The Effects of Dietary n-3 Highly Unsaturated Fatty Acids on Growth, Antioxidant Capacity, Immunity, and Oxylipin Profiles in Acipenser dabryanus

Currently, the effects of dietary levels of n-3 highly unsaturated fatty acids (HUFAs) on the growth performance, antioxidant capacity, immunity, and serum oxylipin profiles of female F2-generation Yangtze sturgeon remain unknown. A total of 75 Yangtze sturgeons, an endangered freshwater fish species, with an average body weight of 3.60 ± 0.83 kg, were randomly allocated to 15 concrete pools, with each dietary group represented by 5 fish per pool. The fish were fed five different experimental diets containing various levels of n-3 HUFAs (0.5%, 1.0%, 1.5%, 2.0%, and 2.4%). After a feeding period of 5 months, no significant differences in the growth performances of the fish were observed among the five dietary groups (p > 0.05). However, we did note that the serum levels of low-density lipoprotein cholesterol (LDL-C), triglycerides (TGs), and total cholesterol (TCHO) exhibited a marked increase in the fish that consumed higher dietary n-3 HUFA levels (p < 0.05). Conversely, alkaline phosphatase (ALP) activities showed a notable decrease as dietary n-3 HUFA levels increased (p < 0.05). Serum antioxidant indices, such as the activity levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), were significantly higher in the 2.4% HUFA group compared to the 0.5% HUFA group. Additionally, muscle antioxidant indices, including total antioxidant capacity (T-AOC), catalase (CAT), and SOD activity, exhibited notable increases as dietary n-3 HUFA levels increased (p < 0.05). Furthermore, there was a decrease in malondialdehyde (MDA) levels as dietary n-3 HUFA levels increased (p < 0.05). In relation to immune indices, only serum immunoglobulin M (IgM) and muscle complement 3 (C3) were found to be influenced by dietary n-3 HUFA levels (p < 0.05). A total of 80 oxylipins were quantified, and our subsequent K-means cluster analysis resulted in the classification of 62 oxylipins into 10 subclasses. Among the different n-3 HUFA diets, a total of 14 differential oxylipins were identified in the sera. These findings demonstrate that dietary supplementation with n-3 HUFAs exceeding a 1.0% level can enhance antioxidant capacity and regulate serum lipid metabolism, potentially through modulation of oxylipins derived from ARA, DHA, and EPA. These insights provide novel perspectives on the mechanisms underlying these observations.


Introduction
Omega-6 arachidonic acid (ARA, C20:4n6), omega-3 eicosapentaenoic acid (EPA, 20:5n-3), and docosahexaenoic acid (DHA, 22:6n-3) are essential long-chain highly unsaturated fatty acids (LC-HUFAs) that play pivotal roles in the regulation of growth performance, contain approximately 42.54% crude protein and 10.68% crude lipid.Fish meal, soybean meal, chicken meal, and squid meal were used as the main protein sources.Wheat flour was used as the carbohydrate source.The lipid base comprised soybean oil, arachidonic acid oil (ARA), and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) oils in different amounts, with a gradual increase in DHA and EPA being prioritized in the diet formulations; final proportions of 6.18% (0.50%), 11.45 (1.00%), 15.53% (1.50%), 22.17% (2.00%), and 26.9 (2.40%) of total fatty acids were used.The EPA and DHA oils were derived from fish oil.The EPA oil content was 78.44%, and the main fatty acid profile of the EPA oil contained eicosanoic acid at 19.91%.The DHA oil content was 98.23%.The DHA and EPA oils were purchased from Shaanxi Pioneer Biotech Co., Ltd., Shanxi, Hanzhong, China.The ARA oil was purchased from Fuxing Biotechnology Co., Ltd., Hubei, Hanchuan, China.The raw materials of fish meal, soybean meal, chicken meal, squid meal, and wheat flour were purchased from Jinjia feed Co., Ltd., Zhejiang, Hangzhou, China.The diets were pelleted using a medium-sized laboratory meat grinder (MM32, Hunan Hengji Machinery Co., Ltd., Hunan, Yiyang, China); the pellets were 8 mm in diameter and approximately 20.0 mm in length, and they were stored at −20 • C until being fed to the Yangtze sturgeon.

