdx vs. mdx Tempol in vitro; £: mdx vs. mdx Tempol in vivo. Table 1 shows data for diaphragm muscle isotonic contractile parameters. W max was significantly lower in mdx compared with wild type diaphragm (p = 0.0276; Student t test). W max was significantly elevated in mdx + tempol in vivo compared with mdx (p = 0.0085). P max was lower in mdx diaphragm compared with wild-type, but this did not reach statistical significance (p = 0.0709). P max was significantly elevated in mdx + tempol in vivo compared with mdx (p = 0.0217), completely restoring P max to wild-type values. No significant differences were noted for S max between groups. Bath application of tempol to mdx diaphragm significantly decreased V max compared with mdx (p = 0.0353), but had no effect on W max or P max . Figure 2A-D shows data for diaphragm muscle isotonic load relationships. Loading had a significant effect on work (p < 0.0001; two-way ANOVA; Figure 2A), power (p < 0.0001; Figure 2B), shortening (p < 0.0001; Figure 2C) and shortening velocity (p < 0.0001; Figure 2D) for diaphragm muscle from all four groups. Work (p = 0.0071) and power production (p = 0.0115) were significantly reduced in mdx diaphragm compared with wild-type. Tempol supplementation in vivo in mdx mice significantly increased work (p = 0.0063) and power (p = 0.0177) production compared with mdx. Bath application of tempol significantly reduced mdx diaphragm power production (p = 0.0037) and shortening velocity (p = 0.0159) compared with mdx. Table 1 shows data for diaphragm muscle isotonic contractile parameters. Wmax was significantly lower in mdx compared with wild type diaphragm (p = 0.0276; Student t test). Wmax was significantly elevated in mdx + tempol in vivo compared with mdx (p = 0.0085). Pmax was lower in mdx diaphragm compared with wild-type, but this did not reach statistical significance (p = 0.0709). Pmax was significantly elevated in mdx + tempol in vivo compared with mdx (p = 0.0217), completely restoring Pmax to wild-type values. No significant differences were noted for Smax between groups. Bath application of tempol to mdx diaphragm significantly decreased Vmax compared with mdx (p = 0.0353), but had no effect on Wmax or Pmax. Figure 2A-D shows data for diaphragm muscle isotonic load relationships. Loading had a significant effect on work (p < 0.0001; two-way ANOVA; Figure 2A), power (p < 0.0001; Figure 2B), shortening (p < 0.0001; Figure 2C) and shortening velocity (p < 0.0001; Figure 2D) for diaphragm muscle from all four groups. Work (p = 0.0071) and power production (p = 0.0115) were significantly reduced in mdx diaphragm compared with wild-type. Tempol supplementation in vivo in mdx mice significantly increased work (p = 0.0063) and power (p = 0.0177) production compared with mdx. Bath application of tempol significantly reduced mdx diaphragm power production (p = 0.0037) and shortening velocity (p = 0.0159) compared with mdx.

Metabolic Enzyme Activity
Figure 3A-C shows data for diaphragm muscle metabolic enzyme activities. Citrate synthase activity was significantly lower in mdx diaphragm compared with wild-type (p = 0.0003; unpaired Student's t test; Figure 3A). Chronic tempol supplementation in mdx significantly increased diaphragm Antioxidants 2017, 6, 101 7 of 16 citrate synthase activity compared with mdx (p = 0.005). No significant differences were noted between groups for diaphragm phosphofructokinase activity ( Figure 3B).
Lactate dehydrogenase activity was significantly lower in mdx diaphragm compared with wild-type (p = 0.001; Figure 3C). Chronic tempol supplementation in mdx significantly increased diaphragm lactate dehydrogenase activity compared with mdx (p = 0.0018).

Metabolic Enzyme Activity
Figure 3A-C shows data for diaphragm muscle metabolic enzyme activities. Citrate synthase activity was significantly lower in mdx diaphragm compared with wild-type (p = 0.0003; unpaired Student's t test; Figure 3A). Chronic tempol supplementation in mdx significantly increased diaphragm citrate synthase activity compared with mdx (p = 0.005). No significant differences were noted between groups for diaphragm phosphofructokinase activity ( Figure 3B).