Fish and Feeding Trial
At the Taihu Experimental Station of the Yangtze River Fisheries Research Institute in Jingzhou, Hubei province, 75 female sturgeon were randomly selected and cultured in concrete pools (length: 83.5 m; width: 2.0 m; and depth: 2.0 m) with a continuous groundwater and lake water complex flow system and continuous aeration.Prior to the experiment, these sturgeon had stage II gonads and an average body weight of 3.60 ± 0.83 kg.The selection of stage II experimental fish was primarily based on age, and three fish were randomly selected from a batch of experimental fish for HE staining to ensure the accuracy of our assessment of ovary development.Ovaries were fixed in 4% paraformaldehyde solution overnight, and then transferred to 70% ethanol until ready for paraffin sectioning.Slices with a thickness of 5 µm were obtained using a standard paraffin-embedding method, then stained with hematoxylin-eosin (HE).Images of the sections were captured using a Nikon Eclipse E100 (Tokyo, Japan) microscope equipped with a Nikon DS-U3 (Tokyo, Japan) imaging system.Histological sections of the ovaries are shown in Supplementary Figure S1.Yangtze sturgeon were randomly distributed into 15 indoor concrete pools (diameter: 3 m; depth: 0.5 m; n = 5/tank).A pair of female-specific primers (F: TAAT-CAATTGTAAGTCGCCAAG; R: ATTTTATTACGGTGAGTATACGAA) from our laboratory were used for sex identification, following the detailed process described in an existing report [27].Bright bands represent females, while no bands represent males; an amplified band is exhibited in Supplementary Figure S2.Throughout the experiment, the flow rate of the water was approximately 10.2 L/min.The water temperature varied between 17.4 • C and 22.2 • C (mean: 20.10 • C).The dissolved oxygen level of the water varied between 5.71 mg/L and 9.09 mg/L (mean: 7.42 mg/L).The pH of the water varied between 7.98 and 8.12 (mean: 8.04).The NH 4 -N concentration of the water varied between 0.07 mg/L and 0.28 mg/L (mean: 0.15 mg/L).Experimental fish were hand-fed 8 mm pellets to satiation twice daily (8:00 and 20:00) for five months.The feeding rate of the Yangtze sturgeon was approximately 0.8% of their body weight and adjusted on the basis of the weather conditions and the status of fish feeding.

Sample Collection and Analysis
2.3.1.Growth Performance, Biochemical, and Immunity Index Analysis After the feeding trial was completed, we did not feed the fish for 24 h.All surviving Yangtze sturgeon were collected from each concrete pool, counted, measured, and weighed.Two sampled fish from each tank were anaesthetized with MS-222 (GREENHX Biological Technology Co., Ltd., Beijing, China).After their body length was measured, 2 mL of blood was collected using a 5 mL syringe.The blood was then allowed to clot overnight in a 4 • C refrigerator.Serum samples were collected via centrifugation (3000× g/min 4 • C, 10 min), and they were placed in a −80 • C freezer to proceed with the biochemical indices, antioxidant capacity, immunity, and oxylipin analyses.
Back muscle samples were obtained using a copper probe (diameter: 5 mm; length: 16 cm) with a long and notched tube (diameter: 4 mm; length: 20 mm) on the end, and samples were placed in the −80 • C freezer for antioxidant capacity and immunity analyses.
To reduce the large differences in biological replicates, the serum and muscle samples used in the analyses of their biochemical indices, antioxidant capacity, immunity, and oxylipin were mixed samples taken from two fish; therefore, each treatment had three biological replicates.
The dietary crude protein, crude lipid, and ash contents were detected using the methods of AOAC (1995) [28].The dietary moisture content was analyzed by oven drying at 105 • C. The detection of dietary fatty acids was carried out following the methods of Wu et al. [29].

Serum Oxylipin Analysis
With respect to the HPLC conditions, the sample, extracts were analyzed using an LC-ESI-MS/MS system (ultraperformance liquid chromatography, UPLC; ExionLC TM AD; MS, QTRAP ® 6500+ System, Boston, MA, USA).The analytical conditions were as follows: HPLC-column, Waters ACQUITY UPLC HSS T3 C18 (100 mm × 2.1 mm i.d, 1.8 µm); solvent system-water with 0.04% acetic acid (A) and acetonitrile with 0.04% acetic acid (B).The gradient was 0-2.0 min from 0.1% to 30% B; 2.0-4.0 min at 50% B; and 4.0-5.5 min at 99% B, which was maintained for 1.5 min and at 6.0-7.0 min.It then reduced to 0.1% B and was maintained for 3.0 min.The flow rate was 0.4 mL/min; the temperature was 40 • C; and the injection volume was 10 µL.Regarding the ESI-MS/MS conditions, the ESI sources' operating parameters were as follows: ion source, ESI; source temperature, 550 • C; and ion spray voltage (IS), −4500 V.The curtain gas (CUR) was set at 35 psi.

Statistical Analysis
The data in the present study are shown as the means ± SE (standard error) and were subjected to a one-way ANOVA after data normality and homogeneity were verified using IBM 22.0 (IBM Corp., Michigan Avenue, Chicago, IL, USA).If the data did not exhibit homogeneity of variance, a nonparametric test (Kruskal-Wallis) was used for the comparison.The differences among treatments were identified, and Tukey's honest significant difference (HSD) test was used to compare the mean values; values of p < 0.05 were considered significant.

Serum Biochemical Indices
The serum biochemical indices are shown in Table 4.The different dietary n-3 HUFA levels did not affect serum AST, ALT, ALB, TP, GLU, and HDLC (p > 0.05).The serum activities of ALP reached their highest levels in fish fed with the 0.5% n-3 HUFA diet, and they were significantly higher than in fish fed with the 2.0% and 2.4% n-3 HUFA diets (p < 0.05).The contents of TCHO and LDLC in the serum increased, along with an increase in n-3 HUFA levels, and the contents of TCHO and LDLC in fish fed with the 1.5%, 2.0%, and 2.4% n-3 HUFA diets were significantly higher than in the fish fed with the 0.5% n-3 HUFA diet (p < 0.05).The TG contents reached their highest levels in the fish fed with the 1.5% n-3 HUFA diet, and they were significantly higher than in the other groups (p < 0.05).

Serum Antioxidant and Immunity Status
The activity of enzymes in the serum of Yangtze sturgeon is shown in Table 5.The T-AOC and CAT activities and MDA contents were not affected by the different contents of dietary n-3 HUFAs (p > 0.05).With an increase in the n-3 HUFA levels in the diet, the activity of SOD increased, reaching the maximum value in the fish fed the 2.4% n-3 HUFA diet; it was significantly higher than in the fish fed the 0.5% n-3 HUFA diet (p < 0.05).The GSH-Px activities in the fish fed the 2.4% n-3 HUFA diet were significantly higher than in the fish fed the 0.5% and 1.5% n-3 HUFA diets (p < 0.05).With an increase in the n-3 HUFA levels in the diet, the contents of IgM increased, reaching the maximum value in the fish fed the 1.0% n-3 HUFA diet; they were also significantly higher than in the fish fed the 0.5% n-3 HUFA diet (p < 0.05).The serum contents of C3 and C4 were not affected by dietary n-3 HUFAs (p > 0.05).