Lactate dehydrogenase activity was significantly lower in mdx diaphragm compared with wildtype (p = 0.001; Figure 3C). Chronic tempol supplementation in mdx significantly increased diaphragm lactate dehydrogenase activity compared with mdx (p = 0.0018).

Discussion
The main findings of this study are (1) diaphragm muscle weakness in mdx mice is evidenced by reduced specific force, work and power output; (2) dystrophin-deficiency in mdx diaphragm is associated with reduced citrate synthase and lactate dehydrogenase enzyme activities, whereas phosphofructokinase activity is equivalent to wild-type; (3) chronic tempol supplementation in mdx mice completely restored diaphragm force-and power-generating capacity; (4) chronic tempol supplementation significantly increased mdx diaphragm citrate synthase and lactate dehydrogenase enzyme activities to wild-type levels; (5) acute bath application of tempol had limited effects on dystrophic diaphragm function ex vivo.
Diaphragm muscle thickening and fat accumulation are described in DMD patients, associated with diaphragm muscle weakness [38,39]. Ventilatory capacity decreases with age, since DMD is a progressive disease [8,40]. Respiratory failure is a leading cause of mortality in DMD. Although DMD is primarily caused by a genetic abnormality resulting in the absence of the dystrophin protein, many secondary pathologies have been identified as contributing to the muscle pathology observed in DMD, including inflammation, Ca 2+ dysregulation, and redox stress [5,26].
Diaphragm muscle degeneration, fibrosis, and dysfunction are reported in mdx mice, which display an overt pathology similar to human DMD [16]. In the present study, we report diaphragm weakness in mdx mice consistent with other reports [15,16,41]. Diaphragm weakness in mdx was characterized by reduced twitch and tetanic muscle force and reduced W max . Diaphragm muscle mechanical work and power generation were reduced across the load continuum, revealing severe intrinsic mechanical dysfunction in dystrophic diaphragm muscle. These functional deficits were associated with altered metabolic enzyme activities, characterized by reduced citrate synthase and lactate dehydrogenase activity. Citrate synthase catalyzes the first step within the Krebs cycle, the condensation of acetyl-coenzyme A with oxaloacetate to form citrate, and serves as a marker of the mitochondrial matrix. Reduced citrate synthase activity suggests reduced mitochondrial activity, and hence, aerobic capacity in mdx diaphragm. This reduction in mitochondrial activity may relate to a loss of mitochondrial content, resulting in reduced oxidative capacity, or may be due to oxidative stress-induced mitochondrial dysfunction or mitophagy. Phosphofructokinase catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, thus functioning as a key regulatory step in the glycolytic pathway. Phosphofructokinase activity was unchanged in mdx diaphragm compared with wild-type. Lactate dehydrogenase catalyzes the forward and backward conversion of pyruvate to lactate, with the skeletal muscle isoform kinetically favoring conversion from pyruvate to lactate. Since glycolysis, which diverts pyruvate to lactate, serves to diminish redox stress [42], reductions in lactate dehydrogenase activity can cause oxidative stress associated with increased cellular oxygen consumption [43]. Metabolic reprogramming in mdx and DMD muscle is complex, and likely temporally regulated in dynamic fashion. Indeed, contrary to our observation, a recent report revealed elevated activity in oxidative enzymes of the Krebs cycle in mdx diaphragm [44].