Muscle Antioxidant and Immunity Status
The activity of enzymes in the muscles of Yangtze sturgeon is shown in Table 6.With an increase in the n-3 HUFA levels in the diet, the activity of T-AOC increased, reaching a maximum value in fish fed the 2.0% n-3 HUFA diet; it was significantly higher than in the 0.5%, 1.0%, and 1.5% n-3 HUFA diet groups (p < 0.05).The activity of SOD also increased, reaching a maximum value in the 1.0% n-3 HUFA diet group; it was significantly higher than in the 0.50% and 2.4% n-3 HUFA diet groups (p < 0.05).The CAT activities in the 1.0% n-3 HUFA diet group were significantly higher than in the fish fed the 0.5% and 2.4% n-3 HUFA diets (p < 0.05).The content of MDA first increased and then decreased, reaching the minimum value in the 2.4% n-3 HUFA diet group; it was significantly lower than in the 0.5%, 1.0%, and 1.5% n-3 HUFA diet groups (p < 0.05).The muscle contents of IgM and C4 were not affected by the dietary n-3 HUFAs (p > 0.05).With an increase in the n-3 HUFA levels in the diet, the content of C3 decreased, reaching the minimum value in the fish fed the 2.4% n-3 HUFA diet; it was significantly lower than in the fish fed the 1.0% and 1.5% n-3 HUFA diets (p < 0.05).

Serum Oxylipin Profiles
The total ion current (TIC) overlap plot of the quality control samples (Supplementary Figure S3A), an integral correction diagram (Supplementary Figure S3B), and a canonical plot (Supplementary Figure S3C) are provided to ensure the accuracy of all oxylipins during the period of analysis.In the current study, a total of 80 oxylipins were detected in the serum of the Yangtze sturgeon (Supplementary Table S1).
After K-means cluster analyses, 62 oxylipins were clustered into 10 subclasses (Figure 1).Detailed classifications of these 62 oxylipins are presented in Supplementary Table S2.