Indicators of oxidative stress, such as 4-hydroxynonenal (4-HNE) and dihydroethdium (DHE), are increased in mdx diaphragm, revealing increased oxidative stress which likely has functional implications [17]. Antioxidant enzymes, such as superoxide dismutase, catalase and glutathione peroxidase, are elevated in limb muscle from mdx mice [20], which may contribute to the mild limb muscle phenotype which is observed in mdx mice compared with the diaphragm muscle, which more closely represents the human diaphragm pathology. Studies have shown that NOX subunits and superoxide production are increased in skeletal muscle of mdx mice [21,45]. Oxidative stress contributes to cardiomyocyte dysfunction in dystrophic hearts, and NOX2 inhibition resulted in restoration of calcium handling and contractility, and reduced collagen expression in cardiomyocytes [19,24]. The NOX subunits gp91 phox , p67 phox and Rac1 are increased in the tibialis anterior of mdx mice, associated with increased superoxide production [21].
Tempol has previously been shown to have positive inotropic effects on rat pharyngeal dilator (respiratory) muscle performance ex vivo [28,29]. Interestingly, tempol was shown to ameliorate upper airway muscle weakness in a rat model of chronic intermittent hypoxia [29]. In a similar model of chronic intermittent hypoxia, tempol exerted modest effects on diaphragm muscle force, but reversed chronic intermittent hypoxia-induced diaphragm fatigue [30]. Chronic tempol supplementation has also been shown to prevent sustained hypoxia induced pharyngeal dilator muscle weakness [31], but not diaphragm muscle dysfunction [37]. Collectively, these studies illustrate that tempol can exert inotropic effects on rodent respiratory muscle and ameliorate muscle weakness in models of hypoxic stress via superoxide scavenging, although this capacity appears muscle specific and may be dependent on the mode of hypoxia (sustained versus intermittent). This may have relevance to putative hypoxia-dependent remodeling in mdx muscle. DMD patients [9,46] and mdx mice [47,48] hypoventilate. DMD patients exhibit nocturnal hypoxemia and sleep-disordered breathing [49,50], and exposure to chronic intermittent hypoxia has been shown to further weaken mdx diaphragm [51]. Therefore, hypoxic stress may be implicated in mdx and DMD pathology, such that antioxidant strategies (such as tempol), proven to prevent hypoxia-dependent respiratory muscle weakness and fatigue, may be especially useful.
Since elevated superoxide production is reported in the diaphragm muscle of mdx mice, we sought to examine the efficacy of the superoxide scavenger tempol on mdx diaphragm muscle function. To our knowledge, this is the first report on the effects of tempol on muscle function and metabolic enzyme activity in the mdx mouse. Chronic tempol supplementation resulted in increased diaphragm force generation, revealed both in twitch and tetanic contractions, impressively restoring these functional indices to values equivalent to wild-type values. Work-and power-generating capacity expressed as a function of load were significantly increased in diaphragm from tempol supplemented mice compared with mdx, resulting in a complete restoration of work-and power-generating capacity. W max and P max were similarly significantly increased. Interestingly, tempol decreased 1 2 RT for mdx diaphragm, suggesting an increased rate of calcium reuptake into the sarcoplasmic reticulum during muscle relaxation. These functional improvements in mdx diaphragm are likely attributable to scavenging of excessive ROS in mdx diaphragm [17,21,45], and improvements in calcium handling.
In the current study, the finding that acute bath application of tempol significantly reduced mdx diaphragm V max , demonstrates the functional significance of ROS signaling, most likely superoxide, within mdx diaphragm muscle preparations. Basal ROS are known to have physiological roles in cross-bridge cycling and muscle force generation [11]. However, elevated levels of ROS orchestrate a pro-oxidant environment, which can serve to impair cross-bridge cycling, and thus, reduce force production. A tonic inhibitory effect of ROS on respiratory muscle function, suppressing force-generating capacity, was revealed by the observation of positive inotropic effects of acute bath application of the superoxide scavengers, tempol, and tiron [28]. Moreover, H 2 O 2 has been shown to cause concentration-dependent inotropic effects on rat respiratory muscle function, with high concentrations of ROS resulting in muscle weakness and fatigue, an effect blocked by catalase [52]. Notwithstanding the capacity for antioxidant scavenging to acutely affect muscle performance, the general lack of effect of bath application of tempol in mdx diaphragm is not surprising. We posit that chronically elevated levels of ROS in mdx diaphragm induce structural abnormalities that cannot be reversed by acute tempol application. Rather, it is evident that chronic antioxidant supplementation is required to re-establish redox homeostasis, returning ROS to physiological levels, limiting redox stress, allowing restoration of muscle function. The functional improvements in mdx diaphragm extended to restoration of citrate synthase and lactate dehydrogenase enzyme activities. Improved metabolic enzyme activity has obvious consequences for improved mdx diaphragm performance. We used enzyme activity as a surrogate marker for oxidative stress, since these proteins are often targets of redox stress, and can in turn be drivers of redox stress owing to alterations in bioenergetics. We interpret the restoration of aerobic and glycolytic enzyme activities as indirect evidence of the efficacy of tempol in ameliorating oxidative stress in mdx diaphragm, with the important added benefit of restoring energy homeostasis and ATP-generating capacity.