Discussion
In this study, a feeding trial was conducted for up to 5 months to investigate the effects of n-3 HUFAs on Yangtze sturgeon.Interestingly, we found that this supplementation did not impact on important parameters such as WGR, FCR, CF, and SR.However, it is important to note that our conclusion remains unchanged-there is still a need to include EPA and DHA in the diets of sturgeon.This conclusion aligns with the findings of Luo et al. [12], who demonstrated that female Siberian sturgeon broodstocks with high levels of DHA experienced improved fecundity, egg hatchability, and, overall, enhanced larval quality.The authors also emphasized the significance of optimizing DHA/EPA ratios for optimal early development in Siberian sturgeon [13].Yoon et al. [14] reported that EPA and DHA play crucial roles in the growth and survival of lake sturgeon during their first year.Overall, we concluded that n-3 HUFAs have a significant role to play in broodstocks and in the early stages of sturgeon growth.Different developmental stages exhibit variations in nutrient utilization and selection.Asil et al. [30] pointed out that the rates of growth in the larval and juvenile stages are higher than in the maturation stage, wherein energy expenditure shifts away from weight gain and toward reproduction.In our study, the experimental fish were in ovarian stage II, which represents a significant developmental period relative to the lifespan of sturgeons.Although growth performance parameters were not affected by n-3 HUFAs, this does not mean that other physiological functions are not regulated by n-3 HUFAs.Certainly, the current feeding period of 5 months is relatively short for Yangtze sturgeons, which have an average initial body weight of 3.60 ± 0.83 kg.Additionally, it should be noted that the control group in this study contained 6.18% n-3 HUFAs, which may potentially mask the impact that a deficiency in n-3 HUFAs can have on female Yangtze sturgeons.Future studies should explore the effects of a diet lacking n-3 HUFAs and extended feeding periods on female Yangtze sturgeon to further elucidate their nutritional requirements.Similar observations have been made in juvenile Atlantic salmon (Salmo salar) [31], juvenile golden pompano (Trachinotus ovatus) [32], and turbot (Scophtalmus maximus L.) [33].
Serum biochemical indices serve as crucial indicators that reflect the health status of fish.A significant decrease in serum glucose (GLU) and total protein (TP) levels, along with elevated activities of alanine transaminase (ALT) and aspartate transaminase (AST), was reported by Lee [34] in rockfish (Sebastes schlegeli); these results were a consequence of dietary n-3 LC-PUFA deficiency.Furthermore, in starry flounder (Platichthys stellatus), an increase in AST activities was observed, whereas no impact was noted on serum ALT activities or GLU and TP concentrations, as reported by Lee [35].Mozanzadeh et al. [36] found relatively lower levels of serum GLU in fish fed diets containing 0.1% and 0.6% n-3 LC-PUFAs when comparing them with other groups; however, these diets did not affect serum TP and albumin (ALB) concentrations.However, ALP exhibited a contrasting pattern.Under the current experimental conditions, the serum levels of GLU, TP, and ALB concentrations and the activities of ALT and AST were consistent across all groups.However, a decrease in ALP activities accompanied the increase in dietary n-3 HUFA levels.These findings not only shed light on variations among different fish species but also indicate that the current diet for this species containing 6.18% n-3 HUFAs is sufficient and does not lead to any relevant deficiencies.Interestingly, the fish fed diets with 6.18% n-3 HUFAs had lower serum triglycerides (TGs) and LDL-C levels compared to the other groups; this observation contradicts the findings of previous studies conducted on turbot [33], silvery-black porgy (Sparidentex hasta) [36], juvenile Pacific white shrimp (Litopenaeus vannamei) [37], and Atlantic salmon [38].Simultaneously, lower serum TCHO contents were found in fish fed diets with 6.18% n-3 HUFAs.Similar phenomena were also observed in juvenile black seabream (Acanthopagrus schlegelii) and starry flounder [35,39].Lower levels of serum TCHO in fish fed diets with 6.18% n-3 HUFAs might be a consequence of the increased soybean oil content involved in the preparation of this diet.This is because, as Castro et al. [40] observed, cholesterol concentrations are lower in fish fed with vegetable oil (that has lower dietary n-3 HUFA levels) than fish fed with fish oil (that has higher dietary n-3 HUFA levels).One possible explanation for this difference is that the vegetable oil diets contain less cholesterol than fish oil; the levels observed in the control group can also be attributed to these oils themselves, as they are a major factor influencing cholesterol levels in fish fed such diets [39].
The antioxidant capacity of fish is primarily reflected through various antioxidant enzymes, which protect the fish body from damage by eliminating excessive reactive oxygen species (ROS) [41].Oxidative stress, a state of imbalance between oxidation and antioxidation, can have adverse effects on the fish's health [42].MDA serves as the primary product of lipid peroxidation [43], while the content of T-AOC represents the overall antioxidant level within the fish's body.Enzymes such as SOD, CAT, and GPx play a pivotal role in scavenging free radicals and alleviating oxidative stress [44].Under the experimental conditions of this study, it was observed that when the dietary content of n-3 HUFAs reached 2.4%, the activity of SOD in serum peaked.However, in muscle, this peak activity was achieved with a diet containing 1.0% n-3 HUFAs.This suggests that the optimal dietary requirements for n-3 HUFAs vary depending on the specific antioxidant enzyme and tissue type.Similarly, the significant increases in the CAT and TAC activities in serum required higher concentrations of n-3 HUFAs, whereas in muscle, a significant enhancement in these enzyme activities was observed in the fish fed diets containing 1% and 2% n-3 HUFAs compared to the control group.Notably, the muscle MDA content was significantly lower in fish fed diets with 2% to 2.4% n-3 HUFAs compared to the control group.In summary, the dietary requirements for n-3 HUFAs to exert different antioxidant enzyme activities in various tissues range from 1% to 2%.When the dietary n-3 HUFA content reaches 2%, the fish demonstrated a remarkable ability to eliminate lipid peroxides and maintain their overall health.Significantly increased T-AOC activities in the 1.24% group were observed in golden pompano fed diets with different n-3 HUFA levels [32].In the observation of largemouth bass (Micropterus salmoides) [3], slightly larger values of MDA were observed in fish fed diets with 1% compared to 2% EPA + DHA.Meanwhile, the serum contents of IgM and muscle C3 demonstrated an increase with 1%-1.5% n-3 HUFAs, followed by a decreasing trend at 2.4% n-3 HUFAs.Similar to the observations of juvenile golden pompano [32], they noted that the levels of IL-1β and IL-6 in serum and the gene mRNA expression of nf-κb, il-1β, il-6, and il-8 in the liver all decreased with an increase in the n-3 HUFA levels up to 2.1%.These results indicate that dietary supplementation with appropriate n-3 HUFA contents can effectively enhance the immune ability of fish.
Many studies have reported on oxylipin profiles in various species, including humans, rats, and even nonmammals.To the best of our knowledge, the effects of dietary long-chain PUFAs on fish oxylipin profiles have not been studied yet.In our study, we detected 80 oxylipins after feeding n-3 HUFA diets to Yangtze sturgeon for up to 5 months.Interestingly, 38.75% of these oxylipins were derived from ARA, indicating the majority.These findings are consistent with those observed in yellow croaker and rainbow trout [45].Additionally, a study conducted on rat hearts identified 75 oxylipins, two-thirds of which were derived from n-6 PUFA and two-thirds of these were formed ARA [46].The above message suggests that the main type of oxylipins in various animals may be quite similar (specifically, ARA oxylipins).These oxylipins typically exhibit a greater variety and quantity, indicating their potential vital roles [45].The oxylipins derived from N-6 polyunsaturated fatty acids (PUFAs) usually have proinflammatory, prothrombotic, and vasoconstrictive effects, but this is not absolute.On the contrary, the oxylipins derived from n-3 PUFAs often exhibit anti-inflammatory, vasodilatory, and antithrombotic effects [47,48].Notably, in the 1.0% n-3 HUFA diet group, a substantial proportion of DHA-derived oxylipins, including 4-HDHA, 7-HDHA, 8-HDHA, 10-HDHA, 14(S)-HDHA, and 16-HDHA, reached their peak, indicating that DHA can be efficiently converted into biologically active oxylipins under these dietary conditions.Conversely, in the 2.0% n-3 HUFA diet group, although some oxylipins still peaked, the proportion was relatively lower, potentially due to the regulation of fatty acid metabolic pathways.Similarly, in the 2.0% n-3 HUFA diet group, over half of the EPA-derived oxylipins reached a peak, particularly 5-HEPE and 14(15)-EpETE, whose levels increased significantly.These oxylipins likely possess specific biological functions that positively impact the physiological state of Yangtze sturgeon.Moreover, in the 1% to 2% n-3 HUFA diet groups, the ARA-derived oxylipins also exhibited a marked increase, further corroborating the intimate link between oxylipin production and the composition of fatty acids.Similar research also suggests that the concurrent elevation of plasma EPA levels along with plasma EPA-oxylipin levels is consistent with previous intervention studies, indicating that the oxylipin pattern reflects changes in the parent PUFA [49].These findings suggest that the peak responses of DHA-and EPA-derived oxylipins are primarily inconsistent, occurring within the range of 1-2% N-3 HUFAs.Furthermore, in diets containing 1-2% n-3 HUFAs, over 60% of ARA-derived oxylipin production also reached a peak.The peak response concentrations of these oxylipins mirror those observed in antioxidants and immune responses.The functions of these oxylipins significantly impact the antioxidant and immune status of the Yangtze sturgeon, with particular emphasis on the 14 oxylipins that exhibited significant increases, warranting further investigation.These oxylipins may maintain the Yangtze sturgeon's health by modulating antioxidant and immune responses.It is noteworthy that our study identified 14 oxylipins with significantly elevated levels, which may exert unique effects on the antioxidant and immune status of the Yangtze sturgeon.Therefore, future studies could delve deeper into the specific mechanisms of action of these oxylipins and their interactions with other biological molecules in jointly regulating the physiological processes of the Yangtze sturgeon.Generally, our study offers profound insights into the effects of n-3 HUFAs on the production of oxylipins in Yangtze sturgeon, serving as a valuable reference for the breeding and nutritional management of this species.By fine-tuning feed formulations and nutritional strategies, we can effectively modulate the levels of oxylipins in the Yangtze sturgeon, thereby enhancing its antioxidant and immune statuses, improving breeding efficiency and ensuring biological safety.