Previously, EUK-134, a novel catalytic mimetic of superoxide dismutase and catalase, was used to examine the role of oxidative stress in mdx diaphragm pathology. EUK-134 reduced markers of oxidative stress, inflammation, and indicators of muscle damage in mdx diaphragm, although only a partial rescue of diaphragm force was observed [53]. Treatment of mdx mice with ascorbic acid, an antioxidant and free radical scavenger, was shown to reduce creatine kinase levels, myonecrosis, inflammation, and the levels of 4-HNE [54]. These studies further highlight the close relationship between ROS and inflammation in dystrophic diaphragm. Beyond antioxidant effects, targeting ROS using antioxidant interventions in mdx mice may also have non-specific anti-inflammatory actions, further supporting their use as a potential therapeutic strategy in DMD. In respect of interventional studies in DMD, our data suggest that superoxide scavengers warrant attention. Tempol is not approved for use in humans, but dietary and/or pharmacotherapies mimicking its actions could prove beneficial in DMD. Many studies have revealed beneficial effects of antioxidants on muscle integrity ( Table 2), but few studies have assessed the effects of antioxidants on diaphragm force generating capacity. It will be interesting to determine how tempol compares to previous studies in respect of improvements in diaphragm structure and quality.
The current study has relevance to therapeutic interventions in DMD, particularly those aimed at alleviating respiratory deficits in DMD boys. Since diaphragmatic weakness is observed in DMD boys, and likely contributes to breathing disturbances and the development of respiratory failure, strategies aimed at improving diaphragm muscle functional capacity in DMD are attractive. In respect of the current data set, it would be interesting in future studies to examine the effects of tempol supplementation on respiratory and cardiac function in mdx mice.

Limitations
We acknowledge the current study employed a single dose of tempol at one time point in a progressive disease. However, notably, our intervention resulted in full recovery of diaphragm force. Future research should examine dose-dependent effects of tempol in mdx mice on respiratory and non-respiratory muscle structure and function. Mitochondrial function and direct measures of oxidative stress should be examined to determine the mechanism of force recovery in mdx diaphragm following tempol supplementation. EGCG had no effect on body weight and no observable toxic effects in the liver and kidney. EGCG decreased plasma CK and decreased the number of lipofuscin granules, necrotic muscle fibres and connective tissue in mdx diaphragm and increased utrophin expression. EGCG did not affect diaphragm isometric force. Amino acid mdx mouse 6 months old L-arginine in drinking water for 6 months Heart L-arginine had no effect on myocardial fibrosis but reduced the density of inflammatory cells in the mdx heart.

Conclusions
In conclusion, our findings show that tempol supplementation in mdx mice is efficacious, serving to restore diaphragm force-and power-generating capacity to levels equivalent to wild-type values. Functional improvements were accompanied by restoration of citrate synthase and lactate dehydrogenase enzyme activities in mdx diaphragm. Our study implicates superoxide anions and downstream ROS as pivotal mediators of dystrophin-deficient respiratory muscle pathophysiology. Recovery of diaphragm muscle contractile function was impressive in our study highlighting the potential utility of tempol and other superoxide scavengers in the treatment of DMD.