Conclusions
Adding n-3 HUFAs to the diet of Yangtze sturgeon can significantly boost their antioxidant capacity by strengthening the muscular activities of T-AOC, SOD, and CAT while simultaneously reducing muscle MDA content.Moreover, dietary n-3 HUFAs effectively mitigate physiological stress by elevating serum biochemical indicators, such as ALP, LDLC, TG, TCHO, GSH-Px, and SOD.Notably, the peak levels of most oxylipins occur with 1% to 2% N-3 HUFAs, which aligns with the requirements for optimal antioxidant and immune responses.Consequently, we suggest incorporating n-3 HUFAs into the diet at a concentration of 1.0 to 2.0% to enhance both the antioxidant capacity and overall physiological status of the sturgeon.These insights provide invaluable nutritional guidance for safeguarding this endangered species during its crucial stage II gonadal development.

Supplementary Materials:
The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/antiox13040421/s1.  S1: Liner equation and the profile of oxylipins in Yangtze sturgeon fed n-3 highly unsaturated fatty acids diets; Table S2: Classification of 62 oxylipins in Yangtze sturgeon fed n-3 highly unsaturated fatty acids diets.
Author Contributions: J.W. (Jinping Wu) and W.X. were responsible for experimental design and the writing of the original manuscript; W.L. and J.W. (Jinming Wu) were responsible for the feeding trial management and verifying the experimental data; R.R., P.F., Y.W., Y.L., X.L., P.L. and J.Z. were responsible for the feeding trial and sample analysis; C.Z. and H.D. for the funds support and review of the article.All authors have read and agreed to the published version of the manuscript.Informed Consent Statement: Not applicable.

Figure 1 .
Figure 1.K-means clustering of serum oxylipin in female F2-generation Yangtze sturgeon fed diets containing different levels of n-3 HUFAs.

Figure 1 .
Figure 1.K-means clustering of serum oxylipin in female F 2 -generation Yangtze sturgeon fed diets containing different levels of n-3 HUFAs.
Figure S1: The Yangtze sturgeon histological section of the stage II ovary; Figure S2: The amplified band of Yangtze sturgeon; Figure S3: The quality control of LC-MS/MS used in this study.(A) Total ions current (TIC) overlap plot of quality control samples; (B) Integral correction diagram; (C) Canonical plotting; Table

Funding:
The current experiment was funded by the Chong Qing Fishery Technology Innovation Union (CQFTIU2021-01), the Special Project for Performance Incentive Guidance of Research Institutions in Chongqing (cstc2022jxjl80011), the National Natural Science Foundation of China (32202932), and the Central Public-Interest Scientific Institution Basal Research Fund (2023TD08).Institutional Review Board Statement: All experimental fish and procedures in the current study complied with the Laboratory of Animal Experimental Ethics Inspection of the Laboratory Animal Centre, Yangtze River Fisheries Research Institute (2022YFI-WJP-01).

Table 1 .
Formulation and ingredients of the basal diet (%).

Table 2 .
Fatty acid composition of the experimental diets with different levels of n-3 HUFAs (% total fatty acids).

Table 3 .
Growth performance and feed utilization of female F 2 -generation Yangtze sturgeon fed diets containing different levels of n-3 HUFAs.
Data for IBW, FBW, WGR, FCR, and survival rate are presented as the means ± SE (n = 15), and the data for IBL, FBL, and CF are also presented as the means ± SE (n = 6).IBW, initial body weight; IBL, initial body length; FBW, final body weight; FBL, final body length; WGR, weight gain rate; FCR, feed conversion ratio; CF, condition factor.

Table 4 .
Serum biochemical indexes of female F2-generation Yangtze sturgeon fed diets containing different levels of n-3 HUFAs.

Table 5 .
Serum antioxidant and immunity statuses of female F2-generation Yangtze sturgeon fed diets containing different levels of n-3 HUFAs.

Table 6 .
Muscle antioxidant and immunity status of female F 2 -generation Yangtze sturgeon fed diets containing different levels of n-3 HUFAs.